RLG00000005032

Isoaspartyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017149
Physical Location & Seq
Forward (+)
64456621 .. 64458091
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005032

Sequence Viewer

Length: 966 bp
ATGGGGTGGGCCATCGCACTCCACGGCGGCGCCGGCGACATCCCGCTCTCTCTTCAGCCGGAGCGCCGCCAGCCTCGCGAGGCCGCCCTCCGCCACTGCCTCCAGATCGGCGTCGAAGCTCTCAAGGCCAACAAGCCTCCTCTCGACGTCGTCGAGCTTGTGGTTCGTGAGCTGGAGAATATTCCGGTGTTCAACGCCGGAAAGGGGTCCGTGTTGACCAGCAGCGGCACGGTGGAGATGGAAGCTTGCATTATGGACGGCACAAAGAGATGTGGAGCTGTTTCTGGCCTCACTACTGTAGTCAATCCCATATCTTTGGCCAGATTGGTCATGGAGAACACTCCTCATATCTATCTTGCTTTTGATGGAGCTGAGGCCTTTGCTAGGGAACAAGGCGTTGAGTGTGTAGATGCGAATAGCTTCATTACCGCGGAGAATATTGAGAGGCTAAAGCAGGCAAAAAAGGCCAACAGAGTGCAGATTGATTATACACAACCGATTAAGAAAGATGTGAAGGAAGAAACACCAGAAGCTGATGGTGATAGCCAGCTTGGAACTGTTGGATGTGTGGCTGTTGATGATCTTGGGAATTTAGCTTCTGCAACTTCAACAGGCGGATTAGTCAACAAGATGGTTGGCCGGATTGGGGACACACCTATTATCGGTGCAGGGACGTATGCCAACAATCTCTGTGCAGTTTCTGCCACTGGCAAAGGTGAAGCAATAATCCGGAGTACAGCTGCGAGGGATGTAGCAGCTTTTATGGAGTACAAAGGTGTTTCTCTCAAAGAGGCTGCAGCTTATGTTGTGGAGGAGAGCACTCCAAAAGGCAATGTAGGCTTGGTTGCTGTATCTGCCAAAGGAGAAGTCACAATGGCTTTCAATACAACTGGGATGTTTCGAGCTTGCGCTACTGAGGATGGGTATTGGGAGATTGGAATATGGCCTTCAGATGTGCAGAAATGA

Protein Analysis

322

Amino Acids

33.89

Weight (kDa)

5.06

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 1 - 313 1.1e-113 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08100
fragaria_vesca FvH4_5g13420
malus_domestica MD06G1205300.v1.1 MD06G1205500.v1.1
prunus_persica Prupe.5G209700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0183691
rosa_laevigata RLG00000005032
rosa_multiflora Rmu_sc0003610.1_g000026
rosa_roxburghii Rroxscaffold_3G00270320
rosa_rugosa Rorug06G0459400
rosa_samantha Rh7BG060300 Rh7DG060200
rosa_wichuraiana Rw7G004990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 29
AccBSI CCGCTC 1 cut(s) 46
AccII CGCG 2 cut(s) 78, 431
AccIII TCCGGA 1 cut(s) 729
AciI CCGC 9 cut(s) 27, 44, 67, 84, 91, 225, 429, 431, 615
AcoI YGGCCR 2 cut(s) 318, 637
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 2 cut(s) 38, 933
AcyI GRCGYC 3 cut(s) 30, 111, 147
AfaI GTAC 2 cut(s) 736, 770
AfiI CCNNNNNNNGG 4 cut(s) 204, 384, 646, 662
AgsI TTSAA 3 cut(s) 193, 609, 883
Alw21I GWGCWC 1 cut(s) 821
AlwNI CAGNNNCTG 2 cut(s) 533, 701
Aor13HI TCCGGA 1 cut(s) 729
AoxI GGCC 9 cut(s) 9, 81, 126, 286, 318, 375, 465, 637, 944
ApeKI GCWGC 5 cut(s) 222, 740, 755, 794, 797
ApoI RAATTY 1 cut(s) 589
ArsI GACNNNNNNTTYG 2 cut(s) 852, 884
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 3 cut(s) 32, 66, 911
AspS9I GGNCC 2 cut(s) 9, 207
AsuHPI GGTGA 2 cut(s) 551, 728
AvaII GGWCC 1 cut(s) 207
BalI TGGCCA 1 cut(s) 320
BanI GGYRCC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 821
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 5 cut(s) 234, 727, 767, 781, 809
BccI CCATC 6 cut(s) 20, 232, 359, 530, 625, 914
BceAI ACGGC 2 cut(s) 40, 274
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 297, 795
BfoI RGCGCY 2 cut(s) 33, 67
BisI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
BlsI GCNGC 9 cut(s) 29, 68, 85, 224, 227, 742, 757, 796, 799
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 9, 207
BmiI GGNNCC 2 cut(s) 31, 208
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 1 cut(s) 400
BmuI ACTGGG 1 cut(s) 900
BpmI CTGGAG 2 cut(s) 86, 194
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 107
BsaHI GRCGYC 3 cut(s) 30, 111, 147
BsaJI CCNNGG 2 cut(s) 22, 429
BsaWI WCCGGW 2 cut(s) 184, 729
Bsc4I CCNNNNNNNGG 4 cut(s) 204, 384, 646, 662
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 2 cut(s) 712, 895
Bse3DI GCAATG 1 cut(s) 838
BseAI TCCGGA 1 cut(s) 729
BseDI CCNNGG 2 cut(s) 22, 429
BseGI GGATG 5 cut(s) 39, 569, 754, 900, 925
BseLI CCNNNNNNNGG 4 cut(s) 204, 384, 646, 662
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 2 cut(s) 363, 906
BseNI ACTGG 2 cut(s) 712, 895
BseRI GAGGAG 3 cut(s) 129, 333, 827
BseXI GCAGC 5 cut(s) 234, 727, 767, 781, 809
BsgI GTGCAG 3 cut(s) 497, 687, 714
Bsh1236I CGCG 2 cut(s) 78, 431
BshFI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
BshNI GGYRCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 821
BsiSI CCGG 6 cut(s) 33, 59, 185, 198, 640, 730
BslFI GGGAC 2 cut(s) 662, 685
BslI CCNNNNNNNGG 4 cut(s) 204, 384, 646, 662
BsmFI GGGAC 2 cut(s) 662, 685
BsnI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
Bsp1286I GDGCHC 1 cut(s) 821
Bsp13I TCCGGA 1 cut(s) 729
Bsp143I GATC 2 cut(s) 105, 580
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 9 cut(s) 27, 44, 67, 84, 91, 225, 429, 431, 615
BspANI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
BspCNI CTCAG 2 cut(s) 364, 907
BspEI TCCGGA 1 cut(s) 729
BspFNI CGCG 2 cut(s) 78, 431
BspLI GGNNCC 2 cut(s) 31, 208
BspMAI CTGCAG 1 cut(s) 799
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 2 cut(s) 712, 895
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 22, 429
BssMI GATC 2 cut(s) 105, 580
BssNI GRCGYC 3 cut(s) 30, 111, 147
Bst4CI ACNGT 3 cut(s) 232, 298, 559
Bst6I CTCTTC 1 cut(s) 57
BstACI GRCGYC 3 cut(s) 30, 111, 147
BstAPI GCANNNNNTGC 1 cut(s) 701
BstC8I GCNNGC 6 cut(s) 34, 71, 247, 456, 548, 907
BstDEI CTNAG 2 cut(s) 372, 915
BstDSI CCRYGG 2 cut(s) 22, 429
BstENI CCTNNNNNAGG 1 cut(s) 382
BstF5I GGATG 5 cut(s) 39, 569, 754, 900, 925
BstFNI CGCG 2 cut(s) 78, 431
BstH2I RGCGCY 2 cut(s) 33, 67
BstHHI GCGC 3 cut(s) 32, 66, 911
BstKTI GATC 2 cut(s) 108, 583
BstMBI GATC 2 cut(s) 105, 580
BstMWI GCNNNNNNNGC 8 cut(s) 33, 70, 75, 125, 464, 701, 837, 854
BstSFI CTRYAG 2 cut(s) 297, 795
BstUI CGCG 2 cut(s) 78, 431
BstV1I GCAGC 5 cut(s) 234, 727, 767, 781, 809
BstXI CCANNNNNNTGG 1 cut(s) 316
BsuRI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
BtgI CCRYGG 2 cut(s) 22, 429
BtsCI GGATG 5 cut(s) 39, 569, 754, 900, 925
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 94, 705
BtuMI TCGCGA 1 cut(s) 78
Cac8I GCNNGC 6 cut(s) 34, 71, 247, 456, 548, 907
CaiI CAGNNNCTG 2 cut(s) 533, 701
CfoI GCGC 3 cut(s) 32, 66, 911
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 9, 207
Cfr42I CCGCGG 1 cut(s) 432
CseI GACGC 1 cut(s) 100
Csp6I GTAC 2 cut(s) 735, 769
CviAII CATG 1 cut(s) 331
CviQI GTAC 2 cut(s) 735, 769
DdeI CTNAG 2 cut(s) 372, 915
DinI GGCGCC 1 cut(s) 31
DpnI GATC 2 cut(s) 107, 582
DpnII GATC 2 cut(s) 105, 580
EaeI YGGCCR 2 cut(s) 318, 637
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 2 cut(s) 80, 630
Eco147I AGGCCT 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 207
Eco57I CTGAAG 2 cut(s) 38, 933
EcoNI CCTNNNNNAGG 1 cut(s) 382
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
FaeI CATG 1 cut(s) 334
FaiI YATR 9 cut(s) 254, 311, 332, 348, 489, 678, 764, 804, 943
FaqI GGGAC 2 cut(s) 662, 685
FatI CATG 1 cut(s) 330
FauI CCCGC 1 cut(s) 51
Fnu4HI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
FokI GGATG 5 cut(s) 26, 576, 761, 907, 932
Fsp4HI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
FspBI CTAG 1 cut(s) 384
GlaI GCGC 3 cut(s) 31, 65, 910
GluI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
GsuI CTGGAG 2 cut(s) 86, 194
HaeII RGCGCY 2 cut(s) 33, 67
HaeIII GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
HapII CCGG 6 cut(s) 33, 59, 185, 198, 640, 730
HgaI GACGC 1 cut(s) 100
HhaI GCGC 3 cut(s) 32, 66, 911
Hin1I GRCGYC 3 cut(s) 30, 111, 147
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 3 cut(s) 30, 64, 909
HinP1I GCGC 3 cut(s) 30, 64, 909
HincII GTYRAC 2 cut(s) 216, 625
HindII GTYRAC 2 cut(s) 216, 625
HindIII AAGCTT 1 cut(s) 243
HpaII CCGG 6 cut(s) 33, 59, 185, 198, 640, 730
HphI GGTGA 2 cut(s) 551, 728
Hpy166II GTNNAC 2 cut(s) 216, 625
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 5 cut(s) 77, 103, 143, 167, 730
Hpy8I GTNNAC 2 cut(s) 216, 625
Hpy99I CGWCG 4 cut(s) 116, 149, 152, 155
HpyAV CCTTC 2 cut(s) 508, 957
HpyCH4III ACNGT 3 cut(s) 232, 298, 559
HpyCH4IV ACGT 2 cut(s) 147, 674
HpyCH4V TGCA 7 cut(s) 249, 478, 602, 668, 695, 797, 958
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 70, 75, 125, 464, 701, 837, 854
HpyF3I CTNAG 2 cut(s) 372, 915
HpySE526I ACGT 2 cut(s) 147, 674
Hsp92I GRCGYC 3 cut(s) 30, 111, 147
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 3 cut(s) 30, 64, 909
KasI GGCGCC 1 cut(s) 29
Kpn2I TCCGGA 1 cut(s) 729
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
KspI CCGCGG 1 cut(s) 432
Kzo9I GATC 2 cut(s) 105, 580
LmnI GCTCC 3 cut(s) 61, 275, 368
Lsp1109I GCAGC 5 cut(s) 234, 727, 767, 781, 809
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 147, 674
MaeIII GTNAC 1 cut(s) 868
MalI GATC 2 cut(s) 107, 582
MbiI CCGCTC 1 cut(s) 46
MboI GATC 2 cut(s) 105, 580
MboII GAAGA 2 cut(s) 44, 530
MhlI GDGCHC 1 cut(s) 821
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 1 cut(s) 589
MluNI TGGCCA 1 cut(s) 320
Mly113I GGCGCC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 541
Mox20I TGGCCA 1 cut(s) 320
MreI CGCCGGCG 1 cut(s) 32
MroI TCCGGA 1 cut(s) 729
MroNI GCCGGC 1 cut(s) 32
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 3 cut(s) 225, 431, 740
MspI CCGG 6 cut(s) 33, 59, 185, 198, 640, 730
MvnI CGCG 2 cut(s) 78, 431
MwoI GCNNNNNNNGC 8 cut(s) 33, 70, 75, 125, 464, 701, 837, 854
NaeI GCCGGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 30
NdeII GATC 2 cut(s) 105, 580
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 1 cut(s) 334
NlaIV GGNNCC 2 cut(s) 31, 208
NmuCI GTSAC 1 cut(s) 868
NruI TCGCGA 1 cut(s) 78
PceI AGGCCT 1 cut(s) 377
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 419
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 9 cut(s) 29, 68, 85, 224, 227, 742, 757, 796, 799
PluTI GGCGCC 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 31, 208
PspPI GGNCC 2 cut(s) 9, 207
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 2 cut(s) 533, 701
PsyI GACNNNGTC 1 cut(s) 149
PvuII CAGCTG 1 cut(s) 740
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 2 cut(s) 736, 770
RsaNI GTAC 2 cut(s) 735, 769
SacII CCGCGG 1 cut(s) 432
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
Sau3AI GATC 2 cut(s) 105, 580
Sau96I GGNCC 2 cut(s) 9, 207
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 2 cut(s) 297, 795
SfoI GGCGCC 1 cut(s) 31
Sfr303I CCGCGG 1 cut(s) 432
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 432
SinI GGWCC 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 589
SseBI AGGCCT 1 cut(s) 377
SsiI CCGC 9 cut(s) 27, 44, 67, 84, 91, 225, 429, 431, 615
SspDI GGCGCC 1 cut(s) 29
SspI AATATT 2 cut(s) 181, 439
SspMI CTAG 1 cut(s) 384
StuI AGGCCT 1 cut(s) 377
TaaI ACNGT 3 cut(s) 232, 298, 559
TaiI ACGT 2 cut(s) 150, 677
TaqI TCGA 4 cut(s) 114, 144, 153, 901
TasI AATT 1 cut(s) 589
TatI WGTACW 2 cut(s) 734, 768
TauI GCSGC 4 cut(s) 30, 69, 86, 228
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 101, 712
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 5 cut(s) 222, 740, 755, 794, 797
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 1 cut(s) 412
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 2 cut(s) 101, 712
Tth111I GACNNNGTC 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 207
XagI CCTNNNNNAGG 1 cut(s) 382
XapI RAATTY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 384
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.