MD06G1230100.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017202
Physical Location & Seq
Reverse (-)
35977459 .. 35979856
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1230100.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGAGAGTTTGAGCCAGCATTATAGTGTATTAATGCTCATGCTAATCATACTTAACGGGGCTTTACCGTCTTGTTTCTCGCAAAAAACACGACGCTTTGAGTTTAATGTTGAGTGGAAGAATGTAACTCGTTTGTGCAACACAAAGCCACTTCTAACAGTGAATGGAGAGTTTCCAGGGCCAACCATTGCTGTCTATGAAGGTGATCATGTTGAGATTAAGGTCACTAATCACGTTGCCGAAAACACCACCATCCATTGGCATGGTGTGAAGCAACTAAGAACAGGATGGGCAGACGGCCCAGCTTATATCACACAATGTCCTATAAGAAGGGGCAAGACTTACACATACAAGTTCACCGTGGAATACCAAAGAGGCACTCTCTGGTGGCATGCTCATTATGCCTGGCAACGAGCTACCGTTTATGGCGCCTTTGTCATCCATCCGCGCATGCCTTTTCCATTTTCCGCTCCGATTCAAGAAGAATTCCCCATCATTTTTGGTAATTATACGAAAAAAAGTGAATGGTGGAGTTCAGATGTAGGTAAAGTGGAAGCTGAAATGAAGACTGGAGCTGGACCTAATAGTTCAGATGCTTATACCATCAATGGGTTGCCAGGACCACTATATCCTTGCTCCAACAAAGATACCTTCATCAAAACTGTGGAACATGGAAAAACCTACTTGCTTAGAATTATCAATGCAGCACTCAACGATGAGCTTTTCTTTGCTGTGGCTAATCACACACTAACTGTCGTGGAGATTGATGCCGTGTACACCAAACCATTCACAACCTCAGCGATCATGGTAGGTCCTGGCCAAACCACCAACGTTTTGCTCACTGCAAATCAAGTCCCTGACTCCTCAGGTGCCTTTTTGATGGCGGCCTGGCCTTACCTCACCTCTGTTTTCCCTTTCGACAATTCCACCACAGCAGGTTTCTTGCGTTACAAGAACAAAAAGGGTGACAAAACTAAGTATCCCCCGAAAGCGCCGAGTACTTTTGCACCGGAAAAATACAACCTCCCTAAAATGGAAGACACGAAGTTTGTCACTAAATTTAATAACAAGCTCAGAAGCCTAGCCTCAACCAAGTATCCGTGCAACGTGCCTAAAATCGTCGACAAGCATGTTGTAATAGTTATAAGCCTAAATCTCCAAGATTGTCCGGCCAACAAAACCTGCAAGGGTTATGCTGGCAAGAGATTTGCAGCTTCTATGAACAACCAGTCCTTCATTCGTCCCGCCTTGTCGATTTTAGAATCACATTACAAGAAGCTCAGAGACGCCGAGTACTCCACCGATTTCCCAGAGAAGCCTTTGAAGCCGTTTGATTACACTGGTGTGGATCCAGTTTCGGAGAACGTGAACCCGAAATTTGGCACTAAGATTATGGAAGTGGAGCACGGAACAAACTTAGAAATTGTGCTACAAGGTACAAGTTTTCTTAACACTGAGAACCATCCAATTCATGTGCATGGACACAACTTTTTTATTGTCGGTATGGGGTTTGGAAACTTCAATGCTACCAAGGATCCGGCAAAGTACAACCTCGTTGACCCTCCGGAGAGAAACACGGTGGCGGTTCCAACCGGAGGATGGGCGGCGATTCGATTCAAGGCCGACAATCCGGGAGTTTGGTTCATTCATTGTCACCTTGAAGAGCATACTTCTTGGGGTCTTGCCTCGGGGCTTATTGTGAAAAATGGTCCCAAACCGTCTCAGTGTGTGCTTCCTCCCCCTGACGATCTTCCCTCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

588

Amino Acids

65.73

Weight (kDa)

8.82

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 150 7.2e-45 Multicopper oxidase
Cu-oxidase PF00394 174 - 319 5.7e-39 Multicopper oxidase
Cu-oxidase_2 PF07731 436 - 569 2.1e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18140
fragaria_vesca FvH4_5g17150
malus_domestica MD06G1230100.v1.1 MD14G1234800.v1.1
prunus_persica Prupe.5G231100_v2.0.a1
pyrus_communis pycom14g19580
rosa_chinensis RchiOBHm_Chr7g0178641
rosa_laevigata RLG00000005427
rosa_multiflora Rmu_sc0002320.1_g000007
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017400 Rh7BG017000
rosa_wichuraiana Rw7G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1145
Acc36I ACCTGC 2 cut(s) 926, 1190
AccB1I GGYRCC 2 cut(s) 428, 869
AccB7I CCANNNNNTGG 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 1122
AccII CGCG 1 cut(s) 448
AccIII TCCGGA 1 cut(s) 1564
AciI CCGC 6 cut(s) 446, 468, 884, 1245, 1583, 1604
AclI AACGTT 1 cut(s) 831
AclWI GGATC 4 cut(s) 1343, 1356, 1529, 1542
AcoI YGGCCR 2 cut(s) 817, 1170
AcsI RAATTY 3 cut(s) 485, 1058, 1376
AcyI GRCGYC 2 cut(s) 429, 1287
AfaI GTAC 5 cut(s) 776, 1000, 1295, 1438, 1547
AfiI CCNNNNNNNGG 6 cut(s) 258, 609, 1033, 1379, 1595, 1599
AgsI TTSAA 5 cut(s) 479, 1324, 1522, 1618, 1661
AjnI CCWGG 5 cut(s) 175, 404, 616, 814, 887
AleI CACNNNNGTG 1 cut(s) 1343
AloI GAACNNNNNNTCC 2 cut(s) 1214, 1246
AluBI AGCT 8 cut(s) 305, 416, 557, 575, 721, 1072, 1214, 1279
AluI AGCT 8 cut(s) 305, 416, 557, 575, 721, 1072, 1214, 1279
Alw21I GWGCWC 1 cut(s) 1407
Alw26I GTCTC 2 cut(s) 1278, 1725
AlwI GGATC 4 cut(s) 1343, 1356, 1529, 1542
Ama87I CYCGRG 1 cut(s) 1687
Aor13HI TCCGGA 1 cut(s) 1564
AoxI GGCC 7 cut(s) 179, 298, 817, 885, 890, 1170, 1620
ApeKI GCWGC 2 cut(s) 704, 1211
ApoI RAATTY 3 cut(s) 485, 1058, 1376
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
AseI ATTAAT 1 cut(s) 32
AspLEI GCGC 3 cut(s) 431, 450, 994
AspS9I GGNCC 6 cut(s) 179, 299, 578, 620, 812, 1709
AsuC2I CCSGG 1 cut(s) 1632
AsuHPI GGTGA 5 cut(s) 215, 349, 892, 977, 1646
AvaI CYCGRG 1 cut(s) 1687
AvaII GGWCC 4 cut(s) 578, 620, 812, 1709
AxyI CCTNAGG 1 cut(s) 865
BalI TGGCCA 1 cut(s) 819
BamHI GGATCC 2 cut(s) 1348, 1534
BanI GGYRCC 2 cut(s) 428, 869
BbsI GAAGAC 2 cut(s) 572, 1044
Bbv12I GWGCWC 1 cut(s) 1407
BbvCI CCTCAGC 1 cut(s) 796
BbvI GCAGC 2 cut(s) 716, 1223
BccI CCATC 8 cut(s) 260, 282, 450, 500, 611, 874, 1470, 1593
BceAI ACGGC 3 cut(s) 313, 755, 1312
BciT130I CCWGG 5 cut(s) 177, 406, 618, 816, 889
BciVI GTATCC 2 cut(s) 990, 1107
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 1 cut(s) 1632
BcoDI GTCTC 2 cut(s) 1278, 1725
BfaI CTAG 1 cut(s) 1082
BfoI RGCGCY 2 cut(s) 432, 995
BfuAI ACCTGC 2 cut(s) 926, 1190
BfuI GTATCC 2 cut(s) 990, 1107
BisI GCNGC 4 cut(s) 705, 885, 1212, 1605
BlsI GCNGC 4 cut(s) 706, 886, 1213, 1606
BmcAI AGTACT 2 cut(s) 1000, 1295
Bme1390I CCNGG 6 cut(s) 177, 406, 618, 816, 889, 1632
Bme18I GGWCC 4 cut(s) 578, 620, 812, 1709
BmeT110I CYCGRG 1 cut(s) 1687
BmgT120I GGNCC 6 cut(s) 179, 299, 578, 620, 812, 1709
BmiI GGNNCC 6 cut(s) 430, 871, 1350, 1536, 1587, 1711
BmrFI CCNGG 6 cut(s) 177, 406, 618, 816, 889, 1632
BmsI GCATC 2 cut(s) 583, 757
BpiI GAAGAC 2 cut(s) 572, 1044
BplI GAGNNNNNCTC 2 cut(s) 366, 398
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 796
BpuMI CCSGG 1 cut(s) 1632
BsaHI GRCGYC 2 cut(s) 429, 1287
BsaJI CCNNGG 4 cut(s) 176, 360, 1530, 1686
BsaWI WCCGGW 3 cut(s) 1009, 1564, 1592
Bsc4I CCNNNNNNNGG 6 cut(s) 258, 609, 1033, 1379, 1595, 1599
Bse1I ACTGG 4 cut(s) 574, 1228, 1345, 1352
Bse21I CCTNAGG 1 cut(s) 865
Bse3DI GCAATG 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 1564
BseBI CCWGG 5 cut(s) 177, 406, 618, 816, 889
BseDI CCNNGG 4 cut(s) 176, 360, 1530, 1686
BseGI GGATG 6 cut(s) 252, 293, 438, 442, 1462, 1604
BseLI CCNNNNNNNGG 6 cut(s) 258, 609, 1033, 1379, 1595, 1599
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 6 cut(s) 810, 879, 1087, 1294, 1446, 1736
BseNI ACTGG 4 cut(s) 574, 1228, 1345, 1352
BseRI GAGGAG 1 cut(s) 853
BseXI GCAGC 2 cut(s) 716, 1223
BseYI CCCAGC 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 7 cut(s) 181, 300, 819, 887, 892, 1172, 1622
BshNI GGYRCC 2 cut(s) 428, 869
BsiHKAI GWGCWC 1 cut(s) 1407
BsiHKCI CYCGRG 1 cut(s) 1687
BsiSI CCGG 6 cut(s) 1010, 1169, 1538, 1565, 1593, 1631
BslFI GGGAC 3 cut(s) 839, 1227, 1695
BslI CCNNNNNNNGG 6 cut(s) 258, 609, 1033, 1379, 1595, 1599
BsmAI GTCTC 2 cut(s) 1278, 1725
BsmBI CGTCTC 2 cut(s) 1278, 1725
BsmFI GGGAC 3 cut(s) 839, 1227, 1695
BsnI GGCC 7 cut(s) 181, 300, 819, 887, 892, 1172, 1622
BsoBI CYCGRG 1 cut(s) 1687
Bsp1286I GDGCHC 1 cut(s) 1407
Bsp13I TCCGGA 1 cut(s) 1564
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 5 cut(s) 205, 801, 1348, 1534, 1747
BspACI CCGC 6 cut(s) 446, 468, 884, 1245, 1583, 1604
BspANI GGCC 7 cut(s) 181, 300, 819, 887, 892, 1172, 1622
BspCNI CTCAG 6 cut(s) 809, 878, 1086, 1293, 1447, 1735
BspEI TCCGGA 1 cut(s) 1564
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 6 cut(s) 430, 871, 1350, 1536, 1587, 1711
BspMI ACCTGC 2 cut(s) 926, 1190
BspPI GGATC 4 cut(s) 1343, 1356, 1529, 1542
BspQI GCTCTTC 1 cut(s) 1656
BspT107I GGYRCC 2 cut(s) 428, 869
BsrBI CCGCTC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 4 cut(s) 574, 1228, 1345, 1352
BssECI CCNNGG 4 cut(s) 176, 360, 1530, 1686
BssMI GATC 5 cut(s) 205, 801, 1348, 1534, 1747
BssNI GRCGYC 2 cut(s) 429, 1287
BssT1I CCWWGG 1 cut(s) 1530
Bst2UI CCWGG 5 cut(s) 177, 406, 618, 816, 889
Bst4CI ACNGT 8 cut(s) 69, 160, 361, 421, 664, 754, 1579, 1719
Bst6I CTCTTC 1 cut(s) 1656
BstACI GRCGYC 2 cut(s) 429, 1287
BstAUI TGTACA 1 cut(s) 774
BstC8I GCNNGC 4 cut(s) 17, 393, 452, 1198
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 6 cut(s) 252, 293, 438, 442, 1462, 1604
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 2 cut(s) 432, 995
BstHHI GCGC 3 cut(s) 431, 450, 994
BstKTI GATC 5 cut(s) 208, 804, 1351, 1537, 1750
BstMAI GTCTC 2 cut(s) 1278, 1725
BstMBI GATC 5 cut(s) 205, 801, 1348, 1534, 1747
BstMWI GCNNNNNNNGC 2 cut(s) 401, 1324
BstNI CCWGG 5 cut(s) 177, 406, 618, 816, 889
BstNSI RCATGY 3 cut(s) 395, 454, 1133
BstSCI CCNGG 6 cut(s) 175, 404, 616, 814, 887, 1630
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 716, 1223
BstV2I GAAGAC 2 cut(s) 572, 1044
BstX2I RGATCY 2 cut(s) 1348, 1534
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 2 cut(s) 1348, 1534
Bsu36I CCTNAGG 1 cut(s) 865
BsuI GTATCC 2 cut(s) 990, 1107
BsuRI GGCC 7 cut(s) 181, 300, 819, 887, 892, 1172, 1622
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 6 cut(s) 252, 293, 438, 442, 1462, 1604
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 5 cut(s) 165, 840, 1338, 1452, 1730
BveI ACCTGC 2 cut(s) 926, 1190
Cac8I GCNNGC 4 cut(s) 17, 393, 452, 1198
CfoI GCGC 3 cut(s) 431, 450, 994
Cfr13I GGNCC 6 cut(s) 179, 299, 578, 620, 812, 1709
CseI GACGC 2 cut(s) 102, 1295
Csp6I GTAC 5 cut(s) 775, 999, 1294, 1437, 1546
CviQI GTAC 5 cut(s) 775, 999, 1294, 1437, 1546
DinI GGCGCC 1 cut(s) 430
DpnI GATC 5 cut(s) 207, 803, 1350, 1536, 1749
DpnII GATC 5 cut(s) 205, 801, 1348, 1534, 1747
EaeI YGGCCR 2 cut(s) 817, 1170
Eam1104I CTCTTC 1 cut(s) 1656
EarI CTCTTC 1 cut(s) 1656
Eco130I CCWWGG 1 cut(s) 1530
Eco47I GGWCC 4 cut(s) 578, 620, 812, 1709
Eco81I CCTNAGG 1 cut(s) 865
Eco88I CYCGRG 1 cut(s) 1687
EcoO109I RGGNCCY 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 485
EcoRII CCWGG 5 cut(s) 175, 404, 616, 814, 887
EcoT14I CCWWGG 1 cut(s) 1530
EgeI GGCGCC 1 cut(s) 430
EheI GGCGCC 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 1530
Esp3I CGTCTC 2 cut(s) 1278, 1725
FaqI GGGAC 3 cut(s) 839, 1227, 1695
FauI CCCGC 1 cut(s) 1252
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 1122
Fnu4HI GCNGC 4 cut(s) 705, 885, 1212, 1605
FokI GGATG 6 cut(s) 239, 300, 425, 429, 1449, 1611
Fsp4HI GCNGC 4 cut(s) 705, 885, 1212, 1605
FspBI CTAG 1 cut(s) 1082
GlaI GCGC 3 cut(s) 430, 449, 993
GluI GCNGC 4 cut(s) 705, 885, 1212, 1605
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 2 cut(s) 432, 995
HaeIII GGCC 7 cut(s) 181, 300, 819, 887, 892, 1172, 1622
HapII CCGG 6 cut(s) 1010, 1169, 1538, 1565, 1593, 1631
HgaI GACGC 2 cut(s) 102, 1295
HhaI GCGC 3 cut(s) 431, 450, 994
Hin1I GRCGYC 2 cut(s) 429, 1287
Hin6I GCGC 3 cut(s) 429, 448, 992
HinP1I GCGC 3 cut(s) 429, 448, 992
HincII GTYRAC 2 cut(s) 1123, 1558
HindII GTYRAC 2 cut(s) 1123, 1558
HinfI GANTC 5 cut(s) 475, 860, 1262, 1609, 1614
HpaII CCGG 6 cut(s) 1010, 1169, 1538, 1565, 1593, 1631
HphI GGTGA 5 cut(s) 215, 349, 892, 977, 1646
Hpy166II GTNNAC 6 cut(s) 357, 775, 777, 1123, 1369, 1558
Hpy188I TCNGA 6 cut(s) 474, 538, 592, 1076, 1283, 1360
Hpy188III TCNNGA 2 cut(s) 479, 1565
Hpy8I GTNNAC 6 cut(s) 357, 775, 777, 1123, 1369, 1558
Hpy99I CGWCG 2 cut(s) 96, 1124
HpyAV CCTTC 4 cut(s) 194, 324, 661, 1243
HpyCH4III ACNGT 8 cut(s) 69, 160, 361, 421, 664, 754, 1579, 1719
HpyCH4IV ACGT 4 cut(s) 234, 831, 1107, 1365
HpyCH4V TGCA 8 cut(s) 138, 704, 845, 1007, 1104, 1185, 1211, 1477
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 1324
HpySE526I ACGT 4 cut(s) 234, 831, 1107, 1365
Hsp92I GRCGYC 2 cut(s) 429, 1287
HspAI GCGC 3 cut(s) 429, 448, 992
KasI GGCGCC 1 cut(s) 428
Kpn2I TCCGGA 1 cut(s) 1564
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 5 cut(s) 205, 801, 1348, 1534, 1747
LguI GCTCTTC 1 cut(s) 1656
LmnI GCTCC 4 cut(s) 475, 572, 641, 1402
Lsp1109I GCAGC 2 cut(s) 716, 1223
LweI GCATC 2 cut(s) 583, 757
MaeI CTAG 1 cut(s) 1082
MaeII ACGT 4 cut(s) 234, 831, 1107, 1365
MaeIII GTNAC 6 cut(s) 124, 223, 947, 965, 1051, 1652
MalI GATC 5 cut(s) 207, 803, 1350, 1536, 1749
MbiI CCGCTC 1 cut(s) 470
MboI GATC 5 cut(s) 205, 801, 1348, 1534, 1747
MboII GAAGA 6 cut(s) 130, 494, 577, 1049, 1673, 1742
MflI RGATCY 2 cut(s) 1348, 1534
MhlI GDGCHC 1 cut(s) 1407
MlsI TGGCCA 1 cut(s) 819
MluCI AATT 8 cut(s) 485, 505, 693, 922, 1058, 1376, 1422, 1467
MluNI TGGCCA 1 cut(s) 819
Mly113I GGCGCC 1 cut(s) 429
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 2 cut(s) 663, 1613
Mox20I TGGCCA 1 cut(s) 819
MroI TCCGGA 1 cut(s) 1564
MscI TGGCCA 1 cut(s) 819
MseI TTAA 6 cut(s) 32, 54, 105, 219, 1062, 1449
MslI CAYNNNNRTG 4 cut(s) 24, 261, 1343, 1476
Msp20I TGGCCA 1 cut(s) 819
MspI CCGG 6 cut(s) 1010, 1169, 1538, 1565, 1593, 1631
MspR9I CCNGG 6 cut(s) 177, 406, 618, 816, 889, 1632
MvaI CCWGG 5 cut(s) 177, 406, 618, 816, 889
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 2 cut(s) 401, 1324
NarI GGCGCC 1 cut(s) 429
NciI CCSGG 1 cut(s) 1632
NdeII GATC 5 cut(s) 205, 801, 1348, 1534, 1747
NlaIV GGNNCC 6 cut(s) 430, 871, 1350, 1536, 1587, 1711
NmeAIII GCCGAG 2 cut(s) 1020, 1315
NmuCI GTSAC 4 cut(s) 223, 965, 1051, 1652
NspI RCATGY 3 cut(s) 395, 454, 1133
OliI CACNNNNGTG 1 cut(s) 1343
PaeI GCATGC 2 cut(s) 395, 454
PciSI GCTCTTC 1 cut(s) 1656
PfeI GAWTC 4 cut(s) 475, 1262, 1609, 1614
PflMI CCANNNNNTGG 1 cut(s) 258
PfoI TCCNGGA 1 cut(s) 1630
PkrI GCNGC 4 cut(s) 706, 886, 1213, 1606
PleI GAGTC 1 cut(s) 854
PluTI GGCGCC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 812
PshBI ATTAAT 1 cut(s) 32
PsiI TTATAA 1 cut(s) 1145
Psp1406I AACGTT 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 5 cut(s) 175, 404, 616, 814, 887
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 5 cut(s) 175, 404, 616, 814, 887
PspN4I GGNNCC 6 cut(s) 430, 871, 1350, 1536, 1587, 1711
PspPI GGNCC 6 cut(s) 179, 299, 578, 620, 812, 1709
PspPPI RGGWCCY 1 cut(s) 812
PsuI RGATCY 2 cut(s) 1348, 1534
RsaI GTAC 5 cut(s) 776, 1000, 1295, 1438, 1547
RsaNI GTAC 5 cut(s) 775, 999, 1294, 1437, 1546
RseI CAYNNNNRTG 4 cut(s) 24, 261, 1343, 1476
SalI GTCGAC 1 cut(s) 1121
SapI GCTCTTC 1 cut(s) 1656
SaqAI TTAA 6 cut(s) 32, 54, 105, 219, 1062, 1449
SatI GCNGC 4 cut(s) 705, 885, 1212, 1605
Sau3AI GATC 5 cut(s) 205, 801, 1348, 1534, 1747
Sau96I GGNCC 6 cut(s) 179, 299, 578, 620, 812, 1709
ScaI AGTACT 2 cut(s) 1000, 1295
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 6 cut(s) 177, 406, 618, 816, 889, 1632
SduI GDGCHC 1 cut(s) 1407
SfaNI GCATC 2 cut(s) 583, 757
SfoI GGCGCC 1 cut(s) 430
SinI GGWCC 4 cut(s) 578, 620, 812, 1709
SmiMI CAYNNNNRTG 4 cut(s) 24, 261, 1343, 1476
SphI GCATGC 2 cut(s) 395, 454
Sse9I AATT 8 cut(s) 485, 505, 693, 922, 1058, 1376, 1422, 1467
SsiI CCGC 6 cut(s) 446, 468, 884, 1245, 1583, 1604
SspDI GGCGCC 1 cut(s) 428
SspMI CTAG 1 cut(s) 1082
StyD4I CCNGG 6 cut(s) 175, 404, 616, 814, 887, 1630
StyI CCWWGG 1 cut(s) 1530
TaaI ACNGT 8 cut(s) 69, 160, 361, 421, 664, 754, 1579, 1719
TaiI ACGT 4 cut(s) 237, 834, 1110, 1368
TaqI TCGA 4 cut(s) 918, 1122, 1253, 1612
TasI AATT 8 cut(s) 485, 505, 693, 922, 1058, 1376, 1422, 1467
TatI WGTACW 4 cut(s) 774, 998, 1293, 1545
TauI GCSGC 2 cut(s) 887, 1607
TfiI GAWTC 4 cut(s) 475, 1262, 1609, 1614
Tru1I TTAA 6 cut(s) 32, 54, 105, 219, 1062, 1449
Tru9I TTAA 6 cut(s) 32, 54, 105, 219, 1062, 1449
TscAI CASTG 5 cut(s) 165, 847, 1345, 1459, 1730
TseFI GTSAC 4 cut(s) 223, 965, 1051, 1652
TseI GCWGC 2 cut(s) 704, 1211
Tsp45I GTSAC 4 cut(s) 223, 965, 1051, 1652
TspDTI ATGAA 8 cut(s) 213, 578, 643, 1225, 1235, 1460, 1633, 1637
TspGWI ACGGA 2 cut(s) 1089, 1422
TspRI CASTG 5 cut(s) 165, 847, 1345, 1459, 1730
Van91I CCANNNNNTGG 1 cut(s) 258
VpaK11BI GGWCC 4 cut(s) 578, 620, 812, 1709
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 3 cut(s) 485, 1058, 1376
XceI RCATGY 3 cut(s) 395, 454, 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1596
XmiI GTMKAC 1 cut(s) 1122
XspI CTAG 1 cut(s) 1082
ZrmI AGTACT 2 cut(s) 1000, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.