MD08G1235900.v1.1

UDP-glucose glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006844
Physical Location & Seq
Reverse (-)
30342962 .. 30370217
27256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1235900.v1.1.491

Sequence Viewer

Length: 4950 bp
ATGCTTCTGAAGGATCCGAACCCGTTCTGTTTCGTTCGGAAACTGCAAATGGGGACCCGTTATAAATCTGCGTTTTGTGCTGTGATCGTCTTGGTGTGTTTGGGAGCGTCAGGAATCGGTTTGGTTTCGGCTCAGAATCGGAGGCCTAAGAATGTTCAAGCTGCGGTTCGGGCCAAGTGGTCGGGTACTCCATTGCTCCTGGAAGCCGGTGAATTACTATCTAAAGAACAAAAAGACCATTTTTGGGACTTCATTGATGCCTGGCATAATAGTGAGAAGGATGATGCTGAATCTTATACTGCTAAAGGCTGCCTAAAAAAAATTGTAAAGCACGGACTCTCTATTTTGAACAAACCATTGGCATCTTTGTTTGAATTCTCTCTTATGATGAGATCTACATCTCCAAGATTGGTTCTTTATAGGCAATTAGCTGCAGAGTCTCTGTCTTCTTTTCCTCCGGTTGATGAAACTAATTCAAGCAATGACAGTGGAATTTCTGAAACAAATGAACATGTGGAAAGTAAAAGGTCAGATCCCTTGAACATTGGTCGAAACCCGAAAAGCCCTAATGGGAAATGTTGTTGGGTGGATACTGGAGGTGCACTGTTCCTTGATCCAGCAGACTTGAAAATTTGGCTTCAAAGTCCAAGAGATTCTTCTGGAGATTCATTTCAGCAGCCTGAACTTTTCGAATTTGATCACATCCATTTTGATTCAAGTGTTGGAAGTCCAGTTGCTGTTCTTTATGGAGCTCTTGGAACTGATTGCTTTAGGGCGTTCCATCTCACCCTAGTTGAAGCTGCAAAAGAGGGAAAAGTTAAGTACGTCGTTAGACAAGTATTACCTTCTGGCTGTGATGTGGAGATTGAACGTTGTGGTGCTGTTGGTACAAGAGATTCGTTAAACTTAGGAGGCTATGGAGTGGAACTTGCTTTGAAGAATATGGAATATAAGGCAATGGATGATAGTGCTATAAAGAAAGGTGTCACACTAGAAGATCCTAGGATTGAAGATCTCAGCCAAGAAGTCAGAGGGTTTATATTCTCCAAAATTCTGGAACGCAAACCTGAACTCACTTCAGAGATAATGACATTCAGGGATTATCTTTTGTCATCAACAATATCTGATACACTTGATGTTTGGGAACTAAAAGATTTAGGACATCAAACTGCACAGAGGATAGTTCAAGCCTCTGATCCTTTGCAAGCAATGCAGGAAATTAATCAAAATTTCCCGAGCATTGTCTCTTCCTTATCTCGCATGAAGCTCAATGATTCAGTTAAAGATGAAATAAGTGCAAACCAGAGAATGATCCCACCTGGCAAGTCCTTAATGGCTCTGAATGGTGCTTTACTCAATATTGAAGATATTGACCTTTATCTGTTGCTTGACTTGGTTCATCAAGATTTATCATTGGCTTATCAGTTTTCAAAGTTGAAGATTCCTCATAGCACTGTACGGAAACTCCTTGCAAGTCTGCCTCCTCCAGAATCTAATATGCTCCGTGTAGATTTTCGTTCAAATCATGTTCAATATCTAAATAACATAGAAGAAGATGCTATGTATAGGCGATGGAGGAATAATCTAAATGAGATTTTGATGCCAGTGTTCCCCGGTCAGATACGTTATATCCGGAAAAACCTTTACCATGCAGTTTCTGTTATTGATCCATCAACTGTTTGCGGTCTTCAGTCCATTGACATGATTTTGTCTTTGTACGAGAATAATTTTCCTATGAGATTTGGAGTTGTACTGTATTCTTCGAAGTTCATCAATCATATCGAAACTAGTGGCAGTGAAGATGACCATGAAATTGAGGAGGATATGTCCAGCTTCATTATACGTCTCTTCATTTATATAAAGGAGAATCATGGAATTCAAACAGCTTTCCAGTTTTTGAGCAACATAAACAAATTGCGGATTGATTCTGAAGATTCTGCCGATGATGCTCTTGAAACGCACCACGTGGAAGGAGCATTTGTAGAAACTGTATTGCCCAAAGCAAAATCTCCACCTCATGATTTATTGCTAAAGCTGGAGAAGGAGCAAACTTTCAAGGAACTTTCTCAAGAAAGCTCCATGTTTATCCTTAAGCTGGGTTTGGCTAAGCTACAGTGTTGCTTGTTGATGAATGGGCTTGTGTTGGAATCGAATGAGGAGGCTCTTATAAACTCCATGAATGATGAGCTGCCCAGAATACAGGAGCAAGTGTACTATGGGCATATAAATTCGCGAACAGATGTTCTTGATAAGTTTCTGTCAGAAAGTGGTACCACTCGCTATAATCCACAGATTATTGCTGGTGGAAAGCCAAGGTTCATTTCTCTGTCTACATCTGTTTTTGGAGGGGAAGGTGTCTTAAATGATATTAACTATTTGCATTCTCCCGAAACTATGGATGATTTGAAGCCCGTGACCCATCTTCTAGCTGTTAATATTGCATCAAAGAAAGGGACGAAGCTGCTTCGTGAAGGCTTGCACTATCTGATAGAAGCGTCCAATCGTGCTCGAGTAGGTGTGCTTTTCAGTGTCAATCAGGATGCTGATGTCTCTAGGCATCTTTTTGTGAAGGTGTTTGAAATCACTGCATCCTTGTATAGCCATAAGAAAAAGGTGTTAGATTTCCTGGATCAGATGTGCTCATTCTATGATCATAATTATTTGCTTCCATCATCTAAGGGTGCTGAGAGCACTCAAGCATTTATAGATAAAGTCTGTGAATTGGCTGAGGCTAATGGATTGTCATCAAAGGCCTATAGATTTGCTCCATCTGAATTTTCTGATGACAAATTGAGAAAATATATGAATAAGGTATCCCATTTTTTTTATAGGCAGCTTGGCATTGAAACTGGTGTTAATGCAGTCATTACTAATGGAAGGGTGACACTTGTAAATGACGGGGGCACATTCTTGAGCCATGATTTACGTCTTCTAGAGTCCCTAGAGTTTGCCCAGAGAATAAAGCACATTGTGGAAATTATTGAAGAAGTGAAGTGGCAGGATGTGGATCCCGACATCCTAACAAGCAAATTCATTAGTGATACTATCATGCTTGTGTCATCTTCCATGGCTATGCGGGATCGAAGTTCTGAAAGTGCCCGCTTTGACATTTTGAATGCACAATATAGTGCCATTGTTTTGAACAATGAAAATGCTAGTATTCATATCGATGCAGTTGTTGATCCTTTAAGTCCATTTGGGCAGAAGCTATCATCAATTCTTCGAGTTCTATGGAAATATGTTCAGCCTAGCATGAGGATTGTACTAAATCCGTTGAGTTCTCTTATTGATCTTCCTCTGAAGAATTACTACAGATACGTCCTACCAACAGTGGATGACTTCAGCAGCACTGACTACACAATAAATGGGCCAAAAGCTTTTTTTGCAAACATGCCACTGTCCAAAACTCTCACGATGAATCTTGATGTTCCTGATCCATGGCTAGTTGAGCCTGTTATTGCAGTCCATGACTTGGATAATATTTTGCTTGAGAATCTTGGTGAGACACGGACATTACAAGCTGTTTTTGAACTTGAAGCTCTTGTTCTGACGGGTCATTGTTCTGAGAAAGATCATGATCCTCCCCGAGGCCTTCAGTTGATTATTGGTACTAAGAGTACTCCTCACTTGGTTGATACTCTTGTGATGGCCAATCTGGGTTATTGGCAAATGAAAGTGTCTCCTGGGGTTTGGTACTTGCAACTTGCTCCAGGTAGAAGCTCTGAACTTTATTTTCTGAAAGAGAATGGTGAAGGAAGTGGGAGCAAAACATTATCCAAACGTATCACCATAGACGATTTACGGGGTAACGTAGTTCACATGGAAGTAGCAAAGAAAAAGGGAAAGGAGCATGAGAAACTGCTGGTTCCTGATGGCGAAGACAACTCACACGATAATAAGGAGGGAAGTAGCTGGAACTCCAACTTCTTGAAATGGGCTTCTGGTTTTATTGGTGGCAGTGAGCAATCTAAAAAGAGTGAAAGTACTTCAGCGGAGCAAGGAAAGGGTGGACGACATGGAAAAACGATTAATATTTTCTCTATTGCTTCTGGACACCTATATGAACGTTTTCTGAAAATCATGATTTTAAGTGTCTTGAAGAACACCCGCCGTCCAGTCAAATTCTGGTTCATAAAGAACTATCTCTCTCCCCAGTTCAAGGATGTCATTCCACCTATGGCTCAAGAATATGGTTTTGAGTATGAACTAATAACGTACAAGTGGCCTACATGGTTGCATAAGCAGAAAGAAAAGCAACGGATTATTTGGGCCTATAAAATCTTGTTCCTTGACGTTATCTTTCCCCTTTCATTGGAAAAGGTCATTTTTGTTGATGCAGATCAAATTGTAAGGGCAGACATGGGAGAGCTCTATGACATGGATATAAAAGGACGACCTCTGGCATATACTCCATTTTGTGACAACAACAGGGATATGGATGGATATCGGTTTTGGAGACAAGGTTTCTGGAAAGAACATTTACGGGGGAGATCCTACCACATAAGTGCTTTATATGTTGTCAATTTGAAGAAATTTCGAGAGACTGCAGCAGGAGATAATCTGAGAGTTTTCTACGAGACTCTTAGCAAGGACCCAAACAGTTTATCTAATTTGGACCAGGATCTTCCAAACTATGCTCAACATACGGTACCCATCTTTTCATTACCGCAAGAATGGTTGTGGTGCGAGTCATGGTGCGGTAATGCAACAAAATCCAAAGCAAAAACCATTGATCTTTGCAACAACCCCATGACAAAGGAACCCAAACTTCAGGGGGCTCGAAGAATAGTTTCTGAATGGCCAGATCTTGATTTGGAGGCAAGGCAATTCACGGCGAAGATCTTAGGTGACGAACTGGACATCCAAGAGCCCGCACCACTTCCTAGTCCATCAGAGAAATCAGTGACTGGCAGTCCGGAGGAGGACCTTGAATCCAAGGCAGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003980 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006011 GO:0006139 GO:0006457 GO:0006458 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006515 GO:0006725 GO:0006793 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008194 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009626 GO:0009719 GO:0009725 GO:0009751 GO:0009812 GO:0009987 GO:0010033 GO:0010204 GO:0010243 GO:0010498 GO:0012501 GO:0012505 GO:0014070 GO:0016740 GO:0016757 GO:0016758 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0023052 GO:0030163 GO:0030968 GO:0031347 GO:0031349 GO:0033554 GO:0034050 GO:0034620 GO:0034641 GO:0034645 GO:0034976 GO:0035251 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042221 GO:0042440 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046283 GO:0046483 GO:0046527 GO:0046677 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051084 GO:0051603 GO:0051716 GO:0051788 GO:0055086 GO:0065007 GO:0070085 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0080134 GO:0097359 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Glyco_transf_8 UDP-g_GGTase

Protein Analysis

1650

Amino Acids

186.44

Weight (kDa)

5.79

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_12 PF18400 63 - 286 6.5e-50 Thioredoxin-like domain
Thioredoxin_13 PF18401 375 - 491 7.6e-34 Thioredoxin-like domain
Thioredoxin_14 PF18402 504 - 765 1.7e-57 Thioredoxin-like domain
Thioredoxin_15 PF18403 786 - 1024 9.3e-38 Thioredoxin-like domain
UDP-g_GGTase PF06427 1185 - 1288 3.1e-29 UDP-glucose:Glycoprotein Glucosyltransferase
Glyco_transf_24 PF18404 1343 - 1609 4.3e-143 Glucosyltransferase 24
Glyco_transf_8 PF01501 1354 - 1574 1.5e-07 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 63, 2168
Acc65I GGTACC 2 cut(s) 2270, 4648
AccB1I GGYRCC 2 cut(s) 2270, 4648
AccB7I CCANNNNNTGG 1 cut(s) 3472
AccI GTMKAC 1 cut(s) 2330
AccII CGCG 1 cut(s) 2233
AccIII TCCGGA 2 cut(s) 1632, 4915
AclI AACGTT 2 cut(s) 871, 4066
AcoI YGGCCR 2 cut(s) 3648, 4799
AcuI CTGAAG 9 cut(s) 29, 1062, 1673, 1950, 3320, 3325, 3578, 3972, 4754
AcvI CACGTG 1 cut(s) 1966
AdeI CACNNNGTG 2 cut(s) 1966, 2971
AfiI CCNNNNNNNGG 6 cut(s) 244, 2095, 3472, 3587, 4159, 4312
AflII CTTAAG 1 cut(s) 2090
AflIII ACRYGT 1 cut(s) 511
AhdI GACNNNNNGTC 1 cut(s) 4911
AhlI ACTAGT 1 cut(s) 1787
AjnI CCWGG 7 cut(s) 198, 260, 1318, 2625, 3682, 3709, 4617
AjuI GAANNNNNNNTTGG 6 cut(s) 341, 373, 640, 672, 2305, 2337
AleI CACNNNNGTG 1 cut(s) 4503
AloI GAACNNNNNNTCC 2 cut(s) 4845, 4877
Alw21I GWGCWC 6 cut(s) 604, 754, 2509, 2642, 2693, 4371
Alw26I GTCTC 9 cut(s) 444, 1249, 1850, 2554, 3497, 3684, 4450, 4535, 4571
Alw44I GTGCAC 1 cut(s) 600
AlwNI CAGNNNCTG 3 cut(s) 737, 1658, 4907
Ama87I CYCGRG 3 cut(s) 1234, 2508, 3585
Aor13HI TCCGGA 2 cut(s) 1632, 4915
AoxI GGCC 9 cut(s) 143, 171, 2751, 3368, 3589, 3648, 4223, 4269, 4799
ApaLI GTGCAC 1 cut(s) 600
AseI ATTAAT 2 cut(s) 1221, 4029
Asp700I GAANNNNTTC 3 cut(s) 23, 4068, 4789
Asp718I GGTACC 2 cut(s) 2270, 4648
AspA2I CCTAGG 1 cut(s) 1001
AspS9I GGNCC 7 cut(s) 54, 171, 3368, 4269, 4591, 4615, 4924
AsuC2I CCSGG 1 cut(s) 1614
AsuHPI GGTGA 7 cut(s) 221, 778, 2893, 3512, 3761, 3778, 4859
AsuII TTCGAA 2 cut(s) 690, 1763
AvaI CYCGRG 3 cut(s) 1234, 2508, 3585
AvaII GGWCC 4 cut(s) 54, 4591, 4615, 4924
AvrII CCTAGG 1 cut(s) 1001
BaeGI GKGCMC 3 cut(s) 604, 2906, 3100
BaeI ACNNNNGTAYC 2 cut(s) 3627, 3660
BalI TGGCCA 2 cut(s) 3650, 4801
BamHI GGATCC 2 cut(s) 13, 3007
BanI GGYRCC 2 cut(s) 2270, 4648
BanII GRGCYC 4 cut(s) 754, 4371, 4780, 4872
BarI GAAGNNNNNNTAC 2 cut(s) 3293, 3325
BbrPI CACGTG 1 cut(s) 1966
BbsI GAAGAC 4 cut(s) 438, 1679, 2921, 3885
Bbv12I GWGCWC 6 cut(s) 604, 754, 2509, 2642, 2693, 4371
BbvCI CCTCAGC 1 cut(s) 2727
BceAI ACGGC 2 cut(s) 4095, 4848
BciT130I CCWGG 7 cut(s) 200, 262, 1320, 2627, 3684, 3711, 4619
BciVI GTATCC 2 cut(s) 583, 2824
BclI TGATCA 2 cut(s) 697, 2650
BcnI CCSGG 1 cut(s) 1614
BcoDI GTCTC 9 cut(s) 444, 1249, 1850, 2554, 3497, 3684, 4450, 4535, 4571
BcuI ACTAGT 1 cut(s) 1787
BfmI CTRYAG 5 cut(s) 432, 2111, 2755, 3310, 4545
BfrI CTTAAG 1 cut(s) 2090
BfuI GTATCC 2 cut(s) 583, 2824
BglII AGATCT 4 cut(s) 392, 1012, 4804, 4839
BlnI CCTAGG 1 cut(s) 1001
BlpI GCTNAGC 1 cut(s) 2106
BmcAI AGTACT 2 cut(s) 3619, 3985
Bme1390I CCNGG 8 cut(s) 200, 262, 1320, 1614, 2627, 3684, 3711, 4619
Bme18I GGWCC 4 cut(s) 54, 4591, 4615, 4924
BmeRI GACNNNNNGTC 1 cut(s) 4911
BmeT110I CYCGRG 3 cut(s) 1234, 2508, 3585
BmgT120I GGNCC 7 cut(s) 54, 171, 3368, 4269, 4591, 4615, 4924
BmiI GGNNCC 9 cut(s) 15, 55, 56, 2272, 3009, 3867, 4593, 4650, 4761
BmrFI CCNGG 8 cut(s) 200, 262, 1320, 1614, 2627, 3684, 3711, 4619
BmrI ACTGGG 1 cut(s) 4147
BmuI ACTGGG 1 cut(s) 4147
BpiI GAAGAC 4 cut(s) 438, 1679, 2921, 3885
BplI GAGNNNNNCTC 2 cut(s) 3607, 3639
BpmI CTGGAG 5 cut(s) 615, 681, 1470, 2057, 3693
Bpu10I CCTNAGC 1 cut(s) 2727
Bpu1102I GCTNAGC 1 cut(s) 2106
Bpu14I TTCGAA 2 cut(s) 690, 1763
BpuEI CTTGAG 5 cut(s) 2052, 2679, 2932, 3509, 4167
BpuMI CCSGG 1 cut(s) 1614
Bsa29I ATCGAT 1 cut(s) 3168
BsaAI YACGTR 1 cut(s) 1966
BsaBI GATNNNNATC 6 cut(s) 397, 2743, 3006, 3576, 4338, 4443
BsaJI CCNNGG 8 cut(s) 1001, 1612, 2312, 3066, 3437, 3586, 3683, 4935
BsaWI WCCGGW 3 cut(s) 457, 1632, 4915
Bsc4I CCNNNNNNNGG 6 cut(s) 244, 2095, 3472, 3587, 4159, 4312
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 9 cut(s) 598, 731, 1604, 1891, 2854, 4115, 4153, 4860, 4912
Bse3DI GCAATG 4 cut(s) 191, 487, 963, 1215
Bse8I GATNNNNATC 6 cut(s) 397, 2743, 3006, 3576, 4338, 4443
BseAI TCCGGA 2 cut(s) 1632, 4915
BseBI CCWGG 7 cut(s) 200, 262, 1320, 2627, 3684, 3711, 4619
BseCI ATCGAT 1 cut(s) 3168
BseDI CCNNGG 8 cut(s) 1001, 1612, 2312, 3066, 3437, 3586, 3683, 4935
BseJI GATNNNNATC 6 cut(s) 397, 2743, 3006, 3576, 4338, 4443
BseLI CCNNNNNNNGG 6 cut(s) 244, 2095, 3472, 3587, 4159, 4312
BseMI GCAATG 4 cut(s) 191, 487, 963, 1215
BseMII CTCAG 6 cut(s) 146, 1030, 2676, 2718, 3555, 4553
BseNI ACTGG 9 cut(s) 598, 731, 1604, 1891, 2854, 4115, 4153, 4860, 4912
BseRI GAGGAG 5 cut(s) 1473, 1832, 2171, 3612, 4934
BseSI GKGCMC 3 cut(s) 604, 2906, 3100
BseYI CCCAGC 1 cut(s) 2095
BsgI GTGCAG 1 cut(s) 1155
Bsh1236I CGCG 1 cut(s) 2233
BshFI GGCC 9 cut(s) 145, 173, 2753, 3370, 3591, 3650, 4225, 4271, 4801
BshNI GGYRCC 2 cut(s) 2270, 4648
BshVI ATCGAT 1 cut(s) 3168
BsiHKAI GWGCWC 6 cut(s) 604, 754, 2509, 2642, 2693, 4371
BsiHKCI CYCGRG 3 cut(s) 1234, 2508, 3585
BsiSI CCGG 5 cut(s) 207, 458, 1614, 1633, 4916
BslFI GGGAC 4 cut(s) 67, 260, 2467, 2923
BslI CCNNNNNNNGG 6 cut(s) 244, 2095, 3472, 3587, 4159, 4312
BsmAI GTCTC 9 cut(s) 444, 1249, 1850, 2554, 3497, 3684, 4450, 4535, 4571
BsmBI CGTCTC 1 cut(s) 1850
BsmFI GGGAC 4 cut(s) 67, 260, 2467, 2923
BsmI GAATGC 2 cut(s) 2380, 3121
BsnI GGCC 9 cut(s) 145, 173, 2753, 3370, 3591, 3650, 4225, 4271, 4801
BsoBI CYCGRG 3 cut(s) 1234, 2508, 3585
Bsp119I TTCGAA 2 cut(s) 690, 1763
Bsp13I TCCGGA 2 cut(s) 1632, 4915
Bsp1720I GCTNAGC 1 cut(s) 2106
Bsp19I CCATGG 2 cut(s) 3066, 3437
Bsp68I TCGCGA 1 cut(s) 2233
BspANI GGCC 9 cut(s) 145, 173, 2753, 3370, 3591, 3650, 4225, 4271, 4801
BspCNI CTCAG 6 cut(s) 145, 1029, 2677, 2719, 3556, 4554
BspDI ATCGAT 1 cut(s) 3168
BspEI TCCGGA 2 cut(s) 1632, 4915
BspFNI CGCG 1 cut(s) 2233
BspHI TCATGA 3 cut(s) 2017, 3574, 4080
BspLI GGNNCC 9 cut(s) 15, 55, 56, 2272, 3009, 3867, 4593, 4650, 4761
BspMAI CTGCAG 2 cut(s) 436, 4549
BspT104I TTCGAA 2 cut(s) 690, 1763
BspT107I GGYRCC 2 cut(s) 2270, 4648
BspTI CTTAAG 1 cut(s) 2090
BsrDI GCAATG 4 cut(s) 191, 487, 963, 1215
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 9 cut(s) 598, 731, 1604, 1891, 2854, 4115, 4153, 4860, 4912
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 8 cut(s) 1001, 1612, 2312, 3066, 3437, 3586, 3683, 4935
BssT1I CCWWGG 5 cut(s) 1001, 2312, 3066, 3437, 4935
Bst2UI CCWGG 7 cut(s) 200, 262, 1320, 2627, 3684, 3711, 4619
Bst6I CTCTTC 2 cut(s) 1252, 1853
BstAFI CTTAAG 1 cut(s) 2090
BstAPI GCANNNNNTGC 1 cut(s) 1210
BstBAI YACGTR 1 cut(s) 1966
BstBI TTCGAA 2 cut(s) 690, 1763
BstC8I GCNNGC 4 cut(s) 1206, 2477, 3100, 4872
BstDSI CCRYGG 2 cut(s) 3066, 3437
BstENI CCTNNNNNAGG 1 cut(s) 3585
BstFNI CGCG 1 cut(s) 2233
BstMAI GTCTC 9 cut(s) 444, 1249, 1850, 2554, 3497, 3684, 4450, 4535, 4571
BstMWI GCNNNNNNNGC 6 cut(s) 77, 170, 1210, 1946, 3383, 3448
BstNI CCWGG 7 cut(s) 200, 262, 1320, 2627, 3684, 3711, 4619
BstNSI RCATGY 2 cut(s) 515, 3394
BstSCI CCNGG 8 cut(s) 198, 260, 1318, 1612, 2625, 3682, 3709, 4617
BstSFI CTRYAG 5 cut(s) 432, 2111, 2755, 3310, 4545
BstSLI GKGCMC 3 cut(s) 604, 2906, 3100
BstUI CGCG 1 cut(s) 2233
BstV2I GAAGAC 4 cut(s) 438, 1679, 2921, 3885
BstXI CCANNNNNNTGG 1 cut(s) 1054
Bsu15I ATCGAT 1 cut(s) 3168
BsuI GTATCC 2 cut(s) 583, 2824
BsuRI GGCC 9 cut(s) 145, 173, 2753, 3370, 3591, 3650, 4225, 4271, 4801
BsuTUI ATCGAT 1 cut(s) 3168
BtgI CCRYGG 2 cut(s) 3066, 3437
BtgZI GCGATG 1 cut(s) 1585
BtsI GCAGTG 3 cut(s) 1801, 2583, 3964
BtuMI TCGCGA 1 cut(s) 2233
Cac8I GCNNGC 4 cut(s) 1206, 2477, 3100, 4872
CaiI CAGNNNCTG 3 cut(s) 737, 1658, 4907
CciI TCATGA 3 cut(s) 2017, 3574, 4080
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 7 cut(s) 54, 171, 3368, 4269, 4591, 4615, 4924
ClaI ATCGAT 1 cut(s) 3168
CseI GACGC 2 cut(s) 96, 2484
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
DraIII CACNNNGTG 2 cut(s) 1966, 2971
DriI GACNNNNNGTC 1 cut(s) 4911
EaeI YGGCCR 2 cut(s) 3648, 4799
Eam1104I CTCTTC 2 cut(s) 1252, 1853
Eam1105I GACNNNNNGTC 1 cut(s) 4911
EarI CTCTTC 2 cut(s) 1252, 1853
Ecl136II GAGCTC 2 cut(s) 752, 4369
Eco130I CCWWGG 5 cut(s) 1001, 2312, 3066, 3437, 4935
Eco147I AGGCCT 3 cut(s) 145, 2753, 3591
Eco24I GRGCYC 4 cut(s) 754, 4371, 4780, 4872
Eco32I GATATC 1 cut(s) 4445
Eco47I GGWCC 4 cut(s) 54, 4591, 4615, 4924
Eco53kI GAGCTC 2 cut(s) 752, 4369
Eco57I CTGAAG 9 cut(s) 29, 1062, 1673, 1950, 3320, 3325, 3578, 3972, 4754
Eco72I CACGTG 1 cut(s) 1966
Eco88I CYCGRG 3 cut(s) 1234, 2508, 3585
EcoICRI GAGCTC 2 cut(s) 752, 4369
EcoNI CCTNNNNNAGG 1 cut(s) 3585
EcoO109I RGGNCCY 3 cut(s) 54, 4591, 4924
EcoRI GAATTC 2 cut(s) 374, 1875
EcoRII CCWGG 7 cut(s) 198, 260, 1318, 2625, 3682, 3709, 4617
EcoRV GATATC 1 cut(s) 4445
EcoT14I CCWWGG 5 cut(s) 1001, 2312, 3066, 3437, 4935
EcoT38I GRGCYC 4 cut(s) 754, 4371, 4780, 4872
ErhI CCWWGG 5 cut(s) 1001, 2312, 3066, 3437, 4935
Esp3I CGTCTC 1 cut(s) 1850
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FaqI GGGAC 4 cut(s) 67, 260, 2467, 2923
FauI CCCGC 4 cut(s) 3069, 3107, 4115, 4879
FbaI TGATCA 2 cut(s) 697, 2650
FblI GTMKAC 1 cut(s) 2330
FriOI GRGCYC 4 cut(s) 754, 4371, 4780, 4872
GsaI CCCAGC 1 cut(s) 2099
GsuI CTGGAG 5 cut(s) 615, 681, 1470, 2057, 3693
HaeIII GGCC 9 cut(s) 145, 173, 2753, 3370, 3591, 3650, 4225, 4271, 4801
HapII CCGG 5 cut(s) 207, 458, 1614, 1633, 4916
HgaI GACGC 2 cut(s) 96, 2484
HindIII AAGCTT 1 cut(s) 3375
HpaII CCGG 5 cut(s) 207, 458, 1614, 1633, 4916
HphI GGTGA 7 cut(s) 221, 778, 2893, 3512, 3761, 3778, 4859
Hpy166II GTNNAC 5 cut(s) 602, 2212, 2331, 3817, 4010
Hpy8I GTNNAC 5 cut(s) 602, 2212, 2331, 3817, 4010
Hpy99I CGWCG 1 cut(s) 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 170, 1210, 1946, 3383, 3448
KflI GGGWCCC 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 1632, 4915
KpnI GGTACC 2 cut(s) 2274, 4652
Ksp22I TGATCA 2 cut(s) 697, 2650
MaeIII GTNAC 7 cut(s) 985, 2413, 2881, 3806, 4418, 4847, 4903
MlsI TGGCCA 2 cut(s) 3650, 4801
MluNI TGGCCA 2 cut(s) 3650, 4801
MlyI GAGTC 5 cut(s) 330, 446, 2945, 4573, 4697
MmeI TCCRAC 3 cut(s) 703, 2124, 3945
Mox20I TGGCCA 2 cut(s) 3650, 4801
MroI TCCGGA 2 cut(s) 1632, 4915
MroXI GAANNNNTTC 3 cut(s) 23, 4068, 4789
MscI TGGCCA 2 cut(s) 3650, 4801
MslI CAYNNNNRTG 7 cut(s) 270, 1700, 3053, 3071, 3168, 4059, 4503
Msp20I TGGCCA 2 cut(s) 3650, 4801
MspA1I CMGCKG 1 cut(s) 3992
MspCI CTTAAG 1 cut(s) 2090
MspI CCGG 5 cut(s) 207, 458, 1614, 1633, 4916
MspR9I CCNGG 8 cut(s) 200, 262, 1320, 1614, 2627, 3684, 3711, 4619
Mva1269I GAATGC 2 cut(s) 2380, 3121
MvaI CCWGG 7 cut(s) 200, 262, 1320, 2627, 3684, 3711, 4619
MvnI CGCG 1 cut(s) 2233
MwoI GCNNNNNNNGC 6 cut(s) 77, 170, 1210, 1946, 3383, 3448
NciI CCSGG 1 cut(s) 1614
NcoI CCATGG 2 cut(s) 3066, 3437
NlaIV GGNNCC 9 cut(s) 15, 55, 56, 2272, 3009, 3867, 4593, 4650, 4761
NmuCI GTSAC 6 cut(s) 985, 2413, 2881, 4418, 4847, 4903
NruI TCGCGA 1 cut(s) 2233
NspI RCATGY 2 cut(s) 515, 3394
NspV TTCGAA 2 cut(s) 690, 1763
OliI CACNNNNGTG 1 cut(s) 4503
PaeR7I CTCGAG 1 cut(s) 2508
PagI TCATGA 3 cut(s) 2017, 3574, 4080
PceI AGGCCT 3 cut(s) 145, 2753, 3591
PciI ACATGT 1 cut(s) 511
PctI GAATGC 2 cut(s) 2380, 3121
PdmI GAANNNNTTC 3 cut(s) 23, 4068, 4789
PflMI CCANNNNNTGG 1 cut(s) 3472
PfoI TCCNGGA 2 cut(s) 198, 2625
PleI GAGTC 5 cut(s) 330, 445, 2944, 4573, 4696
PmaCI CACGTG 1 cut(s) 1966
PmlI CACGTG 1 cut(s) 1966
PpsI GAGTC 5 cut(s) 330, 445, 2944, 4573, 4696
Ppu21I YACGTR 1 cut(s) 1966
PpuMI RGGWCCY 3 cut(s) 54, 4591, 4924
PscI ACATGT 1 cut(s) 511
PshBI ATTAAT 2 cut(s) 1221, 4029
PsiI TTATAA 2 cut(s) 63, 2168
Psp124BI GAGCTC 2 cut(s) 754, 4371
Psp1406I AACGTT 2 cut(s) 871, 4066
Psp5II RGGWCCY 3 cut(s) 54, 4591, 4924
Psp6I CCWGG 7 cut(s) 198, 260, 1318, 2625, 3682, 3709, 4617
PspCI CACGTG 1 cut(s) 1966
PspFI CCCAGC 1 cut(s) 2095
PspGI CCWGG 7 cut(s) 198, 260, 1318, 2625, 3682, 3709, 4617
PspN4I GGNNCC 9 cut(s) 15, 55, 56, 2272, 3009, 3867, 4593, 4650, 4761
PspPI GGNCC 7 cut(s) 54, 171, 3368, 4269, 4591, 4615, 4924
PspPPI RGGWCCY 3 cut(s) 54, 4591, 4924
PspXI VCTCGAGB 1 cut(s) 2508
PstI CTGCAG 2 cut(s) 436, 4549
PstNI CAGNNNCTG 3 cut(s) 737, 1658, 4907
RruI TCGCGA 1 cut(s) 2233
RseI CAYNNNNRTG 7 cut(s) 270, 1700, 3053, 3071, 3168, 4059, 4503
SacI GAGCTC 2 cut(s) 754, 4371
Sau96I GGNCC 7 cut(s) 54, 171, 3368, 4269, 4591, 4615, 4924
ScaI AGTACT 2 cut(s) 3619, 3985
SchI GAGTC 5 cut(s) 330, 446, 2945, 4573, 4697
ScrFI CCNGG 8 cut(s) 200, 262, 1320, 1614, 2627, 3684, 3711, 4619
SfcI CTRYAG 5 cut(s) 432, 2111, 2755, 3310, 4545
Sfr274I CTCGAG 1 cut(s) 2508
SfuI TTCGAA 2 cut(s) 690, 1763
SinI GGWCC 4 cut(s) 54, 4591, 4615, 4924
SlaI CTCGAG 1 cut(s) 2508
SmiMI CAYNNNNRTG 7 cut(s) 270, 1700, 3053, 3071, 3168, 4059, 4503
SmlI CTYRAG 7 cut(s) 2067, 2090, 2508, 2694, 2911, 3488, 4182
SmoI CTYRAG 7 cut(s) 2067, 2090, 2508, 2694, 2911, 3488, 4182
SpeI ACTAGT 1 cut(s) 1787
SseBI AGGCCT 3 cut(s) 145, 2753, 3591
SspI AATATT 4 cut(s) 1360, 2437, 3481, 4033
SstI GAGCTC 2 cut(s) 754, 4371
StuI AGGCCT 3 cut(s) 145, 2753, 3591
StyD4I CCNGG 8 cut(s) 198, 260, 1318, 1612, 2625, 3682, 3709, 4617
StyI CCWWGG 5 cut(s) 1001, 2312, 3066, 3437, 4935
TatI WGTACW 5 cut(s) 1750, 2211, 3262, 3617, 3983
TseFI GTSAC 6 cut(s) 985, 2413, 2881, 4418, 4847, 4903
Tsp45I GTSAC 6 cut(s) 985, 2413, 2881, 4418, 4847, 4903
TspGWI ACGGA 6 cut(s) 348, 1474, 1493, 3261, 3523, 4273
Van91I CCANNNNNTGG 1 cut(s) 3472
Vha464I CTTAAG 1 cut(s) 2090
VneI GTGCAC 1 cut(s) 600
VpaK11BI GGWCC 4 cut(s) 54, 4591, 4615, 4924
VspI ATTAAT 2 cut(s) 1221, 4029
XagI CCTNNNNNAGG 1 cut(s) 3585
XbaI TCTAGA 1 cut(s) 2932
XceI RCATGY 2 cut(s) 515, 3394
XcmI CCANNNNNNNNNTGG 1 cut(s) 4122
XhoI CTCGAG 1 cut(s) 2508
XmaJI CCTAGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 2330
XmnI GAANNNNTTC 3 cut(s) 23, 4068, 4789
ZrmI AGTACT 2 cut(s) 3619, 3985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.