FvH4_5g36220

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015640
Physical Location & Seq
Reverse (-)
26548459 .. 26549523
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36220.t1

Sequence Viewer

Length: 1065 bp
ATGGACCGGAAGATCAACTTCATCGACGTTCTCAAAGACAAGGCCTCCATCCTCCGCGCCTCCTTCTCCATCAACCGCCATGTGTCATCCATCCACGTATCCATCCTCCGCGCCACTTCCCACGACCCGTCGTCGCCGCCGTCGGAGGATAGAATCAACGACATCCTCGAGCTAGGGAAGTCGTCGCGTCTCGCTGCGTGCACCTGCATTGACATCCTTATGGACCGCCTCCATGCCACCCAGAACGCTTTCGTCGCGCTGAAATGTCTGTTCACCGTACATAACATAGTGTCGAGAGGTTCGTTGGTTTTCAGGGACCAGATTTCTTATCATCCCAGTTTCGGAGGGCGTAACTTCTTGAACATGGCCATGTTCAGCGACAAATCGGATATGGACACGTGGGAGTTTTCGTTATGGGTGCGGTGGTACGCCGCCGTGGTGGAACAGAATATGACGGCGGCGAGGGTGATCGGTTATTACCTCAACCCGCCACACAAGTTTGCGGAATACCAAACAAAAACGCTGCCGGTGTTGAATAATGATCTGGTGAATGAGATTGAAGCGCTTATAGAATTTGTTGTGAGAATCTCCGACCCGCCGGAATCGTTGAACCTGCAGCGGAACAATTTGGTTTACGAGATCGTGAAGGCGGCGAGCGAGGATTACAGGGCGGTTCAGTATGAAATTTCGGTACGGGTTAAAGAAACGGAGGAAAGGGTGGAGAGACTGTGCCTGTCCGAGTTGACTCGGGTGGTGGACGCGCTGAGGAGGCTGGAGGATTGCAAGGAGAGGCTGGTGTTGCTGTTCGTGAACCGGAAGAGGAACGAGGGGTTGTGGGAGTCGGTTCGGAAAACGAGTTCGGAGCTGGCGGAGACGAAAGCGCAGAGAGAGGAGAAGAGGCTGGTAGTGAGGACCATGAGTAGGAACAGAGGGCGAGACGATATGAGCGAGTCGACTCAGTGCTGGAACCCGTTTCTTGAACCAGGGCAGTTGCTTCTGTTACCTTCGGGCCGCGGCGGAGGAGGAGGGTGGCTGGGGATGGGCCCCAGCCCAATAGCCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

355

Amino Acids

40.39

Weight (kDa)

7.64

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 31 - 160 2.7e-12 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36220
malus_domestica MD08G1190800.v1.1 MD15G1378100.v1.1
prunus_persica Prupe.1G524700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238041
rosa_laevigata RLG00000000945
rosa_multiflora Rmu_co8251785.1_g000001 Rmu_sc0002586.1_g000013 Rmu_sc0021174.1_g000001
rosa_roxburghii Rroxscaffold_3G00223430
rosa_rugosa Rorug07G0310200
rosa_samantha Rh7AG465400 Rh7BG436200 Rh7CG483600 Rh7DG452100
rosa_wichuraiana Rw7G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 212
Acc36I ACCTGC 2 cut(s) 212, 621
AccI GTMKAC 1 cut(s) 953
AccII CGCG 6 cut(s) 57, 111, 187, 257, 761, 1014
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 2 cut(s) 572, 684
AcvI CACGTG 1 cut(s) 399
AfaI GTAC 3 cut(s) 279, 428, 693
AfeI AGCGCT 1 cut(s) 564
AfiI CCNNNNNNNGG 2 cut(s) 341, 921
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 5 cut(s) 361, 535, 560, 610, 980
AhdI GACNNNNNGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 982
AloI GAACNNNNNNTCC 2 cut(s) 841, 873
AluBI AGCT 2 cut(s) 172, 865
AluI AGCT 2 cut(s) 172, 865
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 4 cut(s) 194, 718, 866, 930
Alw44I GTGCAC 1 cut(s) 199
Ama87I CYCGRG 2 cut(s) 167, 747
Aor51HI AGCGCT 1 cut(s) 564
AoxI GGCC 4 cut(s) 42, 366, 1009, 1042
ApaI GGGCCC 1 cut(s) 1046
ApaLI GTGCAC 1 cut(s) 199
ApeKI GCWGC 3 cut(s) 194, 523, 616
ApoI RAATTY 2 cut(s) 572, 684
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 6 cut(s) 59, 113, 259, 565, 763, 883
AspS9I GGNCC 7 cut(s) 4, 223, 316, 912, 1009, 1042, 1043
AsuHPI GGTGA 3 cut(s) 265, 478, 559
AvaI CYCGRG 2 cut(s) 167, 747
AvaII GGWCC 4 cut(s) 4, 223, 316, 912
BaeGI GKGCMC 2 cut(s) 203, 1046
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 1 cut(s) 1046
BbrPI CACGTG 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 764
BbvI GCAGC 3 cut(s) 181, 510, 628
BccI CCATC 5 cut(s) 56, 77, 98, 110, 1033
BceAI ACGGC 4 cut(s) 124, 419, 471, 1043
BciT130I CCWGG 1 cut(s) 984
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 4 cut(s) 194, 718, 866, 930
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 614
BfoI RGCGCY 1 cut(s) 566
BfuAI ACCTGC 2 cut(s) 212, 621
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 9 cut(s) 137, 195, 432, 459, 524, 617, 651, 1012, 1015
BlsI GCNGC 9 cut(s) 138, 196, 433, 460, 525, 618, 652, 1013, 1016
Bme1390I CCNGG 1 cut(s) 984
Bme18I GGWCC 4 cut(s) 4, 223, 316, 912
BmeRI GACNNNNNGTC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 167, 747
BmgT120I GGNCC 7 cut(s) 4, 223, 316, 912, 1009, 1042, 1043
BmiI GGNNCC 4 cut(s) 317, 968, 1044, 1045
BmrFI CCNGG 1 cut(s) 984
BmrI ACTGGG 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 794
Bpu10I CCTNAGC 1 cut(s) 764
BsaAI YACGTR 2 cut(s) 97, 399
BsaJI CCNNGG 3 cut(s) 435, 983, 1012
BsaWI WCCGGW 2 cut(s) 6, 813
BsaXI ACNNNNNCTCC 6 cut(s) 29, 59, 395, 425, 713, 743
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 921
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 984
BseDI CCNNGG 3 cut(s) 435, 983, 1012
BseGI GGATG 8 cut(s) 48, 86, 90, 102, 162, 213, 331, 1044
BseLI CCNNNNNNNGG 2 cut(s) 341, 921
BseMII CTCAG 2 cut(s) 755, 971
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 4 cut(s) 781, 905, 1035, 1038
BseSI GKGCMC 2 cut(s) 203, 1046
BseXI GCAGC 3 cut(s) 181, 510, 628
BseYI CCCAGC 2 cut(s) 1033, 1046
Bsh1236I CGCG 6 cut(s) 57, 111, 187, 257, 761, 1014
BshFI GGCC 4 cut(s) 44, 368, 1011, 1044
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 2 cut(s) 167, 747
BsiSI CCGG 4 cut(s) 7, 527, 599, 814
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 341, 921
BsmAI GTCTC 4 cut(s) 194, 718, 866, 930
BsmBI CGTCTC 3 cut(s) 194, 866, 930
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 4 cut(s) 44, 368, 1011, 1044
BsoBI CYCGRG 2 cut(s) 167, 747
Bsp120I GGGCCC 1 cut(s) 1042
Bsp1286I GDGCHC 2 cut(s) 203, 1046
Bsp143I GATC 4 cut(s) 12, 468, 541, 639
BspANI GGCC 4 cut(s) 44, 368, 1011, 1044
BspCNI CTCAG 2 cut(s) 756, 970
BspFNI CGCG 6 cut(s) 57, 111, 187, 257, 761, 1014
BspLI GGNNCC 4 cut(s) 317, 968, 1044, 1045
BspMAI CTGCAG 1 cut(s) 618
BspMI ACCTGC 2 cut(s) 212, 621
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 336
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 3 cut(s) 435, 983, 1012
BssMI GATC 4 cut(s) 12, 468, 541, 639
Bst2UI CCWGG 1 cut(s) 984
Bst4CI ACNGT 2 cut(s) 277, 729
Bst6I CTCTTC 2 cut(s) 812, 890
BstBAI YACGTR 2 cut(s) 97, 399
BstC8I GCNNGC 3 cut(s) 199, 655, 867
BstDEI CTNAG 2 cut(s) 764, 957
BstDSI CCRYGG 2 cut(s) 435, 1012
BstF5I GGATG 8 cut(s) 48, 86, 90, 102, 162, 213, 331, 1044
BstFNI CGCG 6 cut(s) 57, 111, 187, 257, 761, 1014
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 6 cut(s) 59, 113, 259, 565, 763, 883
BstKTI GATC 4 cut(s) 15, 471, 544, 642
BstMAI GTCTC 4 cut(s) 194, 718, 866, 930
BstMBI GATC 4 cut(s) 12, 468, 541, 639
BstMWI GCNNNNNNNGC 3 cut(s) 254, 769, 799
BstNI CCWGG 1 cut(s) 984
BstSCI CCNGG 1 cut(s) 982
BstSFI CTRYAG 1 cut(s) 614
BstSLI GKGCMC 2 cut(s) 203, 1046
BstUI CGCG 6 cut(s) 57, 111, 187, 257, 761, 1014
BstV1I GCAGC 3 cut(s) 181, 510, 628
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 4 cut(s) 44, 368, 1011, 1044
BtgI CCRYGG 2 cut(s) 435, 1012
BtsCI GGATG 8 cut(s) 48, 86, 90, 102, 162, 213, 331, 1044
BtsIMutI CAGTG 1 cut(s) 965
BveI ACCTGC 2 cut(s) 212, 621
Cac8I GCNNGC 3 cut(s) 199, 655, 867
CfoI GCGC 6 cut(s) 59, 113, 259, 565, 763, 883
Cfr10I RCCGGY 1 cut(s) 526
Cfr13I GGNCC 7 cut(s) 4, 223, 316, 912, 1009, 1042, 1043
Cfr42I CCGCGG 1 cut(s) 1015
CseI GACGC 2 cut(s) 176, 767
Csp6I GTAC 3 cut(s) 278, 427, 692
CviAII CATG 5 cut(s) 80, 233, 364, 370, 916
CviQI GTAC 3 cut(s) 278, 427, 692
DdeI CTNAG 2 cut(s) 764, 957
DpnI GATC 4 cut(s) 14, 470, 543, 641
DpnII GATC 4 cut(s) 12, 468, 541, 639
DriI GACNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 812, 890
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 2 cut(s) 812, 890
EciI GGCGGA 2 cut(s) 884, 1032
Eco147I AGGCCT 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 1046
Eco47I GGWCC 4 cut(s) 4, 223, 316, 912
Eco47III AGCGCT 1 cut(s) 564
Eco72I CACGTG 1 cut(s) 399
Eco88I CYCGRG 2 cut(s) 167, 747
EcoO109I RGGNCCY 1 cut(s) 1043
EcoRII CCWGG 1 cut(s) 982
EcoT38I GRGCYC 1 cut(s) 1046
Esp3I CGTCTC 3 cut(s) 194, 866, 930
FaeI CATG 5 cut(s) 83, 236, 367, 373, 919
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 329
FatI CATG 5 cut(s) 79, 232, 363, 369, 915
FauI CCCGC 2 cut(s) 495, 603
FblI GTMKAC 1 cut(s) 953
Fnu4HI GCNGC 9 cut(s) 137, 195, 432, 459, 524, 617, 651, 1012, 1015
FokI GGATG 8 cut(s) 35, 73, 77, 89, 149, 200, 318, 1051
FriOI GRGCYC 1 cut(s) 1046
Fsp4HI GCNGC 9 cut(s) 137, 195, 432, 459, 524, 617, 651, 1012, 1015
FspBI CTAG 1 cut(s) 173
GlaI GCGC 6 cut(s) 58, 112, 258, 564, 762, 882
GluI GCNGC 9 cut(s) 137, 195, 432, 459, 524, 617, 651, 1012, 1015
GsaI CCCAGC 2 cut(s) 1037, 1050
GsuI CTGGAG 1 cut(s) 794
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 4 cut(s) 44, 368, 1011, 1044
HapII CCGG 4 cut(s) 7, 527, 599, 814
HgaI GACGC 2 cut(s) 176, 767
HhaI GCGC 6 cut(s) 59, 113, 259, 565, 763, 883
Hin1II CATG 5 cut(s) 83, 236, 367, 373, 919
Hin6I GCGC 6 cut(s) 57, 111, 257, 563, 761, 881
HinP1I GCGC 6 cut(s) 57, 111, 257, 563, 761, 881
HincII GTYRAC 2 cut(s) 744, 954
HindII GTYRAC 2 cut(s) 744, 954
HinfI GANTC 7 cut(s) 153, 585, 602, 745, 839, 950, 955
HpaII CCGG 4 cut(s) 7, 527, 599, 814
HphI GGTGA 3 cut(s) 265, 478, 559
Hpy166II GTNNAC 7 cut(s) 201, 273, 634, 744, 757, 811, 954
Hpy188I TCNGA 7 cut(s) 145, 344, 388, 592, 739, 849, 862
Hpy188III TCNNGA 5 cut(s) 294, 358, 643, 808, 977
Hpy8I GTNNAC 7 cut(s) 201, 273, 634, 744, 757, 811, 954
Hpy99I CGWCG 6 cut(s) 29, 133, 136, 145, 187, 257
HpyAV CCTTC 3 cut(s) 73, 640, 1014
HpyCH4III ACNGT 2 cut(s) 277, 729
HpyCH4IV ACGT 3 cut(s) 27, 96, 398
HpyCH4V TGCA 4 cut(s) 201, 207, 616, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 769, 799
HpyF3I CTNAG 2 cut(s) 764, 957
HpySE526I ACGT 3 cut(s) 27, 96, 398
Hsp92II CATG 5 cut(s) 83, 236, 367, 373, 919
HspAI GCGC 6 cut(s) 57, 111, 257, 563, 761, 881
KspI CCGCGG 1 cut(s) 1015
Kzo9I GATC 4 cut(s) 12, 468, 541, 639
LmnI GCTCC 1 cut(s) 862
Lsp1109I GCAGC 3 cut(s) 181, 510, 628
MaeI CTAG 1 cut(s) 173
MaeII ACGT 3 cut(s) 27, 96, 398
MaeIII GTNAC 2 cut(s) 350, 999
MalI GATC 4 cut(s) 14, 470, 543, 641
MboI GATC 4 cut(s) 12, 468, 541, 639
MboII GAAGA 3 cut(s) 22, 829, 907
MhlI GDGCHC 2 cut(s) 203, 1046
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 3 cut(s) 572, 625, 684
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 4 cut(s) 739, 848, 949, 959
MmeI TCCRAC 2 cut(s) 123, 615
Mox20I TGGCCA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 248
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 699
MslI CAYNNNNRTG 2 cut(s) 218, 368
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 619, 1014
MspI CCGG 4 cut(s) 7, 527, 599, 814
MspR9I CCNGG 1 cut(s) 984
MvaI CCWGG 1 cut(s) 984
MvnI CGCG 6 cut(s) 57, 111, 187, 257, 761, 1014
MwoI GCNNNNNNNGC 3 cut(s) 254, 769, 799
NdeII GATC 4 cut(s) 12, 468, 541, 639
NlaIII CATG 5 cut(s) 83, 236, 367, 373, 919
NlaIV GGNNCC 4 cut(s) 317, 968, 1044, 1045
PaeR7I CTCGAG 1 cut(s) 167
PaqCI CACCTGC 1 cut(s) 212
PceI AGGCCT 1 cut(s) 44
PcsI WCGNNNNNNNCGW 4 cut(s) 137, 165, 299, 945
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 153, 585, 602
PkrI GCNGC 9 cut(s) 138, 196, 433, 460, 525, 618, 652, 1013, 1016
PleI GAGTC 4 cut(s) 739, 847, 949, 958
PmaCI CACGTG 1 cut(s) 399
PmlI CACGTG 1 cut(s) 399
PpsI GAGTC 4 cut(s) 739, 847, 949, 958
Ppu21I YACGTR 2 cut(s) 97, 399
Psp6I CCWGG 1 cut(s) 982
PspCI CACGTG 1 cut(s) 399
PspFI CCCAGC 2 cut(s) 1033, 1046
PspGI CCWGG 1 cut(s) 982
PspN4I GGNNCC 4 cut(s) 317, 968, 1044, 1045
PspOMI GGGCCC 1 cut(s) 1042
PspPI GGNCC 7 cut(s) 4, 223, 316, 912, 1009, 1042, 1043
PspXI VCTCGAGB 1 cut(s) 167
PstI CTGCAG 1 cut(s) 618
RsaI GTAC 3 cut(s) 279, 428, 693
RsaNI GTAC 3 cut(s) 278, 427, 692
RseI CAYNNNNRTG 2 cut(s) 218, 368
SacII CCGCGG 1 cut(s) 1015
SalI GTCGAC 1 cut(s) 952
SaqAI TTAA 1 cut(s) 699
SatI GCNGC 9 cut(s) 137, 195, 432, 459, 524, 617, 651, 1012, 1015
Sau3AI GATC 4 cut(s) 12, 468, 541, 639
Sau96I GGNCC 7 cut(s) 4, 223, 316, 912, 1009, 1042, 1043
SchI GAGTC 4 cut(s) 739, 848, 949, 959
ScrFI CCNGG 1 cut(s) 984
SduI GDGCHC 2 cut(s) 203, 1046
SfcI CTRYAG 1 cut(s) 614
Sfr274I CTCGAG 1 cut(s) 167
Sfr303I CCGCGG 1 cut(s) 1015
SgrBI CCGCGG 1 cut(s) 1015
SinI GGWCC 4 cut(s) 4, 223, 316, 912
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 218, 368
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 3 cut(s) 572, 625, 684
SseBI AGGCCT 1 cut(s) 44
SspMI CTAG 1 cut(s) 173
StuI AGGCCT 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 982
TaaI ACNGT 2 cut(s) 277, 729
TaiI ACGT 3 cut(s) 30, 99, 401
TaqI TCGA 4 cut(s) 24, 168, 293, 953
TasI AATT 3 cut(s) 572, 625, 684
TauI GCSGC 6 cut(s) 139, 434, 461, 653, 1014, 1017
TfiI GAWTC 3 cut(s) 153, 585, 602
Tru1I TTAA 1 cut(s) 699
Tru9I TTAA 1 cut(s) 699
TscAI CASTG 1 cut(s) 965
TseI GCWGC 3 cut(s) 194, 523, 616
TspDTI ATGAA 2 cut(s) 10, 696
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 1 cut(s) 965
VneI GTGCAC 1 cut(s) 199
VpaK11BI GGWCC 4 cut(s) 4, 223, 316, 912
XapI RAATTY 2 cut(s) 572, 684
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 953
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.