Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002175

Physical Location & Seq

Genomic Coordinates Reverse (-)

40241575 .. 40250343

Gene Structure 8769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00246940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGAGCAAATGGAGGGAGAGAGCTGGAGGGAATTGGTGAAGAAGATGCTCCCACCGGGTGCTTCTCTCCCTGATGATGCTTCTGATCTTGATTACTCCATTGCTATGGAGTATGAGGGCCCTCCTGTCGGTTATGATGTACCTAGGGTTGAACCGCTTGATGTTAACTCACGTGCAATCCCAACTGCAGAACCGCTATCTGAATCTCAAAGATCGGTTACCAATATGGGTCCACCAGTTATTGAACCAATTCCTCTGCCAATGTCTCGTATTGCTGGTGTCACAAGTTCCCCTACTCAAAGTCCAAGGGTATCAGTAAGCTCAGAATCTGTAGTGTCGGTCTTGCAAAACCCTGACTTTTCTTCAGCATCACCTTCAGCTTCTCCAGGTTCAATTCATAACCCTCCAGGTGTTCCACCCAAACAAGTTGCCAGTGAAGTGAAGCGAGCTCCAGTGGTCACTTTCAATACTGTTGATAGGCCTCAGAAGAAAGAGGCTGATGTAGAGAAGCCAGTTTTTCCACCTTATGTTGCGGTTTCAAAGGACAAGAAGAAGAAAAGGAGTAGAGTTTGTTACCGGTGCCGAAAAGGAAAGTGGGAAACTAAGGAGTCGTGCCTGGTTTGTGATGCCAAGTATTGCAGCAATTGTGTGCTTCGGGCAATGGGTTCAATGCCTGAAGGGCGGAAGTGTGTGACATGCATTGGTCAGCCAATTGATGAATCAAAGCGGCTGAAATTGGGTAAACAGTCGAGGGTATTGTCCAGATTGCTCAGTCCCTTGGAAGTCAAGCAGATAATGAAAGCAGAGAAAGAATGTGCTGCCAATCAGCTACGGCCAGAGCAACTGATAGTAAATGGATTTCCATTAAAACCAGAAGAGATGGCTGAATTACTTGGATGCGCTTTACCTCCGCGGAAGTTGAAGCCTGGTAGATATTGGTATGACAAAGAATCTGGTCTCTGGGGAAAGGAGGGAGAAAAACCAGATAGAATCATAAGTTCAAACTTAAACTTTACTGGGAAACTTAGCTCTGAAGCAAGCAACGGAAACACTGAAGTTTATATGAATGGTCGAGAAATTACAAAACTTGAGTTGAAGGTGCTTAGGGTGGCAAGTGTGCAATGTCCGCGTGACACCCATTTTTGGGTCTATGATGATGGCCGTTATGAGGAAGAAGGTCAGAACAATATCAGGGGAAACATTTGGGAGAAGGCTACAACTCGGTTTGTCTGTAGCCTGTTCTCTTTGCCCGTGCCACATGGTCAACCTCATGGACCAAGAGATGAAGCTAGCAATTATACCACCATTCCAAATTATTTGGAGCAGAAAAAAATTCAGAAGCTTCTTCTTCTTGGCCTTCCAGGGTCTGGAACAAGCACTATCTTCAAGCAGGCAAAGTTCTTGTATGGGAACAAATTTACTGCAGAAGAGGTGCAGGATATGAAGTTGATGATCCAAAGTAACATGTACAAATATCTAAGCATTCTGCTAGATGGAAGGGAGCGCTTTGAAGAGGAGGCTTTATCTAGGATGGGCACACATGGTTCCCATGATCAAAACAAGGCAACAGGTGGTGAGGTTGATAGTGATGAAACTAGTCAATGTATCTACTCCATCAACCCAAGGTTGAAGCATTTCTCTGATTGGCTTCTGGACATTATTGCTACCGGAGATTTAGATGCTTTTTTCCCTGCTGCAACACGCGAGTATGCTCCATTAGTTGAAGAGGTGTGGAAGGATCCTGCTATTCAGGAAACATATAAAAGAAAAGATGAGCTTCATTTCCTCCCAGAGGTTGCCGAATACTTTTTAAGCCAGGCTGTTGAGGTATCTAGCAATGAGTATGAACCTTCAGAACGAGACATACTATATGCAGAAGGGGTCACTCAAGGAAATGGCTTGGCTTTCATAGAATTCTCTCTTGATGATCGAAGTCCAATGTCTGAAACCTATACAGAAAATCTGGAAGCTCCACCACCGCCGCTAACCAAATACCAACTTATAAGAGTTAATGCCAAGGGGATGAATGAAGGGTGTAAGTGGGTGGAAATGTTTGAAGACGTGCGTGTGGTGGTGTTTTGCGTCGCTCTTAGTGATTATGATCAGATATGGGTTTCCCCAGAAAGTAGTGGCAGCGGATCATTGCTTCAAAATAAGATTATACAAAGCAGGGAGCTATTTGAGACTATGGTCAGACACCCTTGCTTTAAAGATACTCCTTTTGTTTTGGTACTAAACAAGTATGATCTCTTTGAGGACAAGGTTAATCAGGTGCCTTTAAGTACTTGTGAATGGTTCTATGATTTTAGTCCAGTGAAGCCTCACCACAGTAACCAGTCACTGGCCCATCAAGCTTACTTCTATGTTGCAATGAAGTTCAAAGACCTTTATGCTTCTATCACGAGTCAAAAGCTTTTCGTGTGGCAAGCTCGGGCAAGGGACCGAGTTACAGTTGATGAGGGATTCAAGTACATAAGAGAGGTTCTCAAGTGGGATGAGGAGAAGGAGGCGAACTACTACGGTGGACCGGAGGATTCATTTTACAGTACAGATATGAGCTCCTCGCCATATGTCAGGCAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009723 GO:0009725 GO:0009898 GO:0009987 GO:0010033 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

861

Amino Acids

97.41

Weight (kDa)

5.5

Isoelectric Point (pI)

48.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	442 - 828	1.3e-72	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930 AT1G31930
fragaria_vesca	FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320 FvH4_5g23320
malus_domestica	MD06G1025000.v1.1
prunus_persica	Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1 Prupe.5G026100_v2.0.a1
pyrus_communis	pycom06g02030
rosa_chinensis	RchiOBHm_Chr7g0212271
rosa_laevigata	RLG00000002900
rosa_multiflora	Rmu_sc0001911.1_g000047
rosa_roxburghii	Rroxscaffold_3G00246940
rosa_rugosa	Rorug07G0133000 Rorug07G0133100 Rorug07G0133200 Rorug07G0133200
rosa_samantha	Rh7AG265800 Rh7BG260000
rosa_wichuraiana	Rw7G022760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2003
AccB1I	GGYRCC	2 cut(s)	579, 2272
AccB7I	CCANNNNNTGG	2 cut(s)	1367, 2341
AccII	CGCG	3 cut(s)	913, 1129, 1704
AclWI	GGATC	4 cut(s)	1447, 1733, 1746, 2146
AcoI	YGGCCR	2 cut(s)	833, 1159
AcsI	RAATTY	3 cut(s)	1332, 1415, 1913
AcuI	CTGAAG	6 cut(s)	348, 360, 696, 1053, 1074, 1836
AcvI	CACGTG	1 cut(s)	173
AdeI	CACNNNGTG	1 cut(s)	59
AfaI	GTAC	6 cut(s)	141, 1469, 2232, 2284, 2471, 2548
AfeI	AGCGCT	1 cut(s)	1505
AfiI	CCNNNNNNNGG	7 cut(s)	128, 1143, 1144, 1168, 1367, 1792, 2341
AflIII	ACRYGT	1 cut(s)	1464
AgeI	ACCGGT	1 cut(s)	576
AhlI	ACTAGT	1 cut(s)	1595
AjiI	CACGTC	1 cut(s)	2062
AjnI	CCWGG	6 cut(s)	385, 406, 615, 925, 1360, 1815
Alw21I	GWGCWC	2 cut(s)	451, 2561
Alw26I	GTCTC	4 cut(s)	270, 962, 1854, 2177
AlwI	GGATC	4 cut(s)	1447, 1733, 1746, 2146
AlwNI	CAGNNNCTG	3 cut(s)	329, 1367, 2341
Ama87I	CYCGRG	1 cut(s)	2430
Aor51HI	AGCGCT	1 cut(s)	1505
AoxI	GGCC	6 cut(s)	118, 479, 833, 1159, 1354, 2343
ApaI	GGGCCC	1 cut(s)	122
ApeKI	GCWGC	4 cut(s)	639, 818, 1694, 2133
ApoI	RAATTY	3 cut(s)	1332, 1415, 1913
AsiGI	ACCGGT	1 cut(s)	576
Asp700I	GAANNNNTTC	2 cut(s)	249, 1634
AspA2I	CCTAGG	1 cut(s)	143
AspLEI	GCGC	2 cut(s)	902, 1506
AspS9I	GGNCC	7 cut(s)	118, 119, 230, 1274, 2344, 2440, 2525
AsuC2I	CCSGG	1 cut(s)	57
AsuHPI	GGTGA	4 cut(s)	49, 363, 1586, 2315
AsuNHI	GCTAGC	1 cut(s)	1289
AvaI	CYCGRG	1 cut(s)	2430
AvaII	GGWCC	4 cut(s)	230, 1274, 2440, 2525
AvrII	CCTAGG	1 cut(s)	143
BaeGI	GKGCMC	2 cut(s)	122, 1538
BamHI	GGATCC	1 cut(s)	1738
BanI	GGYRCC	2 cut(s)	579, 2272
BanII	GRGCYC	3 cut(s)	122, 451, 2561
BauI	CACGAG	1 cut(s)	2401
BbrPI	CACGTG	1 cut(s)	173
BbsI	GAAGAC	1 cut(s)	2064
Bbv12I	GWGCWC	2 cut(s)	451, 2561
BbvI	GCAGC	4 cut(s)	651, 805, 1681, 2145
BccI	CCATC	6 cut(s)	874, 1151, 1487, 1525, 1622, 2355
BceAI	ACGGC	2 cut(s)	848, 1146
BciT130I	CCWGG	6 cut(s)	387, 408, 617, 927, 1362, 1817
BclI	TGATCA	2 cut(s)	1552, 2101
BcnI	CCSGG	1 cut(s)	57
BcoDI	GTCTC	4 cut(s)	270, 962, 1854, 2177
BcuI	ACTAGT	1 cut(s)	1595
BfaI	CTAG	6 cut(s)	144, 1290, 1490, 1527, 1596, 1833
BfmI	CTRYAG	4 cut(s)	186, 330, 1231, 1422
BfoI	RGCGCY	1 cut(s)	1507
BglI	GCCNNNNNGGC	1 cut(s)	679
BisI	GCNGC	6 cut(s)	640, 728, 819, 1695, 1982, 2134
BlnI	CCTAGG	1 cut(s)	143
BlsI	GCNGC	6 cut(s)	641, 729, 820, 1696, 1983, 2135
BmcAI	AGTACT	1 cut(s)	2284
Bme1390I	CCNGG	7 cut(s)	57, 387, 408, 617, 927, 1362, 1817
Bme18I	GGWCC	4 cut(s)	230, 1274, 2440, 2525
BmeT110I	CYCGRG	1 cut(s)	2430
BmgBI	CACGTC	1 cut(s)	2062
BmgT120I	GGNCC	7 cut(s)	118, 119, 230, 1274, 2344, 2440, 2525
BmiI	GGNNCC	7 cut(s)	120, 231, 581, 1546, 1740, 2274, 2441
BmrFI	CCNGG	7 cut(s)	57, 387, 408, 617, 927, 1362, 1817
BmrI	ACTGGG	1 cut(s)	1026
BmsI	GCATC	6 cut(s)	36, 67, 377, 616, 887, 1669
BmtI	GCTAGC	1 cut(s)	1293
BmuI	ACTGGG	1 cut(s)	1026
BoxI	GACNNNNGTC	1 cut(s)	2189
BpiI	GAAGAC	1 cut(s)	2064
BplI	GAGNNNNNCTC	2 cut(s)	2469, 2501
BpmI	CTGGAG	4 cut(s)	46, 369, 390, 435
Bpu10I	CCTNAGC	1 cut(s)	1103
BpuEI	CTTGAG	3 cut(s)	1109, 1872, 2471
BpuMI	CCSGG	1 cut(s)	57
BsaAI	YACGTR	1 cut(s)	173
BsaBI	GATNNNNATC	1 cut(s)	2100
BsaI	GGTCTC	1 cut(s)	962
BsaJI	CCNNGG	7 cut(s)	143, 305, 777, 911, 1361, 1622, 2016
BsaWI	WCCGGW	3 cut(s)	576, 1667, 2527
Bsc4I	CCNNNNNNNGG	7 cut(s)	128, 1143, 1144, 1168, 1367, 1792, 2341
Bse118I	RCCGGY	1 cut(s)	576
Bse1I	ACTGG	8 cut(s)	236, 432, 452, 512, 1021, 2312, 2335, 2346
Bse3DI	GCAATG	6 cut(s)	99, 666, 1127, 1843, 2141, 2376
Bse8I	GATNNNNATC	1 cut(s)	2100
BseBI	CCWGG	6 cut(s)	387, 408, 617, 927, 1362, 1817
BseDI	CCNNGG	7 cut(s)	143, 305, 777, 911, 1361, 1622, 2016
BseGI	GGATG	4 cut(s)	902, 1536, 2028, 2500
BseJI	GATNNNNATC	1 cut(s)	2100
BseLI	CCNNNNNNNGG	7 cut(s)	128, 1143, 1144, 1168, 1367, 1792, 2341
BseMI	GCAATG	6 cut(s)	99, 666, 1127, 1843, 2141, 2376
BseMII	CTCAG	3 cut(s)	336, 497, 784
BseNI	ACTGG	8 cut(s)	236, 432, 452, 512, 1021, 2312, 2335, 2346
BseRI	GAGGAG	3 cut(s)	1529, 2513, 2551
BseSI	GKGCMC	2 cut(s)	122, 1538
BseXI	GCAGC	4 cut(s)	651, 805, 1681, 2145
BsgI	GTGCAG	1 cut(s)	1454
Bsh1236I	CGCG	3 cut(s)	913, 1129, 1704
BshFI	GGCC	6 cut(s)	120, 481, 835, 1161, 1356, 2345
BshNI	GGYRCC	2 cut(s)	579, 2272
BshTI	ACCGGT	1 cut(s)	576
BsiHKAI	GWGCWC	2 cut(s)	451, 2561
BsiHKCI	CYCGRG	1 cut(s)	2430
BsiSI	CCGG	4 cut(s)	56, 577, 1668, 2528
BslFI	GGGAC	2 cut(s)	759, 2453
BslI	CCNNNNNNNGG	7 cut(s)	128, 1143, 1144, 1168, 1367, 1792, 2341
BsmAI	GTCTC	4 cut(s)	270, 962, 1854, 2177
BsmFI	GGGAC	2 cut(s)	759, 2453
BsmI	GAATGC	1 cut(s)	1482
BsnI	GGCC	6 cut(s)	120, 481, 835, 1161, 1356, 2345
Bso31I	GGTCTC	1 cut(s)	962
BsoBI	CYCGRG	1 cut(s)	2430
Bsp120I	GGGCCC	1 cut(s)	118
Bsp1286I	GDGCHC	4 cut(s)	122, 451, 1538, 2561
Bsp1407I	TGTACA	1 cut(s)	1467
Bsp143I	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
BspANI	GGCC	6 cut(s)	120, 481, 835, 1161, 1356, 2345
BspCNI	CTCAG	3 cut(s)	335, 496, 783
BspFNI	CGCG	3 cut(s)	913, 1129, 1704
BspLI	GGNNCC	7 cut(s)	120, 231, 581, 1546, 1740, 2274, 2441
BspMAI	CTGCAG	2 cut(s)	190, 1426
BspOI	GCTAGC	1 cut(s)	1293
BspPI	GGATC	4 cut(s)	1447, 1733, 1746, 2146
BspT107I	GGYRCC	2 cut(s)	579, 2272
BspTNI	GGTCTC	1 cut(s)	962
BsrDI	GCAATG	6 cut(s)	99, 666, 1127, 1843, 2141, 2376
BsrFI	RCCGGY	1 cut(s)	576
BsrGI	TGTACA	1 cut(s)	1467
BsrI	ACTGG	8 cut(s)	236, 432, 452, 512, 1021, 2312, 2335, 2346
BssAI	RCCGGY	1 cut(s)	576
BssECI	CCNNGG	7 cut(s)	143, 305, 777, 911, 1361, 1622, 2016
BssMI	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
BssSI	CACGAG	1 cut(s)	2401
BssT1I	CCWWGG	5 cut(s)	143, 305, 777, 1622, 2016
Bst2BI	CACGAG	1 cut(s)	2401
Bst2UI	CCWGG	6 cut(s)	387, 408, 617, 927, 1362, 1817
Bst4CI	ACNGT	6 cut(s)	472, 747, 2330, 2452, 2522, 2546
Bst6I	CTCTTC	4 cut(s)	870, 1422, 1506, 1719
BstAUI	TGTACA	1 cut(s)	1467
BstBAI	YACGTR	1 cut(s)	173
BstC8I	GCNNGC	5 cut(s)	447, 1039, 1291, 1392, 2427
BstDEI	CTNAG	8 cut(s)	322, 483, 603, 770, 1025, 1103, 1478, 2090
BstDSI	CCRYGG	1 cut(s)	911
BstEII	GGTNACC	1 cut(s)	217
BstENI	CCTNNNNNAGG	1 cut(s)	1790
BstF5I	GGATG	4 cut(s)	902, 1536, 2028, 2500
BstFNI	CGCG	3 cut(s)	913, 1129, 1704
BstH2I	RGCGCY	1 cut(s)	1507
BstHHI	GCGC	2 cut(s)	902, 1506
BstKTI	GATC	9 cut(s)	88, 215, 1455, 1555, 1741, 1930, 2104, 2141, 2248
BstMAI	GTCTC	4 cut(s)	270, 962, 1854, 2177
BstMBI	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
BstMWI	GCNNNNNNNGC	3 cut(s)	679, 1126, 2351
BstNI	CCWGG	6 cut(s)	387, 408, 617, 927, 1362, 1817
BstNSI	RCATGY	2 cut(s)	699, 1468
BstPAI	GACNNNNGTC	1 cut(s)	2189
BstPI	GGTNACC	1 cut(s)	217
BstSCI	CCNGG	7 cut(s)	55, 385, 406, 615, 925, 1360, 1815
BstSFI	CTRYAG	4 cut(s)	186, 330, 1231, 1422
BstSLI	GKGCMC	2 cut(s)	122, 1538
BstUI	CGCG	3 cut(s)	913, 1129, 1704
BstV1I	GCAGC	4 cut(s)	651, 805, 1681, 2145
BstV2I	GAAGAC	1 cut(s)	2064
BstX2I	RGATCY	1 cut(s)	1738
BstXI	CCANNNNNNTGG	1 cut(s)	106
BstYI	RGATCY	1 cut(s)	1738
BsuRI	GGCC	6 cut(s)	120, 481, 835, 1161, 1356, 2345
BtgI	CCRYGG	1 cut(s)	911
BtrI	CACGTC	1 cut(s)	2062
BtsCI	GGATG	4 cut(s)	902, 1536, 2028, 2500
BtsIMutI	CAGTG	5 cut(s)	439, 459, 1050, 2319, 2339
Cac8I	GCNNGC	5 cut(s)	447, 1039, 1291, 1392, 2427
CaiI	CAGNNNCTG	3 cut(s)	329, 1367, 2341
CfoI	GCGC	2 cut(s)	902, 1506
Cfr10I	RCCGGY	1 cut(s)	576
Cfr13I	GGNCC	7 cut(s)	118, 119, 230, 1274, 2344, 2440, 2525
Cfr42I	CCGCGG	1 cut(s)	914
CseI	GACGC	1 cut(s)	2071
Csp6I	GTAC	6 cut(s)	140, 1468, 2231, 2283, 2470, 2547
CspAI	ACCGGT	1 cut(s)	576
CviAII	CATG	6 cut(s)	696, 1259, 1271, 1465, 1541, 1550
CviQI	GTAC	6 cut(s)	140, 1468, 2231, 2283, 2470, 2547
DdeI	CTNAG	8 cut(s)	322, 483, 603, 770, 1025, 1103, 1478, 2090
DpnI	GATC	9 cut(s)	87, 214, 1454, 1554, 1740, 1929, 2103, 2140, 2247
DpnII	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
DraI	TTTAAA	1 cut(s)	2209
DraIII	CACNNNGTG	1 cut(s)	59
EaeI	YGGCCR	2 cut(s)	833, 1159
Eam1104I	CTCTTC	4 cut(s)	870, 1422, 1506, 1719
EarI	CTCTTC	4 cut(s)	870, 1422, 1506, 1719
EciI	GGCGGA	1 cut(s)	697
Ecl136II	GAGCTC	2 cut(s)	449, 2559
Eco130I	CCWWGG	5 cut(s)	143, 305, 777, 1622, 2016
Eco147I	AGGCCT	1 cut(s)	481
Eco24I	GRGCYC	3 cut(s)	122, 451, 2561
Eco31I	GGTCTC	1 cut(s)	962
Eco47I	GGWCC	4 cut(s)	230, 1274, 2440, 2525
Eco47III	AGCGCT	1 cut(s)	1505
Eco53kI	GAGCTC	2 cut(s)	449, 2559
Eco57I	CTGAAG	6 cut(s)	348, 360, 696, 1053, 1074, 1836
Eco72I	CACGTG	1 cut(s)	173
Eco88I	CYCGRG	1 cut(s)	2430
Eco91I	GGTNACC	1 cut(s)	217
EcoICRI	GAGCTC	2 cut(s)	449, 2559
EcoNI	CCTNNNNNAGG	1 cut(s)	1790
EcoO109I	RGGNCCY	2 cut(s)	118, 119
EcoO65I	GGTNACC	1 cut(s)	217
EcoRI	GAATTC	1 cut(s)	1913
EcoRII	CCWGG	6 cut(s)	385, 406, 615, 925, 1360, 1815
EcoT14I	CCWWGG	5 cut(s)	143, 305, 777, 1622, 2016
EcoT22I	ATGCAT	1 cut(s)	701
EcoT38I	GRGCYC	3 cut(s)	122, 451, 2561
ErhI	CCWWGG	5 cut(s)	143, 305, 777, 1622, 2016
FaeI	CATG	6 cut(s)	699, 1262, 1274, 1468, 1544, 1553
FalI	AAGNNNNNCTT	2 cut(s)	1761, 1793
FaqI	GGGAC	2 cut(s)	759, 2453
FatI	CATG	6 cut(s)	695, 1258, 1270, 1464, 1540, 1549
FauNDI	CATATG	1 cut(s)	2569
FbaI	TGATCA	2 cut(s)	1552, 2101
Fnu4HI	GCNGC	6 cut(s)	640, 728, 819, 1695, 1982, 2134
FokI	GGATG	4 cut(s)	909, 1543, 2035, 2507
FriOI	GRGCYC	3 cut(s)	122, 451, 2561
Fsp4HI	GCNGC	6 cut(s)	640, 728, 819, 1695, 1982, 2134
FspBI	CTAG	6 cut(s)	144, 1290, 1490, 1527, 1596, 1833
GlaI	GCGC	2 cut(s)	901, 1505
GluI	GCNGC	6 cut(s)	640, 728, 819, 1695, 1982, 2134
GsuI	CTGGAG	4 cut(s)	46, 369, 390, 435
HaeII	RGCGCY	1 cut(s)	1507
HaeIII	GGCC	6 cut(s)	120, 481, 835, 1161, 1356, 2345
HapII	CCGG	4 cut(s)	56, 577, 1668, 2528
HgaI	GACGC	1 cut(s)	2071
HhaI	GCGC	2 cut(s)	902, 1506
Hin1II	CATG	6 cut(s)	699, 1262, 1274, 1468, 1544, 1553
Hin6I	GCGC	2 cut(s)	900, 1504
HinP1I	GCGC	2 cut(s)	900, 1504
HincII	GTYRAC	2 cut(s)	166, 1265
HindII	GTYRAC	2 cut(s)	166, 1265
HindIII	AAGCTT	3 cut(s)	1340, 2352, 2411
HinfI	GANTC	9 cut(s)	203, 326, 608, 719, 950, 990, 2404, 2463, 2534
HpaI	GTTAAC	1 cut(s)	166
HpaII	CCGG	4 cut(s)	56, 577, 1668, 2528
HphI	GGTGA	4 cut(s)	49, 363, 1586, 2315
Hpy166II	GTNNAC	5 cut(s)	166, 233, 743, 1265, 2525
Hpy188III	TCNNGA	9 cut(s)	89, 762, 1073, 1368, 1652, 1751, 1922, 1964, 2401
Hpy8I	GTNNAC	5 cut(s)	166, 233, 743, 1265, 2525
Hpy99I	CGWCG	1 cut(s)	2087
HpyCH4III	ACNGT	6 cut(s)	472, 747, 2330, 2452, 2522, 2546
HpyCH4IV	ACGT	2 cut(s)	172, 2061
HpyF10VI	GCNNNNNNNGC	3 cut(s)	679, 1126, 2351
HpyF3I	CTNAG	8 cut(s)	322, 483, 603, 770, 1025, 1103, 1478, 2090
HpySE526I	ACGT	2 cut(s)	172, 2061
Hsp92II	CATG	6 cut(s)	699, 1262, 1274, 1468, 1544, 1553
HspAI	GCGC	2 cut(s)	900, 1504
Ksp22I	TGATCA	2 cut(s)	1552, 2101
KspAI	GTTAAC	1 cut(s)	166
KspI	CCGCGG	1 cut(s)	914
Kzo9I	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
LmnI	GCTCC	9 cut(s)	4, 54, 454, 1321, 1501, 1717, 1975, 2173, 2564
Lsp1109I	GCAGC	4 cut(s)	651, 805, 1681, 2145
LweI	GCATC	6 cut(s)	36, 67, 377, 616, 887, 1669
MaeI	CTAG	6 cut(s)	144, 1290, 1490, 1527, 1596, 1833
MaeII	ACGT	2 cut(s)	172, 2061
MalI	GATC	9 cut(s)	87, 214, 1454, 1554, 1740, 1929, 2103, 2140, 2247
MboI	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
MfeI	CAATTG	2 cut(s)	643, 711
MflI	RGATCY	1 cut(s)	1738
MhlI	GDGCHC	4 cut(s)	122, 451, 1538, 2561
MlyI	GAGTC	2 cut(s)	617, 2413
Mph1103I	ATGCAT	1 cut(s)	701
MroXI	GAANNNNTTC	2 cut(s)	249, 1634
MseI	TTAA	8 cut(s)	165, 866, 1007, 1811, 2010, 2208, 2265, 2279
MslI	CAYNNNNRTG	1 cut(s)	104
MspA1I	CMGCKG	2 cut(s)	913, 2136
MspI	CCGG	4 cut(s)	56, 577, 1668, 2528
MspR9I	CCNGG	7 cut(s)	57, 387, 408, 617, 927, 1362, 1817
MunI	CAATTG	2 cut(s)	643, 711
Mva1269I	GAATGC	1 cut(s)	1482
MvaI	CCWGG	6 cut(s)	387, 408, 617, 927, 1362, 1817
MvnI	CGCG	3 cut(s)	913, 1129, 1704
MwoI	GCNNNNNNNGC	3 cut(s)	679, 1126, 2351
NciI	CCSGG	1 cut(s)	57
NdeI	CATATG	1 cut(s)	2569
NdeII	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
NheI	GCTAGC	1 cut(s)	1289
NlaIII	CATG	6 cut(s)	699, 1262, 1274, 1468, 1544, 1553
NlaIV	GGNNCC	7 cut(s)	120, 231, 581, 1546, 1740, 2274, 2441
NmuCI	GTSAC	6 cut(s)	280, 457, 691, 1130, 1882, 2337
NsiI	ATGCAT	1 cut(s)	701
NspI	RCATGY	2 cut(s)	699, 1468
PceI	AGGCCT	1 cut(s)	481
PciI	ACATGT	1 cut(s)	1464
PctI	GAATGC	1 cut(s)	1482
PdmI	GAANNNNTTC	2 cut(s)	249, 1634
PfeI	GAWTC	7 cut(s)	203, 326, 719, 950, 990, 2463, 2534
PflMI	CCANNNNNTGG	2 cut(s)	1367, 2341
PinAI	ACCGGT	1 cut(s)	576
PkrI	GCNGC	6 cut(s)	641, 729, 820, 1696, 1983, 2135
PleI	GAGTC	2 cut(s)	616, 2412
PmaCI	CACGTG	1 cut(s)	173
PmlI	CACGTG	1 cut(s)	173
PpsI	GAGTC	2 cut(s)	616, 2412
Ppu21I	YACGTR	1 cut(s)	173
PscI	ACATGT	1 cut(s)	1464
PshAI	GACNNNNGTC	1 cut(s)	2189
PsiI	TTATAA	1 cut(s)	2003
Psp124BI	GAGCTC	2 cut(s)	451, 2561
Psp6I	CCWGG	6 cut(s)	385, 406, 615, 925, 1360, 1815
PspCI	CACGTG	1 cut(s)	173
PspEI	GGTNACC	1 cut(s)	217
PspGI	CCWGG	6 cut(s)	385, 406, 615, 925, 1360, 1815
PspN4I	GGNNCC	7 cut(s)	120, 231, 581, 1546, 1740, 2274, 2441
PspOMI	GGGCCC	1 cut(s)	118
PspPI	GGNCC	7 cut(s)	118, 119, 230, 1274, 2344, 2440, 2525
PsrI	GAACNNNNNNTAC	2 cut(s)	1848, 1880
PstI	CTGCAG	2 cut(s)	190, 1426
PstNI	CAGNNNCTG	3 cut(s)	329, 1367, 2341
PsuI	RGATCY	1 cut(s)	1738
RsaI	GTAC	6 cut(s)	141, 1469, 2232, 2284, 2471, 2548
RsaNI	GTAC	6 cut(s)	140, 1468, 2231, 2283, 2470, 2547
RseI	CAYNNNNRTG	1 cut(s)	104
SacI	GAGCTC	2 cut(s)	451, 2561
SacII	CCGCGG	1 cut(s)	914
SaqAI	TTAA	8 cut(s)	165, 866, 1007, 1811, 2010, 2208, 2265, 2279
SatI	GCNGC	6 cut(s)	640, 728, 819, 1695, 1982, 2134
Sau3AI	GATC	9 cut(s)	85, 212, 1452, 1552, 1738, 1927, 2101, 2138, 2245
Sau96I	GGNCC	7 cut(s)	118, 119, 230, 1274, 2344, 2440, 2525
ScaI	AGTACT	1 cut(s)	2284
SchI	GAGTC	2 cut(s)	617, 2413
ScrFI	CCNGG	7 cut(s)	57, 387, 408, 617, 927, 1362, 1817
SduI	GDGCHC	4 cut(s)	122, 451, 1538, 2561
SfaNI	GCATC	6 cut(s)	36, 67, 377, 616, 887, 1669
SfcI	CTRYAG	4 cut(s)	186, 330, 1231, 1422
Sfr303I	CCGCGG	1 cut(s)	914
SgrBI	CCGCGG	1 cut(s)	914
SinI	GGWCC	4 cut(s)	230, 1274, 2440, 2525
SmiMI	CAYNNNNRTG	1 cut(s)	104
SmlI	CTYRAG	3 cut(s)	1088, 1887, 2486
SmoI	CTYRAG	3 cut(s)	1088, 1887, 2486
SpeI	ACTAGT	1 cut(s)	1595
SseBI	AGGCCT	1 cut(s)	481
SspMI	CTAG	6 cut(s)	144, 1290, 1490, 1527, 1596, 1833
SstI	GAGCTC	2 cut(s)	451, 2561
StuI	AGGCCT	1 cut(s)	481
StyD4I	CCNGG	7 cut(s)	55, 385, 406, 615, 925, 1360, 1815
StyI	CCWWGG	5 cut(s)	143, 305, 777, 1622, 2016
TaaI	ACNGT	6 cut(s)	472, 747, 2330, 2452, 2522, 2546
TaiI	ACGT	2 cut(s)	175, 2064
TaqI	TCGA	3 cut(s)	749, 1072, 1930
TaqII	GACCGA	2 cut(s)	328, 2457
TatI	WGTACW	4 cut(s)	1467, 2282, 2469, 2546
TauI	GCSGC	2 cut(s)	730, 1984
TfiI	GAWTC	7 cut(s)	203, 326, 719, 950, 990, 2463, 2534
Tru1I	TTAA	8 cut(s)	165, 866, 1007, 1811, 2010, 2208, 2265, 2279
Tru9I	TTAA	8 cut(s)	165, 866, 1007, 1811, 2010, 2208, 2265, 2279
TscAI	CASTG	5 cut(s)	439, 459, 1057, 2319, 2346
TseFI	GTSAC	6 cut(s)	280, 457, 691, 1130, 1882, 2337
TseI	GCWGC	4 cut(s)	639, 818, 1694, 2133
Tsp45I	GTSAC	6 cut(s)	280, 457, 691, 1130, 1882, 2337
TspGWI	ACGGA	1 cut(s)	1059
TspRI	CASTG	5 cut(s)	439, 459, 1057, 2319, 2346
Van91I	CCANNNNNTGG	2 cut(s)	1367, 2341
VpaK11BI	GGWCC	4 cut(s)	230, 1274, 2440, 2525
XagI	CCTNNNNNAGG	1 cut(s)	1790
XapI	RAATTY	3 cut(s)	1332, 1415, 1913
XceI	RCATGY	2 cut(s)	699, 1468
XmaJI	CCTAGG	1 cut(s)	143
XmnI	GAANNNNTTC	2 cut(s)	249, 1634
XspI	CTAG	6 cut(s)	144, 1290, 1490, 1527, 1596, 1833
ZrmI	AGTACT	1 cut(s)	2284
Zsp2I	ATGCAT	1 cut(s)	701

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.