Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0014740

Physical Location & Seq

Genomic Coordinates Forward (+)

7815938 .. 7820608

Gene Structure 4671 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g13850.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGAGGCACAGCCAGCTTCGCTATCACCATCTTCATCATCATCTTGCAGTACAAATGATAACATTATGCTACCTTCTTTCTCTTCGGTTTGGACTGAATCCGATAAAACAGAACCACTCCAAGCTGTGAACCAGGAGCAGGCACCCATTCCCATGTCAGATATTGGATTGCTGAGATTTATGGAACTCACTGCCAATGGTGGGATGAGGTGGGAGGATGTAAAGGACCGGTTTGACCGGCTTTCCATCACCCTGGGGCAAACTGAACCGGTTATAAAATGGTCTGATTTTGGTTTGTGCATAGGGATGGAACAGTCGCAAGAGTTCGCTAATCAGCTACTAAGGGCTCTAAGGAAAAGCAAGGATCGGAATTTTGGTATACCAAAAGCTGAACTGCAGAGTTACTGGTCTCGTATGACCGACCCTTGCTTCAGTTCAAGGATGCAGATATTCTTTGACATGTGTGACAGAAACATGGAGGGAAGAATAACAGAAATAGATATCAAGCAGACTATTACACTAAGTGCTACTACAAATAAGTTATTTTTGACACCTGAGGAAGCAGAAGACTATGCTGCTTTGGTCATGGAGGTGCTTGACACTGAAAAAAGGGGCTACATTGAGCTTCCACAATTTGAGACCCTCATCAAAATGAACTTATCAAAGGCCTCTTTCTCACATAACCACTTGTCAATCAGACATGATCCCTATCACAATTTTGACCAAGTTCAAAAGCCAAATTCCAGGATAAATATTTTGTTTCGAACTTATTGGCGACGAGCATGGATAGTTTTGCTGTGGTTGATCATATGTAGTGCACTCTTCACTTGGAAATTTCTACAATATAGGCATAGAGCAGCTTTTCAAGTAATGGGGTACTGCCTATCCTCTGCCAAAGGTGCTGCTGAAACCCTCAAATTCAACATGGCTCTAATTCTCCTCCCTGTCTGTCGTAATACAATTACATGGCTGCGAAGGAACCGTTCAATCAACTCAATCATTCCATTTAATGACACTATCAACTTCCACAAGCTCATAGCAGGAGGGGTAGTGGTAGGAGTTATCCTACATGGTGGTTGTCACCTAGCCTGTGATTTACCAAGAATTAGTGAGTCAGATCATGTAATATTCGGGCAAACAATAGCTGCCCGATTCGGGTACCATCAACCATCATACTTTGAAATATTGACCACAAAGGAAGTGGCATCAGGAATTGGTATGGTGATACTAATGGCCGTAGCATTCTCACTTGCAACACGATGGCCACGCCGGGGGTCACCTTCACTGCCAAAGTCAGTCCGGAATGTCACAGGATACAATACCTTTTGGTACTCACACCACTTGTTCATTCCTGTATATGCATTGCTCATTTTTCACTCTATATTCCTCTTCCTTACAAACAACGTAACTGAAAAAACGACATTGATGTACATTGCCATTCCAGTTCTGTTATACACCGGAGAACGAGTGTTTCGAGCCATAAGATCTGGATTCTATGAGGTGGAAATGTCAAAGGCAAGTATTTATCCTGGAAAAGTTTTGTCCCTCAAATTGCACAAACCAAAAGGTTTCAAGTACCTGAGTGGCATGTACATATTCATTCAATGCCCTCAGATTTCGCCATTTGAATGGCACCCGTTTTCGTTAACTTCAGGACCAGAAGATGACTACCTAAGTGTGCATATTAGAACTACTGGGGACTGGAGTTACCAAATATACAGTCTTTTCCAAGAGGCAACATTATCAGGGCTTAACAGTTACCCTAAAGTACACATTGATGGACCTTATGGTGCTCCATCTCAAGACCATATCAAGTATGATATCATTGTTCTAATCGGTCTAGGCATAGGCGCTACACCTTTCATTGGCATCCTCAAAGATATTGTCAATAGTGCTCAAAACTCATCACATTGTGATCATCATTCTGGTTGCAGAGAATGCAGCTCAAGAAAGTCTCCACTAAAAGCCTATCTTTTTTGGGTCACAAGAGAACAGAGCTCCTTTGAATGGTTTAGAGACATCATGAAGGAAATATCAGATTCAAAAGAAAAGCAGTCTATTGTAGAGATGCACAGTTTCTTAACTTCTGTTTATCAAGAGGGAGATGCGCGTTCGGTTCTGATAAGTGCAATACAAGCATTGCTTTATGCCAAAAATGGTATTGACTTTGTCTCCCGGACTCCGGTAAGAACACATTTTGCCCGGCCAAACTGGTCCAGCATCTTCTCAGAATTGGCTTGTAGACACAGAGGAGCACGGATTGGGGTTTTCTACTGTGGGGCTTCAACCTTGGCAGAAGAGTTGCAGGGAATGTGTACCAAATTTTCGACCAAAACTTCCACAAGATTTGTATTTCACAAGGAGAATTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0001101 GO:0002252 GO:0002376 GO:0002679 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0010941 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0016999 GO:0017000 GO:0017144 GO:0023052 GO:0032870 GO:0033036 GO:0033037 GO:0033500 GO:0033554 GO:0033993 GO:0035556 GO:0042221 GO:0042592 GO:0042743 GO:0043067 GO:0043069 GO:0044237 GO:0044249 GO:0044464 GO:0045730 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050665 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051716 GO:0052542 GO:0052545 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071396 GO:0071470 GO:0071495 GO:0071944 GO:0072593 GO:0097305 GO:0097306 GO:0104004 GO:1901700 GO:1901701 GO:1903409

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

791

Amino Acids

90.07

Weight (kDa)

8.74

Isoelectric Point (pI)

50.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	58 - 146	7.3e-18	Respiratory burst NADPH oxidase
Ferric_reduct	PF01794	309 - 456	2e-09	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	499 - 600	2.4e-28	FAD-binding domain
NAD_binding_6	PF08030	606 - 728	2.6e-22	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014740)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g13850 FvH4_5g13850 FvH4_5g13850
malus_domestica	MD14G1211700.v1.1
prunus_persica	Prupe.5G204900_v2.0.a1 Prupe.5G204900_v2.0.a1
pyrus_communis	pycom14g17530
rosa_chinensis	RchiOBHm_Chr7g0183081
rosa_laevigata	RLG00000005080
rosa_multiflora	Rmu_ssc0000111.1_g000005
rosa_roxburghii	Rroxscaffold_3G00270780
rosa_rugosa	Rorug06G0452600
rosa_samantha	Rh7AG055900 Rh7BG055200 Rh7CG056700 Rh7DG055400
rosa_wichuraiana	Rw7G004550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	275
Acc65I	GGTACC	1 cut(s)	1158
AccB1I	GGYRCC	3 cut(s)	142, 1158, 1632
AccI	GTMKAC	2 cut(s)	379, 2242
AccII	CGCG	1 cut(s)	2110
AccIII	TCCGGA	1 cut(s)	1299
AclWI	GGATC	2 cut(s)	372, 698
AcoI	YGGCCR	3 cut(s)	1233, 1262, 2204
AcsI	RAATTY	5 cut(s)	370, 739, 833, 917, 2321
AcuI	CTGAAG	2 cut(s)	415, 1635
AdeI	CACNNNGTG	2 cut(s)	524, 1913
AfaI	GTAC	9 cut(s)	52, 878, 1160, 1331, 1430, 1577, 1592, 1770, 2317
AfiI	CCNNNNNNNGG	7 cut(s)	139, 201, 1155, 1271, 1865, 2007, 2182
AflIII	ACRYGT	1 cut(s)	459
AgeI	ACCGGT	2 cut(s)	228, 268
AjnI	CCWGG	4 cut(s)	132, 252, 743, 1528
Alw21I	GWGCWC	5 cut(s)	820, 1795, 1897, 2000, 2257
Alw26I	GTCTC	5 cut(s)	414, 632, 1959, 2010, 2176
Alw44I	GTGCAC	1 cut(s)	816
AlwI	GGATC	2 cut(s)	372, 698
Aor13HI	TCCGGA	1 cut(s)	1299
AoxI	GGCC	4 cut(s)	666, 1233, 1262, 2204
ApaLI	GTGCAC	1 cut(s)	816
ApeKI	GCWGC	6 cut(s)	575, 857, 902, 970, 1145, 1941
ApoI	RAATTY	5 cut(s)	370, 739, 833, 917, 2321
ArsI	GACNNNNNNTTYG	4 cut(s)	1705, 1737, 1869, 1901
AsiGI	ACCGGT	2 cut(s)	228, 268
Asp718I	GGTACC	1 cut(s)	1158
AspLEI	GCGC	2 cut(s)	1853, 2110
AspS9I	GGNCC	4 cut(s)	226, 1655, 1781, 2214
AsuC2I	CCSGG	3 cut(s)	1271, 2176, 2203
AsuHPI	GGTGA	5 cut(s)	18, 241, 1073, 1234, 1269
AsuII	TTCGAA	1 cut(s)	763
AvaII	GGWCC	4 cut(s)	226, 1655, 1781, 2214
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	1 cut(s)	820
BalI	TGGCCA	1 cut(s)	1264
BanI	GGYRCC	3 cut(s)	142, 1158, 1632
BanII	GRGCYC	2 cut(s)	349, 2000
BbsI	GAAGAC	1 cut(s)	573
Bbv12I	GWGCWC	5 cut(s)	820, 1795, 1897, 2000, 2257
BbvI	GCAGC	6 cut(s)	562, 869, 889, 957, 1132, 1953
BccI	CCATC	8 cut(s)	37, 254, 301, 1170, 1177, 1254, 1772, 1804
BceAI	ACGGC	1 cut(s)	1220
BciT130I	CCWGG	4 cut(s)	134, 254, 745, 1530
BciVI	GTATCC	1 cut(s)	1307
BclI	TGATCA	2 cut(s)	804, 1915
BcnI	CCSGG	3 cut(s)	1271, 2176, 2203
BcoDI	GTCTC	5 cut(s)	414, 632, 1959, 2010, 2176
BfaI	CTAG	2 cut(s)	1085, 1841
BfmI	CTRYAG	1 cut(s)	395
BfoI	RGCGCY	1 cut(s)	1854
BfuI	GTATCC	1 cut(s)	1307
BglII	AGATCT	1 cut(s)	1484
BisI	GCNGC	6 cut(s)	576, 858, 903, 971, 1146, 1942
BlsI	GCNGC	6 cut(s)	577, 859, 904, 972, 1147, 1943
Bme1390I	CCNGG	7 cut(s)	134, 254, 745, 1271, 1530, 2176, 2203
Bme18I	GGWCC	4 cut(s)	226, 1655, 1781, 2214
BmgT120I	GGNCC	4 cut(s)	226, 1655, 1781, 2214
BmiI	GGNNCC	4 cut(s)	144, 980, 1160, 1634
BmrFI	CCNGG	7 cut(s)	134, 254, 745, 1271, 1530, 2176, 2203
BmrI	ACTGGG	1 cut(s)	1704
BmsI	GCATC	6 cut(s)	432, 1214, 1878, 2058, 2095, 2229
BmuI	ACTGGG	1 cut(s)	1704
BpiI	GAAGAC	1 cut(s)	573
BpmI	CTGGAG	1 cut(s)	1723
Bpu14I	TTCGAA	1 cut(s)	763
BpuEI	CTTGAG	2 cut(s)	1785, 1930
BpuMI	CCSGG	3 cut(s)	1271, 2176, 2203
BsaBI	GATNNNNATC	1 cut(s)	708
BsaI	GGTCTC	2 cut(s)	414, 632
BsaJI	CCNNGG	4 cut(s)	252, 253, 1270, 2289
BsaWI	WCCGGW	5 cut(s)	228, 268, 1299, 1457, 2182
BsaXI	ACNNNNNCTCC	6 cut(s)	1035, 1065, 1452, 1482, 2156, 2186
Bsc4I	CCNNNNNNNGG	7 cut(s)	139, 201, 1155, 1271, 1865, 2007, 2182
Bse118I	RCCGGY	3 cut(s)	228, 237, 268
Bse1I	ACTGG	5 cut(s)	410, 1442, 1699, 1706, 2216
Bse21I	CCTNAGG	1 cut(s)	555
Bse3DI	GCAATG	3 cut(s)	1361, 1431, 2138
Bse8I	GATNNNNATC	1 cut(s)	708
BseAI	TCCGGA	1 cut(s)	1299
BseBI	CCWGG	4 cut(s)	134, 254, 745, 1530
BseDI	CCNNGG	4 cut(s)	252, 253, 1270, 2289
BseGI	GGATG	5 cut(s)	210, 223, 312, 447, 1869
BseJI	GATNNNNATC	1 cut(s)	708
BseLI	CCNNNNNNNGG	7 cut(s)	139, 201, 1155, 1271, 1865, 2007, 2182
BseMI	GCAATG	3 cut(s)	1361, 1431, 2138
BseMII	CTCAG	5 cut(s)	164, 546, 1571, 1625, 2241
BseNI	ACTGG	5 cut(s)	410, 1442, 1699, 1706, 2216
BseRI	GAGGAG	2 cut(s)	929, 2265
BseSI	GKGCMC	1 cut(s)	820
BseXI	GCAGC	6 cut(s)	562, 869, 889, 957, 1132, 1953
Bsh1236I	CGCG	1 cut(s)	2110
BshFI	GGCC	4 cut(s)	668, 1235, 1264, 2206
BshNI	GGYRCC	3 cut(s)	142, 1158, 1632
BshTI	ACCGGT	2 cut(s)	228, 268
BsiHKAI	GWGCWC	5 cut(s)	820, 1795, 1897, 2000, 2257
BsiSI	CCGG	9 cut(s)	229, 238, 269, 1270, 1300, 1458, 2176, 2183, 2203
BslFI	GGGAC	2 cut(s)	1528, 1712
BslI	CCNNNNNNNGG	7 cut(s)	139, 201, 1155, 1271, 1865, 2007, 2182
BsmAI	GTCTC	5 cut(s)	414, 632, 1959, 2010, 2176
BsmFI	GGGAC	2 cut(s)	1528, 1712
BsmI	GAATGC	2 cut(s)	1241, 1943
BsnI	GGCC	4 cut(s)	668, 1235, 1264, 2206
Bso31I	GGTCTC	2 cut(s)	414, 632
Bsp119I	TTCGAA	1 cut(s)	763
Bsp1286I	GDGCHC	6 cut(s)	349, 820, 1795, 1897, 2000, 2257
Bsp13I	TCCGGA	1 cut(s)	1299
Bsp1407I	TGTACA	2 cut(s)	1428, 1590
Bsp143I	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
BspANI	GGCC	4 cut(s)	668, 1235, 1264, 2206
BspCNI	CTCAG	5 cut(s)	165, 547, 1572, 1624, 2240
BspEI	TCCGGA	1 cut(s)	1299
BspFNI	CGCG	1 cut(s)	2110
BspHI	TCATGA	1 cut(s)	2022
BspLI	GGNNCC	4 cut(s)	144, 980, 1160, 1634
BspMAI	CTGCAG	1 cut(s)	399
BspPI	GGATC	2 cut(s)	372, 698
BspT104I	TTCGAA	1 cut(s)	763
BspT107I	GGYRCC	3 cut(s)	142, 1158, 1632
BspTNI	GGTCTC	2 cut(s)	414, 632
BsrDI	GCAATG	3 cut(s)	1361, 1431, 2138
BsrFI	RCCGGY	3 cut(s)	228, 237, 268
BsrGI	TGTACA	2 cut(s)	1428, 1590
BsrI	ACTGG	5 cut(s)	410, 1442, 1699, 1706, 2216
BssAI	RCCGGY	3 cut(s)	228, 237, 268
BssECI	CCNNGG	4 cut(s)	252, 253, 1270, 2289
BssMI	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
BssNAI	GTATAC	1 cut(s)	380
BssT1I	CCWWGG	1 cut(s)	2289
Bst1107I	GTATAC	1 cut(s)	380
Bst2UI	CCWGG	4 cut(s)	134, 254, 745, 1530
Bst4CI	ACNGT	6 cut(s)	315, 983, 1721, 1757, 2075, 2276
Bst6I	CTCTTC	4 cut(s)	88, 827, 1394, 2292
BstAPI	GCANNNNNTGC	1 cut(s)	1938
BstAUI	TGTACA	2 cut(s)	1428, 1590
BstBI	TTCGAA	1 cut(s)	763
BstC8I	GCNNGC	2 cut(s)	15, 141
BstDEI	CTNAG	9 cut(s)	173, 341, 350, 521, 555, 1580, 1611, 1673, 2227
BstEII	GGTNACC	1 cut(s)	1275
BstF5I	GGATG	5 cut(s)	210, 223, 312, 447, 1869
BstFNI	CGCG	1 cut(s)	2110
BstH2I	RGCGCY	1 cut(s)	1854
BstHHI	GCGC	2 cut(s)	1853, 2110
BstKTI	GATC	6 cut(s)	367, 706, 807, 1120, 1487, 1918
BstMAI	GTCTC	5 cut(s)	414, 632, 1959, 2010, 2176
BstMBI	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
BstMWI	GCNNNNNNNGC	5 cut(s)	14, 19, 899, 1938, 2135
BstNI	CCWGG	4 cut(s)	134, 254, 745, 1530
BstNSI	RCATGY	2 cut(s)	463, 1591
BstPI	GGTNACC	1 cut(s)	1275
BstSCI	CCNGG	7 cut(s)	132, 252, 743, 1269, 1528, 2174, 2201
BstSFI	CTRYAG	1 cut(s)	395
BstSLI	GKGCMC	1 cut(s)	820
BstUI	CGCG	1 cut(s)	2110
BstV1I	GCAGC	6 cut(s)	562, 869, 889, 957, 1132, 1953
BstV2I	GAAGAC	1 cut(s)	573
BstX2I	RGATCY	1 cut(s)	1484
BstXI	CCANNNNNNTGG	2 cut(s)	253, 1629
BstYI	RGATCY	1 cut(s)	1484
BstZ17I	GTATAC	1 cut(s)	380
Bsu36I	CCTNAGG	1 cut(s)	555
BsuI	GTATCC	1 cut(s)	1307
BsuRI	GGCC	4 cut(s)	668, 1235, 1264, 2206
BtsCI	GGATG	5 cut(s)	210, 223, 312, 447, 1869
BtsI	GCAGTG	2 cut(s)	189, 1283
BtsIMutI	CAGTG	3 cut(s)	189, 600, 1283
Cac8I	GCNNGC	2 cut(s)	15, 141
CciI	TCATGA	1 cut(s)	2022
CfoI	GCGC	2 cut(s)	1853, 2110
Cfr10I	RCCGGY	3 cut(s)	228, 237, 268
Cfr13I	GGNCC	4 cut(s)	226, 1655, 1781, 2214
Csp6I	GTAC	9 cut(s)	51, 877, 1159, 1330, 1429, 1576, 1591, 1769, 2316
CspAI	ACCGGT	2 cut(s)	228, 268
CspCI	CAANNNNNGTGG	4 cut(s)	1182, 1217, 2329, 2364
CviQI	GTAC	9 cut(s)	51, 877, 1159, 1330, 1429, 1576, 1591, 1769, 2316
DdeI	CTNAG	9 cut(s)	173, 341, 350, 521, 555, 1580, 1611, 1673, 2227
DpnI	GATC	6 cut(s)	366, 705, 806, 1119, 1486, 1917
DpnII	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
DraIII	CACNNNGTG	2 cut(s)	524, 1913
EaeI	YGGCCR	3 cut(s)	1233, 1262, 2204
Eam1104I	CTCTTC	4 cut(s)	88, 827, 1394, 2292
EarI	CTCTTC	4 cut(s)	88, 827, 1394, 2292
Ecl136II	GAGCTC	1 cut(s)	1998
Eco130I	CCWWGG	1 cut(s)	2289
Eco147I	AGGCCT	1 cut(s)	668
Eco24I	GRGCYC	2 cut(s)	349, 2000
Eco31I	GGTCTC	2 cut(s)	414, 632
Eco32I	GATATC	2 cut(s)	502, 1822
Eco47I	GGWCC	4 cut(s)	226, 1655, 1781, 2214
Eco53kI	GAGCTC	1 cut(s)	1998
Eco57I	CTGAAG	2 cut(s)	415, 1635
Eco81I	CCTNAGG	1 cut(s)	555
Eco91I	GGTNACC	1 cut(s)	1275
EcoICRI	GAGCTC	1 cut(s)	1998
EcoO65I	GGTNACC	1 cut(s)	1275
EcoRII	CCWGG	4 cut(s)	132, 252, 743, 1528
EcoRV	GATATC	2 cut(s)	502, 1822
EcoT14I	CCWWGG	1 cut(s)	2289
EcoT22I	ATGCAT	1 cut(s)	1363
EcoT38I	GRGCYC	2 cut(s)	349, 2000
ErhI	CCWWGG	1 cut(s)	2289
FalI	AAGNNNNNCTT	4 cut(s)	1956, 1988, 2127, 2159
FaqI	GGGAC	2 cut(s)	1528, 1712
FauNDI	CATATG	1 cut(s)	809
FbaI	TGATCA	2 cut(s)	804, 1915
FblI	GTMKAC	2 cut(s)	379, 2242
Fnu4HI	GCNGC	6 cut(s)	576, 858, 903, 971, 1146, 1942
FokI	GGATG	5 cut(s)	217, 230, 319, 454, 1856
FriOI	GRGCYC	2 cut(s)	349, 2000
Fsp4HI	GCNGC	6 cut(s)	576, 858, 903, 971, 1146, 1942
FspBI	CTAG	2 cut(s)	1085, 1841
GlaI	GCGC	2 cut(s)	1852, 2109
GluI	GCNGC	6 cut(s)	576, 858, 903, 971, 1146, 1942
GsuI	CTGGAG	1 cut(s)	1723
HaeII	RGCGCY	1 cut(s)	1854
HaeIII	GGCC	4 cut(s)	668, 1235, 1264, 2206
HapII	CCGG	9 cut(s)	229, 238, 269, 1270, 1300, 1458, 2176, 2183, 2203
HhaI	GCGC	2 cut(s)	1853, 2110
Hin6I	GCGC	2 cut(s)	1851, 2108
HinP1I	GCGC	2 cut(s)	1851, 2108
HincII	GTYRAC	1 cut(s)	1647
HindII	GTYRAC	1 cut(s)	1647
HinfI	GANTC	6 cut(s)	98, 1112, 1152, 1491, 2039, 2179
HpaI	GTTAAC	1 cut(s)	1647
HpaII	CCGG	9 cut(s)	229, 238, 269, 1270, 1300, 1458, 2176, 2183, 2203
HphI	GGTGA	5 cut(s)	18, 241, 1073, 1234, 1269
Hpy166II	GTNNAC	7 cut(s)	130, 380, 818, 1647, 1771, 2243, 2316
Hpy188III	TCNNGA	8 cut(s)	1209, 1300, 1488, 1653, 1802, 1947, 2023, 2096
Hpy8I	GTNNAC	7 cut(s)	130, 380, 818, 1647, 1771, 2243, 2316
Hpy99I	CGWCG	1 cut(s)	780
HpyAV	CCTTC	4 cut(s)	84, 969, 1290, 2020
HpyCH4III	ACNGT	6 cut(s)	315, 983, 1721, 1757, 2075, 2276
HpyCH4IV	ACGT	1 cut(s)	1404
HpyF10VI	GCNNNNNNNGC	5 cut(s)	14, 19, 899, 1938, 2135
HpyF3I	CTNAG	9 cut(s)	173, 341, 350, 521, 555, 1580, 1611, 1673, 2227
HpySE526I	ACGT	1 cut(s)	1404
HspAI	GCGC	2 cut(s)	1851, 2108
Kpn2I	TCCGGA	1 cut(s)	1299
KpnI	GGTACC	1 cut(s)	1162
Ksp22I	TGATCA	2 cut(s)	804, 1915
KspAI	GTTAAC	1 cut(s)	1647
Kzo9I	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
LmnI	GCTCC	4 cut(s)	136, 1798, 2003, 2252
Lsp1109I	GCAGC	6 cut(s)	562, 869, 889, 957, 1132, 1953
LweI	GCATC	6 cut(s)	432, 1214, 1878, 2058, 2095, 2229
MaeI	CTAG	2 cut(s)	1085, 1841
MaeII	ACGT	1 cut(s)	1404
MaeIII	GTNAC	9 cut(s)	401, 464, 1079, 1275, 1306, 1405, 1706, 1757, 1981
MalI	GATC	6 cut(s)	366, 705, 806, 1119, 1486, 1917
MboI	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
MboII	GAAGA	9 cut(s)	24, 75, 495, 578, 814, 1381, 1673, 2215, 2309
MflI	RGATCY	1 cut(s)	1484
MhlI	GDGCHC	6 cut(s)	349, 820, 1795, 1897, 2000, 2257
MlsI	TGGCCA	1 cut(s)	1264
MluNI	TGGCCA	1 cut(s)	1264
MlyI	GAGTC	2 cut(s)	1121, 2173
Mox20I	TGGCCA	1 cut(s)	1264
Mph1103I	ATGCAT	1 cut(s)	1363
MroI	TCCGGA	1 cut(s)	1299
MscI	TGGCCA	1 cut(s)	1264
MseI	TTAA	5 cut(s)	1008, 1646, 1752, 2081, 2371
MslI	CAYNNNNRTG	6 cut(s)	152, 305, 590, 650, 1627, 1776
Msp20I	TGGCCA	1 cut(s)	1264
MspI	CCGG	9 cut(s)	229, 238, 269, 1270, 1300, 1458, 2176, 2183, 2203
MspR9I	CCNGG	7 cut(s)	134, 254, 745, 1271, 1530, 2176, 2203
Mva1269I	GAATGC	2 cut(s)	1241, 1943
MvaI	CCWGG	4 cut(s)	134, 254, 745, 1530
MvnI	CGCG	1 cut(s)	2110
MwoI	GCNNNNNNNGC	5 cut(s)	14, 19, 899, 1938, 2135
NciI	CCSGG	3 cut(s)	1271, 2176, 2203
NdeI	CATATG	1 cut(s)	809
NdeII	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
NlaIV	GGNNCC	4 cut(s)	144, 980, 1160, 1634
NmuCI	GTSAC	5 cut(s)	464, 1079, 1275, 1306, 1981
NsiI	ATGCAT	1 cut(s)	1363
NspI	RCATGY	2 cut(s)	463, 1591
NspV	TTCGAA	1 cut(s)	763
PagI	TCATGA	1 cut(s)	2022
PasI	CCCWGGG	1 cut(s)	253
PceI	AGGCCT	1 cut(s)	668
PciI	ACATGT	1 cut(s)	459
PcsI	WCGNNNNNNNCGW	1 cut(s)	1471
PctI	GAATGC	2 cut(s)	1241, 1943
PfeI	GAWTC	4 cut(s)	98, 1152, 1491, 2039
PflFI	GACNNNGTC	1 cut(s)	2168
PfoI	TCCNGGA	3 cut(s)	743, 1528, 2174
PinAI	ACCGGT	2 cut(s)	228, 268
PkrI	GCNGC	6 cut(s)	577, 859, 904, 972, 1147, 1943
PleI	GAGTC	2 cut(s)	1120, 2173
PpsI	GAGTC	2 cut(s)	1120, 2173
PscI	ACATGT	1 cut(s)	459
PsiI	TTATAA	1 cut(s)	275
Psp124BI	GAGCTC	1 cut(s)	2000
Psp6I	CCWGG	4 cut(s)	132, 252, 743, 1528
PspEI	GGTNACC	1 cut(s)	1275
PspGI	CCWGG	4 cut(s)	132, 252, 743, 1528
PspN4I	GGNNCC	4 cut(s)	144, 980, 1160, 1634
PspPI	GGNCC	4 cut(s)	226, 1655, 1781, 2214
PstI	CTGCAG	1 cut(s)	399
PsuI	RGATCY	1 cut(s)	1484
PsyI	GACNNNGTC	1 cut(s)	2168
RsaI	GTAC	9 cut(s)	52, 878, 1160, 1331, 1430, 1577, 1592, 1770, 2317
RsaNI	GTAC	9 cut(s)	51, 877, 1159, 1330, 1429, 1576, 1591, 1769, 2316
RseI	CAYNNNNRTG	6 cut(s)	152, 305, 590, 650, 1627, 1776
SacI	GAGCTC	1 cut(s)	2000
SaqAI	TTAA	5 cut(s)	1008, 1646, 1752, 2081, 2371
SatI	GCNGC	6 cut(s)	576, 858, 903, 971, 1146, 1942
Sau3AI	GATC	6 cut(s)	364, 703, 804, 1117, 1484, 1915
Sau96I	GGNCC	4 cut(s)	226, 1655, 1781, 2214
SchI	GAGTC	2 cut(s)	1121, 2173
ScrFI	CCNGG	7 cut(s)	134, 254, 745, 1271, 1530, 2176, 2203
SduI	GDGCHC	6 cut(s)	349, 820, 1795, 1897, 2000, 2257
SfaNI	GCATC	6 cut(s)	432, 1214, 1878, 2058, 2095, 2229
SfcI	CTRYAG	1 cut(s)	395
SfuI	TTCGAA	1 cut(s)	763
SinI	GGWCC	4 cut(s)	226, 1655, 1781, 2214
SmiMI	CAYNNNNRTG	6 cut(s)	152, 305, 590, 650, 1627, 1776
SmlI	CTYRAG	2 cut(s)	1800, 1945
SmoI	CTYRAG	2 cut(s)	1800, 1945
SseBI	AGGCCT	1 cut(s)	668
SspI	AATATT	3 cut(s)	754, 1128, 1185
SspMI	CTAG	2 cut(s)	1085, 1841
SstI	GAGCTC	1 cut(s)	2000
StuI	AGGCCT	1 cut(s)	668
StyD4I	CCNGG	7 cut(s)	132, 252, 743, 1269, 1528, 2174, 2201
StyI	CCWWGG	1 cut(s)	2289
TaaI	ACNGT	6 cut(s)	315, 983, 1721, 1757, 2075, 2276
TaiI	ACGT	1 cut(s)	1407
TaqI	TCGA	3 cut(s)	763, 1474, 2327
TaqII	GACCGA	2 cut(s)	434, 1826
TatI	WGTACW	4 cut(s)	50, 1428, 1590, 1768
TfiI	GAWTC	4 cut(s)	98, 1152, 1491, 2039
Tru1I	TTAA	5 cut(s)	1008, 1646, 1752, 2081, 2371
Tru9I	TTAA	5 cut(s)	1008, 1646, 1752, 2081, 2371
TscAI	CASTG	3 cut(s)	196, 607, 1290
TseFI	GTSAC	5 cut(s)	464, 1079, 1275, 1306, 1981
TseI	GCWGC	6 cut(s)	575, 857, 902, 970, 1145, 1941
Tsp45I	GTSAC	5 cut(s)	464, 1079, 1275, 1306, 1981
TspDTI	ATGAA	6 cut(s)	24, 668, 1336, 1588, 1852, 2039
TspGWI	ACGGA	1 cut(s)	2272
TspRI	CASTG	3 cut(s)	196, 607, 1290
Tth111I	GACNNNGTC	1 cut(s)	2168
VneI	GTGCAC	1 cut(s)	816
VpaK11BI	GGWCC	4 cut(s)	226, 1655, 1781, 2214
XapI	RAATTY	5 cut(s)	370, 739, 833, 917, 2321
XceI	RCATGY	2 cut(s)	463, 1591
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1198
XmiI	GTMKAC	2 cut(s)	379, 2242
XspI	CTAG	2 cut(s)	1085, 1841
Zsp2I	ATGCAT	1 cut(s)	1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.