Rorug07G0203300

Aminomethyltransferase folate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005067
Physical Location & Seq
Forward (+)
17797760 .. 17800959
3200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0203300.1

Sequence Viewer

Length: 1104 bp
ATGGCTGCTGCTTCCAACATAGTCAAGTATGGGATTGTAGGGGTGGGTATGATGGGTCGAGAGCACCTCATCAATTTGTACCATCTTCGCTCTGAAGGCGTGGCCGTGGTAGCCATAGCTGACCCCCATGTTCCTTCCCAAAAACTGGCCTTGGACTTGGCTCACTCTTTCCATTGGCCTCTAAAGGTTTTTTCAGGCCATAAGGAGCTATTGGATAGTCTTCTCTGTGATGTGGTGGTTGTGTCGACTCCAAATATGACCCATCATCATATTCTAATGGATATAATTAACCACCCCAAACCCCACCATGTACTGGTGGAGAAGCCGTTGTGTACCACAGTTGCTCACTGCAAAGAGGTTATTGAAGCCGCTAGAAAGAGGCAGGATATGTTGGTGCAAGTTGGACTGGAGTATAGATACATGCCGCCTGTTGCTAAACTGATAAACATAGTGAAGGGTGGAAGTCTTGGGCAGGTCAAAATGGTGGCAATCCGAGAACACAGGTTTCCTTTCTTGGTTAAGGTCAACAATTGGAACCGGTTTAATGTTAACTCTGGGGGGACACTGGTAGAGAAGTGCTGTCACTTCTTTGATTTGATGAGGCTGTTTGCTGGAGCTAGTCCTTTTCGTGTGATGGCTTCTGGAGCCATTGATGTTAACCACAAAAATGAAATATATGATGGGAAGGTACCAGATATAATTGACAATGCATATGTTATTGTCGAATTTGATAATGGTGCTCGTGGGATGCTTGACCTTTGTATGTTTGCTGAAGGGAGTAAAAATGAGCAAGAAATATCGGTTGTTGGTGATGTCGGAAAGGGAGAGGCCTTTGTTCCTGAGAGTATTGTACGTACTGGTACTCGAGTAGGTGGGAGAGATGGTGTCAAGACTTTAAAAGCTGAGAATGATCAGATAAAATATGATGGGCTGCATCATGGATCTAGCTATTTGGAACACCTGCACTTCTTATCTGCAATTAGGGCTAAACAAGGACCTGCTGTTGATTTGCATGATGGATTAGTTTCAGTTGCCATTGGAGTTGCAGCACAACTTTCCATAGAGAAAGGCCGGTTTGTCACAATTGAAGAAGTCATGAATTAG

Protein Analysis

367

Amino Acids

40.41

Weight (kDa)

6.87

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 9 - 135 8.5e-27 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 145 - 273 1.1e-14 GFO/IDH/MocA C-terminal domain
GFO_IDH_MocA_C PF02894 151 - 361 1.8e-39 Oxidoreductase family, C-terminal alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 969
Acc36I ACCTGC 3 cut(s) 463, 969, 1006
Acc65I GGTACC 1 cut(s) 688
AccB1I GGYRCC 1 cut(s) 688
AccB7I CCANNNNNTGG 2 cut(s) 145, 313
AccI GTMKAC 1 cut(s) 245
AciI CCGC 2 cut(s) 369, 425
AclWI GGATC 1 cut(s) 949
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 2 cut(s) 114, 792
AfaI GTAC 7 cut(s) 80, 312, 334, 690, 852, 856, 862
AfiI CCNNNNNNNGG 2 cut(s) 145, 313
AgeI ACCGGT 1 cut(s) 537
AgsI TTSAA 2 cut(s) 365, 1088
AluBI AGCT 5 cut(s) 119, 208, 617, 902, 948
AluI AGCT 5 cut(s) 119, 208, 617, 902, 948
Alw21I GWGCWC 2 cut(s) 66, 742
AlwI GGATC 1 cut(s) 949
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 6 cut(s) 102, 147, 176, 196, 828, 1069
ApeKI GCWGC 4 cut(s) 5, 8, 931, 1046
ApoI RAATTY 1 cut(s) 725
AsiGI ACCGGT 1 cut(s) 537
Asp718I GGTACC 1 cut(s) 688
AspS9I GGNCC 1 cut(s) 995
AsuHPI GGTGA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 1 cut(s) 995
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BanI GGYRCC 1 cut(s) 688
BauI CACGAG 1 cut(s) 741
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 66, 742
BbvI GCAGC 2 cut(s) 918, 1058
BccI CCATC 8 cut(s) 46, 90, 270, 628, 674, 875, 920, 1010
BceAI ACGGC 2 cut(s) 89, 310
BclI TGATCA 1 cut(s) 910
BfaI CTAG 3 cut(s) 372, 618, 945
BfuAI ACCTGC 3 cut(s) 463, 969, 1006
BisI GCNGC 6 cut(s) 6, 9, 369, 425, 932, 1047
BlsI GCNGC 6 cut(s) 7, 10, 370, 426, 933, 1048
Bme18I GGWCC 1 cut(s) 995
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 1 cut(s) 995
BmiI GGNNCC 3 cut(s) 536, 646, 690
BmsI GCATC 2 cut(s) 738, 943
BpiI GAAGAC 1 cut(s) 212
BplI GAGNNNNNCTC 2 cut(s) 51, 83
BpmI CTGGAG 3 cut(s) 428, 633, 663
BsaAI YACGTR 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 105, 150
BsaWI WCCGGW 1 cut(s) 537
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 868, 898
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 313
Bse118I RCCGGY 2 cut(s) 537, 1071
Bse1I ACTGG 5 cut(s) 150, 318, 411, 570, 862
BseDI CCNNGG 2 cut(s) 105, 150
BseGI GGATG 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 145, 313
BseMII CTCAG 2 cut(s) 831, 894
BseNI ACTGG 5 cut(s) 150, 318, 411, 570, 862
BseXI GCAGC 2 cut(s) 918, 1058
BsgI GTGCAG 1 cut(s) 947
BshFI GGCC 6 cut(s) 104, 149, 178, 198, 830, 1071
BshNI GGYRCC 1 cut(s) 688
BshTI ACCGGT 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 66, 742
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 2 cut(s) 538, 1072
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 2 cut(s) 145, 313
BsmFI GGGAC 1 cut(s) 574
BsnI GGCC 6 cut(s) 104, 149, 178, 198, 830, 1071
BsoBI CYCGRG 1 cut(s) 864
Bsp1286I GDGCHC 2 cut(s) 66, 742
Bsp143I GATC 2 cut(s) 910, 941
BspACI CCGC 2 cut(s) 369, 425
BspANI GGCC 6 cut(s) 104, 149, 178, 198, 830, 1071
BspCNI CTCAG 2 cut(s) 832, 895
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 3 cut(s) 536, 646, 690
BspMI ACCTGC 3 cut(s) 463, 969, 1006
BspPI GGATC 1 cut(s) 949
BspT107I GGYRCC 1 cut(s) 688
BsrFI RCCGGY 2 cut(s) 537, 1071
BsrI ACTGG 5 cut(s) 150, 318, 411, 570, 862
BssAI RCCGGY 2 cut(s) 537, 1071
BssECI CCNNGG 2 cut(s) 105, 150
BssMI GATC 2 cut(s) 910, 941
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 741
Bst4CI ACNGT 1 cut(s) 340
BstBAI YACGTR 1 cut(s) 854
BstDEI CTNAG 2 cut(s) 840, 903
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 753
BstKTI GATC 2 cut(s) 913, 944
BstMBI GATC 2 cut(s) 910, 941
BstMWI GCNNNNNNNGC 4 cut(s) 96, 110, 644, 983
BstNSI RCATGY 1 cut(s) 424
BstSNI TACGTA 1 cut(s) 854
BstV1I GCAGC 2 cut(s) 918, 1058
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 941
BstYI RGATCY 1 cut(s) 941
BsuRI GGCC 6 cut(s) 104, 149, 178, 198, 830, 1071
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 753
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 2 cut(s) 346, 563
BveI ACCTGC 3 cut(s) 463, 969, 1006
CciI TCATGA 1 cut(s) 1095
Cfr10I RCCGGY 2 cut(s) 537, 1071
Cfr13I GGNCC 1 cut(s) 995
Csp6I GTAC 7 cut(s) 79, 311, 333, 689, 851, 855, 861
CspAI ACCGGT 1 cut(s) 537
CviAII CATG 6 cut(s) 128, 308, 421, 938, 1013, 1096
CviQI GTAC 7 cut(s) 79, 311, 333, 689, 851, 855, 861
DdeI CTNAG 2 cut(s) 840, 903
DpnI GATC 2 cut(s) 912, 943
DpnII GATC 2 cut(s) 910, 941
DraI TTTAAA 1 cut(s) 897
EaeI YGGCCR 1 cut(s) 102
Eco105I TACGTA 1 cut(s) 854
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 995
Eco57I CTGAAG 2 cut(s) 114, 792
Eco88I CYCGRG 1 cut(s) 864
EcoO109I RGGNCCY 1 cut(s) 995
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 6 cut(s) 131, 311, 424, 941, 1016, 1099
FaqI GGGAC 1 cut(s) 574
FatI CATG 6 cut(s) 127, 307, 420, 937, 1012, 1095
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 6 cut(s) 6, 9, 369, 425, 932, 1047
FokI GGATG 1 cut(s) 760
Fsp4HI GCNGC 6 cut(s) 6, 9, 369, 425, 932, 1047
FspBI CTAG 3 cut(s) 372, 618, 945
GluI GCNGC 6 cut(s) 6, 9, 369, 425, 932, 1047
GsuI CTGGAG 3 cut(s) 428, 633, 663
HaeIII GGCC 6 cut(s) 104, 149, 178, 198, 830, 1071
HapII CCGG 2 cut(s) 538, 1072
Hin1II CATG 6 cut(s) 131, 311, 424, 941, 1016, 1099
HincII GTYRAC 4 cut(s) 246, 526, 550, 658
HindII GTYRAC 4 cut(s) 246, 526, 550, 658
HinfI GANTC 1 cut(s) 247
HpaI GTTAAC 2 cut(s) 550, 658
HpaII CCGG 2 cut(s) 538, 1072
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 5 cut(s) 246, 333, 526, 550, 658
Hpy188I TCNGA 4 cut(s) 94, 494, 818, 915
Hpy188III TCNNGA 5 cut(s) 59, 642, 839, 889, 1096
Hpy8I GTNNAC 5 cut(s) 246, 333, 526, 550, 658
HpyAV CCTTC 5 cut(s) 89, 144, 448, 679, 767
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 853
HpyCH4V TGCA 8 cut(s) 351, 397, 710, 934, 964, 977, 1012, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 110, 644, 983
HpyF3I CTNAG 2 cut(s) 840, 903
HpySE526I ACGT 1 cut(s) 853
Hsp92II CATG 6 cut(s) 131, 311, 424, 941, 1016, 1099
KpnI GGTACC 1 cut(s) 692
Ksp22I TGATCA 1 cut(s) 910
KspAI GTTAAC 2 cut(s) 550, 658
Kzo9I GATC 2 cut(s) 910, 941
LmnI GCTCC 3 cut(s) 205, 614, 644
Lsp1109I GCAGC 2 cut(s) 918, 1058
LweI GCATC 2 cut(s) 738, 943
MaeI CTAG 3 cut(s) 372, 618, 945
MaeII ACGT 1 cut(s) 853
MaeIII GTNAC 2 cut(s) 581, 1078
MalI GATC 2 cut(s) 912, 943
MboI GATC 2 cut(s) 910, 941
MboII GAAGA 3 cut(s) 77, 212, 1100
MfeI CAATTG 2 cut(s) 529, 1083
MflI RGATCY 1 cut(s) 941
MhlI GDGCHC 2 cut(s) 66, 742
MluCI AATT 8 cut(s) 73, 285, 529, 699, 725, 978, 1083, 1099
MlyI GAGTC 1 cut(s) 241
MmeI TCCRAC 3 cut(s) 39, 382, 796
MnlI CCTC 6 cut(s) 77, 189, 349, 372, 594, 820
Mph1103I ATGCAT 1 cut(s) 712
MseI TTAA 6 cut(s) 288, 519, 543, 549, 657, 896
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 2 cut(s) 538, 1072
MunI CAATTG 2 cut(s) 529, 1083
MwoI GCNNNNNNNGC 4 cut(s) 96, 110, 644, 983
NdeI CATATG 1 cut(s) 712
NdeII GATC 2 cut(s) 910, 941
NlaIII CATG 6 cut(s) 131, 311, 424, 941, 1016, 1099
NlaIV GGNNCC 3 cut(s) 536, 646, 690
NmuCI GTSAC 2 cut(s) 581, 1078
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 864
PagI TCATGA 1 cut(s) 1095
PaqCI CACCTGC 1 cut(s) 969
PceI AGGCCT 1 cut(s) 830
PflMI CCANNNNNTGG 2 cut(s) 145, 313
PinAI ACCGGT 1 cut(s) 537
PkrI GCNGC 6 cut(s) 7, 10, 370, 426, 933, 1048
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 995
Psp5II RGGWCCY 1 cut(s) 995
PspN4I GGNNCC 3 cut(s) 536, 646, 690
PspPI GGNCC 1 cut(s) 995
PspPPI RGGWCCY 1 cut(s) 995
PspXI VCTCGAGB 1 cut(s) 864
PsuI RGATCY 1 cut(s) 941
RsaI GTAC 7 cut(s) 80, 312, 334, 690, 852, 856, 862
RsaNI GTAC 7 cut(s) 79, 311, 333, 689, 851, 855, 861
RseI CAYNNNNRTG 1 cut(s) 666
SalI GTCGAC 1 cut(s) 244
SaqAI TTAA 6 cut(s) 288, 519, 543, 549, 657, 896
SatI GCNGC 6 cut(s) 6, 9, 369, 425, 932, 1047
Sau3AI GATC 2 cut(s) 910, 941
Sau96I GGNCC 1 cut(s) 995
SchI GAGTC 1 cut(s) 241
SduI GDGCHC 2 cut(s) 66, 742
SfaNI GCATC 2 cut(s) 738, 943
Sfr274I CTCGAG 1 cut(s) 864
SinI GGWCC 1 cut(s) 995
SlaI CTCGAG 1 cut(s) 864
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 864
SmoI CTYRAG 1 cut(s) 864
SnaBI TACGTA 1 cut(s) 854
Sse9I AATT 8 cut(s) 73, 285, 529, 699, 725, 978, 1083, 1099
SseBI AGGCCT 1 cut(s) 830
SsiI CCGC 2 cut(s) 369, 425
SspMI CTAG 3 cut(s) 372, 618, 945
StuI AGGCCT 1 cut(s) 830
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 856
TaqI TCGA 4 cut(s) 58, 245, 723, 865
TasI AATT 8 cut(s) 73, 285, 529, 699, 725, 978, 1083, 1099
TatI WGTACW 1 cut(s) 310
TauI GCSGC 2 cut(s) 371, 427
Tru1I TTAA 6 cut(s) 288, 519, 543, 549, 657, 896
Tru9I TTAA 6 cut(s) 288, 519, 543, 549, 657, 896
TscAI CASTG 2 cut(s) 353, 570
TseFI GTSAC 2 cut(s) 581, 1078
TseI GCWGC 4 cut(s) 5, 8, 931, 1046
Tsp45I GTSAC 2 cut(s) 581, 1078
TspDTI ATGAA 1 cut(s) 684
TspRI CASTG 2 cut(s) 353, 570
Van91I CCANNNNNTGG 2 cut(s) 145, 313
VpaK11BI GGWCC 1 cut(s) 995
XapI RAATTY 1 cut(s) 725
XceI RCATGY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 864
XmiI GTMKAC 1 cut(s) 245
XspI CTAG 3 cut(s) 372, 618, 945
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.