FvH4_5g27181

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014331
Physical Location & Seq
Reverse (-)
18449278 .. 18452407
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27181.t1

Sequence Viewer

Length: 996 bp
ATGGCTTCATCATCGGAAAAGGCCGTGTGCGTCACCGGCGGCAATGGCTTCATCGGCTCCTGGCTAGTCAAGACCCTCTTAGAACGCGGCTACACTCCCGTCCACGTCTCCATCTACCCCGGCTCCGACGCCTCCCACCTCTTCGCCCTCCCCGGCGCCTCCCCCGCCACCCTCACCGTCTTCGAGGCCGACATCCTTGACGCCGACGCCGTCTCCAGAGCCGTCCAGAATTGCAAGGGCGTCTTCCACGTGGCCTCCCCCTGCACCCTCGACGACCCGAAAGACCCGCAGGCCGAGCTTGTCCTCCCCGCCGTCCAGGGCACCCTCAATGTCCTCCAGGCCGCTTCCAGCTTCGGCGTCCGGCGCGTCGTCCTCACCTCCTCCATCTCTTCCATGGTCCCCAACCCCGGTTGGCCCAGCCACAAAGTCTTCGACGAGACGTCTTGGACCGATCTCGATTACTGCAAATCCCGCCAGAAATGGTATCCGGTGTCGAAGACACTAGCAGAGAAGGCGGCTTGGGATTATGCAGAGAAGAATGGATTAGATGTGGTAGCAATCCATCCAGCCACATGTCTCGGACCTCTTCTGCAGCCTGGTTTGAATGCCAGCTGTGCTGTTCTGCTCAACTTGCTGCAGGGATCCACAGACACACAAGAGTACCACTGGTTGGGGGCTGTGCATGTCAGAGATGTCGCCAAGGCGCAAGTTTTGCTGTTTGAGACTCCTGCGGCTTCTGGGAGGTACCTTTGCACCAATGGTATATATCAATTCGGTGACTTTGCAGATAGAGTCTCCAAACTCTTCCCCCAGTTTCCGGTTCACAGGTTTAACGGAGAAACCCAACCAGGCCTCAAGGATTGCAAGGATGCTGCAAAGAGATTAATCGACCTGGGTCTGGTTTTCACACCAATTGAAGATGCTGTCCGGGACACAGTGGAGAGCCTCAAAGCTAGAGGCTTTTTGAAGCAGGAAGAGACGTCACAATCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

332

Amino Acids

35.78

Weight (kDa)

5.76

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 9 - 246 3.3e-27 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 128 2e-07 Polysaccharide biosynthesis protein
KR PF08659 10 - 137 5.3e-08 KR domain
3Beta_HSD PF01073 11 - 238 4.9e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 264 1e-14 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 190 9.7e-12 NAD(P)H-binding
NAD_binding_4 PF07993 66 - 194 1.9e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02400
fragaria_vesca FvH4_5g27181
malus_domestica MD08G1250300.v1.1
prunus_persica Prupe.1G587500_v2.0.a1 Prupe.1G587500_v2.0.a1
pyrus_communis pycom08g21710
rosa_chinensis RchiOBHm_Chr7g0218281
rosa_laevigata RLG00000002382
rosa_multiflora Rmu_co8255051.1_g000001 Rmu_sc0002408.1_g000014 Rmu_sc0004956.1_g000010
rosa_roxburghii Rroxscaffold_3G00241130
rosa_rugosa Rorug07G0176100.1
rosa_samantha Rh7AG317800 Rh7BG309100 Rh7DG316700
rosa_wichuraiana Rw7G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 443, 983
Acc65I GGTACC 1 cut(s) 744
AccB1I GGYRCC 3 cut(s) 155, 320, 744
AccB7I CCANNNNNTGG 1 cut(s) 670
AccII CGCG 2 cut(s) 87, 366
AciI CCGC 9 cut(s) 39, 87, 165, 287, 309, 342, 472, 515, 731
AclWI GGATC 2 cut(s) 636, 649
AcvI CACGTG 1 cut(s) 250
AcyI GRCGYC 8 cut(s) 129, 156, 201, 207, 240, 357, 440, 980
AfaI GTAC 2 cut(s) 662, 746
AfiI CCNNNNNNNGG 4 cut(s) 407, 670, 817, 898
AflIII ACRYGT 1 cut(s) 572
AgsI TTSAA 3 cut(s) 604, 917, 967
AhdI GACNNNNNGTC 1 cut(s) 439
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 6 cut(s) 59, 315, 336, 595, 847, 891
AluBI AGCT 5 cut(s) 298, 351, 612, 953, 993
AluI AGCT 5 cut(s) 298, 351, 612, 953, 993
Alw26I GTCTC 7 cut(s) 112, 217, 431, 581, 716, 799, 971
AlwI GGATC 2 cut(s) 636, 649
AoxI GGCC 7 cut(s) 21, 186, 252, 291, 339, 413, 850
ApeKI GCWGC 3 cut(s) 592, 634, 872
AseI ATTAAT 1 cut(s) 884
Asp718I GGTACC 1 cut(s) 744
AspLEI GCGC 3 cut(s) 158, 366, 706
AspS9I GGNCC 4 cut(s) 397, 414, 447, 581
AsuC2I CCSGG 4 cut(s) 120, 153, 408, 929
AsuHPI GGTGA 4 cut(s) 25, 166, 367, 788
AvaII GGWCC 3 cut(s) 397, 447, 581
BaeGI GKGCMC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 644, 677
BamHI GGATCC 1 cut(s) 641
BanI GGYRCC 3 cut(s) 155, 320, 744
BbrPI CACGTG 1 cut(s) 250
BbsI GAAGAC 4 cut(s) 172, 235, 421, 503
BbvI GCAGC 3 cut(s) 604, 621, 859
BccI CCATC 3 cut(s) 119, 392, 570
BceAI ACGGC 4 cut(s) 8, 194, 206, 296
BciT130I CCWGG 6 cut(s) 61, 317, 338, 597, 849, 893
BciVI GTATCC 1 cut(s) 495
BcnI CCSGG 4 cut(s) 120, 153, 408, 929
BcoDI GTCTC 7 cut(s) 112, 217, 431, 581, 716, 799, 971
BfaI CTAG 5 cut(s) 65, 503, 954, 990, 994
BfmI CTRYAG 2 cut(s) 590, 635
BfoI RGCGCY 1 cut(s) 159
BfuI GTATCC 1 cut(s) 495
BisI GCNGC 8 cut(s) 40, 88, 342, 516, 593, 635, 732, 873
BlsI GCNGC 8 cut(s) 41, 89, 343, 517, 594, 636, 733, 874
Bme18I GGWCC 3 cut(s) 397, 447, 581
BmeRI GACNNNNNGTC 1 cut(s) 439
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 397, 414, 447, 581
BmiI GGNNCC 7 cut(s) 58, 124, 157, 322, 399, 643, 746
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 2 cut(s) 859, 910
BmuI ACTGGG 1 cut(s) 805
BoxI GACNNNNGTC 1 cut(s) 894
BpiI GAAGAC 4 cut(s) 172, 235, 421, 503
BpmI CTGGAG 2 cut(s) 199, 320
BpuEI CTTGAG 1 cut(s) 839
BpuMI CCSGG 4 cut(s) 120, 153, 408, 929
BsaAI YACGTR 1 cut(s) 250
BsaHI GRCGYC 8 cut(s) 129, 156, 201, 207, 240, 357, 440, 980
BsaJI CCNNGG 7 cut(s) 118, 151, 316, 393, 406, 699, 892
BsaWI WCCGGW 2 cut(s) 487, 817
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 197, 227, 239, 269
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 670, 817, 898
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 2 cut(s) 671, 811
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 6 cut(s) 61, 317, 338, 597, 849, 893
BseDI CCNNGG 7 cut(s) 118, 151, 316, 393, 406, 699, 892
BseGI GGATG 3 cut(s) 192, 562, 874
BseLI CCNNNNNNNGG 4 cut(s) 407, 670, 817, 898
BseMI GCAATG 1 cut(s) 49
BseNI ACTGG 2 cut(s) 671, 811
BseRI GAGGAG 1 cut(s) 370
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 3 cut(s) 604, 621, 859
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 247
Bsh1236I CGCG 2 cut(s) 87, 366
BshFI GGCC 7 cut(s) 23, 188, 254, 293, 341, 415, 852
BshNI GGYRCC 3 cut(s) 155, 320, 744
BsiSI CCGG 8 cut(s) 36, 120, 153, 361, 408, 488, 818, 928
BslFI GGGAC 2 cut(s) 383, 944
BslI CCNNNNNNNGG 4 cut(s) 407, 670, 817, 898
BsmAI GTCTC 7 cut(s) 112, 217, 431, 581, 716, 799, 971
BsmBI CGTCTC 4 cut(s) 112, 217, 431, 971
BsmFI GGGAC 2 cut(s) 383, 944
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 7 cut(s) 23, 188, 254, 293, 341, 415, 852
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 2 cut(s) 451, 641
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 9 cut(s) 39, 87, 165, 287, 309, 342, 472, 515, 731
BspANI GGCC 7 cut(s) 23, 188, 254, 293, 341, 415, 852
BspFNI CGCG 2 cut(s) 87, 366
BspLI GGNNCC 7 cut(s) 58, 124, 157, 322, 399, 643, 746
BspMAI CTGCAG 2 cut(s) 594, 639
BspPI GGATC 2 cut(s) 636, 649
BspT107I GGYRCC 3 cut(s) 155, 320, 744
BsrDI GCAATG 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 2 cut(s) 671, 811
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 7 cut(s) 118, 151, 316, 393, 406, 699, 892
BssMI GATC 2 cut(s) 451, 641
BssNI GRCGYC 8 cut(s) 129, 156, 201, 207, 240, 357, 440, 980
BssT1I CCWWGG 2 cut(s) 393, 699
Bst2UI CCWGG 6 cut(s) 61, 317, 338, 597, 849, 893
Bst4CI ACNGT 2 cut(s) 178, 937
Bst6I CTCTTC 5 cut(s) 146, 394, 591, 809, 969
BstACI GRCGYC 8 cut(s) 129, 156, 201, 207, 240, 357, 440, 980
BstAPI GCANNNNNTGC 1 cut(s) 712
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 2 cut(s) 291, 610
BstDEI CTNAG 1 cut(s) 79
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 3 cut(s) 192, 562, 874
BstFNI CGCG 2 cut(s) 87, 366
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 3 cut(s) 158, 366, 706
BstKTI GATC 2 cut(s) 454, 644
BstMAI GTCTC 7 cut(s) 112, 217, 431, 581, 716, 799, 971
BstMBI GATC 2 cut(s) 451, 641
BstNI CCWGG 6 cut(s) 61, 317, 338, 597, 849, 893
BstNSI RCATGY 2 cut(s) 576, 686
BstPAI GACNNNNGTC 1 cut(s) 894
BstSFI CTRYAG 2 cut(s) 590, 635
BstSLI GKGCMC 1 cut(s) 323
BstUI CGCG 2 cut(s) 87, 366
BstV1I GCAGC 3 cut(s) 604, 621, 859
BstV2I GAAGAC 4 cut(s) 172, 235, 421, 503
BstX2I RGATCY 1 cut(s) 641
BstYI RGATCY 1 cut(s) 641
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 7 cut(s) 23, 188, 254, 293, 341, 415, 852
BtgI CCRYGG 1 cut(s) 393
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 3 cut(s) 192, 562, 874
BtsIMutI CAGTG 2 cut(s) 664, 942
Cac8I GCNNGC 2 cut(s) 291, 610
CfoI GCGC 3 cut(s) 158, 366, 706
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 4 cut(s) 397, 414, 447, 581
CseI GACGC 7 cut(s) 19, 137, 209, 215, 229, 346, 355
Csp6I GTAC 2 cut(s) 661, 745
CviAII CATG 3 cut(s) 394, 573, 683
CviQI GTAC 2 cut(s) 661, 745
DdeI CTNAG 1 cut(s) 79
DinI GGCGCC 1 cut(s) 157
DpnI GATC 2 cut(s) 453, 643
DpnII GATC 2 cut(s) 451, 641
DriI GACNNNNNGTC 1 cut(s) 439
Eam1104I CTCTTC 5 cut(s) 146, 394, 591, 809, 969
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 5 cut(s) 146, 394, 591, 809, 969
Eco130I CCWWGG 2 cut(s) 393, 699
Eco147I AGGCCT 1 cut(s) 852
Eco47I GGWCC 3 cut(s) 397, 447, 581
Eco72I CACGTG 1 cut(s) 250
EcoRII CCWGG 6 cut(s) 59, 315, 336, 595, 847, 891
EcoT14I CCWWGG 2 cut(s) 393, 699
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 393, 699
Esp3I CGTCTC 4 cut(s) 112, 217, 431, 971
FaeI CATG 3 cut(s) 397, 576, 686
FaiI YATR 6 cut(s) 395, 528, 574, 684, 764, 766
FalI AAGNNNNNCTT 4 cut(s) 62, 94, 227, 259
FaqI GGGAC 2 cut(s) 383, 944
FatI CATG 3 cut(s) 393, 572, 682
FauI CCCGC 4 cut(s) 172, 294, 316, 479
Fnu4HI GCNGC 8 cut(s) 40, 88, 342, 516, 593, 635, 732, 873
FokI GGATG 3 cut(s) 179, 549, 881
Fsp4HI GCNGC 8 cut(s) 40, 88, 342, 516, 593, 635, 732, 873
FspBI CTAG 5 cut(s) 65, 503, 954, 990, 994
GlaI GCGC 3 cut(s) 157, 365, 705
GluI GCNGC 8 cut(s) 40, 88, 342, 516, 593, 635, 732, 873
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 2 cut(s) 199, 320
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 7 cut(s) 23, 188, 254, 293, 341, 415, 852
HapII CCGG 8 cut(s) 36, 120, 153, 361, 408, 488, 818, 928
HgaI GACGC 7 cut(s) 19, 137, 209, 215, 229, 346, 355
HhaI GCGC 3 cut(s) 158, 366, 706
Hin1I GRCGYC 8 cut(s) 129, 156, 201, 207, 240, 357, 440, 980
Hin1II CATG 3 cut(s) 397, 576, 686
Hin6I GCGC 3 cut(s) 156, 364, 704
HinP1I GCGC 3 cut(s) 156, 364, 704
HinfI GANTC 2 cut(s) 724, 792
HpaII CCGG 8 cut(s) 36, 120, 153, 361, 408, 488, 818, 928
HphI GGTGA 4 cut(s) 25, 166, 367, 788
Hpy166II GTNNAC 2 cut(s) 103, 823
Hpy188I TCNGA 4 cut(s) 16, 127, 581, 689
Hpy188III TCNNGA 4 cut(s) 70, 216, 226, 455
Hpy8I GTNNAC 2 cut(s) 103, 823
Hpy99I CGWCG 5 cut(s) 131, 209, 275, 371, 437
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 2 cut(s) 178, 937
HpyCH4IV ACGT 4 cut(s) 105, 249, 440, 980
HpyF3I CTNAG 1 cut(s) 79
HpySE526I ACGT 4 cut(s) 105, 249, 440, 980
Hsp92I GRCGYC 8 cut(s) 129, 156, 201, 207, 240, 357, 440, 980
Hsp92II CATG 3 cut(s) 397, 576, 686
HspAI GCGC 3 cut(s) 156, 364, 704
KasI GGCGCC 1 cut(s) 155
KpnI GGTACC 1 cut(s) 748
Kzo9I GATC 2 cut(s) 451, 641
LmnI GCTCC 2 cut(s) 62, 128
Lsp1109I GCAGC 3 cut(s) 604, 621, 859
LweI GCATC 2 cut(s) 859, 910
MaeI CTAG 5 cut(s) 65, 503, 954, 990, 994
MaeII ACGT 4 cut(s) 105, 249, 440, 980
MaeIII GTNAC 3 cut(s) 31, 776, 981
MalI GATC 2 cut(s) 453, 643
MboI GATC 2 cut(s) 451, 641
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 1 cut(s) 641
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 3 cut(s) 229, 770, 912
Mly113I GGCGCC 1 cut(s) 156
MlyI GAGTC 2 cut(s) 718, 801
MmeI TCCRAC 1 cut(s) 150
MseI TTAA 2 cut(s) 831, 884
MspA1I CMGCKG 1 cut(s) 612
MspI CCGG 8 cut(s) 36, 120, 153, 361, 408, 488, 818, 928
MunI CAATTG 1 cut(s) 912
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 6 cut(s) 61, 317, 338, 597, 849, 893
MvnI CGCG 2 cut(s) 87, 366
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 4 cut(s) 120, 153, 408, 929
NcoI CCATGG 1 cut(s) 393
NdeII GATC 2 cut(s) 451, 641
NlaIII CATG 3 cut(s) 397, 576, 686
NlaIV GGNNCC 7 cut(s) 58, 124, 157, 322, 399, 643, 746
NmeAIII GCCGAG 1 cut(s) 319
NmuCI GTSAC 3 cut(s) 31, 776, 981
NspI RCATGY 2 cut(s) 576, 686
PceI AGGCCT 1 cut(s) 852
PciI ACATGT 1 cut(s) 572
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 610
PflFI GACNNNGTC 1 cut(s) 209
PflMI CCANNNNNTGG 1 cut(s) 670
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 8 cut(s) 41, 89, 343, 517, 594, 636, 733, 874
PleI GAGTC 2 cut(s) 718, 800
PluTI GGCGCC 1 cut(s) 159
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 2 cut(s) 718, 800
Ppu21I YACGTR 1 cut(s) 250
PscI ACATGT 1 cut(s) 572
PshAI GACNNNNGTC 1 cut(s) 894
PshBI ATTAAT 1 cut(s) 884
Psp6I CCWGG 6 cut(s) 59, 315, 336, 595, 847, 891
PspCI CACGTG 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 6 cut(s) 59, 315, 336, 595, 847, 891
PspN4I GGNNCC 7 cut(s) 58, 124, 157, 322, 399, 643, 746
PspPI GGNCC 4 cut(s) 397, 414, 447, 581
PstI CTGCAG 2 cut(s) 594, 639
PsuI RGATCY 1 cut(s) 641
PsyI GACNNNGTC 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 2 cut(s) 662, 746
RsaNI GTAC 2 cut(s) 661, 745
SaqAI TTAA 2 cut(s) 831, 884
SatI GCNGC 8 cut(s) 40, 88, 342, 516, 593, 635, 732, 873
Sau3AI GATC 2 cut(s) 451, 641
Sau96I GGNCC 4 cut(s) 397, 414, 447, 581
SchI GAGTC 2 cut(s) 718, 801
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 2 cut(s) 859, 910
SfcI CTRYAG 2 cut(s) 590, 635
SfoI GGCGCC 1 cut(s) 157
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 3 cut(s) 397, 447, 581
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 3 cut(s) 229, 770, 912
SseBI AGGCCT 1 cut(s) 852
SsiI CCGC 9 cut(s) 39, 87, 165, 287, 309, 342, 472, 515, 731
SspDI GGCGCC 1 cut(s) 155
SspMI CTAG 5 cut(s) 65, 503, 954, 990, 994
StuI AGGCCT 1 cut(s) 852
StyI CCWWGG 2 cut(s) 393, 699
TaaI ACNGT 2 cut(s) 178, 937
TaiI ACGT 4 cut(s) 108, 252, 443, 983
TaqI TCGA 6 cut(s) 183, 270, 432, 456, 494, 888
TaqII GACCGA 1 cut(s) 464
TasI AATT 3 cut(s) 229, 770, 912
TauI GCSGC 5 cut(s) 42, 90, 344, 518, 734
Tru1I TTAA 2 cut(s) 831, 884
Tru9I TTAA 2 cut(s) 831, 884
TscAI CASTG 2 cut(s) 671, 942
TseFI GTSAC 3 cut(s) 31, 776, 981
TseI GCWGC 3 cut(s) 592, 634, 872
Tsp45I GTSAC 3 cut(s) 31, 776, 981
TspDTI ATGAA 1 cut(s) 40
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 2 cut(s) 671, 942
Tth111I GACNNNGTC 1 cut(s) 209
Van91I CCANNNNNTGG 1 cut(s) 670
VpaK11BI GGWCC 3 cut(s) 397, 447, 581
VspI ATTAAT 1 cut(s) 884
XceI RCATGY 2 cut(s) 576, 686
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XspI CTAG 5 cut(s) 65, 503, 954, 990, 994
ZraI GACGTC 2 cut(s) 441, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.