MD06G1082400.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014153
Physical Location & Seq
Reverse (-)
20266924 .. 20270316
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1082400.v1.1.491

Sequence Viewer

Length: 2370 bp
ATGGAGGCTACTCACAAATTCGTATGCCAAGACGATTTGGTCAACCCTCTATCCTCCATGGGCATGCAAGTAGCTTGCATCCTTGTCATCTCACATTTCTTTCACATTCTGCTCAAGGCCTTGGGACAACCAGGCCCCATTGCTCAGATTCTTGCTGGTTTGGTGTTGGGTCCGTCAGGGTTGTCTAATATTTCTGAAATAAAAGAATTCTTCTTTCAAGCTTCTGCTGCAGAATACTACGAGATGTTCGGGTTCTTCTGCCGGATTCTCTTCATGTTCCTATTCGGTTTGGAGATAGACATTGCGTATATACGCCACAACCTACGCATTGTGTGTATCGTTGCCTACGGTGGTGCCTCAGTAGGCGGCATTTTTGGCCTTGTTGCCTCCTTCTTTATCTCCAAGCACTTAATGGCTGAAAATGCCAAACTGAACGCTAGTTTCGTTTTTTGCATCATGTTACTTGTAGCGTACACATCTTCACCAGTTGTCAATCGCTTAGGAGCAGAGTTAAGGTTTTCAACTTCTGACATTGGTCGCTTGGCAGCATCTGCAGCTATGGTCGTCGAACTTACCTGCTTACTTGTTTTCAATTTGATCATCGCGTTCTCAAAAGTTACTAATATACGGGACGGCTTCTTGATCCTCCTAGGCATGGTTCCAATACTTTTTGGGTTCAAGTACTTGGCAGTCTGGTTGAACAAACGTACCAATAAGCAAAAGTACCTCAGAAACCCTGAGGTGTTTCTCATTTTATCTATTCTAATAGCTGGTTCAATGATCATTGAAATGCGCACGTTTAATAGTGTGATAGCTTGTTTTGTTGCCGGCATGGTGTTCCCCAAGGAAGGCAAAACGGCAAGAACTTTGTTGCACAAACTTACTTACTCTGTCCACAACTTTGTACTCCCAGTTTACTTCGGCTATATTGGCTTCCAATTTGATGCGAGTCATTTGAAAAGCTGGAGAAATGTACTGATTGTGTTCATACTTGTGCTGTTGAGCCTCAGTAGCAAGATTGGTGGAACTCTTGCGGTTTGCCACTATTTGAAGATTCCACCAAATGAGGGGCTTTTCCTTGGTTTTGTCTTGAGCTTGAAGGGACATGCTGATATCTTGTTTGTTGGCAGCGCCTCAAAGGCACTAGTTACTTGGAACCCGACGGCTTACAACTTGTTACTAATGACAATAGTGATTAACACCGTAATATCAGGGCCAGTTGTGGCTTTATTAATGAAAAGAGAAGAAAGACTCTTCACTCACACACACACATCCCTTGGCTTTGAGCTGGAAAAGCCGGATCAGAGTGAGCTTCGACTGCTGGCCTGTGTGTACGGCCCTCGCCACATGTCAGCCATACTCTCGGTCATCGCCTCAATGCGGGGCTCCCAAACAGCCTCCATCATGCCCTACATGGCCCACCTGATCGAGCTAAATCGGAAACGCCGCACCAACGTGTCGTATCACGAGCTGGAGGCTGACGAAATGAGCGACGAAGAAGAGTACGGCGGAAACGATGTGCTCGAAATCCACGCAGCTGTGGATGCCTTCACTGCTGAGACTCGAATCCTAATCAACCTCAATAAGGCCGTCTCGACAGTGTCGAATTTGCATGAGGACGTGTGCAGCGCTGCCGAGGACTTGCGTGCGACTATCATTTTATTGCCCTTTCACAAGCATCAAAGAATCGACGGGAAAATGGAATCTGGGAAAGAGGCGGTGCGGACGACAAACCAAAAAATTCTTCGTCATGCTCCGAGTTCCGTTGGGATTATTGTCGAAAAGGGGCTTGCTGGGGCGCTAGGGTTTTCGCAGTTGTTCACGGTGGACGTTGTGCAACATGTTGCAACTCTCTTCTTTGGCGGACCGGATGACCGCGAGGCAATTGCTTGGAGTACAAGGATCGCGAATCATCCTAGAGTTAATTTGACAGTGATTAGGTTTCTGCCAGCAGAATCGTCGAATACGAGGAATATGAAGGTGGAGAATGAACTGGGGAATAATGACATTGAAGTTTTCATGGCTTTGTCGAGTTTAGAAACCGGGAATGACATTGACAATGCTTTTCTCAATGACTTTTATAATGGGTATGTGGCATCAGGTAAAGTTGGGTATGTTGAAAAGCAGGTGAACAATGGAGCAGAAACAGTGGCAGCTCTAAGAGACATAGGGGACTTGTACTCTCTTTTTATAGTAGGGAAGGGTGGACGAGGACACTCTCCATTGACAACTGAACTAAGCGACTGGGAAGAGTGCCCTGAATTGGGAACTGTTGGGGATCTCTTGGCTTCTTCAGATCTTAATATAAATGGTTCAATTTTGGTCGTTCAACAACATAGGAATTCCAAGAAAAACCTTATAGATGATTAG

Protein Analysis

790

Amino Acids

86.97

Weight (kDa)

6.4

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 30 - 413 3.2e-38 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 511 - 600 4.6e-13 Plant cation/H(+) antiporter domain
CHX17_C PF23259 615 - 779 1.7e-15 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16380 AT1G79400
fragaria_vesca FvH4_5g20830 FvH4_5g20830
malus_domestica MD06G1082400.v1.1
prunus_persica Prupe.5G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0207831
rosa_laevigata RLG00000003284
rosa_multiflora Rmu_sc0004647.1_g000005
rosa_roxburghii Rroxscaffold_3G00251120
rosa_rugosa Rorug07G0099700 Rorug07G0099800
rosa_samantha Rh7AG233000 Rh7BG228300 Rh7CG248000 Rh7DG239000
rosa_wichuraiana Rw7G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2082
AarI CACCTGC 1 cut(s) 2116
AasI GACNNNNNNGTC 1 cut(s) 38
Acc16I TGCGCA 1 cut(s) 794
Acc36I ACCTGC 2 cut(s) 584, 2116
AccB1I GGYRCC 1 cut(s) 353
AccII CGCG 3 cut(s) 605, 1878, 1907
AciI CCGC 9 cut(s) 366, 1034, 1381, 1447, 1509, 1718, 1723, 1863, 1876
AclWI GGATC 4 cut(s) 637, 1308, 1910, 2286
AcsI RAATTY 5 cut(s) 17, 206, 1606, 1740, 2341
AcuI CTGAAG 1 cut(s) 2277
AfeI AGCGCT 1 cut(s) 1630
AfiI CCNNNNNNNGG 7 cut(s) 655, 848, 1067, 1380, 1585, 2263, 2264
AflIII ACRYGT 4 cut(s) 1347, 1455, 1620, 1840
AhlI ACTAGT 1 cut(s) 1144
AjiI CACGTC 1 cut(s) 1621
AjnI CCWGG 1 cut(s) 130
AjuI GAANNNNNNNTTGG 2 cut(s) 1728, 1760
AleI CACNNNNGTG 1 cut(s) 1454
Alw21I GWGCWC 1 cut(s) 1524
Alw26I GTCTC 3 cut(s) 1553, 1597, 2157
AlwI GGATC 4 cut(s) 637, 1308, 1910, 2286
AlwNI CAGNNNCTG 1 cut(s) 551
Aor51HI AGCGCT 1 cut(s) 1630
AoxI GGCC 8 cut(s) 117, 133, 376, 1214, 1323, 1336, 1416, 1587
ApeKI GCWGC 8 cut(s) 227, 545, 554, 1128, 1535, 1626, 1631, 2153
ApoI RAATTY 5 cut(s) 17, 206, 1606, 1740, 2341
AseI ATTAAT 1 cut(s) 1232
AspA2I CCTAGG 1 cut(s) 649
AspLEI GCGC 4 cut(s) 795, 1133, 1631, 1801
AspS9I GGNCC 6 cut(s) 134, 170, 1214, 1337, 1417, 1865
AsuC2I CCSGG 1 cut(s) 2044
AsuHPI GGTGA 2 cut(s) 474, 2140
AvaII GGWCC 2 cut(s) 170, 1865
AvrII CCTAGG 1 cut(s) 649
AxyI CCTNAGG 1 cut(s) 738
BaeGI GKGCMC 1 cut(s) 2258
BaeI ACNNNNGTAYC 2 cut(s) 691, 724
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 1388
BauI CACGAG 1 cut(s) 1466
Bbv12I GWGCWC 1 cut(s) 1524
BbvI GCAGC 8 cut(s) 214, 557, 566, 1140, 1547, 1618, 1638, 2165
BccI CCATC 1 cut(s) 1409
BceAI ACGGC 6 cut(s) 649, 873, 1179, 1351, 1522, 1574
BcgI CGANNNNNNTGC 4 cut(s) 1868, 1902, 1941, 1975
BciT130I CCWGG 1 cut(s) 132
BclI TGATCA 2 cut(s) 597, 780
BcnI CCSGG 1 cut(s) 2044
BcoDI GTCTC 3 cut(s) 1553, 1597, 2157
BcuI ACTAGT 1 cut(s) 1144
BfaI CTAG 5 cut(s) 438, 650, 1145, 1802, 1917
BfmI CTRYAG 2 cut(s) 228, 552
BfoI RGCGCY 3 cut(s) 1134, 1632, 1802
BfuAI ACCTGC 2 cut(s) 584, 2116
BglI GCCNNNNNGGC 1 cut(s) 1139
BglII AGATCT 1 cut(s) 2296
BlnI CCTAGG 1 cut(s) 649
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 2 cut(s) 132, 2044
Bme18I GGWCC 2 cut(s) 170, 1865
BmgBI CACGTC 1 cut(s) 1621
BmgT120I GGNCC 6 cut(s) 134, 170, 1214, 1337, 1417, 1865
BmiI GGNNCC 6 cut(s) 136, 171, 355, 660, 1157, 1387
BmrFI CCNGG 2 cut(s) 132, 2044
BmrI ACTGGG 3 cut(s) 905, 2003, 2254
BmsI GCATC 7 cut(s) 87, 462, 557, 934, 1534, 1687, 2105
BmuI ACTGGG 3 cut(s) 905, 2003, 2254
BoxI GACNNNNGTC 1 cut(s) 534
BpmI CTGGAG 2 cut(s) 985, 1493
Bpu10I CCTNAGC 1 cut(s) 499
BpuEI CTTGAG 2 cut(s) 98, 1111
BpuMI CCSGG 1 cut(s) 2044
BsaJI CCNNGG 7 cut(s) 57, 120, 649, 843, 1078, 1276, 1635
BsaWI WCCGGW 1 cut(s) 1867
Bsc4I CCNNNNNNNGG 7 cut(s) 655, 848, 1067, 1380, 1585, 2263, 2264
Bse118I RCCGGY 1 cut(s) 827
Bse1I ACTGG 5 cut(s) 485, 911, 1217, 1998, 2249
Bse21I CCTNAGG 1 cut(s) 738
Bse3DI GCAATG 2 cut(s) 138, 300
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 7 cut(s) 57, 120, 649, 843, 1078, 1276, 1635
BseGI GGATG 5 cut(s) 78, 1271, 1549, 1876, 1912
BseLI CCNNNNNNNGG 7 cut(s) 655, 848, 1067, 1380, 1585, 2263, 2264
BseMI GCAATG 2 cut(s) 138, 300
BseMII CTCAG 6 cut(s) 158, 372, 729, 742, 1021, 1548
BseNI ACTGG 5 cut(s) 485, 911, 1217, 1998, 2249
BseSI GKGCMC 1 cut(s) 2258
BseXI GCAGC 8 cut(s) 214, 557, 566, 1140, 1547, 1618, 1638, 2165
BseYI CCCAGC 1 cut(s) 1793
BsgI GTGCAG 1 cut(s) 1645
Bsh1236I CGCG 3 cut(s) 605, 1878, 1907
BshFI GGCC 8 cut(s) 119, 135, 378, 1216, 1325, 1338, 1418, 1589
BshNI GGYRCC 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 1524
BsiSI CCGG 5 cut(s) 262, 828, 1298, 1868, 2043
BslFI GGGAC 4 cut(s) 138, 644, 1116, 2186
BslI CCNNNNNNNGG 7 cut(s) 655, 848, 1067, 1380, 1585, 2263, 2264
BsmAI GTCTC 3 cut(s) 1553, 1597, 2157
BsmBI CGTCTC 1 cut(s) 1597
BsmFI GGGAC 4 cut(s) 138, 644, 1116, 2186
BsnI GGCC 8 cut(s) 119, 135, 378, 1216, 1325, 1338, 1418, 1589
Bsp1286I GDGCHC 3 cut(s) 1388, 1524, 2258
Bsp143I GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
Bsp19I CCATGG 1 cut(s) 57
Bsp68I TCGCGA 1 cut(s) 1907
BspACI CCGC 9 cut(s) 366, 1034, 1381, 1447, 1509, 1718, 1723, 1863, 1876
BspANI GGCC 8 cut(s) 119, 135, 378, 1216, 1325, 1338, 1418, 1589
BspCNI CTCAG 6 cut(s) 157, 371, 730, 741, 1020, 1549
BspFNI CGCG 3 cut(s) 605, 1878, 1907
BspLI GGNNCC 6 cut(s) 136, 171, 355, 660, 1157, 1387
BspMAI CTGCAG 2 cut(s) 232, 556
BspMI ACCTGC 2 cut(s) 584, 2116
BspPI GGATC 4 cut(s) 637, 1308, 1910, 2286
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 138, 300
BsrFI RCCGGY 1 cut(s) 827
BsrI ACTGG 5 cut(s) 485, 911, 1217, 1998, 2249
BssAI RCCGGY 1 cut(s) 827
BssECI CCNNGG 7 cut(s) 57, 120, 649, 843, 1078, 1276, 1635
BssMI GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
BssSI CACGAG 1 cut(s) 1466
BssT1I CCWWGG 6 cut(s) 57, 120, 649, 843, 1078, 1276
Bst2BI CACGAG 1 cut(s) 1466
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 7 cut(s) 350, 1204, 1600, 1825, 1933, 2149, 2272
Bst6I CTCTTC 5 cut(s) 275, 1259, 1494, 1859, 2244
BstAPI GCANNNNNTGC 1 cut(s) 551
BstC8I GCNNGC 7 cut(s) 65, 76, 829, 1323, 1647, 1791, 1950
BstDEI CTNAG 9 cut(s) 144, 358, 499, 728, 738, 1007, 1557, 2159, 2237
BstDSI CCRYGG 1 cut(s) 57
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 5 cut(s) 78, 1271, 1549, 1876, 1912
BstFNI CGCG 3 cut(s) 605, 1878, 1907
BstH2I RGCGCY 3 cut(s) 1134, 1632, 1802
BstHHI GCGC 4 cut(s) 795, 1133, 1631, 1801
BstKTI GATC 8 cut(s) 600, 645, 783, 1303, 1428, 1905, 2281, 2299
BstMAI GTCTC 3 cut(s) 1553, 1597, 2157
BstMBI GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 4 cut(s) 67, 1109, 1351, 1844
BstPAI GACNNNNGTC 1 cut(s) 534
BstSCI CCNGG 2 cut(s) 130, 2042
BstSFI CTRYAG 2 cut(s) 228, 552
BstSLI GKGCMC 1 cut(s) 2258
BstUI CGCG 3 cut(s) 605, 1878, 1907
BstV1I GCAGC 8 cut(s) 214, 557, 566, 1140, 1547, 1618, 1638, 2165
BstX2I RGATCY 2 cut(s) 2278, 2296
BstYI RGATCY 2 cut(s) 2278, 2296
Bsu36I CCTNAGG 1 cut(s) 738
BsuRI GGCC 8 cut(s) 119, 135, 378, 1216, 1325, 1338, 1418, 1589
BtgI CCRYGG 1 cut(s) 57
BtgZI GCGATG 2 cut(s) 586, 1354
BtrI CACGTC 1 cut(s) 1621
BtsCI GGATG 5 cut(s) 78, 1271, 1549, 1876, 1912
BtsI GCAGTG 1 cut(s) 1551
BtsIMutI CAGTG 4 cut(s) 1551, 1605, 1938, 2154
BtuMI TCGCGA 1 cut(s) 1907
BveI ACCTGC 2 cut(s) 584, 2116
Cac8I GCNNGC 7 cut(s) 65, 76, 829, 1323, 1647, 1791, 1950
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 4 cut(s) 795, 1133, 1631, 1801
Cfr10I RCCGGY 1 cut(s) 827
Cfr13I GGNCC 6 cut(s) 134, 170, 1214, 1337, 1417, 1865
CpoI CGGWCCG 1 cut(s) 1865
CspCI CAANNNNNGTGG 2 cut(s) 1003, 1038
CspI CGGWCCG 1 cut(s) 1865
DdeI CTNAG 9 cut(s) 144, 358, 499, 728, 738, 1007, 1557, 2159, 2237
DpnI GATC 8 cut(s) 599, 644, 782, 1302, 1427, 1904, 2280, 2298
DpnII GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
Eam1104I CTCTTC 5 cut(s) 275, 1259, 1494, 1859, 2244
EarI CTCTTC 5 cut(s) 275, 1259, 1494, 1859, 2244
EciI GGCGGA 2 cut(s) 1524, 1878
Eco130I CCWWGG 6 cut(s) 57, 120, 649, 843, 1078, 1276
Eco147I AGGCCT 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 1388
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 170, 1865
Eco47III AGCGCT 1 cut(s) 1630
Eco57I CTGAAG 1 cut(s) 2277
Eco81I CCTNAGG 1 cut(s) 738
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 206, 2341
EcoRII CCWGG 1 cut(s) 130
EcoRV GATATC 1 cut(s) 1114
EcoT14I CCWWGG 6 cut(s) 57, 120, 649, 843, 1078, 1276
EcoT38I GRGCYC 1 cut(s) 1388
ErhI CCWWGG 6 cut(s) 57, 120, 649, 843, 1078, 1276
Esp3I CGTCTC 1 cut(s) 1597
FaqI GGGAC 4 cut(s) 138, 644, 1116, 2186
FauI CCCGC 1 cut(s) 1374
FbaI TGATCA 2 cut(s) 597, 780
FokI GGATG 5 cut(s) 65, 1258, 1556, 1883, 1899
FriOI GRGCYC 1 cut(s) 1388
FspAI RTGCGCAY 1 cut(s) 794
FspBI CTAG 5 cut(s) 438, 650, 1145, 1802, 1917
FspI TGCGCA 1 cut(s) 794
GlaI GCGC 4 cut(s) 794, 1132, 1630, 1800
GsaI CCCAGC 1 cut(s) 1797
GsuI CTGGAG 2 cut(s) 985, 1493
HaeII RGCGCY 3 cut(s) 1134, 1632, 1802
HaeIII GGCC 8 cut(s) 119, 135, 378, 1216, 1325, 1338, 1418, 1589
HapII CCGG 5 cut(s) 262, 828, 1298, 1868, 2043
HhaI GCGC 4 cut(s) 795, 1133, 1631, 1801
Hin6I GCGC 4 cut(s) 793, 1131, 1629, 1799
HinP1I GCGC 4 cut(s) 793, 1131, 1629, 1799
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 219
HpaII CCGG 5 cut(s) 262, 828, 1298, 1868, 2043
HphI GGTGA 2 cut(s) 474, 2140
Hpy166II GTNNAC 9 cut(s) 43, 474, 895, 916, 1333, 1821, 1828, 2131, 2207
Hpy188I TCNGA 8 cut(s) 147, 196, 529, 731, 1305, 1440, 1758, 2296
Hpy188III TCNNGA 5 cut(s) 640, 1090, 1466, 1594, 1906
Hpy8I GTNNAC 9 cut(s) 43, 474, 895, 916, 1333, 1821, 1828, 2131, 2207
Hpy99I CGWCG 5 cut(s) 569, 1165, 1496, 1694, 1963
HpyAV CCTTC 6 cut(s) 400, 842, 1093, 1558, 1972, 2194
HpyCH4III ACNGT 7 cut(s) 350, 1204, 1600, 1825, 1933, 2149, 2272
HpyCH4IV ACGT 5 cut(s) 706, 797, 1455, 1620, 1830
HpyF3I CTNAG 9 cut(s) 144, 358, 499, 728, 738, 1007, 1557, 2159, 2237
HpySE526I ACGT 5 cut(s) 706, 797, 1455, 1620, 1830
HspAI GCGC 4 cut(s) 793, 1131, 1629, 1799
KroI GCCGGC 1 cut(s) 827
KroNI GCCGGC 1 cut(s) 829
Ksp22I TGATCA 2 cut(s) 597, 780
Kzo9I GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
LmnI GCTCC 4 cut(s) 503, 1391, 1759, 2138
Lsp1109I GCAGC 8 cut(s) 214, 557, 566, 1140, 1547, 1618, 1638, 2165
LweI GCATC 7 cut(s) 87, 462, 557, 934, 1534, 1687, 2105
MaeI CTAG 5 cut(s) 438, 650, 1145, 1802, 1917
MaeII ACGT 5 cut(s) 706, 797, 1455, 1620, 1830
MaeIII GTNAC 4 cut(s) 459, 616, 1147, 1176
MalI GATC 8 cut(s) 599, 644, 782, 1302, 1427, 1904, 2280, 2298
MboI GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
MfeI CAATTG 1 cut(s) 1884
MflI RGATCY 2 cut(s) 2278, 2296
MhlI GDGCHC 3 cut(s) 1388, 1524, 2258
MlyI GAGTC 3 cut(s) 958, 1245, 1555
MroNI GCCGGC 1 cut(s) 827
MseI TTAA 7 cut(s) 410, 512, 801, 1197, 1232, 1923, 2301
MslI CAYNNNNRTG 4 cut(s) 62, 788, 992, 1454
MspA1I CMGCKG 1 cut(s) 1538
MspI CCGG 5 cut(s) 262, 828, 1298, 1868, 2043
MspR9I CCNGG 2 cut(s) 132, 2044
MunI CAATTG 1 cut(s) 1884
MvaI CCWGG 1 cut(s) 132
MvnI CGCG 3 cut(s) 605, 1878, 1907
NaeI GCCGGC 1 cut(s) 829
NciI CCSGG 1 cut(s) 2044
NcoI CCATGG 1 cut(s) 57
NdeII GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
NgoMIV GCCGGC 1 cut(s) 827
NlaIV GGNNCC 6 cut(s) 136, 171, 355, 660, 1157, 1387
NmeAIII GCCGAG 1 cut(s) 1660
NruI TCGCGA 1 cut(s) 1907
NsbI TGCGCA 1 cut(s) 794
NspI RCATGY 4 cut(s) 67, 1109, 1351, 1844
OliI CACNNNNGTG 1 cut(s) 1454
PaeI GCATGC 1 cut(s) 67
PaqCI CACCTGC 1 cut(s) 2116
PceI AGGCCT 1 cut(s) 119
PciI ACATGT 2 cut(s) 1347, 1840
PcsI WCGNNNNNNNCGW 6 cut(s) 441, 1488, 1512, 1521, 1601, 1964
PdiI GCCGGC 1 cut(s) 829
PfeI GAWTC 8 cut(s) 148, 265, 1054, 1566, 1686, 1703, 1909, 1955
PflFI GACNNNGTC 1 cut(s) 1600
PleI GAGTC 3 cut(s) 957, 1245, 1555
PpsI GAGTC 3 cut(s) 957, 1245, 1555
PscI ACATGT 2 cut(s) 1347, 1840
PshAI GACNNNNGTC 1 cut(s) 534
PshBI ATTAAT 1 cut(s) 1232
PsiI TTATAA 1 cut(s) 2082
Psp6I CCWGG 1 cut(s) 130
PspFI CCCAGC 1 cut(s) 1793
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 6 cut(s) 136, 171, 355, 660, 1157, 1387
PspPI GGNCC 6 cut(s) 134, 170, 1214, 1337, 1417, 1865
PstI CTGCAG 2 cut(s) 232, 556
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 2 cut(s) 2278, 2296
PsyI GACNNNGTC 1 cut(s) 1600
PvuII CAGCTG 1 cut(s) 1538
RruI TCGCGA 1 cut(s) 1907
RseI CAYNNNNRTG 4 cut(s) 62, 788, 992, 1454
Rsr2I CGGWCCG 1 cut(s) 1865
RsrII CGGWCCG 1 cut(s) 1865
SaqAI TTAA 7 cut(s) 410, 512, 801, 1197, 1232, 1923, 2301
Sau3AI GATC 8 cut(s) 597, 642, 780, 1300, 1425, 1902, 2278, 2296
Sau96I GGNCC 6 cut(s) 134, 170, 1214, 1337, 1417, 1865
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 3 cut(s) 958, 1245, 1555
ScrFI CCNGG 2 cut(s) 132, 2044
SduI GDGCHC 3 cut(s) 1388, 1524, 2258
SfaNI GCATC 7 cut(s) 87, 462, 557, 934, 1534, 1687, 2105
SfcI CTRYAG 2 cut(s) 228, 552
SinI GGWCC 2 cut(s) 170, 1865
SmiMI CAYNNNNRTG 4 cut(s) 62, 788, 992, 1454
SmlI CTYRAG 2 cut(s) 113, 1090
SmoI CTYRAG 2 cut(s) 113, 1090
SpeI ACTAGT 1 cut(s) 1144
SphI GCATGC 1 cut(s) 67
SseBI AGGCCT 1 cut(s) 119
SsiI CCGC 9 cut(s) 366, 1034, 1381, 1447, 1509, 1718, 1723, 1863, 1876
SspI AATATT 1 cut(s) 190
SspMI CTAG 5 cut(s) 438, 650, 1145, 1802, 1917
StuI AGGCCT 1 cut(s) 119
StyD4I CCNGG 2 cut(s) 130, 2042
StyI CCWWGG 6 cut(s) 57, 120, 649, 843, 1078, 1276
TaaI ACNGT 7 cut(s) 350, 1204, 1600, 1825, 1933, 2149, 2272
TaiI ACGT 5 cut(s) 709, 800, 1458, 1623, 1833
TaqII GACCGA 1 cut(s) 1354
TatI WGTACW 5 cut(s) 681, 904, 973, 1895, 2178
TauI GCSGC 2 cut(s) 369, 1449
TfiI GAWTC 8 cut(s) 148, 265, 1054, 1566, 1686, 1703, 1909, 1955
Tru1I TTAA 7 cut(s) 410, 512, 801, 1197, 1232, 1923, 2301
Tru9I TTAA 7 cut(s) 410, 512, 801, 1197, 1232, 1923, 2301
TscAI CASTG 4 cut(s) 1558, 1605, 1938, 2154
TseI GCWGC 8 cut(s) 227, 545, 554, 1128, 1535, 1626, 1631, 2153
TspDTI ATGAA 6 cut(s) 262, 976, 1250, 1991, 2004, 2008
TspGWI ACGGA 2 cut(s) 162, 1753
TspRI CASTG 4 cut(s) 1558, 1605, 1938, 2154
Tth111I GACNNNGTC 1 cut(s) 1600
VpaK11BI GGWCC 2 cut(s) 170, 1865
VspI ATTAAT 1 cut(s) 1232
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 5 cut(s) 17, 206, 1606, 1740, 2341
XceI RCATGY 4 cut(s) 67, 1109, 1351, 1844
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XmaJI CCTAGG 1 cut(s) 649
XspI CTAG 5 cut(s) 438, 650, 1145, 1802, 1917
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.