FvH4_5g36720

PCO_ADO

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018048
Physical Location & Seq
Forward (+)
26927919 .. 26929369
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36720.t1

Sequence Viewer

Length: 735 bp
ATGGAGTCGGGTACAATGGAGAAAACCAAAATACAAATGTTATACGAAGCCTGCAGGGTTATATTTTCTCAGAAAGAGCTTCCAACTTTCAAAGAAATTCTCTGGCTCAGAAATCTCATTGGCACATTTGCAGCAATAGATTTTGGAATCGATGAACATGGCTCGTGTGGATCTCCATCTTCTAGCCCTAACAGTGATAAGGGATTGATTTGTGGGCAAGGCATCTCCCAAATCACTTACATTCATGTTTATGAATGCGAACAATTTTCGATCGGAGTGTTTTGTTTTCCGGCAGGAGGCAGGCTTCCCCTTCATGATCACCCTGAAATGACTGTGTTTAGTAAACTCCTCTACGGTTCTTGTTATGTTAAAGCTTACGATTGGGTGAAGGTCGAAAGTTCTCCTGGATTCCCTACATTTGGATTAGGAGGCAAGGTTTTGGAAGGCATTATGAGTGCTCCATGTGAAACGTCTGTTCTATTTCCAAGAAGTGGTGGAAATATTCACTCTTTTACTGCGTTAACTCCTTGTGCTATCTTGGATGTATCGACTCCACCATACTCTGAAGAGCTTGGAAGGCCTTCTACTTACTTCATGGAATTTCCTATTCCTTCCTTACCAGGCTATATGATCCTCGAGGAACAAGAACTACCGGAAGACCTAGTAGTTGAAGGAGCACCATATCTGGGTCCTCATATTGATGTTGATAATGATGACGACAACGACCGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

245

Amino Acids

26.87

Weight (kDa)

4.54

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 72 - 233 4.9e-53 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018048)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36720
malus_domestica MD15G1373300.v1.1
prunus_persica Prupe.1G521700_v2.0.a1
pyrus_communis pycom15g33420
rosa_chinensis RchiOBHm_Chr7g0242701
rosa_laevigata RLG00000000886
rosa_roxburghii Rroxscaffold_3G00222570
rosa_rugosa Rorug07G0316400
rosa_samantha Rh7AG471500 Rh7DG457000
rosa_wichuraiana Rw7G039220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 635
AccB7I CCANNNNNTGG 1 cut(s) 491
AclWI GGATC 2 cut(s) 178, 625
AcsI RAATTY 2 cut(s) 96, 599
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
AgsI TTSAA 2 cut(s) 91, 671
AjnI CCWGG 2 cut(s) 403, 619
AluBI AGCT 3 cut(s) 79, 374, 571
AluI AGCT 3 cut(s) 79, 374, 571
Alw21I GWGCWC 2 cut(s) 460, 679
AlwI GGATC 2 cut(s) 178, 625
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 1 cut(s) 578
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 2 cut(s) 96, 599
Asp700I GAANNNNTTC 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 689
AsuHPI GGTGA 2 cut(s) 311, 397
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 1 cut(s) 689
BarI GAAGNNNNNNTAC 2 cut(s) 568, 600
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 2 cut(s) 460, 679
BbvI GCAGC 1 cut(s) 143
BccI CCATC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 405, 621
BclI TGATCA 1 cut(s) 316
BfaI CTAG 2 cut(s) 183, 662
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 2 cut(s) 405, 621
Bme18I GGWCC 1 cut(s) 689
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 689
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 2 cut(s) 405, 621
BmsI GCATC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 663
Bsa29I ATCGAT 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
Bse8I GATNNNNATC 1 cut(s) 175
BseBI CCWGG 2 cut(s) 405, 621
BseCI ATCGAT 1 cut(s) 150
BseGI GGATG 1 cut(s) 547
BseJI GATNNNNATC 1 cut(s) 175
BseLI CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
BseMII CTCAG 2 cut(s) 83, 121
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 143
Bsh1285I CGRYCG 2 cut(s) 273, 727
BshFI GGCC 1 cut(s) 580
BshVI ATCGAT 1 cut(s) 150
BsiEI CGRYCG 2 cut(s) 273, 727
BsiHKAI GWGCWC 2 cut(s) 460, 679
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 2 cut(s) 290, 653
BslI CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 580
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 460, 679
Bsp143I GATC 4 cut(s) 170, 270, 316, 630
BspANI GGCC 1 cut(s) 580
BspCNI CTCAG 2 cut(s) 82, 120
BspDI ATCGAT 1 cut(s) 150
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 690
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 178, 625
BspQI GCTCTTC 1 cut(s) 561
BssMI GATC 4 cut(s) 170, 270, 316, 630
BssSI CACGAG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 405, 621
Bst4CI ACNGT 4 cut(s) 194, 334, 356, 728
Bst6I CTCTTC 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 52, 302
BstDEI CTNAG 2 cut(s) 69, 107
BstF5I GGATG 1 cut(s) 547
BstKTI GATC 4 cut(s) 173, 273, 319, 633
BstMBI GATC 4 cut(s) 170, 270, 316, 630
BstMCI CGRYCG 2 cut(s) 273, 727
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 2 cut(s) 405, 621
BstSCI CCNGG 2 cut(s) 403, 619
BstSFI CTRYAG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 143
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 150
BsuRI GGCC 1 cut(s) 580
BsuTUI ATCGAT 1 cut(s) 150
BtsCI GGATG 1 cut(s) 547
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 52, 302
CciI TCATGA 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 689
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 1 cut(s) 12
CviAII CATG 5 cut(s) 158, 245, 314, 462, 595
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 2 cut(s) 69, 107
DpnI GATC 4 cut(s) 172, 272, 318, 632
DpnII GATC 4 cut(s) 170, 270, 316, 630
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco147I AGGCCT 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 689
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 635
EcoO109I RGGNCCY 1 cut(s) 689
EcoRII CCWGG 2 cut(s) 403, 619
FaeI CATG 5 cut(s) 161, 248, 317, 465, 598
FatI CATG 5 cut(s) 157, 244, 313, 461, 594
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 554
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 2 cut(s) 183, 662
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 1 cut(s) 580
HapII CCGG 2 cut(s) 290, 653
Hin1II CATG 5 cut(s) 161, 248, 317, 465, 598
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 4 cut(s) 5, 147, 408, 550
HpaI GTTAAC 1 cut(s) 522
HpaII CCGG 2 cut(s) 290, 653
HphI GGTGA 2 cut(s) 311, 397
Hpy166II GTNNAC 2 cut(s) 344, 522
Hpy188I TCNGA 4 cut(s) 72, 110, 275, 565
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 2 cut(s) 344, 522
HpyAV CCTTC 7 cut(s) 320, 382, 437, 570, 591, 621, 665
HpyCH4III ACNGT 4 cut(s) 194, 334, 356, 728
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 2 cut(s) 54, 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 2 cut(s) 69, 107
HpySE526I ACGT 1 cut(s) 470
Hsp92II CATG 5 cut(s) 161, 248, 317, 465, 598
Ksp22I TGATCA 1 cut(s) 316
KspAI GTTAAC 1 cut(s) 522
Kzo9I GATC 4 cut(s) 170, 270, 316, 630
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 463, 674
Lsp1109I GCAGC 1 cut(s) 143
LweI GCATC 1 cut(s) 231
MaeI CTAG 2 cut(s) 183, 662
MaeII ACGT 1 cut(s) 470
MalI GATC 4 cut(s) 172, 272, 318, 632
MboI GATC 4 cut(s) 170, 270, 316, 630
MboII GAAGA 3 cut(s) 171, 578, 668
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 2 cut(s) 460, 679
MluCI AATT 3 cut(s) 96, 263, 599
MlyI GAGTC 2 cut(s) 14, 544
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 6 cut(s) 290, 359, 422, 631, 644, 702
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 2 cut(s) 369, 521
MslI CAYNNNNRTG 2 cut(s) 249, 699
MspI CCGG 2 cut(s) 290, 653
MspR9I CCNGG 2 cut(s) 405, 621
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 405, 621
MwoI GCNNNNNNNGC 1 cut(s) 577
NdeII GATC 4 cut(s) 170, 270, 316, 630
NlaIII CATG 5 cut(s) 161, 248, 317, 465, 598
NlaIV GGNNCC 1 cut(s) 690
PaeR7I CTCGAG 1 cut(s) 635
PagI TCATGA 1 cut(s) 313
PceI AGGCCT 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 561
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 2 cut(s) 147, 408
PflMI CCANNNNNTGG 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 1 cut(s) 133
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 2 cut(s) 13, 544
PpsI GAGTC 2 cut(s) 13, 544
PpuMI RGGWCCY 1 cut(s) 689
Psp5II RGGWCCY 1 cut(s) 689
Psp6I CCWGG 2 cut(s) 403, 619
PspGI CCWGG 2 cut(s) 403, 619
PspN4I GGNNCC 1 cut(s) 690
PspPI GGNCC 1 cut(s) 689
PspPPI RGGWCCY 1 cut(s) 689
PspXI VCTCGAGB 1 cut(s) 635
PsrI GAACNNNNNNTAC 2 cut(s) 633, 665
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 170
PvuI CGATCG 1 cut(s) 273
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 2 cut(s) 249, 699
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 2 cut(s) 369, 521
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 4 cut(s) 170, 270, 316, 630
Sau96I GGNCC 1 cut(s) 689
SbfI CCTGCAGG 1 cut(s) 56
SchI GAGTC 2 cut(s) 14, 544
ScrFI CCNGG 2 cut(s) 405, 621
SdaI CCTGCAGG 1 cut(s) 56
SduI GDGCHC 2 cut(s) 460, 679
SetI ASST 7 cut(s) 81, 376, 393, 438, 473, 573, 663
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 635
SinI GGWCC 1 cut(s) 689
SlaI CTCGAG 1 cut(s) 635
SmiMI CAYNNNNRTG 2 cut(s) 249, 699
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse8387I CCTGCAGG 1 cut(s) 56
Sse9I AATT 3 cut(s) 96, 263, 599
SseBI AGGCCT 1 cut(s) 580
SspI AATATT 1 cut(s) 502
SspMI CTAG 2 cut(s) 183, 662
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 2 cut(s) 403, 619
TaaI ACNGT 4 cut(s) 194, 334, 356, 728
TaiI ACGT 1 cut(s) 473
TaqI TCGA 5 cut(s) 150, 269, 393, 548, 636
TasI AATT 3 cut(s) 96, 263, 599
TfiI GAWTC 2 cut(s) 147, 408
Tru1I TTAA 2 cut(s) 369, 521
Tru9I TTAA 2 cut(s) 369, 521
TscAI CASTG 1 cut(s) 199
TseI GCWGC 1 cut(s) 131
TspDTI ATGAA 5 cut(s) 168, 233, 267, 302, 583
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 2 cut(s) 96, 599
XhoI CTCGAG 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 580
XspI CTAG 2 cut(s) 183, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.