FvH4_5g18310

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016082
Physical Location & Seq
Reverse (-)
10537280 .. 10537603
324 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18310.t1

Sequence Viewer

Length: 324 bp
ATGGGTTCAAGAGGAGGCGCGACGGTTTTGCAGCAGCTAAATCGGGCGAGTCGAATGAGAACGAAGCTTCAGACGGCGCTGGAAGCCAGCGCTCTGGAAATAGAAGACGTGTCACATCAGCACGCCGGACATGCCGCCATGAGGAATAACAACAACGCCGGCGAGACTCATTTCAACCTCAGGATCGTCTCCCCTAAATTCGAGGGCCAGAGCCTCGTTAAACGACACCGCATGGTTTACGACGCCTTGGCCGACGAGCTCCAGTCCGGTCTTCACGCCCTCTCCATCGTCGCCAAGACTCCCCAAGAATCCGGCCCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

108

Amino Acids

11.68

Weight (kDa)

9.69

Isoelectric Point (pI)

52.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 23 - 103 1.7e-32 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55805
fragaria_vesca FvH4_5g18310
malus_domestica MD06G1238900.v1.1
prunus_persica Prupe.5G242800_v2.0.a1
pyrus_communis pycom06g21450
rosa_chinensis RchiOBHm_Chr7g0177271
rosa_laevigata RLG00000005550
rosa_multiflora Rmu_sc0005310.1_g000011
rosa_roxburghii Rroxscaffold_3G00276000
rosa_rugosa Rorug06G0404300
rosa_samantha Rh7AG004300 Rh7BG004100 Rh7CG004000 Rh7DG004000
rosa_wichuraiana Rw7G000350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 135, 229
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 243
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 141
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 2 cut(s) 9, 175
AjiI CACGTC 1 cut(s) 109
AluBI AGCT 3 cut(s) 37, 67, 259
AluI AGCT 3 cut(s) 37, 67, 259
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 158, 193
AlwI GGATC 1 cut(s) 191
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 3 cut(s) 205, 249, 313
ApeKI GCWGC 2 cut(s) 31, 34
ApoI RAATTY 1 cut(s) 197
AspLEI GCGC 3 cut(s) 20, 79, 92
AspS9I GGNCC 2 cut(s) 205, 314
AxyI CCTNAGG 1 cut(s) 179
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 111, 263
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 43, 46
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 90
BcgI CGANNNNNNTGC 4 cut(s) 10, 23, 44, 57
BcoDI GTCTC 2 cut(s) 158, 193
BfoI RGCGCY 2 cut(s) 80, 93
BisI GCNGC 3 cut(s) 32, 35, 135
BlsI GCNGC 3 cut(s) 33, 36, 136
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 205, 314
BmiI GGNNCC 1 cut(s) 316
BpiI GAAGAC 2 cut(s) 111, 263
BpmI CTGGAG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 246
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 246
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 43, 46
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 3 cut(s) 207, 251, 315
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 4 cut(s) 126, 159, 267, 312
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 2 cut(s) 158, 193
BsmBI CGTCTC 1 cut(s) 193
BsnI GGCC 3 cut(s) 207, 251, 315
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 2 cut(s) 135, 229
BspANI GGCC 3 cut(s) 207, 251, 315
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 316
BspPI GGATC 1 cut(s) 191
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 1 cut(s) 183
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 243
BstC8I GCNNGC 3 cut(s) 88, 123, 160
BstDEI CTNAG 1 cut(s) 179
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 2 cut(s) 80, 93
BstHHI GCGC 3 cut(s) 20, 79, 92
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 2 cut(s) 158, 193
BstMBI GATC 1 cut(s) 183
BstMWI GCNNNNNNNGC 2 cut(s) 83, 131
BstNSI RCATGY 1 cut(s) 134
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 43, 46
BstV2I GAAGAC 2 cut(s) 111, 263
BstXI CCANNNNNNTGG 1 cut(s) 94
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 3 cut(s) 207, 251, 315
BtrI CACGTC 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 88, 123, 160
CfoI GCGC 3 cut(s) 20, 79, 92
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 205, 314
CseI GACGC 1 cut(s) 251
CviAII CATG 3 cut(s) 131, 139, 232
CviJI RGCY 8 cut(s) 37, 67, 86, 207, 213, 251, 259, 315
CviKI_1 RGCY 8 cut(s) 37, 67, 86, 207, 213, 251, 259, 315
DdeI CTNAG 1 cut(s) 179
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 249
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 261
Eco47III AGCGCT 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 3 cut(s) 134, 142, 235
FaiI YATR 3 cut(s) 132, 140, 233
FatI CATG 3 cut(s) 130, 138, 231
Fnu4HI GCNGC 3 cut(s) 32, 35, 135
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 3 cut(s) 32, 35, 135
GlaI GCGC 3 cut(s) 19, 78, 91
GluI GCNGC 3 cut(s) 32, 35, 135
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 2 cut(s) 80, 93
HaeIII GGCC 3 cut(s) 207, 251, 315
HapII CCGG 4 cut(s) 126, 159, 267, 312
HgaI GACGC 1 cut(s) 251
HhaI GCGC 3 cut(s) 20, 79, 92
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 3 cut(s) 134, 142, 235
Hin6I GCGC 3 cut(s) 18, 77, 90
HinP1I GCGC 3 cut(s) 18, 77, 90
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 4 cut(s) 49, 166, 298, 308
HpaII CCGG 4 cut(s) 126, 159, 267, 312
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 3 cut(s) 9, 95, 181
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 4 cut(s) 25, 245, 257, 293
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 131
HpyF3I CTNAG 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 3 cut(s) 134, 142, 235
HspAI GCGC 3 cut(s) 18, 77, 90
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 9 cut(s) 65, 80, 100, 139, 166, 172, 221, 275, 280
Lsp1109I GCAGC 2 cut(s) 43, 46
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 111
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 2 cut(s) 116, 263
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 1 cut(s) 197
MlyI GAGTC 3 cut(s) 58, 160, 292
MnlI CCTC 7 cut(s) 5, 8, 135, 188, 196, 224, 290
MreI CGCCGGCG 1 cut(s) 158
MroNI GCCGGC 1 cut(s) 158
MseI TTAA 1 cut(s) 219
MspI CCGG 4 cut(s) 126, 159, 267, 312
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 83, 131
NaeI GCCGGC 1 cut(s) 160
NdeII GATC 1 cut(s) 183
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 3 cut(s) 134, 142, 235
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 1 cut(s) 111
NspI RCATGY 1 cut(s) 134
PcsI WCGNNNNNNNCGW 2 cut(s) 49, 249
PdiI GCCGGC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 3 cut(s) 33, 36, 136
PleI GAGTC 3 cut(s) 57, 160, 292
PpsI GAGTC 3 cut(s) 57, 160, 292
Psp124BI GAGCTC 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 205, 314
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 3 cut(s) 32, 35, 135
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 2 cut(s) 205, 314
SchI GAGTC 3 cut(s) 58, 160, 292
SduI GDGCHC 1 cut(s) 261
SetI ASST 5 cut(s) 39, 69, 111, 180, 261
SgrAI CRCCGGYG 1 cut(s) 158
Sse9I AATT 1 cut(s) 197
SsiI CCGC 2 cut(s) 135, 229
SstI GAGCTC 1 cut(s) 261
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 111
TaqI TCGA 2 cut(s) 52, 201
TasI AATT 1 cut(s) 197
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 2 cut(s) 31, 34
Tsp45I GTSAC 1 cut(s) 111
XapI RAATTY 1 cut(s) 197
XceI RCATGY 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.