pycom06g03250

S-norcoclaurine synthase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0016346
Physical Location & Seq
Reverse (-)
4048941 .. 4050604
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g03250.4

Sequence Viewer

Length: 1077 bp
ATGGAAACTGATGAGGTACTATTCAGCAGAAAAGACTTGGGCGGTTCTCTACCAGTTGAGAATGTTCAAGCTCTTGCGTCCAACACCTTGAAAGAAATACCACCCCGATACATCCGGCCTGAAATCGAACATGAGCCAGCTTCCGTTGAGGACTCGCTTCAAATTCCAGTTGTTGATATGAACAAGCTTAATGATGATGATGATGATGATGAATTAGCTAAACTTCATTTGGCTTGTAAGGAATGGGGTTTCTTTCAGTTGATCAATCATGATGTACCAGAAGAAGTGATTAAGAAAATGAAGAGTGACACTGAGGAGTTCTTCCAGCTGCCACTAGAAGTAAAGAAGACTTATGCACAGCTGCCAAATCATATTGAAGGTTATGGCCAAGCCTTTGTAGTTTCTGAAGACCAAAAACTTGACTGGGGAGACATGCTCTTCCTACTGTCACAACCACTCTCCGTCAGAAATCTCACATTCTGGCCTACCCTCCCCACTTCTTTCAGGGAGACACTGAATAAGTACTCGGAGGAACTTCAACAAATCCCACTCTATCTATGGAAGTTGATAAGTAAGAATCTGGGGCTCGATTCAGAGGAGCTGGCAAGCATGTTCGAGGATGGAACGCAAGGTCTAAGGATGAATTATTATCCACCCTGTCAGCAAGCTAGCAGGGTTATGGGTCTCACTCCACATTCAGATTCTGTGGGTTTGACTCTATTAGTTCAAGTTAACGAAGTTCAAGGTTTACAAATCAAGAAAAATGGCAGATGGGTACCCGTTAAACCGGTACCCGGTGCATTCATCGTCAACATTGGCGACATCATTGAGATAATGAGTAATGGAGAGTATAAGAGCATAGAGCACAGAGCTGTGGTGAACACAGAAAGGGAACGGCTTTCAATTGCAGCGTTTCACAGCCCGAATATGAGGACCGCGATTGGTCCCTTGCCCGATCTTGTCAAGGATAATGCAGCAAACTACAAGACCGTAAGCAGCGAGGAGTATATTAAACACGTCGTAACCAGCAAACTCGACGGTAAAAACCTACTGGAACATATGAAATTGAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

359

Amino Acids

40.64

Weight (kDa)

5.02

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21360
malus_domestica MD06G1038700.v1.1
prunus_persica Prupe.5G043900_v2.0.a1 Prupe.5G043900_v2.0.a1
pyrus_communis pycom06g03250
rosa_chinensis RchiOBHm_Chr7g0208701
rosa_laevigata RLG00000003198 RLG00000003200
rosa_multiflora Rmu_sc0004103.1_g000007
rosa_roxburghii Rroxscaffold_3G00250300
rosa_rugosa Rorug07G0105500
rosa_samantha Rh7AG240500 Rh7CG255100 Rh7DG246500
rosa_wichuraiana Rw7G020420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 775, 790
AccB1I GGYRCC 2 cut(s) 775, 790
AccII CGCG 1 cut(s) 938
AciI CCGC 2 cut(s) 42, 936
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 5 cut(s) 18, 276, 524, 777, 792
AfiI CCNNNNNNNGG 1 cut(s) 794
AflIII ACRYGT 1 cut(s) 1015
AgeI ACCGGT 1 cut(s) 787
AgsI TTSAA 9 cut(s) 68, 91, 161, 377, 539, 728, 743, 903, 1069
AjiI CACGTC 1 cut(s) 1018
AluBI AGCT 9 cut(s) 71, 140, 187, 218, 328, 361, 601, 668, 872
AluI AGCT 9 cut(s) 71, 140, 187, 218, 328, 361, 601, 668, 872
Alw21I GWGCWC 1 cut(s) 867
Alw26I GTCTC 3 cut(s) 423, 503, 689
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 3 cut(s) 116, 385, 482
ApeKI GCWGC 5 cut(s) 328, 361, 908, 974, 996
ApoI RAATTY 1 cut(s) 162
AsiGI ACCGGT 1 cut(s) 787
Asp718I GGTACC 2 cut(s) 775, 790
AspS9I GGNCC 2 cut(s) 933, 944
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 1 cut(s) 889
AsuNHI GCTAGC 1 cut(s) 668
AvaII GGWCC 2 cut(s) 933, 944
BalI TGGCCA 1 cut(s) 387
BanI GGYRCC 2 cut(s) 775, 790
BanII GRGCYC 1 cut(s) 588
BbsI GAAGAC 2 cut(s) 353, 414
Bbv12I GWGCWC 1 cut(s) 867
BbvI GCAGC 5 cut(s) 315, 348, 920, 986, 1008
BccI CCATC 2 cut(s) 614, 765
BceAI ACGGC 1 cut(s) 911
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 3 cut(s) 423, 503, 689
BfaI CTAG 2 cut(s) 335, 669
BisI GCNGC 5 cut(s) 329, 362, 909, 975, 997
BlsI GCNGC 5 cut(s) 330, 363, 910, 976, 998
BmcAI AGTACT 1 cut(s) 524
Bme1390I CCNGG 1 cut(s) 795
Bme18I GGWCC 2 cut(s) 933, 944
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 2 cut(s) 933, 944
BmiI GGNNCC 3 cut(s) 777, 792, 946
BmrFI CCNGG 1 cut(s) 795
BmrI ACTGGG 1 cut(s) 433
BmtI GCTAGC 1 cut(s) 672
BmuI ACTGGG 1 cut(s) 433
BpiI GAAGAC 2 cut(s) 353, 414
BplI GAGNNNNNCTC 2 cut(s) 420, 452
BpuMI CCSGG 1 cut(s) 795
BsaI GGTCTC 1 cut(s) 689
BsaWI WCCGGW 1 cut(s) 787
Bsc4I CCNNNNNNNGG 1 cut(s) 794
Bse118I RCCGGY 1 cut(s) 787
Bse1I ACTGG 4 cut(s) 53, 167, 428, 1056
BseGI GGATG 3 cut(s) 111, 625, 645
BseLI CCNNNNNNNGG 1 cut(s) 794
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 4 cut(s) 53, 167, 428, 1056
BseRI GAGGAG 3 cut(s) 329, 611, 1016
BseXI GCAGC 5 cut(s) 315, 348, 920, 986, 1008
Bsh1236I CGCG 1 cut(s) 938
BshFI GGCC 3 cut(s) 118, 387, 484
BshNI GGYRCC 2 cut(s) 775, 790
BshTI ACCGGT 1 cut(s) 787
BsiHKAI GWGCWC 1 cut(s) 867
BsiSI CCGG 3 cut(s) 115, 788, 795
BslFI GGGAC 1 cut(s) 930
BslI CCNNNNNNNGG 1 cut(s) 794
BsmAI GTCTC 3 cut(s) 423, 503, 689
BsmFI GGGAC 1 cut(s) 930
BsmI GAATGC 1 cut(s) 800
BsnI GGCC 3 cut(s) 118, 387, 484
Bso31I GGTCTC 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 588, 867
Bsp143I GATC 2 cut(s) 261, 955
BspACI CCGC 2 cut(s) 42, 936
BspANI GGCC 3 cut(s) 118, 387, 484
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 1 cut(s) 938
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 777, 792, 946
BspOI GCTAGC 1 cut(s) 672
BspQI GCTCTTC 1 cut(s) 443
BspT107I GGYRCC 2 cut(s) 775, 790
BspTNI GGTCTC 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 787
BsrI ACTGG 4 cut(s) 53, 167, 428, 1056
BssAI RCCGGY 1 cut(s) 787
BssMI GATC 2 cut(s) 261, 955
Bst4CI ACNGT 3 cut(s) 447, 991, 1040
Bst6I CTCTTC 2 cut(s) 296, 443
BstC8I GCNNGC 5 cut(s) 138, 603, 607, 666, 670
BstDEI CTNAG 2 cut(s) 312, 635
BstF5I GGATG 3 cut(s) 111, 625, 645
BstFNI CGCG 1 cut(s) 938
BstKTI GATC 2 cut(s) 264, 958
BstMAI GTCTC 3 cut(s) 423, 503, 689
BstMBI GATC 2 cut(s) 261, 955
BstNSI RCATGY 2 cut(s) 436, 613
BstSCI CCNGG 1 cut(s) 793
BstUI CGCG 1 cut(s) 938
BstV1I GCAGC 5 cut(s) 315, 348, 920, 986, 1008
BstV2I GAAGAC 2 cut(s) 353, 414
BsuRI GGCC 3 cut(s) 118, 387, 484
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 3 cut(s) 111, 625, 645
BtsIMutI CAGTG 2 cut(s) 309, 512
Cac8I GCNNGC 5 cut(s) 138, 603, 607, 666, 670
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 787
Cfr13I GGNCC 2 cut(s) 933, 944
CseI GACGC 1 cut(s) 66
Csp6I GTAC 5 cut(s) 17, 275, 523, 776, 791
CspAI ACCGGT 1 cut(s) 787
CviAII CATG 4 cut(s) 131, 269, 433, 610
CviQI GTAC 5 cut(s) 17, 275, 523, 776, 791
DdeI CTNAG 2 cut(s) 312, 635
DpnI GATC 2 cut(s) 263, 957
DpnII GATC 2 cut(s) 261, 955
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 296, 443
EarI CTCTTC 2 cut(s) 296, 443
Eco24I GRGCYC 1 cut(s) 588
Eco31I GGTCTC 1 cut(s) 689
Eco47I GGWCC 2 cut(s) 933, 944
Eco57I CTGAAG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 588
FaeI CATG 4 cut(s) 134, 272, 436, 613
FaqI GGGAC 1 cut(s) 930
FatI CATG 4 cut(s) 130, 268, 432, 609
FauNDI CATATG 1 cut(s) 1059
FbaI TGATCA 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 329, 362, 909, 975, 997
FokI GGATG 3 cut(s) 98, 632, 652
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 5 cut(s) 329, 362, 909, 975, 997
FspBI CTAG 2 cut(s) 335, 669
GluI GCNGC 5 cut(s) 329, 362, 909, 975, 997
HaeIII GGCC 3 cut(s) 118, 387, 484
HapII CCGG 3 cut(s) 115, 788, 795
HgaI GACGC 1 cut(s) 66
Hin1II CATG 4 cut(s) 134, 272, 436, 613
HincII GTYRAC 2 cut(s) 733, 811
HindII GTYRAC 2 cut(s) 733, 811
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 5 cut(s) 152, 577, 590, 701, 715
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 3 cut(s) 115, 788, 795
HphI GGTGA 1 cut(s) 889
Hpy166II GTNNAC 4 cut(s) 733, 749, 811, 880
Hpy188I TCNGA 5 cut(s) 406, 467, 529, 595, 700
Hpy188III TCNNGA 2 cut(s) 269, 757
Hpy8I GTNNAC 4 cut(s) 733, 749, 811, 880
Hpy99I CGWCG 2 cut(s) 1022, 1040
HpyAV CCTTC 2 cut(s) 371, 1063
HpyCH4III ACNGT 3 cut(s) 447, 991, 1040
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 4 cut(s) 356, 800, 908, 974
HpyF3I CTNAG 2 cut(s) 312, 635
HpySE526I ACGT 1 cut(s) 1017
Hsp92II CATG 4 cut(s) 134, 272, 436, 613
KpnI GGTACC 2 cut(s) 779, 794
Ksp22I TGATCA 1 cut(s) 261
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 2 cut(s) 261, 955
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 1 cut(s) 598
Lsp1109I GCAGC 5 cut(s) 315, 348, 920, 986, 1008
MaeI CTAG 2 cut(s) 335, 669
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 3 cut(s) 305, 447, 1021
MalI GATC 2 cut(s) 263, 957
MboI GATC 2 cut(s) 261, 955
MboII GAAGA 6 cut(s) 293, 313, 313, 358, 419, 430
MfeI CAATTG 1 cut(s) 903
MhlI GDGCHC 2 cut(s) 588, 867
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 5 cut(s) 162, 212, 643, 903, 1064
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 2 cut(s) 146, 709
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 9 cut(s) 7, 142, 307, 500, 523, 589, 610, 924, 994
Mox20I TGGCCA 1 cut(s) 387
MscI TGGCCA 1 cut(s) 387
MseI TTAA 5 cut(s) 189, 291, 732, 783, 1011
Msp20I TGGCCA 1 cut(s) 387
MspA1I CMGCKG 2 cut(s) 328, 361
MspI CCGG 3 cut(s) 115, 788, 795
MspR9I CCNGG 1 cut(s) 795
MunI CAATTG 1 cut(s) 903
Mva1269I GAATGC 1 cut(s) 800
MvnI CGCG 1 cut(s) 938
NciI CCSGG 1 cut(s) 795
NdeI CATATG 1 cut(s) 1059
NdeII GATC 2 cut(s) 261, 955
NheI GCTAGC 1 cut(s) 668
NlaIII CATG 4 cut(s) 134, 272, 436, 613
NlaIV GGNNCC 3 cut(s) 777, 792, 946
NmuCI GTSAC 2 cut(s) 305, 447
NspI RCATGY 2 cut(s) 436, 613
PagI TCATGA 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 443
PctI GAATGC 1 cut(s) 800
PfeI GAWTC 3 cut(s) 577, 590, 701
PinAI ACCGGT 1 cut(s) 787
PkrI GCNGC 5 cut(s) 330, 363, 910, 976, 998
PleI GAGTC 2 cut(s) 146, 709
PpsI GAGTC 2 cut(s) 146, 709
PspN4I GGNNCC 3 cut(s) 777, 792, 946
PspPI GGNCC 2 cut(s) 933, 944
PstNI CAGNNNCTG 1 cut(s) 704
PvuII CAGCTG 2 cut(s) 328, 361
RsaI GTAC 5 cut(s) 18, 276, 524, 777, 792
RsaNI GTAC 5 cut(s) 17, 275, 523, 776, 791
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 5 cut(s) 189, 291, 732, 783, 1011
SatI GCNGC 5 cut(s) 329, 362, 909, 975, 997
Sau3AI GATC 2 cut(s) 261, 955
Sau96I GGNCC 2 cut(s) 933, 944
ScaI AGTACT 1 cut(s) 524
SchI GAGTC 2 cut(s) 146, 709
ScrFI CCNGG 1 cut(s) 795
SduI GDGCHC 2 cut(s) 588, 867
SinI GGWCC 2 cut(s) 933, 944
Sse9I AATT 5 cut(s) 162, 212, 643, 903, 1064
SsiI CCGC 2 cut(s) 42, 936
SspMI CTAG 2 cut(s) 335, 669
StyD4I CCNGG 1 cut(s) 793
TaaI ACNGT 3 cut(s) 447, 991, 1040
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 4 cut(s) 126, 588, 615, 1035
TasI AATT 5 cut(s) 162, 212, 643, 903, 1064
TatI WGTACW 1 cut(s) 522
TfiI GAWTC 3 cut(s) 577, 590, 701
Tru1I TTAA 5 cut(s) 189, 291, 732, 783, 1011
Tru9I TTAA 5 cut(s) 189, 291, 732, 783, 1011
TscAI CASTG 2 cut(s) 316, 519
TseFI GTSAC 2 cut(s) 305, 447
TseI GCWGC 5 cut(s) 328, 361, 908, 974, 996
Tsp45I GTSAC 2 cut(s) 305, 447
TspDTI ATGAA 7 cut(s) 194, 215, 225, 314, 656, 793, 1076
TspGWI ACGGA 2 cut(s) 133, 451
TspRI CASTG 2 cut(s) 316, 519
VpaK11BI GGWCC 2 cut(s) 933, 944
XapI RAATTY 1 cut(s) 162
XceI RCATGY 2 cut(s) 436, 613
XcmI CCANNNNNNNNNTGG 1 cut(s) 555
XspI CTAG 2 cut(s) 335, 669
ZrmI AGTACT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.