FvH4_5g19970

Gibberellin 20 oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016855
Physical Location & Seq
Forward (+)
11769203 .. 11770680
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19970.t1

Sequence Viewer

Length: 1077 bp
ATGTCTTTGCAAATCGAAGAACAATATGTTGAGTCTCTCTCCTCCATGGGACACAAACTAAGTGTCAAGAACTTCATTTGGTCCAAAGAAGAATGGCCATCAATAAATCATGATGAGTTTACAGATGCGCACGACATTCCTGTCATAAGTCTCCAAGAGATTTCGGATGTGAGAAGAAACCCTAATTATGAGAAAGTCTGCGAGGACATGGTGAGTGCTTGTCACAAATGGGGGTTTTTCAAGTTAGTGGATCATGGGGTTGCTCTAGAGATTGTAAAGAGATTCAAAGGGAGTATGAATGATCTATTTGATCTTCCAATGGAGCAGAAGCTGAAGGGTGCTAGATCAACTAGTTTACCTCTGGGCTACTGTGCAACAAATCCTGATTATGGGAAGAATTTACCTTGGGCAGAGATCTTACAGTTGCTCCAGTCACCACAAATGGTGGTTGCATTTGCAACAAAGGTTTTTGGTGATCAACATCAGCCATTTAGTGATGCGATGATTGATTATCTAAATGCATTGGATAATCTGGGAATGACCATATTGGAAATGCTAGCTCATGGATTGGGTCTTCCAGACAACTTTTTCACAAAGAACTTTGAAGCAAAAGAGGCTACAATGATCAGAGTCAACAGATATCCTCCTTGTCCTCTCCCAGAAAAATGTCTTGGGCTGGGAAGCCATTCAGACCCTCATACCTTAACCATATTGTTACAAGATGATGTTGGTGGTCTTCAAGTTCATATGAATGACAACAAATGGATTGGCATCCGTCCTGTTCCAAATTCTTTCATCATCAACATTGGTGACACTCTTGAGGCTTGGACTAATGGGAGGTTGAAGAGTGTAGTGCATAGAGCAGTTGTGAACAAGGAGAAGAATAGGCTATCAGCAGCCTATTTCATGAGCCCCTCTGATTCTGCCACAATTGAGAGCCCTCCTGAGCTCATGAACCCAATAATCAACCCTAGAAAGTACGAGTCATTCACTTGGGGAGACTTCAAAAAGCAACTTTTGGTTCAAAAAAGGGTTGTTGGTAAAACGGCCCTTGCAAGATATCTAATTCCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

359

Amino Acids

40.52

Weight (kDa)

6.13

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 46 - 145 6.4e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 305 1.7e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19970
malus_domestica MD04G1049700.v1.1 MD06G1050300.v1.1
prunus_persica Prupe.5G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204791
rosa_laevigata RLG00000003433
rosa_multiflora Rmu_sc0003221.1_g000048
rosa_roxburghii Rroxscaffold_3G00252820
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216000 Rh7BG213100 Rh7CG229300 Rh7DG223600
rosa_wichuraiana Rw7G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
Acc16I TGCGCA 1 cut(s) 129
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 2 cut(s) 397, 787
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 1 cut(s) 980
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 7 cut(s) 241, 286, 605, 740, 844, 1006, 1025
AhlI ACTAGT 1 cut(s) 350
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
AluBI AGCT 3 cut(s) 331, 560, 949
AluI AGCT 3 cut(s) 331, 560, 949
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 3 cut(s) 39, 155, 993
AlwI GGATC 1 cut(s) 258
AlwNI CAGNNNCTG 1 cut(s) 331
AoxI GGCC 2 cut(s) 95, 1047
ApeKI GCWGC 1 cut(s) 896
ApoI RAATTY 2 cut(s) 397, 787
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
Asp700I GAANNNNTTC 1 cut(s) 685
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 81, 1048
AsuHPI GGTGA 4 cut(s) 223, 426, 485, 821
AsuNHI GCTAGC 1 cut(s) 556
AvaII GGWCC 1 cut(s) 81
BalI TGGCCA 1 cut(s) 97
BanII GRGCYC 3 cut(s) 914, 941, 951
BbsI GAAGAC 2 cut(s) 566, 728
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 1 cut(s) 908
BccI CCATC 1 cut(s) 106
BceAI ACGGC 1 cut(s) 1062
BclI TGATCA 2 cut(s) 475, 624
BcoDI GTCTC 3 cut(s) 39, 155, 993
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 5 cut(s) 266, 342, 351, 557, 972
BglII AGATCT 1 cut(s) 414
BisI GCNGC 1 cut(s) 897
BlsI GCNGC 1 cut(s) 898
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 81, 1048
BmsI GCATC 3 cut(s) 115, 487, 780
BmtI GCTAGC 1 cut(s) 560
BpiI GAAGAC 2 cut(s) 566, 728
BplI GAGNNNNNCTC 2 cut(s) 23, 55
BpmI CTGGAG 1 cut(s) 413
Bpu10I CCTNAGC 1 cut(s) 945
BpuEI CTTGAG 1 cut(s) 839
BsaBI GATNNNNATC 2 cut(s) 480, 770
BsaJI CCNNGG 2 cut(s) 45, 404
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 430
Bse8I GATNNNNATC 2 cut(s) 480, 770
BseDI CCNNGG 2 cut(s) 45, 404
BseGI GGATG 2 cut(s) 172, 771
BseJI GATNNNNATC 2 cut(s) 480, 770
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 1 cut(s) 936
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 908
BseYI CCCAGC 1 cut(s) 676
BshFI GGCC 2 cut(s) 97, 1049
BsiHKAI GWGCWC 1 cut(s) 951
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 3 cut(s) 39, 155, 993
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 2 cut(s) 97, 1049
Bsp1286I GDGCHC 3 cut(s) 914, 941, 951
Bsp143I GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 2 cut(s) 97, 1049
BspCNI CTCAG 1 cut(s) 937
BspHI TCATGA 3 cut(s) 109, 906, 951
BspOI GCTAGC 1 cut(s) 560
BspPI GGATC 1 cut(s) 258
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 2 cut(s) 45, 404
BssMI GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
BssT1I CCWWGG 2 cut(s) 45, 404
Bst4CI ACNGT 2 cut(s) 371, 423
Bst6I CTCTTC 1 cut(s) 839
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 59, 945
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 172, 771
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 7 cut(s) 253, 304, 313, 347, 417, 478, 627
BstMAI GTCTC 3 cut(s) 39, 155, 993
BstMBI GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstV1I GCAGC 1 cut(s) 908
BstV2I GAAGAC 2 cut(s) 566, 728
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 2 cut(s) 97, 1049
BtgI CCRYGG 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 2 cut(s) 172, 771
Cac8I GCNNGC 1 cut(s) 558
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 3 cut(s) 109, 906, 951
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 81, 1048
Csp6I GTAC 1 cut(s) 979
CviAII CATG 7 cut(s) 46, 110, 208, 254, 563, 907, 952
CviQI GTAC 1 cut(s) 979
DdeI CTNAG 2 cut(s) 59, 945
DpnI GATC 7 cut(s) 252, 303, 312, 346, 416, 477, 626
DpnII GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 839
EarI CTCTTC 1 cut(s) 839
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 2 cut(s) 45, 404
Eco24I GRGCYC 3 cut(s) 914, 941, 951
Eco32I GATATC 2 cut(s) 641, 1061
Eco47I GGWCC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 1 cut(s) 353
EcoICRI GAGCTC 1 cut(s) 949
EcoRV GATATC 2 cut(s) 641, 1061
EcoT14I CCWWGG 2 cut(s) 45, 404
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 3 cut(s) 914, 941, 951
ErhI CCWWGG 2 cut(s) 45, 404
FaeI CATG 7 cut(s) 49, 113, 211, 257, 566, 910, 955
FaqI GGGAC 1 cut(s) 63
FatI CATG 7 cut(s) 45, 109, 207, 253, 562, 906, 951
FauNDI CATATG 1 cut(s) 747
FbaI TGATCA 2 cut(s) 475, 624
Fnu4HI GCNGC 1 cut(s) 897
FokI GGATG 2 cut(s) 179, 758
FriOI GRGCYC 3 cut(s) 914, 941, 951
Fsp4HI GCNGC 1 cut(s) 897
FspAI RTGCGCAY 1 cut(s) 129
FspBI CTAG 5 cut(s) 266, 342, 351, 557, 972
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 1 cut(s) 129
GluI GCNGC 1 cut(s) 897
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 413
HaeIII GGCC 2 cut(s) 97, 1049
HhaI GCGC 1 cut(s) 130
Hin1II CATG 7 cut(s) 49, 113, 211, 257, 566, 910, 955
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 5 cut(s) 32, 282, 630, 920, 983
HphI GGTGA 4 cut(s) 223, 426, 485, 821
Hpy166II GTNNAC 4 cut(s) 120, 356, 634, 871
Hpy188I TCNGA 4 cut(s) 166, 629, 691, 919
Hpy188III TCNNGA 9 cut(s) 67, 110, 266, 383, 578, 818, 907, 944, 952
Hpy8I GTNNAC 4 cut(s) 120, 356, 634, 871
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 2 cut(s) 371, 423
HpyCH4V TGCA 7 cut(s) 10, 374, 452, 458, 521, 856, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 2 cut(s) 59, 945
Hsp92II CATG 7 cut(s) 49, 113, 211, 257, 566, 910, 955
HspAI GCGC 1 cut(s) 128
Ksp22I TGATCA 2 cut(s) 475, 624
Kzo9I GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
LmnI GCTCC 2 cut(s) 322, 432
Lsp1109I GCAGC 1 cut(s) 908
LweI GCATC 3 cut(s) 115, 487, 780
MaeI CTAG 5 cut(s) 266, 342, 351, 557, 972
MaeIII GTNAC 4 cut(s) 221, 432, 714, 809
MalI GATC 7 cut(s) 252, 303, 312, 346, 416, 477, 626
MboI GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
MboII GAAGA 9 cut(s) 29, 101, 186, 305, 406, 566, 728, 856, 892
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 3 cut(s) 914, 941, 951
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 5 cut(s) 184, 397, 787, 930, 1065
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 3 cut(s) 41, 639, 992
Mox20I TGGCCA 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 685
MscI TGGCCA 1 cut(s) 97
MseI TTAA 1 cut(s) 704
MslI CAYNNNNRTG 1 cut(s) 750
Msp20I TGGCCA 1 cut(s) 97
MunI CAATTG 1 cut(s) 930
MwoI GCNNNNNNNGC 1 cut(s) 614
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 747
NdeII GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
NheI GCTAGC 1 cut(s) 556
NlaIII CATG 7 cut(s) 49, 113, 211, 257, 566, 910, 955
NmuCI GTSAC 3 cut(s) 221, 432, 809
NsbI TGCGCA 1 cut(s) 129
NsiI ATGCAT 1 cut(s) 523
PagI TCATGA 3 cut(s) 109, 906, 951
PdmI GAANNNNTTC 1 cut(s) 685
PfeI GAWTC 2 cut(s) 282, 920
PkrI GCNGC 1 cut(s) 898
PleI GAGTC 3 cut(s) 40, 638, 991
PpsI GAGTC 3 cut(s) 40, 638, 991
Psp124BI GAGCTC 1 cut(s) 951
PspFI CCCAGC 1 cut(s) 676
PspPI GGNCC 2 cut(s) 81, 1048
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
RseI CAYNNNNRTG 1 cut(s) 750
SacI GAGCTC 1 cut(s) 951
SaqAI TTAA 1 cut(s) 704
SatI GCNGC 1 cut(s) 897
Sau3AI GATC 7 cut(s) 250, 301, 310, 344, 414, 475, 624
Sau96I GGNCC 2 cut(s) 81, 1048
SchI GAGTC 3 cut(s) 41, 639, 992
SduI GDGCHC 3 cut(s) 914, 941, 951
SetI ASST 8 cut(s) 333, 361, 406, 468, 562, 704, 842, 951
SfaNI GCATC 3 cut(s) 115, 487, 780
SinI GGWCC 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 750
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 5 cut(s) 184, 397, 787, 930, 1065
SspMI CTAG 5 cut(s) 266, 342, 351, 557, 972
SstI GAGCTC 1 cut(s) 951
StyI CCWWGG 2 cut(s) 45, 404
TaaI ACNGT 2 cut(s) 371, 423
TaqI TCGA 1 cut(s) 15
TasI AATT 5 cut(s) 184, 397, 787, 930, 1065
TfiI GAWTC 2 cut(s) 282, 920
Tru1I TTAA 1 cut(s) 704
Tru9I TTAA 1 cut(s) 704
TseFI GTSAC 3 cut(s) 221, 432, 809
TseI GCWGC 1 cut(s) 896
Tsp45I GTSAC 3 cut(s) 221, 432, 809
TspDTI ATGAA 7 cut(s) 64, 311, 734, 764, 784, 895, 968
TspGWI ACGGA 1 cut(s) 764
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 2 cut(s) 397, 787
XbaI TCTAGA 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 685
XspI CTAG 5 cut(s) 266, 342, 351, 557, 972
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.