Rroxscaffold_3G00251120

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014153
Physical Location & Seq
Forward (+)
45047884 .. 45051007
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251120.1

Sequence Viewer

Length: 2307 bp
ATGGCCGTCCAAGTCTCTTGCATTCTCGTCATCTCACACTTCTTTCACATTCTGCTCAAGGCATTGGGTCAACCAGGTCCCATTGCTCAGATCCTAGCTGGTTTGGTGTTAGGTCCTTCGGGATTGTGTAATATAAAGAAAGTAAAGCATTACTTCTTACCCCCTTCAGCTGCACAATACTACGAGATGTTCGGGTTCTTCTGCCGCATCCTCTTCATGTTCCTGTTTGGTTTGGAGGTGGACATTGCTTTCATTAGGCGAAACCTTCGTGTTGTGTGCATACTTGCATGCGGCGGTGCCTCAGTAGGAGCCATTTTTGGGTTGATTTCGTCCTTCTTTTTGCGTGAATACTTTTTGGAAAAATCCCTTGGTAAGGCAAATTATGTTATCGCCATGATGTTATTTGTAGCTTACTCCGCCACCCCAGTCGTCAACCGTCTAGCAGCAGAGCTGAGATTTGCAACCTCAGACATTGGACGCTTGGCGGTGTCTTCTGCACTGGTCATTGAGCTTACTTGCTTACTGATATTCAATTTCATGATCTTGTGCACGTCAGGAAAAATGGTAGGAAACGCCTTCTTAGTCCTAGTAGGGTTGGTGCTTGTGCTCGTTGCTTTCAGGTACATGGCTACATGGTTGAACAAACGCATCAATAACCACAAGTACCTTAGAAACCCTGAGGTGTTTGTCATACTATCGATTCTAATAGCGACTTCTATGCTGATTGAACTGGCCAATTTCAACAGCATCATAGCTTGCTTTCTTGCTGGCGTGGCATTCCCTAAAGAAGGGAAAACGGCAAGAACATTACTACATAAACTCACTTACTCGGTTCACAACTTTGTACTTCCTATTTATTTTGGGTACATCGGCTTCCAATTTGACGGGTCTTATTTTAGTAACATGTCCAACATATTGATTGTTCTCGTGCTGGTGCTGTTGTGCATCTGCAGCAAGATTGGTGGCGTTCTTGCAGTTTGCCACTATTTGAAGATTCCTTTCAGTGAAGGGGTTTTCCTTGCTTTTGTATTGAACTTGAAGGGGCATGCTGATCTCTTATTCATAGGCACCGCGGCCAAAAAACTATTTGAATGGACCCCTATGGCTTACAATTTGTTAGTAATGACAGTCGTTATTAGCACTGTAATATCAGGGCCAATGGTGGCTATCCTAATGAAAAAAGAATATCAATTGTTCGCTCACACTCACACAACACTGAGTACTGAACTGGAAAAGCCTGAGAATGAGCTTCGAGTGCTGGGATGCGTGTATGGACCTCGTCAAGTAAACGCTATACTCTCAGTGATAGCAACATTAAGAGGTCCTCAAACATCACCCATAATGCCCTACCTGGCTCACCTAATCGAGCTACAAAAGAAACGCAGAACCAATGTGTCGTATCATGAGCTCGAAGATGATGAAATGAGCGATGAGGAAGAGTACGGTGGCAATGATGTGGTGGAAATCCATAGGGCAGTTGACGCCTTTACTGTAGAGACCAGCATTCTAATCAACATAATCAAAACCGTGTCAACTTTAGCTGGCTTGTATGAGGACGTGTGCACTGCTGCTGAGGACTTGCGCACAACGATCATTTTGTTGCCTTTTCACAAGCATCAGCGCATTGATGGGAAGATGGAGATTGGGAAACAGGCTGTACGGACGACGAACCAGAAGATTCTTCGCCATGCACCTAGTACTGTTGGGATTATTGTGGAGAAAGGGCTAGCTGGATCTTTAGGGTTCTCGCAGCTGACTAGGGTGGACACTGTCCAGCATGTTGCAACTCTATTTTTCGGAGGGCCTGATGATCGTGAAGCTATTGCGTGGAGTACTCGAATTGCAAATCATACAAAGATTAATTTGACGGTGATTAGATTCCTGCCTACAGAATCATCAAACACGAGGAATATGAAGGTAGACTCGGAGAAGGGGAACAATGACATTGAGGTCTTCATGGCGTTGTCAAGTTTGGAAATGGGCAATGATATTGACAATTCCTTTCTTAATGACTTCTATAACGGGTTTGTTGCTTCTGGTAAAGTTGGATATGTTGAGAAGTATGTAAACAATGGGACAGAAACAGTGGCAGCTCTACGAGACATTGGGGACTTGTACACGCTGTTCATAGTGGGGCGAGGTGGGCGAGGACACTCACCATTAACCACCCAAATGAGTGATTGGGAAGAATGTCCTGAACTGGGAACGGTTGGAGATCTTTTGGCTTCTTCAGACTTCAACGTCAGTGGTTCAATTTTGGTAGTTCAACAACATAGACACACGAAGAAAGACCTTATAGATGATTAG

Protein Analysis

768

Amino Acids

85.02

Weight (kDa)

7.06

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 11 - 394 4.1e-39 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 491 - 579 6.8e-11 Plant cation/H(+) antiporter domain
CHX17_C PF23259 594 - 758 4.4e-14 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16380 AT1G79400
fragaria_vesca FvH4_5g20830 FvH4_5g20830
malus_domestica MD06G1082400.v1.1
prunus_persica Prupe.5G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0207831
rosa_laevigata RLG00000003284
rosa_multiflora Rmu_sc0004647.1_g000005
rosa_roxburghii Rroxscaffold_3G00251120
rosa_rugosa Rorug07G0099700 Rorug07G0099800
rosa_samantha Rh7AG233000 Rh7BG228300 Rh7CG248000 Rh7DG239000
rosa_wichuraiana Rw7G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1949, 2240
Acc16I TGCGCA 1 cut(s) 1583
AccB1I GGYRCC 2 cut(s) 296, 1067
AccI GTMKAC 1 cut(s) 1920
AccII CGCG 1 cut(s) 1073
AciI CCGC 7 cut(s) 205, 291, 294, 417, 485, 1071, 1073
AclWI GGATC 2 cut(s) 85, 1741
AcoI YGGCCR 3 cut(s) 3, 732, 1074
AcuI CTGAAG 2 cut(s) 150, 2214
AcyI GRCGYC 1 cut(s) 1482
AfiI CCNNNNNNNGG 4 cut(s) 318, 373, 788, 2201
AflIII ACRYGT 2 cut(s) 903, 1557
AjiI CACGTC 2 cut(s) 552, 1558
AjnI CCWGG 2 cut(s) 73, 1350
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
Alw21I GWGCWC 4 cut(s) 551, 609, 1410, 1565
Alw26I GTCTC 3 cut(s) 19, 1490, 2094
Alw44I GTGCAC 2 cut(s) 547, 1561
AlwI GGATC 2 cut(s) 85, 1741
AoxI GGCC 5 cut(s) 3, 732, 1074, 1154, 1802
ApaLI GTGCAC 2 cut(s) 547, 1561
ApeKI GCWGC 6 cut(s) 170, 443, 951, 1568, 1750, 2090
AseI ATTAAT 1 cut(s) 1860
AspLEI GCGC 2 cut(s) 1584, 1623
AspS9I GGNCC 7 cut(s) 77, 113, 1095, 1154, 1274, 1322, 1802
AsuHPI GGTGA 4 cut(s) 1326, 1349, 1882, 2148
AsuNHI GCTAGC 1 cut(s) 1726
AvaII GGWCC 5 cut(s) 77, 113, 1095, 1274, 1322
AxyI CCTNAGG 1 cut(s) 678
BaeGI GKGCMC 2 cut(s) 551, 1565
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 2 cut(s) 296, 1067
BanII GRGCYC 1 cut(s) 1410
BarI GAAGNNNNNNTAC 2 cut(s) 857, 889
BauI CACGAG 2 cut(s) 926, 1903
BbsI GAAGAC 2 cut(s) 483, 1945
Bbv12I GWGCWC 4 cut(s) 551, 609, 1410, 1565
BbvCI CCTCAGC 1 cut(s) 1572
BbvI GCAGC 6 cut(s) 157, 455, 963, 1555, 1762, 2102
BccI CCATC 2 cut(s) 1622, 1630
BceAI ACGGC 1 cut(s) 813
BcgI CGANNNNNNTGC 2 cut(s) 700, 734
BciT130I CCWGG 2 cut(s) 75, 1352
BcoDI GTCTC 3 cut(s) 19, 1490, 2094
BfaI CTAG 6 cut(s) 95, 440, 587, 1695, 1727, 1758
BfmI CTRYAG 3 cut(s) 949, 1491, 1887
BglII AGATCT 1 cut(s) 2215
BisI GCNGC 9 cut(s) 171, 205, 292, 444, 952, 1074, 1569, 1751, 2091
BlsI GCNGC 9 cut(s) 172, 206, 293, 445, 953, 1075, 1570, 1752, 2092
BmcAI AGTACT 3 cut(s) 1222, 1699, 1834
Bme1390I CCNGG 2 cut(s) 75, 1352
Bme18I GGWCC 5 cut(s) 77, 113, 1095, 1274, 1322
BmgBI CACGTC 2 cut(s) 552, 1558
BmgT120I GGNCC 7 cut(s) 77, 113, 1095, 1154, 1274, 1322, 1802
BmiI GGNNCC 5 cut(s) 79, 298, 310, 1069, 1097
BmrFI CCNGG 2 cut(s) 75, 1352
BmrI ACTGGG 2 cut(s) 419, 2210
BmsI GCATC 6 cut(s) 216, 657, 756, 954, 1253, 1624
BmtI GCTAGC 1 cut(s) 1730
BmuI ACTGGG 2 cut(s) 419, 2210
BpiI GAAGAC 2 cut(s) 483, 1945
Bpu10I CCTNAGC 1 cut(s) 1572
BpuEI CTTGAG 1 cut(s) 41
Bsa29I ATCGAT 1 cut(s) 698
BsaHI GRCGYC 1 cut(s) 1482
BsaI GGTCTC 1 cut(s) 1490
BsaJI CCNNGG 2 cut(s) 367, 1071
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 373, 788, 2201
Bse1I ACTGG 5 cut(s) 425, 504, 735, 1233, 2205
Bse21I CCTNAGG 1 cut(s) 678
Bse3DI GCAATG 4 cut(s) 81, 243, 1456, 1990
BseBI CCWGG 2 cut(s) 75, 1352
BseCI ATCGAT 1 cut(s) 698
BseDI CCNNGG 2 cut(s) 367, 1071
BseGI GGATG 2 cut(s) 207, 1268
BseLI CCNNNNNNNGG 4 cut(s) 318, 373, 788, 2201
BseMI GCAATG 4 cut(s) 81, 243, 1456, 1990
BseMII CTCAG 9 cut(s) 101, 315, 443, 480, 669, 1208, 1230, 1314, 1563
BseNI ACTGG 5 cut(s) 425, 504, 735, 1233, 2205
BseSI GKGCMC 2 cut(s) 551, 1565
BseXI GCAGC 6 cut(s) 157, 455, 963, 1555, 1762, 2102
BseYI CCCAGC 1 cut(s) 1258
BsgI GTGCAG 2 cut(s) 156, 480
Bsh1236I CGCG 1 cut(s) 1073
BshFI GGCC 5 cut(s) 5, 734, 1076, 1156, 1804
BshNI GGYRCC 2 cut(s) 296, 1067
BshVI ATCGAT 1 cut(s) 698
BsiHKAI GWGCWC 4 cut(s) 551, 609, 1410, 1565
BslFI GGGAC 3 cut(s) 63, 2089, 2123
BslI CCNNNNNNNGG 4 cut(s) 318, 373, 788, 2201
BsmAI GTCTC 3 cut(s) 19, 1490, 2094
BsmFI GGGAC 3 cut(s) 63, 2089, 2123
BsmI GAATGC 3 cut(s) 21, 776, 1503
BsnI GGCC 5 cut(s) 5, 734, 1076, 1156, 1804
Bso31I GGTCTC 1 cut(s) 1490
Bsp1286I GDGCHC 4 cut(s) 551, 609, 1410, 1565
Bsp1407I TGTACA 1 cut(s) 2115
Bsp143I GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
BspACI CCGC 7 cut(s) 205, 291, 294, 417, 485, 1071, 1073
BspANI GGCC 5 cut(s) 5, 734, 1076, 1156, 1804
BspCNI CTCAG 9 cut(s) 100, 314, 444, 479, 670, 1209, 1231, 1313, 1564
BspDI ATCGAT 1 cut(s) 698
BspFNI CGCG 1 cut(s) 1073
BspHI TCATGA 2 cut(s) 537, 1402
BspLI GGNNCC 5 cut(s) 79, 298, 310, 1069, 1097
BspMAI CTGCAG 1 cut(s) 953
BspOI GCTAGC 1 cut(s) 1730
BspPI GGATC 2 cut(s) 85, 1741
BspT107I GGYRCC 2 cut(s) 296, 1067
BspTNI GGTCTC 1 cut(s) 1490
BsrDI GCAATG 4 cut(s) 81, 243, 1456, 1990
BsrGI TGTACA 1 cut(s) 2115
BsrI ACTGG 5 cut(s) 425, 504, 735, 1233, 2205
BssECI CCNNGG 2 cut(s) 367, 1071
BssMI GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
BssNI GRCGYC 1 cut(s) 1482
BssSI CACGAG 2 cut(s) 926, 1903
BssT1I CCWWGG 1 cut(s) 367
Bst2BI CACGAG 2 cut(s) 926, 1903
Bst2UI CCWGG 2 cut(s) 75, 1352
Bst6I CTCTTC 2 cut(s) 218, 1431
BstACI GRCGYC 1 cut(s) 1482
BstAUI TGTACA 1 cut(s) 2115
BstC8I GCNNGC 6 cut(s) 289, 757, 769, 1047, 1543, 1728
BstDSI CCRYGG 1 cut(s) 1071
BstENI CCTNNNNNAGG 2 cut(s) 371, 786
BstF5I GGATG 2 cut(s) 207, 1268
BstFNI CGCG 1 cut(s) 1073
BstHHI GCGC 2 cut(s) 1584, 1623
BstKTI GATC 7 cut(s) 93, 543, 1054, 1593, 1736, 1813, 2218
BstMAI GTCTC 3 cut(s) 19, 1490, 2094
BstMBI GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
BstMWI GCNNNNNNNGC 6 cut(s) 416, 773, 951, 1255, 1481, 2143
BstNI CCWGG 2 cut(s) 75, 1352
BstNSI RCATGY 4 cut(s) 291, 907, 1049, 1781
BstSCI CCNGG 2 cut(s) 73, 1350
BstSFI CTRYAG 3 cut(s) 949, 1491, 1887
BstSLI GKGCMC 2 cut(s) 551, 1565
BstUI CGCG 1 cut(s) 1073
BstV1I GCAGC 6 cut(s) 157, 455, 963, 1555, 1762, 2102
BstV2I GAAGAC 2 cut(s) 483, 1945
BstX2I RGATCY 3 cut(s) 90, 1733, 2215
BstYI RGATCY 3 cut(s) 90, 1733, 2215
Bsu15I ATCGAT 1 cut(s) 698
Bsu36I CCTNAGG 1 cut(s) 678
BsuRI GGCC 5 cut(s) 5, 734, 1076, 1156, 1804
BsuTUI ATCGAT 1 cut(s) 698
BtgI CCRYGG 1 cut(s) 1071
BtgZI GCGATG 1 cut(s) 1443
BtrI CACGTC 2 cut(s) 552, 1558
BtsCI GGATG 2 cut(s) 207, 1268
BtsI GCAGTG 1 cut(s) 1563
BtsIMutI CAGTG 9 cut(s) 497, 1009, 1140, 1214, 1308, 1563, 1767, 2091, 2251
Cac8I GCNNGC 6 cut(s) 289, 757, 769, 1047, 1543, 1728
CciI TCATGA 2 cut(s) 537, 1402
CfoI GCGC 2 cut(s) 1584, 1623
Cfr13I GGNCC 7 cut(s) 77, 113, 1095, 1154, 1274, 1322, 1802
Cfr42I CCGCGG 1 cut(s) 1074
ClaI ATCGAT 1 cut(s) 698
CseI GACGC 2 cut(s) 486, 1490
CsiI ACCWGGT 1 cut(s) 73
CspCI CAANNNNNGTGG 4 cut(s) 943, 978, 2227, 2262
DpnI GATC 7 cut(s) 92, 542, 1053, 1592, 1735, 1812, 2217
DpnII GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
DrdI GACNNNNNNGTC 2 cut(s) 1949, 2240
DseDI GACNNNNNNGTC 2 cut(s) 1949, 2240
EaeI YGGCCR 3 cut(s) 3, 732, 1074
Eam1104I CTCTTC 2 cut(s) 218, 1431
EarI CTCTTC 2 cut(s) 218, 1431
EciI GGCGGA 1 cut(s) 406
Ecl136II GAGCTC 1 cut(s) 1408
Eco130I CCWWGG 1 cut(s) 367
Eco24I GRGCYC 1 cut(s) 1410
Eco31I GGTCTC 1 cut(s) 1490
Eco47I GGWCC 5 cut(s) 77, 113, 1095, 1274, 1322
Eco53kI GAGCTC 1 cut(s) 1408
Eco57I CTGAAG 2 cut(s) 150, 2214
Eco81I CCTNAGG 1 cut(s) 678
EcoICRI GAGCTC 1 cut(s) 1408
EcoNI CCTNNNNNAGG 2 cut(s) 371, 786
EcoO109I RGGNCCY 4 cut(s) 77, 113, 1322, 1802
EcoRII CCWGG 2 cut(s) 73, 1350
EcoT14I CCWWGG 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 1410
ErhI CCWWGG 1 cut(s) 367
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 3 cut(s) 63, 2089, 2123
FblI GTMKAC 1 cut(s) 1920
Fnu4HI GCNGC 9 cut(s) 171, 205, 292, 444, 952, 1074, 1569, 1751, 2091
FokI GGATG 2 cut(s) 194, 1275
FriOI GRGCYC 1 cut(s) 1410
Fsp4HI GCNGC 9 cut(s) 171, 205, 292, 444, 952, 1074, 1569, 1751, 2091
FspBI CTAG 6 cut(s) 95, 440, 587, 1695, 1727, 1758
FspI TGCGCA 1 cut(s) 1583
GlaI GCGC 2 cut(s) 1583, 1622
GluI GCNGC 9 cut(s) 171, 205, 292, 444, 952, 1074, 1569, 1751, 2091
GsaI CCCAGC 1 cut(s) 1262
HaeIII GGCC 5 cut(s) 5, 734, 1076, 1156, 1804
HgaI GACGC 2 cut(s) 486, 1490
HhaI GCGC 2 cut(s) 1584, 1623
Hin1I GRCGYC 1 cut(s) 1482
Hin6I GCGC 2 cut(s) 1582, 1621
HinP1I GCGC 2 cut(s) 1582, 1621
HincII GTYRAC 4 cut(s) 71, 433, 1480, 1533
HindII GTYRAC 4 cut(s) 71, 433, 1480, 1533
HinfI GANTC 6 cut(s) 700, 994, 1678, 1878, 1892, 1922
HphI GGTGA 4 cut(s) 1326, 1349, 1882, 2148
Hpy188I TCNGA 5 cut(s) 90, 469, 1799, 1927, 2233
Hpy188III TCNNGA 6 cut(s) 120, 538, 555, 1403, 1814, 2195
Hpy99I CGWCG 1 cut(s) 1669
HpyCH4IV ACGT 3 cut(s) 551, 1557, 2241
HpyF10VI GCNNNNNNNGC 6 cut(s) 416, 773, 951, 1255, 1481, 2143
HpySE526I ACGT 3 cut(s) 551, 1557, 2241
Hsp92I GRCGYC 1 cut(s) 1482
HspAI GCGC 2 cut(s) 1582, 1621
KspI CCGCGG 1 cut(s) 1074
Kzo9I GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 6 cut(s) 157, 455, 963, 1555, 1762, 2102
LweI GCATC 6 cut(s) 216, 657, 756, 954, 1253, 1624
MabI ACCWGGT 1 cut(s) 73
MaeI CTAG 6 cut(s) 95, 440, 587, 1695, 1727, 1758
MaeII ACGT 3 cut(s) 551, 1557, 2241
MaeIII GTNAC 1 cut(s) 899
MalI GATC 7 cut(s) 92, 542, 1053, 1592, 1735, 1812, 2217
MboI GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
MfeI CAATTG 1 cut(s) 1190
MflI RGATCY 3 cut(s) 90, 1733, 2215
MhlI GDGCHC 4 cut(s) 551, 609, 1410, 1565
MlsI TGGCCA 1 cut(s) 734
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 1 cut(s) 1916
MmeI TCCRAC 3 cut(s) 933, 2026, 2191
Mox20I TGGCCA 1 cut(s) 734
MscI TGGCCA 1 cut(s) 734
MseI TTAA 4 cut(s) 1316, 1860, 2007, 2162
MslI CAYNNNNRTG 1 cut(s) 2171
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 3 cut(s) 170, 1073, 1753
MspR9I CCNGG 2 cut(s) 75, 1352
MunI CAATTG 1 cut(s) 1190
Mva1269I GAATGC 3 cut(s) 21, 776, 1503
MvaI CCWGG 2 cut(s) 75, 1352
MvnI CGCG 1 cut(s) 1073
MwoI GCNNNNNNNGC 6 cut(s) 416, 773, 951, 1255, 1481, 2143
NdeII GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
NheI GCTAGC 1 cut(s) 1726
NlaIV GGNNCC 5 cut(s) 79, 298, 310, 1069, 1097
NsbI TGCGCA 1 cut(s) 1583
NspI RCATGY 4 cut(s) 291, 907, 1049, 1781
PaeI GCATGC 2 cut(s) 291, 1049
PagI TCATGA 2 cut(s) 537, 1402
PciI ACATGT 1 cut(s) 903
PctI GAATGC 3 cut(s) 21, 776, 1503
PfeI GAWTC 5 cut(s) 700, 994, 1678, 1878, 1892
PflFI GACNNNGTC 2 cut(s) 1278, 1769
PkrI GCNGC 9 cut(s) 172, 206, 293, 445, 953, 1075, 1570, 1752, 2092
PleI GAGTC 1 cut(s) 1916
PpsI GAGTC 1 cut(s) 1916
PpuMI RGGWCCY 3 cut(s) 77, 113, 1322
PscI ACATGT 1 cut(s) 903
PshBI ATTAAT 1 cut(s) 1860
Psp124BI GAGCTC 1 cut(s) 1410
Psp5II RGGWCCY 3 cut(s) 77, 113, 1322
Psp6I CCWGG 2 cut(s) 73, 1350
PspFI CCCAGC 1 cut(s) 1258
PspGI CCWGG 2 cut(s) 73, 1350
PspN4I GGNNCC 5 cut(s) 79, 298, 310, 1069, 1097
PspPI GGNCC 7 cut(s) 77, 113, 1095, 1154, 1274, 1322, 1802
PspPPI RGGWCCY 3 cut(s) 77, 113, 1322
PsrI GAACNNNNNNTAC 2 cut(s) 2108, 2140
PstI CTGCAG 1 cut(s) 953
PsuI RGATCY 3 cut(s) 90, 1733, 2215
PsyI GACNNNGTC 2 cut(s) 1278, 1769
PvuII CAGCTG 2 cut(s) 170, 1753
RseI CAYNNNNRTG 1 cut(s) 2171
SacI GAGCTC 1 cut(s) 1410
SacII CCGCGG 1 cut(s) 1074
SaqAI TTAA 4 cut(s) 1316, 1860, 2007, 2162
SatI GCNGC 9 cut(s) 171, 205, 292, 444, 952, 1074, 1569, 1751, 2091
Sau3AI GATC 7 cut(s) 90, 540, 1051, 1590, 1733, 1810, 2215
Sau96I GGNCC 7 cut(s) 77, 113, 1095, 1154, 1274, 1322, 1802
ScaI AGTACT 3 cut(s) 1222, 1699, 1834
SchI GAGTC 1 cut(s) 1916
ScrFI CCNGG 2 cut(s) 75, 1352
SduI GDGCHC 4 cut(s) 551, 609, 1410, 1565
SexAI ACCWGGT 1 cut(s) 73
SfaNI GCATC 6 cut(s) 216, 657, 756, 954, 1253, 1624
SfcI CTRYAG 3 cut(s) 949, 1491, 1887
Sfr303I CCGCGG 1 cut(s) 1074
SgrBI CCGCGG 1 cut(s) 1074
SinI GGWCC 5 cut(s) 77, 113, 1095, 1274, 1322
SmiMI CAYNNNNRTG 1 cut(s) 2171
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SphI GCATGC 2 cut(s) 291, 1049
SsiI CCGC 7 cut(s) 205, 291, 294, 417, 485, 1071, 1073
SspMI CTAG 6 cut(s) 95, 440, 587, 1695, 1727, 1758
SstI GAGCTC 1 cut(s) 1410
StyD4I CCNGG 2 cut(s) 73, 1350
StyI CCWWGG 1 cut(s) 367
TaiI ACGT 3 cut(s) 554, 1560, 2244
TaqI TCGA 5 cut(s) 698, 1252, 1365, 1410, 1837
TatI WGTACW 5 cut(s) 844, 1220, 1697, 1832, 2115
TauI GCSGC 3 cut(s) 207, 294, 1076
TfiI GAWTC 5 cut(s) 700, 994, 1678, 1878, 1892
Tru1I TTAA 4 cut(s) 1316, 1860, 2007, 2162
Tru9I TTAA 4 cut(s) 1316, 1860, 2007, 2162
TscAI CASTG 9 cut(s) 504, 1009, 1147, 1221, 1308, 1570, 1774, 2091, 2251
TseI GCWGC 6 cut(s) 170, 443, 951, 1568, 1750, 2090
TspDTI ATGAA 9 cut(s) 205, 241, 526, 1051, 1190, 1434, 1928, 1945, 2116
TspGWI ACGGA 1 cut(s) 1675
TspRI CASTG 9 cut(s) 504, 1009, 1147, 1221, 1308, 1570, 1774, 2091, 2251
Tth111I GACNNNGTC 2 cut(s) 1278, 1769
VneI GTGCAC 2 cut(s) 547, 1561
VpaK11BI GGWCC 5 cut(s) 77, 113, 1095, 1274, 1322
VspI ATTAAT 1 cut(s) 1860
XagI CCTNNNNNAGG 2 cut(s) 371, 786
XceI RCATGY 4 cut(s) 291, 907, 1049, 1781
XmiI GTMKAC 1 cut(s) 1920
XspI CTAG 6 cut(s) 95, 440, 587, 1695, 1727, 1758
ZrmI AGTACT 3 cut(s) 1222, 1699, 1834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.