Rroxscaffold_3G00246130

Protein of unknown function (DUF3464)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015437
Physical Location & Seq
Forward (+)
39221067 .. 39222285
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246130.1

Sequence Viewer

Length: 639 bp
ATGGCAGCTGCAACTGCAGGTTCATGGTCTTCCAAGCCCTCTCCAATCACTTCGCAACACCCATCATTATCATTTAAGATTGGCCTTAAGCCTTGGACGCTGCGCCCAAAACCCAACTACCCCATTTTCAGAACAAATCAAAGCAAAAATCAGAACTACTTTTCATCATCCTCCACACAATTAAATGCGACCTTCAAGAATCCAAGAGGCTTCGGACGGCCACCAAAGAAAAGCAAGAAGACCACCACCAAGAAGAAGACGGAACAAAACAATGACAGTGGCAATGAAGATGAGGAAGACGAAGAGGACGAAGGAGAAGAAGGCATAATACCAGAGATAGTGACCAACAGGATGATAACCAGGATGGGATTCACAGTTGGGCTTCCACTGTTTATGGGATTATTGTTTTTCCCATTCTTCTACTACTTGAAAGTTGGTCTCAAAATTGATGTGCCCACATGGGTGCCCTTCATTGTGTCATTTGTGTTCTTTGGGACAGCTCTTGCGGGAGTGAGCTATGGGATTGTGTCCTCTAGTTGGGACCCTATGAGGGAAGGGTCGCTCTTGGGTTGGAACGAGGCTCAGAAGAATTGGCCTGTATTTTGGAGATCTCTTAGGGGTGGATCGAGGAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3464

Protein Analysis

212

Amino Acids

23.9

Weight (kDa)

9.75

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 69 - 204 6.4e-50 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19100 AT4G19100
fragaria_vesca FvH4_5g23870
malus_domestica MD06G1014500.v1.1
prunus_persica Prupe.5G021700_v2.0.a1
pyrus_communis pycom06g01160
rosa_chinensis RchiOBHm_Chr7g0213261
rosa_laevigata RLG00000002817
rosa_multiflora Rmu_ssc0000084.1_g000025
rosa_roxburghii Rroxscaffold_3G00246130
rosa_rugosa Rorug07G0140900
rosa_samantha Rh7AG272400 Rh7BG268100 Rh7CG291800 Rh7DG280300
rosa_wichuraiana Rw7G023640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 463
AciI CCGC 1 cut(s) 506
AclWI GGATC 1 cut(s) 631
AcoI YGGCCR 1 cut(s) 218
AfiI CCNNNNNNNGG 2 cut(s) 537, 550
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 2 cut(s) 196, 430
AjnI CCWGG 1 cut(s) 359
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 3 cut(s) 8, 500, 516
AluI AGCT 3 cut(s) 8, 500, 516
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 631
AoxI GGCC 3 cut(s) 82, 218, 593
ApeKI GCWGC 3 cut(s) 5, 8, 100
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 541
AvaII GGWCC 1 cut(s) 541
BaeGI GKGCMC 2 cut(s) 456, 468
BanI GGYRCC 1 cut(s) 463
BarI GAAGNNNNNNTAC 2 cut(s) 312, 344
BbsI GAAGAC 4 cut(s) 21, 245, 263, 303
BbvI GCAGC 2 cut(s) 17, 87
BccI CCATC 2 cut(s) 70, 358
BceAI ACGGC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 361
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 534, 637
BfmI CTRYAG 1 cut(s) 15
BfrI CTTAAG 1 cut(s) 86
BfuAI ACCTGC 1 cut(s) 8
BglII AGATCT 1 cut(s) 608
BisI GCNGC 3 cut(s) 6, 9, 101
BlsI GCNGC 3 cut(s) 7, 10, 102
Bme1390I CCNGG 1 cut(s) 361
Bme18I GGWCC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 541
BmiI GGNNCC 3 cut(s) 465, 542, 543
BmrFI CCNGG 1 cut(s) 361
BpiI GAAGAC 4 cut(s) 21, 245, 263, 303
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 537, 550
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 92
BseGI GGATG 3 cut(s) 167, 357, 369
BseLI CCNNNNNNNGG 2 cut(s) 537, 550
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 596
BseSI GKGCMC 2 cut(s) 456, 468
BseXI GCAGC 2 cut(s) 17, 87
BshFI GGCC 3 cut(s) 84, 220, 595
BshNI GGYRCC 1 cut(s) 463
BslFI GGGAC 2 cut(s) 508, 554
BslI CCNNNNNNNGG 2 cut(s) 537, 550
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 2 cut(s) 508, 554
BsnI GGCC 3 cut(s) 84, 220, 595
Bso31I GGTCTC 1 cut(s) 443
Bsp1286I GDGCHC 2 cut(s) 456, 468
Bsp143I GATC 2 cut(s) 608, 623
BspACI CCGC 1 cut(s) 506
BspANI GGCC 3 cut(s) 84, 220, 595
BspCNI CTCAG 1 cut(s) 595
BspLI GGNNCC 3 cut(s) 465, 542, 543
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 463
BspTI CTTAAG 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 2 cut(s) 608, 623
BssT1I CCWWGG 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 3 cut(s) 278, 376, 390
Bst6I CTCTTC 1 cut(s) 297
BstAFI CTTAAG 1 cut(s) 86
BstDEI CTNAG 2 cut(s) 582, 614
BstF5I GGATG 3 cut(s) 167, 357, 369
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 611, 626
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 608, 623
BstMWI GCNNNNNNNGC 2 cut(s) 14, 97
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 359
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 2 cut(s) 456, 468
BstV1I GCAGC 2 cut(s) 17, 87
BstV2I GAAGAC 4 cut(s) 21, 245, 263, 303
BstX2I RGATCY 1 cut(s) 608
BstYI RGATCY 1 cut(s) 608
BsuRI GGCC 3 cut(s) 84, 220, 595
BtsCI GGATG 3 cut(s) 167, 357, 369
BtsIMutI CAGTG 2 cut(s) 283, 386
BveI ACCTGC 1 cut(s) 8
CfoI GCGC 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 541
CseI GACGC 1 cut(s) 106
CspCI CAANNNNNGTGG 2 cut(s) 259, 294
CviAII CATG 2 cut(s) 24, 459
DdeI CTNAG 2 cut(s) 582, 614
DpnI GATC 2 cut(s) 610, 625
DpnII GATC 2 cut(s) 608, 623
EaeI YGGCCR 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 92
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 541
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 359
EcoT14I CCWWGG 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 2 cut(s) 27, 462
FaiI YATR 6 cut(s) 25, 326, 395, 460, 519, 548
FaqI GGGAC 2 cut(s) 508, 554
FatI CATG 2 cut(s) 23, 458
FauI CCCGC 1 cut(s) 499
Fnu4HI GCNGC 3 cut(s) 6, 9, 101
FokI GGATG 3 cut(s) 154, 364, 376
Fsp4HI GCNGC 3 cut(s) 6, 9, 101
FspBI CTAG 2 cut(s) 534, 637
GlaI GCGC 1 cut(s) 104
GluI GCNGC 3 cut(s) 6, 9, 101
HaeIII GGCC 3 cut(s) 84, 220, 595
HgaI GACGC 1 cut(s) 106
HhaI GCGC 1 cut(s) 105
Hin1II CATG 2 cut(s) 27, 462
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 2 cut(s) 199, 369
Hpy188I TCNGA 4 cut(s) 131, 153, 215, 585
Hpy188III TCNNGA 1 cut(s) 196
HpyAV CCTTC 5 cut(s) 202, 305, 314, 478, 548
HpyCH4III ACNGT 3 cut(s) 278, 376, 390
HpyCH4V TGCA 2 cut(s) 11, 17
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 97
HpyF3I CTNAG 2 cut(s) 582, 614
Hsp92II CATG 2 cut(s) 27, 462
HspAI GCGC 1 cut(s) 103
KflI GGGWCCC 1 cut(s) 541
Kzo9I GATC 2 cut(s) 608, 623
LpnPI CCDG 6 cut(s) 3, 334, 345, 346, 373, 609
Lsp1109I GCAGC 2 cut(s) 17, 87
MaeI CTAG 2 cut(s) 534, 637
MaeIII GTNAC 1 cut(s) 340
MalI GATC 2 cut(s) 610, 625
MboI GATC 2 cut(s) 608, 623
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 2 cut(s) 456, 468
MluCI AATT 3 cut(s) 179, 444, 589
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 9 cut(s) 49, 181, 200, 286, 298, 541, 543, 571, 621
MseI TTAA 3 cut(s) 75, 87, 182
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 8
MspCI CTTAAG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 361
MvaI CCWGG 1 cut(s) 361
MwoI GCNNNNNNNGC 2 cut(s) 14, 97
NdeII GATC 2 cut(s) 608, 623
NlaIII CATG 2 cut(s) 27, 462
NlaIV GGNNCC 3 cut(s) 465, 542, 543
NmuCI GTSAC 1 cut(s) 340
OliI CACNNNNGTG 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PfeI GAWTC 2 cut(s) 199, 369
PkrI GCNGC 3 cut(s) 7, 10, 102
PpuMI RGGWCCY 1 cut(s) 541
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 465, 542, 543
PspPI GGNCC 1 cut(s) 541
PspPPI RGGWCCY 1 cut(s) 541
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 608
PvuII CAGCTG 1 cut(s) 8
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 3 cut(s) 75, 87, 182
SatI GCNGC 3 cut(s) 6, 9, 101
Sau3AI GATC 2 cut(s) 608, 623
Sau96I GGNCC 1 cut(s) 541
ScrFI CCNGG 1 cut(s) 361
SduI GDGCHC 2 cut(s) 456, 468
SetI ASST 5 cut(s) 10, 22, 194, 502, 518
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 3 cut(s) 179, 444, 589
SsiI CCGC 1 cut(s) 506
SspMI CTAG 2 cut(s) 534, 637
StyD4I CCNGG 1 cut(s) 359
StyI CCWWGG 1 cut(s) 92
TaaI ACNGT 3 cut(s) 278, 376, 390
TaqI TCGA 1 cut(s) 626
TasI AATT 3 cut(s) 179, 444, 589
TfiI GAWTC 2 cut(s) 199, 369
Tru1I TTAA 3 cut(s) 75, 87, 182
Tru9I TTAA 3 cut(s) 75, 87, 182
TscAI CASTG 2 cut(s) 283, 393
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 3 cut(s) 5, 8, 100
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 4 cut(s) 12, 153, 300, 460
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 2 cut(s) 283, 393
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 541
XspI CTAG 2 cut(s) 534, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.