Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0002175

Physical Location & Seq

Genomic Coordinates Forward (+)

14681565 .. 14689399

Gene Structure 7835 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g23320.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGGAGCAAATGGAGGGACAGAGCTGGAGGGAACTGGTGAAGAATATGCTCCCGCCAGAACCGCTATCTGAATCACAAAGATTGGTTATCAATATGGGTCCACCGGTTATTGAACCAATTCCTCTGCCGGTGTCTCGTATTGCTGGAGTCACAAGTTCCCCTACTCAAAGTCCAAGGGTATCAGGAAGCTCAGAATCTGTAGTGTCGGTCTTGCAAAACCCTGACTTTTCTTCAGCATCACCTTCAGCCTCTCCAGGCTCAGTTCATAATCCTTCAAGTGTTCCACCCAAACAAGTTACTAATGAAGTGAAGCGAGCTCCAGTGGTCACTTTCAATACTGTTGATAGGTCTCAGAAAGATGTTGATGTAGAGAAGCCAGTTTTTACACCTTATGTTCCTGTTCCAAAGGACAAGAAGAAGAAGAAAAGTAGAGTTTGTTACCGGTGCCGAAAAGGAAAGTGGGAAACCAAAGAGTCGTGCCTGGTTTGTGATGCCAAGTATTGCAGCAATTGTGTGCTTAGGGCCATGGGTTCAATGCCTGAAGGGCGTAAGTGTGTGACATGCATCAGTCAGCCAATTGATGAATCAAAGCGGCTGAAATTGGGTAAACAGTCGAGGGTACTGTCCAGATTGCTCAGTCCCTTGGAAGTCAAGCAGATAATGAAAGCAGAGAAAGAATGTGCTGCCAATCAGCTACGGCCAGAGCAGCTGATCGTAAATGGTGTTCCGTTAAAACCCGAAGAGATGGCCGAATTACTTGGATGCCCTTTACCTCCGCGAAAGTTGAAACCTGGTAGATATTGGTATGACAAAGAATCTGGTCTCTGGGGAAAGGAGGGAGAAAAACCAGATAGAATCATAAGTTCAAATTTAAATTTTACTGGGAAACTTAGCTCTGAAGCAAGCAACGGAAACACTGAAGTTTATATCAATGGTCGAGAAATTACAAAACTTGAGTTGAGGGTGCTTAAGGTGGCAAATGTCCAATGTCCACGTGACACCCATTTTTGGGTCTATGATGATGGTCGTTATGAGGAAGAAGGTCAGAACAATATCAGGGGCAACATTTGGGAGAAGGCTACAACTCGGTTCGTCTGTAGCCTGTTCTCTTTACCCGTGCTGCATGGTCAACCTCATGGAACAAGAGATGAGGCCAGCAATTATACCACCATCCCAAATTATTTGGAGCAGAAAAAAACTCAGAAGCTTCTTCTTCTTGGCCTTCCAGGGTCTGGAACAAGCACTATCTTCAAGCAGGCGAAGTTCTTGTATGGGAACAAGTTTACTGCAGAAGAGGTGCAGGATATAAAGTTGATGATCCAAAGTAATATGTACAAATATCTAAGCATTCTGCTTGATGGAAGGGAGCGTTTTGAAGAGGAGGCTTTGGCTAGGATGGGTGCACATGGTTCCAATGATCAAACCAAAGCAACAGGTAGTGAGGTTGATAGTGATGAAACTAGCCAATGTATCTACTCCATCAACCCAAGATTGAAGCATTTCTCTGACTGGCTTCTGGACATCATTGCTACTGGAGATTTAGATGCATTTTTCCCTGCTGCAACACGCGAATATGCTCCATTAGTTGAAGAGGTGTGGAAGGATCCTGCTATTCAGGAAACATATAAAAGAAAAGATGAGCTTCACTTCCTCCCAGAGGTTGCAGAATACTTTTTAAGCCAGGCTGTTGAGGTATCTAGCAATGAGTATGAACCTTCAGAACGAGACATTCTATATGCAGAAGGGGTCACTCAAGGAAATGGCTTGGCTTTCATAGAATTCTCTCTTGATGATCGAAGTCCGATGTCTGAAACCTATACAGAAAATCTGGATGCTCCGCCACCGCCACTAACCAAATACCAACTCATAAGAGTTAATGCCAAGGGGATGAATGAAGGGTGTAAGTGGGTGGAAATGTTTGAAGATGTGCGTGTGGTGGTGTTTTGTGTCGCTCTTAGTGATTATGATCAGGTGTGGATTTCCCCAGACAGTAGTGGGAGCGGATCATTGCTTCAAAATAAGATGATACAAAGCAAGGAGCTATTTGAGACTATGGTCAGACACCCTTGCTTTAAAGATACCCCTTTTGTTTTGGTACTAAACAAGTATGATCTCTTTGAGGACAAGGTCAATCAGATGCCTTTAAGTACTTGTGAATGGTTCTATGATTTTAGTCCAGTGAAGCCTCACCACAATAACCAGTCGCTGGCCCAGCAAGCTTACTTCTATGTTGCAATGAAGTTCAAAGACCTTTATGCTTCCATCACTGGTCGAAAACTTTTTGTGTGGCAGGCTCGGGCAAGGGACCGAGTGACAGTCGATGAGGGATTCAAGTACATAAGGGAGGTTCTCAGGTGGGATGAGGAGAAGGAGGCGACTTACTACGGCGGACCAGAGGATTCATTTTACAGTACAGATATGAGCTCCTCGCCATATGTCAGGCAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009723 GO:0009725 GO:0009898 GO:0009987 GO:0010033 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

817

Amino Acids

92.65

Weight (kDa)

5.78

Isoelectric Point (pI)

48.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	396 - 783	6.8e-75	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930
fragaria_vesca	FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320
malus_domestica	MD06G1025000.v1.1
prunus_persica	Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1
pyrus_communis	pycom06g02030
rosa_chinensis	RchiOBHm_Chr7g0212271
rosa_laevigata	RLG00000002900
rosa_multiflora	Rmu_sc0001911.1_g000047
rosa_roxburghii	Rroxscaffold_3G00246940
rosa_rugosa	Rorug07G0133000 Rorug07G0133100 Rorug07G0133200 Rorug07G0133200
rosa_samantha	Rh7AG265800 Rh7BG260000
rosa_wichuraiana	Rw7G022760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	444
AccB7I	CCANNNNNTGG	2 cut(s)	1232, 2206
AccBSI	CCGCTC	1 cut(s)	2001
AccII	CGCG	2 cut(s)	778, 1569
AciI	CCGC	8 cut(s)	53, 62, 592, 776, 1838, 1844, 2001, 2388
AclWI	GGATC	4 cut(s)	1312, 1598, 1611, 2011
AcoI	YGGCCR	2 cut(s)	698, 747
AcsI	RAATTY	3 cut(s)	868, 874, 1778
AcuI	CTGAAG	6 cut(s)	216, 228, 561, 918, 939, 1701
AcvI	CACGTG	1 cut(s)	995
AfaI	GTAC	6 cut(s)	621, 1334, 2097, 2149, 2336, 2413
AfiI	CCNNNNNNNGG	5 cut(s)	1008, 1009, 1232, 1657, 2206
AflII	CTTAAG	1 cut(s)	968
AgeI	ACCGGT	2 cut(s)	103, 441
AjnI	CCWGG	5 cut(s)	253, 480, 790, 1225, 1680
Alw21I	GWGCWC	3 cut(s)	319, 1405, 2426
Alw26I	GTCTC	5 cut(s)	138, 354, 827, 1719, 2042
Alw44I	GTGCAC	1 cut(s)	1401
AlwI	GGATC	4 cut(s)	1312, 1598, 1611, 2011
AlwNI	CAGNNNCTG	3 cut(s)	197, 1232, 2206
Ama87I	CYCGRG	1 cut(s)	2295
AoxI	GGCC	6 cut(s)	522, 698, 747, 1152, 1219, 2208
ApaLI	GTGCAC	1 cut(s)	1401
ApeKI	GCWGC	5 cut(s)	504, 683, 706, 1120, 1559
ApoI	RAATTY	3 cut(s)	868, 874, 1778
AsiGI	ACCGGT	2 cut(s)	103, 441
Asp700I	GAANNNNTTC	2 cut(s)	117, 1499
AspS9I	GGNCC	5 cut(s)	98, 522, 2209, 2305, 2390
AsuHPI	GGTGA	3 cut(s)	49, 231, 2180
AvaI	CYCGRG	1 cut(s)	2295
AvaII	GGWCC	3 cut(s)	98, 2305, 2390
BaeGI	GKGCMC	1 cut(s)	1405
BamHI	GGATCC	1 cut(s)	1603
BanI	GGYRCC	1 cut(s)	444
BanII	GRGCYC	2 cut(s)	319, 2426
BbrPI	CACGTG	1 cut(s)	995
Bbv12I	GWGCWC	3 cut(s)	319, 1405, 2426
BbvI	GCAGC	5 cut(s)	516, 670, 718, 1107, 1546
BccI	CCATC	7 cut(s)	739, 1016, 1178, 1352, 1390, 1487, 2270
BceAI	ACGGC	2 cut(s)	713, 2401
BciT130I	CCWGG	5 cut(s)	255, 482, 792, 1227, 1682
BclI	TGATCA	2 cut(s)	1417, 1966
BcoDI	GTCTC	5 cut(s)	138, 354, 827, 1719, 2042
BfaI	CTAG	3 cut(s)	1392, 1461, 1698
BfmI	CTRYAG	3 cut(s)	198, 1096, 1287
BfrI	CTTAAG	1 cut(s)	968
BglI	GCCNNNNNGGC	1 cut(s)	544
BisI	GCNGC	6 cut(s)	505, 593, 684, 707, 1121, 1560
BlsI	GCNGC	6 cut(s)	506, 594, 685, 708, 1122, 1561
BmcAI	AGTACT	1 cut(s)	2149
Bme1390I	CCNGG	5 cut(s)	255, 482, 792, 1227, 1682
Bme18I	GGWCC	3 cut(s)	98, 2305, 2390
BmeT110I	CYCGRG	1 cut(s)	2295
BmgT120I	GGNCC	5 cut(s)	98, 522, 2209, 2305, 2390
BmiI	GGNNCC	5 cut(s)	99, 446, 1411, 1605, 2306
BmrFI	CCNGG	5 cut(s)	255, 482, 792, 1227, 1682
BmrI	ACTGGG	1 cut(s)	891
BmsI	GCATC	7 cut(s)	245, 481, 573, 752, 1534, 1822, 2127
BmuI	ACTGGG	1 cut(s)	891
BoxI	GACNNNNGTC	1 cut(s)	2054
BpmI	CTGGAG	5 cut(s)	46, 165, 237, 303, 1554
Bpu10I	CCTNAGC	1 cut(s)	518
BpuEI	CTTGAG	2 cut(s)	974, 1737
BsaAI	YACGTR	1 cut(s)	995
BsaBI	GATNNNNATC	1 cut(s)	1965
BsaI	GGTCTC	2 cut(s)	354, 827
BsaJI	CCNNGG	5 cut(s)	173, 525, 642, 1226, 1881
BsaWI	WCCGGW	2 cut(s)	103, 441
Bsc4I	CCNNNNNNNGG	5 cut(s)	1008, 1009, 1232, 1657, 2206
Bse118I	RCCGGY	3 cut(s)	103, 127, 441
Bse1I	ACTGG	9 cut(s)	39, 320, 377, 886, 1514, 1537, 2177, 2200, 2272
Bse3DI	GCAATG	4 cut(s)	1524, 1708, 2006, 2241
Bse8I	GATNNNNATC	1 cut(s)	1965
BseBI	CCWGG	5 cut(s)	255, 482, 792, 1227, 1682
BseDI	CCNNGG	5 cut(s)	173, 525, 642, 1226, 1881
BseGI	GGATG	6 cut(s)	767, 1170, 1401, 1837, 1893, 2365
BseJI	GATNNNNATC	1 cut(s)	1965
BseLI	CCNNNNNNNGG	5 cut(s)	1008, 1009, 1232, 1657, 2206
BseMI	GCAATG	4 cut(s)	1524, 1708, 2006, 2241
BseMII	CTCAG	6 cut(s)	204, 273, 365, 649, 1214, 2365
BseNI	ACTGG	9 cut(s)	39, 320, 377, 886, 1514, 1537, 2177, 2200, 2272
BseRI	GAGGAG	3 cut(s)	1394, 2378, 2416
BseSI	GKGCMC	1 cut(s)	1405
BseXI	GCAGC	5 cut(s)	516, 670, 718, 1107, 1546
BseYI	CCCAGC	1 cut(s)	2211
BsgI	GTGCAG	1 cut(s)	1319
Bsh1236I	CGCG	2 cut(s)	778, 1569
BshFI	GGCC	6 cut(s)	524, 700, 749, 1154, 1221, 2210
BshNI	GGYRCC	1 cut(s)	444
BshTI	ACCGGT	2 cut(s)	103, 441
BsiHKAI	GWGCWC	3 cut(s)	319, 1405, 2426
BsiHKCI	CYCGRG	1 cut(s)	2295
BsiSI	CCGG	3 cut(s)	104, 128, 442
BslFI	GGGAC	3 cut(s)	30, 624, 2318
BslI	CCNNNNNNNGG	5 cut(s)	1008, 1009, 1232, 1657, 2206
BsmAI	GTCTC	5 cut(s)	138, 354, 827, 1719, 2042
BsmFI	GGGAC	3 cut(s)	30, 624, 2318
BsmI	GAATGC	1 cut(s)	1347
BsnI	GGCC	6 cut(s)	524, 700, 749, 1154, 1221, 2210
Bso31I	GGTCTC	2 cut(s)	354, 827
BsoBI	CYCGRG	1 cut(s)	2295
Bsp1286I	GDGCHC	3 cut(s)	319, 1405, 2426
Bsp1407I	TGTACA	1 cut(s)	1332
Bsp143I	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
Bsp19I	CCATGG	1 cut(s)	525
BspACI	CCGC	8 cut(s)	53, 62, 592, 776, 1838, 1844, 2001, 2388
BspANI	GGCC	6 cut(s)	524, 700, 749, 1154, 1221, 2210
BspCNI	CTCAG	6 cut(s)	203, 272, 364, 648, 1213, 2364
BspFNI	CGCG	2 cut(s)	778, 1569
BspLI	GGNNCC	5 cut(s)	99, 446, 1411, 1605, 2306
BspMAI	CTGCAG	1 cut(s)	1291
BspPI	GGATC	4 cut(s)	1312, 1598, 1611, 2011
BspT107I	GGYRCC	1 cut(s)	444
BspTI	CTTAAG	1 cut(s)	968
BspTNI	GGTCTC	2 cut(s)	354, 827
BsrBI	CCGCTC	1 cut(s)	2001
BsrDI	GCAATG	4 cut(s)	1524, 1708, 2006, 2241
BsrFI	RCCGGY	3 cut(s)	103, 127, 441
BsrGI	TGTACA	1 cut(s)	1332
BsrI	ACTGG	9 cut(s)	39, 320, 377, 886, 1514, 1537, 2177, 2200, 2272
BssAI	RCCGGY	3 cut(s)	103, 127, 441
BssECI	CCNNGG	5 cut(s)	173, 525, 642, 1226, 1881
BssMI	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
BssT1I	CCWWGG	4 cut(s)	173, 525, 642, 1881
Bst2UI	CCWGG	5 cut(s)	255, 482, 792, 1227, 1682
Bst4CI	ACNGT	6 cut(s)	340, 612, 624, 1991, 2317, 2411
Bst6I	CTCTTC	4 cut(s)	735, 1287, 1371, 1584
BstAFI	CTTAAG	1 cut(s)	968
BstAUI	TGTACA	1 cut(s)	1332
BstBAI	YACGTR	1 cut(s)	995
BstC8I	GCNNGC	7 cut(s)	315, 904, 1156, 1257, 2208, 2217, 2292
BstDSI	CCRYGG	1 cut(s)	525
BstENI	CCTNNNNNAGG	1 cut(s)	1655
BstF5I	GGATG	6 cut(s)	767, 1170, 1401, 1837, 1893, 2365
BstFNI	CGCG	2 cut(s)	778, 1569
BstKTI	GATC	8 cut(s)	714, 1320, 1420, 1606, 1795, 1969, 2006, 2113
BstMAI	GTCTC	5 cut(s)	138, 354, 827, 1719, 2042
BstMBI	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
BstMWI	GCNNNNNNNGC	5 cut(s)	61, 544, 706, 2212, 2216
BstNI	CCWGG	5 cut(s)	255, 482, 792, 1227, 1682
BstNSI	RCATGY	1 cut(s)	564
BstPAI	GACNNNNGTC	1 cut(s)	2054
BstSCI	CCNGG	5 cut(s)	253, 480, 790, 1225, 1680
BstSFI	CTRYAG	3 cut(s)	198, 1096, 1287
BstSLI	GKGCMC	1 cut(s)	1405
BstUI	CGCG	2 cut(s)	778, 1569
BstV1I	GCAGC	5 cut(s)	516, 670, 718, 1107, 1546
BstX2I	RGATCY	1 cut(s)	1603
BstYI	RGATCY	1 cut(s)	1603
BsuRI	GGCC	6 cut(s)	524, 700, 749, 1154, 1221, 2210
BtgI	CCRYGG	1 cut(s)	525
BtsCI	GGATG	6 cut(s)	767, 1170, 1401, 1837, 1893, 2365
BtsIMutI	CAGTG	4 cut(s)	327, 915, 2184, 2265
Cac8I	GCNNGC	7 cut(s)	315, 904, 1156, 1257, 2208, 2217, 2292
CaiI	CAGNNNCTG	3 cut(s)	197, 1232, 2206
Cfr10I	RCCGGY	3 cut(s)	103, 127, 441
Cfr13I	GGNCC	5 cut(s)	98, 522, 2209, 2305, 2390
CsiI	ACCWGGT	1 cut(s)	790
Csp6I	GTAC	6 cut(s)	620, 1333, 2096, 2148, 2335, 2412
CspAI	ACCGGT	2 cut(s)	103, 441
CviAII	CATG	5 cut(s)	526, 561, 1124, 1136, 1406
CviQI	GTAC	6 cut(s)	620, 1333, 2096, 2148, 2335, 2412
DpnI	GATC	8 cut(s)	713, 1319, 1419, 1605, 1794, 1968, 2005, 2112
DpnII	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
DraI	TTTAAA	2 cut(s)	873, 2074
EaeI	YGGCCR	2 cut(s)	698, 747
Eam1104I	CTCTTC	4 cut(s)	735, 1287, 1371, 1584
EarI	CTCTTC	4 cut(s)	735, 1287, 1371, 1584
EciI	GGCGGA	2 cut(s)	1827, 2403
Ecl136II	GAGCTC	2 cut(s)	317, 2424
Eco130I	CCWWGG	4 cut(s)	173, 525, 642, 1881
Eco24I	GRGCYC	2 cut(s)	319, 2426
Eco31I	GGTCTC	2 cut(s)	354, 827
Eco47I	GGWCC	3 cut(s)	98, 2305, 2390
Eco53kI	GAGCTC	2 cut(s)	317, 2424
Eco57I	CTGAAG	6 cut(s)	216, 228, 561, 918, 939, 1701
Eco72I	CACGTG	1 cut(s)	995
Eco88I	CYCGRG	1 cut(s)	2295
EcoICRI	GAGCTC	2 cut(s)	317, 2424
EcoNI	CCTNNNNNAGG	1 cut(s)	1655
EcoRI	GAATTC	1 cut(s)	1778
EcoRII	CCWGG	5 cut(s)	253, 480, 790, 1225, 1680
EcoT14I	CCWWGG	4 cut(s)	173, 525, 642, 1881
EcoT22I	ATGCAT	2 cut(s)	566, 1549
EcoT38I	GRGCYC	2 cut(s)	319, 2426
ErhI	CCWWGG	4 cut(s)	173, 525, 642, 1881
FaeI	CATG	5 cut(s)	529, 564, 1127, 1139, 1409
FalI	AAGNNNNNCTT	2 cut(s)	1626, 1658
FaqI	GGGAC	3 cut(s)	30, 624, 2318
FatI	CATG	5 cut(s)	525, 560, 1123, 1135, 1405
FauI	CCCGC	1 cut(s)	60
FauNDI	CATATG	1 cut(s)	2434
FbaI	TGATCA	2 cut(s)	1417, 1966
Fnu4HI	GCNGC	6 cut(s)	505, 593, 684, 707, 1121, 1560
FokI	GGATG	6 cut(s)	774, 1157, 1408, 1844, 1900, 2372
FriOI	GRGCYC	2 cut(s)	319, 2426
Fsp4HI	GCNGC	6 cut(s)	505, 593, 684, 707, 1121, 1560
FspBI	CTAG	3 cut(s)	1392, 1461, 1698
GluI	GCNGC	6 cut(s)	505, 593, 684, 707, 1121, 1560
GsaI	CCCAGC	1 cut(s)	2215
GsuI	CTGGAG	5 cut(s)	46, 165, 237, 303, 1554
HaeIII	GGCC	6 cut(s)	524, 700, 749, 1154, 1221, 2210
HapII	CCGG	3 cut(s)	104, 128, 442
Hin1II	CATG	5 cut(s)	529, 564, 1127, 1139, 1409
HincII	GTYRAC	1 cut(s)	1130
HindII	GTYRAC	1 cut(s)	1130
HindIII	AAGCTT	2 cut(s)	1205, 2217
HinfI	GANTC	9 cut(s)	71, 147, 194, 473, 584, 815, 855, 2328, 2399
HpaII	CCGG	3 cut(s)	104, 128, 442
HphI	GGTGA	3 cut(s)	49, 231, 2180
Hpy166II	GTNNAC	6 cut(s)	101, 608, 992, 1130, 1284, 1403
Hpy188III	TCNNGA	8 cut(s)	183, 627, 938, 1233, 1517, 1616, 1787, 1829
Hpy8I	GTNNAC	6 cut(s)	101, 608, 992, 1130, 1284, 1403
HpyCH4III	ACNGT	6 cut(s)	340, 612, 624, 1991, 2317, 2411
HpyCH4IV	ACGT	1 cut(s)	994
HpyF10VI	GCNNNNNNNGC	5 cut(s)	61, 544, 706, 2212, 2216
HpySE526I	ACGT	1 cut(s)	994
Hsp92II	CATG	5 cut(s)	529, 564, 1127, 1139, 1409
Ksp22I	TGATCA	2 cut(s)	1417, 1966
Kzo9I	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
Lsp1109I	GCAGC	5 cut(s)	516, 670, 718, 1107, 1546
LweI	GCATC	7 cut(s)	245, 481, 573, 752, 1534, 1822, 2127
MabI	ACCWGGT	1 cut(s)	790
MaeI	CTAG	3 cut(s)	1392, 1461, 1698
MaeII	ACGT	1 cut(s)	994
MaeIII	GTNAC	8 cut(s)	148, 295, 325, 437, 556, 995, 1747, 2311
MalI	GATC	8 cut(s)	713, 1319, 1419, 1605, 1794, 1968, 2005, 2112
MbiI	CCGCTC	1 cut(s)	2001
MboI	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
MfeI	CAATTG	2 cut(s)	508, 576
MflI	RGATCY	1 cut(s)	1603
MhlI	GDGCHC	3 cut(s)	319, 1405, 2426
MlyI	GAGTC	2 cut(s)	156, 482
Mph1103I	ATGCAT	2 cut(s)	566, 1549
MroXI	GAANNNNTTC	2 cut(s)	117, 1499
MseI	TTAA	7 cut(s)	731, 872, 969, 1676, 1875, 2073, 2144
MspA1I	CMGCKG	1 cut(s)	709
MspCI	CTTAAG	1 cut(s)	968
MspI	CCGG	3 cut(s)	104, 128, 442
MspR9I	CCNGG	5 cut(s)	255, 482, 792, 1227, 1682
MunI	CAATTG	2 cut(s)	508, 576
Mva1269I	GAATGC	1 cut(s)	1347
MvaI	CCWGG	5 cut(s)	255, 482, 792, 1227, 1682
MvnI	CGCG	2 cut(s)	778, 1569
MwoI	GCNNNNNNNGC	5 cut(s)	61, 544, 706, 2212, 2216
NcoI	CCATGG	1 cut(s)	525
NdeI	CATATG	1 cut(s)	2434
NdeII	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
NlaIII	CATG	5 cut(s)	529, 564, 1127, 1139, 1409
NlaIV	GGNNCC	5 cut(s)	99, 446, 1411, 1605, 2306
NmuCI	GTSAC	6 cut(s)	148, 325, 556, 995, 1747, 2311
NsiI	ATGCAT	2 cut(s)	566, 1549
NspI	RCATGY	1 cut(s)	564
PctI	GAATGC	1 cut(s)	1347
PdmI	GAANNNNTTC	2 cut(s)	117, 1499
PfeI	GAWTC	7 cut(s)	71, 194, 584, 815, 855, 2328, 2399
PflFI	GACNNNGTC	1 cut(s)	2126
PflMI	CCANNNNNTGG	2 cut(s)	1232, 2206
PinAI	ACCGGT	2 cut(s)	103, 441
PkrI	GCNGC	6 cut(s)	506, 594, 685, 708, 1122, 1561
PleI	GAGTC	2 cut(s)	155, 481
PmaCI	CACGTG	1 cut(s)	995
PmlI	CACGTG	1 cut(s)	995
PpsI	GAGTC	2 cut(s)	155, 481
Ppu21I	YACGTR	1 cut(s)	995
PshAI	GACNNNNGTC	1 cut(s)	2054
Psp124BI	GAGCTC	2 cut(s)	319, 2426
Psp6I	CCWGG	5 cut(s)	253, 480, 790, 1225, 1680
PspCI	CACGTG	1 cut(s)	995
PspFI	CCCAGC	1 cut(s)	2211
PspGI	CCWGG	5 cut(s)	253, 480, 790, 1225, 1680
PspN4I	GGNNCC	5 cut(s)	99, 446, 1411, 1605, 2306
PspPI	GGNCC	5 cut(s)	98, 522, 2209, 2305, 2390
PsrI	GAACNNNNNNTAC	2 cut(s)	708, 740
PstI	CTGCAG	1 cut(s)	1291
PstNI	CAGNNNCTG	3 cut(s)	197, 1232, 2206
PsuI	RGATCY	1 cut(s)	1603
PsyI	GACNNNGTC	1 cut(s)	2126
PvuII	CAGCTG	1 cut(s)	709
RsaI	GTAC	6 cut(s)	621, 1334, 2097, 2149, 2336, 2413
RsaNI	GTAC	6 cut(s)	620, 1333, 2096, 2148, 2335, 2412
SacI	GAGCTC	2 cut(s)	319, 2426
SaqAI	TTAA	7 cut(s)	731, 872, 969, 1676, 1875, 2073, 2144
SatI	GCNGC	6 cut(s)	505, 593, 684, 707, 1121, 1560
Sau3AI	GATC	8 cut(s)	711, 1317, 1417, 1603, 1792, 1966, 2003, 2110
Sau96I	GGNCC	5 cut(s)	98, 522, 2209, 2305, 2390
ScaI	AGTACT	1 cut(s)	2149
SchI	GAGTC	2 cut(s)	156, 482
ScrFI	CCNGG	5 cut(s)	255, 482, 792, 1227, 1682
SduI	GDGCHC	3 cut(s)	319, 1405, 2426
SexAI	ACCWGGT	1 cut(s)	790
SfaNI	GCATC	7 cut(s)	245, 481, 573, 752, 1534, 1822, 2127
SfcI	CTRYAG	3 cut(s)	198, 1096, 1287
SinI	GGWCC	3 cut(s)	98, 2305, 2390
SmiI	ATTTAAAT	1 cut(s)	873
SmlI	CTYRAG	3 cut(s)	953, 968, 1752
SmoI	CTYRAG	3 cut(s)	953, 968, 1752
SsiI	CCGC	8 cut(s)	53, 62, 592, 776, 1838, 1844, 2001, 2388
SspMI	CTAG	3 cut(s)	1392, 1461, 1698
SstI	GAGCTC	2 cut(s)	319, 2426
StyD4I	CCNGG	5 cut(s)	253, 480, 790, 1225, 1680
StyI	CCWWGG	4 cut(s)	173, 525, 642, 1881
SwaI	ATTTAAAT	1 cut(s)	873
TaaI	ACNGT	6 cut(s)	340, 612, 624, 1991, 2317, 2411
TaiI	ACGT	1 cut(s)	997
TaqI	TCGA	5 cut(s)	614, 937, 1795, 2272, 2319
TaqII	GACCGA	2 cut(s)	196, 2322
TatI	WGTACW	4 cut(s)	1332, 2147, 2334, 2411
TauI	GCSGC	1 cut(s)	595
TfiI	GAWTC	7 cut(s)	71, 194, 584, 815, 855, 2328, 2399
Tru1I	TTAA	7 cut(s)	731, 872, 969, 1676, 1875, 2073, 2144
Tru9I	TTAA	7 cut(s)	731, 872, 969, 1676, 1875, 2073, 2144
TscAI	CASTG	4 cut(s)	327, 922, 2184, 2272
TseFI	GTSAC	6 cut(s)	148, 325, 556, 995, 1747, 2311
TseI	GCWGC	5 cut(s)	504, 683, 706, 1120, 1559
Tsp45I	GTSAC	6 cut(s)	148, 325, 556, 995, 1747, 2311
TspGWI	ACGGA	2 cut(s)	717, 924
TspRI	CASTG	4 cut(s)	327, 922, 2184, 2272
Tth111I	GACNNNGTC	1 cut(s)	2126
Van91I	CCANNNNNTGG	2 cut(s)	1232, 2206
Vha464I	CTTAAG	1 cut(s)	968
VneI	GTGCAC	1 cut(s)	1401
VpaK11BI	GGWCC	3 cut(s)	98, 2305, 2390
XagI	CCTNNNNNAGG	1 cut(s)	1655
XapI	RAATTY	3 cut(s)	868, 874, 1778
XceI	RCATGY	1 cut(s)	564
XmnI	GAANNNNTTC	2 cut(s)	117, 1499
XspI	CTAG	3 cut(s)	1392, 1461, 1698
ZrmI	AGTACT	1 cut(s)	2149
Zsp2I	ATGCAT	2 cut(s)	566, 1549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.