MD06G1050300.v1.1

Gibberellin 20 oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016855
Physical Location & Seq
Reverse (-)
6911461 .. 6912421
961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1050300.v1.1.491

Sequence Viewer

Length: 615 bp
ATGTTTGGAATATGTATCAGCAATGCAATGATTCGTTACTTGAATGCACTGGACAATTTGGGAATGACAATTTTGGAGATGTTAGCTCATGGACTGGGCCTTCCAGACAACTTTTTCACAAAGAACTTTGAGGAAAAAGAGGCTACTATGATCAGGGTCAACAGATATCCTCCTTGTCCCCTCCCTGAAAAATGTCTTGGGCTTGGGAGCCATTCAGACCCACATACCTTAACCATATTGTTACAAGATGATGTTGGTGGCCTGCAAGTTCTTACAAGTGACAACAAATGGATTGGTATCCGTCCTGTTCAAAATTCTTTCATCATCAACATCGGTGACACCCTTGAAGCTTGGACTAATGGGAAGCTGAGGAGTGTAGTGCATAGAGCAGTGCTTAACAAGGAGAAGAATAGGCTATCAGCAGCATATTTCATGAGCCCCCGTAATTCTGCCCTCATTGACTGCCCTCTTGTGCTCATGGATGCAAAAACCAACCCTAGAAAATATGTGCCCTTTACTTGGGGTAATTTCCGAAAGCAACTTTTGGTTCAAAAGAGGGTTGTTGGTAACTGCACTAGAAAGATACCTAATATCCAAATGAAGATTATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

205

Amino Acids

23.04

Weight (kDa)

9.51

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 50 - 147 1.7e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19970
malus_domestica MD04G1049700.v1.1 MD06G1050300.v1.1
prunus_persica Prupe.5G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204791
rosa_laevigata RLG00000003433
rosa_multiflora Rmu_sc0003221.1_g000048
rosa_roxburghii Rroxscaffold_3G00252820
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216000 Rh7BG213100 Rh7CG229300 Rh7DG223600
rosa_wichuraiana Rw7G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 313
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 4 cut(s) 43, 311, 347, 551
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 3 cut(s) 86, 350, 367
AluI AGCT 3 cut(s) 86, 350, 367
Alw21I GWGCWC 1 cut(s) 477
AoxI GGCC 2 cut(s) 97, 259
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 313
AspS9I GGNCC 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 513
BanII GRGCYC 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 477
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 434
BciVI GTATCC 1 cut(s) 308
BclI TGATCA 1 cut(s) 150
BfaI CTAG 2 cut(s) 498, 576
BfuI GTATCC 1 cut(s) 308
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 209
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 1 cut(s) 472
BmuI ACTGGG 1 cut(s) 104
Bpu10I CCTNAGC 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 54, 99
Bse3DI GCAATG 2 cut(s) 28, 33
Bse8I GATNNNNATC 1 cut(s) 296
BseGI GGATG 1 cut(s) 487
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMI GCAATG 2 cut(s) 28, 33
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 54, 99
BseRI GAGGAG 1 cut(s) 385
BseSI GKGCMC 1 cut(s) 513
BseXI GCAGC 1 cut(s) 434
BsgI GTGCAG 1 cut(s) 556
BshFI GGCC 2 cut(s) 99, 261
BsiHKAI GWGCWC 1 cut(s) 477
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 519
BsmFI GGGAC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 2 cut(s) 99, 261
Bsp1286I GDGCHC 3 cut(s) 440, 477, 513
Bsp143I GATC 1 cut(s) 150
BspANI GGCC 2 cut(s) 99, 261
BspCNI CTCAG 1 cut(s) 360
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 28, 33
BsrI ACTGG 2 cut(s) 54, 99
BssMI GATC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 368
BstF5I GGATG 1 cut(s) 487
BstKTI GATC 1 cut(s) 153
BstMBI GATC 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 513
BstV1I GCAGC 1 cut(s) 434
BsuI GTATCC 1 cut(s) 308
BsuRI GGCC 2 cut(s) 99, 261
BtsCI GGATG 1 cut(s) 487
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 47, 396
Cac8I GCNNGC 1 cut(s) 263
CciI TCATGA 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 97
CviAII CATG 3 cut(s) 89, 433, 478
DdeI CTNAG 1 cut(s) 368
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 440
Eco32I GATATC 1 cut(s) 167
EcoRV GATATC 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 440
FaeI CATG 3 cut(s) 92, 436, 481
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 88, 432, 477
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 1 cut(s) 494
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 2 cut(s) 498, 576
GluI GCNGC 1 cut(s) 423
HaeIII GGCC 2 cut(s) 99, 261
Hin1II CATG 3 cut(s) 92, 436, 481
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 1 cut(s) 31
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 217, 533
Hpy188III TCNNGA 2 cut(s) 104, 433
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 110
HpyCH4V TGCA 6 cut(s) 26, 47, 265, 382, 485, 573
HpyF3I CTNAG 1 cut(s) 368
Hsp92II CATG 3 cut(s) 92, 436, 481
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 207
LpnPI CCDG 7 cut(s) 35, 80, 117, 139, 198, 275, 318
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 1 cut(s) 472
MaeI CTAG 2 cut(s) 498, 576
MaeIII GTNAC 5 cut(s) 35, 240, 278, 335, 566
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MboII GAAGA 2 cut(s) 418, 613
MhlI GDGCHC 3 cut(s) 440, 477, 513
MluCI AATT 5 cut(s) 55, 69, 313, 445, 526
MnlI CCTC 8 cut(s) 124, 133, 180, 191, 363, 464, 477, 549
MseI TTAA 2 cut(s) 230, 396
Mva1269I GAATGC 1 cut(s) 49
NdeII GATC 1 cut(s) 150
NlaIII CATG 3 cut(s) 92, 436, 481
NlaIV GGNNCC 1 cut(s) 209
NmuCI GTSAC 2 cut(s) 278, 335
PagI TCATGA 1 cut(s) 432
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 230, 396
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 1 cut(s) 97
SduI GDGCHC 3 cut(s) 440, 477, 513
SetI ASST 5 cut(s) 88, 230, 352, 369, 589
SfaNI GCATC 1 cut(s) 472
Sse9I AATT 5 cut(s) 55, 69, 313, 445, 526
SspMI CTAG 2 cut(s) 498, 576
TasI AATT 5 cut(s) 55, 69, 313, 445, 526
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 2 cut(s) 230, 396
Tru9I TTAA 2 cut(s) 230, 396
TscAI CASTG 2 cut(s) 54, 396
TseFI GTSAC 2 cut(s) 278, 335
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 2 cut(s) 278, 335
TspDTI ATGAA 3 cut(s) 310, 421, 614
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 2 cut(s) 54, 396
XapI RAATTY 1 cut(s) 313
XspI CTAG 2 cut(s) 498, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.