MD08G1235500.v1.1

Aminomethyltransferase folate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005067
Physical Location & Seq
Forward (+)
30302200 .. 30308598
6399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1235500.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGGCAGCACCAATAACAGCAGCAGCAGGTGATTTTCTGCTTCTCAATCCACGGCCATGGCGTTCCTTCTCCTATCTCCACCAGAACGGTGCGTTTCTCTCTACTCAGGCCAAGAACAATCAACACAACCAGAAGCAGAAGCAGAAGTTCACGCTCTCTTCCAAAACGACCAGGAGCCGCGTCGTTTCTTCCTCCTTGCCCTTCGACCTCTCCCCTCCTCCTATCGACCACGACCTCATCGACACTGTGGCAACTGCAGGAGCAAAGGTTTCTGATGATGGGATTATCGAAACTTTCGATAACGATGACCAAGCATTGGATGCGGCTGACAATGGTGTCGCGGTCGTGGACCTCTCACATTTTGGACGGATAAGAGTTAGTGGAGAGGACCGTATCCAGTTTCTTCACAACCAAACCACTGCCAATTTTGAAAGCCTTCATCAAGGACAGGGATGCGACACTGTTTTTGTTACACCAACAGCCCGGACAATAGATATTGCACATGCATGGATCATGAAAAATGCAGTAATGTTAGTTGTTTCACCTGTGACTTCGAGAAGTATCTCTGAAATGCTGCAAAAGTACATATTCTTTAATGACAAGGTAGAGATTCAAGACATCACTAAGCAAACTTGCTTCTTTGTTTTGGTAGGACCTAAAAGCAACCAACTGATGAAGGAGTTAAACCTAGGTGACCTTGTTGAACAACCATATGGCACACATCAGCATTTTAGTGTTGACGGGATGCCTATAACTGTGGGAGTGGGAAATGTCATTTCTGAAAAAGGTTTTTCATTTTTGATGTCACCAGCTACCGCTGAATCAGTATGGAAAACTATTCTGTCACAGGGTGCTATCCCGATGGGCTCTAATGCATGGGAAAAACTACGAATTTTCCAAGGAAGGCCAGCGCCTCAGAAGGAGCTTACCAATGAATATAATGTCTTGGAGGCTGGTCTCTGGAATTCAATCTCTTTAAACAAAGGGTGCTACAAAGGACAAGAGACTATAGCTAGACTCATAACATATGATGGAGTCAAGCAACGACTTTGGGGGATTTGTCTAGCAGCGCCGGCAGAGGTTGGCAGCATTCTTACAGTTGGAGGGAAAAAGGTTGGAAAGCTGACAAGTTGCACATCTGGAAAAAAGGAATCTGGATATATTGGTTTAGGCTATATCAAGAGACAATCTGTTTCGGAGGGAGACACCGTAATTGTTGGAGATGCCATTACTGGAACAGTGGTAGAAGTACCTTTTCTTGCTGGGCAACGCCCACCATCGAAAAGCTCAAGCTCTTGA

Protein Analysis

433

Amino Acids

46.94

Weight (kDa)

7.11

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_T PF01571 105 - 328 8.1e-30 GCVT N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 17
AasI GACNNNNNNGTC 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 17
AccB7I CCANNNNNTGG 1 cut(s) 316
AccII CGCG 2 cut(s) 180, 341
AciI CCGC 4 cut(s) 178, 323, 341, 816
AclWI GGATC 1 cut(s) 518
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 2 cut(s) 891, 964
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 2 cut(s) 584, 1251
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 4 cut(s) 431, 614, 704, 969
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 6 cut(s) 812, 925, 1013, 1123, 1287, 1293
AluI AGCT 6 cut(s) 812, 925, 1013, 1123, 1287, 1293
Alw26I GTCTC 4 cut(s) 962, 998, 1177, 1197
AlwI GGATC 1 cut(s) 518
AoxI GGCC 3 cut(s) 53, 108, 905
ApeKI GCWGC 6 cut(s) 5, 20, 23, 574, 1067, 1086
ApoI RAATTY 2 cut(s) 891, 964
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 1 cut(s) 435
AspA2I CCTAGG 1 cut(s) 688
AspLEI GCGC 2 cut(s) 913, 1072
AspS9I GGNCC 3 cut(s) 349, 388, 653
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 4 cut(s) 41, 534, 704, 798
AvaII GGWCC 3 cut(s) 349, 388, 653
AvrII CCTAGG 1 cut(s) 688
BanII GRGCYC 1 cut(s) 869
BbvI GCAGC 6 cut(s) 17, 32, 35, 561, 1079, 1098
BccI CCATC 4 cut(s) 272, 856, 1025, 1285
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 172
BciVI GTATCC 1 cut(s) 404
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 4 cut(s) 962, 998, 1177, 1197
BfaI CTAG 3 cut(s) 689, 1014, 1064
BfmI CTRYAG 2 cut(s) 255, 1008
BfoI RGCGCY 2 cut(s) 914, 1073
BfuAI ACCTGC 1 cut(s) 17
BfuI GTATCC 1 cut(s) 404
BisI GCNGC 8 cut(s) 6, 21, 24, 178, 324, 575, 1068, 1087
BlnI CCTAGG 1 cut(s) 688
BlsI GCNGC 8 cut(s) 7, 22, 25, 179, 325, 576, 1069, 1088
Bme1390I CCNGG 2 cut(s) 172, 484
Bme18I GGWCC 3 cut(s) 349, 388, 653
BmgT120I GGNCC 3 cut(s) 349, 388, 653
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 2 cut(s) 172, 484
BmsI GCATC 4 cut(s) 310, 443, 735, 1213
BpuEI CTTGAG 1 cut(s) 1273
BpuMI CCSGG 1 cut(s) 484
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 4 cut(s) 50, 56, 688, 898
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 1194, 1224
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse118I RCCGGY 1 cut(s) 1072
Bse1I ACTGG 2 cut(s) 397, 1237
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 50, 56, 688, 898
BseGI GGATG 3 cut(s) 325, 458, 750
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 119, 929
BseNI ACTGG 2 cut(s) 397, 1237
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 6 cut(s) 17, 32, 35, 561, 1079, 1098
BseYI CCCAGC 1 cut(s) 1262
Bsh1236I CGCG 2 cut(s) 180, 341
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 3 cut(s) 55, 110, 907
BsiEI CGRYCG 1 cut(s) 345
BsiSI CCGG 2 cut(s) 484, 1073
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 4 cut(s) 962, 998, 1177, 1197
BsmI GAATGC 1 cut(s) 1089
BsnI GGCC 3 cut(s) 55, 110, 907
Bso31I GGTCTC 1 cut(s) 962
Bsp1286I GDGCHC 1 cut(s) 869
Bsp143I GATC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 4 cut(s) 178, 323, 341, 816
BspANI GGCC 3 cut(s) 55, 110, 907
BspCNI CTCAG 2 cut(s) 118, 928
BspFNI CGCG 2 cut(s) 180, 341
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 1 cut(s) 176
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 962
BsrFI RCCGGY 1 cut(s) 1072
BsrI ACTGG 2 cut(s) 397, 1237
BssAI RCCGGY 1 cut(s) 1072
BssECI CCNNGG 4 cut(s) 50, 56, 688, 898
BssMI GATC 1 cut(s) 510
BssT1I CCWWGG 3 cut(s) 56, 688, 898
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 8 cut(s) 89, 247, 392, 463, 757, 1099, 1210, 1240
Bst6I CTCTTC 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 320
BstC8I GCNNGC 2 cut(s) 909, 1074
BstDEI CTNAG 3 cut(s) 105, 624, 915
BstDSI CCRYGG 2 cut(s) 50, 56
BstEII GGTNACC 1 cut(s) 692
BstF5I GGATG 3 cut(s) 325, 458, 750
BstFNI CGCG 2 cut(s) 180, 341
BstH2I RGCGCY 2 cut(s) 914, 1073
BstHHI GCGC 2 cut(s) 913, 1072
BstKTI GATC 1 cut(s) 513
BstMAI GTCTC 4 cut(s) 962, 998, 1177, 1197
BstMBI GATC 1 cut(s) 510
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 2 cut(s) 320, 1073
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 506
BstPI GGTNACC 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 170, 482
BstSFI CTRYAG 2 cut(s) 255, 1008
BstUI CGCG 2 cut(s) 180, 341
BstV1I GCAGC 6 cut(s) 17, 32, 35, 561, 1079, 1098
BstXI CCANNNNNNTGG 1 cut(s) 57
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 3 cut(s) 55, 110, 907
BtgI CCRYGG 2 cut(s) 50, 56
BtsCI GGATG 3 cut(s) 325, 458, 750
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 4 cut(s) 243, 417, 459, 1245
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 909, 1074
CciI TCATGA 1 cut(s) 513
CfoI GCGC 2 cut(s) 913, 1072
Cfr10I RCCGGY 1 cut(s) 1072
Cfr13I GGNCC 3 cut(s) 349, 388, 653
CseI GACGC 1 cut(s) 169
Csp6I GTAC 2 cut(s) 583, 1250
CviAII CATG 5 cut(s) 57, 503, 507, 514, 876
CviQI GTAC 2 cut(s) 583, 1250
DdeI CTNAG 3 cut(s) 105, 624, 915
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
DraI TTTAAA 1 cut(s) 978
DraIII CACNNNGTG 1 cut(s) 851
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 56, 688, 898
Eco24I GRGCYC 1 cut(s) 869
Eco31I GGTCTC 1 cut(s) 962
Eco47I GGWCC 3 cut(s) 349, 388, 653
Eco91I GGTNACC 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 653
EcoO65I GGTNACC 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 3 cut(s) 56, 688, 898
EcoT22I ATGCAT 2 cut(s) 508, 877
EcoT38I GRGCYC 1 cut(s) 869
ErhI CCWWGG 3 cut(s) 56, 688, 898
FaeI CATG 5 cut(s) 60, 506, 510, 517, 879
FatI CATG 5 cut(s) 56, 502, 506, 513, 875
FauNDI CATATG 2 cut(s) 712, 1027
Fnu4HI GCNGC 8 cut(s) 6, 21, 24, 178, 324, 575, 1068, 1087
FokI GGATG 3 cut(s) 332, 465, 757
FriOI GRGCYC 1 cut(s) 869
Fsp4HI GCNGC 8 cut(s) 6, 21, 24, 178, 324, 575, 1068, 1087
FspBI CTAG 3 cut(s) 689, 1014, 1064
GlaI GCGC 2 cut(s) 912, 1071
GluI GCNGC 8 cut(s) 6, 21, 24, 178, 324, 575, 1068, 1087
GsaI CCCAGC 1 cut(s) 1266
HaeII RGCGCY 2 cut(s) 914, 1073
HaeIII GGCC 3 cut(s) 55, 110, 907
HapII CCGG 2 cut(s) 484, 1073
HgaI GACGC 1 cut(s) 169
HhaI GCGC 2 cut(s) 913, 1072
Hin1II CATG 5 cut(s) 60, 506, 510, 517, 879
Hin6I GCGC 2 cut(s) 911, 1070
HinP1I GCGC 2 cut(s) 911, 1070
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 5 cut(s) 610, 821, 1017, 1035, 1151
HpaII CCGG 2 cut(s) 484, 1073
HphI GGTGA 4 cut(s) 41, 534, 704, 798
Hpy166II GTNNAC 3 cut(s) 150, 349, 739
Hpy188I TCNGA 5 cut(s) 274, 568, 781, 918, 1198
Hpy188III TCNNGA 9 cut(s) 514, 555, 614, 859, 961, 1140, 1155, 1180, 1296
Hpy8I GTNNAC 3 cut(s) 150, 349, 739
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 6 cut(s) 76, 211, 446, 670, 897, 913
HpyCH4III ACNGT 8 cut(s) 89, 247, 392, 463, 757, 1099, 1210, 1240
HpyCH4V TGCA 7 cut(s) 257, 500, 506, 524, 577, 875, 1134
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 1073
HpyF3I CTNAG 3 cut(s) 105, 624, 915
Hsp92II CATG 5 cut(s) 60, 506, 510, 517, 879
HspAI GCGC 2 cut(s) 911, 1070
KroI GCCGGC 1 cut(s) 1072
KroNI GCCGGC 1 cut(s) 1074
Kzo9I GATC 1 cut(s) 510
LmnI GCTCC 3 cut(s) 174, 260, 922
Lsp1109I GCAGC 6 cut(s) 17, 32, 35, 561, 1079, 1098
LweI GCATC 4 cut(s) 310, 443, 735, 1213
MaeI CTAG 3 cut(s) 689, 1014, 1064
MaeIII GTNAC 5 cut(s) 469, 547, 692, 804, 843
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 3 cut(s) 150, 180, 395
MhlI GDGCHC 1 cut(s) 869
MluCI AATT 4 cut(s) 424, 891, 964, 1212
MlyI GAGTC 2 cut(s) 1011, 1044
MmeI TCCRAC 3 cut(s) 1081, 1096, 1198
Mph1103I ATGCAT 2 cut(s) 508, 877
MroNI GCCGGC 1 cut(s) 1072
MroXI GAANNNNTTC 1 cut(s) 435
MseI TTAA 3 cut(s) 594, 683, 977
MslI CAYNNNNRTG 3 cut(s) 55, 505, 732
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 2 cut(s) 484, 1073
MspR9I CCNGG 2 cut(s) 172, 484
Mva1269I GAATGC 1 cut(s) 1089
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 2 cut(s) 180, 341
MwoI GCNNNNNNNGC 2 cut(s) 320, 1073
NaeI GCCGGC 1 cut(s) 1074
NciI CCSGG 1 cut(s) 484
NcoI CCATGG 1 cut(s) 56
NdeI CATATG 2 cut(s) 712, 1027
NdeII GATC 1 cut(s) 510
NgoMIV GCCGGC 1 cut(s) 1072
NlaIII CATG 5 cut(s) 60, 506, 510, 517, 879
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 4 cut(s) 547, 692, 804, 843
NsiI ATGCAT 2 cut(s) 508, 877
NspI RCATGY 1 cut(s) 506
PagI TCATGA 1 cut(s) 513
PaqCI CACCTGC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 3 cut(s) 58, 237, 294
PctI GAATGC 1 cut(s) 1089
PdiI GCCGGC 1 cut(s) 1074
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 3 cut(s) 610, 821, 1151
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 8 cut(s) 7, 22, 25, 179, 325, 576, 1069, 1088
PleI GAGTC 2 cut(s) 1011, 1043
PpsI GAGTC 2 cut(s) 1011, 1043
PpuMI RGGWCCY 1 cut(s) 653
Psp5II RGGWCCY 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 170
PspEI GGTNACC 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 1262
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 3 cut(s) 349, 388, 653
PspPPI RGGWCCY 1 cut(s) 653
PstI CTGCAG 1 cut(s) 259
RsaI GTAC 2 cut(s) 584, 1251
RsaNI GTAC 2 cut(s) 583, 1250
RseI CAYNNNNRTG 3 cut(s) 55, 505, 732
SaqAI TTAA 3 cut(s) 594, 683, 977
SatI GCNGC 8 cut(s) 6, 21, 24, 178, 324, 575, 1068, 1087
Sau3AI GATC 1 cut(s) 510
Sau96I GGNCC 3 cut(s) 349, 388, 653
SchI GAGTC 2 cut(s) 1011, 1044
ScrFI CCNGG 2 cut(s) 172, 484
SduI GDGCHC 1 cut(s) 869
SfaNI GCATC 4 cut(s) 310, 443, 735, 1213
SfcI CTRYAG 2 cut(s) 255, 1008
SinI GGWCC 3 cut(s) 349, 388, 653
SmiMI CAYNNNNRTG 3 cut(s) 55, 505, 732
SmlI CTYRAG 1 cut(s) 1288
SmoI CTYRAG 1 cut(s) 1288
Sse9I AATT 4 cut(s) 424, 891, 964, 1212
SsiI CCGC 4 cut(s) 178, 323, 341, 816
SspMI CTAG 3 cut(s) 689, 1014, 1064
StyD4I CCNGG 2 cut(s) 170, 482
StyI CCWWGG 3 cut(s) 56, 688, 898
TaaI ACNGT 8 cut(s) 89, 247, 392, 463, 757, 1099, 1210, 1240
TaqI TCGA 7 cut(s) 204, 225, 240, 288, 297, 554, 1280
TasI AATT 4 cut(s) 424, 891, 964, 1212
TatI WGTACW 1 cut(s) 582
TauI GCSGC 2 cut(s) 180, 326
TfiI GAWTC 3 cut(s) 610, 821, 1151
Tru1I TTAA 3 cut(s) 594, 683, 977
Tru9I TTAA 3 cut(s) 594, 683, 977
TscAI CASTG 4 cut(s) 250, 424, 466, 1245
TseFI GTSAC 4 cut(s) 547, 692, 804, 843
TseI GCWGC 6 cut(s) 5, 20, 23, 574, 1067, 1086
Tsp45I GTSAC 4 cut(s) 547, 692, 804, 843
TspDTI ATGAA 5 cut(s) 428, 530, 689, 783, 948
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 4 cut(s) 250, 424, 466, 1245
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 3 cut(s) 349, 388, 653
XapI RAATTY 2 cut(s) 891, 964
XceI RCATGY 1 cut(s) 506
XmaJI CCTAGG 1 cut(s) 688
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 3 cut(s) 689, 1014, 1064
Zsp2I ATGCAT 2 cut(s) 508, 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.