MD08G1250300.v1.1

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014331
Physical Location & Seq
Forward (+)
31513871 .. 31516401
2531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1250300.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGGATGAAACGAAAAACGAGGAGGCGGTGTGCGTGACTGGAGCCAATGGCTTCATAGGGTCGTGGTTGGTCAAGACATTACTGGAAGAGGGCTACACCCGCATCCACGCCTCCATCTTCCCTGCCTCCGATCCCTCCCACCTCTTCTCCCTCCCCGGCGCCGACCGCCACACTATCACGGTCTTTGAGGCCGACCTCCTCGACGCCGACGCAGTCGCCAGGGCCGTCCAGCACTGCCAGGGTGTCTTCCACGTGGCTTCCCCCTGCACCCTCGACGACCCCACCGACCCCCAGGCTGAGCTGGTGTTGCCTGCCGTGCAGGGCACCCTCAATGTGCTCCAAGCCGCCTCGAAGTTCGGAGTGAGGCGCGTCGTCCTCACCTCCTCAATCTCTGCCATGGTCCCCAACCCTTCTTGGCCTCCTCACAAGCCCTTCGACGAGTCCTCTTGGACCGACCTCGACTACTGCAAAGCCCGCCAGAAATGGTATCCGGTGTCGAAGACAGAGGCAGAGAAAGCAGCTTGGGAGTTTGCAGAGAAGCATGGACTGGATGTGGTAGCCATCCATCCGGCCACATGTCTTGGGCCTCTCCAGCAGCCTAGCCTCAATGCAAGCTCTGCTGTTTTGCTCAACTTGCTCCTTGGATCCAACGATACCCAAGAGCATCACTGGTTGGGGGCTGTCCATGTCCAAGATGTCGTCAAGGCACAAGTTTTGCTCTTTGAATCTCGTGCTGCTTCTGGCAGGTACCTCTGCACCAATGGCATCTTCCAATTCGCTAACTTTGCCCTCACTGTCTCCAAACTCTTCCCTCACTTTCCCATTCACAGGTTTAGCGGGGAAACCCAGCCAGGGCTAAAGGAATGCAAGGATGCTGCAAAGCGATTAATTGAGCTGGGGTTGGTGTTCAAACCAGTTGAAGATGCTGTGCAGGACACAGTGGAGAGCCTCAAAGCGAAAGGCTTTTTGAAACCGGAAATATCACCATCAAACTCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

334

Amino Acids

36.34

Weight (kDa)

5.79

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 10 - 245 1.9e-27 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 10 - 135 1.5e-07 Polysaccharide biosynthesis protein
KR PF08659 11 - 137 8.5e-07 KR domain
3Beta_HSD PF01073 12 - 239 2.8e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 12 - 260 2.6e-15 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 14 - 191 1.3e-13 NAD(P)H-binding
NAD_binding_4 PF07993 67 - 195 3.2e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02400
fragaria_vesca FvH4_5g27181
malus_domestica MD08G1250300.v1.1
prunus_persica Prupe.1G587500_v2.0.a1 Prupe.1G587500_v2.0.a1
pyrus_communis pycom08g21710
rosa_chinensis RchiOBHm_Chr7g0218281
rosa_laevigata RLG00000002382
rosa_multiflora Rmu_co8255051.1_g000001 Rmu_sc0002408.1_g000014 Rmu_sc0004956.1_g000010
rosa_roxburghii Rroxscaffold_3G00241130
rosa_rugosa Rorug07G0176100.1
rosa_samantha Rh7AG317800 Rh7BG309100 Rh7DG316700
rosa_wichuraiana Rw7G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 735
Acc65I GGTACC 1 cut(s) 747
AccB1I GGYRCC 3 cut(s) 158, 323, 747
AccII CGCG 1 cut(s) 369
AciI CCGC 6 cut(s) 26, 100, 166, 345, 475, 837
AclWI GGATC 3 cut(s) 125, 639, 652
AcoI YGGCCR 1 cut(s) 570
AcvI CACGTG 1 cut(s) 253
AcyI GRCGYC 2 cut(s) 159, 204
AfaI GTAC 1 cut(s) 749
AfiI CCNNNNNNNGG 2 cut(s) 828, 852
AflIII ACRYGT 1 cut(s) 575
AgsI TTSAA 4 cut(s) 725, 910, 920, 970
AjnI CCWGG 4 cut(s) 218, 237, 291, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 752, 784
AluBI AGCT 4 cut(s) 301, 521, 615, 895
AluI AGCT 4 cut(s) 301, 521, 615, 895
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 802
AlwI GGATC 3 cut(s) 125, 639, 652
AoxI GGCC 5 cut(s) 189, 222, 416, 570, 584
ApeKI GCWGC 4 cut(s) 518, 595, 734, 875
AseI ATTAAT 1 cut(s) 887
Asp718I GGTACC 1 cut(s) 747
AspLEI GCGC 2 cut(s) 161, 369
AspS9I GGNCC 4 cut(s) 222, 400, 450, 584
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 370, 975
AvaII GGWCC 2 cut(s) 400, 450
BaeGI GKGCMC 1 cut(s) 326
BamHI GGATCC 1 cut(s) 644
BanI GGYRCC 3 cut(s) 158, 323, 747
BauI CACGAG 1 cut(s) 729
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 238, 506
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 4 cut(s) 530, 607, 721, 862
BccI CCATC 4 cut(s) 122, 569, 573, 994
BceAI ACGGC 2 cut(s) 209, 299
BciT130I CCWGG 4 cut(s) 220, 239, 293, 852
BciVI GTATCC 1 cut(s) 498
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 802
BfaI CTAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 735
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 5 cut(s) 345, 519, 596, 735, 876
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 5 cut(s) 346, 520, 597, 736, 877
Bme1390I CCNGG 5 cut(s) 156, 220, 239, 293, 852
Bme18I GGWCC 2 cut(s) 400, 450
BmgT120I GGNCC 4 cut(s) 222, 400, 450, 584
BmiI GGNNCC 6 cut(s) 43, 160, 325, 402, 646, 749
BmrFI CCNGG 5 cut(s) 156, 220, 239, 293, 852
BmsI GCATC 5 cut(s) 111, 673, 774, 862, 913
BpiI GAAGAC 2 cut(s) 238, 506
BpmI CTGGAG 2 cut(s) 60, 575
Bpu1102I GCTNAGC 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 2 cut(s) 159, 204
BsaJI CCNNGG 7 cut(s) 154, 219, 238, 291, 396, 640, 851
BsaWI WCCGGW 2 cut(s) 490, 973
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 828, 852
Bse1I ACTGG 5 cut(s) 43, 87, 552, 674, 914
BseBI CCWGG 4 cut(s) 220, 239, 293, 852
BseDI CCNNGG 7 cut(s) 154, 219, 238, 291, 396, 640, 851
BseGI GGATG 6 cut(s) 10, 102, 556, 561, 565, 877
BseLI CCNNNNNNNGG 2 cut(s) 828, 852
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 5 cut(s) 43, 87, 552, 674, 914
BseRI GAGGAG 4 cut(s) 35, 188, 373, 411
BseSI GKGCMC 1 cut(s) 326
BseXI GCAGC 4 cut(s) 530, 607, 721, 862
BseYI CCCAGC 2 cut(s) 846, 895
BsgI GTGCAG 4 cut(s) 250, 338, 739, 950
Bsh1236I CGCG 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 191, 224, 418, 572, 586
BshNI GGYRCC 3 cut(s) 158, 323, 747
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 4 cut(s) 156, 491, 569, 974
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 828, 852
BsmAI GTCTC 1 cut(s) 802
BsmFI GGGAC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 869
BsnI GGCC 5 cut(s) 191, 224, 418, 572, 586
Bsp1286I GDGCHC 2 cut(s) 326, 339
Bsp143I GATC 2 cut(s) 130, 644
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 6 cut(s) 26, 100, 166, 345, 475, 837
BspANI GGCC 5 cut(s) 191, 224, 418, 572, 586
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 6 cut(s) 43, 160, 325, 402, 646, 749
BspMI ACCTGC 1 cut(s) 735
BspPI GGATC 3 cut(s) 125, 639, 652
BspT107I GGYRCC 3 cut(s) 158, 323, 747
BsrI ACTGG 5 cut(s) 43, 87, 552, 674, 914
BssECI CCNNGG 7 cut(s) 154, 219, 238, 291, 396, 640, 851
BssMI GATC 2 cut(s) 130, 644
BssNI GRCGYC 2 cut(s) 159, 204
BssSI CACGAG 1 cut(s) 729
BssT1I CCWWGG 2 cut(s) 396, 640
Bst2BI CACGAG 1 cut(s) 729
Bst2UI CCWGG 4 cut(s) 220, 239, 293, 852
Bst4CI ACNGT 3 cut(s) 181, 796, 940
Bst6I CTCTTC 3 cut(s) 81, 149, 812
BstACI GRCGYC 2 cut(s) 159, 204
BstAPI GCANNNNNTGC 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 312, 475, 613
BstDEI CTNAG 1 cut(s) 297
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 6 cut(s) 10, 102, 556, 561, 565, 877
BstFNI CGCG 1 cut(s) 369
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 161, 369
BstKTI GATC 2 cut(s) 133, 647
BstMAI GTCTC 1 cut(s) 802
BstMBI GATC 2 cut(s) 130, 644
BstMCI CGRYCG 1 cut(s) 166
BstNI CCWGG 4 cut(s) 220, 239, 293, 852
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 5 cut(s) 154, 218, 237, 291, 850
BstSLI GKGCMC 1 cut(s) 326
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 4 cut(s) 530, 607, 721, 862
BstV2I GAAGAC 2 cut(s) 238, 506
BstX2I RGATCY 1 cut(s) 644
BstYI RGATCY 1 cut(s) 644
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 5 cut(s) 191, 224, 418, 572, 586
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 6 cut(s) 10, 102, 556, 561, 565, 877
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 4 cut(s) 232, 667, 792, 945
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 312, 475, 613
CfoI GCGC 2 cut(s) 161, 369
Cfr13I GGNCC 4 cut(s) 222, 400, 450, 584
CseI GACGC 3 cut(s) 212, 218, 358
Csp6I GTAC 1 cut(s) 748
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 4 cut(s) 397, 542, 576, 686
CviQI GTAC 1 cut(s) 748
DdeI CTNAG 1 cut(s) 297
DinI GGCGCC 1 cut(s) 160
DpnI GATC 2 cut(s) 132, 646
DpnII GATC 2 cut(s) 130, 644
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 3 cut(s) 81, 149, 812
EarI CTCTTC 3 cut(s) 81, 149, 812
Eco130I CCWWGG 2 cut(s) 396, 640
Eco47I GGWCC 2 cut(s) 400, 450
Eco72I CACGTG 1 cut(s) 253
EcoRII CCWGG 4 cut(s) 218, 237, 291, 850
EcoT14I CCWWGG 2 cut(s) 396, 640
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 396, 640
FaeI CATG 4 cut(s) 400, 545, 579, 689
FaiI YATR 5 cut(s) 56, 398, 543, 577, 687
FaqI GGGAC 1 cut(s) 386
FatI CATG 4 cut(s) 396, 541, 575, 685
FauI CCCGC 3 cut(s) 107, 482, 830
Fnu4HI GCNGC 5 cut(s) 345, 519, 596, 735, 876
FokI GGATG 6 cut(s) 17, 89, 548, 552, 563, 884
Fsp4HI GCNGC 5 cut(s) 345, 519, 596, 735, 876
FspBI CTAG 1 cut(s) 600
GlaI GCGC 2 cut(s) 160, 368
GluI GCNGC 5 cut(s) 345, 519, 596, 735, 876
GsaI CCCAGC 2 cut(s) 850, 899
GsuI CTGGAG 2 cut(s) 60, 575
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 5 cut(s) 191, 224, 418, 572, 586
HapII CCGG 4 cut(s) 156, 491, 569, 974
HgaI GACGC 3 cut(s) 212, 218, 358
HhaI GCGC 2 cut(s) 161, 369
Hin1I GRCGYC 2 cut(s) 159, 204
Hin1II CATG 4 cut(s) 400, 545, 579, 689
Hin6I GCGC 2 cut(s) 159, 367
HinP1I GCGC 2 cut(s) 159, 367
HinfI GANTC 2 cut(s) 440, 725
HpaII CCGG 4 cut(s) 156, 491, 569, 974
HphI GGTGA 2 cut(s) 370, 975
Hpy188I TCNGA 2 cut(s) 130, 359
Hpy188III TCNNGA 1 cut(s) 73
Hpy99I CGWCG 5 cut(s) 206, 212, 278, 374, 440
HpyAV CCTTC 2 cut(s) 420, 442
HpyCH4III ACNGT 3 cut(s) 181, 796, 940
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 9 cut(s) 267, 319, 468, 533, 611, 756, 867, 878, 931
HpyF3I CTNAG 1 cut(s) 297
HpySE526I ACGT 1 cut(s) 252
Hsp92I GRCGYC 2 cut(s) 159, 204
Hsp92II CATG 4 cut(s) 400, 545, 579, 689
HspAI GCGC 2 cut(s) 159, 367
KasI GGCGCC 1 cut(s) 158
KpnI GGTACC 1 cut(s) 751
Kzo9I GATC 2 cut(s) 130, 644
LmnI GCTCC 3 cut(s) 41, 342, 642
Lsp1109I GCAGC 4 cut(s) 530, 607, 721, 862
LweI GCATC 5 cut(s) 111, 673, 774, 862, 913
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 132, 646
MboI GATC 2 cut(s) 130, 644
MboII GAAGA 8 cut(s) 98, 109, 136, 238, 511, 760, 799, 932
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 2 cut(s) 326, 339
MluCI AATT 2 cut(s) 773, 888
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 672
MseI TTAA 1 cut(s) 887
MspI CCGG 4 cut(s) 156, 491, 569, 974
MspR9I CCNGG 5 cut(s) 156, 220, 239, 293, 852
Mva1269I GAATGC 1 cut(s) 869
MvaI CCWGG 4 cut(s) 220, 239, 293, 852
MvnI CGCG 1 cut(s) 369
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 396
NdeII GATC 2 cut(s) 130, 644
NlaIII CATG 4 cut(s) 400, 545, 579, 689
NlaIV GGNNCC 6 cut(s) 43, 160, 325, 402, 646, 749
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 579
PciI ACATGT 1 cut(s) 575
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 282
PctI GAATGC 1 cut(s) 869
PfeI GAWTC 1 cut(s) 725
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 5 cut(s) 346, 520, 597, 736, 877
PleI GAGTC 1 cut(s) 448
PluTI GGCGCC 1 cut(s) 162
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 1 cut(s) 448
Ppu21I YACGTR 1 cut(s) 253
PscI ACATGT 1 cut(s) 575
PshBI ATTAAT 1 cut(s) 887
Psp6I CCWGG 4 cut(s) 218, 237, 291, 850
PspCI CACGTG 1 cut(s) 253
PspFI CCCAGC 2 cut(s) 846, 895
PspGI CCWGG 4 cut(s) 218, 237, 291, 850
PspN4I GGNNCC 6 cut(s) 43, 160, 325, 402, 646, 749
PspPI GGNCC 4 cut(s) 222, 400, 450, 584
PsuI RGATCY 1 cut(s) 644
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 749
RsaNI GTAC 1 cut(s) 748
SaqAI TTAA 1 cut(s) 887
SatI GCNGC 5 cut(s) 345, 519, 596, 735, 876
Sau3AI GATC 2 cut(s) 130, 644
Sau96I GGNCC 4 cut(s) 222, 400, 450, 584
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 5 cut(s) 156, 220, 239, 293, 852
SduI GDGCHC 2 cut(s) 326, 339
SfaNI GCATC 5 cut(s) 111, 673, 774, 862, 913
SfoI GGCGCC 1 cut(s) 160
SinI GGWCC 2 cut(s) 400, 450
Sse9I AATT 2 cut(s) 773, 888
SsiI CCGC 6 cut(s) 26, 100, 166, 345, 475, 837
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 5 cut(s) 154, 218, 237, 291, 850
StyI CCWWGG 2 cut(s) 396, 640
TaaI ACNGT 3 cut(s) 181, 796, 940
TaiI ACGT 1 cut(s) 255
TaqI TCGA 6 cut(s) 201, 273, 350, 435, 459, 497
TaqII GACCGA 1 cut(s) 467
TasI AATT 2 cut(s) 773, 888
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 1 cut(s) 725
Tru1I TTAA 1 cut(s) 887
Tru9I TTAA 1 cut(s) 887
TscAI CASTG 4 cut(s) 239, 674, 799, 945
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 4 cut(s) 518, 595, 734, 875
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 21, 43
TspRI CASTG 4 cut(s) 239, 674, 799, 945
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 2 cut(s) 400, 450
VspI ATTAAT 1 cut(s) 887
XceI RCATGY 1 cut(s) 579
XspI CTAG 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.