MD06G1038700.v1.1

S-norcoclaurine synthase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016346
Physical Location & Seq
Reverse (-)
4976436 .. 4979077
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1038700.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGAAACTGATCAGGTAGTATTCCGCAGAAAAGACTTGGGCGGTTCTCTACCAGTTGAGAATGTTCAAGCTCTTGCTTCCAGTACCTTAAAAGAAATACCACCCCGATACATCCGGCCTGAAATCCAACATGAGCCAGCTTCCGTTGAGGACTCGCTTCAAATTCCAGTTGTCGATACGAACAAGCTTAATGATGATGACGATGATGAATTAGCCAAACTTCATTTGGCTTGTAAGGAATGGGGTTTCTTTCAGTTGATCAATCATGATGTACCAGAAGAAGTGATTAAGAAAATGAAGAGTGACACCGAGGAGTTCTTCCAGCTGCCACTAGAAGTAAAGAAGGCTTATGCACAGCTGCCAAATCATATTGAAGGTTATGGCCAAGCCTTTGTAGTTTCTGAAGACCAAAAACTTGACTGGGGAGACATGCTCTTCCTTCTCTCACAACCACTCTCCCTCAGAAATCTCACATTCTGGCCTACCCTCCCCACTTCTTTCAGGGAGACACTGGATAAGTACTCAGAGGAACTTCAACAAGTCACACTCTATATATGGAAGTTGATATGTAAGAACCTGGGGCTCAATTCAGAGGAGATGGAAAGCATGTTCGAGGATGGAACGCAAGGTCTAAGGATGAATTATTATCCACCCTGTCAGCAAGCTAGCAGGGTTATGGGTCTCACTCCACATTCAGATGCTGTGGGTTTGACTCTATTAGTTCAAGTTAACGAAGTTCAAGGTTTACAAATCAAGAAAAATGGCAGATGGGTACCCGTTAAACCGGTACCCAGTGCATTCATCGTCAACATTGGCGACATCATTGAGATAATGAGTAATGGAGAGTATAAGAGCATAGAGCACAGAGCTGTGGTGAACACAGAAAGGGAACGGCTTTCAATTGCAGCATTTCACAGCCCAAATCTGAGGACCGCAATTGGTCCCTTGCCAGATCTTGTCAAGGATAATGCAGCAAACTACAAGACTGTAAGCAGCGAGGAGTATATTAAACTCGTCGTAACCAGCAAACTCGACGGTAAAAACCTACTGGAACATATGAAATTGAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

358

Amino Acids

40.56

Weight (kDa)

5.13

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 162 2.6e-31 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 209 - 307 9.4e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21360
malus_domestica MD06G1038700.v1.1
prunus_persica Prupe.5G043900_v2.0.a1 Prupe.5G043900_v2.0.a1
pyrus_communis pycom06g03250
rosa_chinensis RchiOBHm_Chr7g0208701
rosa_laevigata RLG00000003198 RLG00000003200
rosa_multiflora Rmu_sc0004103.1_g000007
rosa_roxburghii Rroxscaffold_3G00250300
rosa_rugosa Rorug07G0105500
rosa_samantha Rh7AG240500 Rh7CG255100 Rh7DG246500
rosa_wichuraiana Rw7G020420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 772, 787
AccB1I GGYRCC 2 cut(s) 772, 787
AciI CCGC 3 cut(s) 25, 42, 933
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 5 cut(s) 85, 273, 521, 774, 789
AgeI ACCGGT 1 cut(s) 784
AgsI TTSAA 8 cut(s) 68, 161, 374, 536, 725, 740, 900, 1066
AjnI CCWGG 1 cut(s) 576
AloI GAACNNNNNNTCC 2 cut(s) 593, 625
AluBI AGCT 7 cut(s) 71, 140, 187, 325, 358, 665, 869
AluI AGCT 7 cut(s) 71, 140, 187, 325, 358, 665, 869
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 3 cut(s) 420, 500, 686
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 3 cut(s) 116, 382, 479
ApeKI GCWGC 5 cut(s) 325, 358, 905, 971, 993
ApoI RAATTY 1 cut(s) 162
AsiGI ACCGGT 1 cut(s) 784
Asp718I GGTACC 2 cut(s) 772, 787
AspS9I GGNCC 2 cut(s) 930, 941
AsuHPI GGTGA 1 cut(s) 886
AsuNHI GCTAGC 1 cut(s) 665
AvaII GGWCC 2 cut(s) 930, 941
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 2 cut(s) 772, 787
BanII GRGCYC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 5 cut(s) 312, 345, 917, 983, 1005
BccI CCATC 3 cut(s) 592, 611, 762
BceAI ACGGC 1 cut(s) 908
BciT130I CCWGG 1 cut(s) 578
BclI TGATCA 2 cut(s) 10, 258
BcoDI GTCTC 3 cut(s) 420, 500, 686
BfaI CTAG 2 cut(s) 332, 666
BglII AGATCT 1 cut(s) 952
BisI GCNGC 5 cut(s) 326, 359, 906, 972, 994
BlsI GCNGC 5 cut(s) 327, 360, 907, 973, 995
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 1 cut(s) 578
Bme18I GGWCC 2 cut(s) 930, 941
BmgT120I GGNCC 2 cut(s) 930, 941
BmiI GGNNCC 3 cut(s) 774, 789, 943
BmrFI CCNGG 1 cut(s) 578
BmrI ACTGGG 2 cut(s) 430, 786
BmsI GCATC 1 cut(s) 688
BmtI GCTAGC 1 cut(s) 669
BmuI ACTGGG 2 cut(s) 430, 786
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 417, 449
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 309, 577
BsaWI WCCGGW 1 cut(s) 784
Bse118I RCCGGY 1 cut(s) 784
Bse1I ACTGG 7 cut(s) 53, 81, 167, 425, 516, 792, 1053
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 2 cut(s) 309, 577
BseGI GGATG 3 cut(s) 111, 622, 642
BseMII CTCAG 3 cut(s) 475, 537, 917
BseNI ACTGG 7 cut(s) 53, 81, 167, 425, 516, 792, 1053
BseRI GAGGAG 3 cut(s) 326, 608, 1013
BseXI GCAGC 5 cut(s) 312, 345, 917, 983, 1005
BshFI GGCC 3 cut(s) 118, 384, 481
BshNI GGYRCC 2 cut(s) 772, 787
BshTI ACCGGT 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 864
BsiSI CCGG 2 cut(s) 115, 785
BslFI GGGAC 1 cut(s) 927
BsmAI GTCTC 3 cut(s) 420, 500, 686
BsmFI GGGAC 1 cut(s) 927
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 3 cut(s) 118, 384, 481
Bso31I GGTCTC 1 cut(s) 686
Bsp1286I GDGCHC 2 cut(s) 585, 864
Bsp143I GATC 3 cut(s) 10, 258, 952
BspACI CCGC 3 cut(s) 25, 42, 933
BspANI GGCC 3 cut(s) 118, 384, 481
BspCNI CTCAG 3 cut(s) 474, 536, 918
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 774, 789, 943
BspOI GCTAGC 1 cut(s) 669
BspQI GCTCTTC 1 cut(s) 440
BspT107I GGYRCC 2 cut(s) 772, 787
BspTNI GGTCTC 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 784
BsrI ACTGG 7 cut(s) 53, 81, 167, 425, 516, 792, 1053
BssAI RCCGGY 1 cut(s) 784
BssECI CCNNGG 2 cut(s) 309, 577
BssMI GATC 3 cut(s) 10, 258, 952
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 988, 1037
Bst6I CTCTTC 2 cut(s) 293, 440
BstC8I GCNNGC 3 cut(s) 138, 663, 667
BstDEI CTNAG 4 cut(s) 461, 523, 632, 926
BstF5I GGATG 3 cut(s) 111, 622, 642
BstKTI GATC 3 cut(s) 13, 261, 955
BstMAI GTCTC 3 cut(s) 420, 500, 686
BstMBI GATC 3 cut(s) 10, 258, 952
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 2 cut(s) 433, 610
BstSCI CCNGG 1 cut(s) 576
BstV1I GCAGC 5 cut(s) 312, 345, 917, 983, 1005
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 952
BstYI RGATCY 1 cut(s) 952
BsuRI GGCC 3 cut(s) 118, 384, 481
BtsCI GGATG 3 cut(s) 111, 622, 642
BtsIMutI CAGTG 2 cut(s) 509, 799
Cac8I GCNNGC 3 cut(s) 138, 663, 667
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 784
Cfr13I GGNCC 2 cut(s) 930, 941
Csp6I GTAC 5 cut(s) 84, 272, 520, 773, 788
CspAI ACCGGT 1 cut(s) 784
CviAII CATG 4 cut(s) 131, 266, 430, 607
CviQI GTAC 5 cut(s) 84, 272, 520, 773, 788
DdeI CTNAG 4 cut(s) 461, 523, 632, 926
DpnI GATC 3 cut(s) 12, 260, 954
DpnII GATC 3 cut(s) 10, 258, 952
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 293, 440
EarI CTCTTC 2 cut(s) 293, 440
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 2 cut(s) 930, 941
Eco57I CTGAAG 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 576
EcoT38I GRGCYC 1 cut(s) 585
FaeI CATG 4 cut(s) 134, 269, 433, 610
FaqI GGGAC 1 cut(s) 927
FatI CATG 4 cut(s) 130, 265, 429, 606
FauNDI CATATG 1 cut(s) 1056
FbaI TGATCA 2 cut(s) 10, 258
Fnu4HI GCNGC 5 cut(s) 326, 359, 906, 972, 994
FokI GGATG 3 cut(s) 98, 629, 649
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 5 cut(s) 326, 359, 906, 972, 994
FspBI CTAG 2 cut(s) 332, 666
GluI GCNGC 5 cut(s) 326, 359, 906, 972, 994
HaeIII GGCC 3 cut(s) 118, 384, 481
HapII CCGG 2 cut(s) 115, 785
Hin1II CATG 4 cut(s) 134, 269, 433, 610
HincII GTYRAC 2 cut(s) 730, 808
HindII GTYRAC 2 cut(s) 730, 808
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 2 cut(s) 152, 712
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 2 cut(s) 115, 785
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 4 cut(s) 730, 746, 808, 877
Hpy188I TCNGA 6 cut(s) 403, 464, 526, 592, 697, 927
Hpy188III TCNNGA 2 cut(s) 266, 754
Hpy8I GTNNAC 4 cut(s) 730, 746, 808, 877
Hpy99I CGWCG 2 cut(s) 1019, 1037
HpyAV CCTTC 4 cut(s) 337, 368, 449, 1060
HpyCH4III ACNGT 2 cut(s) 988, 1037
HpyCH4V TGCA 4 cut(s) 353, 797, 905, 971
HpyF3I CTNAG 4 cut(s) 461, 523, 632, 926
Hsp92II CATG 4 cut(s) 134, 269, 433, 610
KpnI GGTACC 2 cut(s) 776, 791
Ksp22I TGATCA 2 cut(s) 10, 258
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 3 cut(s) 10, 258, 952
LguI GCTCTTC 1 cut(s) 440
Lsp1109I GCAGC 5 cut(s) 312, 345, 917, 983, 1005
LweI GCATC 1 cut(s) 688
MaeI CTAG 2 cut(s) 332, 666
MaeIII GTNAC 3 cut(s) 302, 541, 1018
MalI GATC 3 cut(s) 12, 260, 954
MboI GATC 3 cut(s) 10, 258, 952
MboII GAAGA 5 cut(s) 290, 310, 310, 416, 427
MfeI CAATTG 2 cut(s) 900, 936
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 2 cut(s) 585, 864
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 7 cut(s) 162, 209, 586, 640, 900, 936, 1061
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 2 cut(s) 146, 706
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 9 cut(s) 142, 304, 470, 497, 520, 586, 607, 921, 991
Mox20I TGGCCA 1 cut(s) 384
MscI TGGCCA 1 cut(s) 384
MseI TTAA 6 cut(s) 89, 189, 288, 729, 780, 1008
MslI CAYNNNNRTG 1 cut(s) 696
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 2 cut(s) 325, 358
MspI CCGG 2 cut(s) 115, 785
MspR9I CCNGG 1 cut(s) 578
MunI CAATTG 2 cut(s) 900, 936
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 1 cut(s) 578
NdeI CATATG 1 cut(s) 1056
NdeII GATC 3 cut(s) 10, 258, 952
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 4 cut(s) 134, 269, 433, 610
NlaIV GGNNCC 3 cut(s) 774, 789, 943
NmuCI GTSAC 2 cut(s) 302, 541
NspI RCATGY 2 cut(s) 433, 610
PagI TCATGA 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 440
PctI GAATGC 1 cut(s) 797
PinAI ACCGGT 1 cut(s) 784
PkrI GCNGC 5 cut(s) 327, 360, 907, 973, 995
PleI GAGTC 2 cut(s) 146, 706
PpsI GAGTC 2 cut(s) 146, 706
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 3 cut(s) 774, 789, 943
PspPI GGNCC 2 cut(s) 930, 941
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 952
PvuII CAGCTG 2 cut(s) 325, 358
RsaI GTAC 5 cut(s) 85, 273, 521, 774, 789
RsaNI GTAC 5 cut(s) 84, 272, 520, 773, 788
RseI CAYNNNNRTG 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 440
SaqAI TTAA 6 cut(s) 89, 189, 288, 729, 780, 1008
SatI GCNGC 5 cut(s) 326, 359, 906, 972, 994
Sau3AI GATC 3 cut(s) 10, 258, 952
Sau96I GGNCC 2 cut(s) 930, 941
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 2 cut(s) 146, 706
ScrFI CCNGG 1 cut(s) 578
SduI GDGCHC 2 cut(s) 585, 864
SfaNI GCATC 1 cut(s) 688
SinI GGWCC 2 cut(s) 930, 941
SmiMI CAYNNNNRTG 1 cut(s) 696
Sse9I AATT 7 cut(s) 162, 209, 586, 640, 900, 936, 1061
SsiI CCGC 3 cut(s) 25, 42, 933
SspMI CTAG 2 cut(s) 332, 666
StyD4I CCNGG 1 cut(s) 576
TaaI ACNGT 2 cut(s) 988, 1037
TaqI TCGA 3 cut(s) 174, 612, 1032
TasI AATT 7 cut(s) 162, 209, 586, 640, 900, 936, 1061
TatI WGTACW 1 cut(s) 519
Tru1I TTAA 6 cut(s) 89, 189, 288, 729, 780, 1008
Tru9I TTAA 6 cut(s) 89, 189, 288, 729, 780, 1008
TscAI CASTG 2 cut(s) 516, 799
TseFI GTSAC 2 cut(s) 302, 541
TseI GCWGC 5 cut(s) 325, 358, 905, 971, 993
Tsp45I GTSAC 2 cut(s) 302, 541
TspDTI ATGAA 6 cut(s) 212, 222, 311, 653, 790, 1073
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 2 cut(s) 516, 799
VpaK11BI GGWCC 2 cut(s) 930, 941
XapI RAATTY 1 cut(s) 162
XceI RCATGY 2 cut(s) 433, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XspI CTAG 2 cut(s) 332, 666
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.