MD15G1373300.v1.1

PCO_ADO

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0018048
Physical Location & Seq
Reverse (-)
45568641 .. 45569955
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1373300.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGACTGCATAATATTGGAGAAAAGCAAAATACAAATGCTGTATAAAGCCTGCAATGTCGTCTTTTCGGATCTGAAAGCACTCCCAACTTTCCGACAAATCCAATGGCTGAAAAATCTCCTTGGCACATTTGAAGCCATAGATTTCGGAATCGATGAATTTGGTTCGGGTAGATCTCCATCTTCGAGTCCAAGGAGCGGCCAGGGATTGGTTTCTGGGCAAGGCATCTCCCAAATCACTTACATTCATGTTCATGAATGTGACCATTTCTCTATAGGCGTGTTTTGCTTCCCGGCTGGGGCTACGCTTCCCCTTCATGATCACCCTGGAATGACAGTCTTTACCAAACTCCTCTACGGTTCTGTTTATTTTAAATCTTACGATTGGATCAAAGTGGAAACTTCTTCTAGCTTCAGAACATTTGGATTGGCAGGCAAGGTTTTTGATGGAGTTATAAGCTCACCATGTGAGACTCGTGTATTATTTCCAAGAAGTGGCGGGAACATTCATTCTTTTACTGCAGTAACTCCCTGTGCTATCTTGGATGTATTGACCCCACCTTACTCCGACGACCACGGCAGGCCTTCTACTTACTTCTTCGACTTCCCTATTCCATCTCTTCCTGGCTATGCGGTGCTTGAGGAAAGGGACATGCCCGAAGATCTGTTTGTTGCTGGAGCGCCATATCTTGGCCCTCCTATTCAAGCGTGTGATGACCGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

242

Amino Acids

26.54

Weight (kDa)

5.42

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 68 - 234 5.3e-58 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018048)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36720
malus_domestica MD15G1373300.v1.1
prunus_persica Prupe.1G521700_v2.0.a1
pyrus_communis pycom15g33420
rosa_chinensis RchiOBHm_Chr7g0242701
rosa_laevigata RLG00000000886
rosa_roxburghii Rroxscaffold_3G00222570
rosa_rugosa Rorug07G0316400
rosa_samantha Rh7AG471500 Rh7DG457000
rosa_wichuraiana Rw7G039220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AccB7I CCANNNNNTGG 3 cut(s) 208, 494, 689
AccBSI CCGCTC 1 cut(s) 198
AciI CCGC 3 cut(s) 198, 498, 632
AclWI GGATC 2 cut(s) 78, 395
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 397
AdeI CACNNNGTG 1 cut(s) 467
AfiI CCNNNNNNNGG 5 cut(s) 197, 208, 298, 494, 689
AgsI TTSAA 2 cut(s) 134, 704
AjnI CCWGG 3 cut(s) 201, 325, 622
AluBI AGCT 2 cut(s) 411, 459
AluI AGCT 2 cut(s) 411, 459
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 2 cut(s) 78, 395
AoxI GGCC 3 cut(s) 199, 581, 691
ApoI RAATTY 1 cut(s) 158
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 1 cut(s) 692
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 314, 453
BauI CACGAG 1 cut(s) 474
BccI CCATC 3 cut(s) 187, 440, 622
BceAI ACGGC 1 cut(s) 592
BciT130I CCWGG 3 cut(s) 203, 327, 624
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 273, 519
BfoI RGCGCY 1 cut(s) 683
BglII AGATCT 2 cut(s) 173, 661
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 4 cut(s) 203, 293, 327, 624
BmgT120I GGNCC 1 cut(s) 692
BmrFI CCNGG 4 cut(s) 203, 293, 327, 624
BmsI GCATC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 178
BsaJI CCNNGG 5 cut(s) 121, 191, 202, 325, 574
Bsc4I CCNNNNNNNGG 5 cut(s) 197, 208, 298, 494, 689
Bse3DI GCAATG 1 cut(s) 61
Bse8I GATNNNNATC 1 cut(s) 178
BseBI CCWGG 3 cut(s) 203, 327, 624
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 5 cut(s) 121, 191, 202, 325, 574
BseGI GGATG 1 cut(s) 550
BseJI GATNNNNATC 1 cut(s) 178
BseLI CCNNNNNNNGG 5 cut(s) 197, 208, 298, 494, 689
BseMI GCAATG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 341
BseYI CCCAGC 1 cut(s) 296
BshFI GGCC 3 cut(s) 201, 583, 693
BshVI ATCGAT 1 cut(s) 153
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 5 cut(s) 197, 208, 298, 494, 689
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 662
BsnI GGCC 3 cut(s) 201, 583, 693
Bsp143I GATC 5 cut(s) 70, 173, 319, 387, 661
BspACI CCGC 3 cut(s) 198, 498, 632
BspANI GGCC 3 cut(s) 201, 583, 693
BspDI ATCGAT 1 cut(s) 153
BspHI TCATGA 2 cut(s) 253, 316
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 2 cut(s) 78, 395
BsrBI CCGCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 61
BssECI CCNNGG 5 cut(s) 121, 191, 202, 325, 574
BssMI GATC 5 cut(s) 70, 173, 319, 387, 661
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 2 cut(s) 121, 191
Bst2BI CACGAG 1 cut(s) 474
Bst2UI CCWGG 3 cut(s) 203, 327, 624
Bst4CI ACNGT 3 cut(s) 337, 359, 719
Bst6I CTCTTC 1 cut(s) 624
BstC8I GCNNGC 3 cut(s) 52, 433, 581
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 1 cut(s) 550
BstH2I RGCGCY 1 cut(s) 683
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 5 cut(s) 73, 176, 322, 390, 664
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 5 cut(s) 70, 173, 319, 387, 661
BstMWI GCNNNNNNNGC 1 cut(s) 285
BstNI CCWGG 3 cut(s) 203, 327, 624
BstNSI RCATGY 1 cut(s) 655
BstSCI CCNGG 4 cut(s) 201, 291, 325, 622
BstSFI CTRYAG 2 cut(s) 273, 519
BstX2I RGATCY 3 cut(s) 70, 173, 661
BstYI RGATCY 3 cut(s) 70, 173, 661
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 3 cut(s) 201, 583, 693
BsuTUI ATCGAT 1 cut(s) 153
BtgI CCRYGG 1 cut(s) 574
BtsCI GGATG 1 cut(s) 550
Cac8I GCNNGC 3 cut(s) 52, 433, 581
CciI TCATGA 2 cut(s) 253, 316
CfoI GCGC 1 cut(s) 682
Cfr13I GGNCC 1 cut(s) 692
ClaI ATCGAT 1 cut(s) 153
CviAII CATG 5 cut(s) 248, 254, 317, 465, 652
DpnI GATC 5 cut(s) 72, 175, 321, 389, 663
DpnII GATC 5 cut(s) 70, 173, 319, 387, 661
DraI TTTAAA 1 cut(s) 373
DraIII CACNNNGTG 1 cut(s) 467
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 121, 191
Eco147I AGGCCT 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 397
EcoRII CCWGG 3 cut(s) 201, 325, 622
EcoT14I CCWWGG 2 cut(s) 121, 191
ErhI CCWWGG 2 cut(s) 121, 191
FaeI CATG 5 cut(s) 251, 257, 320, 468, 655
FaqI GGGAC 1 cut(s) 662
FatI CATG 5 cut(s) 247, 253, 316, 464, 651
FauI CCCGC 1 cut(s) 491
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 1 cut(s) 199
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 199
FspBI CTAG 1 cut(s) 408
GlaI GCGC 1 cut(s) 681
GluI GCNGC 1 cut(s) 199
GsaI CCCAGC 1 cut(s) 300
GsuI CTGGAG 1 cut(s) 696
HaeII RGCGCY 1 cut(s) 683
HaeIII GGCC 3 cut(s) 201, 583, 693
HapII CCGG 1 cut(s) 293
HhaI GCGC 1 cut(s) 682
Hin1II CATG 5 cut(s) 251, 257, 320, 468, 655
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HinfI GANTC 3 cut(s) 150, 187, 472
HpaII CCGG 1 cut(s) 293
HphI GGTGA 2 cut(s) 314, 453
Hpy188I TCNGA 6 cut(s) 70, 75, 95, 149, 416, 568
Hpy188III TCNNGA 2 cut(s) 254, 317
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 2 cut(s) 323, 594
HpyCH4III ACNGT 3 cut(s) 337, 359, 719
HpyCH4V TGCA 3 cut(s) 9, 54, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 285
Hsp92II CATG 5 cut(s) 251, 257, 320, 468, 655
HspAI GCGC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 70, 173, 319, 387, 661
LmnI GCTCC 2 cut(s) 195, 677
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 260, 523
MalI GATC 5 cut(s) 72, 175, 321, 389, 663
MbiI CCGCTC 1 cut(s) 198
MboI GATC 5 cut(s) 70, 173, 319, 387, 661
MboII GAAGA 5 cut(s) 174, 396, 589, 611, 671
MflI RGATCY 3 cut(s) 70, 173, 661
MluCI AATT 1 cut(s) 158
MlyI GAGTC 2 cut(s) 196, 466
MmeI TCCRAC 2 cut(s) 118, 591
MnlI CCTC 3 cut(s) 362, 634, 705
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 2 cut(s) 252, 258
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 4 cut(s) 203, 293, 327, 624
MvaI CCWGG 3 cut(s) 203, 327, 624
MwoI GCNNNNNNNGC 1 cut(s) 285
NciI CCSGG 1 cut(s) 293
NdeII GATC 5 cut(s) 70, 173, 319, 387, 661
NlaIII CATG 5 cut(s) 251, 257, 320, 468, 655
NmuCI GTSAC 1 cut(s) 260
NspI RCATGY 1 cut(s) 655
PagI TCATGA 2 cut(s) 253, 316
PceI AGGCCT 1 cut(s) 583
PfeI GAWTC 1 cut(s) 150
PflMI CCANNNNNTGG 3 cut(s) 208, 494, 689
PkrI GCNGC 1 cut(s) 200
PleI GAGTC 2 cut(s) 195, 466
PpsI GAGTC 2 cut(s) 195, 466
PsiI TTATAA 1 cut(s) 455
Psp6I CCWGG 3 cut(s) 201, 325, 622
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 3 cut(s) 201, 325, 622
PspPI GGNCC 1 cut(s) 692
PstI CTGCAG 1 cut(s) 523
PsuI RGATCY 3 cut(s) 70, 173, 661
RseI CAYNNNNRTG 2 cut(s) 252, 258
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 5 cut(s) 70, 173, 319, 387, 661
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 2 cut(s) 196, 466
ScrFI CCNGG 4 cut(s) 203, 293, 327, 624
SetI ASST 4 cut(s) 413, 441, 461, 562
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 2 cut(s) 273, 519
SmiMI CAYNNNNRTG 2 cut(s) 252, 258
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 1 cut(s) 158
SseBI AGGCCT 1 cut(s) 583
SsiI CCGC 3 cut(s) 198, 498, 632
SspI AATATT 1 cut(s) 15
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 583
StyD4I CCNGG 4 cut(s) 201, 291, 325, 622
StyI CCWWGG 2 cut(s) 121, 191
TaaI ACNGT 3 cut(s) 337, 359, 719
TaqI TCGA 3 cut(s) 153, 185, 600
TasI AATT 1 cut(s) 158
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 6 cut(s) 171, 236, 242, 270, 305, 497
Van91I CCANNNNNTGG 3 cut(s) 208, 494, 689
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 655
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.