FvH4_5g21360

S-norcoclaurine synthase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016346
Physical Location & Seq
Forward (+)
12876198 .. 12882248
6051 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21360.t1

Sequence Viewer

Length: 1629 bp
ATGGAGACTGAGGTACTCATTAGAAAAGACCAGCTGGGTGGTTCTCTCCCAGTTGAGAATGTTCAAGCACTGGCTTCCAATAACTTGAAGGACATCCCAAGTCGATATCTCAGGCCCGAAGCTGAGTTTGAACAAGTTTCCATAGAGGAGTCGCTTCAAATTCCAGTGGTTGATATGCGCAAGCTGATAGAGAATTCATCGTTCCGCGATGATGAATTGGCAAAACTTCATTTGGCTTGTAAGGAATGGGGCTTCTTTCAGTTGATCAATCATGGGGTATCAGATGAAGTGATTGAGAAAATGAAGACCGACACAGAAGAGTTCTTCAGGCAGCCATTAGAAGCAAAGAAGGCTTATGCAACACAGCCAAATCATATCGAAGGCTACGGCCAAGCCTTTGTTGTTTCGGAAGAGCAAAAACTTGACTGGGGAGACATGCTCTTCCTTCTCTCTCAACCTGTCCGCCAAAGGACTCTCACATTCTGGCCCACCCTTCCCACTACTTTCAGGGAGGCATTGGATAAATACTCACAAGAGCTCCAAAAAGTAACAATCTACCTACTGAAGTCCATATCCAGTAACTTAGGAGTCAATTTAGAGATGGAAAGAATGTTTGATGATGGAGTACAAGGAGTAAGAATGAACTACTACCCACCCTGTCCACAGGCAAACAAAGTAATTGGTCTCACTCCACACTCTGATGCTGTGGCTTTAACTCTACTACTACAAGTTAACGAAGTTCAAGGACTACAAATCAGGAAAAATGGAAAATGGGTACCTGTAGAACCTATAGTTGGTGCATTCATCGTCAACATTGGCGACTTCACTGAGATAATGAGTAATGGAGTATATAAGAGCATAGAGCACAGAGCAGTGGTGAACCCAGAGAAGGAACGCCTTTCTATTGCAGCATTCCACAGCCCTAATATTGGGACCATGATTGGCCCTTTACCGGATCTGGTCAAGGGGAATGCAGAAAAGTACAAGACTTCAAGCTATGATGAGTATCTTAAACTTGTCGTGACCAGTAAGCTTGATGCTGAGGCATACCTGGTGTCAGTCAAGATTTCAAGCTCTGCCGGGATGGTTCATTATGGATATGAGCATCCCGACGCGTCGGTCGAGTTAGTAGGGAAGAGCAACAGTGGGAGGGAGTTTTATGAGGATGAGGATGATATTGGCGGCGGCGGCAACGGCGGTGGTAGTGGGAGTGGGAAGAAAAGGAGTGATGGATATGAGGGTTGGATGGAACGATTAAAGATTGAGGCTGAAAATGAGAGGAAAGAAGCTGCTGAAAGGGAAGAGAGAATTAGGAATGAAACCTTTGATATGGAGAAAAGACTCTCTGCACAAATGGAGGATTTCAAAAGGCAGAATGATGTCGTCCTCTTCTTCTTTGTCTCCTTCATCGCCTTCTGCTTCTTCTCCTCCGCCTTCTTCCTTCCGACCTCTAAACTCGAAGCTTTGTCCTCTCTTCTTCACCGACTTCTTCTTTCTTCCTTCCAAATGTTTCCCAATCCGAAACATTGCCGCCATGAAACCCAGAAACCGTCATTGAAACACCTAGCAGCCGACTTCACTAAGCAGGAGATGTCAAAATTCTTGGAGACTAAGTTTCCAGATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

543

Amino Acids

61.44

Weight (kDa)

5.35

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 54 - 163 2.4e-31 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 308 8.4e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21360
malus_domestica MD06G1038700.v1.1
prunus_persica Prupe.5G043900_v2.0.a1 Prupe.5G043900_v2.0.a1
pyrus_communis pycom06g03250
rosa_chinensis RchiOBHm_Chr7g0208701
rosa_laevigata RLG00000003198 RLG00000003200
rosa_multiflora Rmu_sc0004103.1_g000007
rosa_roxburghii Rroxscaffold_3G00250300
rosa_rugosa Rorug07G0105500
rosa_samantha Rh7AG240500 Rh7CG255100 Rh7DG246500
rosa_wichuraiana Rw7G020420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1118
Acc16I TGCGCA 1 cut(s) 179
Acc65I GGTACC 1 cut(s) 775
AccB1I GGYRCC 1 cut(s) 775
AccII CGCG 2 cut(s) 207, 1115
AciI CCGC 8 cut(s) 205, 463, 1182, 1185, 1188, 1197, 1431, 1531
AclWI GGATC 1 cut(s) 963
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 3 cut(s) 159, 193, 1598
AcuI CTGAAG 2 cut(s) 310, 584
AfaI GTAC 4 cut(s) 15, 627, 777, 983
AfiI CCNNNNNNNGG 4 cut(s) 794, 889, 929, 952
AflIII ACRYGT 1 cut(s) 1113
AgsI TTSAA 9 cut(s) 65, 88, 131, 158, 743, 993, 1071, 1366, 1558
AjnI CCWGG 1 cut(s) 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 777, 809
AluBI AGCT 9 cut(s) 34, 122, 184, 538, 996, 1033, 1074, 1289, 1463
AluI AGCT 9 cut(s) 34, 122, 184, 538, 996, 1033, 1074, 1289, 1463
Alw21I GWGCWC 2 cut(s) 540, 867
Alw26I GTCTC 4 cut(s) 426, 689, 1405, 1601
AlwI GGATC 1 cut(s) 963
AoxI GGCC 4 cut(s) 113, 388, 485, 943
ApeKI GCWGC 4 cut(s) 331, 908, 1289, 1568
ApoI RAATTY 3 cut(s) 159, 193, 1598
Asp718I GGTACC 1 cut(s) 775
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 4 cut(s) 114, 486, 933, 944
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 2 cut(s) 889, 1472
AvaII GGWCC 1 cut(s) 933
BanI GGYRCC 1 cut(s) 775
BanII GRGCYC 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 2 cut(s) 540, 867
BbvCI CCTCAGC 1 cut(s) 1041
BbvI GCAGC 4 cut(s) 343, 920, 1276, 1580
BccI CCATC 6 cut(s) 595, 614, 1078, 1223, 1240, 1616
BceAI ACGGC 2 cut(s) 403, 1210
BciT130I CCWGG 1 cut(s) 1052
BclI TGATCA 1 cut(s) 264
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 4 cut(s) 426, 689, 1405, 1601
BfaI CTAG 1 cut(s) 1565
BfmI CTRYAG 2 cut(s) 780, 789
BisI GCNGC 8 cut(s) 332, 909, 1183, 1186, 1189, 1290, 1531, 1569
BlsI GCNGC 8 cut(s) 333, 910, 1184, 1187, 1190, 1291, 1532, 1570
Bme1390I CCNGG 2 cut(s) 1052, 1081
Bme18I GGWCC 1 cut(s) 933
BmgT120I GGNCC 4 cut(s) 114, 486, 933, 944
BmiI GGNNCC 2 cut(s) 777, 934
BmrFI CCNGG 2 cut(s) 1052, 1081
BmrI ACTGGG 2 cut(s) 44, 436
BmsI GCATC 3 cut(s) 691, 1027, 1114
BmuI ACTGGG 2 cut(s) 44, 436
BpiI GAAGAC 1 cut(s) 311
BplI GAGNNNNNCTC 2 cut(s) 423, 455
Bpu10I CCTNAGC 1 cut(s) 1041
BpuMI CCSGG 1 cut(s) 1081
BsaBI GATNNNNATC 1 cut(s) 1005
BsaI GGTCTC 1 cut(s) 689
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 1598, 1628
Bsc4I CCNNNNNNNGG 4 cut(s) 794, 889, 929, 952
Bse1I ACTGG 6 cut(s) 50, 75, 164, 431, 576, 1026
Bse3DI GCAATG 1 cut(s) 1525
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 1 cut(s) 1052
BseGI GGATG 6 cut(s) 93, 1089, 1105, 1171, 1177, 1251
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 4 cut(s) 794, 889, 929, 952
BseMI GCAATG 1 cut(s) 1525
BseMII CTCAG 4 cut(s) 114, 124, 819, 1032
BseNI ACTGG 6 cut(s) 50, 75, 164, 431, 576, 1026
BseRI GAGGAG 2 cut(s) 161, 1417
BseXI GCAGC 4 cut(s) 343, 920, 1276, 1580
BseYI CCCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 1332
Bsh1236I CGCG 2 cut(s) 207, 1115
Bsh1285I CGRYCG 1 cut(s) 1122
BshFI GGCC 4 cut(s) 115, 390, 487, 945
BshNI GGYRCC 1 cut(s) 775
BsiEI CGRYCG 1 cut(s) 1122
BsiHKAI GWGCWC 2 cut(s) 540, 867
BsiSI CCGG 2 cut(s) 953, 1080
BslFI GGGAC 1 cut(s) 946
BslI CCNNNNNNNGG 4 cut(s) 794, 889, 929, 952
BsmAI GTCTC 4 cut(s) 426, 689, 1405, 1601
BsmFI GGGAC 1 cut(s) 946
BsmI GAATGC 3 cut(s) 800, 911, 976
BsnI GGCC 4 cut(s) 115, 390, 487, 945
Bso31I GGTCTC 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 540, 867
Bsp143I GATC 2 cut(s) 264, 955
BspACI CCGC 8 cut(s) 205, 463, 1182, 1185, 1188, 1197, 1431, 1531
BspANI GGCC 4 cut(s) 115, 390, 487, 945
BspCNI CTCAG 4 cut(s) 115, 123, 820, 1033
BspFNI CGCG 2 cut(s) 207, 1115
BspLI GGNNCC 2 cut(s) 777, 934
BspPI GGATC 1 cut(s) 963
BspQI GCTCTTC 3 cut(s) 405, 446, 1130
BspT107I GGYRCC 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 1525
BsrI ACTGG 6 cut(s) 50, 75, 164, 431, 576, 1026
BssMI GATC 2 cut(s) 264, 955
Bst2UI CCWGG 1 cut(s) 1052
Bst4CI ACNGT 2 cut(s) 1145, 1551
Bst6I CTCTTC 7 cut(s) 312, 405, 446, 1130, 1296, 1394, 1479
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 8 cut(s) 9, 110, 123, 583, 828, 1041, 1581, 1611
BstF5I GGATG 6 cut(s) 93, 1089, 1105, 1171, 1177, 1251
BstFNI CGCG 2 cut(s) 207, 1115
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 2 cut(s) 267, 958
BstMAI GTCTC 4 cut(s) 426, 689, 1405, 1601
BstMBI GATC 2 cut(s) 264, 955
BstMCI CGRYCG 1 cut(s) 1122
BstMWI GCNNNNNNNGC 3 cut(s) 350, 1188, 1194
BstNI CCWGG 1 cut(s) 1052
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 2 cut(s) 1050, 1079
BstSFI CTRYAG 2 cut(s) 780, 789
BstUI CGCG 2 cut(s) 207, 1115
BstV1I GCAGC 4 cut(s) 343, 920, 1276, 1580
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 955
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 1 cut(s) 955
BsuRI GGCC 4 cut(s) 115, 390, 487, 945
BtgZI GCGATG 2 cut(s) 222, 1393
BtsCI GGATG 6 cut(s) 93, 1089, 1105, 1171, 1177, 1251
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 5 cut(s) 68, 171, 825, 879, 1150
Cac8I GCNNGC 1 cut(s) 182
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 4 cut(s) 114, 486, 933, 944
CseI GACGC 2 cut(s) 1104, 1121
CsiI ACCWGGT 1 cut(s) 1050
Csp6I GTAC 4 cut(s) 14, 626, 776, 982
CspCI CAANNNNNGTGG 2 cut(s) 1180, 1215
CviAII CATG 4 cut(s) 272, 436, 937, 1535
CviQI GTAC 4 cut(s) 14, 626, 776, 982
DdeI CTNAG 8 cut(s) 9, 110, 123, 583, 828, 1041, 1581, 1611
DpnI GATC 2 cut(s) 266, 957
DpnII GATC 2 cut(s) 264, 955
DrdI GACNNNNNNGTC 1 cut(s) 1118
DseDI GACNNNNNNGTC 1 cut(s) 1118
EaeI YGGCCR 1 cut(s) 388
Eam1104I CTCTTC 7 cut(s) 312, 405, 446, 1130, 1296, 1394, 1479
EarI CTCTTC 7 cut(s) 312, 405, 446, 1130, 1296, 1394, 1479
EciI GGCGGA 2 cut(s) 452, 1420
Ecl136II GAGCTC 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 540
Eco31I GGTCTC 1 cut(s) 689
Eco32I GATATC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 933
Eco53kI GAGCTC 1 cut(s) 538
Eco57I CTGAAG 2 cut(s) 310, 584
EcoICRI GAGCTC 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 1050
EcoRV GATATC 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 540
FaeI CATG 4 cut(s) 275, 439, 940, 1538
FaqI GGGAC 1 cut(s) 946
FatI CATG 4 cut(s) 271, 435, 936, 1534
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 8 cut(s) 332, 909, 1183, 1186, 1189, 1290, 1531, 1569
FokI GGATG 6 cut(s) 80, 1092, 1096, 1178, 1184, 1258
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 8 cut(s) 332, 909, 1183, 1186, 1189, 1290, 1531, 1569
FspBI CTAG 1 cut(s) 1565
FspI TGCGCA 1 cut(s) 179
GlaI GCGC 1 cut(s) 179
GluI GCNGC 8 cut(s) 332, 909, 1183, 1186, 1189, 1290, 1531, 1569
GsaI CCCAGC 1 cut(s) 38
HaeIII GGCC 4 cut(s) 115, 390, 487, 945
HapII CCGG 2 cut(s) 953, 1080
HgaI GACGC 2 cut(s) 1104, 1121
HhaI GCGC 1 cut(s) 180
Hin1II CATG 4 cut(s) 275, 439, 940, 1538
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 2 cut(s) 733, 811
HindII GTYRAC 2 cut(s) 733, 811
HindIII AAGCTT 2 cut(s) 1031, 1461
HinfI GANTC 4 cut(s) 149, 472, 588, 1341
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 2 cut(s) 953, 1080
HphI GGTGA 2 cut(s) 889, 1472
Hpy166II GTNNAC 4 cut(s) 662, 733, 811, 880
Hpy188I TCNGA 5 cut(s) 283, 409, 700, 1446, 1521
Hpy188III TCNNGA 5 cut(s) 757, 1021, 1063, 1109, 1619
Hpy8I GTNNAC 4 cut(s) 662, 733, 811, 880
Hpy99I CGWCG 2 cut(s) 1115, 1120
HpyCH4III ACNGT 2 cut(s) 1145, 1551
HpyCH4V TGCA 5 cut(s) 359, 800, 908, 974, 1349
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 1188, 1194
HpyF3I CTNAG 8 cut(s) 9, 110, 123, 583, 828, 1041, 1581, 1611
Hsp92II CATG 4 cut(s) 275, 439, 940, 1538
HspAI GCGC 1 cut(s) 178
KpnI GGTACC 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 264
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 2 cut(s) 264, 955
LguI GCTCTTC 3 cut(s) 405, 446, 1130
LmnI GCTCC 1 cut(s) 543
Lsp1109I GCAGC 4 cut(s) 343, 920, 1276, 1580
LweI GCATC 3 cut(s) 691, 1027, 1114
MabI ACCWGGT 1 cut(s) 1050
MaeI CTAG 1 cut(s) 1565
MaeIII GTNAC 3 cut(s) 547, 578, 1021
MalI GATC 2 cut(s) 266, 957
MboI GATC 2 cut(s) 264, 955
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 2 cut(s) 540, 867
MluCI AATT 7 cut(s) 159, 193, 215, 592, 678, 1308, 1598
MluI ACGCGT 1 cut(s) 1113
MlyI GAGTC 4 cut(s) 158, 466, 597, 1335
MmeI TCCRAC 2 cut(s) 1223, 1469
MseI TTAA 4 cut(s) 713, 732, 1011, 1256
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 2 cut(s) 953, 1080
MspR9I CCNGG 2 cut(s) 1052, 1081
Mva1269I GAATGC 3 cut(s) 800, 911, 976
MvaI CCWGG 1 cut(s) 1052
MvnI CGCG 2 cut(s) 207, 1115
MwoI GCNNNNNNNGC 3 cut(s) 350, 1188, 1194
NciI CCSGG 1 cut(s) 1081
NdeII GATC 2 cut(s) 264, 955
NlaIII CATG 4 cut(s) 275, 439, 940, 1538
NlaIV GGNNCC 2 cut(s) 777, 934
NmuCI GTSAC 1 cut(s) 1021
NsbI TGCGCA 1 cut(s) 179
NspI RCATGY 1 cut(s) 439
PciSI GCTCTTC 3 cut(s) 405, 446, 1130
PcsI WCGNNNNNNNCGW 1 cut(s) 1119
PctI GAATGC 3 cut(s) 800, 911, 976
PkrI GCNGC 8 cut(s) 333, 910, 1184, 1187, 1190, 1291, 1532, 1570
PleI GAGTC 4 cut(s) 157, 466, 596, 1335
PpsI GAGTC 4 cut(s) 157, 466, 596, 1335
Psp124BI GAGCTC 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 1050
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 1 cut(s) 1050
PspN4I GGNNCC 2 cut(s) 777, 934
PspPI GGNCC 4 cut(s) 114, 486, 933, 944
PsuI RGATCY 1 cut(s) 955
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 4 cut(s) 15, 627, 777, 983
RsaNI GTAC 4 cut(s) 14, 626, 776, 982
RseI CAYNNNNRTG 1 cut(s) 699
SacI GAGCTC 1 cut(s) 540
SapI GCTCTTC 3 cut(s) 405, 446, 1130
SaqAI TTAA 4 cut(s) 713, 732, 1011, 1256
SatI GCNGC 8 cut(s) 332, 909, 1183, 1186, 1189, 1290, 1531, 1569
Sau3AI GATC 2 cut(s) 264, 955
Sau96I GGNCC 4 cut(s) 114, 486, 933, 944
SchI GAGTC 4 cut(s) 158, 466, 597, 1335
ScrFI CCNGG 2 cut(s) 1052, 1081
SduI GDGCHC 2 cut(s) 540, 867
SexAI ACCWGGT 1 cut(s) 1050
SfaNI GCATC 3 cut(s) 691, 1027, 1114
SfcI CTRYAG 2 cut(s) 780, 789
SinI GGWCC 1 cut(s) 933
SmiMI CAYNNNNRTG 1 cut(s) 699
Sse9I AATT 7 cut(s) 159, 193, 215, 592, 678, 1308, 1598
SsiI CCGC 8 cut(s) 205, 463, 1182, 1185, 1188, 1197, 1431, 1531
SspI AATATT 1 cut(s) 928
SspMI CTAG 1 cut(s) 1565
SstI GAGCTC 1 cut(s) 540
StyD4I CCNGG 2 cut(s) 1050, 1079
TaaI ACNGT 2 cut(s) 1145, 1551
TaqI TCGA 4 cut(s) 103, 378, 1122, 1458
TaqII GACCGA 2 cut(s) 323, 1108
TasI AATT 7 cut(s) 159, 193, 215, 592, 678, 1308, 1598
TatI WGTACW 2 cut(s) 625, 981
TauI GCSGC 4 cut(s) 1185, 1188, 1191, 1533
Tru1I TTAA 4 cut(s) 713, 732, 1011, 1256
Tru9I TTAA 4 cut(s) 713, 732, 1011, 1256
TscAI CASTG 5 cut(s) 75, 171, 832, 879, 1150
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 4 cut(s) 331, 908, 1289, 1568
Tsp45I GTSAC 1 cut(s) 1021
TspRI CASTG 5 cut(s) 75, 171, 832, 879, 1150
VpaK11BI GGWCC 1 cut(s) 933
XapI RAATTY 3 cut(s) 159, 193, 1598
XceI RCATGY 1 cut(s) 439
XspI CTAG 1 cut(s) 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.