MD04G1049700.v1.1

Gibberellin 20 oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016855
Physical Location & Seq
Forward (+)
5824579 .. 5825030
452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1049700.v1.1.491

Sequence Viewer

Length: 267 bp
ATGTCTCGGGCTTGGGAGCCATTCAGACCCACATACCTTTTAACTATATTGTTACAAGATGATGTTGGCGGCCTTCAAGTTCTGATGAGTGACAAGAAATGGGTTGGTATCCGTCCTGTTCAAAGTTCTTTCCTCAACATTGGTGACACCCTTGAACTGTATATGTTCTTGCAAGCTTGGACCAATTCTGCCCTCATTGACTGCCCTCTTAAGCTCATGGATTCAAAAATCAACCCTAGAAAGTATGAGCCCTTCACTTGGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

89

Amino Acids

10.21

Weight (kDa)

6.11

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 13 - 58 1.4e-07 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19970
malus_domestica MD04G1049700.v1.1 MD06G1050300.v1.1
prunus_persica Prupe.5G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204791
rosa_laevigata RLG00000003433
rosa_multiflora Rmu_sc0003221.1_g000048
rosa_roxburghii Rroxscaffold_3G00252820
rosa_rugosa Rorug07G0086000
rosa_samantha Rh7AG216000 Rh7BG213100 Rh7CG229300 Rh7DG223600
rosa_wichuraiana Rw7G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 258
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 77, 122, 155, 225
AluBI AGCT 2 cut(s) 176, 214
AluI AGCT 2 cut(s) 176, 214
Alw26I GTCTC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 180
BanII GRGCYC 1 cut(s) 252
BarI GAAGNNNNNNTAC 1 cut(s) 236
BciVI GTATCC 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 237
BfrI CTTAAG 1 cut(s) 209
BfuI GTATCC 1 cut(s) 119
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 180
BmiI GGNNCC 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 258
BshFI GGCC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 72
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 1 cut(s) 252
BspACI CCGC 1 cut(s) 69
BspANI GGCC 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 18
BspTI CTTAAG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 174
BstMAI GTCTC 1 cut(s) 9
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 180
CviAII CATG 1 cut(s) 217
CviJI RGCY 6 cut(s) 11, 19, 72, 176, 214, 250
CviKI_1 RGCY 6 cut(s) 11, 19, 72, 176, 214, 250
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 220
FaiI YATR 6 cut(s) 34, 47, 162, 164, 218, 246
FatI CATG 1 cut(s) 216
Fnu4HI GCNGC 1 cut(s) 70
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 70
FspBI CTAG 1 cut(s) 237
GluI GCNGC 1 cut(s) 70
HaeIII GGCC 1 cut(s) 72
Hin1II CATG 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 174
HinfI GANTC 1 cut(s) 221
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 26, 84
HpyAV CCTTC 2 cut(s) 83, 262
HpyCH4III ACNGT 1 cut(s) 159
HpyCH4V TGCA 1 cut(s) 172
Hsp92II CATG 1 cut(s) 220
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 1 cut(s) 129
MaeI CTAG 1 cut(s) 237
MaeIII GTNAC 3 cut(s) 51, 89, 143
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 1 cut(s) 184
MnlI CCTC 3 cut(s) 143, 203, 216
MseI TTAA 2 cut(s) 41, 210
MspCI CTTAAG 1 cut(s) 209
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 2 cut(s) 89, 143
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 41, 210
SatI GCNGC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 180
SduI GDGCHC 1 cut(s) 252
SetI ASST 3 cut(s) 39, 178, 216
SinI GGWCC 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 184
SsiI CCGC 1 cut(s) 69
SspMI CTAG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 159
TasI AATT 1 cut(s) 184
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 41, 210
Tru9I TTAA 2 cut(s) 41, 210
TseFI GTSAC 2 cut(s) 89, 143
Tsp45I GTSAC 2 cut(s) 89, 143
TspGWI ACGGA 1 cut(s) 101
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 180
XspI CTAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.