FvH4_5g17150

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017202
Physical Location & Seq
Reverse (-)
9786606 .. 9789346
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17150.t1

Sequence Viewer

Length: 1746 bp
ATGAAGGCTTTCTGCCAGTTAGTACTATTGGTAACTCTATTGATCATATCTGGGGTTCTACCATCTTGTGTTTCGCAAACAACGCGGCGCTTTGAATTTAATGTTGAGTGGAAAAATGTGACCCGTTTGTGTCACAAAAAGCCACTTCTAACTGTTAATGGAGAGTATCCAGGTCCAACGATTGCTGTTCATGAAGGTGATAATGTCGAAATTAAGGTGACTAATCGCATTGCGCAGAACACTACCATTCATTGGCATGGTATAAGGCAAATTAGAACAGGATGGGCAGACGGTCCAGCTTACATTACGCAATGTCCAATCAGAGAAGGGAAGACCTACACATACAAGTTCACTGTAATTGATCAGAGAGGCACCCTTTGGTGGCATGCTCATCATTCGTGGCAACGAGCTTCAGTTTATGGAGCCTTTATCATCCACCCTCGCACGCCGTATCCATTTTCAGTTCCAATTCAAGATGAATTCCCCATCATCTTTGGTGAATGGTGGAATGGAGATATAGATTCAGTGGAAAGTGAAATGATGAAGTATGGAGGCGGCCCTGCCATTTCAAATGCCTATACCATAAACGGTTTACCTGGACCATTCTATAATTGCTCAAACAAAGATACATTGATCAAAACTGTGGAACATGGCAAAACATACATGCTTAGAATCATCAATGCAGCACTGAATGATGAGCTTTTCTTTGCTGTTGCTAATCACACATTGACGGTTGTGGAGATTGATGCAGTGTACACAAAACCATTCAAAACCAAAGCCATCATGATAGCTCCTGGCCAAACCACCAATGTTCTTCTCACTGCTAATCAAGTGCCTGATTCCTCAGGCATGTTCTTAATGGTTGCCTGGACTTACCTCACATCTGTTTTTCCTTTCAATAATTCCACCACAGCGGGTTTCTTGCAGTACAAGAACCTGATGAGTACTGAACACACGAGTCAGCACCCGAATAAGGTCACTACTTTTGATCCTGTCATGTACAACTTTCCTCAGATGGAAGACACCAAATATTACACTAAATTTTCTGACAACCTGAGAAGCCTTCACTCCCCTCGGTATCCATGTAATGTGCCGAAAACTATCCACAAACGGATAGTAGCAGTTATAAGCCTTAACCTTCAAGATTGTCCTGCTAACAAAACCTGCAAGGGCTACGCCAACAAGAGGTTCTTTGCTTCAATGAACAACCAGTCATTTGTTCGTCCCGCCTTGTCAATATTGGAATCTCATTACAAGAAGCTCAAAACAAGCCAATACTCCACCGATTTCCCAGAGAAGCCTCGAAAGCCATTTGACTATACCGGGGTGGATCCACTCACAGAAAACATGAACACAGAATTTGGTACCAAGATCATGCAAGTCCCCTATGGAACAAACTTGGAAATTGTGCTGCAAGGCACAAGCTTCCTTAATGTGGAGAACCACCCCATTCATGTACATGGACACAACTTCTTCATAGTTGGTAAGGGGTTTGGGAACTTTGATGTCTCCAAGGATCCAGCAAAGTACAACCTTGTTGATCCTCCTGAGAGAAACACAGTAGCAGTACCAAGTGGAGGATGGGCCGCGATTCGGATCAAGGCTGATAATCCAGGAGTGTGGTTCATACATTGTCATCTTGAAGAACACACTTCCTGGGGTCTTGCCTCAGGCTTCATAGTTCAAAATGGTCATGGACCATCTGAATCCTTGCTTCCTCCTCCCAAGGATCTTCCCTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

582

Amino Acids

65.55

Weight (kDa)

8.6

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 34 - 148 1.1e-44 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 3.3e-40 Multicopper oxidase
Cu-oxidase_2 PF07731 430 - 564 7.9e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18140
fragaria_vesca FvH4_5g17150
malus_domestica MD06G1230100.v1.1 MD14G1234800.v1.1
prunus_persica Prupe.5G231100_v2.0.a1
pyrus_communis pycom14g19580
rosa_chinensis RchiOBHm_Chr7g0178641
rosa_laevigata RLG00000005427
rosa_multiflora Rmu_sc0002320.1_g000007
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017400 Rh7BG017000
rosa_wichuraiana Rw7G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1127
Acc16I TGCGCA 1 cut(s) 234
Acc36I ACCTGC 1 cut(s) 1172
Acc65I GGTACC 1 cut(s) 1364
AccB1I GGYRCC 2 cut(s) 371, 1364
AccB7I CCANNNNNTGG 1 cut(s) 252
AccII CGCG 2 cut(s) 85, 1589
AciI CCGC 5 cut(s) 85, 555, 914, 1227, 1587
AclWI GGATC 8 cut(s) 983, 1325, 1338, 1511, 1524, 1535, 1604, 1737
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 4 cut(s) 95, 479, 1040, 1358
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 9 cut(s) 24, 755, 929, 946, 1001, 1366, 1458, 1529, 1569
AfiI CCNNNNNNNGG 7 cut(s) 252, 381, 973, 1185, 1435, 1577, 1593
AgsI TTSAA 9 cut(s) 95, 473, 570, 769, 898, 1142, 1200, 1643, 1685
AjnI CCWGG 6 cut(s) 169, 595, 793, 866, 1612, 1655
AjuI GAANNNNNNNTTGG 2 cut(s) 1172, 1204
AluBI AGCT 6 cut(s) 299, 410, 700, 791, 1261, 1425
AluI AGCT 6 cut(s) 299, 410, 700, 791, 1261, 1425
Alw26I GTCTC 1 cut(s) 1513
AlwI GGATC 8 cut(s) 983, 1325, 1338, 1511, 1524, 1535, 1604, 1737
AoxI GGCC 3 cut(s) 556, 796, 1584
ApeKI GCWGC 2 cut(s) 683, 1411
ApoI RAATTY 4 cut(s) 95, 479, 1040, 1358
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 1364
AspLEI GCGC 2 cut(s) 90, 235
AspS9I GGNCC 6 cut(s) 173, 293, 557, 599, 1584, 1697
AsuC2I CCSGG 1 cut(s) 1324
AsuHPI GGTGA 3 cut(s) 209, 229, 509
AvaII GGWCC 4 cut(s) 173, 293, 599, 1697
AxyI CCTNAGG 2 cut(s) 844, 1669
BalI TGGCCA 1 cut(s) 798
BamHI GGATCC 2 cut(s) 1330, 1516
BanI GGYRCC 2 cut(s) 371, 1364
BauI CACGAG 1 cut(s) 955
BbsI GAAGAC 2 cut(s) 338, 1026
BbvI GCAGC 2 cut(s) 695, 1398
BccI CCATC 7 cut(s) 70, 276, 494, 788, 1009, 1575, 1708
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 6 cut(s) 171, 597, 795, 868, 1614, 1657
BciVI GTATCC 3 cut(s) 177, 462, 1089
BclI TGATCA 3 cut(s) 42, 361, 633
BcnI CCSGG 1 cut(s) 1324
BcoDI GTCTC 1 cut(s) 1513
BfoI RGCGCY 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 1172
BfuI GTATCC 3 cut(s) 177, 462, 1089
BisI GCNGC 5 cut(s) 86, 556, 684, 1412, 1587
BlsI GCNGC 5 cut(s) 87, 557, 685, 1413, 1588
BmcAI AGTACT 2 cut(s) 24, 946
Bme1390I CCNGG 7 cut(s) 171, 597, 795, 868, 1324, 1614, 1657
Bme18I GGWCC 4 cut(s) 173, 293, 599, 1697
BmgT120I GGNCC 6 cut(s) 173, 293, 557, 599, 1584, 1697
BmiI GGNNCC 5 cut(s) 373, 424, 1332, 1366, 1518
BmrFI CCNGG 7 cut(s) 171, 597, 795, 868, 1324, 1614, 1657
BmsI GCATC 1 cut(s) 736
BpiI GAAGAC 2 cut(s) 338, 1026
BpuMI CCSGG 1 cut(s) 1324
BsaBI GATNNNNATC 1 cut(s) 1595
BsaJI CCNNGG 5 cut(s) 1073, 1323, 1512, 1656, 1725
Bsc4I CCNNNNNNNGG 7 cut(s) 252, 381, 973, 1185, 1435, 1577, 1593
Bse1I ACTGG 2 cut(s) 16, 1210
Bse21I CCTNAGG 2 cut(s) 844, 1669
Bse3DI GCAATG 2 cut(s) 228, 317
Bse8I GATNNNNATC 1 cut(s) 1595
BseBI CCWGG 6 cut(s) 171, 597, 795, 868, 1614, 1657
BseDI CCNNGG 5 cut(s) 1073, 1323, 1512, 1656, 1725
BseGI GGATG 3 cut(s) 287, 432, 1586
BseJI GATNNNNATC 1 cut(s) 1595
BseLI CCNNNNNNNGG 7 cut(s) 252, 381, 973, 1185, 1435, 1577, 1593
BseMI GCAATG 2 cut(s) 228, 317
BseMII CTCAG 5 cut(s) 858, 1025, 1046, 1539, 1683
BseNI ACTGG 2 cut(s) 16, 1210
BseRI GAGGAG 1 cut(s) 1710
BseXI GCAGC 2 cut(s) 695, 1398
Bsh1236I CGCG 2 cut(s) 85, 1589
BshFI GGCC 3 cut(s) 558, 798, 1586
BshNI GGYRCC 2 cut(s) 371, 1364
BsiSI CCGG 1 cut(s) 1323
BslFI GGGAC 2 cut(s) 1209, 1367
BslI CCNNNNNNNGG 7 cut(s) 252, 381, 973, 1185, 1435, 1577, 1593
BsmAI GTCTC 1 cut(s) 1513
BsmFI GGGAC 2 cut(s) 1209, 1367
BsnI GGCC 3 cut(s) 558, 798, 1586
Bsp1407I TGTACA 3 cut(s) 753, 999, 1456
BspACI CCGC 5 cut(s) 85, 555, 914, 1227, 1587
BspANI GGCC 3 cut(s) 558, 798, 1586
BspCNI CTCAG 5 cut(s) 857, 1024, 1047, 1540, 1682
BspFNI CGCG 2 cut(s) 85, 1589
BspHI TCATGA 2 cut(s) 190, 783
BspLI GGNNCC 5 cut(s) 373, 424, 1332, 1366, 1518
BspMI ACCTGC 1 cut(s) 1172
BspPI GGATC 8 cut(s) 983, 1325, 1338, 1511, 1524, 1535, 1604, 1737
BspT107I GGYRCC 2 cut(s) 371, 1364
BsrDI GCAATG 2 cut(s) 228, 317
BsrGI TGTACA 3 cut(s) 753, 999, 1456
BsrI ACTGG 2 cut(s) 16, 1210
BssECI CCNNGG 5 cut(s) 1073, 1323, 1512, 1656, 1725
BssSI CACGAG 1 cut(s) 955
BssT1I CCWWGG 2 cut(s) 1512, 1725
Bst2BI CACGAG 1 cut(s) 955
Bst2UI CCWGG 6 cut(s) 171, 597, 795, 868, 1614, 1657
Bst4CI ACNGT 7 cut(s) 154, 293, 355, 590, 643, 733, 1561
BstAUI TGTACA 3 cut(s) 753, 999, 1456
BstC8I GCNNGC 2 cut(s) 387, 446
BstDEI CTNAG 6 cut(s) 668, 844, 1011, 1055, 1548, 1669
BstF5I GGATG 3 cut(s) 287, 432, 1586
BstFNI CGCG 2 cut(s) 85, 1589
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 2 cut(s) 90, 235
BstMAI GTCTC 1 cut(s) 1513
BstMWI GCNNNNNNNGC 2 cut(s) 82, 1306
BstNI CCWGG 6 cut(s) 171, 597, 795, 868, 1614, 1657
BstNSI RCATGY 3 cut(s) 389, 667, 853
BstSCI CCNGG 7 cut(s) 169, 595, 793, 866, 1322, 1612, 1655
BstUI CGCG 2 cut(s) 85, 1589
BstV1I GCAGC 2 cut(s) 695, 1398
BstV2I GAAGAC 2 cut(s) 338, 1026
BstX2I RGATCY 3 cut(s) 1330, 1516, 1729
BstXI CCANNNNNNTGG 1 cut(s) 1620
BstYI RGATCY 3 cut(s) 1330, 1516, 1729
Bsu36I CCTNAGG 2 cut(s) 844, 1669
BsuI GTATCC 3 cut(s) 177, 462, 1089
BsuRI GGCC 3 cut(s) 558, 798, 1586
BtsCI GGATG 3 cut(s) 287, 432, 1586
BtsI GCAGTG 2 cut(s) 756, 819
BtsIMutI CAGTG 5 cut(s) 351, 531, 686, 756, 819
BveI ACCTGC 1 cut(s) 1172
Cac8I GCNNGC 2 cut(s) 387, 446
CciI TCATGA 2 cut(s) 190, 783
CfoI GCGC 2 cut(s) 90, 235
Cfr13I GGNCC 6 cut(s) 173, 293, 557, 599, 1584, 1697
Csp6I GTAC 9 cut(s) 23, 754, 928, 945, 1000, 1365, 1457, 1528, 1568
CviQI GTAC 9 cut(s) 23, 754, 928, 945, 1000, 1365, 1457, 1528, 1568
DdeI CTNAG 6 cut(s) 668, 844, 1011, 1055, 1548, 1669
EaeI YGGCCR 1 cut(s) 796
Eco130I CCWWGG 2 cut(s) 1512, 1725
Eco47I GGWCC 4 cut(s) 173, 293, 599, 1697
Eco57I CTGAAG 1 cut(s) 396
Eco81I CCTNAGG 2 cut(s) 844, 1669
EcoRI GAATTC 1 cut(s) 479
EcoRII CCWGG 6 cut(s) 169, 595, 793, 866, 1612, 1655
EcoT14I CCWWGG 2 cut(s) 1512, 1725
ErhI CCWWGG 2 cut(s) 1512, 1725
FalI AAGNNNNNCTT 2 cut(s) 1175, 1207
FaqI GGGAC 2 cut(s) 1209, 1367
FauI CCCGC 2 cut(s) 907, 1234
FbaI TGATCA 3 cut(s) 42, 361, 633
Fnu4HI GCNGC 5 cut(s) 86, 556, 684, 1412, 1587
FokI GGATG 3 cut(s) 294, 419, 1593
Fsp4HI GCNGC 5 cut(s) 86, 556, 684, 1412, 1587
FspI TGCGCA 1 cut(s) 234
GlaI GCGC 2 cut(s) 89, 234
GluI GCNGC 5 cut(s) 86, 556, 684, 1412, 1587
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 3 cut(s) 558, 798, 1586
HapII CCGG 1 cut(s) 1323
HhaI GCGC 2 cut(s) 90, 235
Hin6I GCGC 2 cut(s) 88, 233
HinP1I GCGC 2 cut(s) 88, 233
HindIII AAGCTT 1 cut(s) 1423
HinfI GANTC 7 cut(s) 521, 672, 839, 958, 1244, 1591, 1706
HpaII CCGG 1 cut(s) 1323
HphI GGTGA 3 cut(s) 209, 229, 509
Hpy166II GTNNAC 4 cut(s) 351, 593, 754, 756
Hpy188I TCNGA 6 cut(s) 323, 366, 1014, 1048, 1596, 1705
Hpy188III TCNNGA 6 cut(s) 191, 473, 784, 1142, 1547, 1640
Hpy8I GTNNAC 4 cut(s) 351, 593, 754, 756
HpyAV CCTTC 4 cut(s) 188, 320, 1073, 1148
HpyCH4III ACNGT 7 cut(s) 154, 293, 355, 590, 643, 733, 1561
HpyCH4V TGCA 6 cut(s) 683, 749, 925, 1167, 1378, 1414
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 1306
HpyF3I CTNAG 6 cut(s) 668, 844, 1011, 1055, 1548, 1669
HspAI GCGC 2 cut(s) 88, 233
KpnI GGTACC 1 cut(s) 1368
Ksp22I TGATCA 3 cut(s) 42, 361, 633
LmnI GCTCC 2 cut(s) 422, 796
Lsp1109I GCAGC 2 cut(s) 695, 1398
LweI GCATC 1 cut(s) 736
MaeIII GTNAC 5 cut(s) 31, 118, 131, 217, 976
MboII GAAGA 6 cut(s) 343, 806, 1031, 1465, 1655, 1724
MflI RGATCY 3 cut(s) 1330, 1516, 1729
MlsI TGGCCA 1 cut(s) 798
MluNI TGGCCA 1 cut(s) 798
MlyI GAGTC 1 cut(s) 967
MmeI TCCRAC 1 cut(s) 200
Mox20I TGGCCA 1 cut(s) 798
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 798
MseI TTAA 6 cut(s) 99, 156, 213, 857, 1134, 1431
MslI CAYNNNNRTG 3 cut(s) 195, 255, 1458
Msp20I TGGCCA 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 1 cut(s) 1323
MspR9I CCNGG 7 cut(s) 171, 597, 795, 868, 1324, 1614, 1657
MvaI CCWGG 6 cut(s) 171, 597, 795, 868, 1614, 1657
MvnI CGCG 2 cut(s) 85, 1589
MwoI GCNNNNNNNGC 2 cut(s) 82, 1306
NciI CCSGG 1 cut(s) 1324
NlaIV GGNNCC 5 cut(s) 373, 424, 1332, 1366, 1518
NmuCI GTSAC 4 cut(s) 118, 131, 217, 976
NsbI TGCGCA 1 cut(s) 234
NspI RCATGY 3 cut(s) 389, 667, 853
PaeI GCATGC 1 cut(s) 389
PagI TCATGA 2 cut(s) 190, 783
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 6 cut(s) 521, 672, 839, 1244, 1591, 1706
PflMI CCANNNNNTGG 1 cut(s) 252
PfoI TCCNGGA 1 cut(s) 1612
PkrI GCNGC 5 cut(s) 87, 557, 685, 1413, 1588
PleI GAGTC 1 cut(s) 966
PpsI GAGTC 1 cut(s) 966
PsiI TTATAA 1 cut(s) 1127
Psp6I CCWGG 6 cut(s) 169, 595, 793, 866, 1612, 1655
PspGI CCWGG 6 cut(s) 169, 595, 793, 866, 1612, 1655
PspN4I GGNNCC 5 cut(s) 373, 424, 1332, 1366, 1518
PspPI GGNCC 6 cut(s) 173, 293, 557, 599, 1584, 1697
PsuI RGATCY 3 cut(s) 1330, 1516, 1729
RsaI GTAC 9 cut(s) 24, 755, 929, 946, 1001, 1366, 1458, 1529, 1569
RsaNI GTAC 9 cut(s) 23, 754, 928, 945, 1000, 1365, 1457, 1528, 1568
RseI CAYNNNNRTG 3 cut(s) 195, 255, 1458
SaqAI TTAA 6 cut(s) 99, 156, 213, 857, 1134, 1431
SatI GCNGC 5 cut(s) 86, 556, 684, 1412, 1587
Sau96I GGNCC 6 cut(s) 173, 293, 557, 599, 1584, 1697
ScaI AGTACT 2 cut(s) 24, 946
SchI GAGTC 1 cut(s) 967
ScrFI CCNGG 7 cut(s) 171, 597, 795, 868, 1324, 1614, 1657
SfaNI GCATC 1 cut(s) 736
SinI GGWCC 4 cut(s) 173, 293, 599, 1697
SmiMI CAYNNNNRTG 3 cut(s) 195, 255, 1458
SphI GCATGC 1 cut(s) 389
SsiI CCGC 5 cut(s) 85, 555, 914, 1227, 1587
SspI AATATT 2 cut(s) 1031, 1239
StyD4I CCNGG 7 cut(s) 169, 595, 793, 866, 1322, 1612, 1655
StyI CCWWGG 2 cut(s) 1512, 1725
TaaI ACNGT 7 cut(s) 154, 293, 355, 590, 643, 733, 1561
TaqI TCGA 2 cut(s) 207, 1303
TatI WGTACW 7 cut(s) 22, 753, 927, 944, 999, 1456, 1527
TauI GCSGC 3 cut(s) 88, 558, 1589
TfiI GAWTC 6 cut(s) 521, 672, 839, 1244, 1591, 1706
Tru1I TTAA 6 cut(s) 99, 156, 213, 857, 1134, 1431
Tru9I TTAA 6 cut(s) 99, 156, 213, 857, 1134, 1431
TscAI CASTG 5 cut(s) 358, 531, 693, 756, 826
TseFI GTSAC 4 cut(s) 118, 131, 217, 976
TseI GCWGC 2 cut(s) 683, 1411
Tsp45I GTSAC 4 cut(s) 118, 131, 217, 976
TspGWI ACGGA 1 cut(s) 1126
TspRI CASTG 5 cut(s) 358, 531, 693, 756, 826
Van91I CCANNNNNTGG 1 cut(s) 252
VpaK11BI GGWCC 4 cut(s) 173, 293, 599, 1697
XapI RAATTY 4 cut(s) 95, 479, 1040, 1358
XceI RCATGY 3 cut(s) 389, 667, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 1578
XmnI GAANNNNTTC 1 cut(s) 8
ZrmI AGTACT 2 cut(s) 24, 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.