RchiOBHm_Chr7g0204791

Gibberellin 20 oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016855
Physical Location & Seq
Forward (+)
22441592 .. 22443116
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18331

Sequence Viewer

Length: 834 bp
ATGGCTTTGCAAATCGAAGAACAATACGTCGAGTCACTCTCCTCCATGGGACACAAACTAAGTGTCAAGAACTTCATTTGGTCCAAGGAAGAATGGCCAGCAATAAATCATGATGAGTTTACAGATTCACATGACATTCCTGCAATAAGTCTCCGAGAGATTTCAGATGGGAGAAGAAACCCTAATTATGAGAAGGTCTGCAAGGTCATGGTGAATGCTTGTGAGAAATGGGGGTTCTTCAAGTTAGTGGATCATGGGGTTGCTTTGGAGATCATAGAGAATTTCAAAGGGAGTATGAATGCGCTTTTTGATCTTCCAATGGAGCAGAAGCTGAAGGGTGTTAGATCAGCTAGTTTACCATTAGGTTACTGTGCATCAAATCCTGATTATGGGAAGAATTTGCCCTGGGCAGAGATCTTACAGTTGCTCCAGTCACCACAAATGGTGGTTGGATTTGCAACAAAGGTTTTTGGTGATCAACATCAGCCGTTCAGTGATGCAATGATTGGTTACTTGAATGCATTGGATAATCTGGGAATGACTATTTTGGAGATGCTAGCTCATGGATTGGGTCTTCCAGACAGCTTCTTCACAAAGAACTTTGAAGCAAAAGAGGCTACAATGATCAGAGTCAACAGATATCCTCCTTGTCCTCTTCCAGAAAAATGTCTTGGGCTGGGAAGCCATTCAGACCCTCATACCTTAACCATATTGTTACAAGATGATGTTGGTGGGCTTCAAGTTCTTATGAATGACAACAAATGGATTGGCATCCGTCCTGTTCAGAATTCTTTCATTATCAACATTGGTGACACCCTTGAGGTAATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

277

Amino Acids

31.02

Weight (kDa)

5.16

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 46 - 145 1.2e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 277 2e-19 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19970
malus_domestica MD04G1049700.v1.1 MD06G1050300.v1.1
prunus_persica Prupe.5G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204791
rosa_laevigata RLG00000003433
rosa_multiflora Rmu_sc0003221.1_g000048
rosa_roxburghii Rroxscaffold_3G00252820
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216000 Rh7BG213100 Rh7CG229300 Rh7DG223600
rosa_wichuraiana Rw7G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 3 cut(s) 280, 397, 787
AcuI CTGAAG 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 5 cut(s) 241, 286, 517, 605, 740
AjnI CCWGG 1 cut(s) 404
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
AluBI AGCT 4 cut(s) 331, 350, 560, 585
AluI AGCT 4 cut(s) 331, 350, 560, 585
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 258
AlwNI CAGNNNCTG 1 cut(s) 331
AoxI GGCC 1 cut(s) 95
ApoI RAATTY 3 cut(s) 280, 397, 787
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
Asp700I GAANNNNTTC 2 cut(s) 685, 791
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 4 cut(s) 223, 426, 485, 821
AsuNHI GCTAGC 1 cut(s) 556
AvaII GGWCC 1 cut(s) 81
BalI TGGCCA 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 566
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 1 cut(s) 406
BclI TGATCA 2 cut(s) 475, 624
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 2 cut(s) 351, 557
BglII AGATCT 1 cut(s) 414
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 4 cut(s) 383, 487, 543, 780
BmtI GCTAGC 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 566
BplI GAGNNNNNCTC 2 cut(s) 23, 55
BpmI CTGGAG 1 cut(s) 413
BsaBI GATNNNNATC 2 cut(s) 480, 770
BsaJI CCNNGG 4 cut(s) 45, 84, 404, 405
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 430
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 2 cut(s) 480, 770
BseBI CCWGG 1 cut(s) 406
BseDI CCNNGG 4 cut(s) 45, 84, 404, 405
BseGI GGATG 1 cut(s) 771
BseJI GATNNNNATC 2 cut(s) 480, 770
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 676
BshFI GGCC 1 cut(s) 97
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 63
BsmI GAATGC 3 cut(s) 220, 304, 523
BsnI GGCC 1 cut(s) 97
Bsp143I GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 1 cut(s) 97
BspHI TCATGA 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 560
BspPI GGATC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 4 cut(s) 45, 84, 404, 405
BssMI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
BssT1I CCWWGG 2 cut(s) 45, 84
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 2 cut(s) 371, 423
Bst6I CTCTTC 1 cut(s) 660
BstC8I GCNNGC 2 cut(s) 99, 558
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 771
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 7 cut(s) 253, 273, 313, 347, 417, 478, 627
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstNI CCWGG 1 cut(s) 406
BstSCI CCNGG 1 cut(s) 404
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 1 cut(s) 97
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 99, 558
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 81
CviAII CATG 6 cut(s) 46, 110, 131, 208, 254, 563
DdeI CTNAG 1 cut(s) 59
DpnI GATC 7 cut(s) 252, 272, 312, 346, 416, 477, 626
DpnII GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco130I CCWWGG 2 cut(s) 45, 84
Eco32I GATATC 1 cut(s) 641
Eco47I GGWCC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 1 cut(s) 404
EcoRV GATATC 1 cut(s) 641
EcoT14I CCWWGG 2 cut(s) 45, 84
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 2 cut(s) 45, 84
FaeI CATG 6 cut(s) 49, 113, 134, 211, 257, 566
FaqI GGGAC 1 cut(s) 63
FatI CATG 6 cut(s) 45, 109, 130, 207, 253, 562
FbaI TGATCA 2 cut(s) 475, 624
FokI GGATG 1 cut(s) 758
FspBI CTAG 2 cut(s) 351, 557
GlaI GCGC 1 cut(s) 303
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 413
HaeIII GGCC 1 cut(s) 97
HhaI GCGC 1 cut(s) 304
Hin1II CATG 6 cut(s) 49, 113, 134, 211, 257, 566
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 3 cut(s) 32, 125, 630
HphI GGTGA 4 cut(s) 223, 426, 485, 821
Hpy166II GTNNAC 3 cut(s) 120, 356, 634
Hpy188I TCNGA 5 cut(s) 155, 166, 629, 691, 786
Hpy188III TCNNGA 5 cut(s) 67, 110, 383, 578, 659
Hpy8I GTNNAC 3 cut(s) 120, 356, 634
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 187, 328
HpyCH4III ACNGT 2 cut(s) 371, 423
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 7 cut(s) 10, 143, 201, 374, 458, 500, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 6 cut(s) 49, 113, 134, 211, 257, 566
HspAI GCGC 1 cut(s) 302
Ksp22I TGATCA 2 cut(s) 475, 624
Kzo9I GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
LmnI GCTCC 2 cut(s) 322, 432
LweI GCATC 4 cut(s) 383, 487, 543, 780
MaeI CTAG 2 cut(s) 351, 557
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 6 cut(s) 33, 365, 432, 509, 714, 809
MalI GATC 7 cut(s) 252, 272, 312, 346, 416, 477, 626
MboI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
MboII GAAGA 9 cut(s) 29, 101, 186, 229, 305, 406, 566, 580, 647
MflI RGATCY 1 cut(s) 414
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 5 cut(s) 184, 280, 397, 787, 825
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 2 cut(s) 41, 639
MmeI TCCRAC 1 cut(s) 430
MnlI CCTC 6 cut(s) 52, 607, 654, 663, 705, 814
Mox20I TGGCCA 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 2 cut(s) 685, 791
MscI TGGCCA 1 cut(s) 97
MseI TTAA 2 cut(s) 704, 832
Msp20I TGGCCA 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 406
Mva1269I GAATGC 3 cut(s) 220, 304, 523
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 614
NcoI CCATGG 1 cut(s) 45
NdeII GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
NheI GCTAGC 1 cut(s) 556
NlaIII CATG 6 cut(s) 49, 113, 134, 211, 257, 566
NmuCI GTSAC 3 cut(s) 33, 432, 809
NsiI ATGCAT 1 cut(s) 523
PagI TCATGA 1 cut(s) 109
PasI CCCWGGG 1 cut(s) 405
PctI GAATGC 3 cut(s) 220, 304, 523
PdmI GAANNNNTTC 2 cut(s) 685, 791
PfeI GAWTC 1 cut(s) 125
PleI GAGTC 2 cut(s) 40, 638
PpsI GAGTC 2 cut(s) 40, 638
Psp6I CCWGG 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 1 cut(s) 404
PspPI GGNCC 1 cut(s) 81
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 414
SaqAI TTAA 2 cut(s) 704, 832
Sau3AI GATC 7 cut(s) 250, 270, 310, 344, 414, 475, 624
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 2 cut(s) 41, 639
ScrFI CCNGG 1 cut(s) 406
SfaNI GCATC 4 cut(s) 383, 487, 543, 780
SinI GGWCC 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
Sse9I AATT 5 cut(s) 184, 280, 397, 787, 825
SspMI CTAG 2 cut(s) 351, 557
StyD4I CCNGG 1 cut(s) 404
StyI CCWWGG 2 cut(s) 45, 84
TaaI ACNGT 2 cut(s) 371, 423
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 15, 30
TasI AATT 5 cut(s) 184, 280, 397, 787, 825
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 2 cut(s) 704, 832
Tru9I TTAA 2 cut(s) 704, 832
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 3 cut(s) 33, 432, 809
Tsp45I GTSAC 3 cut(s) 33, 432, 809
TspDTI ATGAA 4 cut(s) 64, 311, 764, 784
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 3 cut(s) 280, 397, 787
XmnI GAANNNNTTC 2 cut(s) 685, 791
XspI CTAG 2 cut(s) 351, 557
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.