FvH4_5g20830

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014153
Physical Location & Seq
Reverse (-)
12409398 .. 12412898
3501 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20830.t2

Sequence Viewer

Length: 2367 bp
ATGGATGCAACTCACAAATTCGTATGCCAAGATGATTTGGCTAACCCACTAGCGTCCATGGCCATCCAAGTCTCTTGCATTCTCGTCATCTCACACTTCTTTCACATTTTGCTCAAGGCACTGGGTCAACCAGGTCCCATTGCTCAGATCCTAGCTGGTTTGGTGTTAGGTCCTTCGGGATTGTGTAACATAAAAGAAGTAAAGAATTACTTCTTACCGGCGTCAGCTGCACAATACTACGAGATGTTCGGGTTCTTCTGCCGCATCCTCTTCATGTTCTTGTTTGGTTTGGAGGTGGACATTGCTTATATTAGGCGAAACATTCGTGTTGTGTGCACAATTGCATGCGGTGGTGCCTCAATAGGAGCCATTTTTGGGTTGGTCTCCTCCTTCTTTTTGAGTAGTCACTTTTTGGAAAAACCCTCTGGCAAGGCATATTATGTTTTATCCATGATGTTATTTGTAGCCTACTCCGCCACCCCAGTCGTGAACCGTCTAGCAGCAGAGTTGAAATTCGCAACCTCAGACATTGGACGCTTGGCGGTGTCTTCTGCACTGGTCACTGAGCTTGCTTGCTTACTGGTATTCAATTTCATGAGCTTGTGCACGTCAGGGAAAATGGTAGGAAACGCCTTCTTAATCCTAGTAGGGTTGGTGCTTGTGCTCGTTGCTTTTAGGTACTTAGCTACATGGTTAAACAAACGCATCAATAACCACAAGTACCTTAGAAACCCTGAGGTGTTCCTCATACTGTCGGTTCTAATAGGGACTTCTATGCTGATCGAATTGGCCAACTTCAATAGCATCATAGCTTGCTTTCTCGCTGGCGTGGCATTCCCCAAAGAAGGGAAAACGGCAAGAACATTACTGCATAAACTCACTTACTCCGTTCACAACTTTGTTCTTCCTGTTTATTTTGGGTACATCGGGTTCCAATTCGATGGGGCTTATTTTGGTAACCTAACCAACATATTGATTGTTCTCATTCTGGTGCTGTTGTGCATCAGCAGCAAGATTGGTGGCGTTCTTGCAGTTTGCCACTATTTGAAGATTCCACTCAGTGAAGGGTTCTTCCTTGCTTTTGTATTGAACTTGAAGGGCCATGCTGATCTATTATTCATCGGCAGCGCGGGCAAACAACTATTTGAATGGACCCCTGTGGCTTACAATTTGTTAGTAATGACAATCGTTATTAGCACTGTAATATCAGGGCCAATGGTGGCTATCCTAATGAATAAAGATTATCAACTATTCGCTCACACACGCACCACTGTGAGTACTGAACTAGAAAAGCCTGAGAATGAGCTTCGAGTGCTGGGATGCGTGTATGGACCTCGTCATGTAAACGCTATACTATCGGTTATTGCAACACTTAGAGGTCCTCAAACAGCACCCATAATGCCCTACCTGGCTCACCTGATCGAGCTACAACGAAAACGCAGAACCAATGTGTCGTATCATGAGCTCGAAGATAATGAAATGAGCGATGAGGATGAGTACGGTGGTAATGACGTAGTGGAAATCCACAGAACAGTTGACACCTTCACTGTAGATACCAGCATTCTAATCAACATGATCAAAGCTGTGTCAACTTTATCTGGCCTGTATGAGGATGTGTGCACTGCTGCTGAGGATTTGCGCACGACTATCATCCTGCTGCCGTTTCATAAGCATCAGCGCATTGATGGGAAGATGGAGACTGGAAAAGAAGCTGTGAGGACGACGAACCAGAAGATTCTTCGGCATGCGCCTAGTACTGTTGGGATTATTGTAGAGAAAGGGCTAGCTGGGGCATTAGGGTTCTCGCAGCTCACTAGGGTGGACACTGTTCAACATGTTGCGACTCTTTTTTTTGGAGGGCCTGATGATCGTGAAGCTATTGCATGGAGTACTCGAATCGCAAATCATACGAAGATAAATTTGACGGTGATTAGATTTCTGCCTAGAGAATCATCAAACCCAAGGAATATGAAGGTAAACTCAGAGAAGCAGGGAAAGGATGACATTGAGGTCTTCATGGCGTTGTCAAGTTTGGAAATGGGCAATGACATTGACAATTCTTTTCTCAATGACTTCTATAACGGGTATGTTGCATCAGGTAAAGTTGGATACGTTGAGAAGTATACAAACAGTGGGGCAGAAACAGTGGCAGCTCTACGAGACATTGGGGACTTGTACACGCTGTTTATAGTAGGGCGGGGTGGGAGAGGGCACTCACCATTGACCACACAGATGAGTGATTGGGAAGAATGTCCAGAACTAGGAACTGTTGGAGATCTCTTGGCTTCTTCAGATTTCAACGTCAATGGTTCAATTTTGGTAGTTCAACAACATAGACACACGAAGAAAGACCTTATAGATGATTAG

Protein Analysis

789

Amino Acids

86.92

Weight (kDa)

6.63

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 30 - 413 3e-41 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 511 - 598 3.7e-11 Plant cation/H(+) antiporter domain
CHX17_C PF23259 613 - 652 8.4e-06 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16380 AT1G79400
fragaria_vesca FvH4_5g20830 FvH4_5g20830
malus_domestica MD06G1082400.v1.1
prunus_persica Prupe.5G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0207831
rosa_laevigata RLG00000003284
rosa_multiflora Rmu_sc0004647.1_g000005
rosa_roxburghii Rroxscaffold_3G00251120
rosa_rugosa Rorug07G0099700 Rorug07G0099800
rosa_samantha Rh7AG233000 Rh7BG228300 Rh7CG248000 Rh7DG239000
rosa_wichuraiana Rw7G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2009
Acc16I TGCGCA 1 cut(s) 1640
AccB1I GGYRCC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 2123
AccII CGCG 1 cut(s) 1130
AciI CCGC 6 cut(s) 262, 348, 474, 542, 1130, 2197
AclWI GGATC 1 cut(s) 142
AcoI YGGCCR 2 cut(s) 60, 789
AcsI RAATTY 3 cut(s) 17, 512, 1918
AcuI CTGAAG 1 cut(s) 2274
AcyI GRCGYC 1 cut(s) 221
AdeI CACNNNGTG 1 cut(s) 1061
AfaI GTAC 8 cut(s) 680, 722, 923, 1279, 1499, 1756, 1891, 2177
AfiI CCNNNNNNNGG 5 cut(s) 375, 845, 846, 1609, 2261
AflIII ACRYGT 1 cut(s) 1834
AjiI CACGTC 1 cut(s) 609
AjnI CCWGG 2 cut(s) 130, 1407
AleI CACNNNNGTG 2 cut(s) 1271, 1817
Alw21I GWGCWC 5 cut(s) 338, 608, 666, 1467, 1622
Alw26I GTCTC 4 cut(s) 76, 388, 1691, 2154
Alw44I GTGCAC 3 cut(s) 334, 604, 1618
AlwI GGATC 1 cut(s) 142
AoxI GGCC 6 cut(s) 60, 789, 1099, 1211, 1600, 1859
ApaLI GTGCAC 3 cut(s) 334, 604, 1618
ApeKI GCWGC 8 cut(s) 227, 500, 1008, 1125, 1625, 1657, 1807, 2150
ApoI RAATTY 3 cut(s) 17, 512, 1918
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 4 cut(s) 1130, 1641, 1680, 1750
AspS9I GGNCC 8 cut(s) 134, 170, 1099, 1152, 1211, 1331, 1379, 1859
AsuHPI GGTGA 3 cut(s) 1406, 1939, 2208
AsuNHI GCTAGC 1 cut(s) 1783
AvaII GGWCC 5 cut(s) 134, 170, 1152, 1331, 1379
AxyI CCTNAGG 1 cut(s) 735
BaeGI GKGCMC 4 cut(s) 338, 608, 1622, 2214
BalI TGGCCA 2 cut(s) 62, 791
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 1467
BbsI GAAGAC 2 cut(s) 540, 2005
Bbv12I GWGCWC 5 cut(s) 338, 608, 666, 1467, 1622
BbvCI CCTCAGC 1 cut(s) 1629
BbvI GCAGC 8 cut(s) 214, 512, 1020, 1137, 1612, 1644, 1819, 2162
BccI CCATC 4 cut(s) 71, 935, 1679, 1687
BceAI ACGGC 2 cut(s) 870, 1645
BciT130I CCWGG 2 cut(s) 132, 1409
BciVI GTATCC 1 cut(s) 2102
BclI TGATCA 1 cut(s) 1575
BcoDI GTCTC 4 cut(s) 76, 388, 1691, 2154
BfmI CTRYAG 1 cut(s) 1548
BfuI GTATCC 1 cut(s) 2102
BglII AGATCT 1 cut(s) 2275
BisI GCNGC 9 cut(s) 228, 262, 501, 1009, 1126, 1626, 1658, 1808, 2151
BlsI GCNGC 9 cut(s) 229, 263, 502, 1010, 1127, 1627, 1659, 1809, 2152
BmcAI AGTACT 3 cut(s) 1279, 1756, 1891
Bme1390I CCNGG 2 cut(s) 132, 1409
Bme18I GGWCC 5 cut(s) 134, 170, 1152, 1331, 1379
BmgBI CACGTC 1 cut(s) 609
BmgT120I GGNCC 8 cut(s) 134, 170, 1099, 1152, 1211, 1331, 1379, 1859
BmiI GGNNCC 5 cut(s) 136, 355, 367, 932, 1154
BmrFI CCNGG 2 cut(s) 132, 1409
BmrI ACTGGG 2 cut(s) 131, 476
BmsI GCATC 7 cut(s) 273, 714, 813, 1011, 1310, 1681, 2102
BmtI GCTAGC 1 cut(s) 1787
BmuI ACTGGG 2 cut(s) 131, 476
BpiI GAAGAC 2 cut(s) 540, 2005
Bpu10I CCTNAGC 1 cut(s) 1629
BpuEI CTTGAG 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 388
BsaJI CCNNGG 2 cut(s) 57, 1961
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 845, 846, 1609, 2261
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 5 cut(s) 126, 482, 561, 585, 1705
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 3 cut(s) 138, 300, 2050
BseBI CCWGG 2 cut(s) 132, 1409
BseDI CCNNGG 2 cut(s) 57, 1961
BseGI GGATG 8 cut(s) 10, 63, 264, 1325, 1498, 1618, 1650, 2005
BseLI CCNNNNNNNGG 5 cut(s) 375, 845, 846, 1609, 2261
BseMI GCAATG 3 cut(s) 138, 300, 2050
BseMII CTCAG 8 cut(s) 158, 537, 555, 726, 1072, 1287, 1620, 1995
BseNI ACTGG 5 cut(s) 126, 482, 561, 585, 1705
BseRI GAGGAG 1 cut(s) 376
BseSI GKGCMC 4 cut(s) 338, 608, 1622, 2214
BseXI GCAGC 8 cut(s) 214, 512, 1020, 1137, 1612, 1644, 1819, 2162
BseYI CCCAGC 2 cut(s) 1315, 1787
BsgI GTGCAG 2 cut(s) 213, 537
Bsh1236I CGCG 1 cut(s) 1130
BshFI GGCC 6 cut(s) 62, 791, 1101, 1213, 1602, 1861
BshNI GGYRCC 1 cut(s) 353
BsiHKAI GWGCWC 5 cut(s) 338, 608, 666, 1467, 1622
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 3 cut(s) 120, 781, 2183
BslI CCNNNNNNNGG 5 cut(s) 375, 845, 846, 1609, 2261
BsmAI GTCTC 4 cut(s) 76, 388, 1691, 2154
BsmFI GGGAC 3 cut(s) 120, 781, 2183
BsmI GAATGC 3 cut(s) 78, 833, 1560
BsnI GGCC 6 cut(s) 62, 791, 1101, 1213, 1602, 1861
Bso31I GGTCTC 1 cut(s) 388
Bsp1286I GDGCHC 6 cut(s) 338, 608, 666, 1467, 1622, 2214
Bsp1407I TGTACA 1 cut(s) 2175
Bsp143I GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 6 cut(s) 262, 348, 474, 542, 1130, 2197
BspANI GGCC 6 cut(s) 62, 791, 1101, 1213, 1602, 1861
BspCNI CTCAG 8 cut(s) 157, 536, 556, 727, 1071, 1288, 1621, 1994
BspFNI CGCG 1 cut(s) 1130
BspHI TCATGA 2 cut(s) 594, 1459
BspLI GGNNCC 5 cut(s) 136, 355, 367, 932, 1154
BspOI GCTAGC 1 cut(s) 1787
BspPI GGATC 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 388
BsrDI GCAATG 3 cut(s) 138, 300, 2050
BsrFI RCCGGY 1 cut(s) 217
BsrGI TGTACA 1 cut(s) 2175
BsrI ACTGG 5 cut(s) 126, 482, 561, 585, 1705
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 57, 1961
BssMI GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
BssNAI GTATAC 1 cut(s) 2124
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 2 cut(s) 57, 1961
Bst1107I GTATAC 1 cut(s) 2124
Bst2UI CCWGG 2 cut(s) 132, 1409
Bst6I CTCTTC 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 221
BstAUI TGTACA 1 cut(s) 2175
BstC8I GCNNGC 8 cut(s) 346, 570, 574, 814, 826, 1132, 1746, 1785
BstDSI CCRYGG 1 cut(s) 57
BstEII GGTNACC 1 cut(s) 956
BstENI CCTNNNNNAGG 1 cut(s) 1607
BstF5I GGATG 8 cut(s) 10, 63, 264, 1325, 1498, 1618, 1650, 2005
BstFNI CGCG 1 cut(s) 1130
BstHHI GCGC 4 cut(s) 1130, 1641, 1680, 1750
BstKTI GATC 7 cut(s) 150, 783, 1111, 1422, 1578, 1870, 2278
BstMAI GTCTC 4 cut(s) 76, 388, 1691, 2154
BstMBI GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
BstMWI GCNNNNNNNGC 7 cut(s) 59, 227, 473, 830, 1008, 1131, 1312
BstNI CCWGG 2 cut(s) 132, 1409
BstNSI RCATGY 3 cut(s) 348, 1748, 1838
BstPI GGTNACC 1 cut(s) 956
BstSCI CCNGG 2 cut(s) 130, 1407
BstSFI CTRYAG 1 cut(s) 1548
BstSLI GKGCMC 4 cut(s) 338, 608, 1622, 2214
BstUI CGCG 1 cut(s) 1130
BstV1I GCAGC 8 cut(s) 214, 512, 1020, 1137, 1612, 1644, 1819, 2162
BstV2I GAAGAC 2 cut(s) 540, 2005
BstX2I RGATCY 2 cut(s) 147, 2275
BstXI CCANNNNNNTGG 1 cut(s) 941
BstYI RGATCY 2 cut(s) 147, 2275
BstZ17I GTATAC 1 cut(s) 2124
Bsu36I CCTNAGG 1 cut(s) 735
BsuI GTATCC 1 cut(s) 2102
BsuRI GGCC 6 cut(s) 62, 791, 1101, 1213, 1602, 1861
BtgI CCRYGG 1 cut(s) 57
BtgZI GCGATG 1 cut(s) 1500
BtrI CACGTC 1 cut(s) 609
BtsCI GGATG 8 cut(s) 10, 63, 264, 1325, 1498, 1618, 1650, 2005
BtsI GCAGTG 1 cut(s) 1620
Cac8I GCNNGC 8 cut(s) 346, 570, 574, 814, 826, 1132, 1746, 1785
CciI TCATGA 2 cut(s) 594, 1459
CfoI GCGC 4 cut(s) 1130, 1641, 1680, 1750
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 8 cut(s) 134, 170, 1099, 1152, 1211, 1331, 1379, 1859
CseI GACGC 3 cut(s) 42, 210, 543
CsiI ACCWGGT 1 cut(s) 130
Csp6I GTAC 8 cut(s) 679, 721, 922, 1278, 1498, 1755, 1890, 2176
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviQI GTAC 8 cut(s) 679, 721, 922, 1278, 1498, 1755, 1890, 2176
DpnI GATC 7 cut(s) 149, 782, 1110, 1421, 1577, 1869, 2277
DpnII GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
DraIII CACNNNGTG 1 cut(s) 1061
DrdI GACNNNNNNGTC 1 cut(s) 2009
DseDI GACNNNNNNGTC 1 cut(s) 2009
EaeI YGGCCR 2 cut(s) 60, 789
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
EciI GGCGGA 1 cut(s) 463
Ecl136II GAGCTC 1 cut(s) 1465
Eco130I CCWWGG 2 cut(s) 57, 1961
Eco24I GRGCYC 1 cut(s) 1467
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 5 cut(s) 134, 170, 1152, 1331, 1379
Eco53kI GAGCTC 1 cut(s) 1465
Eco57I CTGAAG 1 cut(s) 2274
Eco81I CCTNAGG 1 cut(s) 735
Eco91I GGTNACC 1 cut(s) 956
EcoICRI GAGCTC 1 cut(s) 1465
EcoNI CCTNNNNNAGG 1 cut(s) 1607
EcoO109I RGGNCCY 4 cut(s) 134, 170, 1379, 1859
EcoO65I GGTNACC 1 cut(s) 956
EcoRII CCWGG 2 cut(s) 130, 1407
EcoT14I CCWWGG 2 cut(s) 57, 1961
EcoT38I GRGCYC 1 cut(s) 1467
ErhI CCWWGG 2 cut(s) 57, 1961
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 3 cut(s) 120, 781, 2183
FauI CCCGC 2 cut(s) 1123, 2190
FbaI TGATCA 1 cut(s) 1575
FblI GTMKAC 1 cut(s) 2123
Fnu4HI GCNGC 9 cut(s) 228, 262, 501, 1009, 1126, 1626, 1658, 1808, 2151
FokI GGATG 8 cut(s) 17, 50, 251, 1332, 1505, 1625, 1637, 2012
FriOI GRGCYC 1 cut(s) 1467
Fsp4HI GCNGC 9 cut(s) 228, 262, 501, 1009, 1126, 1626, 1658, 1808, 2151
FspI TGCGCA 1 cut(s) 1640
GlaI GCGC 4 cut(s) 1129, 1640, 1679, 1749
GluI GCNGC 9 cut(s) 228, 262, 501, 1009, 1126, 1626, 1658, 1808, 2151
GsaI CCCAGC 2 cut(s) 1319, 1791
HaeIII GGCC 6 cut(s) 62, 791, 1101, 1213, 1602, 1861
HapII CCGG 1 cut(s) 218
HgaI GACGC 3 cut(s) 42, 210, 543
HhaI GCGC 4 cut(s) 1130, 1641, 1680, 1750
Hin1I GRCGYC 1 cut(s) 221
Hin6I GCGC 4 cut(s) 1128, 1639, 1678, 1748
HinP1I GCGC 4 cut(s) 1128, 1639, 1678, 1748
HincII GTYRAC 3 cut(s) 128, 1537, 1590
HindII GTYRAC 3 cut(s) 128, 1537, 1590
HinfI GANTC 5 cut(s) 1051, 1735, 1843, 1896, 1949
HpaII CCGG 1 cut(s) 218
HphI GGTGA 3 cut(s) 1406, 1939, 2208
Hpy188I TCNGA 4 cut(s) 147, 526, 1984, 2293
Hpy188III TCNNGA 6 cut(s) 177, 487, 595, 1460, 1871, 2255
Hpy99I CGWCG 1 cut(s) 1726
HpyAV CCTTC 8 cut(s) 183, 400, 643, 839, 1058, 1090, 1552, 1966
HpyCH4IV ACGT 4 cut(s) 608, 1512, 2112, 2301
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 227, 473, 830, 1008, 1131, 1312
HpySE526I ACGT 4 cut(s) 608, 1512, 2112, 2301
Hsp92I GRCGYC 1 cut(s) 221
HspAI GCGC 4 cut(s) 1128, 1639, 1678, 1748
Ksp22I TGATCA 1 cut(s) 1575
Kzo9I GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
LmnI GCTCC 1 cut(s) 365
Lsp1109I GCAGC 8 cut(s) 214, 512, 1020, 1137, 1612, 1644, 1819, 2162
LweI GCATC 7 cut(s) 273, 714, 813, 1011, 1310, 1681, 2102
MabI ACCWGGT 1 cut(s) 130
MaeII ACGT 4 cut(s) 608, 1512, 2112, 2301
MaeIII GTNAC 4 cut(s) 185, 404, 559, 956
MalI GATC 7 cut(s) 149, 782, 1110, 1421, 1577, 1869, 2277
MboI GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 2 cut(s) 147, 2275
MhlI GDGCHC 6 cut(s) 338, 608, 666, 1467, 1622, 2214
MlsI TGGCCA 2 cut(s) 62, 791
MluNI TGGCCA 2 cut(s) 62, 791
MlyI GAGTC 1 cut(s) 1837
MmeI TCCRAC 2 cut(s) 2086, 2251
Mox20I TGGCCA 2 cut(s) 62, 791
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 2 cut(s) 62, 791
MseI TTAA 2 cut(s) 638, 695
MslI CAYNNNNRTG 4 cut(s) 989, 1271, 1817, 2231
Msp20I TGGCCA 2 cut(s) 62, 791
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 2 cut(s) 132, 1409
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 3 cut(s) 78, 833, 1560
MvaI CCWGG 2 cut(s) 132, 1409
MvnI CGCG 1 cut(s) 1130
MwoI GCNNNNNNNGC 7 cut(s) 59, 227, 473, 830, 1008, 1131, 1312
NcoI CCATGG 1 cut(s) 57
NdeII GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
NheI GCTAGC 1 cut(s) 1783
NlaIV GGNNCC 5 cut(s) 136, 355, 367, 932, 1154
NmuCI GTSAC 2 cut(s) 404, 559
NsbI TGCGCA 1 cut(s) 1640
NspI RCATGY 3 cut(s) 348, 1748, 1838
OliI CACNNNNGTG 2 cut(s) 1271, 1817
PaeI GCATGC 2 cut(s) 348, 1748
PagI TCATGA 2 cut(s) 594, 1459
PciI ACATGT 1 cut(s) 1834
PctI GAATGC 3 cut(s) 78, 833, 1560
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 4 cut(s) 1051, 1735, 1896, 1949
PflFI GACNNNGTC 1 cut(s) 1335
PkrI GCNGC 9 cut(s) 229, 263, 502, 1010, 1127, 1627, 1659, 1809, 2152
PleI GAGTC 1 cut(s) 1837
PpsI GAGTC 1 cut(s) 1837
PpuMI RGGWCCY 3 cut(s) 134, 170, 1379
PscI ACATGT 1 cut(s) 1834
Psp124BI GAGCTC 1 cut(s) 1467
Psp5II RGGWCCY 3 cut(s) 134, 170, 1379
Psp6I CCWGG 2 cut(s) 130, 1407
PspEI GGTNACC 1 cut(s) 956
PspFI CCCAGC 2 cut(s) 1315, 1787
PspGI CCWGG 2 cut(s) 130, 1407
PspN4I GGNNCC 5 cut(s) 136, 355, 367, 932, 1154
PspPI GGNCC 8 cut(s) 134, 170, 1099, 1152, 1211, 1331, 1379, 1859
PspPPI RGGWCCY 3 cut(s) 134, 170, 1379
PsrI GAACNNNNNNTAC 2 cut(s) 914, 946
PsuI RGATCY 2 cut(s) 147, 2275
PsyI GACNNNGTC 1 cut(s) 1335
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 8 cut(s) 680, 722, 923, 1279, 1499, 1756, 1891, 2177
RsaNI GTAC 8 cut(s) 679, 721, 922, 1278, 1498, 1755, 1890, 2176
RseI CAYNNNNRTG 4 cut(s) 989, 1271, 1817, 2231
SacI GAGCTC 1 cut(s) 1467
SaqAI TTAA 2 cut(s) 638, 695
SatI GCNGC 9 cut(s) 228, 262, 501, 1009, 1126, 1626, 1658, 1808, 2151
Sau3AI GATC 7 cut(s) 147, 780, 1108, 1419, 1575, 1867, 2275
Sau96I GGNCC 8 cut(s) 134, 170, 1099, 1152, 1211, 1331, 1379, 1859
ScaI AGTACT 3 cut(s) 1279, 1756, 1891
SchI GAGTC 1 cut(s) 1837
ScrFI CCNGG 2 cut(s) 132, 1409
SduI GDGCHC 6 cut(s) 338, 608, 666, 1467, 1622, 2214
SexAI ACCWGGT 1 cut(s) 130
SfaNI GCATC 7 cut(s) 273, 714, 813, 1011, 1310, 1681, 2102
SfcI CTRYAG 1 cut(s) 1548
SinI GGWCC 5 cut(s) 134, 170, 1152, 1331, 1379
SmiMI CAYNNNNRTG 4 cut(s) 989, 1271, 1817, 2231
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SphI GCATGC 2 cut(s) 348, 1748
SsiI CCGC 6 cut(s) 262, 348, 474, 542, 1130, 2197
SstI GAGCTC 1 cut(s) 1467
StyD4I CCNGG 2 cut(s) 130, 1407
StyI CCWWGG 2 cut(s) 57, 1961
TaiI ACGT 4 cut(s) 611, 1515, 2115, 2304
TaqI TCGA 6 cut(s) 783, 939, 1309, 1422, 1467, 1894
TatI WGTACW 4 cut(s) 1277, 1754, 1889, 2175
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 4 cut(s) 1051, 1735, 1896, 1949
Tru1I TTAA 2 cut(s) 638, 695
Tru9I TTAA 2 cut(s) 638, 695
TseFI GTSAC 2 cut(s) 404, 559
TseI GCWGC 8 cut(s) 227, 500, 1008, 1125, 1625, 1657, 1807, 2150
Tsp45I GTSAC 2 cut(s) 404, 559
TspDTI ATGAA 8 cut(s) 262, 583, 1108, 1247, 1491, 1655, 1985, 2005
TspGWI ACGGA 1 cut(s) 877
Tth111I GACNNNGTC 1 cut(s) 1335
VneI GTGCAC 3 cut(s) 334, 604, 1618
VpaK11BI GGWCC 5 cut(s) 134, 170, 1152, 1331, 1379
XagI CCTNNNNNAGG 1 cut(s) 1607
XapI RAATTY 3 cut(s) 17, 512, 1918
XceI RCATGY 3 cut(s) 348, 1748, 1838
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 2123
XmnI GAANNNNTTC 1 cut(s) 209
ZrmI AGTACT 3 cut(s) 1279, 1756, 1891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.