Prupe.5G050500_v2.0.a1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0014153
Physical Location & Seq
Forward (+)
5379728 .. 5383056
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G050500.1

Sequence Viewer

Length: 2373 bp
ATGGAGGCTACTCACAAATTCGTGTGCCAAGACGATTTGGTCAACCCACTTTCCTCCATGGGAATGCAAGTAGCTTGCATCCTTGTCATCTCACACTTCTTTCACATTTTGCTCAAGGCCTTAGGTCAACCAGGCCCCATTGCCCAGATTCTTGCTGGTTTGGTGTTGGGTCCTTCAGGGTTGTCTAATATTACAGAAATAAAAGAATTCTTCTTTCAAGCTTCTGCTGCAGAATACTATGAGATGTTTGGGTTCTTCTGCCGGATCCTCTTTATGTTCCTCTTCGGTTTGGAGATGGACTTTGCCTATGTACGCCGCAACCTGCGCGTAGTGTGCATCGTAGCCTACGGTGGTGCCTTCTTAGCCAGCCTTTTCGGCCTTACCGCCTCCTTCTTTATCTACAAACACTTACTGGCTGAGAATGCCAAGCTGAATGCTAGCTTTGTCTTCTGCACCATGCTATTGGTGTCCTACACCTCCTCACCAGTCGTCAACCGCTTAGCAGCAGAGTTAAGGTTTGCAACATCAGACATTGGCCGCTTGGCAACATCTTCTGCCATGGTCGTTGAGCTCACTTGCTTACTTGTTTTTAATCTGATCATTGCCTTCACAAGACTAACTGCCTTGAGGGACGGCTTCTTCATCCTCCTCGGCTTGGTTCCCATGCTTTTCGCATTCAAGTACTTGGCAGTGTGGTTGAACAAACGAAACAGAAAGCAGAAGTACCTTAGAAACCCAGAAGTGTTTCTCATTTTGTCGATTCTAATCGCGGGTTCGATGCTCATTGAAATGGCCATGTTTAATAGTGTGATAGCTTGTTTTGTTGCTGGCCTGGCTTTCCCCAAGGAAGGCAAGACGGCAAGAACTTTGTTGCACAAGCTTACTTACTCTGTCCACAACTTTGTGTTGCCTGTTTACTTTGGCTATATTGGGTTCCAATTTGATGCGAGCCATTTGAAGAGCTTGACCAACATATTGATTATTGCCATACTTGTGTTGTTGAGCCTCGGCAGCAAGATTAGTGGCACTCTTGCGGTTTGCCACTTTTTGAAGATCCCTTTAAACGAAGGGGTTTTTCTTGGCTTTGTGTTGAACTTGAAGGGACATGCCGATCTCTTGTTCGTTGGCAGCGCCTCGAAGGCTCTAATTACTTGGAACCCAGCGGCTTACAATCTGTTACTAATAACAGTTGTGATTAACACTGTAATCTCAGGGCCAGTTGTGGCTTTGTTAATAAGAAGAGAAGAAAAACTCTTCACTCACACACACACCTCCCTCGGCTTTGAGCCCGAAAATCCCGACCAAAACGAGCTCCGCCTCCTCGCCTGCGTCTACGGTCCCCGCCACATCTCTGCCATCCTCTCCGTCATCGCCACCATCCGCGGCACCCAAACAGCATCCATCATGCCCTACATGGCTCACCTTATCGAGCTCAACCAAAAACGACGCACCAACGTGTCGTATCACGAGCTCGAAGCCGAGGAAATGAGCGACGAGGAAGACTACGGCGGCAACGACGTCCTCGAAATCCATGCAGCCGTCGACGCCTTCACTGCCGAGACCCAGATCCTAATCAACCTCAACAAGGCCGTGTCCACGTTGTCGAATTTACATGAGGAGGTGTGTAACGCAGCCGAGGACTTGCGTGCGACGATTATTTTGCTGCCGTTTCACAAGCACCAAAGGATCGACGGCAAAATGGAGTCCGGGAAGGACGCTGTACGGACCACGAATCAAAAGATTCTTCGTCACGCCCCGAGCTCTGTCGGGATGATCGTCGAGAAAGGGCTGGCCGGGGCGTTAGGGTTTTCGCAGTTGTTTACCGTGGACATTATGCAACATGTTGCGACGCTATTTTTCGGGGGGCCCGATGACCGCGAGGCCATTGCTTGGAGTACAAGGATCGCAAACCATCCAAGGGTTAACTTGACGGTGGTTAGGTTTTTGGCGACGGAGTCATCCAACACTCGGAATATGAAGGTGGAGAATGAAACGGGAGGCAATAGTGATATTGAGGTTTACATGGCCTTGTCGAGTTTAGAAACGGGCAATGACATTGACAATGCCTTTCTCAATGACTTCTATAATGGGTATGTGGCGTCTGGTAAAGTTGGGTACGTAGAGAAGCAGGTAAATAATGGAGCAGAAACAGTGGCAGCCCTAAGAGACATTGGAGACTTGTACTCTCTGTTTATAGTAGGGAGGGGTGGTCGAGGACACTCACCATTGACAACCGAGCTCAGTGATTGGGAAGAGTGCCCTGAACTTGGAACAGTAGGAGATCTCTTGGCTTCTTCAGACTTCAATATCAATGGCTCAATCTTGGTCGTCCAACAACATAGAAATTCCAAGAAAGAACTTGTAGATGATTAG

Protein Analysis

791

Amino Acids

87.16

Weight (kDa)

6.1

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16380 AT1G79400
fragaria_vesca FvH4_5g20830 FvH4_5g20830
malus_domestica MD06G1082400.v1.1
prunus_persica Prupe.5G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0207831
rosa_laevigata RLG00000003284
rosa_multiflora Rmu_sc0004647.1_g000005
rosa_roxburghii Rroxscaffold_3G00251120
rosa_rugosa Rorug07G0099700 Rorug07G0099800
rosa_samantha Rh7AG233000 Rh7BG228300 Rh7CG248000 Rh7DG239000
rosa_wichuraiana Rw7G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AatII GACGTC 1 cut(s) 1521
Acc36I ACCTGC 2 cut(s) 330, 2119
AccB1I GGYRCC 2 cut(s) 353, 1385
AccB7I CCANNNNNTGG 1 cut(s) 1890
AccI GTMKAC 2 cut(s) 1332, 1542
AccII CGCG 4 cut(s) 327, 770, 1383, 1878
AclWI GGATC 6 cut(s) 259, 272, 1048, 1561, 1694, 1910
AcoI YGGCCR 3 cut(s) 535, 792, 1791
AcsI RAATTY 4 cut(s) 17, 206, 1606, 2344
AcuI CTGAAG 2 cut(s) 159, 2280
AcyI GRCGYC 3 cut(s) 1518, 1545, 2099
AfaI GTAC 7 cut(s) 312, 683, 725, 1722, 1897, 2117, 2183
AfiI CCNNNNNNNGG 7 cut(s) 655, 848, 1585, 1890, 1918, 1968, 2267
AflIII ACRYGT 2 cut(s) 1455, 1840
AgsI TTSAA 9 cut(s) 218, 679, 700, 788, 958, 1051, 1093, 1099, 2305
AjnI CCWGG 2 cut(s) 130, 831
AleI CACNNNNGTG 1 cut(s) 1454
Alw21I GWGCWC 6 cut(s) 573, 1314, 1434, 1473, 1763, 2241
Alw26I GTCTC 3 cut(s) 1553, 2160, 2169
AlwI GGATC 6 cut(s) 259, 272, 1048, 1561, 1694, 1910
Ama87I CYCGRG 1 cut(s) 1756
ApaI GGGCCC 1 cut(s) 1869
ApeKI GCWGC 8 cut(s) 227, 503, 1011, 1128, 1535, 1631, 1663, 2156
ApoI RAATTY 4 cut(s) 17, 206, 1606, 2344
ArsI GACNNNNNNTTYG 2 cut(s) 1642, 1674
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 2 cut(s) 327, 1133
AspS9I GGNCC 7 cut(s) 134, 170, 1214, 1337, 1725, 1865, 1866
AsuC2I CCSGG 2 cut(s) 1708, 1795
AsuHPI GGTGA 3 cut(s) 474, 1412, 2214
AsuNHI GCTAGC 1 cut(s) 437
AvaI CYCGRG 1 cut(s) 1756
AvaII GGWCC 3 cut(s) 170, 1337, 1725
AxyI CCTNAGG 1 cut(s) 121
BaeGI GKGCMC 2 cut(s) 1869, 2261
BalI TGGCCA 1 cut(s) 794
BamHI GGATCC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 353, 1385
BanII GRGCYC 8 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241
BauI CACGAG 1 cut(s) 1466
BbsI GAAGAC 2 cut(s) 439, 1506
Bbv12I GWGCWC 6 cut(s) 573, 1314, 1434, 1473, 1763, 2241
BbvI GCAGC 8 cut(s) 214, 515, 1023, 1140, 1547, 1643, 1650, 2168
BccI CCATC 5 cut(s) 289, 1364, 1385, 1409, 1920
BceAI ACGGC 7 cut(s) 649, 873, 1522, 1523, 1574, 1651, 1708
BcgI CGANNNNNNTGC 6 cut(s) 1514, 1548, 1642, 1676, 1868, 1902
BciT130I CCWGG 2 cut(s) 132, 833
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 2 cut(s) 1708, 1795
BcoDI GTCTC 3 cut(s) 1553, 2160, 2169
BfaI CTAG 1 cut(s) 438
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 1 cut(s) 1134
BfuAI ACCTGC 2 cut(s) 330, 2119
BglI GCCNNNNNGGC 2 cut(s) 375, 1139
BglII AGATCT 1 cut(s) 2281
BlpI GCTNAGC 1 cut(s) 499
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 4 cut(s) 132, 833, 1708, 1795
Bme18I GGWCC 3 cut(s) 170, 1337, 1725
BmeT110I CYCGRG 1 cut(s) 1756
BmgT120I GGNCC 7 cut(s) 134, 170, 1214, 1337, 1725, 1865, 1866
BmrFI CCNGG 4 cut(s) 132, 833, 1708, 1795
BmsI GCATC 5 cut(s) 87, 345, 768, 934, 1406
BmtI GCTAGC 1 cut(s) 441
BpiI GAAGAC 2 cut(s) 439, 1506
Bpu1102I GCTNAGC 1 cut(s) 499
BpuEI CTTGAG 2 cut(s) 98, 646
BpuMI CCSGG 2 cut(s) 1708, 1795
BsaAI YACGTR 1 cut(s) 2119
BsaBI GATNNNNATC 2 cut(s) 764, 1571
BsaHI GRCGYC 3 cut(s) 1518, 1545, 2099
BsaI GGTCTC 1 cut(s) 1553
BsaXI ACNNNNNCTCC 2 cut(s) 2194, 2224
Bsc4I CCNNNNNNNGG 7 cut(s) 655, 848, 1585, 1890, 1918, 1968, 2267
Bse1I ACTGG 3 cut(s) 417, 485, 1217
Bse21I CCTNAGG 1 cut(s) 121
Bse3DI GCAATG 4 cut(s) 138, 600, 1884, 2056
Bse8I GATNNNNATC 2 cut(s) 764, 1571
BseBI CCWGG 2 cut(s) 132, 833
BseGI GGATG 8 cut(s) 78, 642, 1356, 1377, 1397, 1776, 1912, 1958
BseJI GATNNNNATC 2 cut(s) 764, 1571
BseLI CCNNNNNNNGG 7 cut(s) 655, 848, 1585, 1890, 1918, 1968, 2267
BseMI GCAATG 4 cut(s) 138, 600, 1884, 2056
BseMII CTCAG 3 cut(s) 408, 1224, 2254
BseNI ACTGG 3 cut(s) 417, 485, 1217
BseRI GAGGAG 4 cut(s) 469, 638, 1310, 1631
BseSI GKGCMC 2 cut(s) 1869, 2261
BseXI GCAGC 8 cut(s) 214, 515, 1023, 1140, 1547, 1643, 1650, 2168
BseYI CCCAGC 1 cut(s) 1159
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 4 cut(s) 327, 770, 1383, 1878
BshNI GGYRCC 2 cut(s) 353, 1385
BsiHKAI GWGCWC 6 cut(s) 573, 1314, 1434, 1473, 1763, 2241
BsiHKCI CYCGRG 1 cut(s) 1756
BsiSI CCGG 3 cut(s) 262, 1707, 1794
BslFI GGGAC 3 cut(s) 644, 1116, 1323
BslI CCNNNNNNNGG 7 cut(s) 655, 848, 1585, 1890, 1918, 1968, 2267
BsmAI GTCTC 3 cut(s) 1553, 2160, 2169
BsmFI GGGAC 3 cut(s) 644, 1116, 1323
BsmI GAATGC 4 cut(s) 69, 427, 439, 674
Bso31I GGTCTC 1 cut(s) 1553
BsoBI CYCGRG 1 cut(s) 1756
Bsp120I GGGCCC 1 cut(s) 1865
Bsp1286I GDGCHC 9 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241, 2261
Bsp143I GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
Bsp1720I GCTNAGC 1 cut(s) 499
Bsp19I CCATGG 2 cut(s) 57, 558
BspCNI CTCAG 3 cut(s) 409, 1223, 2253
BspFNI CGCG 4 cut(s) 327, 770, 1383, 1878
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 2 cut(s) 330, 2119
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 6 cut(s) 259, 272, 1048, 1561, 1694, 1910
BspQI GCTCTTC 1 cut(s) 953
BspT107I GGYRCC 2 cut(s) 353, 1385
BspTNI GGTCTC 1 cut(s) 1553
BsrDI GCAATG 4 cut(s) 138, 600, 1884, 2056
BsrI ACTGG 3 cut(s) 417, 485, 1217
BssMI GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
BssNI GRCGYC 3 cut(s) 1518, 1545, 2099
BssSI CACGAG 1 cut(s) 1466
BssT1I CCWWGG 4 cut(s) 57, 558, 843, 1916
Bst2BI CACGAG 1 cut(s) 1466
Bst2UI CCWGG 2 cut(s) 132, 833
Bst4CI ACNGT 8 cut(s) 350, 1189, 1204, 1337, 1825, 1933, 2152, 2275
Bst6I CTCTTC 5 cut(s) 287, 953, 1234, 1259, 2247
BstACI GRCGYC 3 cut(s) 1518, 1545, 2099
BstBAI YACGTR 1 cut(s) 2119
BstC8I GCNNGC 8 cut(s) 76, 367, 439, 829, 949, 1327, 1647, 1791
BstDEI CTNAG 8 cut(s) 121, 361, 417, 499, 728, 1210, 2162, 2240
BstDSI CCRYGG 4 cut(s) 57, 558, 1381, 1824
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 8 cut(s) 78, 642, 1356, 1377, 1397, 1776, 1912, 1958
BstFNI CGCG 4 cut(s) 327, 770, 1383, 1878
BstH2I RGCGCY 1 cut(s) 1134
BstHHI GCGC 2 cut(s) 327, 1133
BstKTI GATC 9 cut(s) 267, 600, 1056, 1114, 1569, 1689, 1776, 1905, 2284
BstMAI GTCTC 3 cut(s) 1553, 2160, 2169
BstMBI GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
BstNI CCWGG 2 cut(s) 132, 833
BstNSI RCATGY 2 cut(s) 1109, 1844
BstSCI CCNGG 4 cut(s) 130, 831, 1706, 1793
BstSFI CTRYAG 1 cut(s) 228
BstSLI GKGCMC 2 cut(s) 1869, 2261
BstSNI TACGTA 1 cut(s) 2119
BstUI CGCG 4 cut(s) 327, 770, 1383, 1878
BstV1I GCAGC 8 cut(s) 214, 515, 1023, 1140, 1547, 1643, 1650, 2168
BstV2I GAAGAC 2 cut(s) 439, 1506
BstX2I RGATCY 4 cut(s) 264, 1053, 1566, 2281
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 4 cut(s) 264, 1053, 1566, 2281
Bsu36I CCTNAGG 1 cut(s) 121
BtgI CCRYGG 4 cut(s) 57, 558, 1381, 1824
BtgZI GCGATG 1 cut(s) 1354
BtsCI GGATG 8 cut(s) 78, 642, 1356, 1377, 1397, 1776, 1912, 1958
BtsI GCAGTG 2 cut(s) 696, 1551
BtsIMutI CAGTG 5 cut(s) 696, 1200, 1551, 2157, 2248
BveI ACCTGC 2 cut(s) 330, 2119
Cac8I GCNNGC 8 cut(s) 76, 367, 439, 829, 949, 1327, 1647, 1791
CfoI GCGC 2 cut(s) 327, 1133
Cfr13I GGNCC 7 cut(s) 134, 170, 1214, 1337, 1725, 1865, 1866
Cfr42I CCGCGG 1 cut(s) 1384
CseI GACGC 6 cut(s) 1318, 1455, 1553, 1724, 1858, 2088
Csp6I GTAC 7 cut(s) 311, 682, 724, 1721, 1896, 2116, 2182
CspCI CAANNNNNGTGG 2 cut(s) 1003, 1038
CviQI GTAC 7 cut(s) 311, 682, 724, 1721, 1896, 2116, 2182
DdeI CTNAG 8 cut(s) 121, 361, 417, 499, 728, 1210, 2162, 2240
DpnI GATC 9 cut(s) 266, 599, 1055, 1113, 1568, 1688, 1775, 1904, 2283
DpnII GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
DraI TTTAAA 1 cut(s) 1062
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 3 cut(s) 535, 792, 1791
Eam1104I CTCTTC 5 cut(s) 287, 953, 1234, 1259, 2247
EarI CTCTTC 5 cut(s) 287, 953, 1234, 1259, 2247
EciI GGCGGA 1 cut(s) 1304
Ecl136II GAGCTC 6 cut(s) 571, 1312, 1432, 1471, 1761, 2239
Eco105I TACGTA 1 cut(s) 2119
Eco130I CCWWGG 4 cut(s) 57, 558, 843, 1916
Eco147I AGGCCT 1 cut(s) 119
Eco24I GRGCYC 8 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241
Eco31I GGTCTC 1 cut(s) 1553
Eco47I GGWCC 3 cut(s) 170, 1337, 1725
Eco53kI GAGCTC 6 cut(s) 571, 1312, 1432, 1471, 1761, 2239
Eco57I CTGAAG 2 cut(s) 159, 2280
Eco81I CCTNAGG 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 1756
EcoICRI GAGCTC 6 cut(s) 571, 1312, 1432, 1471, 1761, 2239
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoO109I RGGNCCY 3 cut(s) 134, 170, 1865
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 130, 831
EcoT14I CCWWGG 4 cut(s) 57, 558, 843, 1916
EcoT38I GRGCYC 8 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241
ErhI CCWWGG 4 cut(s) 57, 558, 843, 1916
FaqI GGGAC 3 cut(s) 644, 1116, 1323
FauI CCCGC 2 cut(s) 763, 1349
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 2 cut(s) 1332, 1542
FokI GGATG 8 cut(s) 65, 629, 1343, 1364, 1384, 1783, 1899, 1945
FriOI GRGCYC 8 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241
FspBI CTAG 1 cut(s) 438
GlaI GCGC 2 cut(s) 326, 1132
GsaI CCCAGC 1 cut(s) 1163
HaeII RGCGCY 1 cut(s) 1134
HapII CCGG 3 cut(s) 262, 1707, 1794
HgaI GACGC 6 cut(s) 1318, 1455, 1553, 1724, 1858, 2088
HhaI GCGC 2 cut(s) 327, 1133
Hin1I GRCGYC 3 cut(s) 1518, 1545, 2099
Hin6I GCGC 2 cut(s) 325, 1131
HinP1I GCGC 2 cut(s) 325, 1131
HincII GTYRAC 5 cut(s) 43, 128, 493, 1543, 1924
HindII GTYRAC 5 cut(s) 43, 128, 493, 1543, 1924
HindIII AAGCTT 2 cut(s) 219, 878
HinfI GANTC 6 cut(s) 148, 760, 1703, 1732, 1741, 1955
HpaI GTTAAC 1 cut(s) 1924
HpaII CCGG 3 cut(s) 262, 1707, 1794
HphI GGTGA 3 cut(s) 474, 1412, 2214
Hpy188I TCNGA 4 cut(s) 529, 597, 1971, 2299
Hpy188III TCNNGA 4 cut(s) 1298, 1466, 1768, 1780
HpyCH4III ACNGT 8 cut(s) 350, 1189, 1204, 1337, 1825, 1933, 2152, 2275
HpyCH4IV ACGT 4 cut(s) 1455, 1518, 1598, 2118
HpyCH4V TGCA 9 cut(s) 67, 78, 230, 336, 453, 521, 874, 1535, 1837
HpyF3I CTNAG 8 cut(s) 121, 361, 417, 499, 728, 1210, 2162, 2240
HpySE526I ACGT 4 cut(s) 1455, 1518, 1598, 2118
Hsp92I GRCGYC 3 cut(s) 1518, 1545, 2099
HspAI GCGC 2 cut(s) 325, 1131
Ksp22I TGATCA 1 cut(s) 597
KspAI GTTAAC 1 cut(s) 1924
KspI CCGCGG 1 cut(s) 1384
Kzo9I GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
LguI GCTCTTC 1 cut(s) 953
LmnI GCTCC 2 cut(s) 1317, 2141
Lsp1109I GCAGC 8 cut(s) 214, 515, 1023, 1140, 1547, 1643, 1650, 2168
LweI GCATC 5 cut(s) 87, 345, 768, 934, 1406
MaeI CTAG 1 cut(s) 438
MaeII ACGT 4 cut(s) 1455, 1518, 1598, 2118
MaeIII GTNAC 3 cut(s) 1176, 1625, 1748
MalI GATC 9 cut(s) 266, 599, 1055, 1113, 1568, 1688, 1775, 1904, 2283
MboI GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
MflI RGATCY 4 cut(s) 264, 1053, 1566, 2281
MhlI GDGCHC 9 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241, 2261
MlsI TGGCCA 1 cut(s) 794
MluCI AATT 6 cut(s) 17, 206, 938, 1146, 1606, 2344
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 2 cut(s) 1712, 1964
MmeI TCCRAC 2 cut(s) 1986, 2356
Mox20I TGGCCA 1 cut(s) 794
MroXI GAANNNNTTC 1 cut(s) 744
MscI TGGCCA 1 cut(s) 794
MseI TTAA 7 cut(s) 512, 591, 801, 1061, 1197, 1232, 1923
MslI CAYNNNNRTG 4 cut(s) 62, 788, 992, 1454
Msp20I TGGCCA 1 cut(s) 794
MspA1I CMGCKG 2 cut(s) 1163, 1383
MspI CCGG 3 cut(s) 262, 1707, 1794
MspR9I CCNGG 4 cut(s) 132, 833, 1708, 1795
Mva1269I GAATGC 4 cut(s) 69, 427, 439, 674
MvaI CCWGG 2 cut(s) 132, 833
MvnI CGCG 4 cut(s) 327, 770, 1383, 1878
NciI CCSGG 2 cut(s) 1708, 1795
NcoI CCATGG 2 cut(s) 57, 558
NdeII GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
NheI GCTAGC 1 cut(s) 437
NmeAIII GCCGAG 6 cut(s) 630, 987, 1257, 1504, 1582, 1660
NmuCI GTSAC 1 cut(s) 1748
NspI RCATGY 2 cut(s) 1109, 1844
OliI CACNNNNGTG 1 cut(s) 1454
PceI AGGCCT 1 cut(s) 119
PciI ACATGT 1 cut(s) 1840
PciSI GCTCTTC 1 cut(s) 953
PcsI WCGNNNNNNNCGW 4 cut(s) 1512, 1521, 1773, 1866
PctI GAATGC 4 cut(s) 69, 427, 439, 674
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 4 cut(s) 148, 760, 1732, 1741
PflFI GACNNNGTC 1 cut(s) 1954
PflMI CCANNNNNTGG 1 cut(s) 1890
PfoI TCCNGGA 1 cut(s) 1706
PleI GAGTC 2 cut(s) 1711, 1963
PpsI GAGTC 2 cut(s) 1711, 1963
Ppu21I YACGTR 1 cut(s) 2119
PpuMI RGGWCCY 1 cut(s) 170
PscI ACATGT 1 cut(s) 1840
Psp124BI GAGCTC 6 cut(s) 573, 1314, 1434, 1473, 1763, 2241
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 130, 831
PspFI CCCAGC 1 cut(s) 1159
PspGI CCWGG 2 cut(s) 130, 831
PspOMI GGGCCC 1 cut(s) 1865
PspPI GGNCC 7 cut(s) 134, 170, 1214, 1337, 1725, 1865, 1866
PspPPI RGGWCCY 1 cut(s) 170
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 4 cut(s) 264, 1053, 1566, 2281
PsyI GACNNNGTC 1 cut(s) 1954
RsaI GTAC 7 cut(s) 312, 683, 725, 1722, 1897, 2117, 2183
RsaNI GTAC 7 cut(s) 311, 682, 724, 1721, 1896, 2116, 2182
RseI CAYNNNNRTG 4 cut(s) 62, 788, 992, 1454
SacI GAGCTC 6 cut(s) 573, 1314, 1434, 1473, 1763, 2241
SacII CCGCGG 1 cut(s) 1384
SalI GTCGAC 1 cut(s) 1541
SapI GCTCTTC 1 cut(s) 953
SaqAI TTAA 7 cut(s) 512, 591, 801, 1061, 1197, 1232, 1923
Sau3AI GATC 9 cut(s) 264, 597, 1053, 1111, 1566, 1686, 1773, 1902, 2281
Sau96I GGNCC 7 cut(s) 134, 170, 1214, 1337, 1725, 1865, 1866
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 2 cut(s) 1712, 1964
ScrFI CCNGG 4 cut(s) 132, 833, 1708, 1795
SduI GDGCHC 9 cut(s) 573, 1290, 1314, 1434, 1473, 1763, 1869, 2241, 2261
SfaNI GCATC 5 cut(s) 87, 345, 768, 934, 1406
SfcI CTRYAG 1 cut(s) 228
Sfr303I CCGCGG 1 cut(s) 1384
SgrBI CCGCGG 1 cut(s) 1384
SgrDI CGTCGACG 1 cut(s) 1541
SinI GGWCC 3 cut(s) 170, 1337, 1725
SmiMI CAYNNNNRTG 4 cut(s) 62, 788, 992, 1454
SmlI CTYRAG 2 cut(s) 113, 625
SmoI CTYRAG 2 cut(s) 113, 625
SnaBI TACGTA 1 cut(s) 2119
Sse9I AATT 6 cut(s) 17, 206, 938, 1146, 1606, 2344
SseBI AGGCCT 1 cut(s) 119
SspI AATATT 1 cut(s) 190
SspMI CTAG 1 cut(s) 438
SstI GAGCTC 6 cut(s) 573, 1314, 1434, 1473, 1763, 2241
StuI AGGCCT 1 cut(s) 119
StyD4I CCNGG 4 cut(s) 130, 831, 1706, 1793
StyI CCWWGG 4 cut(s) 57, 558, 843, 1916
TaaI ACNGT 8 cut(s) 350, 1189, 1204, 1337, 1825, 1933, 2152, 2275
TaiI ACGT 4 cut(s) 1458, 1521, 1601, 2121
TasI AATT 6 cut(s) 17, 206, 938, 1146, 1606, 2344
TatI WGTACW 3 cut(s) 681, 1895, 2181
TauI GCSGC 5 cut(s) 318, 540, 1166, 1386, 1512
TfiI GAWTC 4 cut(s) 148, 760, 1732, 1741
Tru1I TTAA 7 cut(s) 512, 591, 801, 1061, 1197, 1232, 1923
Tru9I TTAA 7 cut(s) 512, 591, 801, 1061, 1197, 1232, 1923
TscAI CASTG 5 cut(s) 696, 1207, 1558, 2157, 2248
TseFI GTSAC 1 cut(s) 1748
TseI GCWGC 8 cut(s) 227, 503, 1011, 1128, 1535, 1631, 1663, 2156
Tsp45I GTSAC 1 cut(s) 1748
TspDTI ATGAA 3 cut(s) 631, 1991, 2004
TspGWI ACGGA 3 cut(s) 1354, 1738, 1967
TspRI CASTG 5 cut(s) 696, 1207, 1558, 2157, 2248
Tth111I GACNNNGTC 1 cut(s) 1954
Van91I CCANNNNNTGG 1 cut(s) 1890
VpaK11BI GGWCC 3 cut(s) 170, 1337, 1725
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 4 cut(s) 17, 206, 1606, 2344
XceI RCATGY 2 cut(s) 1109, 1844
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XmiI GTMKAC 2 cut(s) 1332, 1542
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 1 cut(s) 438
ZraI GACGTC 1 cut(s) 1519
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.