pycom06g21450

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0016082
Physical Location & Seq
Reverse (-)
24090374 .. 24090706
333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g21450.1

Sequence Viewer

Length: 333 bp
ATGGGTTCGAGAGGAGCAGCGACGGTGGTGTCGCAGCTGACACGAGCAAGCAGAATGAGGTCGAAATTGCAGACGGCGTTGGAAGCGAGCGTTTTGGAGATTGAAGACGTGTCGCATCAGCACGCCGGCCACGCCGCCATGAGAGAGAAGGCCAATAGCAATGGCGAAACTCACTTCAACCTCAAGGTCGTGTCCCCAAAGTTCGAGGGGCAGAGCCTGGTGAAACGACACCGCATGGTCTACGATGCCTTGGCCGACGAGCTCCAGTCCGGGCTCCACGCGCTGTCCATCGTGGCCAAGACTCCCCAAGAGATTGTCCTCAACCCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

111

Amino Acids

12.01

Weight (kDa)

9.52

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 23 - 104 5.1e-32 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55805
fragaria_vesca FvH4_5g18310
malus_domestica MD06G1238900.v1.1
prunus_persica Prupe.5G242800_v2.0.a1
pyrus_communis pycom06g21450
rosa_chinensis RchiOBHm_Chr7g0177271
rosa_laevigata RLG00000005550
rosa_multiflora Rmu_sc0005310.1_g000011
rosa_roxburghii Rroxscaffold_3G00276000
rosa_rugosa Rorug06G0404300
rosa_samantha Rh7AG004300 Rh7BG004100 Rh7CG004000 Rh7DG004000
rosa_wichuraiana Rw7G000350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 281
AciI CCGC 2 cut(s) 135, 232
AcoI YGGCCR 3 cut(s) 127, 252, 294
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 2 cut(s) 104, 178
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 2 cut(s) 37, 262
AluI AGCT 2 cut(s) 37, 262
Alw21I GWGCWC 1 cut(s) 264
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 4 cut(s) 127, 150, 252, 294
ApeKI GCWGC 2 cut(s) 17, 34
AspLEI GCGC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 232
BalI TGGCCA 1 cut(s) 296
BanII GRGCYC 2 cut(s) 264, 276
BauI CACGAG 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 29, 46
BccI CCATC 1 cut(s) 296
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 218
BcnI CCSGG 1 cut(s) 271
BisI GCNGC 3 cut(s) 18, 35, 135
BlsI GCNGC 3 cut(s) 19, 36, 136
Bme1390I CCNGG 2 cut(s) 218, 271
BmgBI CACGTC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 2 cut(s) 218, 271
BmsI GCATC 2 cut(s) 124, 235
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 249
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 249
BseMI GCAATG 1 cut(s) 166
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 29, 46
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 4 cut(s) 129, 152, 254, 296
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 2 cut(s) 126, 270
BslFI GGGAC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 4 cut(s) 129, 152, 254, 296
Bsp1286I GDGCHC 2 cut(s) 264, 276
BspACI CCGC 2 cut(s) 135, 232
BspANI GGCC 4 cut(s) 129, 152, 254, 296
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 249
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 25
BstC8I GCNNGC 4 cut(s) 49, 88, 123, 127
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 283
BstMWI GCNNNNNNNGC 3 cut(s) 83, 131, 280
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 216, 269
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 29, 46
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 4 cut(s) 129, 152, 254, 296
BtrI CACGTC 1 cut(s) 109
Cac8I GCNNGC 4 cut(s) 49, 88, 123, 127
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 125
CviAII CATG 2 cut(s) 139, 235
CviJI RGCY 8 cut(s) 37, 129, 152, 216, 254, 262, 274, 296
CviKI_1 RGCY 8 cut(s) 37, 129, 152, 216, 254, 262, 274, 296
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 3 cut(s) 127, 252, 294
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 2 cut(s) 264, 276
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 216
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 2 cut(s) 264, 276
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 2 cut(s) 142, 238
FaiI YATR 2 cut(s) 140, 236
FaqI GGGAC 1 cut(s) 178
FatI CATG 2 cut(s) 138, 234
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 3 cut(s) 18, 35, 135
FriOI GRGCYC 2 cut(s) 264, 276
Fsp4HI GCNGC 3 cut(s) 18, 35, 135
GlaI GCGC 1 cut(s) 282
GluI GCNGC 3 cut(s) 18, 35, 135
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 4 cut(s) 129, 152, 254, 296
HapII CCGG 2 cut(s) 126, 270
HhaI GCGC 1 cut(s) 283
Hin1II CATG 2 cut(s) 142, 238
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 1 cut(s) 301
HpaII CCGG 2 cut(s) 126, 270
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 241
Hpy188III TCNNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 2 cut(s) 25, 260
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 131, 280
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 2 cut(s) 142, 238
HspAI GCGC 1 cut(s) 281
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
LmnI GCTCC 3 cut(s) 14, 267, 279
LpnPI CCDG 5 cut(s) 139, 203, 230, 278, 283
Lsp1109I GCAGC 2 cut(s) 29, 46
LweI GCATC 2 cut(s) 124, 235
MaeII ACGT 1 cut(s) 108
MboII GAAGA 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 264, 276
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 5 cut(s) 5, 51, 191, 199, 329
Mox20I TGGCCA 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 2 cut(s) 126, 270
MspR9I CCNGG 2 cut(s) 218, 271
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 83, 131, 280
NaeI GCCGGC 1 cut(s) 127
NciI CCSGG 1 cut(s) 271
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 2 cut(s) 142, 238
NlaIV GGNNCC 1 cut(s) 275
PdiI GCCGGC 1 cut(s) 127
PkrI GCNGC 3 cut(s) 19, 36, 136
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 275
PstNI CAGNNNCTG 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 37
SacI GAGCTC 1 cut(s) 264
SatI GCNGC 3 cut(s) 18, 35, 135
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 2 cut(s) 218, 271
SduI GDGCHC 2 cut(s) 264, 276
SetI ASST 6 cut(s) 39, 62, 111, 183, 189, 264
SfaNI GCATC 2 cut(s) 124, 235
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 1 cut(s) 65
SsiI CCGC 2 cut(s) 135, 232
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 2 cut(s) 216, 269
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 111
TaqI TCGA 3 cut(s) 8, 62, 204
TasI AATT 1 cut(s) 65
TauI GCSGC 1 cut(s) 137
TseI GCWGC 2 cut(s) 17, 34
XmiI GTMKAC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.