Rroxscaffold_3G00270320

Isoaspartyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017149
Physical Location & Seq
Forward (+)
62910159 .. 62911834
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270320.1

Sequence Viewer

Length: 966 bp
ATGGGATGGGCCATCGCACTCCACGGCGGCGCCGGCGACATCCCGCTCTCTCTTCCGCCTGAGCGCCGCCAGCCTCGCGAGGCCGCCCTCCGCCACTGCCTCCAGATGGGCGTCGAAGCTCTCAAGGCCAACAAGCCTCCTCTCGACGTCGTCGAGCTTGTGGTTCGTGAGCTGGAGAATATTCCGGTGTTCAACGCCGGAAAGGGGTCTGTGTTGACCAGCAGCGGCACGGTGGAGATGGAAGCTTGTATTATGGACGGCACTAAGAGATGTGGAGCTGTTTCTGGCCTCACTACTGTAGTCAATCCCATATCTTTGGCCAGATTGGTGATGGAGAACACTCCTCATATCTATCTTGCTTTTGATGGAGCTGAGGCCTTTGCTAGGGAACAAGGCGTTGAGTGTGTAGATGCGAATAGCTTCATTACCGCGGAGAATGTTGAGAGGCTAAAGCAGGCAAAAGAGGCCAACAGAGTGCAGATTGATTATACACAACCGATTAAGAAAGATGTGAAGGAAGAAACACCAGAAGCTGATGGTGATAGCCAGCTTGGAACTGTTGGATGTGTGGCTGTTGATGATCTTGGGAATTTAGCTTCTGCAACTTCAACAGGCGGATTAGTCAACAAGATGGTTGGCCGGATTGGGGACACACCTATTATCGGTGCAGGGACGTATGCCAACAATCTCTGTGCAGTTTCTGCCACTGGCAAAGGTGAAGCAATAATCCGGAGTACAGCTGCGAGGGATGTGGCAGCTTTTATGGAGTACAAAGGTGTTTCTCTCAAAGAGGCTGCAGCTTATGTTGTGGAGGAGAGCACTCCAAAAGGCAATGTAGGCTTGGTTGCTGTATCTGCCAAAGGAGAAGTCACAATGCCTTTCAATACAACAGGGATGTTTCGAGCTTGTGCTACTGAGGATGGGTATTGGGAGATTGGAATATGGCCTTCTGATGTGCAAAAATGA

Protein Analysis

321

Amino Acids

33.89

Weight (kDa)

4.93

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 1 - 313 2e-114 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08100
fragaria_vesca FvH4_5g13420
malus_domestica MD06G1205300.v1.1 MD06G1205500.v1.1
prunus_persica Prupe.5G209700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0183691
rosa_laevigata RLG00000005032
rosa_multiflora Rmu_sc0003610.1_g000026
rosa_roxburghii Rroxscaffold_3G00270320
rosa_rugosa Rorug06G0459400
rosa_samantha Rh7BG060300 Rh7DG060200
rosa_wichuraiana Rw7G004990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 29
AccBSI CCGCTC 1 cut(s) 46
AccII CGCG 2 cut(s) 78, 431
AccIII TCCGGA 1 cut(s) 729
AcoI YGGCCR 2 cut(s) 318, 637
AcsI RAATTY 1 cut(s) 589
AcyI GRCGYC 3 cut(s) 30, 111, 147
AfaI GTAC 2 cut(s) 736, 770
AfiI CCNNNNNNNGG 5 cut(s) 106, 204, 384, 646, 662
AgsI TTSAA 3 cut(s) 193, 609, 883
Alw21I GWGCWC 1 cut(s) 821
AlwNI CAGNNNCTG 2 cut(s) 533, 701
Aor13HI TCCGGA 1 cut(s) 729
AoxI GGCC 9 cut(s) 9, 81, 126, 286, 318, 375, 465, 637, 944
ApeKI GCWGC 5 cut(s) 222, 740, 755, 794, 797
ApoI RAATTY 1 cut(s) 589
ArsI GACNNNNNNTTYG 2 cut(s) 852, 884
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 2 cut(s) 32, 66
AspS9I GGNCC 1 cut(s) 9
AsuHPI GGTGA 3 cut(s) 340, 551, 728
BalI TGGCCA 1 cut(s) 320
BanI GGYRCC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 821
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 5 cut(s) 234, 727, 767, 781, 809
BccI CCATC 8 cut(s) 20, 100, 232, 325, 359, 530, 625, 914
BceAI ACGGC 2 cut(s) 40, 274
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 297, 795
BfoI RGCGCY 2 cut(s) 33, 67
BisI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
BlsI GCNGC 9 cut(s) 29, 68, 85, 224, 227, 742, 757, 796, 799
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 31
BmsI GCATC 1 cut(s) 400
BpmI CTGGAG 2 cut(s) 86, 194
Bpu10I CCTNAGC 2 cut(s) 60, 372
BpuEI CTTGAG 1 cut(s) 107
BsaHI GRCGYC 3 cut(s) 30, 111, 147
BsaJI CCNNGG 2 cut(s) 22, 429
BsaWI WCCGGW 2 cut(s) 184, 729
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 204, 384, 646, 662
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 838
BseAI TCCGGA 1 cut(s) 729
BseDI CCNNGG 2 cut(s) 22, 429
BseGI GGATG 6 cut(s) 11, 39, 569, 754, 900, 925
BseLI CCNNNNNNNGG 5 cut(s) 106, 204, 384, 646, 662
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 3 cut(s) 51, 363, 906
BseNI ACTGG 1 cut(s) 712
BseRI GAGGAG 3 cut(s) 129, 333, 827
BseXI GCAGC 5 cut(s) 234, 727, 767, 781, 809
BsgI GTGCAG 3 cut(s) 497, 687, 714
Bsh1236I CGCG 2 cut(s) 78, 431
BshFI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
BshNI GGYRCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 821
BsiSI CCGG 5 cut(s) 33, 185, 198, 640, 730
BslFI GGGAC 2 cut(s) 662, 685
BslI CCNNNNNNNGG 5 cut(s) 106, 204, 384, 646, 662
BsmFI GGGAC 2 cut(s) 662, 685
BsnI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
Bsp1286I GDGCHC 1 cut(s) 821
Bsp13I TCCGGA 1 cut(s) 729
Bsp143I GATC 1 cut(s) 580
Bsp68I TCGCGA 1 cut(s) 78
BspANI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
BspCNI CTCAG 3 cut(s) 52, 364, 907
BspEI TCCGGA 1 cut(s) 729
BspFNI CGCG 2 cut(s) 78, 431
BspLI GGNNCC 1 cut(s) 31
BspMAI CTGCAG 1 cut(s) 799
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 22, 429
BssMI GATC 1 cut(s) 580
BssNI GRCGYC 3 cut(s) 30, 111, 147
Bst4CI ACNGT 3 cut(s) 232, 298, 559
Bst6I CTCTTC 1 cut(s) 57
BstACI GRCGYC 3 cut(s) 30, 111, 147
BstAPI GCANNNNNTGC 1 cut(s) 701
BstC8I GCNNGC 4 cut(s) 34, 71, 456, 548
BstDEI CTNAG 4 cut(s) 60, 264, 372, 915
BstDSI CCRYGG 2 cut(s) 22, 429
BstENI CCTNNNNNAGG 1 cut(s) 382
BstF5I GGATG 6 cut(s) 11, 39, 569, 754, 900, 925
BstFNI CGCG 2 cut(s) 78, 431
BstH2I RGCGCY 2 cut(s) 33, 67
BstHHI GCGC 2 cut(s) 32, 66
BstKTI GATC 1 cut(s) 583
BstMBI GATC 1 cut(s) 580
BstMWI GCNNNNNNNGC 8 cut(s) 33, 70, 75, 125, 464, 701, 837, 854
BstSFI CTRYAG 2 cut(s) 297, 795
BstUI CGCG 2 cut(s) 78, 431
BstV1I GCAGC 5 cut(s) 234, 727, 767, 781, 809
BstXI CCANNNNNNTGG 1 cut(s) 316
BsuRI GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
BtgI CCRYGG 2 cut(s) 22, 429
BtsCI GGATG 6 cut(s) 11, 39, 569, 754, 900, 925
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 94, 705
BtuMI TCGCGA 1 cut(s) 78
Cac8I GCNNGC 4 cut(s) 34, 71, 456, 548
CaiI CAGNNNCTG 2 cut(s) 533, 701
CfoI GCGC 2 cut(s) 32, 66
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 9
Cfr42I CCGCGG 1 cut(s) 432
CseI GACGC 1 cut(s) 100
Csp6I GTAC 2 cut(s) 735, 769
CviQI GTAC 2 cut(s) 735, 769
DdeI CTNAG 4 cut(s) 60, 264, 372, 915
DinI GGCGCC 1 cut(s) 31
DpnI GATC 1 cut(s) 582
DpnII GATC 1 cut(s) 580
EaeI YGGCCR 2 cut(s) 318, 637
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 3 cut(s) 45, 80, 630
Eco147I AGGCCT 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 382
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
FaiI YATR 8 cut(s) 254, 311, 348, 489, 678, 764, 804, 943
FaqI GGGAC 2 cut(s) 662, 685
FauI CCCGC 1 cut(s) 51
Fnu4HI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
FokI GGATG 6 cut(s) 18, 26, 576, 761, 907, 932
Fsp4HI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 31, 65
GluI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
GsuI CTGGAG 2 cut(s) 86, 194
HaeII RGCGCY 2 cut(s) 33, 67
HaeIII GGCC 9 cut(s) 11, 83, 128, 288, 320, 377, 467, 639, 946
HapII CCGG 5 cut(s) 33, 185, 198, 640, 730
HgaI GACGC 1 cut(s) 100
HhaI GCGC 2 cut(s) 32, 66
Hin1I GRCGYC 3 cut(s) 30, 111, 147
Hin6I GCGC 2 cut(s) 30, 64
HinP1I GCGC 2 cut(s) 30, 64
HincII GTYRAC 2 cut(s) 216, 625
HindII GTYRAC 2 cut(s) 216, 625
HindIII AAGCTT 1 cut(s) 243
HpaII CCGG 5 cut(s) 33, 185, 198, 640, 730
HphI GGTGA 3 cut(s) 340, 551, 728
Hpy166II GTNNAC 2 cut(s) 216, 625
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 5 cut(s) 77, 103, 143, 167, 730
Hpy8I GTNNAC 2 cut(s) 216, 625
Hpy99I CGWCG 4 cut(s) 116, 149, 152, 155
HpyAV CCTTC 2 cut(s) 508, 957
HpyCH4III ACNGT 3 cut(s) 232, 298, 559
HpyCH4IV ACGT 2 cut(s) 147, 674
HpyCH4V TGCA 6 cut(s) 478, 602, 668, 695, 797, 958
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 70, 75, 125, 464, 701, 837, 854
HpyF3I CTNAG 4 cut(s) 60, 264, 372, 915
HpySE526I ACGT 2 cut(s) 147, 674
Hsp92I GRCGYC 3 cut(s) 30, 111, 147
HspAI GCGC 2 cut(s) 30, 64
KasI GGCGCC 1 cut(s) 29
Kpn2I TCCGGA 1 cut(s) 729
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
KspI CCGCGG 1 cut(s) 432
Kzo9I GATC 1 cut(s) 580
LmnI GCTCC 2 cut(s) 275, 368
Lsp1109I GCAGC 5 cut(s) 234, 727, 767, 781, 809
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 147, 674
MaeIII GTNAC 1 cut(s) 868
MalI GATC 1 cut(s) 582
MbiI CCGCTC 1 cut(s) 46
MboI GATC 1 cut(s) 580
MboII GAAGA 2 cut(s) 44, 530
MhlI GDGCHC 1 cut(s) 821
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 1 cut(s) 589
MluNI TGGCCA 1 cut(s) 320
Mly113I GGCGCC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 541
Mox20I TGGCCA 1 cut(s) 320
MreI CGCCGGCG 1 cut(s) 32
MroI TCCGGA 1 cut(s) 729
MroNI GCCGGC 1 cut(s) 32
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 3 cut(s) 225, 431, 740
MspI CCGG 5 cut(s) 33, 185, 198, 640, 730
MvnI CGCG 2 cut(s) 78, 431
MwoI GCNNNNNNNGC 8 cut(s) 33, 70, 75, 125, 464, 701, 837, 854
NaeI GCCGGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 30
NdeII GATC 1 cut(s) 580
NgoMIV GCCGGC 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 868
NruI TCGCGA 1 cut(s) 78
PceI AGGCCT 1 cut(s) 377
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 419
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 9 cut(s) 29, 68, 85, 224, 227, 742, 757, 796, 799
PluTI GGCGCC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 9
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 2 cut(s) 533, 701
PsyI GACNNNGTC 1 cut(s) 149
PvuII CAGCTG 1 cut(s) 740
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 2 cut(s) 736, 770
RsaNI GTAC 2 cut(s) 735, 769
SacII CCGCGG 1 cut(s) 432
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 9 cut(s) 28, 67, 84, 223, 226, 741, 756, 795, 798
Sau3AI GATC 1 cut(s) 580
Sau96I GGNCC 1 cut(s) 9
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 2 cut(s) 297, 795
SfoI GGCGCC 1 cut(s) 31
Sfr303I CCGCGG 1 cut(s) 432
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 589
SseBI AGGCCT 1 cut(s) 377
SspDI GGCGCC 1 cut(s) 29
SspI AATATT 1 cut(s) 181
SspMI CTAG 1 cut(s) 384
StuI AGGCCT 1 cut(s) 377
TaaI ACNGT 3 cut(s) 232, 298, 559
TaiI ACGT 2 cut(s) 150, 677
TaqI TCGA 4 cut(s) 114, 144, 153, 901
TasI AATT 1 cut(s) 589
TatI WGTACW 2 cut(s) 734, 768
TauI GCSGC 4 cut(s) 30, 69, 86, 228
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 101, 712
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 5 cut(s) 222, 740, 755, 794, 797
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 1 cut(s) 412
TspRI CASTG 2 cut(s) 101, 712
Tth111I GACNNNGTC 1 cut(s) 149
XagI CCTNNNNNAGG 1 cut(s) 382
XapI RAATTY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 384
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.