Rroxscaffold_3G00237730

Aminomethyltransferase folate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005067
Physical Location & Seq
Forward (+)
25431228 .. 25435833
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237730.1

Sequence Viewer

Length: 1338 bp
ATGGCAAGAGTAACATGTGATTGTCTCCTTGTTAATCCATGGCGTTTATTCTCATATCATCCCCACCAAAGCTTTGCGGTTCCCTTGCTCACTCTCACCCAGACGAAGAAGCAGCAGAAGCTCACACTCTTCTCATCTTCCAAAACGAGGACCAGGGCTTTGCCATTCGACCTCTCCCCTCCTCCGATTGACCACGACCTCTTGGACACTGTGGCAGCAACTGCAGGAGCAAAAGTCTCAGATGATGGGATTATCGAAACCTTTGATAACGACGACCAAGCTTTAGATGCTGTCGATAATGGTGTTGCGGTTGCGGACCTTTCACATTTTTGCCGGATAAGAGGTTATGTGATCAAATTGGTGTCTGTTAGCGGAGAAGACCGCATCCAGTTTCTTCACAACCAAAGCACGGCCAATTTTGAATGCCTTGATGAAGGACAGGGATGTGACACCGTTTTTGTTACACCAACAGCTCGGACAATAGATATCGCACATGCATGGGTCATGAAAAATGCAGTAATGTTAATGGTTTCACCTGTTTCCAGAAGTATCTCTGAAATGCTGAAAAAATTGGCTATTGATGATGGTTTGTCTGCTTATAGTTGGAGGGACTTTCTCGAGTACATATTTTTTAATGACAAGGTAGAGATTCAAGACATCACTAAGCAAACTTGTTTCTTCGTCTTAGTGGGACCTAAAAGCAATCAACTAATGGAGGAGTTAAACCTAGGTGATCTCGTTGGACAACCACATGGCACCCATCAGCATTTCAGTGTCAATGGGATGCCTGTAACTGTGGGAGTGGGCAATGTGATATCTGAAAAAGGCTTTTCACTTTTGATGTCACCAGCTGCCGCTGGATCAGTCTGGAAAACTATTCTATCACAAGGTGCAACTCCAATGGGCTCTAATGCATGGGAAAAACTAAGAATTCTCCAAGGAAAGCCAGCTCCTCAGAAGGAGCTTACTAATGAATATAATGTCCTGGAGGCTGGTCTCTGGAATTCGATCTCCTTAAGCAAAGGGTGCTATAAGGGACAAGAGACCATATCGAGACTGATAACGTATGATGGAGTCAAGCAAAGACTTTGGGGAATCTCTCTATCAGTGCCAGCAGAACCTGGCAGCATCATTACAGTTGGTGGGAAAAAGGTTGGAAAGCTAACAAGTTGCACATCTGGAAGAAAGGAAAGTGAGTATTTTGGTTTAGGCTATATCAAGAGGCAAAATGTTTCAGAAGGAGACACGGTAATGGTTGGAGATGCTATTACTGGTACAGTGGTAGAAGTACCTTTTCTTGCTGGGCAACGGCCACCATCGAGAAGCTCAAGCTCCTGA

Protein Analysis

445

Amino Acids

48.55

Weight (kDa)

6.16

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_T PF01571 93 - 341 1.5e-19 GCVT N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 755
AciI CCGC 6 cut(s) 77, 308, 314, 372, 382, 855
AclWI GGATC 1 cut(s) 868
AcoI YGGCCR 2 cut(s) 411, 1310
AcsI RAATTY 2 cut(s) 930, 1003
AdeI CACNNNGTG 1 cut(s) 890
AfaI GTAC 3 cut(s) 623, 1276, 1290
AfiI CCNNNNNNNGG 2 cut(s) 147, 409
AflII CTTAAG 1 cut(s) 1015
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 2 cut(s) 422, 653
AjnI CCWGG 3 cut(s) 152, 984, 1120
Alw26I GTCTC 6 cut(s) 29, 241, 1001, 1037, 1048, 1236
AlwI GGATC 1 cut(s) 868
AlwNI CAGNNNCTG 2 cut(s) 221, 1121
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 2 cut(s) 411, 1310
ApeKI GCWGC 4 cut(s) 112, 215, 851, 1125
ApoI RAATTY 2 cut(s) 930, 1003
ArsI GACNNNNNNTTYG 4 cut(s) 142, 174, 440, 472
AspA2I CCTAGG 1 cut(s) 727
AspS9I GGNCC 3 cut(s) 150, 316, 692
AsuHPI GGTGA 4 cut(s) 88, 525, 743, 837
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 3 cut(s) 150, 316, 692
AvrII CCTAGG 1 cut(s) 727
BaeI ACNNNNGTAYC 2 cut(s) 1266, 1299
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 1 cut(s) 908
BbsI GAAGAC 1 cut(s) 384
BbvI GCAGC 4 cut(s) 124, 227, 838, 1137
BccI CCATC 5 cut(s) 239, 578, 768, 1064, 1324
BceAI ACGGC 2 cut(s) 426, 1325
BciT130I CCWGG 3 cut(s) 154, 986, 1122
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 6 cut(s) 29, 241, 1001, 1037, 1048, 1236
BfaI CTAG 1 cut(s) 728
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 1015
BisI GCNGC 5 cut(s) 113, 216, 852, 855, 1126
BlnI CCTAGG 1 cut(s) 727
BlsI GCNGC 5 cut(s) 114, 217, 853, 856, 1127
Bme1390I CCNGG 3 cut(s) 154, 986, 1122
Bme18I GGWCC 3 cut(s) 150, 316, 692
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 3 cut(s) 150, 316, 692
BmiI GGNNCC 3 cut(s) 81, 693, 757
BmrFI CCNGG 3 cut(s) 154, 986, 1122
BmsI GCATC 5 cut(s) 277, 393, 774, 1137, 1252
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 1007
BpuEI CTTGAG 1 cut(s) 1312
BsaI GGTCTC 2 cut(s) 1001, 1037
BsaJI CCNNGG 4 cut(s) 38, 153, 727, 937
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 1233, 1263
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 409
Bse1I ACTGG 2 cut(s) 388, 1276
Bse3DI GCAATG 1 cut(s) 814
BseBI CCWGG 3 cut(s) 154, 986, 1122
BseDI CCNNGG 4 cut(s) 38, 153, 727, 937
BseGI GGATG 4 cut(s) 58, 384, 449, 789
BseLI CCNNNNNNNGG 2 cut(s) 147, 409
BseMI GCAATG 1 cut(s) 814
BseMII CTCAG 2 cut(s) 252, 968
BseNI ACTGG 2 cut(s) 388, 1276
BseRI GAGGAG 3 cut(s) 171, 731, 942
BseXI GCAGC 4 cut(s) 124, 227, 838, 1137
BseYI CCCAGC 1 cut(s) 1301
BshFI GGCC 2 cut(s) 413, 1312
BshNI GGYRCC 1 cut(s) 755
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 3 cut(s) 623, 705, 1050
BslI CCNNNNNNNGG 2 cut(s) 147, 409
BsmAI GTCTC 6 cut(s) 29, 241, 1001, 1037, 1048, 1236
BsmFI GGGAC 3 cut(s) 623, 705, 1050
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 2 cut(s) 413, 1312
Bso31I GGTCTC 2 cut(s) 1001, 1037
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 908
Bsp143I GATC 4 cut(s) 351, 733, 860, 1008
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 6 cut(s) 77, 308, 314, 372, 382, 855
BspANI GGCC 2 cut(s) 413, 1312
BspCNI CTCAG 2 cut(s) 251, 967
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 3 cut(s) 81, 693, 757
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 1 cut(s) 868
BspT107I GGYRCC 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 1015
BspTNI GGTCTC 2 cut(s) 1001, 1037
BsrDI GCAATG 1 cut(s) 814
BsrI ACTGG 2 cut(s) 388, 1276
BssECI CCNNGG 4 cut(s) 38, 153, 727, 937
BssMI GATC 4 cut(s) 351, 733, 860, 1008
BssT1I CCWWGG 3 cut(s) 38, 727, 937
Bst2UI CCWGG 3 cut(s) 154, 986, 1122
Bst4CI ACNGT 6 cut(s) 211, 454, 796, 1138, 1249, 1279
Bst6I CTCTTC 1 cut(s) 134
BstAFI CTTAAG 1 cut(s) 1015
BstAPI GCANNNNNTGC 2 cut(s) 221, 1026
BstC8I GCNNGC 2 cut(s) 948, 1113
BstDEI CTNAG 5 cut(s) 238, 663, 685, 926, 954
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 58, 384, 449, 789
BstKTI GATC 4 cut(s) 354, 736, 863, 1011
BstMAI GTCTC 6 cut(s) 29, 241, 1001, 1037, 1048, 1236
BstMBI GATC 4 cut(s) 351, 733, 860, 1008
BstMWI GCNNNNNNNGC 4 cut(s) 118, 221, 287, 1026
BstNI CCWGG 3 cut(s) 154, 986, 1122
BstNSI RCATGY 2 cut(s) 18, 497
BstSCI CCNGG 3 cut(s) 152, 984, 1120
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 124, 227, 838, 1137
BstV2I GAAGAC 1 cut(s) 384
BsuRI GGCC 2 cut(s) 413, 1312
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 58, 384, 449, 789
BtsIMutI CAGTG 4 cut(s) 207, 778, 1113, 1284
Cac8I GCNNGC 2 cut(s) 948, 1113
CaiI CAGNNNCTG 2 cut(s) 221, 1121
CciI TCATGA 1 cut(s) 504
Cfr13I GGNCC 3 cut(s) 150, 316, 692
Csp6I GTAC 3 cut(s) 622, 1275, 1289
CviAII CATG 7 cut(s) 15, 39, 494, 498, 505, 752, 915
CviQI GTAC 3 cut(s) 622, 1275, 1289
DdeI CTNAG 5 cut(s) 238, 663, 685, 926, 954
DpnI GATC 4 cut(s) 353, 735, 862, 1010
DpnII GATC 4 cut(s) 351, 733, 860, 1008
DraIII CACNNNGTG 1 cut(s) 890
EaeI YGGCCR 2 cut(s) 411, 1310
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 3 cut(s) 38, 727, 937
Eco24I GRGCYC 1 cut(s) 908
Eco31I GGTCTC 2 cut(s) 1001, 1037
Eco32I GATATC 2 cut(s) 487, 816
Eco47I GGWCC 3 cut(s) 150, 316, 692
Eco88I CYCGRG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 692
EcoRI GAATTC 2 cut(s) 930, 1003
EcoRII CCWGG 3 cut(s) 152, 984, 1120
EcoRV GATATC 2 cut(s) 487, 816
EcoT14I CCWWGG 3 cut(s) 38, 727, 937
EcoT22I ATGCAT 2 cut(s) 499, 916
EcoT38I GRGCYC 1 cut(s) 908
ErhI CCWWGG 3 cut(s) 38, 727, 937
FaeI CATG 7 cut(s) 18, 42, 497, 501, 508, 755, 918
FaqI GGGAC 3 cut(s) 623, 705, 1050
FatI CATG 7 cut(s) 14, 38, 493, 497, 504, 751, 914
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 5 cut(s) 113, 216, 852, 855, 1126
FokI GGATG 4 cut(s) 45, 371, 456, 796
FriOI GRGCYC 1 cut(s) 908
Fsp4HI GCNGC 5 cut(s) 113, 216, 852, 855, 1126
FspBI CTAG 1 cut(s) 728
GluI GCNGC 5 cut(s) 113, 216, 852, 855, 1126
GsaI CCCAGC 1 cut(s) 1305
GsuI CTGGAG 1 cut(s) 1007
HaeIII GGCC 2 cut(s) 413, 1312
HapII CCGG 1 cut(s) 334
Hin1II CATG 7 cut(s) 18, 42, 497, 501, 508, 755, 918
HindIII AAGCTT 2 cut(s) 70, 279
HinfI GANTC 3 cut(s) 649, 1074, 1095
HpaII CCGG 1 cut(s) 334
HphI GGTGA 4 cut(s) 88, 525, 743, 837
Hpy188I TCNGA 7 cut(s) 186, 241, 477, 556, 820, 957, 1237
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 428, 952, 1232
HpyCH4III ACNGT 6 cut(s) 211, 454, 796, 1138, 1249, 1279
HpyCH4IV ACGT 1 cut(s) 1064
HpyCH4V TGCA 6 cut(s) 224, 497, 515, 893, 914, 1173
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 221, 287, 1026
HpyF3I CTNAG 5 cut(s) 238, 663, 685, 926, 954
HpySE526I ACGT 1 cut(s) 1064
Hsp92II CATG 7 cut(s) 18, 42, 497, 501, 508, 755, 918
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 4 cut(s) 351, 733, 860, 1008
LmnI GCTCC 4 cut(s) 227, 955, 961, 1337
Lsp1109I GCAGC 4 cut(s) 124, 227, 838, 1137
LweI GCATC 5 cut(s) 277, 393, 774, 1137, 1252
MaeI CTAG 1 cut(s) 728
MaeII ACGT 1 cut(s) 1064
MaeIII GTNAC 5 cut(s) 10, 446, 460, 790, 843
MalI GATC 4 cut(s) 353, 735, 862, 1010
MboI GATC 4 cut(s) 351, 733, 860, 1008
MboII GAAGA 7 cut(s) 118, 121, 129, 386, 389, 670, 1194
MhlI GDGCHC 1 cut(s) 908
MluCI AATT 5 cut(s) 356, 415, 569, 930, 1003
MlyI GAGTC 1 cut(s) 1083
MmeI TCCRAC 4 cut(s) 584, 721, 1135, 1237
Mph1103I ATGCAT 2 cut(s) 499, 916
MseI TTAA 5 cut(s) 33, 524, 633, 722, 1016
MslI CAYNNNNRTG 3 cut(s) 496, 771, 1250
MspA1I CMGCKG 2 cut(s) 851, 857
MspCI CTTAAG 1 cut(s) 1015
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 3 cut(s) 154, 986, 1122
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 3 cut(s) 154, 986, 1122
MwoI GCNNNNNNNGC 4 cut(s) 118, 221, 287, 1026
NcoI CCATGG 1 cut(s) 38
NdeII GATC 4 cut(s) 351, 733, 860, 1008
NlaIII CATG 7 cut(s) 18, 42, 497, 501, 508, 755, 918
NlaIV GGNNCC 3 cut(s) 81, 693, 757
NmuCI GTSAC 2 cut(s) 446, 843
NsiI ATGCAT 2 cut(s) 499, 916
NspI RCATGY 2 cut(s) 18, 497
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 1 cut(s) 504
PciI ACATGT 1 cut(s) 14
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 2 cut(s) 649, 1095
PfoI TCCNGGA 1 cut(s) 984
PkrI GCNGC 5 cut(s) 114, 217, 853, 856, 1127
PleI GAGTC 1 cut(s) 1082
PpsI GAGTC 1 cut(s) 1082
PpuMI RGGWCCY 1 cut(s) 692
PscI ACATGT 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 692
Psp6I CCWGG 3 cut(s) 152, 984, 1120
PspFI CCCAGC 1 cut(s) 1301
PspGI CCWGG 3 cut(s) 152, 984, 1120
PspN4I GGNNCC 3 cut(s) 81, 693, 757
PspPI GGNCC 3 cut(s) 150, 316, 692
PspPPI RGGWCCY 1 cut(s) 692
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 2 cut(s) 221, 1121
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 3 cut(s) 623, 1276, 1290
RsaNI GTAC 3 cut(s) 622, 1275, 1289
RseI CAYNNNNRTG 3 cut(s) 496, 771, 1250
SaqAI TTAA 5 cut(s) 33, 524, 633, 722, 1016
SatI GCNGC 5 cut(s) 113, 216, 852, 855, 1126
Sau3AI GATC 4 cut(s) 351, 733, 860, 1008
Sau96I GGNCC 3 cut(s) 150, 316, 692
SchI GAGTC 1 cut(s) 1083
ScrFI CCNGG 3 cut(s) 154, 986, 1122
SduI GDGCHC 1 cut(s) 908
SfaNI GCATC 5 cut(s) 277, 393, 774, 1137, 1252
SfcI CTRYAG 1 cut(s) 222
Sfr274I CTCGAG 1 cut(s) 617
SinI GGWCC 3 cut(s) 150, 316, 692
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 3 cut(s) 496, 771, 1250
SmlI CTYRAG 3 cut(s) 617, 1015, 1327
SmoI CTYRAG 3 cut(s) 617, 1015, 1327
Sse9I AATT 5 cut(s) 356, 415, 569, 930, 1003
SsiI CCGC 6 cut(s) 77, 308, 314, 372, 382, 855
SspMI CTAG 1 cut(s) 728
StyD4I CCNGG 3 cut(s) 152, 984, 1120
StyI CCWWGG 3 cut(s) 38, 727, 937
TaaI ACNGT 6 cut(s) 211, 454, 796, 1138, 1249, 1279
TaiI ACGT 1 cut(s) 1067
TaqI TCGA 7 cut(s) 168, 255, 294, 618, 1007, 1052, 1319
TasI AATT 5 cut(s) 356, 415, 569, 930, 1003
TatI WGTACW 1 cut(s) 621
TauI GCSGC 1 cut(s) 857
TfiI GAWTC 2 cut(s) 649, 1095
Tru1I TTAA 5 cut(s) 33, 524, 633, 722, 1016
Tru9I TTAA 5 cut(s) 33, 524, 633, 722, 1016
TscAI CASTG 4 cut(s) 214, 778, 1113, 1284
TseFI GTSAC 2 cut(s) 446, 843
TseI GCWGC 4 cut(s) 112, 215, 851, 1125
Tsp45I GTSAC 2 cut(s) 446, 843
TspDTI ATGAA 3 cut(s) 447, 521, 987
TspRI CASTG 4 cut(s) 214, 778, 1113, 1284
Vha464I CTTAAG 1 cut(s) 1015
VpaK11BI GGWCC 3 cut(s) 150, 316, 692
XapI RAATTY 2 cut(s) 930, 1003
XceI RCATGY 2 cut(s) 18, 497
XhoI CTCGAG 1 cut(s) 617
XmaJI CCTAGG 1 cut(s) 727
XspI CTAG 1 cut(s) 728
Zsp2I ATGCAT 2 cut(s) 499, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.