Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002175

Physical Location & Seq

Genomic Coordinates Reverse (-)

3028530 .. 3036057

Gene Structure 7528 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1025000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGAGCAAAAGGAAGGAGAAAACTGGAGGGAACTGGTGAGAAAGATGCTTCCACCAGGTGCTTCAATCCCTGAAGATGCTGCTGAACTTGATTACTCTATAGCTATGGAGTATGAGGGCCCTCCTGTCTCTTATGACGTACCTAGGGTCGAACCCCTTGATGTTAATCCACATGGAATCCCAACTGCAGAAACGCTATCTGAATCACAAAGATCGGTTACCAGTACCGGTCCACCAGTTATTGAACCAATTCCTCTGCCTGTGTCTCGTATTGCTGGTGTCACAAGTTCCCCTACCCAAAGTCCAAGGGTATCAGGAAGCTCAGAGTCCGTAGTGTCTGTCTTGCAAAACCATGATTTTTCTTCAGCCTCGCCTTCAGCCTCTCCAGGTTCAGTTCATAATCCTCCAAGTACCCGTCCCAAACAGGTTGTCAATGAAGTAAAGCGAGCTCCCGTTGTCACTTTTAATACTGTAGATAGGTCAGAGAGGAAAGAGGTTGATGCTGAGAAGCCAGCTTTTCCAGCTTATGTTGCTGTTTCTAAGGAGAAGAAGAAAAAAAAGAGTAGAGCTTGTTATCGTTGCCAAAAAGGAAAATGGGAAACTAAGGAATCGTGCCTGGTTTGTGATGCTAAGTACTGCAGCAATTGTGTGCTTAGGGCAATGGGTTCCATGCCCGAAGGGCGTAAGTGTGTGACATGCATTGGTCAGCCAATTGATGAATCAAAGCGGTTAAAACTGGGTAAACAGTCAAGGGTATTGTCCAGATTGCTTAGCCCATTGGAAGTCAAGCAGATAATGAAAGCAGAAAAAGAATGTTCTGCTAATCAGCTTCGGCCGGAGCAGCTTATAGTGAATGGATTCCCTCTAAAGCCAGAAGAGATGGCTGAATTACTTGGATGTCCTTTACCTCCAAGGAAGTTGAAGCCCGGTAGATATTGGTATGATAAAGAATCTGGTCTCTGGGGAAAGGAGGGAGAAAGACCTGATAGACTCATAAGTTCAAACCTAAATTTTAACGGGAAACTTAGCTCCGATGCAAGCAATGGAAACACTGAGGTCTACCTCAATGGCCGAGAAATTACAAAGCTTGAACTCAGGGTCCTAAAGGTAGCAAATGTGCAATGCCCACGTGACACCCATTTTTGGGTCTATGATGACGGACGCTATGAGGAAGAAGGTCAGAATAATATCAGAGGAAATATTTGGGAGAAGGCAACAACTCGGTTTCTCTGTTCCCTATTCTCTTTGCCTGTGCCTCACGGTCAACCTAATGGACCAAGAGATGAGCCTAGCAACTATACCACTGTTCCAAATTATTTGGAGCAGAAAAAAGTTCAGAAGCTGCTTCTCCTTGGACTTCAAGGGTCTGGGACCAGCACAATCTTCAAACAGGCCAAGTTCTTGTATGGAAACAAGTTTACTTCAGAAGAACTGCAGGATATAAAGCTGATGATCCAGAGTAACATGTACAAATATCTAAGCATTCTGCTTGATGGACGAGAACGCTTTGAAGAAGAGGCTTTGTCTAGGATGGAAGGACATGGTTCTCACGATCAAAACGCTGAAGCAGGTGGTGAGGTCAACCAAGATGAAACTACTCAATGTGTCTACTCGATTAACCCGAGGTTGAAGCATTTCTCTGACTGGCTTCTAGACATTATTGCTACTGGAGATCTAGATGCATTCTTCCCTGCTGCAACACGCGAGTATGCTCCATTAGTTGAAGAAGTGTGGAAGGATCCTGCTATTCAGGAAACATATAAAAGAAGAGATGAGCTTCATTTCCTCCCTAATGTTGCAGAGTACTTTTTAAGCCAGGCTGTTGAGGTATCTAGCAATGAGTATGAACCTTCAGAACGAGATATACTATATGCAGAAGGGGTCACTCAAGGAAATGGATTGGCTTTCATAGAATTCACTCTTGATGAACGGAGTCCAATGTCTGAAACCTACACGGAAAATACTGAAGCTCCACCCCCGCCTCTAACCAAATACCAACTTATAAGAGTGAATGCGAAGGGAATGAATGAAGGGTGCAAGTGGGTGGAAATGTTTGAAGATGTACGTGTGGTAGTGTTTTGCGTCGCTCTGAGTGATTATGATCAGATGTGGGTTTCCCCAGACAATAGTGGCAGTGGAGCGTTACTTCAAAATAAGATGATACAGAGCAAGGAGCTCTTTGAGAATATGGTCAGGCACCCTTGCTTCAAGGATACCCCTTTCGTATTAATACTAAACAAGTACGATCTCTTTGAGGACAAGGTCAATCAGGCGCCATTAAGTTGTTGCGAATGGTTCAATGACTTTAGTCCGGTGAAGCCCCACCACAATAATCAGTCACTGGCCCAGCAAGCTTACTTCTACGTTGCAATGAAGTTCAAAGATCTTTATGCTTCCATCACAGGCCGAAAGCTTTTTGTGTGGCAGACTCGGGCAAGGGACCGAGTGACTGTCGATGAGGCATTCAAGTACATAAGGGAGGTTCTGAAGTGGGATGACGAGAAGGAAGAGAACTACTATGGCGGACCAGAGGATTCGTTTTACAGTACAGACATGAGCTCCTCACCTTATGGAAGGCAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009723 GO:0009725 GO:0009898 GO:0009987 GO:0010033 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

862

Amino Acids

97.62

Weight (kDa)

5.43

Isoelectric Point (pI)

47.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	441 - 828	1.7e-75	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930
fragaria_vesca	FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320
malus_domestica	MD06G1025000.v1.1
prunus_persica	Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1
pyrus_communis	pycom06g02030
rosa_chinensis	RchiOBHm_Chr7g0212271
rosa_laevigata	RLG00000002900
rosa_multiflora	Rmu_sc0001911.1_g000047
rosa_roxburghii	Rroxscaffold_3G00246940
rosa_rugosa	Rorug07G0133000 Rorug07G0133100 Rorug07G0133200 Rorug07G0133200
rosa_samantha	Rh7AG265800 Rh7BG260000
rosa_wichuraiana	Rw7G022760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2003
AarI	CACCTGC	1 cut(s)	1559
Acc36I	ACCTGC	1 cut(s)	1559
AccB1I	GGYRCC	2 cut(s)	2196, 2272
AccI	GTMKAC	2 cut(s)	1059, 1608
AccII	CGCG	1 cut(s)	1704
AciI	CCGC	3 cut(s)	727, 1979, 2523
AclWI	GGATC	3 cut(s)	1447, 1733, 1746
AcoI	YGGCCR	2 cut(s)	833, 1069
AcsI	RAATTY	2 cut(s)	1009, 1913
AcuI	CTGAAG	8 cut(s)	93, 348, 360, 1407, 1584, 1836, 1986, 2507
AcvI	CACGTG	1 cut(s)	1130
AcyI	GRCGYC	1 cut(s)	2273
AdeI	CACNNNGTG	1 cut(s)	59
AfiI	CCNNNNNNNGG	2 cut(s)	1143, 1144
AflIII	ACRYGT	2 cut(s)	1464, 2065
AgeI	ACCGGT	1 cut(s)	227
AjnI	CCWGG	4 cut(s)	55, 385, 615, 1815
AloI	GAACNNNNNNTCC	2 cut(s)	1083, 1115
Alw21I	GWGCWC	3 cut(s)	451, 2178, 2561
Alw26I	GTCTC	3 cut(s)	133, 270, 962
AlwI	GGATC	3 cut(s)	1447, 1733, 1746
AlwNI	CAGNNNCTG	2 cut(s)	1342, 2341
Ama87I	CYCGRG	2 cut(s)	1621, 2430
AoxI	GGCC	6 cut(s)	118, 833, 1069, 1392, 2343, 2404
ApaI	GGGCCC	1 cut(s)	122
ApeKI	GCWGC	5 cut(s)	80, 639, 841, 1342, 1694
ApoI	RAATTY	2 cut(s)	1009, 1913
ArsI	GACNNNNNNTTYG	2 cut(s)	414, 446
AseI	ATTAAT	1 cut(s)	2228
AsiGI	ACCGGT	1 cut(s)	227
Asp700I	GAANNNNTTC	3 cut(s)	249, 857, 1634
AspA2I	CCTAGG	1 cut(s)	143
AspLEI	GCGC	1 cut(s)	2275
AspS9I	GGNCC	9 cut(s)	118, 119, 230, 1099, 1274, 1371, 2344, 2440, 2525
AsuC2I	CCSGG	1 cut(s)	927
AsuHPI	GGTGA	4 cut(s)	49, 1586, 2326, 2556
AvaI	CYCGRG	2 cut(s)	1621, 2430
AvaII	GGWCC	6 cut(s)	230, 1099, 1274, 1371, 2440, 2525
AvrII	CCTAGG	1 cut(s)	143
BaeGI	GKGCMC	1 cut(s)	122
BamHI	GGATCC	1 cut(s)	1738
BanI	GGYRCC	2 cut(s)	2196, 2272
BanII	GRGCYC	4 cut(s)	122, 451, 2178, 2561
BbrPI	CACGTG	1 cut(s)	1130
Bbv12I	GWGCWC	3 cut(s)	451, 2178, 2561
BbvI	GCAGC	5 cut(s)	67, 651, 853, 1329, 1681
BccI	CCATC	4 cut(s)	874, 1487, 1525, 2405
BciT130I	CCWGG	4 cut(s)	57, 387, 617, 1817
BciVI	GTATCC	1 cut(s)	2206
BclI	TGATCA	1 cut(s)	2101
BcnI	CCSGG	1 cut(s)	927
BcoDI	GTCTC	3 cut(s)	133, 270, 962
BfaI	CTAG	6 cut(s)	144, 1290, 1527, 1652, 1676, 1833
BfmI	CTRYAG	5 cut(s)	99, 186, 471, 637, 1433
BfoI	RGCGCY	1 cut(s)	2276
BfuAI	ACCTGC	1 cut(s)	1559
BfuI	GTATCC	1 cut(s)	2206
BglI	GCCNNNNNGGC	1 cut(s)	679
BglII	AGATCT	2 cut(s)	1672, 2383
BisI	GCNGC	5 cut(s)	81, 640, 842, 1343, 1695
BlnI	CCTAGG	1 cut(s)	143
BlpI	GCTNAGC	1 cut(s)	770
BlsI	GCNGC	5 cut(s)	82, 641, 843, 1344, 1696
BmcAI	AGTACT	2 cut(s)	635, 1805
Bme1390I	CCNGG	5 cut(s)	57, 387, 617, 927, 1817
Bme18I	GGWCC	6 cut(s)	230, 1099, 1274, 1371, 2440, 2525
BmeT110I	CYCGRG	2 cut(s)	1621, 2430
BmgT120I	GGNCC	9 cut(s)	118, 119, 230, 1099, 1274, 1371, 2344, 2440, 2525
BmiI	GGNNCC	8 cut(s)	120, 667, 1100, 1372, 1740, 2198, 2274, 2441
BmrFI	CCNGG	5 cut(s)	57, 387, 617, 927, 1817
BmrI	ACTGGG	1 cut(s)	746
BmsI	GCATC	6 cut(s)	36, 67, 490, 616, 1024, 1669
BmuI	ACTGGG	1 cut(s)	746
BoxI	GACNNNNGTC	1 cut(s)	2307
BpmI	CTGGAG	3 cut(s)	46, 369, 1689
Bpu10I	CCTNAGC	1 cut(s)	653
Bpu1102I	GCTNAGC	1 cut(s)	770
BpuEI	CTTGAG	1 cut(s)	1872
BpuMI	CCSGG	1 cut(s)	927
BsaAI	YACGTR	2 cut(s)	1130, 2066
BsaBI	GATNNNNATC	2 cut(s)	165, 2100
BsaHI	GRCGYC	1 cut(s)	2273
BsaI	GGTCTC	1 cut(s)	962
BsaJI	CCNNGG	5 cut(s)	143, 305, 911, 1351, 1622
BsaWI	WCCGGW	2 cut(s)	227, 2311
BsaXI	ACNNNNNCTCC	4 cut(s)	1954, 1984, 2543, 2573
Bsc4I	CCNNNNNNNGG	2 cut(s)	1143, 1144
Bse118I	RCCGGY	1 cut(s)	227
Bse1I	ACTGG	8 cut(s)	29, 39, 222, 236, 741, 1649, 1672, 2346
Bse3DI	GCAATG	5 cut(s)	666, 1048, 1127, 1843, 2376
Bse8I	GATNNNNATC	2 cut(s)	165, 2100
BseBI	CCWGG	4 cut(s)	57, 387, 617, 1817
BseDI	CCNNGG	5 cut(s)	143, 305, 911, 1351, 1622
BseGI	GGATG	3 cut(s)	902, 1536, 2500
BseJI	GATNNNNATC	2 cut(s)	165, 2100
BseLI	CCNNNNNNNGG	2 cut(s)	1143, 1144
BseMI	GCAATG	5 cut(s)	666, 1048, 1127, 1843, 2376
BseMII	CTCAG	5 cut(s)	336, 495, 1044, 1108, 2081
BseNI	ACTGG	8 cut(s)	29, 39, 222, 236, 741, 1649, 1672, 2346
BseRI	GAGGAG	1 cut(s)	2551
BseSI	GKGCMC	1 cut(s)	122
BseX3I	CGGCCG	1 cut(s)	833
BseXI	GCAGC	5 cut(s)	67, 651, 853, 1329, 1681
BseYI	CCCAGC	1 cut(s)	2346
Bsh1236I	CGCG	1 cut(s)	1704
Bsh1285I	CGRYCG	1 cut(s)	836
BshFI	GGCC	6 cut(s)	120, 835, 1071, 1394, 2345, 2406
BshNI	GGYRCC	2 cut(s)	2196, 2272
BshTI	ACCGGT	1 cut(s)	227
BsiEI	CGRYCG	1 cut(s)	836
BsiHKAI	GWGCWC	3 cut(s)	451, 2178, 2561
BsiHKCI	CYCGRG	2 cut(s)	1621, 2430
BsiSI	CCGG	4 cut(s)	228, 836, 927, 2312
BslFI	GGGAC	3 cut(s)	402, 1384, 2453
BslI	CCNNNNNNNGG	2 cut(s)	1143, 1144
BsmAI	GTCTC	3 cut(s)	133, 270, 962
BsmFI	GGGAC	3 cut(s)	402, 1384, 2453
BsmI	GAATGC	4 cut(s)	1482, 1682, 2017, 2462
BsnI	GGCC	6 cut(s)	120, 835, 1071, 1394, 2345, 2406
Bso31I	GGTCTC	1 cut(s)	962
BsoBI	CYCGRG	2 cut(s)	1621, 2430
Bsp120I	GGGCCC	1 cut(s)	118
Bsp1286I	GDGCHC	4 cut(s)	122, 451, 2178, 2561
Bsp1407I	TGTACA	1 cut(s)	1467
Bsp143I	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
Bsp1720I	GCTNAGC	1 cut(s)	770
BspACI	CCGC	3 cut(s)	727, 1979, 2523
BspANI	GGCC	6 cut(s)	120, 835, 1071, 1394, 2345, 2406
BspCNI	CTCAG	5 cut(s)	335, 496, 1045, 1107, 2082
BspFNI	CGCG	1 cut(s)	1704
BspLI	GGNNCC	8 cut(s)	120, 667, 1100, 1372, 1740, 2198, 2274, 2441
BspMAI	CTGCAG	3 cut(s)	190, 641, 1437
BspMI	ACCTGC	1 cut(s)	1559
BspPI	GGATC	3 cut(s)	1447, 1733, 1746
BspT107I	GGYRCC	2 cut(s)	2196, 2272
BspTNI	GGTCTC	1 cut(s)	962
BsrDI	GCAATG	5 cut(s)	666, 1048, 1127, 1843, 2376
BsrFI	RCCGGY	1 cut(s)	227
BsrGI	TGTACA	1 cut(s)	1467
BsrI	ACTGG	8 cut(s)	29, 39, 222, 236, 741, 1649, 1672, 2346
BssAI	RCCGGY	1 cut(s)	227
BssECI	CCNNGG	5 cut(s)	143, 305, 911, 1351, 1622
BssMI	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
BssNI	GRCGYC	1 cut(s)	2273
BssT1I	CCWWGG	4 cut(s)	143, 305, 911, 1351
Bst2UI	CCWGG	4 cut(s)	57, 387, 617, 1817
Bst4CI	ACNGT	6 cut(s)	472, 747, 1262, 1306, 2452, 2546
Bst6I	CTCTTC	4 cut(s)	870, 1509, 1762, 2502
BstACI	GRCGYC	1 cut(s)	2273
BstAUI	TGTACA	1 cut(s)	1467
BstBAI	YACGTR	2 cut(s)	1130, 2066
BstC8I	GCNNGC	4 cut(s)	447, 513, 1039, 2352
BstEII	GGTNACC	1 cut(s)	217
BstF5I	GGATG	3 cut(s)	902, 1536, 2500
BstFNI	CGCG	1 cut(s)	1704
BstH2I	RGCGCY	1 cut(s)	2276
BstHHI	GCGC	1 cut(s)	2275
BstKTI	GATC	8 cut(s)	215, 1455, 1555, 1675, 1741, 2104, 2248, 2386
BstMAI	GTCTC	3 cut(s)	133, 270, 962
BstMBI	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
BstMCI	CGRYCG	1 cut(s)	836
BstMWI	GCNNNNNNNGC	5 cut(s)	521, 530, 679, 841, 2351
BstNI	CCWGG	4 cut(s)	57, 387, 617, 1817
BstNSI	RCATGY	2 cut(s)	699, 1468
BstPAI	GACNNNNGTC	1 cut(s)	2307
BstPI	GGTNACC	1 cut(s)	217
BstSCI	CCNGG	5 cut(s)	55, 385, 615, 925, 1815
BstSFI	CTRYAG	5 cut(s)	99, 186, 471, 637, 1433
BstSLI	GKGCMC	1 cut(s)	122
BstUI	CGCG	1 cut(s)	1704
BstV1I	GCAGC	5 cut(s)	67, 651, 853, 1329, 1681
BstX2I	RGATCY	3 cut(s)	1672, 1738, 2383
BstYI	RGATCY	3 cut(s)	1672, 1738, 2383
BstZI	CGGCCG	1 cut(s)	833
BsuI	GTATCC	1 cut(s)	2206
BsuRI	GGCC	6 cut(s)	120, 835, 1071, 1394, 2345, 2406
BtsCI	GGATG	3 cut(s)	902, 1536, 2500
BtsI	GCAGTG	1 cut(s)	2140
BtsIMutI	CAGTG	4 cut(s)	1050, 1302, 2140, 2339
BveI	ACCTGC	1 cut(s)	1559
Cac8I	GCNNGC	4 cut(s)	447, 513, 1039, 2352
CaiI	CAGNNNCTG	2 cut(s)	1342, 2341
CfoI	GCGC	1 cut(s)	2275
Cfr10I	RCCGGY	1 cut(s)	227
Cfr13I	GGNCC	9 cut(s)	118, 119, 230, 1099, 1274, 1371, 2344, 2440, 2525
CseI	GACGC	2 cut(s)	1170, 2071
CsiI	ACCWGGT	1 cut(s)	55
CspAI	ACCGGT	1 cut(s)	227
CviAII	CATG	7 cut(s)	173, 353, 670, 696, 1465, 1541, 2554
DinI	GGCGCC	1 cut(s)	2274
DpnI	GATC	8 cut(s)	214, 1454, 1554, 1674, 1740, 2103, 2247, 2385
DpnII	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
DraIII	CACNNNGTG	1 cut(s)	59
EaeI	YGGCCR	2 cut(s)	833, 1069
EagI	CGGCCG	1 cut(s)	833
Eam1104I	CTCTTC	4 cut(s)	870, 1509, 1762, 2502
EarI	CTCTTC	4 cut(s)	870, 1509, 1762, 2502
EciI	GGCGGA	1 cut(s)	2538
Ecl136II	GAGCTC	3 cut(s)	449, 2176, 2559
EclXI	CGGCCG	1 cut(s)	833
Eco130I	CCWWGG	4 cut(s)	143, 305, 911, 1351
Eco24I	GRGCYC	4 cut(s)	122, 451, 2178, 2561
Eco31I	GGTCTC	1 cut(s)	962
Eco47I	GGWCC	6 cut(s)	230, 1099, 1274, 1371, 2440, 2525
Eco52I	CGGCCG	1 cut(s)	833
Eco53kI	GAGCTC	3 cut(s)	449, 2176, 2559
Eco57I	CTGAAG	8 cut(s)	93, 348, 360, 1407, 1584, 1836, 1986, 2507
Eco72I	CACGTG	1 cut(s)	1130
Eco88I	CYCGRG	2 cut(s)	1621, 2430
Eco91I	GGTNACC	1 cut(s)	217
EcoICRI	GAGCTC	3 cut(s)	449, 2176, 2559
EcoO109I	RGGNCCY	3 cut(s)	118, 119, 1099
EcoO65I	GGTNACC	1 cut(s)	217
EcoRI	GAATTC	1 cut(s)	1913
EcoRII	CCWGG	4 cut(s)	55, 385, 615, 1815
EcoT14I	CCWWGG	4 cut(s)	143, 305, 911, 1351
EcoT22I	ATGCAT	2 cut(s)	701, 1684
EcoT38I	GRGCYC	4 cut(s)	122, 451, 2178, 2561
EgeI	GGCGCC	1 cut(s)	2274
EheI	GGCGCC	1 cut(s)	2274
ErhI	CCWWGG	4 cut(s)	143, 305, 911, 1351
FaeI	CATG	7 cut(s)	176, 356, 673, 699, 1468, 1544, 2557
FalI	AAGNNNNNCTT	2 cut(s)	2162, 2194
FaqI	GGGAC	3 cut(s)	402, 1384, 2453
FatI	CATG	7 cut(s)	172, 352, 669, 695, 1464, 1540, 2553
FauI	CCCGC	1 cut(s)	1986
FbaI	TGATCA	1 cut(s)	2101
FblI	GTMKAC	2 cut(s)	1059, 1608
Fnu4HI	GCNGC	5 cut(s)	81, 640, 842, 1343, 1695
FokI	GGATG	3 cut(s)	909, 1543, 2507
FriOI	GRGCYC	4 cut(s)	122, 451, 2178, 2561
Fsp4HI	GCNGC	5 cut(s)	81, 640, 842, 1343, 1695
FspBI	CTAG	6 cut(s)	144, 1290, 1527, 1652, 1676, 1833
GlaI	GCGC	1 cut(s)	2274
GluI	GCNGC	5 cut(s)	81, 640, 842, 1343, 1695
GsaI	CCCAGC	1 cut(s)	2350
GsuI	CTGGAG	3 cut(s)	46, 369, 1689
HaeII	RGCGCY	1 cut(s)	2276
HaeIII	GGCC	6 cut(s)	120, 835, 1071, 1394, 2345, 2406
HapII	CCGG	4 cut(s)	228, 836, 927, 2312
HgaI	GACGC	2 cut(s)	1170, 2071
HhaI	GCGC	1 cut(s)	2275
Hin1I	GRCGYC	1 cut(s)	2273
Hin1II	CATG	7 cut(s)	176, 356, 673, 699, 1468, 1544, 2557
Hin6I	GCGC	1 cut(s)	2273
HinP1I	GCGC	1 cut(s)	2273
HincII	GTYRAC	2 cut(s)	1265, 1582
HindII	GTYRAC	2 cut(s)	1265, 1582
HindIII	AAGCTT	3 cut(s)	1085, 2352, 2411
HpaII	CCGG	4 cut(s)	228, 836, 927, 2312
HphI	GGTGA	4 cut(s)	49, 1586, 2326, 2556
Hpy166II	GTNNAC	7 cut(s)	233, 743, 1060, 1265, 1419, 1582, 1609
Hpy188III	TCNNGA	8 cut(s)	315, 762, 1456, 1550, 1652, 1676, 1751, 1922
Hpy8I	GTNNAC	7 cut(s)	233, 743, 1060, 1265, 1419, 1582, 1609
Hpy99I	CGWCG	1 cut(s)	2087
HpyCH4III	ACNGT	6 cut(s)	472, 747, 1262, 1306, 2452, 2546
HpyCH4IV	ACGT	4 cut(s)	138, 1129, 2065, 2364
HpyF10VI	GCNNNNNNNGC	5 cut(s)	521, 530, 679, 841, 2351
HpySE526I	ACGT	4 cut(s)	138, 1129, 2065, 2364
Hsp92I	GRCGYC	1 cut(s)	2273
Hsp92II	CATG	7 cut(s)	176, 356, 673, 699, 1468, 1544, 2557
HspAI	GCGC	1 cut(s)	2273
KasI	GGCGCC	1 cut(s)	2272
Ksp22I	TGATCA	1 cut(s)	2101
Kzo9I	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
Lsp1109I	GCAGC	5 cut(s)	67, 651, 853, 1329, 1681
LweI	GCATC	6 cut(s)	36, 67, 490, 616, 1024, 1669
MabI	ACCWGGT	1 cut(s)	55
MaeI	CTAG	6 cut(s)	144, 1290, 1527, 1652, 1676, 1833
MaeII	ACGT	4 cut(s)	138, 1129, 2065, 2364
MalI	GATC	8 cut(s)	214, 1454, 1554, 1674, 1740, 2103, 2247, 2385
MboI	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
MfeI	CAATTG	2 cut(s)	643, 711
MflI	RGATCY	3 cut(s)	1672, 1738, 2383
MhlI	GDGCHC	4 cut(s)	122, 451, 2178, 2561
MluCI	AATT	8 cut(s)	249, 643, 711, 887, 1009, 1077, 1312, 1913
Mly113I	GGCGCC	1 cut(s)	2273
MlyI	GAGTC	4 cut(s)	335, 984, 1942, 2422
Mph1103I	ATGCAT	2 cut(s)	701, 1684
MroXI	GAANNNNTTC	3 cut(s)	249, 857, 1634
MseI	TTAA	8 cut(s)	165, 465, 731, 1014, 1617, 1811, 2228, 2279
MspI	CCGG	4 cut(s)	228, 836, 927, 2312
MspR9I	CCNGG	5 cut(s)	57, 387, 617, 927, 1817
MunI	CAATTG	2 cut(s)	643, 711
Mva1269I	GAATGC	4 cut(s)	1482, 1682, 2017, 2462
MvaI	CCWGG	4 cut(s)	57, 387, 617, 1817
MvnI	CGCG	1 cut(s)	1704
MwoI	GCNNNNNNNGC	5 cut(s)	521, 530, 679, 841, 2351
NarI	GGCGCC	1 cut(s)	2273
NciI	CCSGG	1 cut(s)	927
NdeII	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
NlaIII	CATG	7 cut(s)	176, 356, 673, 699, 1468, 1544, 2557
NlaIV	GGNNCC	8 cut(s)	120, 667, 1100, 1372, 1740, 2198, 2274, 2441
NmeAIII	GCCGAG	1 cut(s)	1097
NmuCI	GTSAC	7 cut(s)	280, 457, 691, 1130, 1882, 2337, 2446
NsiI	ATGCAT	2 cut(s)	701, 1684
NspI	RCATGY	2 cut(s)	699, 1468
PaqCI	CACCTGC	1 cut(s)	1559
PciI	ACATGT	1 cut(s)	1464
PcsI	WCGNNNNNNNCGW	1 cut(s)	1619
PctI	GAATGC	4 cut(s)	1482, 1682, 2017, 2462
PdmI	GAANNNNTTC	3 cut(s)	249, 857, 1634
PfeI	GAWTC	7 cut(s)	177, 203, 608, 719, 858, 950, 2534
PflFI	GACNNNGTC	1 cut(s)	2261
PinAI	ACCGGT	1 cut(s)	227
PkrI	GCNGC	5 cut(s)	82, 641, 843, 1344, 1696
PleI	GAGTC	4 cut(s)	334, 984, 1941, 2422
PluTI	GGCGCC	1 cut(s)	2276
PmaCI	CACGTG	1 cut(s)	1130
PmlI	CACGTG	1 cut(s)	1130
PpsI	GAGTC	4 cut(s)	334, 984, 1941, 2422
Ppu21I	YACGTR	2 cut(s)	1130, 2066
PpuMI	RGGWCCY	1 cut(s)	1099
PscI	ACATGT	1 cut(s)	1464
PshAI	GACNNNNGTC	1 cut(s)	2307
PshBI	ATTAAT	1 cut(s)	2228
PsiI	TTATAA	1 cut(s)	2003
Psp124BI	GAGCTC	3 cut(s)	451, 2178, 2561
Psp5II	RGGWCCY	1 cut(s)	1099
Psp6I	CCWGG	4 cut(s)	55, 385, 615, 1815
PspCI	CACGTG	1 cut(s)	1130
PspEI	GGTNACC	1 cut(s)	217
PspFI	CCCAGC	1 cut(s)	2346
PspGI	CCWGG	4 cut(s)	55, 385, 615, 1815
PspN4I	GGNNCC	8 cut(s)	120, 667, 1100, 1372, 1740, 2198, 2274, 2441
PspOMI	GGGCCC	1 cut(s)	118
PspPI	GGNCC	9 cut(s)	118, 119, 230, 1099, 1274, 1371, 2344, 2440, 2525
PspPPI	RGGWCCY	1 cut(s)	1099
PsrI	GAACNNNNNNTAC	2 cut(s)	1848, 1880
PstI	CTGCAG	3 cut(s)	190, 641, 1437
PstNI	CAGNNNCTG	2 cut(s)	1342, 2341
PsuI	RGATCY	3 cut(s)	1672, 1738, 2383
PsyI	GACNNNGTC	1 cut(s)	2261
SacI	GAGCTC	3 cut(s)	451, 2178, 2561
SaqAI	TTAA	8 cut(s)	165, 465, 731, 1014, 1617, 1811, 2228, 2279
SatI	GCNGC	5 cut(s)	81, 640, 842, 1343, 1695
Sau3AI	GATC	8 cut(s)	212, 1452, 1552, 1672, 1738, 2101, 2245, 2383
Sau96I	GGNCC	9 cut(s)	118, 119, 230, 1099, 1274, 1371, 2344, 2440, 2525
ScaI	AGTACT	2 cut(s)	635, 1805
SchI	GAGTC	4 cut(s)	335, 984, 1942, 2422
ScrFI	CCNGG	5 cut(s)	57, 387, 617, 927, 1817
SduI	GDGCHC	4 cut(s)	122, 451, 2178, 2561
SexAI	ACCWGGT	1 cut(s)	55
SfaNI	GCATC	6 cut(s)	36, 67, 490, 616, 1024, 1669
SfcI	CTRYAG	5 cut(s)	99, 186, 471, 637, 1433
SfoI	GGCGCC	1 cut(s)	2274
SinI	GGWCC	6 cut(s)	230, 1099, 1274, 1371, 2440, 2525
SmlI	CTYRAG	1 cut(s)	1887
SmoI	CTYRAG	1 cut(s)	1887
Sse9I	AATT	8 cut(s)	249, 643, 711, 887, 1009, 1077, 1312, 1913
SsiI	CCGC	3 cut(s)	727, 1979, 2523
SspDI	GGCGCC	1 cut(s)	2272
SspI	AATATT	1 cut(s)	1201
SspMI	CTAG	6 cut(s)	144, 1290, 1527, 1652, 1676, 1833
SstI	GAGCTC	3 cut(s)	451, 2178, 2561
StyD4I	CCNGG	5 cut(s)	55, 385, 615, 925, 1815
StyI	CCWWGG	4 cut(s)	143, 305, 911, 1351
TaaI	ACNGT	6 cut(s)	472, 747, 1262, 1306, 2452, 2546
TaiI	ACGT	4 cut(s)	141, 1132, 2068, 2367
TaqI	TCGA	3 cut(s)	150, 1613, 2454
TaqII	GACCGA	1 cut(s)	2457
TasI	AATT	8 cut(s)	249, 643, 711, 887, 1009, 1077, 1312, 1913
TatI	WGTACW	5 cut(s)	633, 1467, 1803, 2469, 2546
TfiI	GAWTC	7 cut(s)	177, 203, 608, 719, 858, 950, 2534
Tru1I	TTAA	8 cut(s)	165, 465, 731, 1014, 1617, 1811, 2228, 2279
Tru9I	TTAA	8 cut(s)	165, 465, 731, 1014, 1617, 1811, 2228, 2279
TscAI	CASTG	4 cut(s)	1057, 1309, 2140, 2346
TseFI	GTSAC	7 cut(s)	280, 457, 691, 1130, 1882, 2337, 2446
TseI	GCWGC	5 cut(s)	80, 639, 841, 1342, 1694
Tsp45I	GTSAC	7 cut(s)	280, 457, 691, 1130, 1882, 2337, 2446
TspGWI	ACGGA	4 cut(s)	319, 1173, 1945, 1970
TspRI	CASTG	4 cut(s)	1057, 1309, 2140, 2346
Tth111I	GACNNNGTC	1 cut(s)	2261
VpaK11BI	GGWCC	6 cut(s)	230, 1099, 1274, 1371, 2440, 2525
VspI	ATTAAT	1 cut(s)	2228
XapI	RAATTY	2 cut(s)	1009, 1913
XbaI	TCTAGA	2 cut(s)	1651, 1675
XceI	RCATGY	2 cut(s)	699, 1468
XmaJI	CCTAGG	1 cut(s)	143
XmiI	GTMKAC	2 cut(s)	1059, 1608
XmnI	GAANNNNTTC	3 cut(s)	249, 857, 1634
XspI	CTAG	6 cut(s)	144, 1290, 1527, 1652, 1676, 1833
ZrmI	AGTACT	2 cut(s)	635, 1805
Zsp2I	ATGCAT	2 cut(s)	701, 1684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.