FvH4_5g28990

UDP-glucose glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006844
Physical Location & Seq
Reverse (-)
20103511 .. 20124698
21188 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28990.t5

Sequence Viewer

Length: 4869 bp
ATGGGGACGCGTTTAGCCTATGCGTTTATCATTTTGATTTGTTTGATCGGCTCGGTTTCAGCTCAGAATCGGAGGCCCAAGAATGTGCAGGTCGCGGTTCGGGCCAAGTGGTCCGCTACTCCGTTGCTCCTAGAAGCTGGTGAATTGCTATCTAGAGAACATAAAGATAATTTTTGGGAATTCATTGACATCTGGCATCATAGTGATAAAGATGATCTTGATTCTTACAATGCTAAGGGTTGCCTAAAAACAATTTTGAAACATGGGCGCTCTATTTTGAGTGAACCATTGGCATCTTTGTTTGAATTCTCTCTTACATTGAGATCTGCATCCCCAAGATTGGTGCTTTACCGGCAATTAGCTGAAGAGTCTCTTTCATCTTTCCCACTGGTTGATGAAACTAATTCAAGAAGTACCAGCGGAAATTCAGAAACAAATGAACATGTTGAAATTAGAAAGTCAGATCATTTGGATGTTGGTCTAAACCCAAAAAGTCCTAATGGAAAATGTTGTTGGGTGGATACTGGAGGTGCTCTGTTCTTTGATGCTGCTGAATTAAAAAGTTGGCTTCACAGTCCTAAAGACTGTTCTAGGGATTCATTTCAGCAGCCTGAACTCTTCGAATTTGACCACATCCATTTTGATTCAACTGTTGGAAGCCCAGTTGCTGTTCTTTATGGAGCTCTTGGAACTGGTTGCTTTAGGGAGTTTCATGTCACCCTGGTTGAAGCTGCAAAAGAGGGTCATGTTAAGTATGTGGTTAGACCAGTATTACCTTCTGGGTGTGAAGCAGAAATTGACCGTTGTGGTGCTGTTGGTGCAAAAGATTCGTTAAACTTGGGTGGTTATGGTGTTGAACTTGCTTTGAAGAACATGGAATATAAGGCCATGGATGATAGTACGATAAAGAAAGGTGTCACGTTAGAAGATCCTCGGACTGAAGATCTCAGCCAAGAAGTCAGAGGGTTTATATTCTCTAAATTTCTGGAGCGTAGACCGGAACTCACATCAGAGATAATGGCTTTTAGGGATTATCTTTTGTCATCAATAATATCCGATACGCTTGATGTTTGGGAACTAAAAGATTTGGGACACCAAACTGCACAGAGGATAGTGCAAGCCACCGATCCTCTGCAGGCAATGCAGGAAATTAATCAAAACTTTCCCACTGTTGTCTCTTCCTTATCTCGCATGAAGCTTAATGATTCAGTTAAAGATGAAATTACTGCAAACCAGAGAATGATTCCACCTGGAAAGTCCTTGATGGCTATGAATGGTGCTTTAATCAATATCGAAGATGTCGACCTTTATCTGTTGGTTGACTTGGTTCATCAGGATTTATTATTGGCGGACCACTTTTCAAAGTTGAAGATACCTCATAGCACTGCACGGAAACTCCTGTCAACTCTGCCTCCTCCCGAATCTAATATGTTTCGTGTAGATTTTCGCTCGAATCATGTTCATTATCTAAATAACTTGGAAGAAGATGCTATGTATAAGCGATGGAGGAGCAATCTTAATGAGATTTTGATGCCAGTGTTCCCTGGACAGCTACGTTATATACGGAAAAACCTTTTCCATGCTGTTTCTGTTATTGATCCATCAACTGTTTGTGGTCTTCAGTCCATTGATATGCTTATTTCTTTGTATGAGAACAACTTCCCCATGAGATTTGGGGTTGTACTGTATTCTTCAAAGTTGATCAAGCACATTGAAACTAGCAGTGATGATAGTCAAATTGAGGAGGATATATCCACATCGATAATACGCCTCTTTATTTATATCAAGGAAAATCATGGAATACAAACAGCTTTCCATTTCTTGAGCAATATAAAAAAGTTGCGGGGTGAGTCTGATGGTTCGGCTGATGATCTTGAAATGCACCATGTAGAAGGAGCCTTTGTAGAAACAGTATTACCAAAGGTGAAATCTCCGCCTCAAGGCATACTGCTGAAGCTGGAGAGAGAGCAAACTTACAAGGAACGTGCACATGAAAGCACTATTTTTGTCTTTAAGCTGGGATTGGCTAAGTTGCAGTGCTGCTTGCTGATGAATGGTCTTGTGTTGGATTCTAATGAGGAGGCTCTTACAAATTCCATGAACGATGAGATGCCAAGAATACAGGAGCAAGTGTACTATGGCCATATAAATTCTCAAACAGATGTTCTTAACAAGTTTCTGTCAGAAAGTGGTACCACTCGCTATAATCCACAGATTATTGCTGGTGGAAAACCAAGGTTCACTTCTCTGTGCACATCTGTTCTTGGAGGGGAAGGTGTCTTCAATGATATTAGTTATTTGCATTCCCCTGAAACTGTGGATGATTTGAAACCTGTGACTCATCTTCTAGTGGTTGATGTTTCATCAAAGAAAGGGATGAAGCTAATTCATGAGGCCTTGCAGTATCTGATTGAAGGGTCTGATAGGGCTCGAGTAGGAGTGCTTTTCAGTGTCAATCAGGGTGCTGATCTTTCAACTCTCCTTTTTGTTGAAGTATTTCAAATCACTGCATCATTGCACAGCCACAAGAAAGATGTGTTACATTTCTTGGATCAGGTGTGTTCCTTCTTTGAACAAAATCACATGCTTGCACCATCTGAAGGTGCAGAGATCACTCAAGCGTTCATAGATAAAGTTTCTGAATTGGCCGAGAAAAATGGGTTATCATCAAAGGCCTATAAATCAGCTCTTTCAGACTTTTCTGCTGAAGAATTGAGAAAAAGGTTGAATAAGGTGGCACAGTTTTTATATAGGCAACTTGGCCTTGATTCTGGTGTTAATGGAGTCATTACTAATGGACGGGTAACGGTTGTAAATGAGGAGAGCTCATTGTTGAGCCATGATTTAAGTCTTCTTGAGTCTGTGGAGTTCACGCAGAGAATAAAGCATATTGTTGAAATAATAGAGGAAGTAAAGTGGCAGGATGTGGATCCGGATACCCTGACAAGCAAATTCATTAGTGATACTATCATGTTTGTTTCTTCTTCAATGGCTATGCGGGATCGAAGCTCTGAAGGTGCACGCTTTGAGGTTCTGAATGCAGACTATAGTGCCATCGTTCTGAACAATGAAAATTCTAGTATACATATAGATGCTGTTATTGATCCTTTAAGTCCATCTGGCCAGAAGCTATCATCAATTCTTCGGGTTCTATGGAAATATGTTCAGCCTAGCATGAGAATTGTCCTAAATCCGTTGAGTTCTCTTGTTGATCTTCCCCTGAAAAATTACTACAGATATGTAGTACCGACAGTGGATGACTTCAGCACCACTGACTACACAGTAAATGGGCCAAAGGCCTTTTTTGCAAACATGCCACTTTCCAAAACCCTCACTATGAATCTTGATGTTCCTGATCCATGGCTTGTGGAGCCTGTTATTGCAGTGCATGACTTGGATAATATTTTGCTTGAGAATCTTGGTGAGACACGAACATTACAAGCTGTTTTTGAACTTGAAGCTCTTGTTTTAACGGGTCATTGTTCTGAGAAAGATCATGATCCCCCCAGAGGCCTCCAGTTAATTATCGGTACCAAGAGTGCACCCCATTTGGTTGATACTCTTGTGATGGCCAATCTGGGATATTGGCAAATGAAAGTCTCTCCTGGTGTTTGGTACTTACAACTTGCTCCCGGAAGAAGTTCTGAGCTTTATGTCCTAAAAGATGAAGGTGATGGAAGTCAAAGCAAAACATTGTCGAAACGTATCACCATTAATGATTTGCGGGGTACAGTAGTCCACCTGGAAGTAGTAAAGAAAAAGGGGAAGGAGCATGAGAAATTGCTGCTTTCCGATGTTAATGAAAAGACACAGGATGCTACAGAGGGAAATAGCTGGAACTCCAACTTCATAAAATGGGCTTCTGGTCTTATTGGTGGCAGTGAGCACTCAAAACAGAGTGAAAATACTTCATGGGAGAAAGGAAAAGGTGGGCGCCATGGAAAAACAATAAATATTTTTTCTATTGCTTCTGGACACCTATACGAACGTTTTCTGAAAATCATGATTTTAAGTGTCTTGAAGAACACCCGCCGTCCTGTTAAGTTCTGGTTCATAAAGAACTATCTCTCTCCCCAGTTCAAGGATGTGATTCCACGTATGGCTGATGAATATGGCTTTGAGTATCAGCTAATCACCTACAAGTGGCCTACATGGTTGCACAAGCAAAAAGAAAAGCAACGAATTATTTGGGCCTATAAAATTTTGTTCCTTGATGTTATATTCCCCCTTTCTTTGGAAAAGGTCATCTTCGTTGATGCAGATCAGATCATCAGGGCAGACATGGGAGAACTCTATGATATGGATATAAAAGGAAGACCACTGGCATATACTCCCTTCTGTGACAACAACAAGGATATGGATGGATATCGGTTTTGGAGACAAGGTTTCTGGAAAGAACATTTACGGGGAAGGTCTTATCATATTAGTGCTTTGTATGTCGTTGATTTGAAGAAATTTCGAGAGACTGCAGCAGGAGATAATCTGAGAGTTTTCTATGAGACTCTTAGTAAGGACCCAAACAGTCTATCAAATTTGGACCAGGATCTGCCAAATTATGCTCAACATACAGTGCCCATATTTTCATTACCCCAAGAATGGCTTTGGTGTGAATCGTGGTGTGGTAACGCCACTAAATCCAAAGCAAAAACCATAGATCTTTGCAACAACCCAATGACAAAGGAACCCAAGCTCCAGGGTGCTAGAAGAATAGTTTCTGAGTGGCCAGATCTTGATTTGGAGGCTAGGCAGTTCACTGCGAAAATCTTAGGTGATGAAGTTGCAATTCAAGAACCACCGCCAGATCCTAATCAACCAGGGTCCGTGATGGACAGCCCACCAGAAGACCTGGAATCTAAGGCAGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003980 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006011 GO:0006139 GO:0006457 GO:0006458 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006515 GO:0006725 GO:0006793 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008194 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009626 GO:0009719 GO:0009725 GO:0009751 GO:0009812 GO:0009987 GO:0010033 GO:0010204 GO:0010243 GO:0010498 GO:0012501 GO:0012505 GO:0014070 GO:0016740 GO:0016757 GO:0016758 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0023052 GO:0030163 GO:0030968 GO:0031347 GO:0031349 GO:0033554 GO:0034050 GO:0034620 GO:0034641 GO:0034645 GO:0034976 GO:0035251 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042221 GO:0042440 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046283 GO:0046483 GO:0046527 GO:0046677 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051084 GO:0051603 GO:0051716 GO:0051788 GO:0055086 GO:0065007 GO:0070085 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0080134 GO:0097359 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Glyco_transf_8 UDP-g_GGTase

Protein Analysis

1623

Amino Acids

183.28

Weight (kDa)

5.69

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_12 PF18400 39 - 264 9.8e-51 Thioredoxin-like domain
Thioredoxin_13 PF18401 353 - 468 1.1e-33 Thioredoxin-like domain
Thioredoxin_14 PF18402 481 - 739 5.4e-58 Thioredoxin-like domain
Thioredoxin_15 PF18403 759 - 994 1.2e-41 Thioredoxin-like domain
UDP-g_GGTase PF06427 1155 - 1258 2.8e-29 UDP-glucose:Glycoprotein Glucosyltransferase
Glyco_transf_24 PF18404 1313 - 1579 2.2e-143 Glucosyltransferase 24
Glyco_transf_8 PF01501 1324 - 1544 3.3e-07 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
Acc65I GGTACC 2 cut(s) 2192, 3530
AccB1I GGYRCC 3 cut(s) 2192, 3530, 3933
AccI GTMKAC 3 cut(s) 994, 1302, 3082
AccII CGCG 2 cut(s) 10, 95
AccIII TCCGGA 1 cut(s) 2932
AclI AACGTT 1 cut(s) 3988
AcoI YGGCCR 5 cut(s) 2140, 2646, 3121, 3570, 4721
AcuI CTGAAG 8 cut(s) 384, 960, 1604, 1973, 2619, 2727, 3033, 3247
AcyI GRCGYC 1 cut(s) 3934
AfaI GTAC 9 cut(s) 415, 901, 1683, 2135, 2194, 3246, 3532, 3617, 3730
AfiI CCNNNNNNNGG 8 cut(s) 340, 1940, 2600, 3509, 4081, 4143, 4234, 4596
AflIII ACRYGT 2 cut(s) 8, 442
AjnI CCWGG 9 cut(s) 720, 1249, 1543, 3604, 3741, 4539, 4692, 4813, 4845
AjuI GAANNNNNNNTTGG 4 cut(s) 496, 528, 2227, 2259
AloI GAACNNNNNNTCC 2 cut(s) 4674, 4706
Alw21I GWGCWC 8 cut(s) 535, 685, 1990, 2255, 2828, 3022, 3544, 3888
Alw26I GTCTC 7 cut(s) 375, 1180, 3419, 3604, 4372, 4457, 4493
Alw44I GTGCAC 4 cut(s) 1986, 2251, 3018, 3540
AlwNI CAGNNNCTG 3 cut(s) 668, 2407, 4546
Ama87I CYCGRG 1 cut(s) 2430
Aor13HI TCCGGA 1 cut(s) 2932
ApaLI GTGCAC 4 cut(s) 1986, 2251, 3018, 3540
ApeKI GCWGC 6 cut(s) 548, 607, 731, 2040, 3784, 4469
AseI ATTAAT 2 cut(s) 1152, 3714
Asp700I GAANNNNTTC 5 cut(s) 1658, 2496, 3640, 3990, 4711
Asp718I GGTACC 2 cut(s) 2192, 3530
AspLEI GCGC 2 cut(s) 270, 3936
AspS9I GGNCC 9 cut(s) 75, 102, 111, 1351, 3290, 4191, 4513, 4537, 4818
AsuC2I CCSGG 1 cut(s) 3633
AsuHPI GGTGA 9 cut(s) 152, 709, 1859, 1936, 3434, 3683, 3700, 4126, 4781
AsuII TTCGAA 1 cut(s) 621
AvaI CYCGRG 1 cut(s) 2430
AvaII GGWCC 5 cut(s) 111, 1351, 4513, 4537, 4818
BaeGI GKGCMC 5 cut(s) 1990, 2255, 3022, 3544, 4575
BaeI ACNNNNGTAYC 4 cut(s) 3549, 3582, 3720, 3753
BalI TGGCCA 4 cut(s) 2142, 3123, 3572, 4723
BamHI GGATCC 1 cut(s) 2929
BanI GGYRCC 3 cut(s) 2192, 3530, 3933
BanII GRGCYC 3 cut(s) 685, 2431, 2828
BbsI GAAGAC 5 cut(s) 1610, 2272, 2843, 4321, 4848
Bbv12I GWGCWC 8 cut(s) 535, 685, 1990, 2255, 2828, 3022, 3544, 3888
BbvI GCAGC 6 cut(s) 535, 619, 718, 2027, 3771, 4481
BceAI ACGGC 1 cut(s) 4017
BcgI CGANNNNNNTGC 2 cut(s) 810, 844
BciT130I CCWGG 9 cut(s) 722, 1251, 1545, 3606, 3743, 4541, 4694, 4815, 4847
BciVI GTATCC 2 cut(s) 514, 2929
BclI TGATCA 1 cut(s) 1701
BcnI CCSGG 1 cut(s) 3633
BcoDI GTCTC 7 cut(s) 375, 1180, 3419, 3604, 4372, 4457, 4493
BfaI CTAG 9 cut(s) 131, 153, 591, 1719, 2348, 3078, 3171, 4701, 4743
BfmI CTRYAG 5 cut(s) 1133, 3046, 3232, 3819, 4467
BfoI RGCGCY 2 cut(s) 271, 3937
BfuAI ACCTGC 1 cut(s) 79
BfuI GTATCC 2 cut(s) 514, 2929
BglII AGATCT 4 cut(s) 323, 943, 4654, 4726
BisI GCNGC 6 cut(s) 549, 608, 732, 2041, 3785, 4470
BlsI GCNGC 6 cut(s) 550, 609, 733, 2042, 3786, 4471
Bme18I GGWCC 5 cut(s) 111, 1351, 4513, 4537, 4818
BmeT110I CYCGRG 1 cut(s) 2430
BmgT120I GGNCC 9 cut(s) 75, 102, 111, 1351, 3290, 4191, 4513, 4537, 4818
BmiI GGNNCC 9 cut(s) 1897, 2194, 2931, 3372, 3532, 3935, 4515, 4683, 4819
BmrI ACTGGG 2 cut(s) 656, 4069
BmuI ACTGGG 2 cut(s) 656, 4069
BpiI GAAGAC 5 cut(s) 1610, 2272, 2843, 4321, 4848
BplI GAGNNNNNCTC 2 cut(s) 2810, 2842
BpmI CTGGAG 5 cut(s) 546, 1007, 1979, 3500, 4676
Bpu10I CCTNAGC 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 621
BpuEI CTTGAG 5 cut(s) 1843, 1923, 2601, 2876, 3431
BpuMI CCSGG 1 cut(s) 3633
Bsa29I ATCGAT 1 cut(s) 1760
BsaAI YACGTR 1 cut(s) 4097
BsaBI GATNNNNATC 6 cut(s) 328, 2928, 3498, 4260, 4365, 4806
BsaHI GRCGYC 1 cut(s) 3934
BsaJI CCNNGG 9 cut(s) 720, 888, 932, 1543, 2234, 3359, 3937, 4693, 4814
BsaWI WCCGGW 2 cut(s) 997, 2932
Bsc4I CCNNNNNNNGG 8 cut(s) 340, 1940, 2600, 3509, 4081, 4143, 4234, 4596
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 9 cut(s) 393, 529, 662, 697, 767, 1535, 3517, 4075, 4326
Bse3DI GCAATG 2 cut(s) 1146, 2513
Bse8I GATNNNNATC 6 cut(s) 328, 2928, 3498, 4260, 4365, 4806
BseAI TCCGGA 1 cut(s) 2932
BseBI CCWGG 9 cut(s) 722, 1251, 1545, 3606, 3743, 4541, 4694, 4815, 4847
BseCI ATCGAT 1 cut(s) 1760
BseDI CCNNGG 9 cut(s) 720, 888, 932, 1543, 2234, 3359, 3937, 4693, 4814
BseJI GATNNNNATC 6 cut(s) 328, 2928, 3498, 4260, 4365, 4806
BseLI CCNNNNNNNGG 8 cut(s) 340, 1940, 2600, 3509, 4081, 4143, 4234, 4596
BseMI GCAATG 2 cut(s) 1146, 2513
BseMII CTCAG 6 cut(s) 77, 961, 3477, 3636, 4475, 4707
BseNI ACTGG 9 cut(s) 393, 529, 662, 697, 767, 1535, 3517, 4075, 4326
BseRI GAGGAG 5 cut(s) 1404, 1522, 1757, 2093, 2834
BseSI GKGCMC 5 cut(s) 1990, 2255, 3022, 3544, 4575
BseXI GCAGC 6 cut(s) 535, 619, 718, 2027, 3771, 4481
BseYI CCCAGC 1 cut(s) 2017
BsgI GTGCAG 4 cut(s) 107, 1086, 1371, 2625
Bsh1236I CGCG 2 cut(s) 10, 95
BshNI GGYRCC 3 cut(s) 2192, 3530, 3933
BshVI ATCGAT 1 cut(s) 1760
BsiHKAI GWGCWC 8 cut(s) 535, 685, 1990, 2255, 2828, 3022, 3544, 3888
BsiHKCI CYCGRG 1 cut(s) 2430
BsiSI CCGG 4 cut(s) 352, 998, 2933, 3633
BslFI GGGAC 2 cut(s) 19, 1104
BslI CCNNNNNNNGG 8 cut(s) 340, 1940, 2600, 3509, 4081, 4143, 4234, 4596
BsmAI GTCTC 7 cut(s) 375, 1180, 3419, 3604, 4372, 4457, 4493
BsmFI GGGAC 2 cut(s) 19, 1104
BsmI GAATGC 2 cut(s) 2302, 3043
BsoBI CYCGRG 1 cut(s) 2430
Bsp119I TTCGAA 1 cut(s) 621
Bsp13I TCCGGA 1 cut(s) 2932
Bsp19I CCATGG 3 cut(s) 888, 3359, 3937
BspCNI CTCAG 6 cut(s) 76, 960, 3478, 3637, 4476, 4708
BspDI ATCGAT 1 cut(s) 1760
BspEI TCCGGA 1 cut(s) 2932
BspFNI CGCG 2 cut(s) 10, 95
BspHI TCATGA 3 cut(s) 2389, 3496, 4002
BspLI GGNNCC 9 cut(s) 1897, 2194, 2931, 3372, 3532, 3935, 4515, 4683, 4819
BspMAI CTGCAG 2 cut(s) 1137, 4471
BspMI ACCTGC 1 cut(s) 79
BspT104I TTCGAA 1 cut(s) 621
BspT107I GGYRCC 3 cut(s) 2192, 3530, 3933
BsrDI GCAATG 2 cut(s) 1146, 2513
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 9 cut(s) 393, 529, 662, 697, 767, 1535, 3517, 4075, 4326
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 9 cut(s) 720, 888, 932, 1543, 2234, 3359, 3937, 4693, 4814
BssNAI GTATAC 1 cut(s) 3083
BssNI GRCGYC 1 cut(s) 3934
BssT1I CCWWGG 4 cut(s) 888, 2234, 3359, 3937
Bst1107I GTATAC 1 cut(s) 3083
Bst2UI CCWGG 9 cut(s) 722, 1251, 1545, 3606, 3743, 4541, 4694, 4815, 4847
Bst6I CTCTTC 3 cut(s) 360, 623, 1183
BstACI GRCGYC 1 cut(s) 3934
BstAPI GCANNNNNTGC 1 cut(s) 1141
BstBAI YACGTR 1 cut(s) 4097
BstBI TTCGAA 1 cut(s) 621
BstC8I GCNNGC 5 cut(s) 1119, 1137, 2045, 2589, 3022
BstDSI CCRYGG 3 cut(s) 888, 3359, 3937
BstFNI CGCG 2 cut(s) 10, 95
BstH2I RGCGCY 2 cut(s) 271, 3937
BstHHI GCGC 2 cut(s) 270, 3936
BstMAI GTCTC 7 cut(s) 375, 1180, 3419, 3604, 4372, 4457, 4493
BstMWI GCNNNNNNNGC 6 cut(s) 101, 352, 818, 1141, 3305, 3370
BstNI CCWGG 9 cut(s) 722, 1251, 1545, 3606, 3743, 4541, 4694, 4815, 4847
BstNSI RCATGY 3 cut(s) 446, 2587, 3316
BstSFI CTRYAG 5 cut(s) 1133, 3046, 3232, 3819, 4467
BstSLI GKGCMC 5 cut(s) 1990, 2255, 3022, 3544, 4575
BstUI CGCG 2 cut(s) 10, 95
BstV1I GCAGC 6 cut(s) 535, 619, 718, 2027, 3771, 4481
BstV2I GAAGAC 5 cut(s) 1610, 2272, 2843, 4321, 4848
BstX2I RGATCY 8 cut(s) 323, 928, 943, 2929, 4543, 4654, 4726, 4801
BstYI RGATCY 8 cut(s) 323, 928, 943, 2929, 4543, 4654, 4726, 4801
BstZ17I GTATAC 1 cut(s) 3083
Bsu15I ATCGAT 1 cut(s) 1760
BsuI GTATCC 2 cut(s) 514, 2929
BsuTUI ATCGAT 1 cut(s) 1760
BtgI CCRYGG 3 cut(s) 888, 3359, 3937
BtgZI GCGATG 1 cut(s) 1516
BtsI GCAGTG 7 cut(s) 1383, 1729, 2042, 2505, 3390, 3886, 4752
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 5 cut(s) 1119, 1137, 2045, 2589, 3022
CaiI CAGNNNCTG 3 cut(s) 668, 2407, 4546
CciI TCATGA 3 cut(s) 2389, 3496, 4002
CfoI GCGC 2 cut(s) 270, 3936
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 9 cut(s) 75, 102, 111, 1351, 3290, 4191, 4513, 4537, 4818
ClaI ATCGAT 1 cut(s) 1760
CseI GACGC 1 cut(s) 16
Csp6I GTAC 9 cut(s) 414, 900, 1682, 2134, 2193, 3245, 3531, 3616, 3729
CviQI GTAC 9 cut(s) 414, 900, 1682, 2134, 2193, 3245, 3531, 3616, 3729
DinI GGCGCC 1 cut(s) 3935
EaeI YGGCCR 5 cut(s) 2140, 2646, 3121, 3570, 4721
Eam1104I CTCTTC 3 cut(s) 360, 623, 1183
EarI CTCTTC 3 cut(s) 360, 623, 1183
EciI GGCGGA 2 cut(s) 1364, 1923
Ecl136II GAGCTC 2 cut(s) 683, 2826
Eco130I CCWWGG 4 cut(s) 888, 2234, 3359, 3937
Eco147I AGGCCT 4 cut(s) 2396, 2675, 3299, 3513
Eco24I GRGCYC 3 cut(s) 685, 2431, 2828
Eco32I GATATC 1 cut(s) 4367
Eco47I GGWCC 5 cut(s) 111, 1351, 4513, 4537, 4818
Eco53kI GAGCTC 2 cut(s) 683, 2826
Eco57I CTGAAG 8 cut(s) 384, 960, 1604, 1973, 2619, 2727, 3033, 3247
Eco88I CYCGRG 1 cut(s) 2430
EcoICRI GAGCTC 2 cut(s) 683, 2826
EcoO109I RGGNCCY 1 cut(s) 4513
EcoRI GAATTC 2 cut(s) 179, 305
EcoRII CCWGG 9 cut(s) 720, 1249, 1543, 3604, 3741, 4539, 4692, 4813, 4845
EcoRV GATATC 1 cut(s) 4367
EcoT14I CCWWGG 4 cut(s) 888, 2234, 3359, 3937
EcoT38I GRGCYC 3 cut(s) 685, 2431, 2828
EgeI GGCGCC 1 cut(s) 3935
EheI GGCGCC 1 cut(s) 3935
ErhI CCWWGG 4 cut(s) 888, 2234, 3359, 3937
FaqI GGGAC 2 cut(s) 19, 1104
FauI CCCGC 4 cut(s) 1836, 2991, 3717, 4037
FbaI TGATCA 1 cut(s) 1701
FblI GTMKAC 3 cut(s) 994, 1302, 3082
Fnu4HI GCNGC 6 cut(s) 549, 608, 732, 2041, 3785, 4470
FriOI GRGCYC 3 cut(s) 685, 2431, 2828
Fsp4HI GCNGC 6 cut(s) 549, 608, 732, 2041, 3785, 4470
FspBI CTAG 9 cut(s) 131, 153, 591, 1719, 2348, 3078, 3171, 4701, 4743
GlaI GCGC 2 cut(s) 269, 3935
GluI GCNGC 6 cut(s) 549, 608, 732, 2041, 3785, 4470
GsaI CCCAGC 1 cut(s) 2021
GsuI CTGGAG 5 cut(s) 546, 1007, 1979, 3500, 4676
HaeII RGCGCY 2 cut(s) 271, 3937
HapII CCGG 4 cut(s) 352, 998, 2933, 3633
HgaI GACGC 1 cut(s) 16
HhaI GCGC 2 cut(s) 270, 3936
Hin1I GRCGYC 1 cut(s) 3934
Hin6I GCGC 2 cut(s) 268, 3934
HinP1I GCGC 2 cut(s) 268, 3934
HincII GTYRAC 3 cut(s) 1303, 1321, 1404
HindII GTYRAC 3 cut(s) 1303, 1321, 1404
HindIII AAGCTT 1 cut(s) 1196
HpaII CCGG 4 cut(s) 352, 998, 2933, 3633
HphI GGTGA 9 cut(s) 152, 709, 1859, 1936, 3434, 3683, 3700, 4126, 4781
HpyCH4IV ACGT 6 cut(s) 920, 1555, 1984, 3703, 3988, 4096
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 352, 818, 1141, 3305, 3370
HpySE526I ACGT 6 cut(s) 920, 1555, 1984, 3703, 3988, 4096
Hsp92I GRCGYC 1 cut(s) 3934
HspAI GCGC 2 cut(s) 268, 3934
KasI GGCGCC 1 cut(s) 3933
Kpn2I TCCGGA 1 cut(s) 2932
KpnI GGTACC 2 cut(s) 2196, 3534
Ksp22I TGATCA 1 cut(s) 1701
Lsp1109I GCAGC 6 cut(s) 535, 619, 718, 2027, 3771, 4481
MaeI CTAG 9 cut(s) 131, 153, 591, 1719, 2348, 3078, 3171, 4701, 4743
MaeII ACGT 6 cut(s) 920, 1555, 1984, 3703, 3988, 4096
MaeIII GTNAC 7 cut(s) 715, 916, 2335, 2538, 2803, 4340, 4622
MflI RGATCY 8 cut(s) 323, 928, 943, 2929, 4543, 4654, 4726, 4801
MlsI TGGCCA 4 cut(s) 2142, 3123, 3572, 4723
MluI ACGCGT 1 cut(s) 8
MluNI TGGCCA 4 cut(s) 2142, 3123, 3572, 4723
Mly113I GGCGCC 1 cut(s) 3934
MlyI GAGTC 6 cut(s) 377, 1859, 2332, 2793, 2867, 4495
MmeI TCCRAC 3 cut(s) 634, 2046, 3867
Mox20I TGGCCA 4 cut(s) 2142, 3123, 3572, 4723
MroI TCCGGA 1 cut(s) 2932
MroXI GAANNNNTTC 5 cut(s) 1658, 2496, 3640, 3990, 4711
MscI TGGCCA 4 cut(s) 2142, 3123, 3572, 4723
MslI CAYNNNNRTG 5 cut(s) 201, 471, 1631, 3090, 4425
Msp20I TGGCCA 4 cut(s) 2142, 3123, 3572, 4723
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 4 cut(s) 352, 998, 2933, 3633
Mva1269I GAATGC 2 cut(s) 2302, 3043
MvaI CCWGG 9 cut(s) 722, 1251, 1545, 3606, 3743, 4541, 4694, 4815, 4847
MvnI CGCG 2 cut(s) 10, 95
MwoI GCNNNNNNNGC 6 cut(s) 101, 352, 818, 1141, 3305, 3370
NarI GGCGCC 1 cut(s) 3934
NciI CCSGG 1 cut(s) 3633
NcoI CCATGG 3 cut(s) 888, 3359, 3937
NlaIV GGNNCC 9 cut(s) 1897, 2194, 2931, 3372, 3532, 3935, 4515, 4683, 4819
NmeAIII GCCGAG 1 cut(s) 2674
NmuCI GTSAC 4 cut(s) 715, 916, 2335, 4340
NspI RCATGY 3 cut(s) 446, 2587, 3316
NspV TTCGAA 1 cut(s) 621
PaeR7I CTCGAG 1 cut(s) 2430
PagI TCATGA 3 cut(s) 2389, 3496, 4002
PceI AGGCCT 4 cut(s) 2396, 2675, 3299, 3513
PciI ACATGT 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 1299
PctI GAATGC 2 cut(s) 2302, 3043
PdmI GAANNNNTTC 5 cut(s) 1658, 2496, 3640, 3990, 4711
PfoI TCCNGGA 1 cut(s) 3631
PkrI GCNGC 6 cut(s) 550, 609, 733, 2042, 3786, 4471
PleI GAGTC 6 cut(s) 376, 1858, 2332, 2792, 2866, 4495
PluTI GGCGCC 1 cut(s) 3937
PpsI GAGTC 6 cut(s) 376, 1858, 2332, 2792, 2866, 4495
Ppu21I YACGTR 1 cut(s) 4097
PpuMI RGGWCCY 1 cut(s) 4513
PscI ACATGT 1 cut(s) 442
PshBI ATTAAT 2 cut(s) 1152, 3714
Psp124BI GAGCTC 2 cut(s) 685, 2828
Psp1406I AACGTT 1 cut(s) 3988
Psp5II RGGWCCY 1 cut(s) 4513
Psp6I CCWGG 9 cut(s) 720, 1249, 1543, 3604, 3741, 4539, 4692, 4813, 4845
PspFI CCCAGC 1 cut(s) 2017
PspGI CCWGG 9 cut(s) 720, 1249, 1543, 3604, 3741, 4539, 4692, 4813, 4845
PspN4I GGNNCC 9 cut(s) 1897, 2194, 2931, 3372, 3532, 3935, 4515, 4683, 4819
PspPI GGNCC 9 cut(s) 75, 102, 111, 1351, 3290, 4191, 4513, 4537, 4818
PspPPI RGGWCCY 1 cut(s) 4513
PspXI VCTCGAGB 1 cut(s) 2430
PstI CTGCAG 2 cut(s) 1137, 4471
PstNI CAGNNNCTG 3 cut(s) 668, 2407, 4546
PsuI RGATCY 8 cut(s) 323, 928, 943, 2929, 4543, 4654, 4726, 4801
RsaI GTAC 9 cut(s) 415, 901, 1683, 2135, 2194, 3246, 3532, 3617, 3730
RsaNI GTAC 9 cut(s) 414, 900, 1682, 2134, 2193, 3245, 3531, 3616, 3729
RseI CAYNNNNRTG 5 cut(s) 201, 471, 1631, 3090, 4425
SacI GAGCTC 2 cut(s) 685, 2828
SalI GTCGAC 1 cut(s) 1301
SatI GCNGC 6 cut(s) 549, 608, 732, 2041, 3785, 4470
Sau96I GGNCC 9 cut(s) 75, 102, 111, 1351, 3290, 4191, 4513, 4537, 4818
SchI GAGTC 6 cut(s) 377, 1859, 2332, 2793, 2867, 4495
SfcI CTRYAG 5 cut(s) 1133, 3046, 3232, 3819, 4467
SfoI GGCGCC 1 cut(s) 3935
Sfr274I CTCGAG 1 cut(s) 2430
SfuI TTCGAA 1 cut(s) 621
SinI GGWCC 5 cut(s) 111, 1351, 4513, 4537, 4818
SlaI CTCGAG 1 cut(s) 2430
SmiMI CAYNNNNRTG 5 cut(s) 201, 471, 1631, 3090, 4425
SmlI CTYRAG 6 cut(s) 1822, 1938, 2430, 2616, 2855, 3410
SmoI CTYRAG 6 cut(s) 1822, 1938, 2430, 2616, 2855, 3410
SseBI AGGCCT 4 cut(s) 2396, 2675, 3299, 3513
SspDI GGCGCC 1 cut(s) 3933
SspI AATATT 2 cut(s) 3403, 3955
SspMI CTAG 9 cut(s) 131, 153, 591, 1719, 2348, 3078, 3171, 4701, 4743
SstI GAGCTC 2 cut(s) 685, 2828
StuI AGGCCT 4 cut(s) 2396, 2675, 3299, 3513
StyI CCWWGG 4 cut(s) 888, 2234, 3359, 3937
TaiI ACGT 6 cut(s) 923, 1558, 1987, 3706, 3991, 4099
TaqI TCGA 9 cut(s) 621, 1293, 1302, 1451, 1760, 2431, 3004, 3698, 4459
TatI WGTACW 2 cut(s) 1681, 2133
TseFI GTSAC 4 cut(s) 715, 916, 2335, 4340
TseI GCWGC 6 cut(s) 548, 607, 731, 2040, 3784, 4469
Tsp45I GTSAC 4 cut(s) 715, 916, 2335, 4340
TspGWI ACGGA 5 cut(s) 111, 1405, 1579, 3183, 4810
VneI GTGCAC 4 cut(s) 1986, 2251, 3018, 3540
VpaK11BI GGWCC 5 cut(s) 111, 1351, 4513, 4537, 4818
VspI ATTAAT 2 cut(s) 1152, 3714
XbaI TCTAGA 1 cut(s) 152
XceI RCATGY 3 cut(s) 446, 2587, 3316
XhoI CTCGAG 1 cut(s) 2430
XmiI GTMKAC 3 cut(s) 994, 1302, 3082
XmnI GAANNNNTTC 5 cut(s) 1658, 2496, 3640, 3990, 4711
XspI CTAG 9 cut(s) 131, 153, 591, 1719, 2348, 3078, 3171, 4701, 4743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.