Rh7DG223600

Gibberellin 20 oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0016855
Physical Location & Seq
Forward (+)
21792042 .. 21800457
8416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG223600.1

Sequence Viewer

Length: 1077 bp
ATGGCTTTGCAAATCGAAGAACAATACGTCGAGTCACTCTCCTCCATGGGACACAAACTAAGTGTCAAGAACTTCATTTGGTCCAAGGAAGAATGGCCAGCAATAAATCATGATGAGTTTACAGATTCACATGACATTCCTGCAATAAGTCTCCGAGAGATTTCAGATGGGAGAAGAAACCCTAATTATGAGAAGGTCTGCAAGGTCATGGTGAATGCTTGTGAGAAATGGGGGTTCTTCAAGTTAGTGGATCATGGGGTTGCTTTGGAGATCATAGAGAATTTCAAAGGGAGTATGAATGCGCTTTTTGATCTTCCAATGGAGCAGAAGCTGAAGGGTGTTAGATCAGCTAGTTTACCATTAGGTTACTGTGCATCAAATCCTGATTATGGGAAGAATTTGCCCTGGGCAGAGATCTTACAGTTGCTCCAGTCACCACAAATGGTGGTTGGATTTGCAACAAAGGTTTTTGGTGATCAACATCAGCCGTTCAGTGATGCAATGATTGGTTACTTGAATGCATTGGATAATCTGGGAATGACTATTTTGGAGATGCTAGCTCATGGATTGGGTCTTCCAGACAGCTTCTTCACAAAGAACTTTGAAGCAAAAGAGGCTACAATGATCAGAGTCAACAGATATCCTCCTTGTCCTCTTCCAGAAAAATGTCTTGGGCTGGGAAGCCATTCAGACCCTCATACCTTAACCATATTGTTACAAGATGATGTTGGTGGGCTTCAAGTTCTTATGAATGACAACAAATGGATTGGCATCCGTCCTGTTCAGAATTCTTTCATTATCAACATTGGTGACACCCTTGAGGCTTGGACTAATGGGAGGCTGAAGAGTGTTGTGCATAGAGCAGTTGTCAACAAGGAGAAGAACAGGCTATCGGCAGCTTATTTCATGAGCCCCTCTGATTCTGCCACAATTGAGTGCCCTCCCGAGCTCATGGTCCCAATAATCAACCCTAGAAAGTATGACTCATTCACTTGGGGAGACTTCAAGAAGCAACTTTTGGTTCAAAAAAGGGTTGTTGGTAAAACGGCCCTCGAAAGATATCTAATTTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

358

Amino Acids

40.32

Weight (kDa)

6.16

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 46 - 145 2.1e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 305 2.5e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19970
malus_domestica MD04G1049700.v1.1 MD06G1050300.v1.1
prunus_persica Prupe.5G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204791
rosa_laevigata RLG00000003433
rosa_multiflora Rmu_sc0003221.1_g000048
rosa_roxburghii Rroxscaffold_3G00252820
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216000 Rh7BG213100 Rh7CG229300 Rh7DG223600
rosa_wichuraiana Rw7G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 3 cut(s) 280, 397, 787
AcuI CTGAAG 2 cut(s) 353, 863
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 8 cut(s) 241, 286, 517, 605, 740, 1006, 1025, 1071
AjnI CCWGG 1 cut(s) 404
AjuI GAANNNNNNNTTGG 4 cut(s) 654, 686, 1001, 1033
AluBI AGCT 6 cut(s) 331, 350, 560, 585, 899, 949
AluI AGCT 6 cut(s) 331, 350, 560, 585, 899, 949
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 155, 993
AlwI GGATC 1 cut(s) 258
AlwNI CAGNNNCTG 1 cut(s) 331
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 2 cut(s) 95, 1047
ApeKI GCWGC 1 cut(s) 896
ApoI RAATTY 3 cut(s) 280, 397, 787
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
Asp700I GAANNNNTTC 2 cut(s) 685, 791
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 3 cut(s) 81, 955, 1048
AsuHPI GGTGA 4 cut(s) 223, 426, 485, 821
AsuNHI GCTAGC 1 cut(s) 556
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 2 cut(s) 81, 955
BaeGI GKGCMC 1 cut(s) 941
BalI TGGCCA 1 cut(s) 97
BanII GRGCYC 2 cut(s) 914, 951
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 1 cut(s) 908
BccI CCATC 1 cut(s) 161
BceAI ACGGC 2 cut(s) 472, 1062
BciT130I CCWGG 1 cut(s) 406
BclI TGATCA 2 cut(s) 475, 624
BcoDI GTCTC 2 cut(s) 155, 993
BfaI CTAG 3 cut(s) 351, 557, 972
BglII AGATCT 1 cut(s) 414
BisI GCNGC 1 cut(s) 897
BlsI GCNGC 1 cut(s) 898
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 2 cut(s) 81, 955
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 3 cut(s) 81, 955, 1048
BmiI GGNNCC 1 cut(s) 957
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 4 cut(s) 383, 487, 543, 780
BmtI GCTAGC 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 566
BplI GAGNNNNNCTC 2 cut(s) 23, 55
BpmI CTGGAG 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 839
BsaBI GATNNNNATC 2 cut(s) 480, 770
BsaJI CCNNGG 4 cut(s) 45, 84, 404, 405
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 430
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 2 cut(s) 480, 770
BseBI CCWGG 1 cut(s) 406
BseDI CCNNGG 4 cut(s) 45, 84, 404, 405
BseGI GGATG 1 cut(s) 771
BseJI GATNNNNATC 2 cut(s) 480, 770
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 31
BseSI GKGCMC 1 cut(s) 941
BseXI GCAGC 1 cut(s) 908
BseYI CCCAGC 1 cut(s) 676
BshFI GGCC 2 cut(s) 97, 1049
BsiHKAI GWGCWC 1 cut(s) 951
BsiHKCI CYCGRG 1 cut(s) 944
BslFI GGGAC 2 cut(s) 63, 941
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 155, 993
BsmFI GGGAC 2 cut(s) 63, 941
BsmI GAATGC 3 cut(s) 220, 304, 523
BsnI GGCC 2 cut(s) 97, 1049
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 3 cut(s) 914, 941, 951
Bsp143I GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 2 cut(s) 97, 1049
BspHI TCATGA 2 cut(s) 109, 906
BspLI GGNNCC 1 cut(s) 957
BspOI GCTAGC 1 cut(s) 560
BspPI GGATC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 4 cut(s) 45, 84, 404, 405
BssMI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
BssT1I CCWWGG 2 cut(s) 45, 84
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 2 cut(s) 371, 423
Bst6I CTCTTC 2 cut(s) 660, 839
BstC8I GCNNGC 2 cut(s) 99, 558
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 771
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 7 cut(s) 253, 273, 313, 347, 417, 478, 627
BstMAI GTCTC 2 cut(s) 155, 993
BstMBI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstNI CCWGG 1 cut(s) 406
BstSCI CCNGG 1 cut(s) 404
BstSLI GKGCMC 1 cut(s) 941
BstV1I GCAGC 1 cut(s) 908
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 2 cut(s) 97, 1049
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 99, 558
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 2 cut(s) 109, 906
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 3 cut(s) 81, 955, 1048
CviAII CATG 8 cut(s) 46, 110, 131, 208, 254, 563, 907, 952
DdeI CTNAG 1 cut(s) 59
DpnI GATC 7 cut(s) 252, 272, 312, 346, 416, 477, 626
DpnII GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 660, 839
EarI CTCTTC 2 cut(s) 660, 839
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 2 cut(s) 45, 84
Eco24I GRGCYC 2 cut(s) 914, 951
Eco32I GATATC 2 cut(s) 641, 1061
Eco47I GGWCC 2 cut(s) 81, 955
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 2 cut(s) 353, 863
Eco88I CYCGRG 1 cut(s) 944
EcoICRI GAGCTC 1 cut(s) 949
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 1 cut(s) 404
EcoRV GATATC 2 cut(s) 641, 1061
EcoT14I CCWWGG 2 cut(s) 45, 84
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 914, 951
ErhI CCWWGG 2 cut(s) 45, 84
FaeI CATG 8 cut(s) 49, 113, 134, 211, 257, 566, 910, 955
FaqI GGGAC 2 cut(s) 63, 941
FatI CATG 8 cut(s) 45, 109, 130, 207, 253, 562, 906, 951
FbaI TGATCA 2 cut(s) 475, 624
Fnu4HI GCNGC 1 cut(s) 897
FokI GGATG 1 cut(s) 758
FriOI GRGCYC 2 cut(s) 914, 951
Fsp4HI GCNGC 1 cut(s) 897
FspBI CTAG 3 cut(s) 351, 557, 972
GlaI GCGC 1 cut(s) 303
GluI GCNGC 1 cut(s) 897
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 413
HaeIII GGCC 2 cut(s) 97, 1049
HhaI GCGC 1 cut(s) 304
Hin1II CATG 8 cut(s) 49, 113, 134, 211, 257, 566, 910, 955
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 2 cut(s) 634, 871
HindII GTYRAC 2 cut(s) 634, 871
HinfI GANTC 5 cut(s) 32, 125, 630, 920, 983
HphI GGTGA 4 cut(s) 223, 426, 485, 821
Hpy166II GTNNAC 4 cut(s) 120, 356, 634, 871
Hpy188I TCNGA 6 cut(s) 155, 166, 629, 691, 786, 919
Hpy188III TCNNGA 8 cut(s) 67, 110, 383, 578, 659, 907, 944, 1006
Hpy8I GTNNAC 4 cut(s) 120, 356, 634, 871
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 187, 328
HpyCH4III ACNGT 2 cut(s) 371, 423
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 8 cut(s) 10, 143, 201, 374, 458, 500, 521, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 8 cut(s) 49, 113, 134, 211, 257, 566, 910, 955
HspAI GCGC 1 cut(s) 302
Ksp22I TGATCA 2 cut(s) 475, 624
Kzo9I GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
LmnI GCTCC 2 cut(s) 322, 432
Lsp1109I GCAGC 1 cut(s) 908
LweI GCATC 4 cut(s) 383, 487, 543, 780
MaeI CTAG 3 cut(s) 351, 557, 972
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 6 cut(s) 33, 365, 432, 509, 714, 809
MalI GATC 7 cut(s) 252, 272, 312, 346, 416, 477, 626
MboI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 3 cut(s) 914, 941, 951
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 6 cut(s) 184, 280, 397, 787, 930, 1065
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 3 cut(s) 41, 639, 977
MmeI TCCRAC 1 cut(s) 430
Mox20I TGGCCA 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 2 cut(s) 685, 791
MscI TGGCCA 1 cut(s) 97
MseI TTAA 1 cut(s) 704
Msp20I TGGCCA 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 406
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 3 cut(s) 220, 304, 523
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 614
NcoI CCATGG 1 cut(s) 45
NdeII GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
NheI GCTAGC 1 cut(s) 556
NlaIII CATG 8 cut(s) 49, 113, 134, 211, 257, 566, 910, 955
NlaIV GGNNCC 1 cut(s) 957
NmuCI GTSAC 3 cut(s) 33, 432, 809
NsiI ATGCAT 1 cut(s) 523
PagI TCATGA 2 cut(s) 109, 906
PasI CCCWGGG 1 cut(s) 405
PctI GAATGC 3 cut(s) 220, 304, 523
PdmI GAANNNNTTC 2 cut(s) 685, 791
PfeI GAWTC 2 cut(s) 125, 920
PkrI GCNGC 1 cut(s) 898
PleI GAGTC 3 cut(s) 40, 638, 977
PpsI GAGTC 3 cut(s) 40, 638, 977
Psp124BI GAGCTC 1 cut(s) 951
Psp6I CCWGG 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 957
PspPI GGNCC 3 cut(s) 81, 955, 1048
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 414
SacI GAGCTC 1 cut(s) 951
SaqAI TTAA 1 cut(s) 704
SatI GCNGC 1 cut(s) 897
Sau3AI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
Sau96I GGNCC 3 cut(s) 81, 955, 1048
SchI GAGTC 3 cut(s) 41, 639, 977
ScrFI CCNGG 1 cut(s) 406
SduI GDGCHC 3 cut(s) 914, 941, 951
SfaNI GCATC 4 cut(s) 383, 487, 543, 780
SinI GGWCC 2 cut(s) 81, 955
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
Sse9I AATT 6 cut(s) 184, 280, 397, 787, 930, 1065
SspMI CTAG 3 cut(s) 351, 557, 972
SstI GAGCTC 1 cut(s) 951
StyD4I CCNGG 1 cut(s) 404
StyI CCWWGG 2 cut(s) 45, 84
TaaI ACNGT 2 cut(s) 371, 423
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 15, 30, 1053
TasI AATT 6 cut(s) 184, 280, 397, 787, 930, 1065
TfiI GAWTC 2 cut(s) 125, 920
Tru1I TTAA 1 cut(s) 704
Tru9I TTAA 1 cut(s) 704
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 3 cut(s) 33, 432, 809
TseI GCWGC 1 cut(s) 896
Tsp45I GTSAC 3 cut(s) 33, 432, 809
TspDTI ATGAA 5 cut(s) 64, 311, 764, 784, 895
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 2 cut(s) 81, 955
XapI RAATTY 3 cut(s) 280, 397, 787
XmnI GAANNNNTTC 2 cut(s) 685, 791
XspI CTAG 3 cut(s) 351, 557, 972
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.