pycom15g33420

PCO_ADO

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0018048
Physical Location & Seq
Reverse (-)
32865018 .. 32866284
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33420.4

Sequence Viewer

Length: 768 bp
ATGGACTGCATAATATTGGAGAAAAGCAAAATACAAATGCTGTTTGAAGCCTGCAATGTCATCTTTTCGGATCCGAAAGCACTCCCAACTTTCCGACAAATCCAATGGCTGAAAAATCTCCTTGGCACATTTGAAGCCATAGATTTCGGAATCGATGAATTTGGTTCAGGTAGATCTCCATCTTCGAGTCCAAGGAGCTGCAAGGGATTGGTTTCTGGGCAAGGCATCTCCCAAATCACTTACATTCATGTTCATGAATGTGACCATTTCTCTATAGGCGTGTTTTGCTTCCCGGCTGGCGCTACGCTTCCCCTTCATGATCACCCTCGAATGACAGTCTTTACCAAACTCCTCTACGGTTCTGTTTATGTTAAATCTTACGATTGGATCAAAGTGGAAACTTCTTCTAGCTTCAGAACATTTGGATTGGCAGGCAAGGTTTTTGATGGAGTTATAAGCTCACCATGTGAGACTCGTGTATTATTTCCAAGAAGTGGCGGGAACATTCATTCTTTTACAGCAGTAACTCCCTGTGCTATCTTGGATGTACTGACGCCGCCTTACTCCGATGACCATGGCAGGCCTTCTACTTACTTCTTCGACTTCCCTATTCCATCTCTTCCTGTCATGGCTAATCTGTTACTGTGTCCAATTTATGGAGCAGCAGGCTATGCAGTGCTTGAGGAAAGGGACATGCCCGAAGATCTGTTTGTTGCTGGAGCGCCATATCTTGGCCCTCCTATTCAAGCGTGTGATGACCGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

256

Amino Acids

28.05

Weight (kDa)

5.44

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018048)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36720
malus_domestica MD15G1373300.v1.1
prunus_persica Prupe.1G521700_v2.0.a1
pyrus_communis pycom15g33420
rosa_chinensis RchiOBHm_Chr7g0242701
rosa_laevigata RLG00000000886
rosa_roxburghii Rroxscaffold_3G00222570
rosa_rugosa Rorug07G0316400
rosa_samantha Rh7AG471500 Rh7DG457000
rosa_wichuraiana Rw7G039220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AccB7I CCANNNNNTGG 3 cut(s) 494, 656, 731
AciI CCGC 2 cut(s) 498, 557
AclWI GGATC 3 cut(s) 65, 78, 395
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 397
AcyI GRCGYC 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 467
AfaI GTAC 1 cut(s) 549
AfiI CCNNNNNNNGG 3 cut(s) 494, 656, 731
AgsI TTSAA 3 cut(s) 47, 134, 746
AluBI AGCT 3 cut(s) 198, 411, 459
AluI AGCT 3 cut(s) 198, 411, 459
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 3 cut(s) 65, 78, 395
AoxI GGCC 2 cut(s) 581, 733
ApeKI GCWGC 2 cut(s) 198, 662
ApoI RAATTY 1 cut(s) 158
AspLEI GCGC 2 cut(s) 302, 724
AspS9I GGNCC 1 cut(s) 734
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 314, 453
BamHI GGATCC 1 cut(s) 70
BauI CACGAG 1 cut(s) 474
BbvI GCAGC 2 cut(s) 185, 674
BccI CCATC 3 cut(s) 187, 440, 622
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 273
BfoI RGCGCY 2 cut(s) 303, 725
BglII AGATCT 2 cut(s) 173, 703
BisI GCNGC 3 cut(s) 199, 557, 663
BlsI GCNGC 3 cut(s) 200, 558, 664
Bme1390I CCNGG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 738
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 554
BsaJI CCNNGG 3 cut(s) 121, 191, 574
Bsc4I CCNNNNNNNGG 3 cut(s) 494, 656, 731
Bse3DI GCAATG 1 cut(s) 61
Bse8I GATNNNNATC 1 cut(s) 178
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 121, 191, 574
BseGI GGATG 1 cut(s) 550
BseJI GATNNNNATC 1 cut(s) 178
BseLI CCNNNNNNNGG 3 cut(s) 494, 656, 731
BseMI GCAATG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 2 cut(s) 185, 674
BshFI GGCC 2 cut(s) 583, 735
BshVI ATCGAT 1 cut(s) 153
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 3 cut(s) 494, 656, 731
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 704
BsnI GGCC 2 cut(s) 583, 735
Bsp143I GATC 5 cut(s) 70, 173, 319, 387, 703
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 2 cut(s) 498, 557
BspANI GGCC 2 cut(s) 583, 735
BspDI ATCGAT 1 cut(s) 153
BspHI TCATGA 2 cut(s) 253, 316
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 3 cut(s) 65, 78, 395
BsrDI GCAATG 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 121, 191, 574
BssMI GATC 5 cut(s) 70, 173, 319, 387, 703
BssNI GRCGYC 1 cut(s) 554
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 3 cut(s) 121, 191, 574
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 4 cut(s) 337, 359, 645, 761
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 671
BstC8I GCNNGC 5 cut(s) 52, 298, 433, 581, 667
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 1 cut(s) 550
BstH2I RGCGCY 2 cut(s) 303, 725
BstHHI GCGC 2 cut(s) 302, 724
BstKTI GATC 5 cut(s) 73, 176, 322, 390, 706
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 5 cut(s) 70, 173, 319, 387, 703
BstMWI GCNNNNNNNGC 2 cut(s) 285, 671
BstNSI RCATGY 1 cut(s) 697
BstSCI CCNGG 1 cut(s) 291
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 185, 674
BstX2I RGATCY 3 cut(s) 70, 173, 703
BstYI RGATCY 3 cut(s) 70, 173, 703
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 2 cut(s) 583, 735
BsuTUI ATCGAT 1 cut(s) 153
BtgI CCRYGG 1 cut(s) 574
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 5 cut(s) 52, 298, 433, 581, 667
CciI TCATGA 2 cut(s) 253, 316
CfoI GCGC 2 cut(s) 302, 724
Cfr13I GGNCC 1 cut(s) 734
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 1 cut(s) 562
Csp6I GTAC 1 cut(s) 548
CviAII CATG 7 cut(s) 248, 254, 317, 465, 575, 628, 694
CviQI GTAC 1 cut(s) 548
DpnI GATC 5 cut(s) 72, 175, 321, 389, 705
DpnII GATC 5 cut(s) 70, 173, 319, 387, 703
DraIII CACNNNGTG 1 cut(s) 467
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 3 cut(s) 121, 191, 574
Eco147I AGGCCT 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 397
EcoT14I CCWWGG 3 cut(s) 121, 191, 574
ErhI CCWWGG 3 cut(s) 121, 191, 574
FaeI CATG 7 cut(s) 251, 257, 320, 468, 578, 631, 697
FaqI GGGAC 1 cut(s) 704
FatI CATG 7 cut(s) 247, 253, 316, 464, 574, 627, 693
FauI CCCGC 1 cut(s) 491
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 199, 557, 663
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 3 cut(s) 199, 557, 663
FspBI CTAG 1 cut(s) 408
GlaI GCGC 2 cut(s) 301, 723
GluI GCNGC 3 cut(s) 199, 557, 663
GsuI CTGGAG 1 cut(s) 738
HaeII RGCGCY 2 cut(s) 303, 725
HaeIII GGCC 2 cut(s) 583, 735
HapII CCGG 1 cut(s) 293
HgaI GACGC 1 cut(s) 562
HhaI GCGC 2 cut(s) 302, 724
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 7 cut(s) 251, 257, 320, 468, 578, 631, 697
Hin6I GCGC 2 cut(s) 300, 722
HinP1I GCGC 2 cut(s) 300, 722
HinfI GANTC 3 cut(s) 150, 187, 472
HpaII CCGG 1 cut(s) 293
HphI GGTGA 2 cut(s) 314, 453
Hpy188I TCNGA 6 cut(s) 70, 75, 95, 149, 416, 568
Hpy188III TCNNGA 2 cut(s) 254, 317
HpyAV CCTTC 2 cut(s) 323, 594
HpyCH4III ACNGT 4 cut(s) 337, 359, 645, 761
HpyCH4V TGCA 4 cut(s) 9, 54, 201, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 671
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 7 cut(s) 251, 257, 320, 468, 578, 631, 697
HspAI GCGC 2 cut(s) 300, 722
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 70, 173, 319, 387, 703
LmnI GCTCC 3 cut(s) 195, 659, 719
Lsp1109I GCAGC 2 cut(s) 185, 674
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 3 cut(s) 260, 523, 639
MalI GATC 5 cut(s) 72, 175, 321, 389, 705
MboI GATC 5 cut(s) 70, 173, 319, 387, 703
MboII GAAGA 5 cut(s) 174, 396, 589, 611, 713
MflI RGATCY 3 cut(s) 70, 173, 703
MluCI AATT 2 cut(s) 158, 651
MlyI GAGTC 2 cut(s) 196, 466
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 4 cut(s) 336, 362, 676, 747
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 2 cut(s) 252, 258
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 285, 671
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 1 cut(s) 574
NdeII GATC 5 cut(s) 70, 173, 319, 387, 703
NlaIII CATG 7 cut(s) 251, 257, 320, 468, 578, 631, 697
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 260
NspI RCATGY 1 cut(s) 697
PagI TCATGA 2 cut(s) 253, 316
PceI AGGCCT 1 cut(s) 583
PfeI GAWTC 1 cut(s) 150
PflMI CCANNNNNTGG 3 cut(s) 494, 656, 731
PkrI GCNGC 3 cut(s) 200, 558, 664
PleI GAGTC 2 cut(s) 195, 466
PpsI GAGTC 2 cut(s) 195, 466
PsiI TTATAA 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 3 cut(s) 70, 173, 703
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
RseI CAYNNNNRTG 2 cut(s) 252, 258
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 199, 557, 663
Sau3AI GATC 5 cut(s) 70, 173, 319, 387, 703
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 2 cut(s) 196, 466
ScrFI CCNGG 1 cut(s) 293
SetI ASST 5 cut(s) 172, 200, 413, 441, 461
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 1 cut(s) 273
SmiMI CAYNNNNRTG 2 cut(s) 252, 258
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 2 cut(s) 158, 651
SseBI AGGCCT 1 cut(s) 583
SsiI CCGC 2 cut(s) 498, 557
SspI AATATT 1 cut(s) 15
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 583
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 3 cut(s) 121, 191, 574
TaaI ACNGT 4 cut(s) 337, 359, 645, 761
TaqI TCGA 4 cut(s) 153, 185, 328, 600
TasI AATT 2 cut(s) 158, 651
TatI WGTACW 1 cut(s) 547
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 681
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 2 cut(s) 198, 662
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 6 cut(s) 171, 236, 242, 270, 305, 497
TspRI CASTG 1 cut(s) 681
Van91I CCANNNNNTGG 3 cut(s) 494, 656, 731
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 697
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.