Prupe.5G060600_v2.0.a1

Gibberellin 20 oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016855
Physical Location & Seq
Reverse (-)
6525308 .. 6527252
1945 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G060600.1

Sequence Viewer

Length: 1077 bp
ATGGCTCTACAAATTGAAGACCAATATGTTGAGTCACTTTCCTCCATGGGGCACAAGCTAAGTGTCAAGAATTTCATCTGGTCCACAGAAGAATGGCCAGAAATTAACCATGATGACTTTGCAGATGCAGATGACATCCCTGTCATAAGTCTCCAAGGGGTTTTGGATGGGAGGAAAAACCCTGACTATGACAAGGTTTGCCAAGTCATGGTGAGGGCTTGTGAGAAGTGGGGGTTCTTCAAGCTAGTGGATCATGGGGTTGCTCTGGAGACCATAGAGAGTTTCATAGGGAGCTTAAATGGGCTTTTTGATCTTCCAATGGAGCAGAAGTTGAAGGGTGTTAGATCAGCTAGCTTACCACTGGGTTACTATGCCACAAATCCTGATTATGGAAAAAATTTGCCTTGGGCAGAGATCTTGCAATTGCTCCAGTCCCCACAACAGGTAGTTGGATTTGCTACAAAGGTTTTTGGTGATCAACATCAGCCATTTAGCAATGCCATGATTGGCTACTTGAATGCATTAGACAATTTAGGAATGACCATTTTGGAGATGTTAGCTCATGGACTGGGCCTTCCAGATGAGTTTTTTAAGAAGAACTTTGAAGAGAAAGAGGCTACCATGATCAGGGTCAACAGATATCCACCTTGTCCTCTCCCAGAAAAATGTCTTGGGCTTGGGAGCCATTCAGACCCCCATACCTTAACCATATTGCTGCAAGATGATGTTGGTGGGCTTCAAGTTCGGATGAGCGACAAGAAATGGATTGGTATCCGCCCTGTTCAAAATTCTTTCATCATCAATATTGGTGACACCCTTGAAGCTTGGACTAATGGGAGGCTGAGGAGTGTAGTGCATAGAGCAGTGGTTAACAAGGAGAAGAATAGGCTATCAGCAGCATATTTCATGAGCCCTAGTAATTCTGCCCTCATTGAGTGCCCTCCTGAGCTCATGGACCCAAAAACCAACCCTAAAAAGTATCAGCCCTTCACTTGGGGAGGTTTCAAAAAGGAACTTCTGGTACAAAAGAGGGTTGTTGGTAAAACTGCTCTAGAAAGATATCTAATTTCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

359

Amino Acids

40.34

Weight (kDa)

6.1

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19970
malus_domestica MD04G1049700.v1.1 MD06G1050300.v1.1
prunus_persica Prupe.5G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204791
rosa_laevigata RLG00000003433
rosa_multiflora Rmu_sc0003221.1_g000048
rosa_roxburghii Rroxscaffold_3G00252820
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216000 Rh7BG213100 Rh7CG229300 Rh7DG223600
rosa_wichuraiana Rw7G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB7I CCANNNNNTGG 1 cut(s) 208
AciI CCGC 1 cut(s) 775
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 3 cut(s) 70, 397, 787
AfaI GTAC 1 cut(s) 1023
AfiI CCNNNNNNNGG 6 cut(s) 48, 208, 389, 442, 627, 993
AgsI TTSAA 9 cut(s) 17, 241, 334, 517, 605, 740, 785, 821, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
AluBI AGCT 8 cut(s) 58, 244, 294, 350, 354, 560, 824, 949
AluI AGCT 8 cut(s) 58, 244, 294, 350, 354, 560, 824, 949
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 155, 263
AlwI GGATC 1 cut(s) 258
AoxI GGCC 2 cut(s) 95, 571
ApeKI GCWGC 2 cut(s) 715, 896
ApoI RAATTY 3 cut(s) 70, 397, 787
AspS9I GGNCC 3 cut(s) 81, 571, 955
AsuHPI GGTGA 3 cut(s) 223, 485, 821
AsuNHI GCTAGC 1 cut(s) 350
AvaII GGWCC 2 cut(s) 81, 955
BaeGI GKGCMC 2 cut(s) 54, 941
BalI TGGCCA 1 cut(s) 97
BanII GRGCYC 2 cut(s) 914, 951
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 951
BbvCI CCTCAGC 1 cut(s) 842
BbvI GCAGC 2 cut(s) 702, 908
BccI CCATC 1 cut(s) 161
BciVI GTATCC 1 cut(s) 782
BclI TGATCA 2 cut(s) 475, 624
BcoDI GTCTC 2 cut(s) 155, 263
BfaI CTAG 4 cut(s) 245, 351, 915, 1052
BfuI GTATCC 1 cut(s) 782
BglII AGATCT 1 cut(s) 414
BisI GCNGC 2 cut(s) 716, 897
BlsI GCNGC 2 cut(s) 717, 898
Bme18I GGWCC 2 cut(s) 81, 955
BmgT120I GGNCC 3 cut(s) 81, 571, 955
BmiI GGNNCC 2 cut(s) 683, 957
BmrI ACTGGG 2 cut(s) 371, 578
BmsI GCATC 1 cut(s) 115
BmtI GCTAGC 1 cut(s) 354
BmuI ACTGGG 2 cut(s) 371, 578
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 2 cut(s) 287, 413
Bpu10I CCTNAGC 2 cut(s) 842, 945
BsaBI GATNNNNATC 2 cut(s) 480, 770
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 45, 154, 404
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 208, 389, 442, 627, 993
Bse1I ACTGG 3 cut(s) 366, 430, 573
Bse3DI GCAATG 1 cut(s) 502
Bse8I GATNNNNATC 2 cut(s) 480, 770
BseDI CCNNGG 3 cut(s) 45, 154, 404
BseGI GGATG 3 cut(s) 135, 172, 753
BseJI GATNNNNATC 2 cut(s) 480, 770
BseLI CCNNNNNNNGG 6 cut(s) 48, 208, 389, 442, 627, 993
BseMI GCAATG 1 cut(s) 502
BseMII CTCAG 2 cut(s) 833, 936
BseNI ACTGG 3 cut(s) 366, 430, 573
BseRI GAGGAG 1 cut(s) 859
BseSI GKGCMC 2 cut(s) 54, 941
BseXI GCAGC 2 cut(s) 702, 908
BshFI GGCC 2 cut(s) 97, 573
BsiHKAI GWGCWC 1 cut(s) 951
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 6 cut(s) 48, 208, 389, 442, 627, 993
BsmAI GTCTC 2 cut(s) 155, 263
BsmFI GGGAC 1 cut(s) 418
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 2 cut(s) 97, 573
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 4 cut(s) 54, 914, 941, 951
Bsp143I GATC 6 cut(s) 250, 310, 344, 414, 475, 624
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 775
BspANI GGCC 2 cut(s) 97, 573
BspCNI CTCAG 2 cut(s) 834, 937
BspHI TCATGA 1 cut(s) 906
BspLI GGNNCC 2 cut(s) 683, 957
BspOI GCTAGC 1 cut(s) 354
BspPI GGATC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 502
BsrI ACTGG 3 cut(s) 366, 430, 573
BssECI CCNNGG 3 cut(s) 45, 154, 404
BssMI GATC 6 cut(s) 250, 310, 344, 414, 475, 624
BssT1I CCWWGG 3 cut(s) 45, 154, 404
Bst6I CTCTTC 1 cut(s) 600
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 3 cut(s) 59, 842, 945
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 135, 172, 753
BstKTI GATC 6 cut(s) 253, 313, 347, 417, 478, 627
BstMAI GTCTC 2 cut(s) 155, 263
BstMBI GATC 6 cut(s) 250, 310, 344, 414, 475, 624
BstSLI GKGCMC 2 cut(s) 54, 941
BstV1I GCAGC 2 cut(s) 702, 908
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuI GTATCC 1 cut(s) 782
BsuRI GGCC 2 cut(s) 97, 573
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 3 cut(s) 135, 172, 753
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 2 cut(s) 359, 870
Cac8I GCNNGC 1 cut(s) 352
CciI TCATGA 1 cut(s) 906
Cfr13I GGNCC 3 cut(s) 81, 571, 955
Csp6I GTAC 1 cut(s) 1022
CviAII CATG 9 cut(s) 46, 110, 208, 254, 502, 563, 622, 907, 952
CviQI GTAC 1 cut(s) 1022
DdeI CTNAG 3 cut(s) 59, 842, 945
DpnI GATC 6 cut(s) 252, 312, 346, 416, 477, 626
DpnII GATC 6 cut(s) 250, 310, 344, 414, 475, 624
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
EciI GGCGGA 1 cut(s) 764
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 3 cut(s) 45, 154, 404
Eco24I GRGCYC 2 cut(s) 914, 951
Eco31I GGTCTC 1 cut(s) 263
Eco32I GATATC 2 cut(s) 641, 1061
Eco47I GGWCC 2 cut(s) 81, 955
Eco53kI GAGCTC 1 cut(s) 949
EcoICRI GAGCTC 1 cut(s) 949
EcoRV GATATC 2 cut(s) 641, 1061
EcoT14I CCWWGG 3 cut(s) 45, 154, 404
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 914, 951
ErhI CCWWGG 3 cut(s) 45, 154, 404
FaeI CATG 9 cut(s) 49, 113, 211, 257, 505, 566, 625, 910, 955
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 1 cut(s) 418
FatI CATG 9 cut(s) 45, 109, 207, 253, 501, 562, 621, 906, 951
FbaI TGATCA 2 cut(s) 475, 624
Fnu4HI GCNGC 2 cut(s) 716, 897
FokI GGATG 3 cut(s) 122, 179, 760
FriOI GRGCYC 2 cut(s) 914, 951
Fsp4HI GCNGC 2 cut(s) 716, 897
FspBI CTAG 4 cut(s) 245, 351, 915, 1052
GluI GCNGC 2 cut(s) 716, 897
GsuI CTGGAG 2 cut(s) 287, 413
HaeIII GGCC 2 cut(s) 97, 573
Hin1II CATG 9 cut(s) 49, 113, 211, 257, 505, 566, 625, 910, 955
HincII GTYRAC 2 cut(s) 634, 871
HindII GTYRAC 2 cut(s) 634, 871
HindIII AAGCTT 1 cut(s) 822
HinfI GANTC 1 cut(s) 32
HpaI GTTAAC 1 cut(s) 871
HphI GGTGA 3 cut(s) 223, 485, 821
Hpy166II GTNNAC 3 cut(s) 84, 634, 871
Hpy188I TCNGA 2 cut(s) 691, 747
Hpy188III TCNNGA 7 cut(s) 67, 266, 383, 578, 907, 944, 1052
Hpy8I GTNNAC 3 cut(s) 84, 634, 871
HpyAV CCTTC 3 cut(s) 328, 584, 997
HpyCH4V TGCA 6 cut(s) 122, 128, 421, 521, 718, 856
HpyF3I CTNAG 3 cut(s) 59, 842, 945
Hsp92II CATG 9 cut(s) 49, 113, 211, 257, 505, 566, 625, 910, 955
Ksp22I TGATCA 2 cut(s) 475, 624
KspAI GTTAAC 1 cut(s) 871
Kzo9I GATC 6 cut(s) 250, 310, 344, 414, 475, 624
LmnI GCTCC 4 cut(s) 291, 322, 432, 681
Lsp1109I GCAGC 2 cut(s) 702, 908
LweI GCATC 1 cut(s) 115
MaeI CTAG 4 cut(s) 245, 351, 915, 1052
MaeIII GTNAC 3 cut(s) 33, 365, 809
MalI GATC 6 cut(s) 252, 312, 346, 416, 477, 626
MboI GATC 6 cut(s) 250, 310, 344, 414, 475, 624
MboII GAAGA 7 cut(s) 29, 101, 229, 305, 607, 617, 892
MfeI CAATTG 1 cut(s) 422
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 4 cut(s) 54, 914, 941, 951
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 9 cut(s) 12, 70, 102, 397, 422, 529, 787, 919, 1065
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 430
Mox20I TGGCCA 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 523
MscI TGGCCA 1 cut(s) 97
MseI TTAA 5 cut(s) 105, 296, 591, 704, 870
Msp20I TGGCCA 1 cut(s) 97
MunI CAATTG 1 cut(s) 422
Mva1269I GAATGC 1 cut(s) 523
NcoI CCATGG 1 cut(s) 45
NdeII GATC 6 cut(s) 250, 310, 344, 414, 475, 624
NheI GCTAGC 1 cut(s) 350
NlaIII CATG 9 cut(s) 49, 113, 211, 257, 505, 566, 625, 910, 955
NlaIV GGNNCC 2 cut(s) 683, 957
NmuCI GTSAC 2 cut(s) 33, 809
NsiI ATGCAT 1 cut(s) 523
PagI TCATGA 1 cut(s) 906
PctI GAATGC 1 cut(s) 523
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 2 cut(s) 717, 898
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Psp124BI GAGCTC 1 cut(s) 951
PspN4I GGNNCC 2 cut(s) 683, 957
PspPI GGNCC 3 cut(s) 81, 571, 955
PsrI GAACNNNNNNTAC 2 cut(s) 1005, 1037
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 1 cut(s) 1023
RsaNI GTAC 1 cut(s) 1022
SacI GAGCTC 1 cut(s) 951
SaqAI TTAA 5 cut(s) 105, 296, 591, 704, 870
SatI GCNGC 2 cut(s) 716, 897
Sau3AI GATC 6 cut(s) 250, 310, 344, 414, 475, 624
Sau96I GGNCC 3 cut(s) 81, 571, 955
SchI GAGTC 1 cut(s) 41
SduI GDGCHC 4 cut(s) 54, 914, 941, 951
SfaNI GCATC 1 cut(s) 115
SinI GGWCC 2 cut(s) 81, 955
Sse9I AATT 9 cut(s) 12, 70, 102, 397, 422, 529, 787, 919, 1065
SsiI CCGC 1 cut(s) 775
SspI AATATT 1 cut(s) 805
SspMI CTAG 4 cut(s) 245, 351, 915, 1052
SstI GAGCTC 1 cut(s) 951
StyI CCWWGG 3 cut(s) 45, 154, 404
TasI AATT 9 cut(s) 12, 70, 102, 397, 422, 529, 787, 919, 1065
Tru1I TTAA 5 cut(s) 105, 296, 591, 704, 870
Tru9I TTAA 5 cut(s) 105, 296, 591, 704, 870
TscAI CASTG 2 cut(s) 366, 870
TseFI GTSAC 2 cut(s) 33, 809
TseI GCWGC 2 cut(s) 715, 896
Tsp45I GTSAC 2 cut(s) 33, 809
TspDTI ATGAA 4 cut(s) 64, 274, 784, 895
TspRI CASTG 2 cut(s) 366, 870
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 81, 955
XapI RAATTY 3 cut(s) 70, 397, 787
XbaI TCTAGA 1 cut(s) 1051
XspI CTAG 4 cut(s) 245, 351, 915, 1052
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.