Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002175

Physical Location & Seq

Genomic Coordinates Reverse (-)

41450035 .. 41456925

Gene Structure 6891 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002900

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2577 bp

ATGGAGCAAATGGAGGGAGAGAGCTGGAGGGAATTGGTGAAGAAGATGCTCCCACCGGGTGCTTCTCTCCCTGATGATGCTTCTGATCTTGATTACTCCATTGCTATGGAGTATGAGGGCCCTCCTGTCGGTTATGATGTACCTAGGGTTGAACCTCTTGATGTTAACTCACGTGCAATCCCAACTGCAGAACCGCTATCTGAATCTCAAAGATCGGTTACCAATATGGGTCCGCCGGTAATTGAACCAATTCCTCTGCCAATGTCTCGTATTGCTGGTGTCACAAGTTCCCCTACTCAAAGTCCAAGGGTATCAGGAAGCTCAGAATCTGTAGTGTCGGTCTTGCAAAACCCTGACTTTTCTTCAGCATCACCTTCAGCTTCTCCAGGTTCAGTTCATAATCCTCCAAGTGTTCCACCCAAACAAGAAGTGAAGCGAGCTCCAGTGGTCACTTTCAATACTGTTGATAGGCCTCAGAAGAAAGAGGCTGATGTAGAGAAGCCAGTTTTTCCACCTTATGTTGCGGTTTCAAGGGACAAGAAGAAGAAAAAAAGTAGAGTTTGTTACCGGTGCCAAAAAGGAAAGTGGGAAACTAAGGAGTCGTGCCTGGTTTGTGATGCCAAGTATTGCAGCAATTGTGTGCTTCGGGCAATGGGTTCAATGCCTGAAGGGCGTAAGTGTGTGACATGCATTGGTCAGCCAATTGATGAATCAAAGCGGCTGAAATTGGGTAAACAGTCGAGGGTATTGTCCAGATTGCTCAGTCCCTTGGAAGTCAAGCAGATAATGAAAGCAGAGAAAGAATGTGCTGCCAATCAGCTACGGCCAGAGCAACTGATAGTAAATGGATTTCCATTAAAACCAGAAGAGATGGCTGAATTACTTGGATGTGCTTTACCTCCGCGGAAGTTGAAGCCTGGTAGATATTGGTATGACAAAGAATCTGGTCTCTGGGGAAAGGAGGGAGAAAAACCAGATAGAATCATAAGTTCAAACTTAAATTTTACTGGGAAACTTAGCTCTGAAGCAAGCAACGGAAACACTGAAGTTTATATGAATGGTCGAGAAATTACAAAACTTGAGTTGAGGGTGCTTAAGGTGGCACATGTGCAATGTCCACGTGACACCCATTTTTGGGTCTATGATGATGGCCGTTATGAGGAAGAAGGTCAGAACAATATCAGGGGAAACATTTGGGAGAAGGCTACAACTCGGTTTGTCTGTAGCCTGTTCTCTTTGCCCGTGCCACATGGTCAACCTCATGGACCAAGAGATGAGGCGAGCAATTATACCACCGTTCCAAATTATTTGGAGCAGAAAAAAATTCAGAAGCTTCTTCTTCTTGGCCTTCCAGGGTCTGGAACAAGCACTATCTTCAAGCAGGCAAAGTTCTTGTATGGGAACAAATTTACTGCAGAAGAGGTGCAGGATATAAAGTTGATGATCCAAAGTAACATGTACAAATATCTAAGCATTCTGCTAGATGGAAGGGAGCGCTTTGAAGAGGAGGCTTTATCTAGGATGGGCGCACATGGTTCCCATGATCAAAACAAAGCAACAGGTGGTGAGGTTGATAGTGATGAAACTAGTCAATGTATCTACTCCATCAACCCAAGGTTGAAGCATTTCTCTGATTGGCTTCTGGACATTATTGCTACTGGAGATTTAGATGCTTTTTTCCCTGCTGCAACACGCGAGTATGCTCCATTAGTTGAAGAGGTGTGGAAGGATCCTGCTATTCAGGAAACATATAAAAGAAAAGATGAGCTTCATTTCCTCCCAGAGGTTGCCGAATACTTTTTAAGCCAGGCTGTTGAGGTTTCTAGCAATGAGTATGAACCTTCAGAACGAGACATACTATATGCAGAAGGGGTCACTCAAGGAAATGGCTTGGCTTTCATTGAATTCTCTCTTGATGATCGAAGTCCAATGTCTGAAACCTATACAGAAAATCTGGAAGCTCCACCACCGCCGCTAACCAAATACCAACTTATAAGACTTAATGCCAAGGGAATGAATGAAGGGTGTAAGTGGGTGGAAATGTTTGAAGACGTGCGTGTGGTGGTGTTTTGCATCGCTCTTAGTGATTATGATCAGATATGGGTTTCCCCAGACAGTAGTGGGAGCGGATCATTGCTTCAAAATAAGATAATACAAAGCAGGGAGCTATTTGAGACTATGGTCAGACACCCTTGCTTTAAAGATACTCCTTTTGTTTTGGTACTAAACAAGTATGATCTCTTTGAGGACAAGGTTAATCAGGTGCCTTTAAGTACTTGTGAATGGTTCTATGATTTTAGTCCAGTGAAGCCTCACCACAGTAACCAGTCACTGGCCCATCAAGCTTACTTCTATGTTGCAATGAAGTTCAAAGACCTTTATACTTCTATCACGGGTCGAAAGCTTTTCGTGTGGCAAGCTCGGGCAAGGGACCCAGTTACAGTTGATGAGGGATTCAAGTACATAAGAGAGGTTCTCAAGTGGGATGAGGAGAAGGAGGCGAACTACTATGGTGGACCGGAGGATTCATTTTACAGTACAGATATGAGCTCCTCGCCATATGTCAGGCAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009723 GO:0009725 GO:0009898 GO:0009987 GO:0010033 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

859

Amino Acids

97.07

Weight (kDa)

5.49

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	438 - 825	1.8e-71	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930
fragaria_vesca	FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320
malus_domestica	MD06G1025000.v1.1
prunus_persica	Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1
pyrus_communis	pycom06g02030
rosa_chinensis	RchiOBHm_Chr7g0212271
rosa_laevigata	RLG00000002900
rosa_multiflora	Rmu_sc0001911.1_g000047
rosa_roxburghii	Rroxscaffold_3G00246940
rosa_rugosa	Rorug07G0133000 Rorug07G0133100 Rorug07G0133200 Rorug07G0133200
rosa_samantha	Rh7AG265800 Rh7BG260000
rosa_wichuraiana	Rw7G022760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1994
AccB1I	GGYRCC	2 cut(s)	570, 2263
AccB7I	CCANNNNNTGG	2 cut(s)	1358, 2332
AccBSI	CCGCTC	1 cut(s)	2127
AccII	CGCG	2 cut(s)	904, 1695
AciI	CCGC	9 cut(s)	194, 233, 524, 718, 902, 904, 1970, 1973, 2127
AclWI	GGATC	4 cut(s)	1438, 1724, 1737, 2137
AcoI	YGGCCR	2 cut(s)	824, 1150
AcsI	RAATTY	4 cut(s)	1000, 1323, 1406, 1904
AcuI	CTGAAG	6 cut(s)	348, 360, 687, 1044, 1065, 1827
AcvI	CACGTG	2 cut(s)	173, 1121
AdeI	CACNNNGTG	1 cut(s)	59
AfaI	GTAC	6 cut(s)	141, 1460, 2223, 2275, 2462, 2539
AfeI	AGCGCT	1 cut(s)	1496
AfiI	CCNNNNNNNGG	7 cut(s)	128, 1134, 1135, 1159, 1358, 1783, 2332
AflII	CTTAAG	1 cut(s)	1094
AflIII	ACRYGT	2 cut(s)	1105, 1455
AgeI	ACCGGT	1 cut(s)	567
AhlI	ACTAGT	1 cut(s)	1586
AjiI	CACGTC	1 cut(s)	2053
AjnI	CCWGG	5 cut(s)	385, 606, 916, 1351, 1806
Alw21I	GWGCWC	2 cut(s)	442, 2552
Alw26I	GTCTC	4 cut(s)	270, 953, 1845, 2168
AlwI	GGATC	4 cut(s)	1438, 1724, 1737, 2137
AlwNI	CAGNNNCTG	3 cut(s)	329, 1358, 2332
Ama87I	CYCGRG	1 cut(s)	2421
Aor51HI	AGCGCT	1 cut(s)	1496
AoxI	GGCC	6 cut(s)	118, 470, 824, 1150, 1345, 2334
ApaI	GGGCCC	1 cut(s)	122
ApeKI	GCWGC	3 cut(s)	630, 809, 1685
ApoI	RAATTY	4 cut(s)	1000, 1323, 1406, 1904
AsiGI	ACCGGT	1 cut(s)	567
Asp700I	GAANNNNTTC	2 cut(s)	249, 1625
AspA2I	CCTAGG	1 cut(s)	143
AspLEI	GCGC	2 cut(s)	1497, 1529
AspS9I	GGNCC	7 cut(s)	118, 119, 230, 1265, 2335, 2431, 2516
AsuC2I	CCSGG	1 cut(s)	57
AsuHPI	GGTGA	4 cut(s)	49, 363, 1577, 2306
AvaI	CYCGRG	1 cut(s)	2421
AvaII	GGWCC	4 cut(s)	230, 1265, 2431, 2516
AvrII	CCTAGG	1 cut(s)	143
BaeGI	GKGCMC	1 cut(s)	122
BamHI	GGATCC	1 cut(s)	1729
BanI	GGYRCC	2 cut(s)	570, 2263
BanII	GRGCYC	3 cut(s)	122, 442, 2552
BbrPI	CACGTG	2 cut(s)	173, 1121
BbsI	GAAGAC	1 cut(s)	2055
Bbv12I	GWGCWC	2 cut(s)	442, 2552
BbvI	GCAGC	3 cut(s)	642, 796, 1672
BccI	CCATC	6 cut(s)	865, 1142, 1478, 1516, 1613, 2346
BceAI	ACGGC	2 cut(s)	839, 1137
BciT130I	CCWGG	5 cut(s)	387, 608, 918, 1353, 1808
BclI	TGATCA	2 cut(s)	1543, 2092
BcnI	CCSGG	1 cut(s)	57
BcoDI	GTCTC	4 cut(s)	270, 953, 1845, 2168
BcuI	ACTAGT	1 cut(s)	1586
BfaI	CTAG	5 cut(s)	144, 1481, 1518, 1587, 1824
BfmI	CTRYAG	4 cut(s)	186, 330, 1222, 1413
BfoI	RGCGCY	1 cut(s)	1498
BfrI	CTTAAG	1 cut(s)	1094
BglI	GCCNNNNNGGC	1 cut(s)	670
BisI	GCNGC	5 cut(s)	631, 719, 810, 1686, 1973
BlnI	CCTAGG	1 cut(s)	143
BlsI	GCNGC	5 cut(s)	632, 720, 811, 1687, 1974
BmcAI	AGTACT	1 cut(s)	2275
Bme1390I	CCNGG	6 cut(s)	57, 387, 608, 918, 1353, 1808
Bme18I	GGWCC	4 cut(s)	230, 1265, 2431, 2516
BmeT110I	CYCGRG	1 cut(s)	2421
BmgBI	CACGTC	1 cut(s)	2053
BmgT120I	GGNCC	7 cut(s)	118, 119, 230, 1265, 2335, 2431, 2516
BmiI	GGNNCC	8 cut(s)	120, 231, 572, 1537, 1731, 2265, 2432, 2433
BmrFI	CCNGG	6 cut(s)	57, 387, 608, 918, 1353, 1808
BmrI	ACTGGG	2 cut(s)	1017, 2429
BmsI	GCATC	6 cut(s)	36, 67, 377, 607, 1660, 2082
BmuI	ACTGGG	2 cut(s)	1017, 2429
BoxI	GACNNNNGTC	1 cut(s)	2180
BpiI	GAAGAC	1 cut(s)	2055
BplI	GAGNNNNNCTC	2 cut(s)	2460, 2492
BpmI	CTGGAG	4 cut(s)	46, 369, 426, 1680
BpuEI	CTTGAG	3 cut(s)	1100, 1863, 2462
BpuMI	CCSGG	1 cut(s)	57
BsaAI	YACGTR	2 cut(s)	173, 1121
BsaBI	GATNNNNATC	1 cut(s)	2091
BsaI	GGTCTC	1 cut(s)	953
BsaJI	CCNNGG	7 cut(s)	143, 305, 768, 902, 1352, 1613, 2007
BsaWI	WCCGGW	2 cut(s)	567, 2518
Bsc4I	CCNNNNNNNGG	7 cut(s)	128, 1134, 1135, 1159, 1358, 1783, 2332
Bse118I	RCCGGY	2 cut(s)	235, 567
Bse1I	ACTGG	8 cut(s)	443, 503, 1012, 1663, 2303, 2326, 2337, 2435
Bse3DI	GCAATG	6 cut(s)	99, 657, 1118, 1834, 2132, 2367
Bse8I	GATNNNNATC	1 cut(s)	2091
BseBI	CCWGG	5 cut(s)	387, 608, 918, 1353, 1808
BseDI	CCNNGG	7 cut(s)	143, 305, 768, 902, 1352, 1613, 2007
BseGI	GGATG	3 cut(s)	893, 1527, 2491
BseJI	GATNNNNATC	1 cut(s)	2091
BseLI	CCNNNNNNNGG	7 cut(s)	128, 1134, 1135, 1159, 1358, 1783, 2332
BseMI	GCAATG	6 cut(s)	99, 657, 1118, 1834, 2132, 2367
BseMII	CTCAG	3 cut(s)	336, 488, 775
BseNI	ACTGG	8 cut(s)	443, 503, 1012, 1663, 2303, 2326, 2337, 2435
BseRI	GAGGAG	3 cut(s)	1520, 2504, 2542
BseSI	GKGCMC	1 cut(s)	122
BseXI	GCAGC	3 cut(s)	642, 796, 1672
BsgI	GTGCAG	1 cut(s)	1445
Bsh1236I	CGCG	2 cut(s)	904, 1695
BshFI	GGCC	6 cut(s)	120, 472, 826, 1152, 1347, 2336
BshNI	GGYRCC	2 cut(s)	570, 2263
BshTI	ACCGGT	1 cut(s)	567
BsiHKAI	GWGCWC	2 cut(s)	442, 2552
BsiHKCI	CYCGRG	1 cut(s)	2421
BsiSI	CCGG	4 cut(s)	56, 236, 568, 2519
BslFI	GGGAC	3 cut(s)	548, 750, 2444
BslI	CCNNNNNNNGG	7 cut(s)	128, 1134, 1135, 1159, 1358, 1783, 2332
BsmAI	GTCTC	4 cut(s)	270, 953, 1845, 2168
BsmFI	GGGAC	3 cut(s)	548, 750, 2444
BsmI	GAATGC	1 cut(s)	1473
BsnI	GGCC	6 cut(s)	120, 472, 826, 1152, 1347, 2336
Bso31I	GGTCTC	1 cut(s)	953
BsoBI	CYCGRG	1 cut(s)	2421
Bsp120I	GGGCCC	1 cut(s)	118
Bsp1286I	GDGCHC	3 cut(s)	122, 442, 2552
Bsp1407I	TGTACA	1 cut(s)	1458
Bsp143I	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
BspACI	CCGC	9 cut(s)	194, 233, 524, 718, 902, 904, 1970, 1973, 2127
BspANI	GGCC	6 cut(s)	120, 472, 826, 1152, 1347, 2336
BspCNI	CTCAG	3 cut(s)	335, 487, 774
BspFNI	CGCG	2 cut(s)	904, 1695
BspLI	GGNNCC	8 cut(s)	120, 231, 572, 1537, 1731, 2265, 2432, 2433
BspMAI	CTGCAG	2 cut(s)	190, 1417
BspPI	GGATC	4 cut(s)	1438, 1724, 1737, 2137
BspT107I	GGYRCC	2 cut(s)	570, 2263
BspTI	CTTAAG	1 cut(s)	1094
BspTNI	GGTCTC	1 cut(s)	953
BsrBI	CCGCTC	1 cut(s)	2127
BsrDI	GCAATG	6 cut(s)	99, 657, 1118, 1834, 2132, 2367
BsrFI	RCCGGY	2 cut(s)	235, 567
BsrGI	TGTACA	1 cut(s)	1458
BsrI	ACTGG	8 cut(s)	443, 503, 1012, 1663, 2303, 2326, 2337, 2435
BssAI	RCCGGY	2 cut(s)	235, 567
BssECI	CCNNGG	7 cut(s)	143, 305, 768, 902, 1352, 1613, 2007
BssMI	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
BssT1I	CCWWGG	5 cut(s)	143, 305, 768, 1613, 2007
Bst2UI	CCWGG	5 cut(s)	387, 608, 918, 1353, 1808
Bst4CI	ACNGT	7 cut(s)	463, 738, 1297, 2117, 2321, 2443, 2537
Bst6I	CTCTTC	4 cut(s)	861, 1413, 1497, 1710
BstAFI	CTTAAG	1 cut(s)	1094
BstAUI	TGTACA	1 cut(s)	1458
BstBAI	YACGTR	2 cut(s)	173, 1121
BstC8I	GCNNGC	5 cut(s)	438, 1030, 1282, 1383, 2418
BstDEI	CTNAG	7 cut(s)	322, 474, 594, 761, 1016, 1469, 2081
BstDSI	CCRYGG	1 cut(s)	902
BstEII	GGTNACC	1 cut(s)	217
BstENI	CCTNNNNNAGG	1 cut(s)	1781
BstF5I	GGATG	3 cut(s)	893, 1527, 2491
BstFNI	CGCG	2 cut(s)	904, 1695
BstH2I	RGCGCY	1 cut(s)	1498
BstHHI	GCGC	2 cut(s)	1497, 1529
BstKTI	GATC	9 cut(s)	88, 215, 1446, 1546, 1732, 1921, 2095, 2132, 2239
BstMAI	GTCTC	4 cut(s)	270, 953, 1845, 2168
BstMBI	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
BstMWI	GCNNNNNNNGC	2 cut(s)	670, 2342
BstNI	CCWGG	5 cut(s)	387, 608, 918, 1353, 1808
BstNSI	RCATGY	3 cut(s)	690, 1109, 1459
BstPAI	GACNNNNGTC	1 cut(s)	2180
BstPI	GGTNACC	1 cut(s)	217
BstSCI	CCNGG	6 cut(s)	55, 385, 606, 916, 1351, 1806
BstSFI	CTRYAG	4 cut(s)	186, 330, 1222, 1413
BstSLI	GKGCMC	1 cut(s)	122
BstUI	CGCG	2 cut(s)	904, 1695
BstV1I	GCAGC	3 cut(s)	642, 796, 1672
BstV2I	GAAGAC	1 cut(s)	2055
BstX2I	RGATCY	1 cut(s)	1729
BstXI	CCANNNNNNTGG	1 cut(s)	106
BstYI	RGATCY	1 cut(s)	1729
BsuRI	GGCC	6 cut(s)	120, 472, 826, 1152, 1347, 2336
BtgI	CCRYGG	1 cut(s)	902
BtgZI	GCGATG	1 cut(s)	2059
BtrI	CACGTC	1 cut(s)	2053
BtsCI	GGATG	3 cut(s)	893, 1527, 2491
BtsIMutI	CAGTG	4 cut(s)	450, 1041, 2310, 2330
Cac8I	GCNNGC	5 cut(s)	438, 1030, 1282, 1383, 2418
CaiI	CAGNNNCTG	3 cut(s)	329, 1358, 2332
CfoI	GCGC	2 cut(s)	1497, 1529
Cfr10I	RCCGGY	2 cut(s)	235, 567
Cfr13I	GGNCC	7 cut(s)	118, 119, 230, 1265, 2335, 2431, 2516
Cfr42I	CCGCGG	1 cut(s)	905
Csp6I	GTAC	6 cut(s)	140, 1459, 2222, 2274, 2461, 2538
CspAI	ACCGGT	1 cut(s)	567
CviAII	CATG	7 cut(s)	687, 1106, 1250, 1262, 1456, 1532, 1541
CviQI	GTAC	6 cut(s)	140, 1459, 2222, 2274, 2461, 2538
DdeI	CTNAG	7 cut(s)	322, 474, 594, 761, 1016, 1469, 2081
DpnI	GATC	9 cut(s)	87, 214, 1445, 1545, 1731, 1920, 2094, 2131, 2238
DpnII	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
DraI	TTTAAA	1 cut(s)	2200
DraIII	CACNNNGTG	1 cut(s)	59
EaeI	YGGCCR	2 cut(s)	824, 1150
Eam1104I	CTCTTC	4 cut(s)	861, 1413, 1497, 1710
EarI	CTCTTC	4 cut(s)	861, 1413, 1497, 1710
EciI	GGCGGA	1 cut(s)	222
Ecl136II	GAGCTC	2 cut(s)	440, 2550
Eco130I	CCWWGG	5 cut(s)	143, 305, 768, 1613, 2007
Eco147I	AGGCCT	1 cut(s)	472
Eco24I	GRGCYC	3 cut(s)	122, 442, 2552
Eco31I	GGTCTC	1 cut(s)	953
Eco47I	GGWCC	4 cut(s)	230, 1265, 2431, 2516
Eco47III	AGCGCT	1 cut(s)	1496
Eco53kI	GAGCTC	2 cut(s)	440, 2550
Eco57I	CTGAAG	6 cut(s)	348, 360, 687, 1044, 1065, 1827
Eco72I	CACGTG	2 cut(s)	173, 1121
Eco88I	CYCGRG	1 cut(s)	2421
Eco91I	GGTNACC	1 cut(s)	217
EcoICRI	GAGCTC	2 cut(s)	440, 2550
EcoNI	CCTNNNNNAGG	1 cut(s)	1781
EcoO109I	RGGNCCY	3 cut(s)	118, 119, 2431
EcoO65I	GGTNACC	1 cut(s)	217
EcoRI	GAATTC	1 cut(s)	1904
EcoRII	CCWGG	5 cut(s)	385, 606, 916, 1351, 1806
EcoT14I	CCWWGG	5 cut(s)	143, 305, 768, 1613, 2007
EcoT22I	ATGCAT	1 cut(s)	692
EcoT38I	GRGCYC	3 cut(s)	122, 442, 2552
ErhI	CCWWGG	5 cut(s)	143, 305, 768, 1613, 2007
FaeI	CATG	7 cut(s)	690, 1109, 1253, 1265, 1459, 1535, 1544
FalI	AAGNNNNNCTT	2 cut(s)	1752, 1784
FaqI	GGGAC	3 cut(s)	548, 750, 2444
FatI	CATG	7 cut(s)	686, 1105, 1249, 1261, 1455, 1531, 1540
FauNDI	CATATG	1 cut(s)	2560
FbaI	TGATCA	2 cut(s)	1543, 2092
Fnu4HI	GCNGC	5 cut(s)	631, 719, 810, 1686, 1973
FokI	GGATG	3 cut(s)	900, 1534, 2498
FriOI	GRGCYC	3 cut(s)	122, 442, 2552
Fsp4HI	GCNGC	5 cut(s)	631, 719, 810, 1686, 1973
FspBI	CTAG	5 cut(s)	144, 1481, 1518, 1587, 1824
GlaI	GCGC	2 cut(s)	1496, 1528
GluI	GCNGC	5 cut(s)	631, 719, 810, 1686, 1973
GsuI	CTGGAG	4 cut(s)	46, 369, 426, 1680
HaeII	RGCGCY	1 cut(s)	1498
HaeIII	GGCC	6 cut(s)	120, 472, 826, 1152, 1347, 2336
HapII	CCGG	4 cut(s)	56, 236, 568, 2519
HhaI	GCGC	2 cut(s)	1497, 1529
Hin1II	CATG	7 cut(s)	690, 1109, 1253, 1265, 1459, 1535, 1544
Hin6I	GCGC	2 cut(s)	1495, 1527
HinP1I	GCGC	2 cut(s)	1495, 1527
HincII	GTYRAC	2 cut(s)	166, 1256
HindII	GTYRAC	2 cut(s)	166, 1256
HindIII	AAGCTT	3 cut(s)	1331, 2343, 2402
HinfI	GANTC	8 cut(s)	203, 326, 599, 710, 941, 981, 2454, 2525
HpaI	GTTAAC	1 cut(s)	166
HpaII	CCGG	4 cut(s)	56, 236, 568, 2519
HphI	GGTGA	4 cut(s)	49, 363, 1577, 2306
Hpy166II	GTNNAC	5 cut(s)	166, 734, 1118, 1256, 2516
Hpy8I	GTNNAC	5 cut(s)	166, 734, 1118, 1256, 2516
HpyCH4III	ACNGT	7 cut(s)	463, 738, 1297, 2117, 2321, 2443, 2537
HpyCH4IV	ACGT	3 cut(s)	172, 1120, 2052
HpyF10VI	GCNNNNNNNGC	2 cut(s)	670, 2342
HpyF3I	CTNAG	7 cut(s)	322, 474, 594, 761, 1016, 1469, 2081
HpySE526I	ACGT	3 cut(s)	172, 1120, 2052
Hsp92II	CATG	7 cut(s)	690, 1109, 1253, 1265, 1459, 1535, 1544
HspAI	GCGC	2 cut(s)	1495, 1527
KflI	GGGWCCC	1 cut(s)	2431
Ksp22I	TGATCA	2 cut(s)	1543, 2092
KspAI	GTTAAC	1 cut(s)	166
KspI	CCGCGG	1 cut(s)	905
Kzo9I	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
Lsp1109I	GCAGC	3 cut(s)	642, 796, 1672
LweI	GCATC	6 cut(s)	36, 67, 377, 607, 1660, 2082
MaeI	CTAG	5 cut(s)	144, 1481, 1518, 1587, 1824
MaeII	ACGT	3 cut(s)	172, 1120, 2052
MalI	GATC	9 cut(s)	87, 214, 1445, 1545, 1731, 1920, 2094, 2131, 2238
MbiI	CCGCTC	1 cut(s)	2127
MboI	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
MfeI	CAATTG	2 cut(s)	634, 702
MflI	RGATCY	1 cut(s)	1729
MhlI	GDGCHC	3 cut(s)	122, 442, 2552
MlyI	GAGTC	1 cut(s)	608
Mph1103I	ATGCAT	1 cut(s)	692
MroXI	GAANNNNTTC	2 cut(s)	249, 1625
MseI	TTAA	9 cut(s)	165, 857, 998, 1095, 1802, 2001, 2199, 2256, 2270
MslI	CAYNNNNRTG	1 cut(s)	104
MspA1I	CMGCKG	1 cut(s)	904
MspCI	CTTAAG	1 cut(s)	1094
MspI	CCGG	4 cut(s)	56, 236, 568, 2519
MspR9I	CCNGG	6 cut(s)	57, 387, 608, 918, 1353, 1808
MunI	CAATTG	2 cut(s)	634, 702
Mva1269I	GAATGC	1 cut(s)	1473
MvaI	CCWGG	5 cut(s)	387, 608, 918, 1353, 1808
MvnI	CGCG	2 cut(s)	904, 1695
MwoI	GCNNNNNNNGC	2 cut(s)	670, 2342
NciI	CCSGG	1 cut(s)	57
NdeI	CATATG	1 cut(s)	2560
NdeII	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
NlaIII	CATG	7 cut(s)	690, 1109, 1253, 1265, 1459, 1535, 1544
NlaIV	GGNNCC	8 cut(s)	120, 231, 572, 1537, 1731, 2265, 2432, 2433
NmuCI	GTSAC	6 cut(s)	280, 448, 682, 1121, 1873, 2328
NsiI	ATGCAT	1 cut(s)	692
NspI	RCATGY	3 cut(s)	690, 1109, 1459
PceI	AGGCCT	1 cut(s)	472
PciI	ACATGT	2 cut(s)	1105, 1455
PctI	GAATGC	1 cut(s)	1473
PdmI	GAANNNNTTC	2 cut(s)	249, 1625
PfeI	GAWTC	7 cut(s)	203, 326, 710, 941, 981, 2454, 2525
PflMI	CCANNNNNTGG	2 cut(s)	1358, 2332
PinAI	ACCGGT	1 cut(s)	567
PkrI	GCNGC	5 cut(s)	632, 720, 811, 1687, 1974
PleI	GAGTC	1 cut(s)	607
PmaCI	CACGTG	2 cut(s)	173, 1121
PmlI	CACGTG	2 cut(s)	173, 1121
PpsI	GAGTC	1 cut(s)	607
Ppu21I	YACGTR	2 cut(s)	173, 1121
PpuMI	RGGWCCY	1 cut(s)	2431
PscI	ACATGT	2 cut(s)	1105, 1455
PshAI	GACNNNNGTC	1 cut(s)	2180
PsiI	TTATAA	1 cut(s)	1994
Psp124BI	GAGCTC	2 cut(s)	442, 2552
Psp5II	RGGWCCY	1 cut(s)	2431
Psp6I	CCWGG	5 cut(s)	385, 606, 916, 1351, 1806
PspCI	CACGTG	2 cut(s)	173, 1121
PspEI	GGTNACC	1 cut(s)	217
PspGI	CCWGG	5 cut(s)	385, 606, 916, 1351, 1806
PspN4I	GGNNCC	8 cut(s)	120, 231, 572, 1537, 1731, 2265, 2432, 2433
PspOMI	GGGCCC	1 cut(s)	118
PspPI	GGNCC	7 cut(s)	118, 119, 230, 1265, 2335, 2431, 2516
PspPPI	RGGWCCY	1 cut(s)	2431
PsrI	GAACNNNNNNTAC	2 cut(s)	1839, 1871
PstI	CTGCAG	2 cut(s)	190, 1417
PstNI	CAGNNNCTG	3 cut(s)	329, 1358, 2332
PsuI	RGATCY	1 cut(s)	1729
RsaI	GTAC	6 cut(s)	141, 1460, 2223, 2275, 2462, 2539
RsaNI	GTAC	6 cut(s)	140, 1459, 2222, 2274, 2461, 2538
RseI	CAYNNNNRTG	1 cut(s)	104
SacI	GAGCTC	2 cut(s)	442, 2552
SacII	CCGCGG	1 cut(s)	905
SaqAI	TTAA	9 cut(s)	165, 857, 998, 1095, 1802, 2001, 2199, 2256, 2270
SatI	GCNGC	5 cut(s)	631, 719, 810, 1686, 1973
Sau3AI	GATC	9 cut(s)	85, 212, 1443, 1543, 1729, 1918, 2092, 2129, 2236
Sau96I	GGNCC	7 cut(s)	118, 119, 230, 1265, 2335, 2431, 2516
ScaI	AGTACT	1 cut(s)	2275
SchI	GAGTC	1 cut(s)	608
ScrFI	CCNGG	6 cut(s)	57, 387, 608, 918, 1353, 1808
SduI	GDGCHC	3 cut(s)	122, 442, 2552
SfaNI	GCATC	6 cut(s)	36, 67, 377, 607, 1660, 2082
SfcI	CTRYAG	4 cut(s)	186, 330, 1222, 1413
Sfr303I	CCGCGG	1 cut(s)	905
SgrBI	CCGCGG	1 cut(s)	905
SinI	GGWCC	4 cut(s)	230, 1265, 2431, 2516
SmiMI	CAYNNNNRTG	1 cut(s)	104
SmlI	CTYRAG	4 cut(s)	1079, 1094, 1878, 2477
SmoI	CTYRAG	4 cut(s)	1079, 1094, 1878, 2477
SpeI	ACTAGT	1 cut(s)	1586
SseBI	AGGCCT	1 cut(s)	472
SsiI	CCGC	9 cut(s)	194, 233, 524, 718, 902, 904, 1970, 1973, 2127
SspMI	CTAG	5 cut(s)	144, 1481, 1518, 1587, 1824
SstI	GAGCTC	2 cut(s)	442, 2552
StuI	AGGCCT	1 cut(s)	472
StyD4I	CCNGG	6 cut(s)	55, 385, 606, 916, 1351, 1806
StyI	CCWWGG	5 cut(s)	143, 305, 768, 1613, 2007
TaaI	ACNGT	7 cut(s)	463, 738, 1297, 2117, 2321, 2443, 2537
TaiI	ACGT	3 cut(s)	175, 1123, 2055
TaqI	TCGA	4 cut(s)	740, 1063, 1921, 2398
TaqII	GACCGA	1 cut(s)	328
TatI	WGTACW	4 cut(s)	1458, 2273, 2460, 2537
TauI	GCSGC	2 cut(s)	721, 1975
TfiI	GAWTC	7 cut(s)	203, 326, 710, 941, 981, 2454, 2525
Tru1I	TTAA	9 cut(s)	165, 857, 998, 1095, 1802, 2001, 2199, 2256, 2270
Tru9I	TTAA	9 cut(s)	165, 857, 998, 1095, 1802, 2001, 2199, 2256, 2270
TscAI	CASTG	4 cut(s)	450, 1048, 2310, 2337
TseFI	GTSAC	6 cut(s)	280, 448, 682, 1121, 1873, 2328
TseI	GCWGC	3 cut(s)	630, 809, 1685
Tsp45I	GTSAC	6 cut(s)	280, 448, 682, 1121, 1873, 2328
TspGWI	ACGGA	1 cut(s)	1050
TspRI	CASTG	4 cut(s)	450, 1048, 2310, 2337
Van91I	CCANNNNNTGG	2 cut(s)	1358, 2332
Vha464I	CTTAAG	1 cut(s)	1094
VpaK11BI	GGWCC	4 cut(s)	230, 1265, 2431, 2516
XagI	CCTNNNNNAGG	1 cut(s)	1781
XapI	RAATTY	4 cut(s)	1000, 1323, 1406, 1904
XceI	RCATGY	3 cut(s)	690, 1109, 1459
XmaJI	CCTAGG	1 cut(s)	143
XmnI	GAANNNNTTC	2 cut(s)	249, 1625
XspI	CTAG	5 cut(s)	144, 1481, 1518, 1587, 1824
ZrmI	AGTACT	1 cut(s)	2275
Zsp2I	ATGCAT	1 cut(s)	692

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.