Rh7DG457000

PCO_ADO

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0018048
Physical Location & Seq
Forward (+)
64954493 .. 64956200
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG457000.1

Sequence Viewer

Length: 732 bp
ATGGAGTCGGGTACAATGGAGAAAACCAAAATACAGATGCTATACGAAGCCTGCAGGGTTATATTTTCTCAGAAAGAGCTTCCAACTTTCAAAGAAATTCACTGGCTCAAAAATCTCCTGGGCACATTTGAAGCAATAGATTTTGGAATCGATGAACATGGCTTGTGTGGATCTCCATCTTCTAGTCCAAAGAGTGATAAGGGATTGATTTGTGGACAAGGCATCTCCCAGATCACTTACATTCATGTTTATGAATGTGAACAATTCTCTATCGGCGTGTTTTGTTTCCCGACAGGAGGGATGCTTCCCCTTCATGATCACCCAGGAATGACCGTGTTTAGCAAACTCCTTTATGGTTCTTGTTATGTTAAAGCTTACGACTGGGTGAAGGTCGAAAGTTCTCCTGGCTTCCCAACATTTGGATTAGGAGGCAAGGTTTTGGATGGCATTATGAGTGCTCCATGTGAAACTTCTGTTCTATTTCCAAGAAGCGGTGGAAATATTCACGCTTTTACTGCGTTAACTCCTTGTGCGATCTTGGATGTATTGACTCCACCATACTCGGAGGAGCTTGGAAGGCCTTCTACTTACTTCATGGAATTTCCTATTCCTTCCTTGCCAGGCTATACGATCCTTGAGGAACGAGACCTACCGGATGACCTAGTTGTTGAAGGAGCACCATATCTTGGCCCTCCTATTGAAGCTGATGATGATGACAACGACCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

243

Amino Acids

26.68

Weight (kDa)

4.57

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 72 - 233 3.2e-56 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018048)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36720
malus_domestica MD15G1373300.v1.1
prunus_persica Prupe.1G521700_v2.0.a1
pyrus_communis pycom15g33420
rosa_chinensis RchiOBHm_Chr7g0242701
rosa_laevigata RLG00000000886
rosa_roxburghii Rroxscaffold_3G00222570
rosa_rugosa Rorug07G0316400
rosa_samantha Rh7AG471500 Rh7DG457000
rosa_wichuraiana Rw7G039220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 419, 686
AciI CCGC 1 cut(s) 492
AclWI GGATC 2 cut(s) 178, 625
AcsI RAATTY 2 cut(s) 96, 599
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
AgsI TTSAA 4 cut(s) 91, 131, 671, 701
AjnI CCWGG 4 cut(s) 117, 322, 403, 619
AluBI AGCT 4 cut(s) 79, 374, 571, 704
AluI AGCT 4 cut(s) 79, 374, 571, 704
Alw21I GWGCWC 2 cut(s) 460, 679
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 2 cut(s) 178, 625
AoxI GGCC 2 cut(s) 578, 688
ApoI RAATTY 2 cut(s) 96, 599
Asp700I GAANNNNTTC 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 689
AsuHPI GGTGA 2 cut(s) 311, 397
BaeGI GKGCMC 1 cut(s) 125
BarI GAAGNNNNNNTAC 2 cut(s) 568, 600
Bbv12I GWGCWC 2 cut(s) 460, 679
BccI CCATC 2 cut(s) 184, 437
BciT130I CCWGG 4 cut(s) 119, 324, 405, 621
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 2 cut(s) 183, 662
BfmI CTRYAG 1 cut(s) 52
Bme1390I CCNGG 4 cut(s) 119, 324, 405, 621
BmgT120I GGNCC 1 cut(s) 689
BmrFI CCNGG 4 cut(s) 119, 324, 405, 621
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 27, 231, 291
BmuI ACTGGG 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 656
Bsa29I ATCGAT 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 175
BsaI GGTCTC 1 cut(s) 639
BsaJI CCNNGG 2 cut(s) 118, 322
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
Bse1I ACTGG 2 cut(s) 107, 386
Bse8I GATNNNNATC 1 cut(s) 175
BseBI CCWGG 4 cut(s) 119, 324, 405, 621
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 118, 322
BseGI GGATG 4 cut(s) 306, 448, 547, 661
BseJI GATNNNNATC 1 cut(s) 175
BseLI CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 107, 386
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 125
BshFI GGCC 2 cut(s) 580, 690
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 460, 679
BsiSI CCGG 1 cut(s) 653
BslI CCNNNNNNNGG 4 cut(s) 296, 419, 491, 686
BsmAI GTCTC 1 cut(s) 639
BsnI GGCC 2 cut(s) 580, 690
Bso31I GGTCTC 1 cut(s) 639
Bsp1286I GDGCHC 3 cut(s) 125, 460, 679
Bsp143I GATC 5 cut(s) 170, 231, 316, 534, 630
BspACI CCGC 1 cut(s) 492
BspANI GGCC 2 cut(s) 580, 690
BspCNI CTCAG 1 cut(s) 82
BspDI ATCGAT 1 cut(s) 150
BspHI TCATGA 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 178, 625
BspTNI GGTCTC 1 cut(s) 639
BsrI ACTGG 2 cut(s) 107, 386
BssECI CCNNGG 2 cut(s) 118, 322
BssMI GATC 5 cut(s) 170, 231, 316, 534, 630
Bst2UI CCWGG 4 cut(s) 119, 324, 405, 621
Bst4CI ACNGT 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 4 cut(s) 306, 448, 547, 661
BstKTI GATC 5 cut(s) 173, 234, 319, 537, 633
BstMAI GTCTC 1 cut(s) 639
BstMBI GATC 5 cut(s) 170, 231, 316, 534, 630
BstMWI GCNNNNNNNGC 2 cut(s) 515, 577
BstNI CCWGG 4 cut(s) 119, 324, 405, 621
BstSCI CCNGG 4 cut(s) 117, 322, 403, 619
BstSFI CTRYAG 1 cut(s) 52
BstSLI GKGCMC 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 150
BsuRI GGCC 2 cut(s) 580, 690
BsuTUI ATCGAT 1 cut(s) 150
BtsCI GGATG 4 cut(s) 306, 448, 547, 661
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 52
CciI TCATGA 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 689
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 1 cut(s) 12
CviAII CATG 5 cut(s) 158, 245, 314, 462, 595
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 1 cut(s) 69
DpnI GATC 5 cut(s) 172, 233, 318, 536, 632
DpnII GATC 5 cut(s) 170, 231, 316, 534, 630
Eco147I AGGCCT 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 639
EcoRII CCWGG 4 cut(s) 117, 322, 403, 619
FaeI CATG 5 cut(s) 161, 248, 317, 465, 598
FatI CATG 5 cut(s) 157, 244, 313, 461, 594
FbaI TGATCA 1 cut(s) 316
FokI GGATG 4 cut(s) 313, 455, 554, 668
FspBI CTAG 2 cut(s) 183, 662
HaeIII GGCC 2 cut(s) 580, 690
HapII CCGG 1 cut(s) 653
Hin1II CATG 5 cut(s) 161, 248, 317, 465, 598
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 3 cut(s) 5, 147, 550
HpaI GTTAAC 1 cut(s) 522
HpaII CCGG 1 cut(s) 653
HphI GGTGA 2 cut(s) 311, 397
Hpy166II GTNNAC 3 cut(s) 215, 260, 522
Hpy188I TCNGA 2 cut(s) 72, 565
Hpy188III TCNNGA 2 cut(s) 289, 314
Hpy8I GTNNAC 3 cut(s) 215, 260, 522
HpyAV CCTTC 6 cut(s) 320, 382, 570, 591, 621, 665
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4V TGCA 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 577
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 5 cut(s) 161, 248, 317, 465, 598
Ksp22I TGATCA 1 cut(s) 316
KspAI GTTAAC 1 cut(s) 522
Kzo9I GATC 5 cut(s) 170, 231, 316, 534, 630
LmnI GCTCC 3 cut(s) 463, 568, 674
LweI GCATC 3 cut(s) 27, 231, 291
MaeI CTAG 2 cut(s) 183, 662
MalI GATC 5 cut(s) 172, 233, 318, 536, 632
MboI GATC 5 cut(s) 170, 231, 316, 534, 630
MboII GAAGA 1 cut(s) 171
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 3 cut(s) 125, 460, 679
MluCI AATT 3 cut(s) 96, 263, 599
MlyI GAGTC 2 cut(s) 14, 544
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 5 cut(s) 290, 422, 559, 631, 702
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 2 cut(s) 369, 521
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 4 cut(s) 119, 324, 405, 621
MvaI CCWGG 4 cut(s) 119, 324, 405, 621
MwoI GCNNNNNNNGC 2 cut(s) 515, 577
NdeII GATC 5 cut(s) 170, 231, 316, 534, 630
NlaIII CATG 5 cut(s) 161, 248, 317, 465, 598
PagI TCATGA 1 cut(s) 313
PceI AGGCCT 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 1 cut(s) 147
PflMI CCANNNNNTGG 2 cut(s) 419, 686
PleI GAGTC 2 cut(s) 13, 544
PpsI GAGTC 2 cut(s) 13, 544
Psp6I CCWGG 4 cut(s) 117, 322, 403, 619
PspGI CCWGG 4 cut(s) 117, 322, 403, 619
PspPI GGNCC 1 cut(s) 689
PsrI GAACNNNNNNTAC 2 cut(s) 633, 665
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 170
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 2 cut(s) 369, 521
Sau3AI GATC 5 cut(s) 170, 231, 316, 534, 630
Sau96I GGNCC 1 cut(s) 689
SbfI CCTGCAGG 1 cut(s) 56
SchI GAGTC 2 cut(s) 14, 544
ScrFI CCNGG 4 cut(s) 119, 324, 405, 621
SdaI CCTGCAGG 1 cut(s) 56
SduI GDGCHC 3 cut(s) 125, 460, 679
SetI ASST 8 cut(s) 81, 376, 393, 438, 573, 651, 663, 706
SfaNI GCATC 3 cut(s) 27, 231, 291
SfcI CTRYAG 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse8387I CCTGCAGG 1 cut(s) 56
Sse9I AATT 3 cut(s) 96, 263, 599
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 1 cut(s) 492
SspI AATATT 1 cut(s) 502
SspMI CTAG 2 cut(s) 183, 662
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 4 cut(s) 117, 322, 403, 619
TaaI ACNGT 1 cut(s) 334
TaqI TCGA 2 cut(s) 150, 393
TasI AATT 3 cut(s) 96, 263, 599
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 2 cut(s) 369, 521
Tru9I TTAA 2 cut(s) 369, 521
TscAI CASTG 1 cut(s) 107
TspDTI ATGAA 5 cut(s) 168, 233, 267, 302, 583
TspRI CASTG 1 cut(s) 107
Van91I CCANNNNNTGG 2 cut(s) 419, 686
XapI RAATTY 2 cut(s) 96, 599
XmnI GAANNNNTTC 1 cut(s) 580
XspI CTAG 2 cut(s) 183, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.