MD06G1014500.v1.1

Protein of unknown function (DUF3464)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015437
Physical Location & Seq
Reverse (-)
1841069 .. 1842458
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1014500.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGCTGCAATTGCAGCAGGGCTATCTTCATGGTCATCATCCAAGCCCTCCCTGATTCCTTCCCAGCACCAAATATCATCACCATCATCATCTAATAAGGTTGACGCTAAGCCTTGGAGCCTCTCCATCACCAGCAGTTTAAAAGAAGATATAAAAATCCAGTACCCAATCTCCACAACAAAACAATGCCCAAATACTCAGAAACACTTGAATGCCACCCTGAGAAGCCCAAGAGGCTTTGGACCCTCCCCAAGGAAAAAGAAAAAGATCACCGAGAAGCCGAAGAAGACCCGAAACAATGACAGCGACGATGACGACGACGACGACGACGATGAAGATGAAGATGAGGAGGAAGGAGAGGAAGGAGTGATACCGGAGATCGTGACCAACAGGATGATTGGAAGGATGGGATTCACAGTTGGGCTTCCATTATTTTTGGGCCTCTTGTTTTTCCCTTTCTTTTACTATTTGAAAGTTGGCCTGAAAATTGATGTGCCCACGTGGGTGCCCTTCATTGTGTCATTTGTCTTCTTTGGGACAGCCCTATTGGGGGTCAGCTATGGGATTGTGTCCTCCAGCTGGGATCCTATGAGGGAAGGCTCCCTCTTGGGTTGGACTGAAGCTCAGAAAAATTGGCCTGTCTTTTGGCAGTCCCTTCGTGGTGGTGGATCGAAAAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3464

Protein Analysis

228

Amino Acids

25.24

Weight (kDa)

5.8

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 80 - 218 1.1e-50 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19100 AT4G19100
fragaria_vesca FvH4_5g23870
malus_domestica MD06G1014500.v1.1
prunus_persica Prupe.5G021700_v2.0.a1
pyrus_communis pycom06g01160
rosa_chinensis RchiOBHm_Chr7g0213261
rosa_laevigata RLG00000002817
rosa_multiflora Rmu_ssc0000084.1_g000025
rosa_roxburghii Rroxscaffold_3G00246130
rosa_rugosa Rorug07G0140900
rosa_samantha Rh7AG272400 Rh7BG268100 Rh7CG291800 Rh7DG280300
rosa_wichuraiana Rw7G023640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 505
AclWI GGATC 3 cut(s) 578, 591, 676
AcuI CTGAAG 1 cut(s) 639
AcvI CACGTG 1 cut(s) 501
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 4 cut(s) 252, 549, 550, 579
AgsI TTSAA 2 cut(s) 211, 472
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 3 cut(s) 558, 579, 623
AluI AGCT 3 cut(s) 558, 579, 623
AlwI GGATC 3 cut(s) 578, 591, 676
AoxI GGCC 3 cut(s) 439, 478, 635
ApeKI GCWGC 2 cut(s) 5, 14
AspS9I GGNCC 2 cut(s) 242, 439
AsuHPI GGTGA 3 cut(s) 72, 121, 262
AvaII GGWCC 1 cut(s) 242
BaeGI GKGCMC 2 cut(s) 498, 510
BamHI GGATCC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 505
BarI GAAGNNNNNNTAC 2 cut(s) 354, 386
BbrPI CACGTG 1 cut(s) 501
BbsI GAAGAC 2 cut(s) 293, 520
BbvI GCAGC 1 cut(s) 26
BccI CCATC 3 cut(s) 91, 134, 400
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 2 cut(s) 6, 15
BlpI GCTNAGC 1 cut(s) 108
BlsI GCNGC 2 cut(s) 7, 16
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 242, 439
BmiI GGNNCC 5 cut(s) 119, 244, 507, 585, 601
BpiI GAAGAC 2 cut(s) 293, 520
BpmI CTGGAG 1 cut(s) 559
Bpu1102I GCTNAGC 1 cut(s) 108
BsaAI YACGTR 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 113, 251
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 549, 550, 579
Bse1I ACTGG 1 cut(s) 160
BseDI CCNNGG 2 cut(s) 113, 251
BseGI GGATG 3 cut(s) 38, 399, 411
BseLI CCNNNNNNNGG 4 cut(s) 252, 549, 550, 579
BseMII CTCAG 3 cut(s) 212, 212, 638
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 2 cut(s) 498, 510
BseXI GCAGC 1 cut(s) 26
BseYI CCCAGC 2 cut(s) 63, 579
BshFI GGCC 3 cut(s) 441, 480, 637
BshNI GGYRCC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 374
BslFI GGGAC 2 cut(s) 550, 637
BslI CCNNNNNNNGG 4 cut(s) 252, 549, 550, 579
BsmFI GGGAC 2 cut(s) 550, 637
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 3 cut(s) 441, 480, 637
Bsp1286I GDGCHC 2 cut(s) 498, 510
Bsp143I GATC 4 cut(s) 267, 378, 583, 668
Bsp1720I GCTNAGC 1 cut(s) 108
BspANI GGCC 3 cut(s) 441, 480, 637
BspCNI CTCAG 3 cut(s) 211, 213, 637
BspLI GGNNCC 5 cut(s) 119, 244, 507, 585, 601
BspPI GGATC 3 cut(s) 578, 591, 676
BspT107I GGYRCC 1 cut(s) 505
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 113, 251
BssMI GATC 4 cut(s) 267, 378, 583, 668
BssT1I CCWWGG 2 cut(s) 113, 251
Bst4CI ACNGT 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 501
BstDEI CTNAG 4 cut(s) 108, 198, 221, 624
BstENI CCTNNNNNAGG 1 cut(s) 250
BstF5I GGATG 3 cut(s) 38, 399, 411
BstKTI GATC 4 cut(s) 270, 381, 586, 671
BstMBI GATC 4 cut(s) 267, 378, 583, 668
BstMWI GCNNNNNNNGC 3 cut(s) 11, 14, 234
BstSLI GKGCMC 2 cut(s) 498, 510
BstV1I GCAGC 1 cut(s) 26
BstV2I GAAGAC 2 cut(s) 293, 520
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuRI GGCC 3 cut(s) 441, 480, 637
BtsCI GGATG 3 cut(s) 38, 399, 411
Cfr13I GGNCC 2 cut(s) 242, 439
CseI GACGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 163
CviAII CATG 1 cut(s) 30
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 4 cut(s) 108, 198, 221, 624
DpnI GATC 4 cut(s) 269, 380, 585, 670
DpnII GATC 4 cut(s) 267, 378, 583, 668
DraI TTTAAA 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 113, 251
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 639
Eco72I CACGTG 1 cut(s) 501
EcoNI CCTNNNNNAGG 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 113, 251
ErhI CCWWGG 2 cut(s) 113, 251
FaeI CATG 1 cut(s) 33
FaiI YATR 4 cut(s) 31, 152, 561, 590
FaqI GGGAC 2 cut(s) 550, 637
FatI CATG 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 6, 15
FokI GGATG 3 cut(s) 25, 406, 418
Fsp4HI GCNGC 2 cut(s) 6, 15
GluI GCNGC 2 cut(s) 6, 15
GsaI CCCAGC 2 cut(s) 67, 583
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 3 cut(s) 441, 480, 637
HapII CCGG 1 cut(s) 374
HgaI GACGC 1 cut(s) 113
Hin1II CATG 1 cut(s) 33
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 2 cut(s) 55, 411
HpaII CCGG 1 cut(s) 374
HphI GGTGA 3 cut(s) 72, 121, 262
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 201, 627
Hpy188III TCNNGA 1 cut(s) 382
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 6 cut(s) 311, 320, 323, 326, 329, 332
HpyAV CCTTC 7 cut(s) 69, 347, 356, 396, 520, 590, 665
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 2 cut(s) 8, 14
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 14, 234
HpyF3I CTNAG 4 cut(s) 108, 198, 221, 624
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 1 cut(s) 33
Kzo9I GATC 4 cut(s) 267, 378, 583, 668
LmnI GCTCC 2 cut(s) 117, 605
Lsp1109I GCAGC 1 cut(s) 26
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 382
MalI GATC 4 cut(s) 269, 380, 585, 670
MboI GATC 4 cut(s) 267, 378, 583, 668
MboII GAAGA 7 cut(s) 18, 158, 295, 298, 347, 353, 520
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 2 cut(s) 498, 510
MluCI AATT 3 cut(s) 9, 486, 631
MmeI TCCRAC 1 cut(s) 593
MseI TTAA 1 cut(s) 140
MslI CAYNNNNRTG 2 cut(s) 210, 503
MspA1I CMGCKG 1 cut(s) 579
MspI CCGG 1 cut(s) 374
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 11, 14, 234
NdeII GATC 4 cut(s) 267, 378, 583, 668
NlaIII CATG 1 cut(s) 33
NlaIV GGNNCC 5 cut(s) 119, 244, 507, 585, 601
NmuCI GTSAC 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 503
PcsI WCGNNNNNNNCGW 4 cut(s) 315, 321, 324, 327
PctI GAATGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 55, 411
PkrI GCNGC 2 cut(s) 7, 16
PmaCI CACGTG 1 cut(s) 501
PmlI CACGTG 1 cut(s) 501
Ppu21I YACGTR 1 cut(s) 501
PspCI CACGTG 1 cut(s) 501
PspFI CCCAGC 2 cut(s) 63, 579
PspN4I GGNNCC 5 cut(s) 119, 244, 507, 585, 601
PspPI GGNCC 2 cut(s) 242, 439
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 579
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 2 cut(s) 210, 503
SaqAI TTAA 1 cut(s) 140
SatI GCNGC 2 cut(s) 6, 15
Sau3AI GATC 4 cut(s) 267, 378, 583, 668
Sau96I GGNCC 2 cut(s) 242, 439
SduI GDGCHC 2 cut(s) 498, 510
SetI ASST 5 cut(s) 102, 503, 560, 581, 625
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 210, 503
Sse9I AATT 3 cut(s) 9, 486, 631
StyI CCWWGG 2 cut(s) 113, 251
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 1 cut(s) 503
TaqI TCGA 1 cut(s) 671
TasI AATT 3 cut(s) 9, 486, 631
TfiI GAWTC 2 cut(s) 55, 411
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 2 cut(s) 5, 14
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 4 cut(s) 18, 348, 354, 502
VpaK11BI GGWCC 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.