Rw7G001410

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0017202
Physical Location & Seq
Forward (+)
1150206 .. 1152495
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001410.1

Sequence Viewer

Length: 1632 bp
ATGGAGGGTTTTTGCCAGTTAGTATTGGTAACTCTGTTGATCATAGCTGGGCTTCTACCATCTTGTGTTTCACAAACAACCCGGCGCTTTGAATTTAATGTTGAGTGGAAAAATGTGACCCGTTTGTGCCACACGAAGCCACTTCTAACTGTGAATGGAGAGTATCCAGGTCCAACGATTGCAGTTCATGAAGGTGATAATGTAGAAATTAAGGTGACTAATCGCATTGCGCAGAACACTACCCTCCATTGGCATGGCATAAGGCAACTAAGAACAGGATGGGCACGGTCCAGGAAGACCTACACATACAAGTTCACTGTAATCAACCAAAGAGGCACCCTTTGGTGGCATTCTCATCATTCGTGGCAACGAGCTTCTGTTTATGGCGCTTTTATCATCCACCCTCGCATGCCATATCCATTTTCAGTTCCAATTCAAGATGAATTCCCCATCATCTTTGGTGAATGGTGGAATGGAGATATAGATTCAGTCGAAAGTGAAATGATGAAGTATGGAGGGGGCCCTGATATTTCAAATGCATATACCATAAACGGTTTGCCAGGACCATTTTATAATTGCTCGAACAAAGATACATTGATCAACACTGTGGAACATGGCAAAACATACATGCTAAGAATCATCAATGCAGCACTGAATGATGAACTATTCTTTGCTGTTGCTAATCACACACTGACGGTTGTGGAGATTGATGCAGCGTACACAAAACCATTCAAAACCAAAGCCATTATGATAGCTCCTGGCCAAACTACCAATGTTCTGCTCACTGCTAATCAAGTGCCTGATTCCTCCGGCATGTTCTTAATGGCTGCCTGGACTTACCTCACATCTCACCCGAAAAAGGTTACTACTTTTGATCCTGTCATGTACAACTTTCCTCAGATGGATGATACCAAATATTACACTAAATTTGATGACAATCTGAGAAGCCTTTCCTCCCCTCGGTATCCATGTAATGTGCCAAAAACTATCCACAAACGGATAGTGACAGTTATAAGCCTTAACCTCCAAGATTGTCCTGCTAACAAAACCTGCAAGGGCTATGCCAACAAGAGCTTCTATGCTTCAATGAACAATCAGTCCTTTGTTCGTCCCGCCGTGTCAATATTGGAATCTCATTACAAGAAGCTCAAAAACAGTCAATACTCCACCGATTTCCCAGAGAAGCCTCGAAGGCCATTTGACTATACCGGGGTGGATCCAGTCACCGAAAACATGAACACAGAATTTGGTACAAAGATCATACAAGTCCCCTATGGAACAAACTTGGAATTTGTGCTGCAAGGTACAAGCTTCCTTAATGTGGAGAACCACCCTATTCATTTTCATGGACACAACTTCTTTATAGTTGGTAAGGGGTTTGGGAACTTCAATGTCTCCACGGATCCAGCAAAGTACAACCTTGTTGATCCTCCTGAGAGAAACACTGTGGCAGTTCCAAGTGGAGGATGGGCAGCGATTCGGTTCAAGGCCGATAATCCAGGAGTGTGGTTCATACATTGTCATCTTGAAGAACACACTTCTTGGGGTCTTGCCTCAGGCTTCATAGTTCAAAATGGTCATGGACCATCTCAATCCTTGCTTCCTCCTCCTAAAGATCTTCCCTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

543

Amino Acids

61.4

Weight (kDa)

8.55

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 136 1.6e-30 Multicopper oxidase
Cu-oxidase PF00394 148 - 278 2.7e-37 Multicopper oxidase
Cu-oxidase_2 PF07731 392 - 526 2.9e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18140
fragaria_vesca FvH4_5g17150
malus_domestica MD06G1230100.v1.1 MD14G1234800.v1.1
prunus_persica Prupe.5G231100_v2.0.a1
pyrus_communis pycom14g19580
rosa_chinensis RchiOBHm_Chr7g0178641
rosa_laevigata RLG00000005427
rosa_multiflora Rmu_sc0002320.1_g000007
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017400 Rh7BG017000
rosa_wichuraiana Rw7G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 573, 1013
Acc16I TGCGCA 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 1058
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 1 cut(s) 1113
AclWI GGATC 6 cut(s) 869, 1211, 1224, 1397, 1410, 1421
AcoI YGGCCR 1 cut(s) 760
AcsI RAATTY 5 cut(s) 92, 443, 926, 1244, 1289
AfaI GTAC 5 cut(s) 719, 887, 1252, 1306, 1415
AfiI CCNNNNNNNGG 6 cut(s) 249, 345, 859, 960, 1321, 1463
AgsI TTSAA 9 cut(s) 92, 437, 534, 733, 1086, 1390, 1486, 1529, 1571
AjnI CCWGG 6 cut(s) 166, 290, 559, 757, 830, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
AloI GAACNNNNNNTCC 2 cut(s) 1082, 1114
AluBI AGCT 6 cut(s) 47, 374, 755, 1074, 1147, 1311
AluI AGCT 6 cut(s) 47, 374, 755, 1074, 1147, 1311
Alw26I GTCTC 1 cut(s) 1399
AlwI GGATC 6 cut(s) 869, 1211, 1224, 1397, 1410, 1421
AoxI GGCC 4 cut(s) 520, 760, 1193, 1488
ApaI GGGCCC 1 cut(s) 524
ApeKI GCWGC 5 cut(s) 647, 713, 827, 1297, 1472
ApoI RAATTY 5 cut(s) 92, 443, 926, 1244, 1289
Asp700I GAANNNNTTC 1 cut(s) 949
AspLEI GCGC 3 cut(s) 87, 232, 389
AspS9I GGNCC 6 cut(s) 170, 288, 520, 521, 563, 1583
AsuC2I CCSGG 2 cut(s) 82, 1210
AsuHPI GGTGA 5 cut(s) 206, 226, 473, 842, 1216
AvaII GGWCC 4 cut(s) 170, 288, 563, 1583
AxyI CCTNAGG 1 cut(s) 1555
BaeGI GKGCMC 2 cut(s) 286, 524
BalI TGGCCA 1 cut(s) 762
BamHI GGATCC 2 cut(s) 1216, 1402
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 302
BbvI GCAGC 5 cut(s) 659, 725, 814, 1284, 1484
BccI CCATC 6 cut(s) 67, 273, 458, 895, 1461, 1594
BceAI ACGGC 1 cut(s) 1100
BciT130I CCWGG 6 cut(s) 168, 292, 561, 759, 832, 1500
BciVI GTATCC 2 cut(s) 174, 975
BclI TGATCA 2 cut(s) 39, 597
BcnI CCSGG 2 cut(s) 82, 1210
BcoDI GTCTC 1 cut(s) 1399
BfoI RGCGCY 2 cut(s) 88, 390
BfuAI ACCTGC 1 cut(s) 1058
BfuI GTATCC 2 cut(s) 174, 975
BglI GCCNNNNNGGC 1 cut(s) 1192
BglII AGATCT 1 cut(s) 1615
BisI GCNGC 5 cut(s) 648, 714, 828, 1298, 1473
BlsI GCNGC 5 cut(s) 649, 715, 829, 1299, 1474
Bme1390I CCNGG 8 cut(s) 82, 168, 292, 561, 759, 832, 1210, 1500
Bme18I GGWCC 4 cut(s) 170, 288, 563, 1583
BmgT120I GGNCC 6 cut(s) 170, 288, 520, 521, 563, 1583
BmiI GGNNCC 5 cut(s) 337, 521, 522, 1218, 1404
BmrFI CCNGG 8 cut(s) 82, 168, 292, 561, 759, 832, 1210, 1500
BmsI GCATC 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 302
BpuMI CCSGG 2 cut(s) 82, 1210
BsaBI GATNNNNATC 1 cut(s) 936
BsaJI CCNNGG 3 cut(s) 959, 1209, 1398
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 345, 859, 960, 1321, 1463
Bse1I ACTGG 2 cut(s) 16, 1220
Bse21I CCTNAGG 1 cut(s) 1555
Bse3DI GCAATG 1 cut(s) 225
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 6 cut(s) 168, 292, 561, 759, 832, 1500
BseDI CCNNGG 3 cut(s) 959, 1209, 1398
BseGI GGATG 4 cut(s) 284, 396, 910, 1472
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 6 cut(s) 249, 345, 859, 960, 1321, 1463
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 4 cut(s) 911, 932, 1425, 1569
BseNI ACTGG 2 cut(s) 16, 1220
BseRI GAGGAG 1 cut(s) 1596
BseSI GKGCMC 2 cut(s) 286, 524
BseXI GCAGC 5 cut(s) 659, 725, 814, 1284, 1484
BseYI CCCAGC 1 cut(s) 47
BshFI GGCC 4 cut(s) 522, 762, 1195, 1490
BshNI GGYRCC 1 cut(s) 335
BsiSI CCGG 3 cut(s) 82, 810, 1209
BslFI GGGAC 2 cut(s) 1095, 1253
BslI CCNNNNNNNGG 6 cut(s) 249, 345, 859, 960, 1321, 1463
BsmAI GTCTC 1 cut(s) 1399
BsmFI GGGAC 2 cut(s) 1095, 1253
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 4 cut(s) 522, 762, 1195, 1490
Bsp120I GGGCCC 1 cut(s) 520
Bsp1286I GDGCHC 2 cut(s) 286, 524
Bsp1407I TGTACA 1 cut(s) 885
Bsp143I GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
BspACI CCGC 1 cut(s) 1113
BspANI GGCC 4 cut(s) 522, 762, 1195, 1490
BspCNI CTCAG 4 cut(s) 910, 933, 1426, 1568
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 5 cut(s) 337, 521, 522, 1218, 1404
BspMI ACCTGC 1 cut(s) 1058
BspPI GGATC 6 cut(s) 869, 1211, 1224, 1397, 1410, 1421
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 225
BsrGI TGTACA 1 cut(s) 885
BsrI ACTGG 2 cut(s) 16, 1220
BssECI CCNNGG 3 cut(s) 959, 1209, 1398
BssMI GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
Bst2UI CCWGG 6 cut(s) 168, 292, 561, 759, 832, 1500
Bst4CI ACNGT 9 cut(s) 151, 288, 319, 554, 607, 697, 1009, 1157, 1447
BstAUI TGTACA 1 cut(s) 885
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 6 cut(s) 269, 632, 897, 941, 1434, 1555
BstDSI CCRYGG 1 cut(s) 1398
BstF5I GGATG 4 cut(s) 284, 396, 910, 1472
BstH2I RGCGCY 2 cut(s) 88, 390
BstHHI GCGC 3 cut(s) 87, 232, 389
BstKTI GATC 8 cut(s) 42, 600, 877, 1219, 1260, 1405, 1429, 1618
BstMAI GTCTC 1 cut(s) 1399
BstMBI GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
BstMWI GCNNNNNNNGC 1 cut(s) 1192
BstNI CCWGG 6 cut(s) 168, 292, 561, 759, 832, 1500
BstNSI RCATGY 3 cut(s) 412, 631, 817
BstSCI CCNGG 8 cut(s) 80, 166, 290, 559, 757, 830, 1208, 1498
BstSLI GKGCMC 2 cut(s) 286, 524
BstV1I GCAGC 5 cut(s) 659, 725, 814, 1284, 1484
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 3 cut(s) 1216, 1402, 1615
BstXI CCANNNNNNTGG 2 cut(s) 254, 1506
BstYI RGATCY 3 cut(s) 1216, 1402, 1615
Bsu36I CCTNAGG 1 cut(s) 1555
BsuI GTATCC 2 cut(s) 174, 975
BsuRI GGCC 4 cut(s) 522, 762, 1195, 1490
BtgI CCRYGG 1 cut(s) 1398
BtsCI GGATG 4 cut(s) 284, 396, 910, 1472
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 6 cut(s) 315, 603, 650, 689, 783, 1443
BveI ACCTGC 1 cut(s) 1058
Cac8I GCNNGC 1 cut(s) 410
CciI TCATGA 1 cut(s) 187
CfoI GCGC 3 cut(s) 87, 232, 389
Cfr13I GGNCC 6 cut(s) 170, 288, 520, 521, 563, 1583
Csp6I GTAC 5 cut(s) 718, 886, 1251, 1305, 1414
CviQI GTAC 5 cut(s) 718, 886, 1251, 1305, 1414
DdeI CTNAG 6 cut(s) 269, 632, 897, 941, 1434, 1555
DpnI GATC 8 cut(s) 41, 599, 876, 1218, 1259, 1404, 1428, 1617
DpnII GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
EaeI YGGCCR 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 524
Eco47I GGWCC 4 cut(s) 170, 288, 563, 1583
Eco81I CCTNAGG 1 cut(s) 1555
EcoO109I RGGNCCY 2 cut(s) 520, 521
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 6 cut(s) 166, 290, 559, 757, 830, 1498
EcoT22I ATGCAT 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 524
FaqI GGGAC 2 cut(s) 1095, 1253
FauI CCCGC 1 cut(s) 1120
FbaI TGATCA 2 cut(s) 39, 597
Fnu4HI GCNGC 5 cut(s) 648, 714, 828, 1298, 1473
FokI GGATG 4 cut(s) 291, 383, 917, 1479
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 5 cut(s) 648, 714, 828, 1298, 1473
FspI TGCGCA 1 cut(s) 231
GlaI GCGC 3 cut(s) 86, 231, 388
GluI GCNGC 5 cut(s) 648, 714, 828, 1298, 1473
GsaI CCCAGC 1 cut(s) 51
HaeII RGCGCY 2 cut(s) 88, 390
HaeIII GGCC 4 cut(s) 522, 762, 1195, 1490
HapII CCGG 3 cut(s) 82, 810, 1209
HhaI GCGC 3 cut(s) 87, 232, 389
Hin6I GCGC 3 cut(s) 85, 230, 387
HinP1I GCGC 3 cut(s) 85, 230, 387
HindIII AAGCTT 1 cut(s) 1309
HinfI GANTC 5 cut(s) 485, 636, 803, 1130, 1477
HpaII CCGG 3 cut(s) 82, 810, 1209
HphI GGTGA 5 cut(s) 206, 226, 473, 842, 1216
Hpy166II GTNNAC 2 cut(s) 315, 720
Hpy188I TCNGA 2 cut(s) 900, 942
Hpy188III TCNNGA 4 cut(s) 188, 437, 1433, 1526
Hpy8I GTNNAC 2 cut(s) 315, 720
HpyAV CCTTC 2 cut(s) 185, 1185
HpyCH4III ACNGT 9 cut(s) 151, 288, 319, 554, 607, 697, 1009, 1157, 1447
HpyCH4V TGCA 6 cut(s) 182, 539, 647, 713, 1053, 1300
HpyF10VI GCNNNNNNNGC 1 cut(s) 1192
HpyF3I CTNAG 6 cut(s) 269, 632, 897, 941, 1434, 1555
HspAI GCGC 3 cut(s) 85, 230, 387
Ksp22I TGATCA 2 cut(s) 39, 597
Kzo9I GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
LmnI GCTCC 1 cut(s) 760
Lsp1109I GCAGC 5 cut(s) 659, 725, 814, 1284, 1484
LweI GCATC 1 cut(s) 700
MaeIII GTNAC 6 cut(s) 28, 115, 214, 862, 1003, 1222
MalI GATC 8 cut(s) 41, 599, 876, 1218, 1259, 1404, 1428, 1617
MboI GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
MboII GAAGA 3 cut(s) 307, 1541, 1610
MflI RGATCY 3 cut(s) 1216, 1402, 1615
MhlI GDGCHC 2 cut(s) 286, 524
MlsI TGGCCA 1 cut(s) 762
MluCI AATT 8 cut(s) 92, 207, 432, 443, 574, 926, 1244, 1289
MluNI TGGCCA 1 cut(s) 762
MmeI TCCRAC 1 cut(s) 197
Mox20I TGGCCA 1 cut(s) 762
Mph1103I ATGCAT 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 949
MscI TGGCCA 1 cut(s) 762
MseI TTAA 5 cut(s) 96, 210, 821, 1020, 1317
MslI CAYNNNNRTG 3 cut(s) 192, 252, 1344
Msp20I TGGCCA 1 cut(s) 762
MspI CCGG 3 cut(s) 82, 810, 1209
MspR9I CCNGG 8 cut(s) 82, 168, 292, 561, 759, 832, 1210, 1500
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 6 cut(s) 168, 292, 561, 759, 832, 1500
MwoI GCNNNNNNNGC 1 cut(s) 1192
NciI CCSGG 2 cut(s) 82, 1210
NdeII GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
NlaIV GGNNCC 5 cut(s) 337, 521, 522, 1218, 1404
NmuCI GTSAC 4 cut(s) 115, 214, 1003, 1222
NsbI TGCGCA 1 cut(s) 231
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 3 cut(s) 412, 631, 817
PaeI GCATGC 1 cut(s) 412
PagI TCATGA 1 cut(s) 187
PctI GAATGC 1 cut(s) 349
PdmI GAANNNNTTC 1 cut(s) 949
PfeI GAWTC 5 cut(s) 485, 636, 803, 1130, 1477
PfoI TCCNGGA 2 cut(s) 290, 1498
PkrI GCNGC 5 cut(s) 649, 715, 829, 1299, 1474
PsiI TTATAA 2 cut(s) 573, 1013
Psp6I CCWGG 6 cut(s) 166, 290, 559, 757, 830, 1498
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 6 cut(s) 166, 290, 559, 757, 830, 1498
PspN4I GGNNCC 5 cut(s) 337, 521, 522, 1218, 1404
PspOMI GGGCCC 1 cut(s) 520
PspPI GGNCC 6 cut(s) 170, 288, 520, 521, 563, 1583
PsuI RGATCY 3 cut(s) 1216, 1402, 1615
RsaI GTAC 5 cut(s) 719, 887, 1252, 1306, 1415
RsaNI GTAC 5 cut(s) 718, 886, 1251, 1305, 1414
RseI CAYNNNNRTG 3 cut(s) 192, 252, 1344
SaqAI TTAA 5 cut(s) 96, 210, 821, 1020, 1317
SatI GCNGC 5 cut(s) 648, 714, 828, 1298, 1473
Sau3AI GATC 8 cut(s) 39, 597, 874, 1216, 1257, 1402, 1426, 1615
Sau96I GGNCC 6 cut(s) 170, 288, 520, 521, 563, 1583
ScrFI CCNGG 8 cut(s) 82, 168, 292, 561, 759, 832, 1210, 1500
SduI GDGCHC 2 cut(s) 286, 524
SfaNI GCATC 1 cut(s) 700
SinI GGWCC 4 cut(s) 170, 288, 563, 1583
SmiMI CAYNNNNRTG 3 cut(s) 192, 252, 1344
SphI GCATGC 1 cut(s) 412
Sse9I AATT 8 cut(s) 92, 207, 432, 443, 574, 926, 1244, 1289
SsiI CCGC 1 cut(s) 1113
SspI AATATT 2 cut(s) 917, 1125
StyD4I CCNGG 8 cut(s) 80, 166, 290, 559, 757, 830, 1208, 1498
TaaI ACNGT 9 cut(s) 151, 288, 319, 554, 607, 697, 1009, 1157, 1447
TaqI TCGA 3 cut(s) 492, 581, 1189
TasI AATT 8 cut(s) 92, 207, 432, 443, 574, 926, 1244, 1289
TatI WGTACW 2 cut(s) 885, 1413
TfiI GAWTC 5 cut(s) 485, 636, 803, 1130, 1477
Tru1I TTAA 5 cut(s) 96, 210, 821, 1020, 1317
Tru9I TTAA 5 cut(s) 96, 210, 821, 1020, 1317
TscAI CASTG 6 cut(s) 322, 610, 657, 696, 790, 1450
TseFI GTSAC 4 cut(s) 115, 214, 1003, 1222
TseI GCWGC 5 cut(s) 647, 713, 827, 1297, 1472
Tsp45I GTSAC 4 cut(s) 115, 214, 1003, 1222
TspGWI ACGGA 2 cut(s) 1012, 1415
TspRI CASTG 6 cut(s) 322, 610, 657, 696, 790, 1450
VpaK11BI GGWCC 4 cut(s) 170, 288, 563, 1583
XapI RAATTY 5 cut(s) 92, 443, 926, 1244, 1289
XceI RCATGY 3 cut(s) 412, 631, 817
XcmI CCANNNNNNNNNTGG 1 cut(s) 1464
XmnI GAANNNNTTC 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.