MD08G1190800.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015640
Physical Location & Seq
Forward (+)
24173736 .. 24174785
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1190800.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCCCGAATCAAATTCATCGGCATCCTGAAAGACAAAGCCTCAATCCTCAAAGCGACTCTATGCATCAACGGCCGAGTCTCCGTACACCTCGCCGTCCTCCGAGCCACCACCCATGACCCGTCAAGGCCGCCTTCGGAGACACGAATCGCCTCCGTGCTCGCCCTAGGGCTTTCCTCCCGCCTCACTGCATGCGCATGCATCGAGGCCCTCATGGACCGACTCCACGTCACTCGGAGCGCCTTTGTTGCCCTAAAATGCCTCCTCACAATTCATAACATTATATCAAAAGGGTCTTTTATTCTCAAAGACCAGCTTGCTTATTACCCTTGTTTTGGGGGATACAATTTTTTGAATCTCTCCATGTTTTGTGACAATTCCGATTTGTGCATGCTGGAATATTCATCTTGGGTCAGATGGTACGCCGGTGTGATCGAGCAGAATCTCATGGTGTCTAGAGCAATTGGGTATTATCTCAATTCGACAAAAACAGATGGTAATAAAAGAGACAAGGAAGAGAGGGCACTTGCTCTTTTGGACTCGGATTTGGCAATGGAGATTGAAGTGCTTGTGGAATTTGTGGTACGAATTTGCGACGCGCCCGATTCATTAGAACTTCAGAAGAACAATTTAGTATACGAAGTGGTGAGAGCCGCGGGCGAAGATTACAGGTCGATTCAGCGTGAAATTGTTGTCCGAGTCAAGGAAGTTGGAGATAGAATCGACTCAGTCGAGGGTTTGAGTTCGGATGAGTTGACTCGGTTGATTGACGCGTTGGAGAGGCTGGAGAGTTGCAAGGGGAAGTTGATGCTATTGTTTGTGAACAGGAAGAGGAATGATGGGTTGTGGGAGTTGGTGAAGGAGACGAAGGCTAGGCTTGTGGAGATGAAGGAGAAGCGAGAGGAGAAGAGGGTGGTGGTTTTTATGGGTAGGAACGAGTCAGCCGAGTCGACTCAATGTTGGAACCCATTTCTTGAACCAGGACAGTTGTTGCTGTTACCATCTGGTGGTGCGTGGGTGGGTTTGGGACCGACACCAATAGCCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

350

Amino Acids

39.19

Weight (kDa)

7.49

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 31 - 199 3.8e-11 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36220
malus_domestica MD08G1190800.v1.1 MD15G1378100.v1.1
prunus_persica Prupe.1G524700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238041
rosa_laevigata RLG00000000945
rosa_multiflora Rmu_co8251785.1_g000001 Rmu_sc0002586.1_g000013 Rmu_sc0021174.1_g000001
rosa_roxburghii Rroxscaffold_3G00223430
rosa_rugosa Rorug07G0310200
rosa_samantha Rh7AG465400 Rh7BG436200 Rh7CG483600 Rh7DG452100
rosa_wichuraiana Rw7G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 196
AccB7I CCANNNNNTGG 1 cut(s) 1007
AccI GTMKAC 2 cut(s) 636, 950
AccII CGCG 3 cut(s) 599, 656, 773
AciI CCGC 4 cut(s) 131, 181, 654, 656
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 3 cut(s) 14, 575, 588
AcuI CTGAAG 1 cut(s) 602
AfaI GTAC 3 cut(s) 87, 422, 585
AfiI CCNNNNNNNGG 3 cut(s) 335, 703, 1007
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 3 cut(s) 355, 563, 977
AhdI GACNNNNNGTC 1 cut(s) 227
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 979
AluBI AGCT 1 cut(s) 316
AluI AGCT 1 cut(s) 316
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 4 cut(s) 85, 134, 501, 857
AoxI GGCC 4 cut(s) 3, 73, 128, 207
ApoI RAATTY 3 cut(s) 14, 575, 588
AspA2I CCTAGG 1 cut(s) 166
AspLEI GCGC 3 cut(s) 197, 242, 601
AspS9I GGNCC 4 cut(s) 4, 208, 217, 1028
AsuHPI GGTGA 2 cut(s) 658, 868
AvaII GGWCC 2 cut(s) 217, 1028
AvrII CCTAGG 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 526
BaeI ACNNNNGTAYC 2 cut(s) 412, 445
Bbv12I GWGCWC 1 cut(s) 162
BccI CCATC 4 cut(s) 411, 488, 833, 1009
BceAI ACGGC 3 cut(s) 80, 88, 1028
BciT130I CCWGG 1 cut(s) 981
BciVI GTATCC 1 cut(s) 335
BcoDI GTCTC 4 cut(s) 85, 134, 501, 857
BfaI CTAG 3 cut(s) 167, 456, 873
BfoI RGCGCY 1 cut(s) 243
BfuI GTATCC 1 cut(s) 335
BisI GCNGC 2 cut(s) 131, 654
BlnI CCTAGG 1 cut(s) 166
BlsI GCNGC 2 cut(s) 132, 655
Bme1390I CCNGG 1 cut(s) 981
Bme18I GGWCC 2 cut(s) 217, 1028
BmeRI GACNNNNNGTC 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 4 cut(s) 4, 208, 217, 1028
BmiI GGNNCC 2 cut(s) 965, 1029
BmrFI CCNGG 1 cut(s) 981
BmsI GCATC 4 cut(s) 33, 75, 210, 798
BpmI CTGGAG 1 cut(s) 806
BsaJI CCNNGG 2 cut(s) 166, 654
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 703, 1007
Bse118I RCCGGY 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 981
BseDI CCNNGG 2 cut(s) 166, 654
BseGI GGATG 2 cut(s) 24, 754
BseLI CCNNNNNNNGG 3 cut(s) 335, 703, 1007
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 1 cut(s) 741
BseRI GAGGAG 2 cut(s) 254, 917
BseSI GKGCMC 1 cut(s) 526
BseX3I CGGCCG 1 cut(s) 73
Bsh1236I CGCG 3 cut(s) 599, 656, 773
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 4 cut(s) 5, 75, 130, 209
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 1041
BslI CCNNNNNNNGG 3 cut(s) 335, 703, 1007
BsmAI GTCTC 4 cut(s) 85, 134, 501, 857
BsmBI CGTCTC 1 cut(s) 857
BsmFI GGGAC 1 cut(s) 1041
BsnI GGCC 4 cut(s) 5, 75, 130, 209
Bsp1286I GDGCHC 2 cut(s) 162, 526
Bsp143I GATC 1 cut(s) 432
BspACI CCGC 4 cut(s) 131, 181, 654, 656
BspANI GGCC 4 cut(s) 5, 75, 130, 209
BspCNI CTCAG 1 cut(s) 740
BspFNI CGCG 3 cut(s) 599, 656, 773
BspLI GGNNCC 2 cut(s) 965, 1029
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 2 cut(s) 166, 654
BssMI GATC 1 cut(s) 432
BssNAI GTATAC 1 cut(s) 637
BssT1I CCWWGG 1 cut(s) 166
Bst1107I GTATAC 1 cut(s) 637
Bst2UI CCWGG 1 cut(s) 981
Bst4CI ACNGT 1 cut(s) 987
Bst6I CTCTTC 3 cut(s) 510, 824, 902
BstC8I GCNNGC 6 cut(s) 162, 193, 199, 318, 392, 658
BstDEI CTNAG 1 cut(s) 727
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 24, 754
BstFNI CGCG 3 cut(s) 599, 656, 773
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 3 cut(s) 197, 242, 601
BstKTI GATC 1 cut(s) 435
BstMAI GTCTC 4 cut(s) 85, 134, 501, 857
BstMBI GATC 1 cut(s) 432
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 2 cut(s) 72, 248
BstNI CCWGG 1 cut(s) 981
BstNSI RCATGY 3 cut(s) 195, 201, 394
BstSCI CCNGG 1 cut(s) 979
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 3 cut(s) 599, 656, 773
BstZ17I GTATAC 1 cut(s) 637
BstZI CGGCCG 1 cut(s) 73
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 4 cut(s) 5, 75, 130, 209
BtgI CCRYGG 1 cut(s) 654
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 2 cut(s) 24, 754
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 6 cut(s) 162, 193, 199, 318, 392, 658
CfoI GCGC 3 cut(s) 197, 242, 601
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 4 cut(s) 4, 208, 217, 1028
Cfr42I CCGCGG 1 cut(s) 657
CseI GACGC 2 cut(s) 605, 779
Csp6I GTAC 3 cut(s) 86, 421, 584
CviAII CATG 7 cut(s) 116, 192, 198, 214, 364, 391, 448
CviQI GTAC 3 cut(s) 86, 421, 584
DdeI CTNAG 1 cut(s) 727
DpnI GATC 1 cut(s) 434
DpnII GATC 1 cut(s) 432
DriI GACNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 73
EagI CGGCCG 1 cut(s) 73
Eam1104I CTCTTC 3 cut(s) 510, 824, 902
Eam1105I GACNNNNNGTC 1 cut(s) 227
EarI CTCTTC 3 cut(s) 510, 824, 902
EclXI CGGCCG 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 166
Eco47I GGWCC 2 cut(s) 217, 1028
Eco52I CGGCCG 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 979
EcoT14I CCWWGG 1 cut(s) 166
EcoT22I ATGCAT 2 cut(s) 68, 203
ErhI CCWWGG 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 857
FaeI CATG 7 cut(s) 119, 195, 201, 217, 367, 394, 451
FalI AAGNNNNNCTT 4 cut(s) 118, 150, 300, 332
FaqI GGGAC 1 cut(s) 1041
FatI CATG 7 cut(s) 115, 191, 197, 213, 363, 390, 447
FauI CCCGC 2 cut(s) 188, 649
FblI GTMKAC 2 cut(s) 636, 950
Fnu4HI GCNGC 2 cut(s) 131, 654
FokI GGATG 2 cut(s) 11, 761
Fsp4HI GCNGC 2 cut(s) 131, 654
FspAI RTGCGCAY 1 cut(s) 196
FspBI CTAG 3 cut(s) 167, 456, 873
FspI TGCGCA 1 cut(s) 196
GlaI GCGC 3 cut(s) 196, 241, 600
GluI GCNGC 2 cut(s) 131, 654
GsuI CTGGAG 1 cut(s) 806
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 4 cut(s) 5, 75, 130, 209
HapII CCGG 1 cut(s) 426
HgaI GACGC 2 cut(s) 605, 779
HhaI GCGC 3 cut(s) 197, 242, 601
Hin1II CATG 7 cut(s) 119, 195, 201, 217, 367, 394, 451
Hin6I GCGC 3 cut(s) 195, 240, 599
HinP1I GCGC 3 cut(s) 195, 240, 599
HincII GTYRAC 2 cut(s) 756, 951
HindII GTYRAC 2 cut(s) 756, 951
HpaII CCGG 1 cut(s) 426
HphI GGTGA 2 cut(s) 658, 868
Hpy166II GTNNAC 5 cut(s) 88, 637, 756, 823, 951
Hpy188I TCNGA 9 cut(s) 104, 139, 237, 382, 416, 544, 621, 698, 748
Hpy188III TCNNGA 3 cut(s) 28, 456, 974
Hpy8I GTNNAC 5 cut(s) 88, 637, 756, 823, 951
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 4 cut(s) 144, 853, 862, 883
HpyCH4III ACNGT 1 cut(s) 987
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 5 cut(s) 66, 191, 201, 390, 795
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 248
HpyF3I CTNAG 1 cut(s) 727
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 7 cut(s) 119, 195, 201, 217, 367, 394, 451
HspAI GCGC 3 cut(s) 195, 240, 599
KspI CCGCGG 1 cut(s) 657
Kzo9I GATC 1 cut(s) 432
LmnI GCTCC 1 cut(s) 237
LweI GCATC 4 cut(s) 33, 75, 210, 798
MaeI CTAG 3 cut(s) 167, 456, 873
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 3 cut(s) 229, 371, 996
MalI GATC 1 cut(s) 434
MboI GATC 1 cut(s) 432
MboII GAAGA 5 cut(s) 527, 634, 674, 841, 919
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 2 cut(s) 162, 526
MluI ACGCGT 1 cut(s) 771
MmeI TCCRAC 3 cut(s) 691, 756, 941
Mph1103I ATGCAT 2 cut(s) 68, 203
MslI CAYNNNNRTG 1 cut(s) 196
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 981
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 981
MvnI CGCG 3 cut(s) 599, 656, 773
MwoI GCNNNNNNNGC 2 cut(s) 72, 248
NdeII GATC 1 cut(s) 432
NlaIII CATG 7 cut(s) 119, 195, 201, 217, 367, 394, 451
NlaIV GGNNCC 2 cut(s) 965, 1029
NmeAIII GCCGAG 2 cut(s) 101, 970
NmuCI GTSAC 2 cut(s) 229, 371
NsbI TGCGCA 1 cut(s) 196
NsiI ATGCAT 2 cut(s) 68, 203
NspI RCATGY 3 cut(s) 195, 201, 394
PaeI GCATGC 3 cut(s) 195, 201, 394
PcsI WCGNNNNNNNCGW 2 cut(s) 729, 942
PfeI GAWTC 7 cut(s) 9, 147, 355, 442, 605, 676, 720
PflFI GACNNNGTC 1 cut(s) 728
PflMI CCANNNNNTGG 1 cut(s) 1007
PkrI GCNGC 2 cut(s) 132, 655
Psp6I CCWGG 1 cut(s) 979
PspGI CCWGG 1 cut(s) 979
PspN4I GGNNCC 2 cut(s) 965, 1029
PspPI GGNCC 4 cut(s) 4, 208, 217, 1028
PsyI GACNNNGTC 1 cut(s) 728
RsaI GTAC 3 cut(s) 87, 422, 585
RsaNI GTAC 3 cut(s) 86, 421, 584
RseI CAYNNNNRTG 1 cut(s) 196
SacII CCGCGG 1 cut(s) 657
SalI GTCGAC 1 cut(s) 949
SatI GCNGC 2 cut(s) 131, 654
Sau3AI GATC 1 cut(s) 432
Sau96I GGNCC 4 cut(s) 4, 208, 217, 1028
ScrFI CCNGG 1 cut(s) 981
SduI GDGCHC 2 cut(s) 162, 526
SetI ASST 4 cut(s) 93, 231, 318, 674
SfaNI GCATC 4 cut(s) 33, 75, 210, 798
Sfr303I CCGCGG 1 cut(s) 657
SgrAI CRCCGGYG 1 cut(s) 425
SgrBI CCGCGG 1 cut(s) 657
SinI GGWCC 2 cut(s) 217, 1028
SmiMI CAYNNNNRTG 1 cut(s) 196
SphI GCATGC 3 cut(s) 195, 201, 394
SsiI CCGC 4 cut(s) 131, 181, 654, 656
SspI AATATT 1 cut(s) 401
SspMI CTAG 3 cut(s) 167, 456, 873
StyD4I CCNGG 1 cut(s) 979
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 1 cut(s) 987
TaiI ACGT 1 cut(s) 231
TaqI TCGA 7 cut(s) 204, 435, 482, 674, 723, 732, 950
TaqII GACCGA 2 cut(s) 234, 1045
TauI GCSGC 2 cut(s) 133, 656
TfiI GAWTC 7 cut(s) 9, 147, 355, 442, 605, 676, 720
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 229, 371
Tsp45I GTSAC 2 cut(s) 229, 371
TspDTI ATGAA 5 cut(s) 7, 263, 393, 597, 902
TspGWI ACGGA 2 cut(s) 73, 145
TspRI CASTG 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 728
Van91I CCANNNNNTGG 1 cut(s) 1007
VpaK11BI GGWCC 2 cut(s) 217, 1028
XapI RAATTY 3 cut(s) 14, 575, 588
XbaI TCTAGA 1 cut(s) 455
XceI RCATGY 3 cut(s) 195, 201, 394
XmaJI CCTAGG 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 636, 950
XspI CTAG 3 cut(s) 167, 456, 873
Zsp2I ATGCAT 2 cut(s) 68, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.