Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0014740

Physical Location & Seq

Genomic Coordinates Forward (+)

29690183 .. 29695068

Gene Structure 4886 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1211700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGGAGGCACATGTACAGCTTCAAACTGCTCACCAACCCTATGTTTCAGCTATGTCACCGTCTTCATCTTCATCATCTTCTTCATCTTCTTGCAGTGCTAGTGTTTTCCCTCCGTCTTTCTCTCCGATTTGGACTGAACCGCACGATGAAACTGGAGTTCTGAACCGGCAGCAACATTATATGGCAGAAAATGGGGAGGCATCGTCCACAGCCGGGTCAGCCAATGAGATTGAATGGCTAAGATTTATTGACATCACAGCCAACGGTGGGATGAAGTGGAAGGATGTAGAGGAGCAGTTCGACCGGCTTGCATTGACCAGACACGGCGTTGAACCGGCTATAAAATCGTCGGATTTTGGTCTGTGCATAGGGATGCAACAGTCGCCGGAGTTTGCTAATGAGCTATTAAGGACTCTAAGAAGAAGAATGGATCGAAATTTCGATATGACAAAATGCGAACTGTATGGTTACTGGTGTCTAATGACTGACCCTCGCTTCGATTCAAGGATACGAATTTACTTTGACTTGTGTGACAAAAACATGGATGGAAGAGTGACTGAGAAAGACATTAAGCAGATCATAACACTAAGTGCTTCTACGAATAAGTTGTCTTTAACTCATGAGGAAGCAGAAGACTATGCTGCATTAGTCATGAAATTGCTTGACACCGAAAACCGGAGTTACCTTGAGTTCCCTCAGTTTAGGACTCTCTTCAATGTGATGAGCTTATCAAAAAGCTCATTTTCAACTAATCACTTTTCAATCAGACTTAGTCGTCTACATCACAATTTTGATCCAACTGAAGAGCCAAGGTCTAAAACTGAAATTTTATTTCGATCATACTGGCGGCGTGCATGGATAGTTTTGCTTTGGTTGATCATTTGCAGTGCGCTTTTCGCTTGGAAATTCACCCAGTATCGCCGTAAAGCAGCTTTTCAAGTAATGGGTTACTGCTTATCCACTGCTAAAGGTGCTGCAGAAACCCTGAAGTTCAACATGGCTCTGATTCTCCTTCCTGTTTGCCGTAATACAATCACGTGGCTTCGCAAGAACCGTTCTATAAACTCCATCATTCCGTTTAATGACAACATAAACTTCCACAAAATGATTGCAGGAGGGATAGTAGTAGGAGTAATCCTACATGGAGGTTCTCACCTTGCTTGTGATTTTCCAAGAATCAGCGACTCGGATCGTATGGTCTTCCGGCAAACAATAGCTGCCCGATTCGGGTACCATCAGCCATCATACTGTGAAATATTGGCTACAAAGGAGGTGGCAACAGGGATTGCAATGGTAGTACTAATGGCCATAGCATTTTCTCTTGCAACAAAATGGCCACGACGGCAGTCGCCTTCGCTGCCAAAGTCTGTCCGGAATATCACAGGCTACAACGCCTTCTGGTACTCACACCACCTGTTCATTCCTGTGTATGCATTGCTCATTCTCCACTCCATGTTCCTCTTCCTTACCAACAAAGCAACCGAAAAAACGACATGGATGTACATTGCCGTTCCAGCTGTATTATATGCTGGAGAACGAGTTTTCCGAGCCATAAGATCGGGGTTTTATGAGGTGGAGATTTTGAAGGCAAGCATTTATCCAGGAAAAGTTTTGTCCCTCAAATTGCAGAAACCAGAAGGTTTTCGGTACCTGAGTGGCATGTACATATTCATCCAATGCCCTCAAATTTCACCGTTTGAATGGCACCCATTTTCCTTAACTTCGGGGCCAGAAGATGACTACCTAAGCGTTCACATTCGAACTGCTGGAGACTGGAGTTATCAGATATACAGTCTTTTCCAAGAGGCAACTTTATCTGGAGTCGATGGCTATCCGAAGGTACACATCGATGGACCTTATGGTGCTGCCTCTCAAGACCATGTCAAGTATGACATCGTTGTTCTCATCGGTCTAGGCATAGGCGCTACGCCTTTCGTTAGCATCCTTAAAGACGTGGTCAATGCTGCTAAAAAACCACATTGTGATCATAGTGGTTGCAGAGAATGTAGCTCAAGAAAGGCTCCATTGAAAGCCTATCTTTATTGGGTTACAAGAGAACAAAGCTCCTTTGATTGGTTTAGAGACATCATGAAAGAAGTCTCAATCTCAAACCAAAAGCAGTCTCTTGTGGAGATGCACAACTTCTTAACATGTGTGTACCCGGAGGGGGATGCGCGATCGGTTCTAATAAGCACTATACAAGCGTTGCATCACGCGAAAAATGGTACCGATTTTCTCTCCCGGACTCCGGTACGCACACATTTCGGTCGACCGGACTGGTTCAACATCTTCTCAAAATTAGCAAGGGGACACAAAGAAGAACGGATCGGTGAGAATGCATGTAAATTCTCAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0001101 GO:0002252 GO:0002376 GO:0002679 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0010941 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0016999 GO:0017000 GO:0017144 GO:0023052 GO:0032870 GO:0033036 GO:0033037 GO:0033500 GO:0033554 GO:0033993 GO:0035556 GO:0042221 GO:0042592 GO:0042743 GO:0043067 GO:0043069 GO:0044237 GO:0044249 GO:0044464 GO:0045730 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050665 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051716 GO:0052542 GO:0052545 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071396 GO:0071470 GO:0071495 GO:0071944 GO:0072593 GO:0097305 GO:0097306 GO:0104004 GO:1901700 GO:1901701 GO:1903409

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

786

Amino Acids

89.5

Weight (kDa)

8.84

Isoelectric Point (pI)

52.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	72 - 168	1.4e-19	Respiratory burst NADPH oxidase
Ferric_reduct	PF01794	333 - 480	4.5e-10	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	523 - 624	7e-27	FAD-binding domain
NAD_binding_6	PF08030	630 - 781	2.9e-31	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014740)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g13850 FvH4_5g13850 FvH4_5g13850
malus_domestica	MD14G1211700.v1.1
prunus_persica	Prupe.5G204900_v2.0.a1 Prupe.5G204900_v2.0.a1
pyrus_communis	pycom14g17530
rosa_chinensis	RchiOBHm_Chr7g0183081
rosa_laevigata	RLG00000005080
rosa_multiflora	Rmu_ssc0000111.1_g000005
rosa_roxburghii	Rroxscaffold_3G00270780
rosa_rugosa	Rorug06G0452600
rosa_samantha	Rh7AG055900 Rh7BG055200 Rh7CG056700 Rh7DG055400
rosa_wichuraiana	Rw7G004550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	774
Acc65I	GGTACC	3 cut(s)	1230, 1647, 2225
AccB1I	GGYRCC	4 cut(s)	1230, 1647, 1704, 2225
AccI	GTMKAC	2 cut(s)	778, 2269
AccII	CGCG	2 cut(s)	2176, 2216
AccIII	TCCGGA	1 cut(s)	1371
AciI	CCGC	2 cut(s)	140, 847
AclWI	GGATC	4 cut(s)	438, 788, 1197, 2333
AcoI	YGGCCR	2 cut(s)	1305, 1334
AcsI	RAATTY	6 cut(s)	436, 513, 825, 905, 1686, 2345
AcuI	CTGAAG	2 cut(s)	822, 1007
AcvI	CACGTG	1 cut(s)	1038
AdeI	CACNNNGTG	2 cut(s)	590, 1982
AfiI	CCNNNNNNNGG	7 cut(s)	213, 267, 675, 1227, 2073, 2167, 2248
AflIII	ACRYGT	2 cut(s)	10, 2150
AjiI	CACGTC	1 cut(s)	1954
AjnI	CCWGG	1 cut(s)	1600
AloI	GAACNNNNNNTCC	2 cut(s)	1527, 1559
Alw26I	GTCTC	4 cut(s)	1764, 2076, 2104, 2127
AlwI	GGATC	4 cut(s)	438, 788, 1197, 2333
Aor13HI	TCCGGA	1 cut(s)	1371
AoxI	GGCC	3 cut(s)	1305, 1334, 1727
ApeKI	GCWGC	8 cut(s)	169, 641, 929, 974, 1217, 1357, 1865, 1964
ApoI	RAATTY	6 cut(s)	436, 513, 825, 905, 1686, 2345
ArsI	GACNNNNNNTTYG	4 cut(s)	479, 511, 593, 625
Asp700I	GAANNNNTTC	1 cut(s)	1641
Asp718I	GGTACC	3 cut(s)	1230, 1647, 2225
AspLEI	GCGC	3 cut(s)	892, 1925, 2176
AspS9I	GGNCC	2 cut(s)	1727, 1853
AsuC2I	CCSGG	3 cut(s)	214, 2162, 2242
AsuHPI	GGTGA	6 cut(s)	23, 48, 901, 1145, 1683, 2342
AsuII	TTCGAA	1 cut(s)	1759
AvaII	GGWCC	1 cut(s)	1853
BaeI	ACNNNNGTAYC	2 cut(s)	1639, 1672
BalI	TGGCCA	2 cut(s)	1307, 1336
BanI	GGYRCC	4 cut(s)	1230, 1647, 1704, 2225
BbrPI	CACGTG	1 cut(s)	1038
BbsI	GAAGAC	3 cut(s)	54, 639, 1192
BbvI	GCAGC	8 cut(s)	181, 628, 941, 961, 1204, 1344, 1852, 1951
BccI	CCATC	6 cut(s)	539, 1076, 1242, 1249, 1820, 1844
BceAI	ACGGC	5 cut(s)	340, 906, 1008, 1358, 1493
BcgI	CGANNNNNNTGC	4 cut(s)	290, 324, 2245, 2279
BciT130I	CCWGG	1 cut(s)	1602
BciVI	GTATCC	1 cut(s)	501
BclI	TGATCA	2 cut(s)	876, 1984
BcnI	CCSGG	3 cut(s)	214, 2162, 2242
BcoDI	GTCTC	4 cut(s)	1764, 2076, 2104, 2127
BfaI	CTAG	2 cut(s)	99, 1913
BfmI	CTRYAG	1 cut(s)	975
BfoI	RGCGCY	1 cut(s)	1926
BfuI	GTATCC	1 cut(s)	501
BglI	GCCNNNNNGGC	1 cut(s)	1342
BisI	GCNGC	9 cut(s)	170, 642, 848, 930, 975, 1218, 1358, 1866, 1965
BlsI	GCNGC	9 cut(s)	171, 643, 849, 931, 976, 1219, 1359, 1867, 1966
BmcAI	AGTACT	1 cut(s)	1299
Bme1390I	CCNGG	4 cut(s)	214, 1602, 2162, 2242
Bme18I	GGWCC	1 cut(s)	1853
BmgBI	CACGTC	1 cut(s)	1954
BmgT120I	GGNCC	2 cut(s)	1727, 1853
BmiI	GGNNCC	6 cut(s)	1232, 1649, 1706, 1728, 2022, 2227
BmrFI	CCNGG	4 cut(s)	214, 1602, 2162, 2242
BmrI	ACTGGG	1 cut(s)	907
BmsI	GCATC	6 cut(s)	209, 363, 1950, 2124, 2161, 2218
BmuI	ACTGGG	1 cut(s)	907
BoxI	GACNNNNGTC	1 cut(s)	1345
BpiI	GAAGAC	3 cut(s)	54, 639, 1192
BpmI	CTGGAG	5 cut(s)	174, 1551, 1788, 1795, 1839
Bpu10I	CCTNAGC	1 cut(s)	1745
Bpu14I	TTCGAA	1 cut(s)	1759
BpuEI	CTTGAG	3 cut(s)	707, 1857, 1996
BpuMI	CCSGG	3 cut(s)	214, 2162, 2242
Bsa29I	ATCGAT	1 cut(s)	1848
BsaAI	YACGTR	1 cut(s)	1038
BsaBI	GATNNNNATC	1 cut(s)	1830
BsaJI	CCNNGG	1 cut(s)	809
BsaWI	WCCGGW	4 cut(s)	675, 1371, 2248, 2272
BsaXI	ACNNNNNCTCC	6 cut(s)	188, 218, 1107, 1137, 1524, 1554
Bsc4I	CCNNNNNNNGG	7 cut(s)	213, 267, 675, 1227, 2073, 2167, 2248
Bse118I	RCCGGY	3 cut(s)	165, 303, 334
Bse1I	ACTGG	6 cut(s)	157, 476, 848, 913, 1778, 2282
Bse3DI	GCAATG	3 cut(s)	1296, 1433, 1503
Bse8I	GATNNNNATC	1 cut(s)	1830
BseAI	TCCGGA	1 cut(s)	1371
BseBI	CCWGG	1 cut(s)	1602
BseCI	ATCGAT	1 cut(s)	1848
BseDI	CCNNGG	1 cut(s)	809
BseGI	GGATG	8 cut(s)	276, 289, 378, 550, 1503, 1671, 1941, 2176
BseJI	GATNNNNATC	1 cut(s)	1830
BseLI	CCNNNNNNNGG	7 cut(s)	213, 267, 675, 1227, 2073, 2167, 2248
BseMI	GCAATG	3 cut(s)	1296, 1433, 1503
BseMII	CTCAG	3 cut(s)	549, 710, 1643
BseNI	ACTGG	6 cut(s)	157, 476, 848, 913, 1778, 2282
BseRI	GAGGAG	1 cut(s)	305
BseXI	GCAGC	8 cut(s)	181, 628, 941, 961, 1204, 1344, 1852, 1951
Bsh1236I	CGCG	2 cut(s)	2176, 2216
Bsh1285I	CGRYCG	4 cut(s)	304, 2180, 2269, 2273
BshFI	GGCC	3 cut(s)	1307, 1336, 1729
BshNI	GGYRCC	4 cut(s)	1230, 1647, 1704, 2225
BshVI	ATCGAT	1 cut(s)	1848
BsiEI	CGRYCG	4 cut(s)	304, 2180, 2269, 2273
BslFI	GGGAC	2 cut(s)	1600, 2322
BslI	CCNNNNNNNGG	7 cut(s)	213, 267, 675, 1227, 2073, 2167, 2248
BsmAI	GTCTC	4 cut(s)	1764, 2076, 2104, 2127
BsmFI	GGGAC	2 cut(s)	1600, 2322
BsmI	GAATGC	1 cut(s)	2341
BsnI	GGCC	3 cut(s)	1307, 1336, 1729
Bsp119I	TTCGAA	1 cut(s)	1759
Bsp13I	TCCGGA	1 cut(s)	1371
Bsp1407I	TGTACA	3 cut(s)	13, 1500, 1662
BspACI	CCGC	2 cut(s)	140, 847
BspANI	GGCC	3 cut(s)	1307, 1336, 1729
BspCNI	CTCAG	3 cut(s)	550, 709, 1644
BspDI	ATCGAT	1 cut(s)	1848
BspEI	TCCGGA	1 cut(s)	1371
BspFNI	CGCG	2 cut(s)	2176, 2216
BspHI	TCATGA	3 cut(s)	619, 651, 2088
BspLI	GGNNCC	6 cut(s)	1232, 1649, 1706, 1728, 2022, 2227
BspMAI	CTGCAG	1 cut(s)	979
BspPI	GGATC	4 cut(s)	438, 788, 1197, 2333
BspQI	GCTCTTC	1 cut(s)	798
BspT104I	TTCGAA	1 cut(s)	1759
BspT107I	GGYRCC	4 cut(s)	1230, 1647, 1704, 2225
BsrDI	GCAATG	3 cut(s)	1296, 1433, 1503
BsrFI	RCCGGY	3 cut(s)	165, 303, 334
BsrGI	TGTACA	3 cut(s)	13, 1500, 1662
BsrI	ACTGG	6 cut(s)	157, 476, 848, 913, 1778, 2282
BssAI	RCCGGY	3 cut(s)	165, 303, 334
BssECI	CCNNGG	1 cut(s)	809
BssT1I	CCWWGG	1 cut(s)	809
Bst2UI	CCWGG	1 cut(s)	1602
Bst4CI	ACNGT	8 cut(s)	60, 266, 381, 462, 1055, 1250, 1695, 1793
Bst6I	CTCTTC	4 cut(s)	544, 716, 798, 1466
BstAUI	TGTACA	3 cut(s)	13, 1500, 1662
BstBAI	YACGTR	1 cut(s)	1038
BstBI	TTCGAA	1 cut(s)	1759
BstC8I	GCNNGC	3 cut(s)	309, 852, 1591
BstDEI	CTNAG	9 cut(s)	239, 416, 558, 587, 696, 770, 1652, 1745, 2350
BstF5I	GGATG	8 cut(s)	276, 289, 378, 550, 1503, 1671, 1941, 2176
BstFNI	CGCG	2 cut(s)	2176, 2216
BstH2I	RGCGCY	1 cut(s)	1926
BstHHI	GCGC	3 cut(s)	892, 1925, 2176
BstMAI	GTCTC	4 cut(s)	1764, 2076, 2104, 2127
BstMCI	CGRYCG	4 cut(s)	304, 2180, 2269, 2273
BstMWI	GCNNNNNNNGC	7 cut(s)	218, 382, 896, 971, 1342, 1357, 1514
BstNI	CCWGG	1 cut(s)	1602
BstNSI	RCATGY	4 cut(s)	14, 1663, 2154, 2343
BstPAI	GACNNNNGTC	1 cut(s)	1345
BstSCI	CCNGG	4 cut(s)	212, 1600, 2160, 2240
BstSFI	CTRYAG	1 cut(s)	975
BstUI	CGCG	2 cut(s)	2176, 2216
BstV1I	GCAGC	8 cut(s)	181, 628, 941, 961, 1204, 1344, 1852, 1951
BstV2I	GAAGAC	3 cut(s)	54, 639, 1192
Bsu15I	ATCGAT	1 cut(s)	1848
BsuI	GTATCC	1 cut(s)	501
BsuRI	GGCC	3 cut(s)	1307, 1336, 1729
BsuTUI	ATCGAT	1 cut(s)	1848
BtrI	CACGTC	1 cut(s)	1954
BtsCI	GGATG	8 cut(s)	276, 289, 378, 550, 1503, 1671, 1941, 2176
BtsI	GCAGTG	3 cut(s)	100, 892, 960
BtsIMutI	CAGTG	3 cut(s)	100, 892, 960
Cac8I	GCNNGC	3 cut(s)	309, 852, 1591
CciI	TCATGA	3 cut(s)	619, 651, 2088
CfoI	GCGC	3 cut(s)	892, 1925, 2176
Cfr10I	RCCGGY	3 cut(s)	165, 303, 334
Cfr13I	GGNCC	2 cut(s)	1727, 1853
ClaI	ATCGAT	1 cut(s)	1848
CspCI	CAANNNNNGTGG	2 cut(s)	1254, 1289
DdeI	CTNAG	9 cut(s)	239, 416, 558, 587, 696, 770, 1652, 1745, 2350
DraIII	CACNNNGTG	2 cut(s)	590, 1982
DrdI	GACNNNNNNGTC	1 cut(s)	774
DseDI	GACNNNNNNGTC	1 cut(s)	774
EaeI	YGGCCR	2 cut(s)	1305, 1334
Eam1104I	CTCTTC	4 cut(s)	544, 716, 798, 1466
EarI	CTCTTC	4 cut(s)	544, 716, 798, 1466
Eco130I	CCWWGG	1 cut(s)	809
Eco47I	GGWCC	1 cut(s)	1853
Eco57I	CTGAAG	2 cut(s)	822, 1007
Eco72I	CACGTG	1 cut(s)	1038
EcoRII	CCWGG	1 cut(s)	1600
EcoT14I	CCWWGG	1 cut(s)	809
EcoT22I	ATGCAT	2 cut(s)	1435, 2341
ErhI	CCWWGG	1 cut(s)	809
FalI	AAGNNNNNCTT	2 cut(s)	2022, 2054
FaqI	GGGAC	2 cut(s)	1600, 2322
FbaI	TGATCA	2 cut(s)	876, 1984
FblI	GTMKAC	2 cut(s)	778, 2269
Fnu4HI	GCNGC	9 cut(s)	170, 642, 848, 930, 975, 1218, 1358, 1866, 1965
FokI	GGATG	8 cut(s)	283, 296, 385, 557, 1510, 1658, 1928, 2183
Fsp4HI	GCNGC	9 cut(s)	170, 642, 848, 930, 975, 1218, 1358, 1866, 1965
FspBI	CTAG	2 cut(s)	99, 1913
GlaI	GCGC	3 cut(s)	891, 1924, 2175
GluI	GCNGC	9 cut(s)	170, 642, 848, 930, 975, 1218, 1358, 1866, 1965
GsuI	CTGGAG	5 cut(s)	174, 1551, 1788, 1795, 1839
HaeII	RGCGCY	1 cut(s)	1926
HaeIII	GGCC	3 cut(s)	1307, 1336, 1729
HhaI	GCGC	3 cut(s)	892, 1925, 2176
Hin6I	GCGC	3 cut(s)	890, 1923, 2174
HinP1I	GCGC	3 cut(s)	890, 1923, 2174
HincII	GTYRAC	1 cut(s)	2270
HindII	GTYRAC	1 cut(s)	2270
HinfI	GANTC	9 cut(s)	412, 500, 706, 1006, 1176, 1184, 1224, 1821, 2245
HphI	GGTGA	6 cut(s)	23, 48, 901, 1145, 1683, 2342
Hpy166II	GTNNAC	6 cut(s)	207, 779, 1753, 1843, 2158, 2270
Hpy188I	TCNGA	9 cut(s)	126, 162, 352, 767, 1005, 1189, 1547, 1785, 1836
Hpy188III	TCNNGA	7 cut(s)	620, 652, 1372, 1818, 1874, 2013, 2089
Hpy8I	GTNNAC	6 cut(s)	207, 779, 1753, 1843, 2158, 2270
Hpy99I	CGWCG	2 cut(s)	352, 1344
HpyAV	CCTTC	7 cut(s)	274, 1022, 1362, 1405, 1579, 1631, 1831
HpyCH4III	ACNGT	8 cut(s)	60, 266, 381, 462, 1055, 1250, 1695, 1793
HpyCH4IV	ACGT	2 cut(s)	1037, 1953
HpyF10VI	GCNNNNNNNGC	7 cut(s)	218, 382, 896, 971, 1342, 1357, 1514
HpyF3I	CTNAG	9 cut(s)	239, 416, 558, 587, 696, 770, 1652, 1745, 2350
HpySE526I	ACGT	2 cut(s)	1037, 1953
HspAI	GCGC	3 cut(s)	890, 1923, 2174
Kpn2I	TCCGGA	1 cut(s)	1371
KpnI	GGTACC	3 cut(s)	1234, 1651, 2229
Ksp22I	TGATCA	2 cut(s)	876, 1984
LguI	GCTCTTC	1 cut(s)	798
LmnI	GCTCC	3 cut(s)	292, 2026, 2069
Lsp1109I	GCAGC	8 cut(s)	181, 628, 941, 961, 1204, 1344, 1852, 1951
LweI	GCATC	6 cut(s)	209, 363, 1950, 2124, 2161, 2218
MaeI	CTAG	2 cut(s)	99, 1913
MaeII	ACGT	2 cut(s)	1037, 1953
MaeIII	GTNAC	7 cut(s)	54, 467, 530, 553, 680, 947, 2047
MlsI	TGGCCA	2 cut(s)	1307, 1336
MluNI	TGGCCA	2 cut(s)	1307, 1336
MlyI	GAGTC	5 cut(s)	406, 700, 1178, 1830, 2239
MmeI	TCCRAC	2 cut(s)	330, 821
Mox20I	TGGCCA	2 cut(s)	1307, 1336
Mph1103I	ATGCAT	2 cut(s)	1435, 2341
MroI	TCCGGA	1 cut(s)	1371
MroXI	GAANNNNTTC	1 cut(s)	1641
MscI	TGGCCA	2 cut(s)	1307, 1336
MseI	TTAA	7 cut(s)	407, 570, 614, 1080, 1718, 1947, 2147
MslI	CAYNNNNRTG	3 cut(s)	371, 1424, 1848
Msp20I	TGGCCA	2 cut(s)	1307, 1336
MspA1I	CMGCKG	1 cut(s)	1517
MspR9I	CCNGG	4 cut(s)	214, 1602, 2162, 2242
Mva1269I	GAATGC	1 cut(s)	2341
MvaI	CCWGG	1 cut(s)	1602
MvnI	CGCG	2 cut(s)	2176, 2216
MwoI	GCNNNNNNNGC	7 cut(s)	218, 382, 896, 971, 1342, 1357, 1514
NciI	CCSGG	3 cut(s)	214, 2162, 2242
NlaIV	GGNNCC	6 cut(s)	1232, 1649, 1706, 1728, 2022, 2227
NmuCI	GTSAC	3 cut(s)	54, 530, 553
NsiI	ATGCAT	2 cut(s)	1435, 2341
NspI	RCATGY	4 cut(s)	14, 1663, 2154, 2343
NspV	TTCGAA	1 cut(s)	1759
PagI	TCATGA	3 cut(s)	619, 651, 2088
PciI	ACATGT	2 cut(s)	10, 2150
PciSI	GCTCTTC	1 cut(s)	798
PcsI	WCGNNNNNNNCGW	2 cut(s)	1051, 1543
PctI	GAATGC	1 cut(s)	2341
PdmI	GAANNNNTTC	1 cut(s)	1641
PfeI	GAWTC	4 cut(s)	500, 1006, 1176, 1224
PflFI	GACNNNGTC	3 cut(s)	771, 1880, 1955
PfoI	TCCNGGA	2 cut(s)	1600, 2240
PkrI	GCNGC	9 cut(s)	171, 643, 849, 931, 976, 1219, 1359, 1867, 1966
Ple19I	CGATCG	1 cut(s)	2180
PleI	GAGTC	5 cut(s)	406, 700, 1178, 1829, 2239
PmaCI	CACGTG	1 cut(s)	1038
PmlI	CACGTG	1 cut(s)	1038
PpsI	GAGTC	5 cut(s)	406, 700, 1178, 1829, 2239
Ppu21I	YACGTR	1 cut(s)	1038
PscI	ACATGT	2 cut(s)	10, 2150
PshAI	GACNNNNGTC	1 cut(s)	1345
Psp6I	CCWGG	1 cut(s)	1600
PspCI	CACGTG	1 cut(s)	1038
PspGI	CCWGG	1 cut(s)	1600
PspN4I	GGNNCC	6 cut(s)	1232, 1649, 1706, 1728, 2022, 2227
PspPI	GGNCC	2 cut(s)	1727, 1853
PstI	CTGCAG	1 cut(s)	979
PsyI	GACNNNGTC	3 cut(s)	771, 1880, 1955
PvuI	CGATCG	1 cut(s)	2180
PvuII	CAGCTG	1 cut(s)	1517
RseI	CAYNNNNRTG	3 cut(s)	371, 1424, 1848
SalI	GTCGAC	1 cut(s)	2268
SapI	GCTCTTC	1 cut(s)	798
SaqAI	TTAA	7 cut(s)	407, 570, 614, 1080, 1718, 1947, 2147
SatI	GCNGC	9 cut(s)	170, 642, 848, 930, 975, 1218, 1358, 1866, 1965
Sau96I	GGNCC	2 cut(s)	1727, 1853
ScaI	AGTACT	1 cut(s)	1299
SchI	GAGTC	5 cut(s)	406, 700, 1178, 1830, 2239
ScrFI	CCNGG	4 cut(s)	214, 1602, 2162, 2242
SfaNI	GCATC	6 cut(s)	209, 363, 1950, 2124, 2161, 2218
SfcI	CTRYAG	1 cut(s)	975
SfuI	TTCGAA	1 cut(s)	1759
SinI	GGWCC	1 cut(s)	1853
SmiMI	CAYNNNNRTG	3 cut(s)	371, 1424, 1848
SmlI	CTYRAG	3 cut(s)	686, 1872, 2011
SmoI	CTYRAG	3 cut(s)	686, 1872, 2011
SsiI	CCGC	2 cut(s)	140, 847
SspI	AATATT	1 cut(s)	1257
SspMI	CTAG	2 cut(s)	99, 1913
StyD4I	CCNGG	4 cut(s)	212, 1600, 2160, 2240
StyI	CCWWGG	1 cut(s)	809
TaaI	ACNGT	8 cut(s)	60, 266, 381, 462, 1055, 1250, 1695, 1793
TaiI	ACGT	2 cut(s)	1040, 1956
TaqI	TCGA	9 cut(s)	300, 433, 441, 498, 835, 1759, 1824, 1848, 2269
TaqII	GACCGA	2 cut(s)	1898, 2255
TatI	WGTACW	4 cut(s)	13, 1297, 1500, 1662
TauI	GCSGC	1 cut(s)	850
TfiI	GAWTC	4 cut(s)	500, 1006, 1176, 1224
Tru1I	TTAA	7 cut(s)	407, 570, 614, 1080, 1718, 1947, 2147
Tru9I	TTAA	7 cut(s)	407, 570, 614, 1080, 1718, 1947, 2147
TscAI	CASTG	3 cut(s)	100, 892, 967
TseFI	GTSAC	3 cut(s)	54, 530, 553
TseI	GCWGC	8 cut(s)	169, 641, 929, 974, 1217, 1357, 1865, 1964
Tsp45I	GTSAC	3 cut(s)	54, 530, 553
TspDTI	ATGAA	9 cut(s)	54, 60, 72, 162, 287, 668, 1408, 1660, 2105
TspGWI	ACGGA	3 cut(s)	102, 1065, 2338
TspRI	CASTG	3 cut(s)	100, 892, 967
Tth111I	GACNNNGTC	3 cut(s)	771, 1880, 1955
VpaK11BI	GGWCC	1 cut(s)	1853
XapI	RAATTY	6 cut(s)	436, 513, 825, 905, 1686, 2345
XceI	RCATGY	4 cut(s)	14, 1663, 2154, 2343
XmiI	GTMKAC	2 cut(s)	778, 2269
XmnI	GAANNNNTTC	1 cut(s)	1641
XspI	CTAG	2 cut(s)	99, 1913
ZrmI	AGTACT	1 cut(s)	1299
Zsp2I	ATGCAT	2 cut(s)	1435, 2341

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.