FvH4_5g13570

cleavage stimulation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004638
Physical Location & Seq
Forward (+)
7678111 .. 7682402
4292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13570.t4

Sequence Viewer

Length: 1302 bp
ATGGAGAGCGGCGTAGAGCAGACTCTTCAAGATGCCAAGCTCTACAGAGAGCTCAATTCTCTCATTGTCGCCTATCTTCGCGACAATAATCTTCGCCAGGCTGCAAGTGCTGTTGCTTCAGCAACAATGACGTCACTGAATGTTGAAGTCCCGGCTAATAAGCTTGTTCAGCTGGTTGCTAAGGGCCTTGCAGTGGAGAAAGATGAGGTGGTAAGGGGGGTTTCTTCTCTTGCTAATGATTTTGGTACCTTGATACCTTCGGGAACGTCGGCTCTTCACACTGCCTCTGTTGATTTTGGTGCTGTCCAGGAAACAAAAGGCTCGTCAAAGAGTTTCCCAAAGCATGAGACACGCCATCTTTCGGAGCACAAGAATATTGCCAGATGTGCAAGGTTTAGTCCTGATGGGAAGTTTGTCGCTACTGGAAGTTCAGACACGTCAATAAAGCTATTTGAGGTTGCAAAAGTTAAGCAAATGATGCTTCCAGAGGCAAGAGATGGACCTGTGCGACCTGTCATACGTACATTCTATGACCATTTACAACCCATAAATGATTTGGATTTCCATCCTCAGAGCTCTATACTTATATCTGGGGCAAAAGATCACACAATAAAGTTCTTTGATTATTCCAAAATGACTGCAAAGAGGGCATTCAGAGTTATCCAGGATACACACAATGTGTGTTCAGTATCGTTTCATCCTTCTGGAGAGTTTCTTTTAGCTGGGACTGATCATCCAATTCCTCACTTATATGATATCAATAGCTTCCAGTGTTACCTATCTGCAAATGCCCCAGAAATTGATGTTAACGGAGCAATAAATCAGATCAGGTATTCAGCTACCGGTGGCGTGTATGTTACAGCTTCCAAAGATAGTGCTATTCGCTTATGGGATGGTGTAACTGCCAGTTGCATACGGTCCATAGTTGGTGCACATGGCACAGCAGAGGCCACCAGTGCAAATTTTACCAAGGACGAAAGGTTTGTTCTCTCGTCTGGGAAGGATTCTACTGTGAAGCTTTGGGATGTAGGCACTGGAAAATTGGTTAAACAATATATAGGAGCTGCACATACTCAATTAAGATGTCAGGCTGTTTTTAATGATACCGAGGAATTTGTACTGTCCATGGATGAACCAAGCAATGAGATTGTTGTCTGGGATGCTCTGACAGCAGAAGTAGTAGCAAGGTGGCCCTCCAATCATAATGGTGCTTTCCGCTGGCTCGAGCACTCACCAACTGAAGCGGCATTTGTTTCCTGTGGGACTGACAGATCAATTCGGTTCTGGAAGGAAAGCCTATAA

Protein Analysis

434

Amino Acids

47.47

Weight (kDa)

6.32

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 7 - 142 2.6e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 312 5e-50 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 9 - 190 1.4e-16 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 10 - 143 3e-12 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 11 - 189 1.3e-10 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 41 - 244 3.2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 51 - 122 3.4e-08 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 54 - 90 3.3e-07 WD domain, G-beta repeat
WD40_Prp19 PF24814 55 - 191 3.5e-18 Prp19 WD40 domain
WD40_Gbeta PF25391 57 - 188 1.3e-08 G protein beta WD-40 repeat protein
EIF3I PF24805 57 - 234 5.9e-11 EIF3I
Beta-prop_WDR3_2nd PF25172 57 - 180 5.7e-09 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 68 - 233 6.1e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 74 - 311 3.8e-11 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 96 - 227 5.6e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 98 - 250 1.4e-16 WDR3 first beta-propeller domain
WDR55 PF24796 103 - 233 7.8e-10 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 146 - 234 6.5e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 153 - 312 5.2e-17 THOC3 beta-propeller domain
EIF3I PF24805 154 - 255 1.6e-07 EIF3I
WD40_WDHD1_1st PF24817 154 - 312 3.2e-08 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 154 - 311 2.8e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 200 - 313 3.8e-09 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 206 - 311 5.9e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 134
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 245
AccBSI CCGCTC 1 cut(s) 9
AccII CGCG 1 cut(s) 81
AciI CCGC 3 cut(s) 9, 1216, 1244
AcsI RAATTY 2 cut(s) 961, 1112
AcuI CTGAAG 2 cut(s) 102, 1260
AcyI GRCGYC 1 cut(s) 131
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 3 cut(s) 247, 523, 1119
AfiI CCNNNNNNNGG 2 cut(s) 193, 361
AflIII ACRYGT 1 cut(s) 435
AgeI ACCGGT 1 cut(s) 842
AgsI TTSAA 2 cut(s) 29, 146
AjiI CACGTC 1 cut(s) 438
AjnI CCWGG 3 cut(s) 96, 306, 663
Alw21I GWGCWC 5 cut(s) 54, 369, 578, 934, 1230
Alw26I GTCTC 1 cut(s) 341
Alw44I GTGCAC 1 cut(s) 930
Ama87I CYCGRG 1 cut(s) 1223
AoxI GGCC 3 cut(s) 184, 948, 1190
ApaLI GTGCAC 1 cut(s) 930
ApeKI GCWGC 2 cut(s) 101, 1064
ApoI RAATTY 2 cut(s) 961, 1112
ArsI GACNNNNNNTTYG 4 cut(s) 308, 340, 965, 997
AsiGI ACCGGT 1 cut(s) 842
Asp718I GGTACC 1 cut(s) 245
AspS9I GGNCC 4 cut(s) 184, 500, 918, 1191
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 1224
AvaI CYCGRG 1 cut(s) 1223
AvaII GGWCC 2 cut(s) 500, 918
BaeGI GKGCMC 1 cut(s) 934
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 2 cut(s) 54, 578
Bbv12I GWGCWC 5 cut(s) 54, 369, 578, 934, 1230
BbvI GCAGC 2 cut(s) 88, 1051
BccI CCATC 5 cut(s) 363, 398, 491, 573, 887
BciT130I CCWGG 3 cut(s) 98, 308, 665
BciVI GTATCC 1 cut(s) 661
BclI TGATCA 1 cut(s) 730
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 43
BfuI GTATCC 1 cut(s) 661
BisI GCNGC 4 cut(s) 10, 102, 1065, 1245
BlsI GCNGC 4 cut(s) 11, 103, 1066, 1246
Bme1390I CCNGG 4 cut(s) 98, 152, 308, 665
Bme18I GGWCC 2 cut(s) 500, 918
BmeT110I CYCGRG 1 cut(s) 1223
BmgBI CACGTC 1 cut(s) 438
BmgT120I GGNCC 4 cut(s) 184, 500, 918, 1191
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 4 cut(s) 98, 152, 308, 665
BmsI GCATC 3 cut(s) 22, 468, 1150
BpmI CTGGAG 1 cut(s) 726
Bpu10I CCTNAGC 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 564
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 969, 1107, 1125
BsaWI WCCGGW 1 cut(s) 842
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 361
Bse118I RCCGGY 1 cut(s) 842
Bse1I ACTGG 5 cut(s) 427, 769, 906, 954, 1039
Bse3DI GCAATG 1 cut(s) 1147
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 3 cut(s) 98, 308, 665
BseDI CCNNGG 3 cut(s) 969, 1107, 1125
BseGI GGATG 7 cut(s) 565, 697, 733, 898, 1030, 1135, 1165
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 2 cut(s) 193, 361
BseMI GCAATG 1 cut(s) 1147
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 5 cut(s) 427, 769, 906, 954, 1039
BseSI GKGCMC 1 cut(s) 934
BseXI GCAGC 2 cut(s) 88, 1051
BseYI CCCAGC 1 cut(s) 722
BsgI GTGCAG 1 cut(s) 1050
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 3 cut(s) 186, 950, 1192
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 1 cut(s) 842
BsiHKAI GWGCWC 5 cut(s) 54, 369, 578, 934, 1230
BsiHKCI CYCGRG 1 cut(s) 1223
BsiSI CCGG 2 cut(s) 152, 843
BslFI GGGAC 3 cut(s) 134, 739, 1276
BslI CCNNNNNNNGG 2 cut(s) 193, 361
BsmAI GTCTC 1 cut(s) 341
BsmFI GGGAC 3 cut(s) 134, 739, 1276
BsmI GAATGC 1 cut(s) 650
BsnI GGCC 3 cut(s) 186, 950, 1192
BsoBI CYCGRG 1 cut(s) 1223
Bsp1286I GDGCHC 5 cut(s) 54, 369, 578, 934, 1230
Bsp143I GATC 4 cut(s) 601, 730, 825, 1271
Bsp19I CCATGG 1 cut(s) 1125
Bsp68I TCGCGA 1 cut(s) 81
BspACI CCGC 3 cut(s) 9, 1216, 1244
BspANI GGCC 3 cut(s) 186, 950, 1192
BspCNI CTCAG 1 cut(s) 583
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 247
BspQI GCTCTTC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 1147
BsrFI RCCGGY 1 cut(s) 842
BsrI ACTGG 5 cut(s) 427, 769, 906, 954, 1039
BssAI RCCGGY 1 cut(s) 842
BssECI CCNNGG 3 cut(s) 969, 1107, 1125
BssMI GATC 4 cut(s) 601, 730, 825, 1271
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 2 cut(s) 969, 1125
Bst2UI CCWGG 3 cut(s) 98, 308, 665
Bst4CI ACNGT 3 cut(s) 918, 1012, 1122
Bst6I CTCTTC 2 cut(s) 30, 279
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 478
BstBAI YACGTR 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 1220
BstDEI CTNAG 2 cut(s) 180, 570
BstDSI CCRYGG 1 cut(s) 1125
BstF5I GGATG 7 cut(s) 565, 697, 733, 898, 1030, 1135, 1165
BstFNI CGCG 1 cut(s) 81
BstKTI GATC 4 cut(s) 604, 733, 828, 1274
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 4 cut(s) 601, 730, 825, 1271
BstMWI GCNNNNNNNGC 7 cut(s) 107, 169, 386, 478, 647, 956, 1169
BstNI CCWGG 3 cut(s) 98, 308, 665
BstSCI CCNGG 4 cut(s) 96, 150, 306, 663
BstSFI CTRYAG 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 934
BstSNI TACGTA 1 cut(s) 521
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 2 cut(s) 88, 1051
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 3 cut(s) 186, 950, 1192
BtgI CCRYGG 1 cut(s) 1125
BtrI CACGTC 1 cut(s) 438
BtsCI GGATG 7 cut(s) 565, 697, 733, 898, 1030, 1135, 1165
BtsI GCAGTG 2 cut(s) 198, 279
BtsIMutI CAGTG 6 cut(s) 134, 198, 279, 776, 961, 1032
BtuMI TCGCGA 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 1220
Cfr10I RCCGGY 1 cut(s) 842
Cfr13I GGNCC 4 cut(s) 184, 500, 918, 1191
Csp6I GTAC 3 cut(s) 246, 522, 1118
CspAI ACCGGT 1 cut(s) 842
CviAII CATG 3 cut(s) 344, 935, 1126
CviQI GTAC 3 cut(s) 246, 522, 1118
DdeI CTNAG 2 cut(s) 180, 570
DpnI GATC 4 cut(s) 603, 732, 827, 1273
DpnII GATC 4 cut(s) 601, 730, 825, 1271
DraIII CACNNNGTG 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 30, 279
EarI CTCTTC 2 cut(s) 30, 279
Ecl136II GAGCTC 2 cut(s) 52, 576
Eco105I TACGTA 1 cut(s) 521
Eco130I CCWWGG 2 cut(s) 969, 1125
Eco24I GRGCYC 2 cut(s) 54, 578
Eco32I GATATC 1 cut(s) 757
Eco47I GGWCC 2 cut(s) 500, 918
Eco53kI GAGCTC 2 cut(s) 52, 576
Eco57I CTGAAG 2 cut(s) 102, 1260
Eco88I CYCGRG 1 cut(s) 1223
EcoICRI GAGCTC 2 cut(s) 52, 576
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 3 cut(s) 96, 306, 663
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 969, 1125
EcoT38I GRGCYC 2 cut(s) 54, 578
ErhI CCWWGG 2 cut(s) 969, 1125
FaeI CATG 3 cut(s) 347, 938, 1129
FaqI GGGAC 3 cut(s) 134, 739, 1276
FatI CATG 3 cut(s) 343, 934, 1125
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 4 cut(s) 10, 102, 1065, 1245
FokI GGATG 7 cut(s) 552, 684, 720, 905, 1037, 1142, 1172
FriOI GRGCYC 2 cut(s) 54, 578
Fsp4HI GCNGC 4 cut(s) 10, 102, 1065, 1245
GluI GCNGC 4 cut(s) 10, 102, 1065, 1245
GsaI CCCAGC 1 cut(s) 726
GsuI CTGGAG 1 cut(s) 726
HaeIII GGCC 3 cut(s) 186, 950, 1192
HapII CCGG 2 cut(s) 152, 843
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 3 cut(s) 347, 938, 1129
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 2 cut(s) 161, 1016
HinfI GANTC 2 cut(s) 22, 1004
HpaI GTTAAC 1 cut(s) 808
HpaII CCGG 2 cut(s) 152, 843
HphI GGTGA 1 cut(s) 1224
Hpy166II GTNNAC 2 cut(s) 808, 932
Hpy188I TCNGA 6 cut(s) 364, 433, 573, 656, 825, 1167
Hpy188III TCNNGA 7 cut(s) 29, 80, 261, 401, 485, 705, 1285
Hpy8I GTNNAC 2 cut(s) 808, 932
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 4 cut(s) 267, 711, 994, 1282
HpyCH4III ACNGT 3 cut(s) 918, 1012, 1122
HpyCH4IV ACGT 4 cut(s) 131, 266, 437, 520
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 169, 386, 478, 647, 956, 1169
HpyF3I CTNAG 2 cut(s) 180, 570
HpySE526I ACGT 4 cut(s) 131, 266, 437, 520
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 3 cut(s) 347, 938, 1129
KpnI GGTACC 1 cut(s) 249
Ksp22I TGATCA 1 cut(s) 730
KspAI GTTAAC 1 cut(s) 808
Kzo9I GATC 4 cut(s) 601, 730, 825, 1271
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 3 cut(s) 364, 812, 1061
Lsp1109I GCAGC 2 cut(s) 88, 1051
LweI GCATC 3 cut(s) 22, 468, 1150
MaeII ACGT 4 cut(s) 131, 266, 437, 520
MaeIII GTNAC 4 cut(s) 132, 773, 856, 898
MalI GATC 4 cut(s) 603, 732, 827, 1273
MbiI CCGCTC 1 cut(s) 9
MboI GATC 4 cut(s) 601, 730, 825, 1271
MboII GAAGA 5 cut(s) 17, 68, 83, 216, 266
MhlI GDGCHC 5 cut(s) 54, 369, 578, 934, 1230
MluCI AATT 8 cut(s) 55, 738, 798, 961, 1040, 1076, 1112, 1275
MlyI GAGTC 1 cut(s) 16
MseI TTAA 5 cut(s) 468, 807, 1047, 1079, 1098
MslI CAYNNNNRTG 2 cut(s) 750, 1206
MspA1I CMGCKG 2 cut(s) 172, 1218
MspI CCGG 2 cut(s) 152, 843
MspR9I CCNGG 4 cut(s) 98, 152, 308, 665
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 3 cut(s) 98, 308, 665
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 7 cut(s) 107, 169, 386, 478, 647, 956, 1169
NciI CCSGG 1 cut(s) 152
NcoI CCATGG 1 cut(s) 1125
NdeII GATC 4 cut(s) 601, 730, 825, 1271
NlaIII CATG 3 cut(s) 347, 938, 1129
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 132
NruI TCGCGA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 1223
PciSI GCTCTTC 1 cut(s) 279
PctI GAATGC 1 cut(s) 650
PfeI GAWTC 1 cut(s) 1004
PfoI TCCNGGA 2 cut(s) 306, 663
PinAI ACCGGT 1 cut(s) 842
PkrI GCNGC 4 cut(s) 11, 103, 1066, 1246
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 521
Psp124BI GAGCTC 2 cut(s) 54, 578
Psp6I CCWGG 3 cut(s) 96, 306, 663
PspFI CCCAGC 1 cut(s) 722
PspGI CCWGG 3 cut(s) 96, 306, 663
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 4 cut(s) 184, 500, 918, 1191
PspXI VCTCGAGB 1 cut(s) 1223
PvuII CAGCTG 1 cut(s) 172
RruI TCGCGA 1 cut(s) 81
RsaI GTAC 3 cut(s) 247, 523, 1119
RsaNI GTAC 3 cut(s) 246, 522, 1118
RseI CAYNNNNRTG 2 cut(s) 750, 1206
SacI GAGCTC 2 cut(s) 54, 578
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 5 cut(s) 468, 807, 1047, 1079, 1098
SatI GCNGC 4 cut(s) 10, 102, 1065, 1245
Sau3AI GATC 4 cut(s) 601, 730, 825, 1271
Sau96I GGNCC 4 cut(s) 184, 500, 918, 1191
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 4 cut(s) 98, 152, 308, 665
SduI GDGCHC 5 cut(s) 54, 369, 578, 934, 1230
SfaNI GCATC 3 cut(s) 22, 468, 1150
SfcI CTRYAG 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 1223
SinI GGWCC 2 cut(s) 500, 918
SlaI CTCGAG 1 cut(s) 1223
SmiMI CAYNNNNRTG 2 cut(s) 750, 1206
SmlI CTYRAG 1 cut(s) 1223
SmoI CTYRAG 1 cut(s) 1223
SnaBI TACGTA 1 cut(s) 521
Sse9I AATT 8 cut(s) 55, 738, 798, 961, 1040, 1076, 1112, 1275
SsiI CCGC 3 cut(s) 9, 1216, 1244
SspI AATATT 1 cut(s) 376
SstI GAGCTC 2 cut(s) 54, 578
StyD4I CCNGG 4 cut(s) 96, 150, 306, 663
StyI CCWWGG 2 cut(s) 969, 1125
TaaI ACNGT 3 cut(s) 918, 1012, 1122
TaiI ACGT 4 cut(s) 134, 269, 440, 523
TaqI TCGA 1 cut(s) 1224
TasI AATT 8 cut(s) 55, 738, 798, 961, 1040, 1076, 1112, 1275
TatI WGTACW 1 cut(s) 1117
TauI GCSGC 2 cut(s) 12, 1247
TfiI GAWTC 1 cut(s) 1004
Tru1I TTAA 5 cut(s) 468, 807, 1047, 1079, 1098
Tru9I TTAA 5 cut(s) 468, 807, 1047, 1079, 1098
TscAI CASTG 6 cut(s) 141, 198, 286, 776, 961, 1039
TseFI GTSAC 1 cut(s) 132
TseI GCWGC 2 cut(s) 101, 1064
Tsp45I GTSAC 1 cut(s) 132
TspDTI ATGAA 2 cut(s) 686, 1146
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 6 cut(s) 141, 198, 286, 776, 961, 1039
VneI GTGCAC 1 cut(s) 930
VpaK11BI GGWCC 2 cut(s) 500, 918
XapI RAATTY 2 cut(s) 961, 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XhoI CTCGAG 1 cut(s) 1223
ZraI GACGTC 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.