Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0017149

Physical Location & Seq

Genomic Coordinates Reverse (-)

34039778 .. 34042221

Gene Structure 2444 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1205300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGGGGTGGGCAATAGCACTTCACGGCGGCGCAGGTGACATCCCGCTCTCTCTTCCACCTGAGCGGCGCCTGCCTCGCGAGGCCGGCCTCCGCCGCAGCCTCCACATCGGCGTCGAAGCTCTCAAAGCCAACACCCCACCTTTGGACGTCGTTGAGCTTGTGGTTAGGGAGCTAGAGAACATTCCACACTTCAATGCTGGAAAGGGGTCAGTGCTGACCACAGATGGCACTGTTGAAATGGAAGCTTGCATCATGGATGACAAAAAGAGATGTGGAGCTGTTTCTGGACTCACCACCGTCGTCAATCCCATCTCTTTAGCACGATTGGTCATGGAGAAAACTCCTCACATTTATCTTGCTTTTGATGGAGCAGAAGCCTTTGCTAGGGAACAGGGTGTTGAGACGCTAGATTCGAGTCATTTCATTACCCCGGAGAACGTAGAAAGGCTCAGTCAGGCAAAAGAAGCCAAGAGAGTTCAGATTGATTATACACAACCCCTTCACAAAGATGAGAAGAAAGAAACACCAGACGCTGATGGTGATAGTAAACTTGGGACTGTTGGATGCGTGGCTGTTGATAGTTTCGGAAATTTAGCTTCTGCAACTTCCACTGGCGGATTAGTCAACAAGATGGTTGGCCGGATTGGGGACACACCCATCATTGGTGCAGGGACATATGCCAACAATCTCTGCGCAGTTTCTGCTACTGGCAAAGGTGAAGCAATTATTCGTGGCACAGTTGCAAGGGATGTGGCAGCTCTTATGGAGTTCAAGGGTGTTTCTCTCAAAGAAGCAGCAGCTTATGTTGTGGAGGAGGGCACTCCAAAAGGCAATGTAGGGCTGGTTGCTGTGTCTGCCGCAGGGGAAGTCACGATGCCTTTCAATACCACCGGTATGTTTCGAGGTTGTGCCACTGAGGATGGGTATTCGGAGATTGCAATATGGCCTAATTCTGTGGAAAATGGAGTGGATAAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004067 GO:0004175 GO:0004190 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009627 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0010033 GO:0010035 GO:0012505 GO:0016787 GO:0016810 GO:0016811 GO:0019538 GO:0030163 GO:0033993 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0070001 GO:0070011 GO:0071704 GO:0097305 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asparaginase_2

Protein Analysis

327

Amino Acids

34.37

Weight (kDa)

5.2

Isoelectric Point (pI)

25.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asparaginase_2	PF01112	1 - 314	4e-112	Asparaginase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017149)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08100
fragaria_vesca	FvH4_5g13420
malus_domestica	MD06G1205300.v1.1 MD06G1205500.v1.1
prunus_persica	Prupe.5G209700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0183691
rosa_laevigata	RLG00000005032
rosa_multiflora	Rmu_sc0003610.1_g000026
rosa_roxburghii	Rroxscaffold_3G00270320
rosa_rugosa	Rorug06G0459400
rosa_samantha	Rh7BG060300 Rh7DG060200
rosa_wichuraiana	Rw7G004990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	23
AatII	GACGTC	1 cut(s)	150
Acc16I	TGCGCA	1 cut(s)	694
Acc36I	ACCTGC	1 cut(s)	23
AccB1I	GGYRCC	1 cut(s)	66
AccBSI	CCGCTC	2 cut(s)	46, 64
AccII	CGCG	1 cut(s)	78
AciI	CCGC	7 cut(s)	27, 44, 64, 91, 94, 615, 858
AcoI	YGGCCR	1 cut(s)	637
AcsI	RAATTY	1 cut(s)	589
AcyI	GRCGYC	3 cut(s)	67, 111, 147
AfiI	CCNNNNNNNGG	4 cut(s)	142, 384, 646, 662
AgeI	ACCGGT	1 cut(s)	890
AgsI	TTSAA	4 cut(s)	193, 236, 772, 883
AluBI	AGCT	8 cut(s)	119, 157, 172, 245, 278, 596, 758, 800
AluI	AGCT	8 cut(s)	119, 157, 172, 245, 278, 596, 758, 800
Alw26I	GTCTC	1 cut(s)	395
AlwNI	CAGNNNCTG	2 cut(s)	533, 701
AoxI	GGCC	4 cut(s)	81, 85, 637, 944
ApeKI	GCWGC	4 cut(s)	96, 755, 794, 797
ApoI	RAATTY	1 cut(s)	589
ArsI	GACNNNNNNTTYG	2 cut(s)	394, 426
AsiGI	ACCGGT	1 cut(s)	890
AspLEI	GCGC	3 cut(s)	32, 69, 695
AsuC2I	CCSGG	1 cut(s)	431
AsuHPI	GGTGA	4 cut(s)	47, 283, 551, 728
BaeGI	GKGCMC	1 cut(s)	821
BanI	GGYRCC	1 cut(s)	66
BbvI	GCAGC	4 cut(s)	108, 767, 806, 809
BccI	CCATC	7 cut(s)	218, 317, 359, 530, 625, 665, 914
BceAI	ACGGC	1 cut(s)	40
BcnI	CCSGG	1 cut(s)	431
BcoDI	GTCTC	1 cut(s)	395
BfaI	CTAG	3 cut(s)	173, 384, 407
BfoI	RGCGCY	1 cut(s)	70
BfuAI	ACCTGC	1 cut(s)	23
BisI	GCNGC	8 cut(s)	28, 65, 94, 97, 756, 795, 798, 858
BlsI	GCNGC	8 cut(s)	29, 66, 95, 98, 757, 796, 799, 859
Bme1390I	CCNGG	1 cut(s)	431
BmiI	GGNNCC	1 cut(s)	68
BmrFI	CCNGG	1 cut(s)	431
BmsI	GCATC	3 cut(s)	258, 554, 864
Bpu10I	CCTNAGC	1 cut(s)	60
BpuMI	CCSGG	1 cut(s)	431
BsaHI	GRCGYC	3 cut(s)	67, 111, 147
BsaJI	CCNNGG	1 cut(s)	429
BsaWI	WCCGGW	1 cut(s)	890
Bsc4I	CCNNNNNNNGG	4 cut(s)	142, 384, 646, 662
Bse118I	RCCGGY	2 cut(s)	83, 890
Bse1I	ACTGG	2 cut(s)	616, 712
Bse3DI	GCAATG	1 cut(s)	838
BseDI	CCNNGG	1 cut(s)	429
BseGI	GGATG	5 cut(s)	39, 262, 569, 754, 925
BseLI	CCNNNNNNNGG	4 cut(s)	142, 384, 646, 662
BseMI	GCAATG	1 cut(s)	838
BseMII	CTCAG	3 cut(s)	51, 463, 906
BseNI	ACTGG	2 cut(s)	616, 712
BseRI	GAGGAG	2 cut(s)	333, 827
BseSI	GKGCMC	1 cut(s)	821
BseXI	GCAGC	4 cut(s)	108, 767, 806, 809
BsgI	GTGCAG	1 cut(s)	687
Bsh1236I	CGCG	1 cut(s)	78
BshFI	GGCC	4 cut(s)	83, 87, 639, 946
BshNI	GGYRCC	1 cut(s)	66
BshTI	ACCGGT	1 cut(s)	890
BsiSI	CCGG	4 cut(s)	84, 431, 640, 891
BslFI	GGGAC	3 cut(s)	568, 662, 685
BslI	CCNNNNNNNGG	4 cut(s)	142, 384, 646, 662
BsmAI	GTCTC	1 cut(s)	395
BsmBI	CGTCTC	1 cut(s)	395
BsmFI	GGGAC	3 cut(s)	568, 662, 685
BsnI	GGCC	4 cut(s)	83, 87, 639, 946
Bsp1286I	GDGCHC	1 cut(s)	821
Bsp68I	TCGCGA	1 cut(s)	78
BspACI	CCGC	7 cut(s)	27, 44, 64, 91, 94, 615, 858
BspANI	GGCC	4 cut(s)	83, 87, 639, 946
BspCNI	CTCAG	3 cut(s)	52, 462, 907
BspFNI	CGCG	1 cut(s)	78
BspLI	GGNNCC	1 cut(s)	68
BspMI	ACCTGC	1 cut(s)	23
BspT107I	GGYRCC	1 cut(s)	66
BsrBI	CCGCTC	2 cut(s)	46, 64
BsrDI	GCAATG	1 cut(s)	838
BsrFI	RCCGGY	2 cut(s)	83, 890
BsrI	ACTGG	2 cut(s)	616, 712
BssAI	RCCGGY	2 cut(s)	83, 890
BssECI	CCNNGG	1 cut(s)	429
BssNI	GRCGYC	3 cut(s)	67, 111, 147
Bst4CI	ACNGT	4 cut(s)	232, 298, 559, 739
Bst6I	CTCTTC	1 cut(s)	57
BstACI	GRCGYC	3 cut(s)	67, 111, 147
BstAPI	GCANNNNNTGC	1 cut(s)	701
BstC8I	GCNNGC	3 cut(s)	71, 85, 247
BstDEI	CTNAG	3 cut(s)	60, 449, 915
BstENI	CCTNNNNNAGG	1 cut(s)	382
BstF5I	GGATG	5 cut(s)	39, 262, 569, 754, 925
BstFNI	CGCG	1 cut(s)	78
BstH2I	RGCGCY	1 cut(s)	70
BstHHI	GCGC	3 cut(s)	32, 69, 695
BstMAI	GTCTC	1 cut(s)	395
BstMWI	GCNNNNNNNGC	8 cut(s)	70, 75, 84, 93, 125, 464, 701, 854
BstSCI	CCNGG	1 cut(s)	429
BstSLI	GKGCMC	1 cut(s)	821
BstUI	CGCG	1 cut(s)	78
BstV1I	GCAGC	4 cut(s)	108, 767, 806, 809
BsuRI	GGCC	4 cut(s)	83, 87, 639, 946
BtsCI	GGATG	5 cut(s)	39, 262, 569, 754, 925
BtsIMutI	CAGTG	4 cut(s)	216, 228, 609, 912
BtuMI	TCGCGA	1 cut(s)	78
BveI	ACCTGC	1 cut(s)	23
Cac8I	GCNNGC	3 cut(s)	71, 85, 247
CaiI	CAGNNNCTG	2 cut(s)	533, 701
CfoI	GCGC	3 cut(s)	32, 69, 695
Cfr10I	RCCGGY	2 cut(s)	83, 890
CseI	GACGC	3 cut(s)	100, 412, 539
CspAI	ACCGGT	1 cut(s)	890
CspCI	CAANNNNNGTGG	2 cut(s)	732, 767
CviAII	CATG	2 cut(s)	253, 331
DdeI	CTNAG	3 cut(s)	60, 449, 915
DinI	GGCGCC	1 cut(s)	68
EaeI	YGGCCR	1 cut(s)	637
Eam1104I	CTCTTC	1 cut(s)	57
EarI	CTCTTC	1 cut(s)	57
EciI	GGCGGA	2 cut(s)	80, 630
EcoNI	CCTNNNNNAGG	1 cut(s)	382
EgeI	GGCGCC	1 cut(s)	68
EheI	GGCGCC	1 cut(s)	68
Esp3I	CGTCTC	1 cut(s)	395
FaeI	CATG	2 cut(s)	256, 334
FaiI	YATR	9 cut(s)	254, 332, 489, 676, 678, 764, 804, 896, 943
FaqI	GGGAC	3 cut(s)	568, 662, 685
FatI	CATG	2 cut(s)	252, 330
FauI	CCCGC	1 cut(s)	51
FauNDI	CATATG	1 cut(s)	676
Fnu4HI	GCNGC	8 cut(s)	28, 65, 94, 97, 756, 795, 798, 858
FokI	GGATG	5 cut(s)	26, 269, 576, 761, 932
FseI	GGCCGGCC	1 cut(s)	87
Fsp4HI	GCNGC	8 cut(s)	28, 65, 94, 97, 756, 795, 798, 858
FspBI	CTAG	3 cut(s)	173, 384, 407
FspI	TGCGCA	1 cut(s)	694
GlaI	GCGC	3 cut(s)	31, 68, 694
GluI	GCNGC	8 cut(s)	28, 65, 94, 97, 756, 795, 798, 858
HaeII	RGCGCY	1 cut(s)	70
HaeIII	GGCC	4 cut(s)	83, 87, 639, 946
HapII	CCGG	4 cut(s)	84, 431, 640, 891
HgaI	GACGC	3 cut(s)	100, 412, 539
HhaI	GCGC	3 cut(s)	32, 69, 695
Hin1I	GRCGYC	3 cut(s)	67, 111, 147
Hin1II	CATG	2 cut(s)	256, 334
Hin6I	GCGC	3 cut(s)	30, 67, 693
HinP1I	GCGC	3 cut(s)	30, 67, 693
HincII	GTYRAC	1 cut(s)	625
HindII	GTYRAC	1 cut(s)	625
HindIII	AAGCTT	1 cut(s)	243
HinfI	GANTC	3 cut(s)	288, 410, 415
HpaII	CCGG	4 cut(s)	84, 431, 640, 891
HphI	GGTGA	4 cut(s)	47, 283, 551, 728
Hpy166II	GTNNAC	2 cut(s)	548, 625
Hpy188I	TCNGA	3 cut(s)	480, 587, 931
Hpy188III	TCNNGA	3 cut(s)	77, 285, 871
Hpy8I	GTNNAC	2 cut(s)	548, 625
Hpy99I	CGWCG	3 cut(s)	116, 152, 302
HpyAV	CCTTC	1 cut(s)	509
HpyCH4III	ACNGT	4 cut(s)	232, 298, 559, 739
HpyCH4IV	ACGT	2 cut(s)	147, 438
HpyCH4V	TGCA	5 cut(s)	249, 602, 668, 743, 938
HpyF10VI	GCNNNNNNNGC	8 cut(s)	70, 75, 84, 93, 125, 464, 701, 854
HpyF3I	CTNAG	3 cut(s)	60, 449, 915
HpySE526I	ACGT	2 cut(s)	147, 438
Hsp92I	GRCGYC	3 cut(s)	67, 111, 147
Hsp92II	CATG	2 cut(s)	256, 334
HspAI	GCGC	3 cut(s)	30, 67, 693
KasI	GGCGCC	1 cut(s)	66
KroI	GCCGGC	1 cut(s)	83
KroNI	GCCGGC	1 cut(s)	85
LmnI	GCTCC	3 cut(s)	169, 275, 368
Lsp1109I	GCAGC	4 cut(s)	108, 767, 806, 809
LweI	GCATC	3 cut(s)	258, 554, 864
MaeI	CTAG	3 cut(s)	173, 384, 407
MaeII	ACGT	2 cut(s)	147, 438
MaeIII	GTNAC	2 cut(s)	35, 868
MbiI	CCGCTC	2 cut(s)	46, 64
MboII	GAAGA	2 cut(s)	44, 526
MhlI	GDGCHC	1 cut(s)	821
MluCI	AATT	3 cut(s)	589, 723, 949
Mly113I	GGCGCC	1 cut(s)	67
MlyI	GAGTC	2 cut(s)	282, 424
MmeI	TCCRAC	1 cut(s)	541
MnlI	CCTC	9 cut(s)	73, 84, 98, 110, 354, 805, 808, 896, 910
MroNI	GCCGGC	1 cut(s)	83
MslI	CAYNNNNRTG	3 cut(s)	192, 507, 893
MspI	CCGG	4 cut(s)	84, 431, 640, 891
MspR9I	CCNGG	1 cut(s)	431
MvnI	CGCG	1 cut(s)	78
MwoI	GCNNNNNNNGC	8 cut(s)	70, 75, 84, 93, 125, 464, 701, 854
NaeI	GCCGGC	1 cut(s)	85
NarI	GGCGCC	1 cut(s)	67
NciI	CCSGG	1 cut(s)	431
NdeI	CATATG	1 cut(s)	676
NgoMIV	GCCGGC	1 cut(s)	83
NlaIII	CATG	2 cut(s)	256, 334
NlaIV	GGNNCC	1 cut(s)	68
NmuCI	GTSAC	2 cut(s)	35, 868
NruI	TCGCGA	1 cut(s)	78
NsbI	TGCGCA	1 cut(s)	694
PaqCI	CACCTGC	1 cut(s)	23
PcsI	WCGNNNNNNNCGW	1 cut(s)	410
PdiI	GCCGGC	1 cut(s)	85
PfeI	GAWTC	1 cut(s)	410
PinAI	ACCGGT	1 cut(s)	890
PkrI	GCNGC	8 cut(s)	29, 66, 95, 98, 757, 796, 799, 859
PleI	GAGTC	2 cut(s)	282, 423
PluTI	GGCGCC	1 cut(s)	70
PpsI	GAGTC	2 cut(s)	282, 423
PspN4I	GGNNCC	1 cut(s)	68
PstNI	CAGNNNCTG	2 cut(s)	533, 701
RigI	GGCCGGCC	1 cut(s)	87
RruI	TCGCGA	1 cut(s)	78
RseI	CAYNNNNRTG	3 cut(s)	192, 507, 893
SatI	GCNGC	8 cut(s)	28, 65, 94, 97, 756, 795, 798, 858
SchI	GAGTC	2 cut(s)	282, 424
ScrFI	CCNGG	1 cut(s)	431
SduI	GDGCHC	1 cut(s)	821
SfaNI	GCATC	3 cut(s)	258, 554, 864
SfoI	GGCGCC	1 cut(s)	68
SmiMI	CAYNNNNRTG	3 cut(s)	192, 507, 893
Sse9I	AATT	3 cut(s)	589, 723, 949
SsiI	CCGC	7 cut(s)	27, 44, 64, 91, 94, 615, 858
SspDI	GGCGCC	1 cut(s)	66
SspMI	CTAG	3 cut(s)	173, 384, 407
StyD4I	CCNGG	1 cut(s)	429
TaaI	ACNGT	4 cut(s)	232, 298, 559, 739
TaiI	ACGT	2 cut(s)	150, 441
TaqI	TCGA	3 cut(s)	114, 413, 901
TasI	AATT	3 cut(s)	589, 723, 949
TauI	GCSGC	4 cut(s)	30, 67, 96, 860
TfiI	GAWTC	1 cut(s)	410
TscAI	CASTG	4 cut(s)	216, 235, 616, 919
TseFI	GTSAC	2 cut(s)	35, 868
TseI	GCWGC	4 cut(s)	96, 755, 794, 797
Tsp45I	GTSAC	2 cut(s)	35, 868
TspDTI	ATGAA	1 cut(s)	412
TspRI	CASTG	4 cut(s)	216, 235, 616, 919
XagI	CCTNNNNNAGG	1 cut(s)	382
XapI	RAATTY	1 cut(s)	589
XspI	CTAG	3 cut(s)	173, 384, 407
ZraI	GACGTC	1 cut(s)	148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.