FvH4_5g15990

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015090
Physical Location & Seq
Reverse (-)
9077302 .. 9079530
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15990.t1

Sequence Viewer

Length: 858 bp
ATGCCAACGAGGACACGTACATACATGTCAGGAAGGTTGTTGGTGGCGCTGATGACCCAACCGTGCACATTAGCAGCGCCACCTCCACTTGCCTCCTCTATCCTCCGCCGCCCACCACCTTCATTCTCCCAACGCTTCATCGCAACAATGACCACCAACAACCAAGACCACCTCGACGCTTTGTTCAAGCAAAAACGGATGCTCCGAACCAAAGTCCGGAAGGCCCTCAAGTCAATGGACCCAGTACTCAGATCCCAGGAAGATGATGCGATTCAGAGCATTGTGTTGGAAGCTCCTTGGTTTAAGTCTAGTCAGAGATTGTGTGCGTATATAAGCTGTGCAGCTTTGCGTGAAGTTGATACTTCTAAAGTGTTGGCTGAGATTCTTCAGGGTTCGGATAAAGATGGTGTGAGGAAAAAGCTTTATGTGCCGCGTGTGGAGGACAAGAATAGTCACATGAGAATGCTCCACATCTCAAGTGTCGATGATATGGTTGCGAATTCGATGGATATTTTAGAACCGGCTGCAGTGGATGCTGATGGAAATGAACGTGAAGATGTTTTACAGGCAAGTGATCCAGTTGATTTGCTTCTCTTACCAGGACTAGCATTTGACAGATCTGGGAGACGTTTGGGTCGAGGTGGAGGTTATTATGATACTTTTCTGAGGAATTACCAAGAGCTTGTGAAGGCCCGCAATTGGAAGCAACCGCTCCTGGTTGCACTATCGTATTCTCCACAGATAGTGGATGAAGGGGTTATAGTGGTCACCGAACATGATGTTTCAGTTGATGCTCTTGTGTCCCCAGCCGGTGTGATTCCGATCAGTACGACTGCCATAGACAGGATGAAACTTTGA

Protein Analysis

286

Amino Acids

31.74

Weight (kDa)

8.7

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 65 - 269 5.9e-33 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13050 AT5G13050
fragaria_vesca FvH4_5g15990
malus_domestica MD06G1184000.v1.1
prunus_persica Prupe.5G182700_v2.0.a1
pyrus_communis pycom06g16300
rosa_chinensis RchiOBHm_Chr7g0180081
rosa_laevigata RLG00000005296
rosa_multiflora Rmu_sc0017616.1_g000003 Rmu_ssc0000119.1_g000029
rosa_roxburghii Rroxscaffold_3G00273130
rosa_rugosa Rorug06G0430400
rosa_samantha Rh7AG030700 Rh7CG032400 Rh7DG031400
rosa_wichuraiana Rw7G002560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 633
AccBSI CCGCTC 1 cut(s) 712
AccII CGCG 1 cut(s) 433
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 5 cut(s) 106, 109, 431, 694, 710
AclWI GGATC 2 cut(s) 246, 569
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 3 cut(s) 19, 246, 829
AfiI CCNNNNNNNGG 3 cut(s) 216, 699, 843
AflIII ACRYGT 2 cut(s) 14, 24
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 3 cut(s) 255, 598, 714
AluBI AGCT 5 cut(s) 293, 336, 344, 421, 682
AluI AGCT 5 cut(s) 293, 336, 344, 421, 682
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 1 cut(s) 619
Alw44I GTGCAC 1 cut(s) 64
AlwI GGATC 2 cut(s) 246, 569
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 2 cut(s) 222, 690
ApaLI GTGCAC 1 cut(s) 64
ApeKI GCWGC 3 cut(s) 74, 341, 524
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 2 cut(s) 49, 79
AspS9I GGNCC 3 cut(s) 223, 238, 691
AsuHPI GGTGA 1 cut(s) 760
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 68
BarI GAAGNNNNNNTAC 2 cut(s) 546, 578
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 86, 353, 511
BccI CCATC 3 cut(s) 398, 499, 533
BciT130I CCWGG 3 cut(s) 257, 600, 716
BcoDI GTCTC 1 cut(s) 619
BfaI CTAG 2 cut(s) 309, 605
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 2 cut(s) 50, 80
BglII AGATCT 1 cut(s) 617
BisI GCNGC 5 cut(s) 75, 109, 342, 431, 525
BlsI GCNGC 5 cut(s) 76, 110, 343, 432, 526
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 3 cut(s) 257, 600, 716
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 3 cut(s) 223, 238, 691
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 3 cut(s) 257, 600, 716
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 4 cut(s) 189, 256, 523, 781
BmuI ACTGGG 1 cut(s) 236
BpuEI CTTGAG 2 cut(s) 212, 460
BsaAI YACGTR 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 821
BsaJI CCNNGG 2 cut(s) 255, 296
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 699, 843
Bse118I RCCGGY 2 cut(s) 520, 809
Bse1I ACTGG 2 cut(s) 242, 578
Bse8I GATNNNNATC 1 cut(s) 821
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 3 cut(s) 257, 600, 716
BseDI CCNNGG 2 cut(s) 255, 296
BseGI GGATG 4 cut(s) 204, 538, 754, 852
BseJI GATNNNNATC 1 cut(s) 821
BseLI CCNNNNNNNGG 3 cut(s) 216, 699, 843
BseMII CTCAG 3 cut(s) 262, 369, 656
BseNI ACTGG 2 cut(s) 242, 578
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 3 cut(s) 86, 353, 511
BseYI CCCAGC 1 cut(s) 805
BsgI GTGCAG 1 cut(s) 360
Bsh1236I CGCG 1 cut(s) 433
BshFI GGCC 2 cut(s) 224, 692
BsiHKAI GWGCWC 1 cut(s) 68
BsiSI CCGG 3 cut(s) 217, 521, 810
BslFI GGGAC 1 cut(s) 787
BslI CCNNNNNNNGG 3 cut(s) 216, 699, 843
BsmAI GTCTC 1 cut(s) 619
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 1 cut(s) 787
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 2 cut(s) 224, 692
Bsp1286I GDGCHC 1 cut(s) 68
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 4 cut(s) 251, 574, 617, 822
BspACI CCGC 5 cut(s) 106, 109, 431, 694, 710
BspANI GGCC 2 cut(s) 224, 692
BspCNI CTCAG 3 cut(s) 261, 370, 657
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 1 cut(s) 433
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 2 cut(s) 246, 569
BsrBI CCGCTC 1 cut(s) 712
BsrFI RCCGGY 2 cut(s) 520, 809
BsrI ACTGG 2 cut(s) 242, 578
BssAI RCCGGY 2 cut(s) 520, 809
BssECI CCNNGG 2 cut(s) 255, 296
BssMI GATC 4 cut(s) 251, 574, 617, 822
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 3 cut(s) 257, 600, 716
Bst4CI ACNGT 1 cut(s) 63
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 694
BstDEI CTNAG 3 cut(s) 248, 378, 665
BstEII GGTNACC 1 cut(s) 766
BstF5I GGATG 4 cut(s) 204, 538, 754, 852
BstFNI CGCG 1 cut(s) 433
BstH2I RGCGCY 2 cut(s) 50, 80
BstHHI GCGC 2 cut(s) 49, 79
BstKTI GATC 4 cut(s) 254, 577, 620, 825
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 4 cut(s) 251, 574, 617, 822
BstMWI GCNNNNNNNGC 2 cut(s) 427, 533
BstNI CCWGG 3 cut(s) 257, 600, 716
BstNSI RCATGY 1 cut(s) 28
BstPI GGTNACC 1 cut(s) 766
BstSCI CCNGG 3 cut(s) 255, 598, 714
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 3 cut(s) 86, 353, 511
BstX2I RGATCY 2 cut(s) 251, 617
BstYI RGATCY 2 cut(s) 251, 617
BsuRI GGCC 2 cut(s) 224, 692
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 4 cut(s) 204, 538, 754, 852
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 694
CfoI GCGC 2 cut(s) 49, 79
Cfr10I RCCGGY 2 cut(s) 520, 809
Cfr13I GGNCC 3 cut(s) 223, 238, 691
CseI GACGC 1 cut(s) 185
Csp6I GTAC 3 cut(s) 18, 245, 828
CviAII CATG 3 cut(s) 25, 457, 776
CviQI GTAC 3 cut(s) 18, 245, 828
DdeI CTNAG 3 cut(s) 248, 378, 665
DpnI GATC 4 cut(s) 253, 576, 619, 824
DpnII GATC 4 cut(s) 251, 574, 617, 822
DrdI GACNNNNNNGTC 1 cut(s) 633
DseDI GACNNNNNNGTC 1 cut(s) 633
EciI GGCGGA 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 371
Eco91I GGTNACC 1 cut(s) 766
EcoO109I RGGNCCY 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 766
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 255, 598, 714
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 3 cut(s) 28, 460, 779
FaqI GGGAC 1 cut(s) 787
FatI CATG 3 cut(s) 24, 456, 775
FauI CCCGC 1 cut(s) 701
Fnu4HI GCNGC 5 cut(s) 75, 109, 342, 431, 525
FokI GGATG 3 cut(s) 211, 545, 761
Fsp4HI GCNGC 5 cut(s) 75, 109, 342, 431, 525
FspBI CTAG 2 cut(s) 309, 605
GlaI GCGC 2 cut(s) 48, 78
GluI GCNGC 5 cut(s) 75, 109, 342, 431, 525
GsaI CCCAGC 1 cut(s) 809
HaeII RGCGCY 2 cut(s) 50, 80
HaeIII GGCC 2 cut(s) 224, 692
HapII CCGG 3 cut(s) 217, 521, 810
HgaI GACGC 1 cut(s) 185
HhaI GCGC 2 cut(s) 49, 79
Hin1II CATG 3 cut(s) 28, 460, 779
Hin6I GCGC 2 cut(s) 47, 77
HinP1I GCGC 2 cut(s) 47, 77
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 3 cut(s) 271, 382, 817
HpaII CCGG 3 cut(s) 217, 521, 810
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 7 cut(s) 206, 251, 276, 315, 397, 666, 822
Hpy188III TCNNGA 2 cut(s) 30, 217
Hpy8I GTNNAC 1 cut(s) 66
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 5 cut(s) 27, 129, 214, 682, 746
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 3 cut(s) 16, 550, 628
HpyCH4V TGCA 4 cut(s) 66, 341, 527, 722
HpyF10VI GCNNNNNNNGC 2 cut(s) 427, 533
HpyF3I CTNAG 3 cut(s) 248, 378, 665
HpySE526I ACGT 3 cut(s) 16, 550, 628
Hsp92II CATG 3 cut(s) 28, 460, 779
HspAI GCGC 2 cut(s) 47, 77
Kpn2I TCCGGA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 251, 574, 617, 822
LmnI GCTCC 4 cut(s) 207, 298, 471, 717
Lsp1109I GCAGC 3 cut(s) 86, 353, 511
LweI GCATC 4 cut(s) 189, 256, 523, 781
MaeI CTAG 2 cut(s) 309, 605
MaeII ACGT 3 cut(s) 16, 550, 628
MaeIII GTNAC 2 cut(s) 452, 766
MalI GATC 4 cut(s) 253, 576, 619, 824
MbiI CCGCTC 1 cut(s) 712
MboI GATC 4 cut(s) 251, 574, 617, 822
MboII GAAGA 3 cut(s) 272, 377, 566
MfeI CAATTG 1 cut(s) 697
MflI RGATCY 2 cut(s) 251, 617
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 3 cut(s) 499, 670, 697
MmeI TCCRAC 1 cut(s) 267
MroI TCCGGA 1 cut(s) 216
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 461
MspI CCGG 3 cut(s) 217, 521, 810
MspR9I CCNGG 3 cut(s) 257, 600, 716
MunI CAATTG 1 cut(s) 697
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 3 cut(s) 257, 600, 716
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 427, 533
NdeII GATC 4 cut(s) 251, 574, 617, 822
NlaIII CATG 3 cut(s) 28, 460, 779
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 2 cut(s) 452, 766
NspI RCATGY 1 cut(s) 28
PciI ACATGT 1 cut(s) 24
PcsI WCGNNNNNNNCGW 2 cut(s) 202, 634
PctI GAATGC 1 cut(s) 468
PfeI GAWTC 3 cut(s) 271, 382, 817
PkrI GCNGC 5 cut(s) 76, 110, 343, 432, 526
Ppu21I YACGTR 1 cut(s) 17
PscI ACATGT 1 cut(s) 24
Psp6I CCWGG 3 cut(s) 255, 598, 714
PspEI GGTNACC 1 cut(s) 766
PspFI CCCAGC 1 cut(s) 805
PspGI CCWGG 3 cut(s) 255, 598, 714
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 3 cut(s) 223, 238, 691
PstI CTGCAG 1 cut(s) 529
PsuI RGATCY 2 cut(s) 251, 617
RsaI GTAC 3 cut(s) 19, 246, 829
RsaNI GTAC 3 cut(s) 18, 245, 828
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 5 cut(s) 75, 109, 342, 431, 525
Sau3AI GATC 4 cut(s) 251, 574, 617, 822
Sau96I GGNCC 3 cut(s) 223, 238, 691
ScaI AGTACT 1 cut(s) 246
ScrFI CCNGG 3 cut(s) 257, 600, 716
SduI GDGCHC 1 cut(s) 68
SfaNI GCATC 4 cut(s) 189, 256, 523, 781
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 2 cut(s) 227, 475
SmoI CTYRAG 2 cut(s) 227, 475
Sse9I AATT 3 cut(s) 499, 670, 697
SsiI CCGC 5 cut(s) 106, 109, 431, 694, 710
SspMI CTAG 2 cut(s) 309, 605
StyD4I CCNGG 3 cut(s) 255, 598, 714
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 3 cut(s) 19, 553, 631
TaqI TCGA 4 cut(s) 174, 483, 503, 637
TasI AATT 3 cut(s) 499, 670, 697
TatI WGTACW 1 cut(s) 244
TauI GCSGC 2 cut(s) 111, 433
TfiI GAWTC 3 cut(s) 271, 382, 817
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 2 cut(s) 452, 766
TseI GCWGC 3 cut(s) 74, 341, 524
Tsp45I GTSAC 2 cut(s) 452, 766
TspDTI ATGAA 4 cut(s) 111, 127, 561, 765
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 1 cut(s) 534
VneI GTGCAC 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 238
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 28
XspI CTAG 2 cut(s) 309, 605
ZrmI AGTACT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.