FvH4_5g23870

Protein of unknown function (DUF3464)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015437
Physical Location & Seq
Reverse (-)
15159193 .. 15160836
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23870.t1

Sequence Viewer

Length: 639 bp
ATGGCAGCTGCTACAGGTTCATGGTCTTCCAAGCCCTCTCCAATCACTTCGCAACACCCATCAATATTACCTTCTAAGATTGGTCTTAAGCCTTGGACACTGTCCACAAAACCCAGCTATCCCATTTCCAGAACTAAAGAAAACTACTTTCCATCATCCTCCACACAATTAAATGCGACCTTGAAGAACCCTAGAGGCTTCGGACGCTCACCGAAGAAAAACAAGAAGACAACCACCAAGAAGAAGAAGACAGAACAAAACAATGACAGTGGCGATGAAGATGATGAAGACGAAGACGCCGAAGCAGCAGCAGAAGAAGAAGGCATAATACCAGAGATAGTGACCAACAGGATGATAAGCAGAATGGGATTCACAGTTGGTCTCCCATTGTTTACGGGACTATTGTTTTTCCCGTTCTTCTACTACTTGAAAGTTGGCCTCAAACTTGATGTGCCAACATGGGTGCCCTTTATTGTGTCATTTGTTTTCTTTGGGACAGCACTTGCGGGAGTGAGCTATGGCATTGTGTCCTCTAGTTGGGATCCTATGAGGGAAGGGTCACTTTTGGGTTGGAATGAGGCTCAGAAGAATTGGCCTGTATTTTGGAGATCTATTAGGGGTGGATCTAGGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3464

Protein Analysis

213

Amino Acids

23.59

Weight (kDa)

9.54

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 71 - 204 6.5e-50 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19100 AT4G19100
fragaria_vesca FvH4_5g23870
malus_domestica MD06G1014500.v1.1
prunus_persica Prupe.5G021700_v2.0.a1
pyrus_communis pycom06g01160
rosa_chinensis RchiOBHm_Chr7g0213261
rosa_laevigata RLG00000002817
rosa_multiflora Rmu_ssc0000084.1_g000025
rosa_roxburghii Rroxscaffold_3G00246130
rosa_rugosa Rorug07G0140900
rosa_samantha Rh7AG272400 Rh7BG268100 Rh7CG291800 Rh7DG280300
rosa_wichuraiana Rw7G023640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 463
AciI CCGC 1 cut(s) 506
AclWI GGATC 3 cut(s) 536, 549, 631
AcyI GRCGYC 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 537
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 2 cut(s) 184, 430
AluBI AGCT 3 cut(s) 8, 117, 516
AluI AGCT 3 cut(s) 8, 117, 516
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 3 cut(s) 536, 549, 631
AoxI GGCC 2 cut(s) 436, 593
ApeKI GCWGC 4 cut(s) 5, 8, 305, 308
AsuHPI GGTGA 1 cut(s) 201
BaeGI GKGCMC 1 cut(s) 468
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 1 cut(s) 463
BarI GAAGNNNNNNTAC 2 cut(s) 312, 344
BbsI GAAGAC 5 cut(s) 18, 233, 254, 294, 300
BbvI GCAGC 3 cut(s) 17, 317, 320
BccI CCATC 2 cut(s) 67, 160
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 3 cut(s) 192, 534, 627
BfmI CTRYAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 86
BglII AGATCT 1 cut(s) 608
BisI GCNGC 4 cut(s) 6, 9, 306, 309
BlsI GCNGC 4 cut(s) 7, 10, 307, 310
BmiI GGNNCC 2 cut(s) 465, 543
BpiI GAAGAC 5 cut(s) 18, 233, 254, 294, 300
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 92
Bsc4I CCNNNNNNNGG 1 cut(s) 537
BseDI CCNNGG 1 cut(s) 92
BseGI GGATG 2 cut(s) 155, 357
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMII CTCAG 1 cut(s) 596
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 3 cut(s) 17, 317, 320
BseYI CCCAGC 1 cut(s) 113
BshFI GGCC 2 cut(s) 438, 595
BshNI GGYRCC 1 cut(s) 463
BslFI GGGAC 2 cut(s) 411, 508
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 411, 508
BsnI GGCC 2 cut(s) 438, 595
Bso31I GGTCTC 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 3 cut(s) 541, 608, 623
BspACI CCGC 1 cut(s) 506
BspANI GGCC 2 cut(s) 438, 595
BspCNI CTCAG 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 465, 543
BspPI GGATC 3 cut(s) 536, 549, 631
BspT107I GGYRCC 1 cut(s) 463
BspTI CTTAAG 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 386
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 3 cut(s) 541, 608, 623
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 92
Bst4CI ACNGT 3 cut(s) 102, 269, 376
BstACI GRCGYC 1 cut(s) 297
BstAFI CTTAAG 1 cut(s) 86
BstDEI CTNAG 2 cut(s) 75, 582
BstF5I GGATG 2 cut(s) 155, 357
BstKTI GATC 3 cut(s) 544, 611, 626
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 3 cut(s) 541, 608, 623
BstMWI GCNNNNNNNGC 2 cut(s) 204, 305
BstSFI CTRYAG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 468
BstV1I GCAGC 3 cut(s) 17, 317, 320
BstV2I GAAGAC 5 cut(s) 18, 233, 254, 294, 300
BstX2I RGATCY 3 cut(s) 541, 608, 623
BstYI RGATCY 3 cut(s) 541, 608, 623
BsuRI GGCC 2 cut(s) 438, 595
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 155, 357
BtsIMutI CAGTG 2 cut(s) 98, 274
CseI GACGC 2 cut(s) 213, 305
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 2 cut(s) 21, 459
CviJI RGCY 9 cut(s) 8, 34, 91, 117, 198, 438, 516, 581, 595
CviKI_1 RGCY 9 cut(s) 8, 34, 91, 117, 198, 438, 516, 581, 595
DdeI CTNAG 2 cut(s) 75, 582
DpnI GATC 3 cut(s) 543, 610, 625
DpnII GATC 3 cut(s) 541, 608, 623
Eco130I CCWWGG 1 cut(s) 92
Eco31I GGTCTC 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 2 cut(s) 24, 462
FaiI YATR 5 cut(s) 22, 326, 460, 519, 548
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 2 cut(s) 411, 508
FatI CATG 2 cut(s) 20, 458
FauI CCCGC 1 cut(s) 499
Fnu4HI GCNGC 4 cut(s) 6, 9, 306, 309
FokI GGATG 2 cut(s) 142, 364
Fsp4HI GCNGC 4 cut(s) 6, 9, 306, 309
FspBI CTAG 3 cut(s) 192, 534, 627
GluI GCNGC 4 cut(s) 6, 9, 306, 309
GsaI CCCAGC 1 cut(s) 117
HaeIII GGCC 2 cut(s) 438, 595
HgaI GACGC 2 cut(s) 213, 305
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 2 cut(s) 24, 462
HinfI GANTC 1 cut(s) 369
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 2 cut(s) 105, 393
Hpy188I TCNGA 2 cut(s) 203, 585
Hpy188III TCNNGA 1 cut(s) 129
Hpy8I GTNNAC 2 cut(s) 105, 393
HpyAV CCTTC 3 cut(s) 81, 314, 548
HpyCH4III ACNGT 3 cut(s) 102, 269, 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 204, 305
HpyF3I CTNAG 2 cut(s) 75, 582
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 2 cut(s) 24, 462
Kzo9I GATC 3 cut(s) 541, 608, 623
LpnPI CCDG 5 cut(s) 127, 142, 334, 345, 609
Lsp1109I GCAGC 3 cut(s) 17, 317, 320
MaeI CTAG 3 cut(s) 192, 534, 627
MaeIII GTNAC 2 cut(s) 340, 558
MalI GATC 3 cut(s) 543, 610, 625
MboI GATC 3 cut(s) 541, 608, 623
MflI RGATCY 3 cut(s) 541, 608, 623
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 2 cut(s) 167, 589
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 7 cut(s) 46, 169, 188, 449, 541, 543, 571
MseI TTAA 2 cut(s) 87, 170
MspA1I CMGCKG 1 cut(s) 8
MspCI CTTAAG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 204, 305
NdeII GATC 3 cut(s) 541, 608, 623
NlaIII CATG 2 cut(s) 24, 462
NlaIV GGNNCC 2 cut(s) 465, 543
NmuCI GTSAC 2 cut(s) 340, 558
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 1 cut(s) 369
PflFI GACNNNGTC 1 cut(s) 100
PkrI GCNGC 4 cut(s) 7, 10, 307, 310
PspFI CCCAGC 1 cut(s) 113
PspN4I GGNNCC 2 cut(s) 465, 543
PsuI RGATCY 3 cut(s) 541, 608, 623
PsyI GACNNNGTC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 8
SaqAI TTAA 2 cut(s) 87, 170
SatI GCNGC 4 cut(s) 6, 9, 306, 309
Sau3AI GATC 3 cut(s) 541, 608, 623
SduI GDGCHC 1 cut(s) 468
SetI ASST 6 cut(s) 10, 19, 73, 119, 182, 518
SfcI CTRYAG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 2 cut(s) 167, 589
SsiI CCGC 1 cut(s) 506
SspI AATATT 1 cut(s) 66
SspMI CTAG 3 cut(s) 192, 534, 627
StyI CCWWGG 1 cut(s) 92
TaaI ACNGT 3 cut(s) 102, 269, 376
TasI AATT 2 cut(s) 167, 589
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 2 cut(s) 87, 170
Tru9I TTAA 2 cut(s) 87, 170
TscAI CASTG 2 cut(s) 105, 274
TseFI GTSAC 2 cut(s) 340, 558
TseI GCWGC 4 cut(s) 5, 8, 305, 308
Tsp45I GTSAC 2 cut(s) 340, 558
TspDTI ATGAA 3 cut(s) 9, 291, 300
TspRI CASTG 2 cut(s) 105, 274
Tth111I GACNNNGTC 1 cut(s) 100
Vha464I CTTAAG 1 cut(s) 86
XspI CTAG 3 cut(s) 192, 534, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.