MD15G1427500.v1.1

UDP-glucose glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006844
Physical Location & Seq
Reverse (-)
52793419 .. 52816133
22715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1427500.v1.1.491

Sequence Viewer

Length: 4902 bp
ATGAGGACCCGTTTTAAATCTGCGTTTTGTGCTGTGATCGTCCTGGTTTGTTTGGGAGCGTCCGGAATCGGTTTAGTTTCGGGTCAGAATCGGAGGCCCAAGAATGTTCAGGCCGCGGTTCGGGCCAAGTGGTCGGGTACTCCATTGCTCCTGGAAGCCGGTGAATTACTATCTAAAGAACAAAAAGACCATTTTTGGGACTTCATTGATGCCTGGCATCATAGTGAGAAGGATGATGCTGAATCTTATACTGCTAAGGGCTGCCTAAAAAAAATTGTAAAGCACGGACTCTCTATTTTAAACGAACCATTGGCATCTTTGTTTGAATTCTCCCTCATGCTTAGATCTACATCTCCAAGATTGGTTCTTTATCGGCAATTAGCTGAGGAGGCTCTGTCTTCTTTTCCTCTGGTTGATGAAACTAATTCAAGCAGTGACAGTGGAATTTCTGAAACAAATGAGCTTATGGAAGGTAAAAGGTCAGATCTTTTGAACATTGGTCGAAACCCGAAGAGCCCTAATGGGAAATGTTGTTGGGTGGATACTGGAGGTGCACTGTTCTTTGATCCAGCAGACTTGAAAATTTGGCTTCAAAGTCCAAGAGATTCTTCTGGAGATTCATTTCAGCAGCCTGAACTTTTCGAATTTGATCACATCCATTTTGATTCAAGTGTTGGAAGTCCTGTTGCTGTTCTTTATGGAGCTCTTGGAACTGATTGCTTTAGGGAGTTTCATCTCACCCTAGTTGAAGCTGCAAAAGAGGGAAAAGCTAAGTATGTTGTTAGACAAGTATTACCTTCTGGCTGTGATGCCAAGATTGACCGTTGTGGTGCTGTTGGTACAAGAGATTCATTAAACTTGGGAGGCTATGGAGTAGAACTTGCTTTGAAGAATATGGAATATAAGGCTATGGATGATAGTGCTATAAAGAAAGGTGTCACATTAGAAGATCCCAGGATTGAAGATCTCAGCCAAGAAGTCAGAGGGTTTATATTCTCCAAAATTCTGGAACGCAAACCTGAACTCAGTTCAGAGATAATGGCATTCAGGGATTATCTTTTGTCATCAACAATATCTGACACACTTGATGTTTGGGAACTAAAAGATTTAGGACATCAAACTGCACAGAGGATAGTTCAAGCCTCCGATCCTTTGCAAGCAATGCAGGAAATTAATCAAAATTTTCCGAGCATCGTCTCTTCCTTATCTCGCATGAAGCTCAATGATTCAGTTAAAGATGAAATAAGTGCAAACCAGAGAATGATCCCACCTGGCAAGTCCTTAATGGCTCTGAATGGTGCTTTACTCAATATTGAAGATATTGACCTTTATCTGTTGCTTGATTTGGTTCATCAGGATTTATCGTTGGCTGATCAGTTTTCAAAGTTGAAGATTCCTCATGGCACCATACGGAAACTCCTGGCGAGTCTGCCTCCTCCAGAATCTAATATGCTCCGTGTAGATTTTCGTTCAGATCATGTTCAGTATCTGAATGACATAGAAGAAGATGATATGTATAGACGATGGAGGAGTAATTTAAATGAGATTTTGATGCCAGTCTTCCCCGGTCAGCTACGTTATATTCGCAAAAACCTTTTCCATGCAGTTTCTGTTATTGATCCATCAACTGTTTGTGGTCTTCAGTCCATTGACATGATTTTGTCTCTTTACGAGAACAATTTTCCTATGAGATTTGGAGTTGTACTGTATTCTTCGAAGTTCATCAAGCAGATTGAAACTAGGGGCAGTGAAGATGACCATGAAATTGAAGAGGATATGTCCAGCTTGATTATACGTCTCTTCATTTATATAAAGGAGAATCATGGAATTCAAACAGCTTTCCAGTTTTTGAGCAATATAAACAAATTGCGGATTGATTCTGAAGATTCTGCCGATGATGCTCTTGAAATGCACCATGTGGAAGGAGCATTTATAGAAACTGTATTGCCCAATACAAAATCTCCACCTCAAGATTTATTGCTAAAGCTGGAGAAGGAGCAAACTTTCAAGGAGCTTTCGCAAGAAAGCTCCATGTTTGTCTTTAAGCTGGGTTTGGCTAAGCTCCAGTGTTGCCTGTTGATGAATGGCCTTGTGTTGGAGTCTAATGAGGAGGCTCTTATAAATTCCATGAATGATGAGCTTCCCAGAATACAGGAGCAAGTGTACTATGGGCATATAAATTCCCGAACAGATGTTCTTGACAAGTTTCTGTCAGAAAGTAGTACTACTCGCTATAATCCACAGATTATTGCTGGTGGAAAGCCAAGGTTCATTTCTCTGCCTACATCTATTCTTGGAGGGGATGGTGTCTTAAATGATATCAACTATTTGCATTCTCCCGAAACTATGGATGATTTGAAGCCTGTGACCCATCTTCTAGCTGTTAATATCGCATCAAAGAAAGGGATGAAGCTGCTTCATGAAGGCTTACAATATCTGATAGAAGCGTCCAATCGCGCTCGAGTAGGTGTGCTTTTCAGTGTGAATCAGGATGCTGATGTCTCTAGTCATCTTTTTGTGAAGGTGTTTGAAATCACTGCATCCTCGTATAGCCATAAGAAAAAGGTGTTAGATTTCCTGGATCAGATGTGCTCATTCTATGAACATAATTATTTGCTTGCATCATCTAAGGGTACCGAAAGCACTCAAGAATTTATAGATAAAGTGTGCGAATTGGCTGAGGCTAATGGTTTATCATCAAAGGCCTACAGATTTTCCCTTTCTGAATTTTCTGATGAGAATTTGAGAAAATCTATGAATAAGGTATCCCAATTTTTGTATAGGCAGCTTGGCCTTGAATCTGGTGTTAATGCAGTCATTACTAATGGAAGGGTGACAGTTGTAAATGATGGTGGCACATTCTTGAGCCATGATTTACGCCTTCTAGAGTCTCTAGAGTTTGCCCAGAGAATAAAGCACATTGTGGAAATTATTGAGGAAGTGAAGTGGGAGGATATGGATCCCGACATCCTAACAAGCAAATTCATTAGTGATACTATAATGTCTGTGTCATCTTCCATGGCTATGCGGGATCGAAGTTCTGAAAGTGCCCGCTTTGAAGTTTTGAGTGCACAATATAGTGCCATTGTTTTGAACAATGAAAATGCTAGCATTCATATCGATGCAGTTATTGATCCCTTAAGTCCATTTGGGCAAAAGCTATCGTCAATTCTTCGAGTCCTGTGGAAATACACTCAGCCTAGCATGAGGATTGTACTAAATCCTTTGAGTTCTCTTGTTGATCTTCCTCTGAAGAATTACTACAGATACGTCCTACCAACAGTGGATGACTTCAGCAGCACTGACTACACAATAAATGGGCCAAAAGCCTTTTTTGCAAACATGCCACTGTCCAAAACACTCACAATGAATCTTGATGTTCCTGATCCATGGCTAGTTGAGCCTGTTATTGCAGTCCATGACTTGGATAATATTTTGCTTGAGAATCTTGGTGAGACACGAACATTACAAGCCGTGTTTGAACTTGAAGCTCTTGTTCTTACGGGTCATTGTTCTGAGAAAGGTCATGATCACCCCAGAGGCCTTCAGTTGATTATTGGTACTAAGAGTACTCCTCACTTGGTTGATACTCTTGTGATGGCCAATCTGGGTTATTGGCAAATGAAAGTGTCTCCTGGCGTTTGGTACTTGCAACTTGCTCCAGGTAGAAGTTCTGAACTTTATTTTCTGAAAGAGGATGGCAACGAAAGTGGGAGCAAAACATTATCCAAACGTATCACCATAGACGATTTACGGGGTAAAGTAGTTCACATGGAAGTAGCAAAGAAAAAGGGAAAGGAGCATGAGAAATTGTTGGTTCCTGATGGCGAAGACAACTCACACGATGATAAGGAGGGAAGTAGCTGGAACTCCAACTTCTTGAAATGGGCTTCTGGTTTTATTGGTGGTGGTGAGCAATCTAAAAAGAGTGAAAGTACTTCAGCGGAGCAAGGAAAGGGTGGACGACATGGAAAAACGATTAATATTTTTTCTATTGCTTCTGGACACCTATATGAACGTTTTCTGAAAATCATGATTTTAAGTGTCTTGAAGAATACCCATCGTCCGGTCAAATTCTGGTTCATAAAGAACTATCTTTCTCCTCAGTTCAAGGATGTCATTCCACCTATGGCTCAAGAATATGGTTTTGAGTATGAACTGATCACCTACAAGTGGCCTACATGGTTGCACAAGCAAAAAGAAAAGCAACGGATTATTTGGGCCTATAAAATCTTGTTCCTTGATGTTATCTTTCCCCTTTCTTTGGAAAAGGTCATATTTGTTGACGCAGATCAAATTGTTAGGGCAGACATGGGAGAGCTGTATGACATGGATATAAAAGGACGACCTCTTGCATATACTCCATTTTGTGACAACAACAAGGATATGGATGGATATCGGTTTTGGAGACAAGGTTTCTGGAAAGAACATTTACGTGGGAGATCTTACCACATAAGTGCTTTATATGTTGTCGATTTGAAGAAATTTCGAGAGACTGCAGCAGGAGATAATCTGAGAGTTTTCTATGAGACTCTTAGCAAGGACCCAAACAGTCTATCTAATTTAGACCAGGATCTTCCAAACTATGCTCAACATACAGTACCCATCTTTTCATTACCGCAAGAATGGCTGTGGTGCGAGTCATGGTGCGGTAATGCAACTAAATCCAAGGCAAAAACCATTGATCTTTGCAACAATCCCATGACAAAGGAACCCAAGCTTCAGGGCGCTAGAAGAATAGTTTCTGAATGGCCAGATCTTGATTTGGAGGCAAGGCAATTCACTGCAAAGATCTTAGGTGACGAACTGGACATCCAAGAGCCTGCACCACTTCCTAATGAACCAGAGAAATCAGTGACCGGCAGTCCGGAGGAGGATCTTGAATCCAAGGCAGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003980 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006011 GO:0006139 GO:0006457 GO:0006458 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006515 GO:0006725 GO:0006793 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008194 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009626 GO:0009719 GO:0009725 GO:0009751 GO:0009812 GO:0009987 GO:0010033 GO:0010204 GO:0010243 GO:0010498 GO:0012501 GO:0012505 GO:0014070 GO:0016740 GO:0016757 GO:0016758 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0023052 GO:0030163 GO:0030968 GO:0031347 GO:0031349 GO:0033554 GO:0034050 GO:0034620 GO:0034641 GO:0034645 GO:0034976 GO:0035251 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042221 GO:0042440 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046283 GO:0046483 GO:0046527 GO:0046677 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051084 GO:0051603 GO:0051716 GO:0051788 GO:0055086 GO:0065007 GO:0070085 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0080134 GO:0097359 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Glyco_transf_8 UDP-g_GGTase

Protein Analysis

1634

Amino Acids

184.54

Weight (kDa)

5.46

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_12 PF18400 47 - 271 2.4e-50 Thioredoxin-like domain
Thioredoxin_13 PF18401 359 - 475 4.7e-34 Thioredoxin-like domain
Thioredoxin_14 PF18402 488 - 749 3.1e-58 Thioredoxin-like domain
Thioredoxin_15 PF18403 770 - 1008 2.8e-40 Thioredoxin-like domain
UDP-g_GGTase PF06427 1169 - 1272 6e-29 UDP-glucose:Glycoprotein Glucosyltransferase
Glyco_transf_24 PF18404 1327 - 1593 7.5e-144 Glucosyltransferase 24
Glyco_transf_8 PF01501 1338 - 1558 2.3e-07 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2120
Acc65I GGTACC 1 cut(s) 2633
AccB1I GGYRCC 2 cut(s) 1403, 2633
AccB7I CCANNNNNTGG 1 cut(s) 3424
AccII CGCG 2 cut(s) 116, 2457
AccIII TCCGGA 2 cut(s) 62, 4867
AciI CCGC 8 cut(s) 114, 116, 1870, 3028, 3052, 3944, 4619, 4650
AclI AACGTT 1 cut(s) 4018
AcoI YGGCCR 2 cut(s) 3600, 4751
AcuI CTGAAG 7 cut(s) 1625, 1902, 3272, 3277, 3530, 3924, 4706
AdeI CACNNNGTG 2 cut(s) 1918, 2923
AfiI CCNNNNNNNGG 7 cut(s) 120, 196, 2095, 3424, 4066, 4173, 4264
AflII CTTAAG 1 cut(s) 3139
AhdI GACNNNNNGTC 1 cut(s) 4863
AjuI GAANNNNNNNTTGG 4 cut(s) 592, 624, 2257, 2289
AleI CACNNNNGTG 1 cut(s) 4455
AloI GAACNNNNNNTCC 2 cut(s) 4797, 4829
Alw21I GWGCWC 4 cut(s) 556, 706, 2594, 3073
Alw44I GTGCAC 2 cut(s) 552, 3069
AlwNI CAGNNNCTG 2 cut(s) 1489, 1610
Ama87I CYCGRG 1 cut(s) 2460
Aor13HI TCCGGA 2 cut(s) 62, 4867
ApaLI GTGCAC 2 cut(s) 552, 3069
ApeKI GCWGC 7 cut(s) 261, 628, 752, 2413, 2785, 3297, 4499
AseI ATTAAT 2 cut(s) 1173, 3981
Asp700I GAANNNNTTC 2 cut(s) 4020, 4741
Asp718I GGTACC 1 cut(s) 2633
AspLEI GCGC 2 cut(s) 2459, 4730
AspS9I GGNCC 6 cut(s) 6, 96, 123, 3320, 4221, 4543
AsuC2I CCSGG 1 cut(s) 1566
AsuHPI GGTGA 9 cut(s) 173, 730, 2845, 3464, 3524, 3730, 3923, 4156, 4811
AsuII TTCGAA 2 cut(s) 642, 1715
AsuNHI GCTAGC 1 cut(s) 3107
AvaI CYCGRG 1 cut(s) 2460
AvaII GGWCC 2 cut(s) 6, 4543
BaeGI GKGCMC 3 cut(s) 556, 3052, 3073
BaeI ACNNNNGTAYC 2 cut(s) 3579, 3612
BalI TGGCCA 2 cut(s) 3602, 4753
BamHI GGATCC 1 cut(s) 2959
BanI GGYRCC 2 cut(s) 1403, 2633
BanII GRGCYC 2 cut(s) 518, 706
BarI GAAGNNNNNNTAC 2 cut(s) 3245, 3277
BbsI GAAGAC 4 cut(s) 390, 1552, 1631, 3837
Bbv12I GWGCWC 4 cut(s) 556, 706, 2594, 3073
BbvCI CCTCAGC 2 cut(s) 384, 2679
BbvI GCAGC 7 cut(s) 248, 640, 739, 2400, 2797, 3309, 4511
BceAI ACGGC 1 cut(s) 3458
BcgI CGANNNNNNTGC 2 cut(s) 3100, 3134
BciVI GTATCC 2 cut(s) 535, 2776
BclI TGATCA 4 cut(s) 649, 1372, 3529, 4161
BcnI CCSGG 1 cut(s) 1566
BfmI CTRYAG 3 cut(s) 2707, 3262, 4497
BfoI RGCGCY 1 cut(s) 4731
BfrI CTTAAG 1 cut(s) 3139
BfuI GTATCC 2 cut(s) 535, 2776
BglII AGATCT 6 cut(s) 344, 484, 964, 4442, 4756, 4791
BisI GCNGC 8 cut(s) 114, 262, 629, 753, 2414, 2786, 3298, 4500
BlpI GCTNAGC 1 cut(s) 2058
BlsI GCNGC 8 cut(s) 115, 263, 630, 754, 2415, 2787, 3299, 4501
BmcAI AGTACT 3 cut(s) 2224, 3571, 3937
Bme18I GGWCC 2 cut(s) 6, 4543
BmeRI GACNNNNNGTC 1 cut(s) 4863
BmeT110I CYCGRG 1 cut(s) 2460
BmgT120I GGNCC 6 cut(s) 6, 96, 123, 3320, 4221, 4543
BmiI GGNNCC 7 cut(s) 8, 1405, 2635, 2961, 3819, 4545, 4713
BmtI GCTAGC 1 cut(s) 3111
BpiI GAAGAC 4 cut(s) 390, 1552, 1631, 3837
BplI GAGNNNNNCTC 4 cut(s) 1417, 1449, 3559, 3591
BpmI CTGGAG 6 cut(s) 567, 633, 1422, 2009, 2048, 3645
Bpu10I CCTNAGC 3 cut(s) 255, 384, 2679
Bpu1102I GCTNAGC 1 cut(s) 2058
Bpu14I TTCGAA 2 cut(s) 642, 1715
BpuEI CTTGAG 5 cut(s) 1953, 2631, 2884, 3461, 4119
BpuMI CCSGG 1 cut(s) 1566
Bsa29I ATCGAT 1 cut(s) 3120
BsaAI YACGTR 1 cut(s) 4436
BsaBI GATNNNNATC 3 cut(s) 349, 2958, 4395
BsaJI CCNNGG 8 cut(s) 114, 953, 1564, 2264, 3018, 3389, 4668, 4887
BsaWI WCCGGW 3 cut(s) 62, 4066, 4867
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 196, 2095, 3424, 4066, 4173, 4264
Bse118I RCCGGY 2 cut(s) 158, 4859
Bse1I ACTGG 5 cut(s) 550, 1556, 1843, 2065, 4812
Bse3DI GCAATG 2 cut(s) 143, 1167
Bse8I GATNNNNATC 3 cut(s) 349, 2958, 4395
BseAI TCCGGA 2 cut(s) 62, 4867
BseCI ATCGAT 1 cut(s) 3120
BseDI CCNNGG 8 cut(s) 114, 953, 1564, 2264, 3018, 3389, 4668, 4887
BseJI GATNNNNATC 3 cut(s) 349, 2958, 4395
BseLI CCNNNNNNNGG 7 cut(s) 120, 196, 2095, 3424, 4066, 4173, 4264
BseMI GCAATG 2 cut(s) 143, 1167
BseMII CTCAG 8 cut(s) 375, 982, 1039, 2670, 3209, 3507, 4118, 4505
BseNI ACTGG 5 cut(s) 550, 1556, 1843, 2065, 4812
BseRI GAGGAG 7 cut(s) 401, 1425, 1543, 2123, 3564, 4092, 4886
BseSI GKGCMC 3 cut(s) 556, 3052, 3073
BseXI GCAGC 7 cut(s) 248, 640, 739, 2400, 2797, 3309, 4511
BseYI CCCAGC 1 cut(s) 2047
BsgI GTGCAG 2 cut(s) 1107, 4809
Bsh1236I CGCG 2 cut(s) 116, 2457
BshNI GGYRCC 2 cut(s) 1403, 2633
BshVI ATCGAT 1 cut(s) 3120
BsiHKAI GWGCWC 4 cut(s) 556, 706, 2594, 3073
BsiHKCI CYCGRG 1 cut(s) 2460
BsiSI CCGG 6 cut(s) 63, 159, 1566, 4067, 4860, 4868
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 7 cut(s) 120, 196, 2095, 3424, 4066, 4173, 4264
BsmBI CGTCTC 2 cut(s) 1201, 1802
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 3 cut(s) 1043, 2332, 3111
BsoBI CYCGRG 1 cut(s) 2460
Bsp119I TTCGAA 2 cut(s) 642, 1715
Bsp1286I GDGCHC 6 cut(s) 518, 556, 706, 2594, 3052, 3073
Bsp13I TCCGGA 2 cut(s) 62, 4867
Bsp1720I GCTNAGC 1 cut(s) 2058
Bsp19I CCATGG 2 cut(s) 3018, 3389
BspACI CCGC 8 cut(s) 114, 116, 1870, 3028, 3052, 3944, 4619, 4650
BspCNI CTCAG 8 cut(s) 376, 981, 1038, 2671, 3208, 3508, 4117, 4506
BspDI ATCGAT 1 cut(s) 3120
BspEI TCCGGA 2 cut(s) 62, 4867
BspFNI CGCG 2 cut(s) 116, 2457
BspHI TCATGA 3 cut(s) 2419, 3526, 4032
BspLI GGNNCC 7 cut(s) 8, 1405, 2635, 2961, 3819, 4545, 4713
BspMAI CTGCAG 1 cut(s) 4501
BspOI GCTAGC 1 cut(s) 3111
BspQI GCTCTTC 1 cut(s) 506
BspT104I TTCGAA 2 cut(s) 642, 1715
BspT107I GGYRCC 2 cut(s) 1403, 2633
BspTI CTTAAG 1 cut(s) 3139
BsrDI GCAATG 2 cut(s) 143, 1167
BsrFI RCCGGY 2 cut(s) 158, 4859
BsrI ACTGG 5 cut(s) 550, 1556, 1843, 2065, 4812
BssAI RCCGGY 2 cut(s) 158, 4859
BssECI CCNNGG 8 cut(s) 114, 953, 1564, 2264, 3018, 3389, 4668, 4887
BssT1I CCWWGG 5 cut(s) 2264, 3018, 3389, 4668, 4887
Bst6I CTCTTC 4 cut(s) 506, 1204, 1764, 1805
BstAFI CTTAAG 1 cut(s) 3139
BstAPI GCANNNNNTGC 1 cut(s) 1162
BstBAI YACGTR 1 cut(s) 4436
BstBI TTCGAA 2 cut(s) 642, 1715
BstC8I GCNNGC 5 cut(s) 1158, 2619, 3052, 3109, 4824
BstDSI CCRYGG 3 cut(s) 114, 3018, 3389
BstFNI CGCG 2 cut(s) 116, 2457
BstH2I RGCGCY 1 cut(s) 4731
BstHHI GCGC 2 cut(s) 2459, 4730
BstMWI GCNNNNNNNGC 8 cut(s) 29, 122, 389, 1162, 1898, 3335, 3400, 4627
BstNSI RCATGY 1 cut(s) 3346
BstSFI CTRYAG 3 cut(s) 2707, 3262, 4497
BstSLI GKGCMC 3 cut(s) 556, 3052, 3073
BstUI CGCG 2 cut(s) 116, 2457
BstV1I GCAGC 7 cut(s) 248, 640, 739, 2400, 2797, 3309, 4511
BstV2I GAAGAC 4 cut(s) 390, 1552, 1631, 3837
BstXI CCANNNNNNTGG 1 cut(s) 1006
Bsu15I ATCGAT 1 cut(s) 3120
BsuI GTATCC 2 cut(s) 535, 2776
BsuTUI ATCGAT 1 cut(s) 3120
BtgI CCRYGG 3 cut(s) 114, 3018, 3389
BtsI GCAGTG 4 cut(s) 439, 1753, 2535, 4782
Cac8I GCNNGC 5 cut(s) 1158, 2619, 3052, 3109, 4824
CaiI CAGNNNCTG 2 cut(s) 1489, 1610
CciI TCATGA 3 cut(s) 2419, 3526, 4032
CfoI GCGC 2 cut(s) 2459, 4730
Cfr10I RCCGGY 2 cut(s) 158, 4859
Cfr13I GGNCC 6 cut(s) 6, 96, 123, 3320, 4221, 4543
Cfr42I CCGCGG 1 cut(s) 117
ClaI ATCGAT 1 cut(s) 3120
CseI GACGC 3 cut(s) 48, 2436, 4295
CspCI CAANNNNNGTGG 2 cut(s) 3691, 3726
DraI TTTAAA 3 cut(s) 16, 300, 1539
DraIII CACNNNGTG 2 cut(s) 1918, 2923
DriI GACNNNNNGTC 1 cut(s) 4863
EaeI YGGCCR 2 cut(s) 3600, 4751
Eam1104I CTCTTC 4 cut(s) 506, 1204, 1764, 1805
Eam1105I GACNNNNNGTC 1 cut(s) 4863
EarI CTCTTC 4 cut(s) 506, 1204, 1764, 1805
Ecl136II GAGCTC 1 cut(s) 704
Eco130I CCWWGG 5 cut(s) 2264, 3018, 3389, 4668, 4887
Eco147I AGGCCT 2 cut(s) 2705, 3543
Eco24I GRGCYC 2 cut(s) 518, 706
Eco32I GATATC 2 cut(s) 2320, 4397
Eco47I GGWCC 2 cut(s) 6, 4543
Eco53kI GAGCTC 1 cut(s) 704
Eco57I CTGAAG 7 cut(s) 1625, 1902, 3272, 3277, 3530, 3924, 4706
Eco88I CYCGRG 1 cut(s) 2460
EcoICRI GAGCTC 1 cut(s) 704
EcoO109I RGGNCCY 2 cut(s) 6, 4543
EcoRI GAATTC 2 cut(s) 326, 1827
EcoRV GATATC 2 cut(s) 2320, 4397
EcoT14I CCWWGG 5 cut(s) 2264, 3018, 3389, 4668, 4887
EcoT38I GRGCYC 2 cut(s) 518, 706
ErhI CCWWGG 5 cut(s) 2264, 3018, 3389, 4668, 4887
Esp3I CGTCTC 2 cut(s) 1201, 1802
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 212
FauI CCCGC 2 cut(s) 3021, 3059
FbaI TGATCA 4 cut(s) 649, 1372, 3529, 4161
Fnu4HI GCNGC 8 cut(s) 114, 262, 629, 753, 2414, 2786, 3298, 4500
FriOI GRGCYC 2 cut(s) 518, 706
Fsp4HI GCNGC 8 cut(s) 114, 262, 629, 753, 2414, 2786, 3298, 4500
GlaI GCGC 2 cut(s) 2458, 4729
GluI GCNGC 8 cut(s) 114, 262, 629, 753, 2414, 2786, 3298, 4500
GsaI CCCAGC 1 cut(s) 2051
GsuI CTGGAG 6 cut(s) 567, 633, 1422, 2009, 2048, 3645
HaeII RGCGCY 1 cut(s) 4731
HapII CCGG 6 cut(s) 63, 159, 1566, 4067, 4860, 4868
HgaI GACGC 3 cut(s) 48, 2436, 4295
HhaI GCGC 2 cut(s) 2459, 4730
Hin6I GCGC 2 cut(s) 2457, 4728
HinP1I GCGC 2 cut(s) 2457, 4728
HincII GTYRAC 1 cut(s) 4285
HindII GTYRAC 1 cut(s) 4285
HindIII AAGCTT 1 cut(s) 4718
HpaII CCGG 6 cut(s) 63, 159, 1566, 4067, 4860, 4868
HphI GGTGA 9 cut(s) 173, 730, 2845, 3464, 3524, 3730, 3923, 4156, 4811
Hpy166II GTNNAC 6 cut(s) 554, 2164, 3071, 3769, 3962, 4285
Hpy8I GTNNAC 6 cut(s) 554, 2164, 3071, 3769, 3962, 4285
HpyCH4IV ACGT 6 cut(s) 1576, 1795, 3270, 3733, 4018, 4435
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 122, 389, 1162, 1898, 3335, 3400, 4627
HpySE526I ACGT 6 cut(s) 1576, 1795, 3270, 3733, 4018, 4435
HspAI GCGC 2 cut(s) 2457, 4728
Kpn2I TCCGGA 2 cut(s) 62, 4867
KpnI GGTACC 1 cut(s) 2637
Ksp22I TGATCA 4 cut(s) 649, 1372, 3529, 4161
KspI CCGCGG 1 cut(s) 117
LguI GCTCTTC 1 cut(s) 506
Lsp1109I GCAGC 7 cut(s) 248, 640, 739, 2400, 2797, 3309, 4511
MaeII ACGT 6 cut(s) 1576, 1795, 3270, 3733, 4018, 4435
MaeIII GTNAC 7 cut(s) 434, 937, 2365, 2833, 4370, 4799, 4855
MhlI GDGCHC 6 cut(s) 518, 556, 706, 2594, 3052, 3073
MlsI TGGCCA 2 cut(s) 3602, 4753
MluNI TGGCCA 2 cut(s) 3602, 4753
MlyI GAGTC 7 cut(s) 282, 1435, 2108, 2897, 3186, 4525, 4649
MmeI TCCRAC 3 cut(s) 655, 2076, 3897
Mox20I TGGCCA 2 cut(s) 3602, 4753
MroI TCCGGA 2 cut(s) 62, 4867
MroXI GAANNNNTTC 2 cut(s) 4020, 4741
MscI TGGCCA 2 cut(s) 3602, 4753
MslI CAYNNNNRTG 7 cut(s) 222, 1652, 3023, 3120, 4011, 4434, 4455
Msp20I TGGCCA 2 cut(s) 3602, 4753
MspA1I CMGCKG 2 cut(s) 116, 3944
MspCI CTTAAG 1 cut(s) 3139
MspI CCGG 6 cut(s) 63, 159, 1566, 4067, 4860, 4868
Mva1269I GAATGC 3 cut(s) 1043, 2332, 3111
MvnI CGCG 2 cut(s) 116, 2457
MwoI GCNNNNNNNGC 8 cut(s) 29, 122, 389, 1162, 1898, 3335, 3400, 4627
NciI CCSGG 1 cut(s) 1566
NcoI CCATGG 2 cut(s) 3018, 3389
NheI GCTAGC 1 cut(s) 3107
NlaIV GGNNCC 7 cut(s) 8, 1405, 2635, 2961, 3819, 4545, 4713
NmuCI GTSAC 7 cut(s) 434, 937, 2365, 2833, 4370, 4799, 4855
NspI RCATGY 1 cut(s) 3346
NspV TTCGAA 2 cut(s) 642, 1715
OliI CACNNNNGTG 1 cut(s) 4455
PaeR7I CTCGAG 1 cut(s) 2460
PagI TCATGA 3 cut(s) 2419, 3526, 4032
PceI AGGCCT 2 cut(s) 2705, 3543
PciSI GCTCTTC 1 cut(s) 506
PctI GAATGC 3 cut(s) 1043, 2332, 3111
PdmI GAANNNNTTC 2 cut(s) 4020, 4741
PflMI CCANNNNNTGG 1 cut(s) 3424
PfoI TCCNGGA 2 cut(s) 150, 2577
PkrI GCNGC 8 cut(s) 115, 263, 630, 754, 2415, 2787, 3299, 4501
PleI GAGTC 7 cut(s) 282, 1434, 2107, 2896, 3185, 4525, 4648
PpsI GAGTC 7 cut(s) 282, 1434, 2107, 2896, 3185, 4525, 4648
Ppu21I YACGTR 1 cut(s) 4436
PpuMI RGGWCCY 2 cut(s) 6, 4543
PshBI ATTAAT 2 cut(s) 1173, 3981
PsiI TTATAA 1 cut(s) 2120
Psp124BI GAGCTC 1 cut(s) 706
Psp1406I AACGTT 1 cut(s) 4018
Psp5II RGGWCCY 2 cut(s) 6, 4543
PspFI CCCAGC 1 cut(s) 2047
PspN4I GGNNCC 7 cut(s) 8, 1405, 2635, 2961, 3819, 4545, 4713
PspPI GGNCC 6 cut(s) 6, 96, 123, 3320, 4221, 4543
PspPPI RGGWCCY 2 cut(s) 6, 4543
PspXI VCTCGAGB 1 cut(s) 2460
PstI CTGCAG 1 cut(s) 4501
PstNI CAGNNNCTG 2 cut(s) 1489, 1610
RseI CAYNNNNRTG 7 cut(s) 222, 1652, 3023, 3120, 4011, 4434, 4455
SacI GAGCTC 1 cut(s) 706
SacII CCGCGG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 506
SatI GCNGC 8 cut(s) 114, 262, 629, 753, 2414, 2786, 3298, 4500
Sau96I GGNCC 6 cut(s) 6, 96, 123, 3320, 4221, 4543
ScaI AGTACT 3 cut(s) 2224, 3571, 3937
SchI GAGTC 7 cut(s) 282, 1435, 2108, 2897, 3186, 4525, 4649
SduI GDGCHC 6 cut(s) 518, 556, 706, 2594, 3052, 3073
SfcI CTRYAG 3 cut(s) 2707, 3262, 4497
Sfr274I CTCGAG 1 cut(s) 2460
Sfr303I CCGCGG 1 cut(s) 117
SfuI TTCGAA 2 cut(s) 642, 1715
SgrBI CCGCGG 1 cut(s) 117
SinI GGWCC 2 cut(s) 6, 4543
SlaI CTCGAG 1 cut(s) 2460
SmiI ATTTAAAT 1 cut(s) 1539
SmiMI CAYNNNNRTG 7 cut(s) 222, 1652, 3023, 3120, 4011, 4434, 4455
SmlI CTYRAG 7 cut(s) 1968, 2460, 2646, 2863, 3139, 3440, 4134
SmoI CTYRAG 7 cut(s) 1968, 2460, 2646, 2863, 3139, 3440, 4134
SseBI AGGCCT 2 cut(s) 2705, 3543
SsiI CCGC 8 cut(s) 114, 116, 1870, 3028, 3052, 3944, 4619, 4650
SspI AATATT 3 cut(s) 1312, 3433, 3985
SstI GAGCTC 1 cut(s) 706
StuI AGGCCT 2 cut(s) 2705, 3543
StyI CCWWGG 5 cut(s) 2264, 3018, 3389, 4668, 4887
SwaI ATTTAAAT 1 cut(s) 1539
TaiI ACGT 6 cut(s) 1579, 1798, 3273, 3736, 4021, 4438
TaqI TCGA 9 cut(s) 502, 642, 1715, 2461, 3034, 3120, 3175, 4473, 4489
TatI WGTACW 6 cut(s) 1702, 2163, 2222, 3214, 3569, 3935
TauI GCSGC 1 cut(s) 116
TseFI GTSAC 7 cut(s) 434, 937, 2365, 2833, 4370, 4799, 4855
TseI GCWGC 7 cut(s) 261, 628, 752, 2413, 2785, 3297, 4499
Tsp45I GTSAC 7 cut(s) 434, 937, 2365, 2833, 4370, 4799, 4855
TspGWI ACGGA 4 cut(s) 300, 1426, 1445, 4225
Van91I CCANNNNNTGG 1 cut(s) 3424
Vha464I CTTAAG 1 cut(s) 3139
VneI GTGCAC 2 cut(s) 552, 3069
VpaK11BI GGWCC 2 cut(s) 6, 4543
VspI ATTAAT 2 cut(s) 1173, 3981
XbaI TCTAGA 2 cut(s) 2884, 2893
XceI RCATGY 1 cut(s) 3346
XhoI CTCGAG 1 cut(s) 2460
XmnI GAANNNNTTC 2 cut(s) 4020, 4741
ZrmI AGTACT 3 cut(s) 2224, 3571, 3937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.