FvH4_5g29120

Aminomethyltransferase folate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005067
Physical Location & Seq
Reverse (-)
20197199 .. 20202081
4883 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29120.t1

Sequence Viewer

Length: 1251 bp
ATGGCAAAAGTAACATGCGAGTCTCTCCTTGTTAATCCATGGCGTATATTCGCTTATCCCCACCAAAGCTTTGCGGTTCCCTCGCTCACTCAGACTACTCATACCCAGAAGAAGCTCACACTCTCCTCAACCAGAACGCGAGCTTTGCCATTCGACCTCTCCCCTCCTCCTATTGACCACGACCTCCTGGACACTGTGGCAGCAGCAACTGCTGGAGCAACAGTTTCAGATGATGGGATTATCGAAACGTTCGACAACGATGACCAAGCTTTGGATGCTGCCGATAATGGTCTTGTGGTTGTGGACCTCTCGCATTTTTGCCGGATAAGAGTTAGTGGAGATGACAGGATCCAGTTTCTTCACAACCAAAGCACGGCCAATTTCGAATGTCTCCATGAAGGACAGGGATGTGACACAGTTTTTGTTACACCAACAGCTCGGACAATAGATATCGCACATGCATGGGTCATGAAAAATGCAGTAATGTTAATGGTTTCACCTGTTTCCAGAAGTATCTCTGAAATGCTGAAAAAGTACATATTTTTCAATGACAAGGTAGAGATTCAAGACATCACTAAGCAAACTTGCTTCTTCGTTTTAGTGGGACCTAAAAGCAACTATGTAATGGAGGAGTTAAACCTAGGTGATCTCGTTGGACAACCATACGGCACGCATCAGCATTTCAGTGTCAACGGGATGCCTGTTACTGTGGGAGTGGGAAATGTGATTTCTGAAAAAGGCTTTTCACTTTTGATGTCACCAGCTGCCGCTGGATCAGTCTGGAAAACTATTCTATCTCATGGTGCGACCCCAATGGGCTCTATTGCATGGGAAAAACTAAGAATTTTGCAAGGAAAGCCAGCTCCTCAGAAGGAGCTTACTAATGAATATAATGTCCTGGAGGCCGGTCTCTGGAATTCAATCTCTTTAACCAAAGGGTGCTATAAGGGACAGGAGACTATATCTCGACTGATAACGTACGATGGAGTCAAGCAAAGACTTTGGGGAATCTGTCTATCAGCACCAGCGGAACCTGGCAGCATCATTACAGTTGGTGGGAAAAAGGTTGGAAAGCTGACAAGTTGCACATCCGGAAGAAAGGAAAGTGAGTACTTTGGTTTAGGCTATATCAAGAGGCAAAATGTTTCAGAAGGAGACATGGTAGTGGTCGGAGATGCTATTACAGGTACAGTGGTAGAAGTACCTTTCCTTGCTGGGCAACGGCCACCATCGAGAAGCTCAAGCTCTTGA

Protein Analysis

417

Amino Acids

45.19

Weight (kDa)

6.21

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_T PF01571 91 - 312 4.9e-24 GCVT N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 271
AccII CGCG 1 cut(s) 139
AccIII TCCGGA 1 cut(s) 1091
AciI CCGC 3 cut(s) 74, 768, 1028
AclI AACGTT 1 cut(s) 248
AclWI GGATC 3 cut(s) 343, 356, 781
AcoI YGGCCR 2 cut(s) 375, 1223
AcsI RAATTY 2 cut(s) 843, 916
AfaI GTAC 5 cut(s) 536, 980, 1112, 1189, 1203
AfiI CCNNNNNNNGG 3 cut(s) 271, 373, 912
AgsI TTSAA 3 cut(s) 547, 566, 921
AjnI CCWGG 3 cut(s) 186, 897, 1033
Alw26I GTCTC 5 cut(s) 27, 395, 914, 950, 1149
AlwI GGATC 3 cut(s) 343, 356, 781
AlwNI CAGNNNCTG 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 1091
AoxI GGCC 3 cut(s) 375, 903, 1223
ApeKI GCWGC 5 cut(s) 200, 203, 278, 764, 1038
ApoI RAATTY 2 cut(s) 843, 916
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AspA2I CCTAGG 1 cut(s) 640
AspS9I GGNCC 2 cut(s) 304, 605
AsuHPI GGTGA 3 cut(s) 489, 656, 750
AsuII TTCGAA 1 cut(s) 384
AvaII GGWCC 2 cut(s) 304, 605
AvrII CCTAGG 1 cut(s) 640
BaeI ACNNNNGTAYC 2 cut(s) 1179, 1212
BamHI GGATCC 1 cut(s) 348
BanII GRGCYC 1 cut(s) 821
BbvI GCAGC 5 cut(s) 212, 215, 265, 751, 1050
BccI CCATC 3 cut(s) 227, 977, 1237
BceAI ACGGC 3 cut(s) 390, 682, 1238
BciT130I CCWGG 3 cut(s) 188, 899, 1035
BcoDI GTCTC 5 cut(s) 27, 395, 914, 950, 1149
BfaI CTAG 1 cut(s) 641
BisI GCNGC 6 cut(s) 201, 204, 279, 765, 768, 1039
BlnI CCTAGG 1 cut(s) 640
BlsI GCNGC 6 cut(s) 202, 205, 280, 766, 769, 1040
BmcAI AGTACT 1 cut(s) 1112
Bme1390I CCNGG 3 cut(s) 188, 899, 1035
Bme18I GGWCC 2 cut(s) 304, 605
BmgT120I GGNCC 2 cut(s) 304, 605
BmiI GGNNCC 4 cut(s) 78, 350, 606, 1032
BmrFI CCNGG 3 cut(s) 188, 899, 1035
BmsI GCATC 5 cut(s) 265, 682, 687, 1050, 1165
BpmI CTGGAG 2 cut(s) 234, 920
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 1225
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 38, 640
BsaWI WCCGGW 1 cut(s) 1091
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 1146, 1176
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 373, 912
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 1 cut(s) 352
BseAI TCCGGA 1 cut(s) 1091
BseBI CCWGG 3 cut(s) 188, 899, 1035
BseDI CCNNGG 2 cut(s) 38, 640
BseGI GGATG 4 cut(s) 280, 413, 702, 1088
BseLI CCNNNNNNNGG 3 cut(s) 271, 373, 912
BseMII CTCAG 2 cut(s) 104, 881
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 4 cut(s) 115, 156, 644, 855
BseXI GCAGC 5 cut(s) 212, 215, 265, 751, 1050
BseYI CCCAGC 1 cut(s) 1214
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 377, 905, 1225
BsiSI CCGG 3 cut(s) 322, 906, 1092
BsiWI CGTACG 1 cut(s) 978
BslFI GGGAC 2 cut(s) 618, 963
BslI CCNNNNNNNGG 3 cut(s) 271, 373, 912
BsmAI GTCTC 5 cut(s) 27, 395, 914, 950, 1149
BsmFI GGGAC 2 cut(s) 618, 963
BsnI GGCC 3 cut(s) 377, 905, 1225
Bso31I GGTCTC 1 cut(s) 914
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 821
Bsp13I TCCGGA 1 cut(s) 1091
Bsp143I GATC 3 cut(s) 348, 646, 773
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 3 cut(s) 74, 768, 1028
BspANI GGCC 3 cut(s) 377, 905, 1225
BspCNI CTCAG 2 cut(s) 103, 880
BspEI TCCGGA 1 cut(s) 1091
BspFNI CGCG 1 cut(s) 139
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 78, 350, 606, 1032
BspPI GGATC 3 cut(s) 343, 356, 781
BspT104I TTCGAA 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 914
BsrFI RCCGGY 1 cut(s) 905
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 2 cut(s) 38, 640
BssMI GATC 3 cut(s) 348, 646, 773
BssT1I CCWWGG 2 cut(s) 38, 640
Bst2UI CCWGG 3 cut(s) 188, 899, 1035
Bst4CI ACNGT 6 cut(s) 196, 223, 418, 709, 1051, 1192
BstAPI GCANNNNNTGC 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 141, 671, 861
BstDEI CTNAG 4 cut(s) 90, 576, 839, 867
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 280, 413, 702, 1088
BstFNI CGCG 1 cut(s) 139
BstKTI GATC 3 cut(s) 351, 649, 776
BstMAI GTCTC 5 cut(s) 27, 395, 914, 950, 1149
BstMBI GATC 3 cut(s) 348, 646, 773
BstMWI GCNNNNNNNGC 4 cut(s) 145, 209, 275, 856
BstNI CCWGG 3 cut(s) 188, 899, 1035
BstNSI RCATGY 2 cut(s) 18, 461
BstSCI CCNGG 3 cut(s) 186, 897, 1033
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 5 cut(s) 212, 215, 265, 751, 1050
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 3 cut(s) 377, 905, 1225
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 280, 413, 702, 1088
BtsIMutI CAGTG 3 cut(s) 192, 691, 1197
Cac8I GCNNGC 3 cut(s) 141, 671, 861
CaiI CAGNNNCTG 1 cut(s) 209
CciI TCATGA 1 cut(s) 468
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 2 cut(s) 304, 605
Csp6I GTAC 5 cut(s) 535, 979, 1111, 1188, 1202
CviAII CATG 9 cut(s) 15, 39, 395, 458, 462, 469, 800, 828, 1159
CviQI GTAC 5 cut(s) 535, 979, 1111, 1188, 1202
DdeI CTNAG 4 cut(s) 90, 576, 839, 867
DpnI GATC 3 cut(s) 350, 648, 775
DpnII GATC 3 cut(s) 348, 646, 773
EaeI YGGCCR 2 cut(s) 375, 1223
Eco130I CCWWGG 2 cut(s) 38, 640
Eco24I GRGCYC 1 cut(s) 821
Eco31I GGTCTC 1 cut(s) 914
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 304, 605
EcoO109I RGGNCCY 1 cut(s) 605
EcoRI GAATTC 1 cut(s) 916
EcoRII CCWGG 3 cut(s) 186, 897, 1033
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 2 cut(s) 38, 640
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 821
ErhI CCWWGG 2 cut(s) 38, 640
FaeI CATG 9 cut(s) 18, 42, 398, 461, 465, 472, 803, 831, 1162
FaqI GGGAC 2 cut(s) 618, 963
FatI CATG 9 cut(s) 14, 38, 394, 457, 461, 468, 799, 827, 1158
Fnu4HI GCNGC 6 cut(s) 201, 204, 279, 765, 768, 1039
FokI GGATG 4 cut(s) 287, 420, 709, 1075
FriOI GRGCYC 1 cut(s) 821
Fsp4HI GCNGC 6 cut(s) 201, 204, 279, 765, 768, 1039
FspBI CTAG 1 cut(s) 641
GluI GCNGC 6 cut(s) 201, 204, 279, 765, 768, 1039
GsaI CCCAGC 1 cut(s) 1218
GsuI CTGGAG 2 cut(s) 234, 920
HaeIII GGCC 3 cut(s) 377, 905, 1225
HapII CCGG 3 cut(s) 322, 906, 1092
Hin1II CATG 9 cut(s) 18, 42, 398, 461, 465, 472, 803, 831, 1162
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 2 cut(s) 67, 267
HinfI GANTC 4 cut(s) 20, 562, 987, 1008
HpaII CCGG 3 cut(s) 322, 906, 1092
HphI GGTGA 3 cut(s) 489, 656, 750
Hpy166II GTNNAC 2 cut(s) 304, 691
Hpy188I TCNGA 8 cut(s) 93, 229, 441, 520, 733, 870, 1150, 1172
Hpy8I GTNNAC 2 cut(s) 304, 691
HpyAV CCTTC 3 cut(s) 392, 865, 1145
HpyCH4III ACNGT 6 cut(s) 196, 223, 418, 709, 1051, 1192
HpyCH4IV ACGT 2 cut(s) 248, 977
HpyCH4V TGCA 5 cut(s) 461, 479, 827, 850, 1086
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 209, 275, 856
HpyF3I CTNAG 4 cut(s) 90, 576, 839, 867
HpySE526I ACGT 2 cut(s) 248, 977
Hsp92II CATG 9 cut(s) 18, 42, 398, 461, 465, 472, 803, 831, 1162
Kpn2I TCCGGA 1 cut(s) 1091
Kzo9I GATC 3 cut(s) 348, 646, 773
LmnI GCTCC 3 cut(s) 215, 868, 874
Lsp1109I GCAGC 5 cut(s) 212, 215, 265, 751, 1050
LweI GCATC 5 cut(s) 265, 682, 687, 1050, 1165
MaeI CTAG 1 cut(s) 641
MaeII ACGT 2 cut(s) 248, 977
MaeIII GTNAC 5 cut(s) 10, 410, 424, 703, 756
MalI GATC 3 cut(s) 350, 648, 775
MboI GATC 3 cut(s) 348, 646, 773
MboII GAAGA 4 cut(s) 121, 350, 583, 1107
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 821
MluCI AATT 3 cut(s) 379, 843, 916
MlyI GAGTC 2 cut(s) 29, 996
MmeI TCCRAC 3 cut(s) 634, 1048, 1150
Mph1103I ATGCAT 1 cut(s) 463
MroI TCCGGA 1 cut(s) 1091
MseI TTAA 4 cut(s) 33, 488, 635, 929
MslI CAYNNNNRTG 3 cut(s) 460, 684, 1163
MspA1I CMGCKG 3 cut(s) 764, 770, 1028
MspI CCGG 3 cut(s) 322, 906, 1092
MspR9I CCNGG 3 cut(s) 188, 899, 1035
MvaI CCWGG 3 cut(s) 188, 899, 1035
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 4 cut(s) 145, 209, 275, 856
NcoI CCATGG 1 cut(s) 38
NdeII GATC 3 cut(s) 348, 646, 773
NlaIII CATG 9 cut(s) 18, 42, 398, 461, 465, 472, 803, 831, 1162
NlaIV GGNNCC 4 cut(s) 78, 350, 606, 1032
NmuCI GTSAC 2 cut(s) 410, 756
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 2 cut(s) 18, 461
NspV TTCGAA 1 cut(s) 384
PagI TCATGA 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 2 cut(s) 562, 1008
Pfl23II CGTACG 1 cut(s) 978
PflMI CCANNNNNTGG 1 cut(s) 271
PfoI TCCNGGA 2 cut(s) 186, 897
PkrI GCNGC 6 cut(s) 202, 205, 280, 766, 769, 1040
PleI GAGTC 2 cut(s) 28, 995
PpsI GAGTC 2 cut(s) 28, 995
PpuMI RGGWCCY 1 cut(s) 605
Psp1406I AACGTT 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 605
Psp6I CCWGG 3 cut(s) 186, 897, 1033
PspFI CCCAGC 1 cut(s) 1214
PspGI CCWGG 3 cut(s) 186, 897, 1033
PspLI CGTACG 1 cut(s) 978
PspN4I GGNNCC 4 cut(s) 78, 350, 606, 1032
PspPI GGNCC 2 cut(s) 304, 605
PspPPI RGGWCCY 1 cut(s) 605
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 348
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 5 cut(s) 536, 980, 1112, 1189, 1203
RsaNI GTAC 5 cut(s) 535, 979, 1111, 1188, 1202
RseI CAYNNNNRTG 3 cut(s) 460, 684, 1163
SaqAI TTAA 4 cut(s) 33, 488, 635, 929
SatI GCNGC 6 cut(s) 201, 204, 279, 765, 768, 1039
Sau3AI GATC 3 cut(s) 348, 646, 773
Sau96I GGNCC 2 cut(s) 304, 605
ScaI AGTACT 1 cut(s) 1112
SchI GAGTC 2 cut(s) 29, 996
ScrFI CCNGG 3 cut(s) 188, 899, 1035
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 5 cut(s) 265, 682, 687, 1050, 1165
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 2 cut(s) 304, 605
SmiMI CAYNNNNRTG 3 cut(s) 460, 684, 1163
SmlI CTYRAG 1 cut(s) 1240
SmoI CTYRAG 1 cut(s) 1240
Sse9I AATT 3 cut(s) 379, 843, 916
SsiI CCGC 3 cut(s) 74, 768, 1028
SspMI CTAG 1 cut(s) 641
StyD4I CCNGG 3 cut(s) 186, 897, 1033
StyI CCWWGG 2 cut(s) 38, 640
TaaI ACNGT 6 cut(s) 196, 223, 418, 709, 1051, 1192
TaiI ACGT 2 cut(s) 251, 980
TaqI TCGA 6 cut(s) 153, 243, 252, 384, 967, 1232
TasI AATT 3 cut(s) 379, 843, 916
TatI WGTACW 2 cut(s) 534, 1110
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 2 cut(s) 562, 1008
Tru1I TTAA 4 cut(s) 33, 488, 635, 929
Tru9I TTAA 4 cut(s) 33, 488, 635, 929
TscAI CASTG 3 cut(s) 199, 691, 1197
TseFI GTSAC 2 cut(s) 410, 756
TseI GCWGC 5 cut(s) 200, 203, 278, 764, 1038
Tsp45I GTSAC 2 cut(s) 410, 756
TspDTI ATGAA 3 cut(s) 411, 485, 900
TspRI CASTG 3 cut(s) 199, 691, 1197
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 2 cut(s) 304, 605
XapI RAATTY 2 cut(s) 843, 916
XceI RCATGY 2 cut(s) 18, 461
XmaJI CCTAGG 1 cut(s) 640
XspI CTAG 1 cut(s) 641
ZrmI AGTACT 1 cut(s) 1112
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.