MD15G1378100.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015640
Physical Location & Seq
Forward (+)
46351734 .. 46352823
1090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1378100.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCTCGATTCAAATTCATCGGCATTCTGAAAGACAGAGCCTCAATCCTCAAAGCGACTCTGACCATCAACCGTCGAGTCTCCTCCGTACACCTCGCTGTCCTCCGTGCCACCACACACGACCCGTCAAGGCCGCCATCGGAGACACGAATCGCCTCCGTGCTCGCCCTAGGGCTTTCCTCCCGCCTCACTGCATGCGCATGCATTGATGCTCTCATGGACCGGCTCCACGTCACTCGGAGCGCCTTTGTTGCCCTAAAATGTCTCCTCACGATTCAAAACATTATATCAAAAGGGTCTTTTATTCTCAAAGACCAGCTTGCGTACTACCCTTGTTTTGGGGGATACAATTTCTTGAACCTCTCCATGTTTTGTGACAATTCCGATTTTTGTATGTTGGAATATTCGTCTTGGGTCAGATGGTACGCCGGTGTGGTCGAGCAGAATCTGATGGTGTCTAGAGCAATTGGATATTATCTCAGTTCGTCGAAAAACGAAGGTAACAAAAGAGACAAGGAAGAGAAGGCACTTGCTCTTTTGGACTCAGATTTGGCAATGGAGATTGAAGTGCTTGTGGAATTTGTGGTGCGAATTTGTGACGCCCCTGATTCATTAGAACTTCAGAAGAACAATTTGGTGTACGAAGTGGTGAGAGCCACGGGCGAAGATTACAGGTCGGTTCAGCATGAAATTGTTGTCCGAGTCAAGGAAGTTGGAGACAGAATCGACTCAGTCGAGGGTTTGCGTTCGGCTGAGTTGACTCGGTTAATTGACTCGTTTGAGAGGCTGGAGGGTTGCAAGGGGAAGTTAATGCTGTTGTTTGTGAACAGGAAGAGGAATGATGGGTTATGGGATTTGGTGAAAGAGACCAAGGCTAGGCTTGTGGAGATGAAGGAGCAGCGAGAGGAGAGGCTGGTGGTTTTTCCAGGGACGAACGAGTTGGCCGAGTCGACTCAATGTTGGAACCCATTTCTTGAACCAGGACAGTTGTTGCTGTTACCATCTGGTGGTGGGTGGTTGGGCTTCGGACCGACACCAATAGCCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

350

Amino Acids

39.28

Weight (kDa)

6.91

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 32 - 166 6.5e-09 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36220
malus_domestica MD08G1190800.v1.1 MD15G1378100.v1.1
prunus_persica Prupe.1G524700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238041
rosa_laevigata RLG00000000945
rosa_multiflora Rmu_co8251785.1_g000001 Rmu_sc0002586.1_g000013 Rmu_sc0021174.1_g000001
rosa_roxburghii Rroxscaffold_3G00223430
rosa_rugosa Rorug07G0310200
rosa_samantha Rh7AG465400 Rh7BG436200 Rh7CG483600 Rh7DG452100
rosa_wichuraiana Rw7G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 199
AccB7I CCANNNNNTGG 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 950
AciI CCGC 2 cut(s) 134, 184
AcoI YGGCCR 1 cut(s) 942
AcsI RAATTY 3 cut(s) 14, 578, 591
AcuI CTGAAG 1 cut(s) 605
AcyI GRCGYC 1 cut(s) 600
AfaI GTAC 4 cut(s) 90, 326, 425, 641
AfiI CCNNNNNNNGG 4 cut(s) 338, 706, 876, 1007
AgsI TTSAA 5 cut(s) 13, 278, 358, 566, 977
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 925, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 1 cut(s) 319
AluI AGCT 1 cut(s) 319
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 6 cut(s) 85, 137, 269, 504, 711, 860
AlwNI CAGNNNCTG 1 cut(s) 448
AoxI GGCC 2 cut(s) 131, 942
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 3 cut(s) 14, 578, 591
AspA2I CCTAGG 1 cut(s) 169
AspLEI GCGC 2 cut(s) 200, 245
AspS9I GGNCC 2 cut(s) 220, 1028
AsuHPI GGTGA 2 cut(s) 661, 871
AvaII GGWCC 2 cut(s) 220, 1028
AvrII CCTAGG 1 cut(s) 169
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 910
BccI CCATC 6 cut(s) 74, 145, 414, 445, 836, 1009
BceAI ACGGC 1 cut(s) 1028
BciT130I CCWGG 2 cut(s) 927, 981
BciVI GTATCC 1 cut(s) 338
BcoDI GTCTC 6 cut(s) 85, 137, 269, 504, 711, 860
BfaI CTAG 3 cut(s) 170, 459, 876
BfoI RGCGCY 1 cut(s) 246
BfuI GTATCC 1 cut(s) 338
BisI GCNGC 2 cut(s) 134, 899
BlnI CCTAGG 1 cut(s) 169
BlsI GCNGC 2 cut(s) 135, 900
Bme1390I CCNGG 2 cut(s) 927, 981
Bme18I GGWCC 2 cut(s) 220, 1028
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 2 cut(s) 220, 1028
BmiI GGNNCC 2 cut(s) 227, 965
BmrFI CCNGG 2 cut(s) 927, 981
BmsI GCATC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 600
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 4 cut(s) 169, 657, 870, 926
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 706, 876, 1007
Bse118I RCCGGY 2 cut(s) 222, 428
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 2 cut(s) 927, 981
BseDI CCNNGG 4 cut(s) 169, 657, 870, 926
BseLI CCNNNNNNNGG 4 cut(s) 338, 706, 876, 1007
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 4 cut(s) 493, 558, 744, 744
BseRI GAGGAG 3 cut(s) 73, 257, 920
BseXI GCAGC 1 cut(s) 910
BshFI GGCC 2 cut(s) 133, 944
BsiHKAI GWGCWC 1 cut(s) 165
BsiSI CCGG 2 cut(s) 223, 429
BslFI GGGAC 1 cut(s) 943
BslI CCNNNNNNNGG 4 cut(s) 338, 706, 876, 1007
BsmAI GTCTC 6 cut(s) 85, 137, 269, 504, 711, 860
BsmFI GGGAC 1 cut(s) 943
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 2 cut(s) 133, 944
Bso31I GGTCTC 1 cut(s) 860
Bsp1286I GDGCHC 1 cut(s) 165
BspACI CCGC 2 cut(s) 134, 184
BspANI GGCC 2 cut(s) 133, 944
BspCNI CTCAG 4 cut(s) 492, 557, 743, 745
BspLI GGNNCC 2 cut(s) 227, 965
BspTNI GGTCTC 1 cut(s) 860
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 2 cut(s) 222, 428
BssAI RCCGGY 2 cut(s) 222, 428
BssECI CCNNGG 4 cut(s) 169, 657, 870, 926
BssNI GRCGYC 1 cut(s) 600
BssT1I CCWWGG 2 cut(s) 169, 870
Bst2UI CCWGG 2 cut(s) 927, 981
Bst4CI ACNGT 2 cut(s) 74, 987
Bst6I CTCTTC 2 cut(s) 513, 827
BstACI GRCGYC 1 cut(s) 600
BstC8I GCNNGC 4 cut(s) 165, 196, 202, 321
BstDEI CTNAG 4 cut(s) 479, 544, 730, 753
BstDSI CCRYGG 1 cut(s) 657
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 2 cut(s) 200, 245
BstMAI GTCTC 6 cut(s) 85, 137, 269, 504, 711, 860
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 2 cut(s) 927, 981
BstNSI RCATGY 2 cut(s) 198, 204
BstSCI CCNGG 2 cut(s) 925, 979
BstV1I GCAGC 1 cut(s) 910
BsuI GTATCC 1 cut(s) 338
BsuRI GGCC 2 cut(s) 133, 944
BtgI CCRYGG 1 cut(s) 657
BtrI CACGTC 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 4 cut(s) 165, 196, 202, 321
CaiI CAGNNNCTG 1 cut(s) 448
CfoI GCGC 2 cut(s) 200, 245
Cfr10I RCCGGY 2 cut(s) 222, 428
Cfr13I GGNCC 2 cut(s) 220, 1028
CpoI CGGWCCG 1 cut(s) 1028
CseI GACGC 1 cut(s) 608
Csp6I GTAC 4 cut(s) 89, 325, 424, 640
CspI CGGWCCG 1 cut(s) 1028
CviAII CATG 5 cut(s) 195, 201, 217, 367, 686
CviQI GTAC 4 cut(s) 89, 325, 424, 640
DdeI CTNAG 4 cut(s) 479, 544, 730, 753
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 2 cut(s) 513, 827
EarI CTCTTC 2 cut(s) 513, 827
Eco130I CCWWGG 2 cut(s) 169, 870
Eco31I GGTCTC 1 cut(s) 860
Eco47I GGWCC 2 cut(s) 220, 1028
Eco57I CTGAAG 1 cut(s) 605
EcoRII CCWGG 2 cut(s) 925, 979
EcoT14I CCWWGG 2 cut(s) 169, 870
EcoT22I ATGCAT 1 cut(s) 206
ErhI CCWWGG 2 cut(s) 169, 870
FaeI CATG 5 cut(s) 198, 204, 220, 370, 689
FaiI YATR 8 cut(s) 196, 202, 218, 287, 368, 395, 687, 850
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FaqI GGGAC 1 cut(s) 943
FatI CATG 5 cut(s) 194, 200, 216, 366, 685
FauI CCCGC 1 cut(s) 191
FblI GTMKAC 1 cut(s) 950
Fnu4HI GCNGC 2 cut(s) 134, 899
Fsp4HI GCNGC 2 cut(s) 134, 899
FspAI RTGCGCAY 1 cut(s) 199
FspBI CTAG 3 cut(s) 170, 459, 876
FspI TGCGCA 1 cut(s) 199
GlaI GCGC 2 cut(s) 199, 244
GluI GCNGC 2 cut(s) 134, 899
GsuI CTGGAG 1 cut(s) 809
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 2 cut(s) 133, 944
HapII CCGG 2 cut(s) 223, 429
HgaI GACGC 1 cut(s) 608
HhaI GCGC 2 cut(s) 200, 245
Hin1I GRCGYC 1 cut(s) 600
Hin1II CATG 5 cut(s) 198, 204, 220, 370, 689
Hin6I GCGC 2 cut(s) 198, 243
HinP1I GCGC 2 cut(s) 198, 243
HincII GTYRAC 2 cut(s) 759, 951
HindII GTYRAC 2 cut(s) 759, 951
HpaII CCGG 2 cut(s) 223, 429
HphI GGTGA 2 cut(s) 661, 871
Hpy166II GTNNAC 5 cut(s) 91, 640, 759, 826, 951
Hpy188III TCNNGA 4 cut(s) 271, 355, 459, 974
Hpy8I GTNNAC 5 cut(s) 91, 640, 759, 826, 951
Hpy99I CGWCG 2 cut(s) 78, 490
HpyAV CCTTC 3 cut(s) 491, 517, 886
HpyCH4III ACNGT 2 cut(s) 74, 987
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 194, 204, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 4 cut(s) 479, 544, 730, 753
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 600
Hsp92II CATG 5 cut(s) 198, 204, 220, 370, 689
HspAI GCGC 2 cut(s) 198, 243
LmnI GCTCC 3 cut(s) 231, 240, 895
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 1 cut(s) 199
MaeI CTAG 3 cut(s) 170, 459, 876
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 5 cut(s) 232, 374, 500, 596, 996
MboII GAAGA 4 cut(s) 530, 637, 677, 844
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 9 cut(s) 14, 349, 379, 465, 578, 591, 631, 690, 768
MlyI GAGTC 9 cut(s) 52, 87, 536, 711, 722, 754, 767, 946, 956
MmeI TCCRAC 3 cut(s) 378, 694, 941
Mph1103I ATGCAT 1 cut(s) 206
MseI TTAA 2 cut(s) 767, 809
MslI CAYNNNNRTG 1 cut(s) 199
MspI CCGG 2 cut(s) 223, 429
MspR9I CCNGG 2 cut(s) 927, 981
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 2 cut(s) 927, 981
MwoI GCNNNNNNNGC 1 cut(s) 251
NlaIII CATG 5 cut(s) 198, 204, 220, 370, 689
NlaIV GGNNCC 2 cut(s) 227, 965
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 3 cut(s) 232, 374, 596
NsbI TGCGCA 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 206
NspI RCATGY 2 cut(s) 198, 204
PaeI GCATGC 2 cut(s) 198, 204
PcsI WCGNNNNNNNCGW 2 cut(s) 732, 942
PctI GAATGC 1 cut(s) 24
PfeI GAWTC 6 cut(s) 9, 150, 274, 445, 608, 723
PflFI GACNNNGTC 1 cut(s) 731
PflMI CCANNNNNTGG 1 cut(s) 1007
PkrI GCNGC 2 cut(s) 135, 900
PleI GAGTC 9 cut(s) 52, 86, 536, 710, 722, 754, 767, 946, 955
PpsI GAGTC 9 cut(s) 52, 86, 536, 710, 722, 754, 767, 946, 955
Psp6I CCWGG 2 cut(s) 925, 979
PspGI CCWGG 2 cut(s) 925, 979
PspN4I GGNNCC 2 cut(s) 227, 965
PspPI GGNCC 2 cut(s) 220, 1028
PstNI CAGNNNCTG 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 731
RsaI GTAC 4 cut(s) 90, 326, 425, 641
RsaNI GTAC 4 cut(s) 89, 325, 424, 640
RseI CAYNNNNRTG 1 cut(s) 199
Rsr2I CGGWCCG 1 cut(s) 1028
RsrII CGGWCCG 1 cut(s) 1028
SalI GTCGAC 1 cut(s) 949
SaqAI TTAA 2 cut(s) 767, 809
SatI GCNGC 2 cut(s) 134, 899
Sau96I GGNCC 2 cut(s) 220, 1028
SchI GAGTC 9 cut(s) 52, 87, 536, 711, 722, 754, 767, 946, 956
ScrFI CCNGG 2 cut(s) 927, 981
SduI GDGCHC 1 cut(s) 165
SetI ASST 6 cut(s) 96, 234, 321, 363, 502, 677
SfaNI GCATC 1 cut(s) 199
SgrAI CRCCGGYG 1 cut(s) 428
SinI GGWCC 2 cut(s) 220, 1028
SmiMI CAYNNNNRTG 1 cut(s) 199
SphI GCATGC 2 cut(s) 198, 204
Sse9I AATT 9 cut(s) 14, 349, 379, 465, 578, 591, 631, 690, 768
SsiI CCGC 2 cut(s) 134, 184
SspI AATATT 1 cut(s) 404
SspMI CTAG 3 cut(s) 170, 459, 876
StyD4I CCNGG 2 cut(s) 925, 979
StyI CCWWGG 2 cut(s) 169, 870
TaaI ACNGT 2 cut(s) 74, 987
TaiI ACGT 1 cut(s) 234
TaqI TCGA 7 cut(s) 7, 76, 438, 488, 726, 735, 950
TaqII GACCGA 1 cut(s) 1045
TasI AATT 9 cut(s) 14, 349, 379, 465, 578, 591, 631, 690, 768
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 6 cut(s) 9, 150, 274, 445, 608, 723
Tru1I TTAA 2 cut(s) 767, 809
Tru9I TTAA 2 cut(s) 767, 809
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 3 cut(s) 232, 374, 596
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 3 cut(s) 232, 374, 596
TspDTI ATGAA 4 cut(s) 7, 600, 702, 905
TspGWI ACGGA 3 cut(s) 76, 95, 148
TspRI CASTG 1 cut(s) 196
Tth111I GACNNNGTC 1 cut(s) 731
Van91I CCANNNNNTGG 1 cut(s) 1007
VpaK11BI GGWCC 2 cut(s) 220, 1028
XapI RAATTY 3 cut(s) 14, 578, 591
XbaI TCTAGA 1 cut(s) 458
XceI RCATGY 2 cut(s) 198, 204
XmaJI CCTAGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 950
XspI CTAG 3 cut(s) 170, 459, 876
Zsp2I ATGCAT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.