Rw7G020420

S-norcoclaurine synthase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016346
Physical Location & Seq
Forward (+)
23154479 .. 23156159
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020420.1

Sequence Viewer

Length: 1086 bp
ATGGAGACTGAGCTACTCATTAGAAAAGACCTGGGTGGTTCTCTCCCAGTTGAGAATGTTCAAGCCCTGGCTTCAAACAACTTGAAGGACATCCCGCGCCGATATCTCAGGCCTGAAGCTGAGATTGAACAAATTTCCATTGAGGACTCGCTTCAAATTCCAGTGGTTGATATGGACAAGCTAATGGGGAATCCATTGTTCCATGATGATGAATTGGCAAAACTTCATTTGGCTTGTAAGGAATGGGGCTTCTTTCAGCTGATCAATCATGGGGTATCAGAAGAAGTGATTGAGAAAATGAAGACTGACACACAGGAGTTCTTCGGGCTGCCATTACAAGTAAAGAAGGCCTATGCAACACAGCCGAATCACATCGAAGGCTATGGCCAAGCCTTTGTTGTTTCGGAAGAGCAAAAACTTGACTGGGGAGACATGCTCTTCCTTCTCTCTCAACCAGTCCGCCAAAGGACTCTCACATTCTGGCCTACCCTTCCCACTTCTTTCAGGGAGACATTGGACAAGTATTCACAGGAGCTGCAACGATTAACGATCTACCTATTGAAGTCCATATCTAGTAACCTAGGAGTCAATTTAGAGAAAATGGAAAGCATGTTTGATGATGGAGTACAAGGAGTAAGAATGAACTACTACCCACCCTGTCCACAGGCAAACAAAGTTATCGGTCTCACTCCACACTCTGATGCTGTGGGTTTGACTCTACTACTGCAAGTTAATGAAGTTCAAGGATTACAAATCAGGAAAAATGGAAAATGGGTACCAGTAAAACCTGCAGTTGGTGCATTCATCGTCAACGTGGGAGACATCATTGAGATAATGAGTAATGGAGCATATAAGAGCATAGAGCACAGAGCAGTGGTGAACCCAGAGAAGGAACGCCTTTCAATTGCGGCATTCCACAGCCCAAATATTGGGACCATGATTGGTCCTTTACCGGATCTGATCAAGGAGAATGCAGCAAACTACAAGACTATGAGCAATGAGGAGTATATTAAGCTTATAGTGACCAGTAAACTTGATGGTAAAACTCTGCTGGACCAAATGAGATTGAACCAGCAACTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

361

Amino Acids

40.81

Weight (kDa)

5.49

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 162 1.2e-30 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 308 1.5e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21360
malus_domestica MD06G1038700.v1.1
prunus_persica Prupe.5G043900_v2.0.a1 Prupe.5G043900_v2.0.a1
pyrus_communis pycom06g03250
rosa_chinensis RchiOBHm_Chr7g0208701
rosa_laevigata RLG00000003198 RLG00000003200
rosa_multiflora Rmu_sc0004103.1_g000007
rosa_roxburghii Rroxscaffold_3G00250300
rosa_rugosa Rorug07G0105500
rosa_samantha Rh7AG240500 Rh7CG255100 Rh7DG246500
rosa_wichuraiana Rw7G020420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 796
Acc65I GGTACC 1 cut(s) 775
AccB1I GGYRCC 1 cut(s) 775
AccB7I CCANNNNNTGG 1 cut(s) 929
AccII CGCG 1 cut(s) 97
AciI CCGC 3 cut(s) 95, 460, 908
AclWI GGATC 1 cut(s) 963
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 2 cut(s) 132, 156
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 2 cut(s) 627, 777
AfiI CCNNNNNNNGG 3 cut(s) 794, 889, 929
AgsI TTSAA 9 cut(s) 62, 75, 85, 128, 155, 562, 743, 903, 1069
AjnI CCWGG 2 cut(s) 30, 66
AluBI AGCT 6 cut(s) 13, 119, 181, 259, 535, 1015
AluI AGCT 6 cut(s) 13, 119, 181, 259, 535, 1015
Alw21I GWGCWC 1 cut(s) 867
Alw26I GTCTC 4 cut(s) 423, 503, 689, 813
AlwI GGATC 1 cut(s) 963
AlwNI CAGNNNCTG 1 cut(s) 535
AoxI GGCC 4 cut(s) 110, 348, 385, 482
ApeKI GCWGC 3 cut(s) 328, 535, 974
ApoI RAATTY 2 cut(s) 132, 156
Asp718I GGTACC 1 cut(s) 775
AspA2I CCTAGG 1 cut(s) 580
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 3 cut(s) 933, 944, 1054
AsuHPI GGTGA 1 cut(s) 889
AvaII GGWCC 3 cut(s) 933, 944, 1054
AvrII CCTAGG 1 cut(s) 580
BalI TGGCCA 1 cut(s) 387
BanI GGYRCC 1 cut(s) 775
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 867
BbvI GCAGC 3 cut(s) 315, 522, 986
BccI CCATC 2 cut(s) 614, 1031
BciT130I CCWGG 2 cut(s) 32, 68
BclI TGATCA 2 cut(s) 261, 960
BcoDI GTCTC 4 cut(s) 423, 503, 689, 813
BfaI CTAG 2 cut(s) 573, 581
BfmI CTRYAG 1 cut(s) 789
BfuAI ACCTGC 1 cut(s) 796
BisI GCNGC 4 cut(s) 329, 536, 909, 975
BlnI CCTAGG 1 cut(s) 580
BlsI GCNGC 4 cut(s) 330, 537, 910, 976
Bme1390I CCNGG 2 cut(s) 32, 68
Bme18I GGWCC 3 cut(s) 933, 944, 1054
BmgT120I GGNCC 3 cut(s) 933, 944, 1054
BmiI GGNNCC 2 cut(s) 777, 934
BmrFI CCNGG 2 cut(s) 32, 68
BmrI ACTGGG 2 cut(s) 41, 433
BmsI GCATC 1 cut(s) 691
BmuI ACTGGG 2 cut(s) 41, 433
BpiI GAAGAC 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 420, 452
BsaI GGTCTC 1 cut(s) 689
BsaJI CCNNGG 3 cut(s) 31, 66, 580
BsaWI WCCGGW 1 cut(s) 952
Bsc4I CCNNNNNNNGG 3 cut(s) 794, 889, 929
Bse1I ACTGG 6 cut(s) 47, 161, 428, 455, 779, 1026
Bse3DI GCAATG 1 cut(s) 1003
BseBI CCWGG 2 cut(s) 32, 68
BseDI CCNNGG 3 cut(s) 31, 66, 580
BseGI GGATG 1 cut(s) 90
BseLI CCNNNNNNNGG 3 cut(s) 794, 889, 929
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 2 cut(s) 111, 121
BseNI ACTGG 6 cut(s) 47, 161, 428, 455, 779, 1026
BseRI GAGGAG 1 cut(s) 1016
BseXI GCAGC 3 cut(s) 315, 522, 986
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 4 cut(s) 112, 350, 387, 484
BshNI GGYRCC 1 cut(s) 775
BsiHKAI GWGCWC 1 cut(s) 867
BsiSI CCGG 1 cut(s) 953
BslFI GGGAC 1 cut(s) 946
BslI CCNNNNNNNGG 3 cut(s) 794, 889, 929
BsmAI GTCTC 4 cut(s) 423, 503, 689, 813
BsmFI GGGAC 1 cut(s) 946
BsmI GAATGC 3 cut(s) 800, 911, 976
BsnI GGCC 4 cut(s) 112, 350, 387, 484
Bso31I GGTCTC 1 cut(s) 689
Bsp1286I GDGCHC 1 cut(s) 867
Bsp143I GATC 4 cut(s) 261, 549, 955, 960
BspACI CCGC 3 cut(s) 95, 460, 908
BspANI GGCC 4 cut(s) 112, 350, 387, 484
BspCNI CTCAG 2 cut(s) 112, 120
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 2 cut(s) 777, 934
BspMAI CTGCAG 1 cut(s) 793
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 1 cut(s) 963
BspQI GCTCTTC 2 cut(s) 402, 443
BspT107I GGYRCC 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 1003
BsrI ACTGG 6 cut(s) 47, 161, 428, 455, 779, 1026
BssECI CCNNGG 3 cut(s) 31, 66, 580
BssMI GATC 4 cut(s) 261, 549, 955, 960
BssT1I CCWWGG 1 cut(s) 580
Bst2UI CCWGG 2 cut(s) 32, 68
Bst6I CTCTTC 2 cut(s) 402, 443
BstAPI GCANNNNNTGC 1 cut(s) 797
BstDEI CTNAG 3 cut(s) 9, 107, 120
BstF5I GGATG 1 cut(s) 90
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 4 cut(s) 264, 552, 958, 963
BstMAI GTCTC 4 cut(s) 423, 503, 689, 813
BstMBI GATC 4 cut(s) 261, 549, 955, 960
BstMWI GCNNNNNNNGC 1 cut(s) 797
BstNI CCWGG 2 cut(s) 32, 68
BstNSI RCATGY 2 cut(s) 436, 613
BstSCI CCNGG 2 cut(s) 30, 66
BstSFI CTRYAG 1 cut(s) 789
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 3 cut(s) 315, 522, 986
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuRI GGCC 4 cut(s) 112, 350, 387, 484
BtsCI GGATG 1 cut(s) 90
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 2 cut(s) 168, 879
BveI ACCTGC 1 cut(s) 796
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 933, 944, 1054
Csp6I GTAC 2 cut(s) 626, 776
CviAII CATG 5 cut(s) 203, 269, 433, 610, 937
CviQI GTAC 2 cut(s) 626, 776
DdeI CTNAG 3 cut(s) 9, 107, 120
DpnI GATC 4 cut(s) 263, 551, 957, 962
DpnII GATC 4 cut(s) 261, 549, 955, 960
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 402, 443
EarI CTCTTC 2 cut(s) 402, 443
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 580
Eco147I AGGCCT 2 cut(s) 112, 350
Eco31I GGTCTC 1 cut(s) 689
Eco32I GATATC 1 cut(s) 104
Eco47I GGWCC 3 cut(s) 933, 944, 1054
Eco57I CTGAAG 1 cut(s) 135
EcoRII CCWGG 2 cut(s) 30, 66
EcoRV GATATC 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 5 cut(s) 206, 272, 436, 613, 940
FaqI GGGAC 1 cut(s) 946
FatI CATG 5 cut(s) 202, 268, 432, 609, 936
FauI CCCGC 1 cut(s) 102
FbaI TGATCA 2 cut(s) 261, 960
Fnu4HI GCNGC 4 cut(s) 329, 536, 909, 975
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 4 cut(s) 329, 536, 909, 975
FspBI CTAG 2 cut(s) 573, 581
GlaI GCGC 1 cut(s) 98
GluI GCNGC 4 cut(s) 329, 536, 909, 975
HaeIII GGCC 4 cut(s) 112, 350, 387, 484
HapII CCGG 1 cut(s) 953
HhaI GCGC 1 cut(s) 99
Hin1II CATG 5 cut(s) 206, 272, 436, 613, 940
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 6 cut(s) 146, 190, 367, 469, 585, 715
HpaII CCGG 1 cut(s) 953
HphI GGTGA 1 cut(s) 889
Hpy166II GTNNAC 4 cut(s) 662, 811, 880, 1031
Hpy188I TCNGA 4 cut(s) 280, 406, 700, 960
Hpy188III TCNNGA 1 cut(s) 757
Hpy8I GTNNAC 4 cut(s) 662, 811, 880, 1031
HpyAV CCTTC 6 cut(s) 79, 340, 371, 452, 500, 883
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 6 cut(s) 356, 538, 727, 791, 800, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 797
HpyF3I CTNAG 3 cut(s) 9, 107, 120
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 5 cut(s) 206, 272, 436, 613, 940
HspAI GCGC 1 cut(s) 97
KpnI GGTACC 1 cut(s) 779
Ksp22I TGATCA 2 cut(s) 261, 960
Kzo9I GATC 4 cut(s) 261, 549, 955, 960
LguI GCTCTTC 2 cut(s) 402, 443
LmnI GCTCC 2 cut(s) 532, 845
Lsp1109I GCAGC 3 cut(s) 315, 522, 986
LweI GCATC 1 cut(s) 691
MaeI CTAG 2 cut(s) 573, 581
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 2 cut(s) 575, 1021
MalI GATC 4 cut(s) 263, 551, 957, 962
MboI GATC 4 cut(s) 261, 549, 955, 960
MboII GAAGA 5 cut(s) 293, 313, 313, 419, 430
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 1 cut(s) 867
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 5 cut(s) 132, 156, 212, 589, 903
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 4 cut(s) 140, 463, 594, 709
MnlI CCTC 2 cut(s) 136, 994
Mox20I TGGCCA 1 cut(s) 387
MscI TGGCCA 1 cut(s) 387
MseI TTAA 4 cut(s) 545, 732, 1011, 1084
MslI CAYNNNNRTG 2 cut(s) 207, 699
Msp20I TGGCCA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 1 cut(s) 953
MspR9I CCNGG 2 cut(s) 32, 68
MunI CAATTG 1 cut(s) 903
Mva1269I GAATGC 3 cut(s) 800, 911, 976
MvaI CCWGG 2 cut(s) 32, 68
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 1 cut(s) 797
NdeII GATC 4 cut(s) 261, 549, 955, 960
NlaIII CATG 5 cut(s) 206, 272, 436, 613, 940
NlaIV GGNNCC 2 cut(s) 777, 934
NmuCI GTSAC 1 cut(s) 1021
NspI RCATGY 2 cut(s) 436, 613
PceI AGGCCT 2 cut(s) 112, 350
PciSI GCTCTTC 2 cut(s) 402, 443
PctI GAATGC 3 cut(s) 800, 911, 976
PfeI GAWTC 2 cut(s) 190, 367
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 4 cut(s) 330, 537, 910, 976
PleI GAGTC 4 cut(s) 140, 463, 593, 709
PpsI GAGTC 4 cut(s) 140, 463, 593, 709
Psp6I CCWGG 2 cut(s) 30, 66
PspGI CCWGG 2 cut(s) 30, 66
PspN4I GGNNCC 2 cut(s) 777, 934
PspPI GGNCC 3 cut(s) 933, 944, 1054
PstI CTGCAG 1 cut(s) 793
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 955
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 2 cut(s) 627, 777
RsaNI GTAC 2 cut(s) 626, 776
RseI CAYNNNNRTG 2 cut(s) 207, 699
SapI GCTCTTC 2 cut(s) 402, 443
SaqAI TTAA 4 cut(s) 545, 732, 1011, 1084
SatI GCNGC 4 cut(s) 329, 536, 909, 975
Sau3AI GATC 4 cut(s) 261, 549, 955, 960
Sau96I GGNCC 3 cut(s) 933, 944, 1054
SchI GAGTC 4 cut(s) 140, 463, 594, 709
ScrFI CCNGG 2 cut(s) 32, 68
SduI GDGCHC 1 cut(s) 867
SfaNI GCATC 1 cut(s) 691
SfcI CTRYAG 1 cut(s) 789
SinI GGWCC 3 cut(s) 933, 944, 1054
SmiMI CAYNNNNRTG 2 cut(s) 207, 699
Sse9I AATT 5 cut(s) 132, 156, 212, 589, 903
SseBI AGGCCT 2 cut(s) 112, 350
SsiI CCGC 3 cut(s) 95, 460, 908
SspI AATATT 1 cut(s) 928
SspMI CTAG 2 cut(s) 573, 581
StuI AGGCCT 2 cut(s) 112, 350
StyD4I CCNGG 2 cut(s) 30, 66
StyI CCWWGG 1 cut(s) 580
TaiI ACGT 1 cut(s) 816
TaqI TCGA 1 cut(s) 375
TaqII GACCGA 1 cut(s) 671
TasI AATT 5 cut(s) 132, 156, 212, 589, 903
TatI WGTACW 1 cut(s) 625
TauI GCSGC 1 cut(s) 911
TfiI GAWTC 2 cut(s) 190, 367
Tru1I TTAA 4 cut(s) 545, 732, 1011, 1084
Tru9I TTAA 4 cut(s) 545, 732, 1011, 1084
TscAI CASTG 2 cut(s) 168, 879
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 3 cut(s) 328, 535, 974
Tsp45I GTSAC 1 cut(s) 1021
TspDTI ATGAA 6 cut(s) 215, 225, 314, 656, 750, 793
TspRI CASTG 2 cut(s) 168, 879
Van91I CCANNNNNTGG 1 cut(s) 929
VpaK11BI GGWCC 3 cut(s) 933, 944, 1054
XapI RAATTY 2 cut(s) 132, 156
XceI RCATGY 2 cut(s) 436, 613
XmaJI CCTAGG 1 cut(s) 580
XspI CTAG 2 cut(s) 573, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.