RLG00000005427

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017202
Physical Location & Seq
Reverse (-)
67575112 .. 67577403
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005427

Sequence Viewer

Length: 1743 bp
ATGGAGGGTTTTTGCCGGTTAGTATTGGTGACTCTGTTGATCATAGCTGGGCTTCTACCATCTTGTGTTTCGCAAACAACCCAGCGCTTTGAATTTAATGTCGAGTGGAAAAATGTGACCCGTTTGTGCCACACGAAGCCACTTCTAACTGTGAATGGAGAGTATCCAGGTCCAACGATTGCAGTTCATGAAGGTGATAATGTAGAAATTAAGGTGACTAACCGCATTGAGCAGAACACTACCCTCCATTGGCATGGTATAAGGCAACTAAGAACAGGATGGGCAGACGGTCCAGCTTACATTACTCAATGTCCGATTGGAGGAGGGAAGACCTACACATACAAGTTCACTGTAATCAACCAGAGAGGCACCCTTTGGTGGCATGCTCATCATTCGTGGCAACGAGCTTCTGTTTATGGCGCTTTTATCATCCACCCTCGCATGACATATCCATTTTCAGTTCCAATTCAAGATGAATTCCCCATCATCTTTGGTGAATGGTGGAATGGAGATATAGATTCAGTCGAAAGTGAAATGATGAAGTATGGAGGCGGCCCTGATATTTCAAATGCATATACCATAAACGGTTTGCCAGGACCATTCTACAATTGCTCGACCAAAGATACATTGATCAACACTGTGGAACATGGAAAAACATACATGCTAAGAATCATCAATGCAGCACTGAATGATGAGCTATTCTTTGCTGTTGCTAATCACACACTGACGGTTGTGGAGATTGATGCAGCGTACACAAAACCATTCAAAACCAAAGCCATTATGATAGCTCCTGGCCAAACCACCAATGTTCTGCTCACTGCTAATCAAGTGCCTGATTCCTCCGGCATGTTCTTAATGGCTGCCTGGACTTACCTCACATCTGTTTTCCCTTTCAATAATTCCACCACAGCCGGTTTCTTGCAGTACAAGAACACAATGAGTACTGATCACATAAGTAAGCACCCGAAAAAGGTTACTGCTTTTGATCCTGTCATGTACAACTTTCCTCAGATGGATGATACCAAATATTACACTAAATTTGATGACAATCTGAGAAGCCTTTCCTCCCCTCGGTATCCATGTAATGTGCCAAAAACTATCCACAAACGGATGGTGACAGTTATAAGCCTTAACCTCCAAGATTGTCCTGCTAACAAAACCTGCAAGGGCTATGCCAACAAGAGGTTCTATGCTTCAATGAACAACCAGTCCTTTGTTCGTCCCGCCGTGTCAATATTGGAATCTCATTACAAGAAGCTAAAAAAAAGTCAATACTCCACCGATTTCCCAGAGAAGCCTCGAAGGCCATTTGACTATACCGGGGTGAATCCAGTCGCCGAAAACATGAACACAGAATTTGGTACAAAGATCATACAAGTCCCTTATGGAACAAACTTGGAATTTGTGCTGCAAGGCACAAGCTTCCTTAATGTGGAGAACCACCCTATTCATTTTCATGGACACAACTTCTTCATAGTTGGAAAGGGGTTTGGGAACTTCAATGTCTCCACGGATCCAGCAAAGTACAACCTTGTTGATCCTCCTGAGAGAAACACTGTGGCAGTTCCAAGTGGAGGATGGGCAGCGATTCGGTTCAAGGCCGATAATCCAGGAGTGTGGTTCATACATTGTCATCTTGAAGAACACACTTCTTGGGGTCTTGCCTCAGGCTTCATAGTTCAAAATGGTCATCGACCATCTCAATCCTTGCTTCCTCCTCCTAAAGATCTTCCCTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

581

Amino Acids

65.44

Weight (kDa)

8.61

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 147 9.1e-44 Multicopper oxidase
Cu-oxidase PF00394 159 - 311 1.1e-39 Multicopper oxidase
Cu-oxidase_2 PF07731 429 - 563 4.7e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18140
fragaria_vesca FvH4_5g17150
malus_domestica MD06G1230100.v1.1 MD14G1234800.v1.1
prunus_persica Prupe.5G231100_v2.0.a1
pyrus_communis pycom14g19580
rosa_chinensis RchiOBHm_Chr7g0178641
rosa_laevigata RLG00000005427
rosa_multiflora Rmu_sc0002320.1_g000007
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017400 Rh7BG017000
rosa_wichuraiana Rw7G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1169
AccB1I GGYRCC 1 cut(s) 368
AciI CCGC 3 cut(s) 223, 552, 1224
AclWI GGATC 4 cut(s) 980, 1508, 1521, 1532
AcoI YGGCCR 1 cut(s) 793
AcsI RAATTY 5 cut(s) 92, 476, 1037, 1355, 1400
AfaI GTAC 6 cut(s) 752, 926, 943, 998, 1363, 1526
AfeI AGCGCT 1 cut(s) 86
AfiI CCNNNNNNNGG 8 cut(s) 249, 320, 378, 970, 1071, 1182, 1432, 1574
AjnI CCWGG 5 cut(s) 166, 592, 790, 863, 1609
AjuI GAANNNNNNNTTGG 2 cut(s) 1169, 1201
AloI GAACNNNNNNTCC 2 cut(s) 1193, 1225
AluBI AGCT 7 cut(s) 47, 296, 407, 697, 788, 1258, 1422
AluI AGCT 7 cut(s) 47, 296, 407, 697, 788, 1258, 1422
Alw26I GTCTC 1 cut(s) 1510
AlwI GGATC 4 cut(s) 980, 1508, 1521, 1532
Aor51HI AGCGCT 1 cut(s) 86
AoxI GGCC 4 cut(s) 553, 793, 1304, 1599
ApeKI GCWGC 5 cut(s) 680, 746, 860, 1408, 1583
ApoI RAATTY 5 cut(s) 92, 476, 1037, 1355, 1400
Asp700I GAANNNNTTC 1 cut(s) 1060
AspLEI GCGC 2 cut(s) 87, 422
AspS9I GGNCC 4 cut(s) 170, 290, 554, 596
AsuC2I CCSGG 1 cut(s) 1321
AsuHPI GGTGA 6 cut(s) 40, 206, 226, 506, 1126, 1336
AvaII GGWCC 3 cut(s) 170, 290, 596
AxyI CCTNAGG 1 cut(s) 1666
BalI TGGCCA 1 cut(s) 795
BamHI GGATCC 1 cut(s) 1513
BanI GGYRCC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 335
BbvI GCAGC 5 cut(s) 692, 758, 847, 1395, 1595
BccI CCATC 7 cut(s) 67, 273, 491, 1006, 1105, 1572, 1705
BceAI ACGGC 1 cut(s) 1211
BciT130I CCWGG 5 cut(s) 168, 594, 792, 865, 1611
BciVI GTATCC 2 cut(s) 174, 1086
BclI TGATCA 3 cut(s) 39, 630, 946
BcnI CCSGG 1 cut(s) 1321
BcoDI GTCTC 1 cut(s) 1510
BfoI RGCGCY 2 cut(s) 88, 423
BfuAI ACCTGC 1 cut(s) 1169
BfuI GTATCC 2 cut(s) 174, 1086
BglI GCCNNNNNGGC 1 cut(s) 1303
BglII AGATCT 1 cut(s) 1726
BisI GCNGC 6 cut(s) 553, 681, 747, 861, 1409, 1584
BlsI GCNGC 6 cut(s) 554, 682, 748, 862, 1410, 1585
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 6 cut(s) 168, 594, 792, 865, 1321, 1611
Bme18I GGWCC 3 cut(s) 170, 290, 596
BmgT120I GGNCC 4 cut(s) 170, 290, 554, 596
BmiI GGNNCC 2 cut(s) 370, 1515
BmrFI CCNGG 6 cut(s) 168, 594, 792, 865, 1321, 1611
BmsI GCATC 1 cut(s) 733
BpiI GAAGAC 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 1321
BsaBI GATNNNNATC 1 cut(s) 1047
BsaJI CCNNGG 3 cut(s) 1070, 1320, 1509
Bsc4I CCNNNNNNNGG 8 cut(s) 249, 320, 378, 970, 1071, 1182, 1432, 1574
Bse118I RCCGGY 2 cut(s) 15, 911
Bse1I ACTGG 2 cut(s) 1207, 1331
Bse21I CCTNAGG 1 cut(s) 1666
Bse8I GATNNNNATC 1 cut(s) 1047
BseBI CCWGG 5 cut(s) 168, 594, 792, 865, 1611
BseDI CCNNGG 3 cut(s) 1070, 1320, 1509
BseGI GGATG 5 cut(s) 284, 429, 1021, 1116, 1583
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 8 cut(s) 249, 320, 378, 970, 1071, 1182, 1432, 1574
BseMII CTCAG 4 cut(s) 1022, 1043, 1536, 1680
BseNI ACTGG 2 cut(s) 1207, 1331
BseRI GAGGAG 2 cut(s) 336, 1707
BseXI GCAGC 5 cut(s) 692, 758, 847, 1395, 1595
BseYI CCCAGC 2 cut(s) 47, 81
BshFI GGCC 4 cut(s) 555, 795, 1306, 1601
BshNI GGYRCC 1 cut(s) 368
BsiSI CCGG 4 cut(s) 16, 843, 912, 1320
BslFI GGGAC 2 cut(s) 1206, 1364
BslI CCNNNNNNNGG 8 cut(s) 249, 320, 378, 970, 1071, 1182, 1432, 1574
BsmAI GTCTC 1 cut(s) 1510
BsmFI GGGAC 2 cut(s) 1206, 1364
BsnI GGCC 4 cut(s) 555, 795, 1306, 1601
Bsp1407I TGTACA 1 cut(s) 996
Bsp143I GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
BspACI CCGC 3 cut(s) 223, 552, 1224
BspANI GGCC 4 cut(s) 555, 795, 1306, 1601
BspCNI CTCAG 4 cut(s) 1021, 1044, 1537, 1679
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 370, 1515
BspMI ACCTGC 1 cut(s) 1169
BspPI GGATC 4 cut(s) 980, 1508, 1521, 1532
BspT107I GGYRCC 1 cut(s) 368
BsrFI RCCGGY 2 cut(s) 15, 911
BsrGI TGTACA 1 cut(s) 996
BsrI ACTGG 2 cut(s) 1207, 1331
BssAI RCCGGY 2 cut(s) 15, 911
BssECI CCNNGG 3 cut(s) 1070, 1320, 1509
BssMI GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
Bst2UI CCWGG 5 cut(s) 168, 594, 792, 865, 1611
Bst4CI ACNGT 8 cut(s) 151, 290, 352, 587, 640, 730, 1120, 1558
BstAUI TGTACA 1 cut(s) 996
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 6 cut(s) 269, 665, 1008, 1052, 1545, 1666
BstDSI CCRYGG 1 cut(s) 1509
BstF5I GGATG 5 cut(s) 284, 429, 1021, 1116, 1583
BstH2I RGCGCY 2 cut(s) 88, 423
BstHHI GCGC 2 cut(s) 87, 422
BstKTI GATC 8 cut(s) 42, 633, 949, 988, 1371, 1516, 1540, 1729
BstMAI GTCTC 1 cut(s) 1510
BstMBI GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
BstMWI GCNNNNNNNGC 1 cut(s) 1303
BstNI CCWGG 5 cut(s) 168, 594, 792, 865, 1611
BstNSI RCATGY 3 cut(s) 386, 664, 850
BstSCI CCNGG 6 cut(s) 166, 592, 790, 863, 1319, 1609
BstV1I GCAGC 5 cut(s) 692, 758, 847, 1395, 1595
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 1513, 1726
BstXI CCANNNNNNTGG 2 cut(s) 254, 1617
BstYI RGATCY 2 cut(s) 1513, 1726
Bsu36I CCTNAGG 1 cut(s) 1666
BsuI GTATCC 2 cut(s) 174, 1086
BsuRI GGCC 4 cut(s) 555, 795, 1306, 1601
BtgI CCRYGG 1 cut(s) 1509
BtsCI GGATG 5 cut(s) 284, 429, 1021, 1116, 1583
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 6 cut(s) 348, 636, 683, 722, 816, 1554
BveI ACCTGC 1 cut(s) 1169
Cac8I GCNNGC 1 cut(s) 384
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 87, 422
Cfr10I RCCGGY 2 cut(s) 15, 911
Cfr13I GGNCC 4 cut(s) 170, 290, 554, 596
Csp6I GTAC 6 cut(s) 751, 925, 942, 997, 1362, 1525
CviQI GTAC 6 cut(s) 751, 925, 942, 997, 1362, 1525
DdeI CTNAG 6 cut(s) 269, 665, 1008, 1052, 1545, 1666
DpnI GATC 8 cut(s) 41, 632, 948, 987, 1370, 1515, 1539, 1728
DpnII GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
EaeI YGGCCR 1 cut(s) 793
Eco47I GGWCC 3 cut(s) 170, 290, 596
Eco47III AGCGCT 1 cut(s) 86
Eco81I CCTNAGG 1 cut(s) 1666
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 5 cut(s) 166, 592, 790, 863, 1609
EcoT22I ATGCAT 1 cut(s) 574
FaqI GGGAC 2 cut(s) 1206, 1364
FauI CCCGC 1 cut(s) 1231
FbaI TGATCA 3 cut(s) 39, 630, 946
Fnu4HI GCNGC 6 cut(s) 553, 681, 747, 861, 1409, 1584
FokI GGATG 5 cut(s) 291, 416, 1028, 1123, 1590
Fsp4HI GCNGC 6 cut(s) 553, 681, 747, 861, 1409, 1584
GlaI GCGC 2 cut(s) 86, 421
GluI GCNGC 6 cut(s) 553, 681, 747, 861, 1409, 1584
GsaI CCCAGC 2 cut(s) 51, 85
HaeII RGCGCY 2 cut(s) 88, 423
HaeIII GGCC 4 cut(s) 555, 795, 1306, 1601
HapII CCGG 4 cut(s) 16, 843, 912, 1320
HhaI GCGC 2 cut(s) 87, 422
Hin6I GCGC 2 cut(s) 85, 420
HinP1I GCGC 2 cut(s) 85, 420
HindIII AAGCTT 1 cut(s) 1420
HinfI GANTC 7 cut(s) 31, 518, 669, 836, 1241, 1327, 1588
HpaII CCGG 4 cut(s) 16, 843, 912, 1320
HphI GGTGA 6 cut(s) 40, 206, 226, 506, 1126, 1336
Hpy166II GTNNAC 2 cut(s) 348, 753
Hpy188I TCNGA 3 cut(s) 315, 1011, 1053
Hpy188III TCNNGA 4 cut(s) 188, 470, 1544, 1637
Hpy8I GTNNAC 2 cut(s) 348, 753
HpyAV CCTTC 2 cut(s) 185, 1296
HpyCH4III ACNGT 8 cut(s) 151, 290, 352, 587, 640, 730, 1120, 1558
HpyCH4V TGCA 7 cut(s) 182, 572, 680, 746, 922, 1164, 1411
HpyF10VI GCNNNNNNNGC 1 cut(s) 1303
HpyF3I CTNAG 6 cut(s) 269, 665, 1008, 1052, 1545, 1666
HspAI GCGC 2 cut(s) 85, 420
Ksp22I TGATCA 3 cut(s) 39, 630, 946
Kzo9I GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
LmnI GCTCC 1 cut(s) 793
Lsp1109I GCAGC 5 cut(s) 692, 758, 847, 1395, 1595
LweI GCATC 1 cut(s) 733
MaeIII GTNAC 5 cut(s) 28, 115, 214, 973, 1114
MalI GATC 8 cut(s) 41, 632, 948, 987, 1370, 1515, 1539, 1728
MboI GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
MboII GAAGA 4 cut(s) 340, 1462, 1652, 1721
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 2 cut(s) 1513, 1726
MlsI TGGCCA 1 cut(s) 795
MluCI AATT 9 cut(s) 92, 207, 465, 476, 607, 898, 1037, 1355, 1400
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 2 cut(s) 197, 1459
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 1060
MscI TGGCCA 1 cut(s) 795
MseI TTAA 5 cut(s) 96, 210, 854, 1131, 1428
MslI CAYNNNNRTG 3 cut(s) 192, 252, 1455
Msp20I TGGCCA 1 cut(s) 795
MspI CCGG 4 cut(s) 16, 843, 912, 1320
MspR9I CCNGG 6 cut(s) 168, 594, 792, 865, 1321, 1611
MunI CAATTG 1 cut(s) 607
MvaI CCWGG 5 cut(s) 168, 594, 792, 865, 1611
MwoI GCNNNNNNNGC 1 cut(s) 1303
NciI CCSGG 1 cut(s) 1321
NdeII GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
NlaIV GGNNCC 2 cut(s) 370, 1515
NmuCI GTSAC 4 cut(s) 28, 115, 214, 1114
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 3 cut(s) 386, 664, 850
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 1060
PfeI GAWTC 6 cut(s) 518, 669, 836, 1241, 1327, 1588
PfoI TCCNGGA 1 cut(s) 1609
PkrI GCNGC 6 cut(s) 554, 682, 748, 862, 1410, 1585
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PsiI TTATAA 1 cut(s) 1124
Psp6I CCWGG 5 cut(s) 166, 592, 790, 863, 1609
PspFI CCCAGC 2 cut(s) 47, 81
PspGI CCWGG 5 cut(s) 166, 592, 790, 863, 1609
PspN4I GGNNCC 2 cut(s) 370, 1515
PspPI GGNCC 4 cut(s) 170, 290, 554, 596
PsuI RGATCY 2 cut(s) 1513, 1726
RsaI GTAC 6 cut(s) 752, 926, 943, 998, 1363, 1526
RsaNI GTAC 6 cut(s) 751, 925, 942, 997, 1362, 1525
RseI CAYNNNNRTG 3 cut(s) 192, 252, 1455
SaqAI TTAA 5 cut(s) 96, 210, 854, 1131, 1428
SatI GCNGC 6 cut(s) 553, 681, 747, 861, 1409, 1584
Sau3AI GATC 8 cut(s) 39, 630, 946, 985, 1368, 1513, 1537, 1726
Sau96I GGNCC 4 cut(s) 170, 290, 554, 596
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 6 cut(s) 168, 594, 792, 865, 1321, 1611
SfaNI GCATC 1 cut(s) 733
SinI GGWCC 3 cut(s) 170, 290, 596
SmiMI CAYNNNNRTG 3 cut(s) 192, 252, 1455
SphI GCATGC 1 cut(s) 386
Sse9I AATT 9 cut(s) 92, 207, 465, 476, 607, 898, 1037, 1355, 1400
SsiI CCGC 3 cut(s) 223, 552, 1224
SspI AATATT 2 cut(s) 1028, 1236
StyD4I CCNGG 6 cut(s) 166, 592, 790, 863, 1319, 1609
TaaI ACNGT 8 cut(s) 151, 290, 352, 587, 640, 730, 1120, 1558
TaqI TCGA 5 cut(s) 102, 525, 614, 1300, 1693
TasI AATT 9 cut(s) 92, 207, 465, 476, 607, 898, 1037, 1355, 1400
TatI WGTACW 4 cut(s) 924, 941, 996, 1524
TauI GCSGC 1 cut(s) 555
TfiI GAWTC 6 cut(s) 518, 669, 836, 1241, 1327, 1588
Tru1I TTAA 5 cut(s) 96, 210, 854, 1131, 1428
Tru9I TTAA 5 cut(s) 96, 210, 854, 1131, 1428
TscAI CASTG 6 cut(s) 355, 643, 690, 729, 823, 1561
TseFI GTSAC 4 cut(s) 28, 115, 214, 1114
TseI GCWGC 5 cut(s) 680, 746, 860, 1408, 1583
Tsp45I GTSAC 4 cut(s) 28, 115, 214, 1114
TspGWI ACGGA 2 cut(s) 1123, 1526
TspRI CASTG 6 cut(s) 355, 643, 690, 729, 823, 1561
VpaK11BI GGWCC 3 cut(s) 170, 290, 596
XapI RAATTY 5 cut(s) 92, 476, 1037, 1355, 1400
XceI RCATGY 3 cut(s) 386, 664, 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 1575
XmnI GAANNNNTTC 1 cut(s) 1060
ZrmI AGTACT 1 cut(s) 943
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.